WO2023213325A1 - High-energy beverage prepared by combining and synergistically refining ultra-high-purity octacosanol-flavonoid compound and deuterated water, and preparation method therefor - Google Patents

Abstract

A high-energy beverage prepared by combining and synergistically refining an ultra-high-purity octacosanol-flavonoid compound and deuterated water, and a preparation method therefor, comprising the following components: octacosanol, flavonoids, deuterated water, a stabiliser, a compound emulsifier, water-soluble dietary fibre, compound electrolytes, a preservative, and an acidulant; the octacosanol is octacosanol with a purity of ≥98% extracted from fresh sugarcane filter mud, the flavonoids are bamboo leaf flavonoids with a purity of ≥95%, and the deuterium content of the deuterated water is 25-100 ppm.

Description

An ultra-high-purity octacosanol-flavonoid compound deuterated water combination synergistically refined high-energy drink and its preparation method Technical field

The invention relates to the technical field of food processing, and specifically designs a high-energy beverage that is synergistically refined with an ultra-high-purity octacosanol-flavonoid composite deuterated water combination and a preparation method thereof.

Background technique

⒈Octacosanol is a natural higher fatty alcohol with good biodegradability and easy to be absorbed by the human body.

⑴ Beginning in 1949, Dr. T.K. Cureton of the University of Illinois in the United States and others spent 20 years testing 42 items on 894 subjects. Participants included swimming, wrestling, track and field team members, etc. Physical education students, U.S. Navy, Navy divers, etc. Summarizing these experimental results, octacosanol has the effects of metabolic rate, reducing muscle friction, thereby reducing necessary oxygen demand, improving reaction time, eliminating muscle pain, etc., and these effects are interrelated; twenty-eight octasanol was determined The main functions of alkanols in actively promoting human physiological functions are:

① Enhance endurance, energy and physical strength;

② Improve muscle strength (muscle function); eliminate muscle pain and reduce muscle friction;

③Shorten muscle and nerve reaction time, improve stress ability; improve reaction acuity;

④Strengthen heart function and improve myocardial function;

⑤Improve stress ability and body metabolism rate;

⑥Enhance resistance to stress such as high mountains;

⑦Reduce systolic blood pressure;

⑧Improve oxygen transport capacity, body oxygen utilization rate, and reduce necessary oxygen demand;

⑨ Stimulating hormones;

⑩Reduce cholesterol;

⑵The "three highs" are a type of disease with a high incidence that threatens modern human health.

As early as 2006, Zhong Geng and others had confirmed that octacosanol has the function of lowering blood lipids. Qi Xiaoming and others proposed that the hypolipidemia effect of octacosanol is related to the dosage. Higher dosages of this substance can significantly reduce the TG, TC and LDL-C content in the serum; while low dosages only have an effect on the serum lipid levels. TC concentration has an impact. Research by DevK.Singh et al. proposed that octacosanol reduces cholesterol content in serum by activating enzyme activity through phosphorylation of AMP-kinase, thereby reducing HMG-CoA enzyme activity and controlling cholesterol synthesis in serum. Purpose;

⑶ Octacosanol can reduce excess calcium and fat content in the blood and is a promoter of calcitonin formation.

It can be used to treat hypercalcemia, osteoporosis, high cholesterol and high lipoprotein blood type diseases, and can be used to treat Parkinson's disease in the early stages of the elderly. It has been confirmed by many parties that octacosanol is known as a compound that can relieve physical fatigue and has good biological activity. Research shows that this substance can improve the human body's endurance, quickly restore physical strength, and improve related physiological indicators in body fluids after activity;

⑷ It can increase blood sugar and serum testosterone levels in exercise-induced fatigue rats, prevent myocardial damage, and cause reproductive disorders in the human body; it can improve the body's ability to withstand hypoxia and has a therapeutic effect on diseases. Research has found that octacosanol reduces the risk of heart and brain tissue damage. Mild blood vessel dilation and cell edema have a certain protective effect on the structure of tissue cells and also have potential anti-inflammatory and anti-tumor effects;

⑸ A notable feature of octacosanol is that it has a very small dosage but significant physiological activity. It can quickly eliminate fatigue and enhance physical strength and endurance. It is a world-recognized anti-fatigue functional substance. According to reports from Nippon Oils and Fats Co., Ltd., usually 0.2 mg of octacosanol is taken orally per person per day to eliminate fatigue; in order to enhance physical strength and endurance, the daily oral dosage is about 0.5 mg; and for therapeutic purposes The dosage is 5mg;

⑹ Its safety is extremely high. Oral tests on mice have proven that the LD ₅₀ of octacosanol is over 18,000 mg/kg; its safety is much higher than that of table salt (LD ₅₀ = 3,000 mg/kg); clinical practice has proven that it can be taken for a long time without side effects.

⒉ Bamboo leaf flavonoids are a type of plant flavonoids, and the active ingredients are flavonoid glycosides and coumarin lactones. In addition to flavonoids, its components also include phenolic acids, anthraquinones, aromatic components, and trace elements such as manganese, zinc, and tin. Together, they form the basis for the extensive physiological and pharmacological activities of bamboo leaf flavonoids.

⑴ A study led by Dutch experts found that: An experiment with 4,807 participants showed that people who drank 375 ml of green tea a day had half the incidence of heart disease as those who did not drink tea; the incidence of fatal heart disease was Only a third. The important reason is the flavonoids contained in green tea (<<American Clinical Nutrition>> 2002.4.25);

Investigation and research on the physiological effects of bamboo leaf flavonoids showed:

① It has the functions of scavenging oxygen free radicals, resisting aging, preventing Alzheimer's disease, preventing vascular sclerosis, resisting vascular proliferation, resisting myocardial ischemia, lowering blood pressure, lowering blood lipids, and protecting cardiovascular and cerebrovascular diseases. It can be used to prevent and treat cardiovascular and cerebrovascular diseases. If it can reduce The fragility of blood vessels, dilating blood vessels and improving blood vessel permeability, preventing arteriosclerosis, lowering blood lipids and cholesterol, preventing and treating hypertension in the elderly, cerebral hemorrhage, coronary heart disease, angina pectoris, dilating coronary blood vessels, increasing coronary flow, lowering blood sugar, etc. good effect;

② It can induce the apoptosis of cancer cells and tumor cells, exert anti-cancer and anti-tumor effects, and delay the apoptosis of normal tissue cells;

③Radiation protection, liver protection, liver detoxification, treatment of acute and chronic hepatitis, liver cirrhosis, improvement of body immunity, diarrhea, cough relieving, expectoration, asthma, antispasmodic, anti-allergic, anti-allergic and anti-viral, etc. pharmacological effects;

⑷Almost all flavonoids have varying degrees of antibacterial activity against many microorganisms (including Gram-positive bacteria, Gram-negative bacteria and fungi);

⑤It can improve the body’s immune function and promote health;

⑥ It not only has many medicinal effects, but also has good stability. Because the structure contains phenolic hydroxyl groups, it is consistent with the acidic environment of human gastric juice and intestinal juice, which can avoid the first pass effect;

It has outstanding advantages in the following aspects:

①Stable structure and not easily degraded;

② It can penetrate deep into the lesion area and directly exert curative effect;

③ Increased hydrophilicity is beneficial to the development of drugs, food, cosmetics, etc.;

⑵Anti-fatigue

Oxidative stress has been shown to contribute to chronic fatigue through increased release of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and IL-1β;

Many studies have shown that flavonoid supplementation reduces muscle fatigue in fatigued rats. In the study of flavonoids, lutein-6-c-neohesperidin luteolin-6-c-neohesperidin (LN), isolated from moso bamboo, was tested in the forced swimming test of mice under weight. In the experiment, LN had an anti-fatigue effect on mice. Verified by a 3-week rat swimming model, LN can significantly extend the fatigue time of rats, thereby achieving an anti-fatigue effect;

⑶Anti-aging effect

There are about 70 kinds of human diseases, including cancer, cardiovascular and cerebrovascular diseases, all related to free radicals. Bamboo leaf flavonoids can effectively scavenge free radicals and produce antioxidant effects, thereby achieving the purpose of delaying aging;

⑷The effect of weight loss

Excess nutrition in the human body is converted into fat, and the fat stored in the body will be oxidatively denatured. This is one of the reasons why it is difficult for obese people to lose weight. Bioflavonoids can prevent lipid peroxidation, reduce fat accumulation, and become a new way to lose weight;

⑸It is a natural antioxidant

① It can significantly reduce the blood lactate content after exercise and enhance the activity of lactate dehydrogenase;

②The antioxidant performance is comparable to that of VE. As a new type of antioxidant, bamboo leaf flavonoids can prevent or delay the oxidation of food, improve the stability of food and extend the storage period;

⑹ It has antibacterial function and can be used in the food industry;

⑺Safe and non-toxic

Among its active ingredients, no toxic or harmful ingredients have been found so far, unlike Ginkgo biloba leaves which contain a certain amount of ginkgo acid, which is harmful to the human body;

The medicinal effects of bamboo leaves are recorded in ancient books such as "Compendium of Materia Medica" and "Medicine of Medicine" and the current "Dictionary of Traditional Chinese Medicine";

In 1998, (light) bamboo leaves were approved by the Ministry of Health to be included in the "list of natural plants for both medicine and food." It can be seen that bamboo leaves themselves are safe to use;

Research reports in recent years show that the acute toxicity test (Horn's method) was conducted on bamboo leaf flavonoids. According to the Ames test result judgment standard, the tested bamboo leaf flavonoids have no mutagenic effect;

In addition, the Zhejiang Academy of Medical Sciences conducted a toxicological safety evaluation on bamboo leaf flavonoid powder and bamboo leaf flavonoids from 1998 to 1999, confirming that bamboo leaf flavonoids have no toxic side effects and are highly safe.

⒊Development status, direction and focus

⑴Otacosanol

Since the current main raw materials for extracting octacosanol are sugarcane, bran wax, insecticide, and wheat germ oil, it is insoluble in water and soluble in organic solvents. In addition, the structure of the existing substance is relatively complex. Therefore, how to extract it from natural substances? Efficient, economical and environmentally friendly extraction is a problem that should be solved in the production of a large amount of high-quality such substances. At present, if the raw materials are saponified and extracted with organic solvents (n-hexane, acetone, etc.), a A white or light yellow powdery product. This powdery crystal is a natural high-grade alkanol mixture (containing higher alkanols with 24-34 carbon atoms). The content of octadecane in this mixture is 50-70%. , and the relevant pharmacological experiments in the data were also conducted on extracts with this content. At present, the product technology is only that the conventional extraction purity of octacosanol can only reach 40-80%, such as: a sports drink and its manufacturing method - 200510036559.0 (no rights - no annual fee paid) claim 5. The sports drink is characterized in that: the purity of the octacosanol used is greater than 80% . Such as: wheat germ beverage and its preparation method-201010615889.6 (no rights-unpaid annual fee) rights

Requirement 4. The described octacosanol is first extracted from wheat germ using a supercritical CO2 extraction method to obtain white flake crystals of octacosanol, in which the octacosanol content is 42.1 to 43.5% ;

⑵Traditional flavonoid extraction method

Flavonoids have very different solubility properties due to their different structures and sources. Therefore, appropriate solvents should be selected for extraction based on their polarity and water solubility. With the continuous deepening of research, there are more and more extraction methods for bamboo leaf flavonoids. According to the literature, the common extraction methods of bamboo flavonoids include hot water extraction, organic solvent extraction, microwave extraction, ultrasonic extraction, Supercritical fluid extraction, enzyme extraction, crushing extraction and other methods;

Experimental results show that microwave extraction of bamboo leaf flavonoids has the highest efficiency, followed by ultrasonic extraction, and water extraction has the worst efficiency, which is time-consuming and has low extraction rate. However, the stability of bamboo leaf flavonoids obtained by water extraction is relatively good;

The extracts obtained by the above-mentioned single methods have good stability in the pH range of 6 to 7. Conditions above 50°C and strong natural light are not conducive to the preservation and use of the extracts;

The single extraction rate of flavonoids by the above-mentioned single methods is not high, generally 40-60%, and requires 3 or more times, and even after 3 or more times of extraction, the purity is only 30-50%; such as ：A bamboo leaf flavonoid beverage and its preparation method 202010982466 Claim 15. A bamboo leaf flavonoid beverage, characterized in that it includes the following components by weight: moso bamboo leaf flavonoid extraction with a mass fraction of more than 50% 10 to 20 parts of liquid , 20 to 40 parts of bis-depleted apple juice, 3 to 5 parts of lemon juice concentrate, and 40 to 60 parts of water; the moso bamboo leaf flavonoid extract also contains moso bamboo leaf polysaccharide with a mass fraction of 5% to 7% ;

technical problem

In summary, although the extraction methods of octacosanol and bamboo leaf flavonoids have achieved certain results, they:

① At present, the above-mentioned purity in the market can only be used for anti-fatigue and improving human body function. If you want to achieve the effect of effectively lowering lipids and preventing cardiovascular and other diseases, the purity must reach at least 90%, thus limiting its use in food and With the further development of the pharmaceutical field, purity determines the physiological activity of a drug; it also brings a lot of trouble to further separation, and the subsequent purification process is relatively cumbersome and time-consuming;

② There are currently many types of functional beverages on the market that only add octacosanol or bamboo leaf flavonoids, but the single system method limits the scope of application and effect; there are still certain limitations in the industrial application of functional beverages.

⒋The present invention studies a high-energy beverage made by synergistically refining ultra-high-purity octacosanol-flavonoid compound deuterated water combination and its preparation method, which has strong application value.

At present, there are certain prior technological attempts to use bamboo leaf flavonoids or octacosanol alone in beverage processing technology. The excellent properties of bamboo leaf flavonoids or octacosanol themselves are self-evident, and their combined use There have been no relatively successful cases of this method so far; a high-energy drink made by synergistically refining ultra-high-purity octacosanol-flavonoid compound deuterated water combination and its preparation method have not been reported yet.

solution

Therefore, it is in line with people's expectations to provide a high-energy beverage that is synergistically refined with an ultra-high-purity octacosanol-flavonoid composite deuterated water combination and a preparation method thereof.

In order to achieve the above objects, the technical solutions adopted by the present invention are:

An ultra-high-purity octacosanol-flavonoid composite deuterated water combination synergistically refined high-energy drink and its preparation method, including the following components: 0.05 to 0.1 parts of octacosanol, 0.12 to 0.2 parts of flavonoids, and deuterated water. 900 to 1000 parts of water, 0.1 to 0.3 parts of stabilizer, 0.3 parts of compound emulsifier, 15 to 25 parts of water-soluble dietary fiber, 1.97 parts of compound electrolytes, 0.03 to 0.6 parts of preservatives, and 0.05 to 0.2 parts of sour agent;

The octacosanol is octacosanol with a purity of ≥98% extracted from fresh sugarcane filter mud;

The flavonoids are bamboo leaf flavonoids with a purity of ≥95%;

The detritiated water has a deuterium content of 50 to 100 ppm.

Preferably, the compound emulsifier is two or more of Tween 60, Tween 80, molecularly distilled monoglyceride, sucrose fatty acid ester, glyceryl monostearate, Span 60, and Span 80. A mixture of more than one species.

Preferably, the stabilizer is one or a mixture of two or more of gum arabic, fish gelatin, xanthan gum, pectin, sodium carboxymethyl cellulose, cyclodextrin and konjac gum.

Preferably, the compound electrolyte is a mixture of 0.65 parts of sodium citrate + 0.6 parts of calcium lactate + 0.37 parts of potassium chloride + 0.35 parts of magnesium lactate.

Preferably, the water-soluble dietary fiber is one or two of modified polydextrose, partially hydrolyzed guar gum (PHGG), wheat starch resistant dextrin, corn starch resistant dextrin, and fructooligosaccharides. mixture.

Preferably, the sour agent is one or a mixture of two of citric acid, malic acid, lactic acid and bird's nest acid.

Preferably, the preservative is one or a mixture of two of hydroxybenzoates, potassium sorbate, and nisin.

Preferably, the high-energy beverage made by synergistically refining the combination of ultra-high purity octacosanol-flavonoid composite deuterated water and its preparation method includes the following steps:

⑴Weigh 900 to 1000 parts of deuterated water and 15 to 25 parts of water-soluble dietary fiber according to the weight ratio and add them into the sealed reaction kettle. Start stirring at a speed of 300 to 500r/min. Stir until completely dissolved at room temperature. ; Add 0.05 to 0.1 parts of octacosanol, 0.12 to 0.2 parts of flavonoids, 0.1 to 0.3 parts of stabilizer, and 0.3 parts of compound emulsifier, and heat to 85°C at the same time to emulsify at a high shear speed of 1.8X104r/min. 25min; cool down to 60-70℃, and then use a homogenizer to homogenize twice with a pressure of 30-35Mpa. The first homogenization is 3 to 4 minutes with an interval of 25 minutes; the second homogenization is 1 to 2 minutes to obtain a stable base. material;

⑵Cool the temperature to 50-55°C, weigh 1.97 parts of the compound electrolyte, 0.03-0.6 parts of the preservative, and 0.05-0.2 parts of the sour agent according to the weight ratio and add the base material in step ⑴, and the rotation speed is 300-500r/min , continue stirring for 30 minutes;

⑶System sterilization: UHT, 105-121℃, 6-10 seconds;

⑷Filling: Obtain a high-energy drink that is synergistically refined with a combination of octacosanol-flavonoid compound deuterated water.

Preferably, the ultra-high-purity octacosanol-flavonoid compound deuterated water combination is a high-energy beverage that is synergistically refined. According to actual needs, by adjusting the changes in components, it is suitable for use in health care, beauty, weight loss, New generation sports drinks, astronauts, divers and military military high-energy drinks and other fields.

Preferably, the high-energy drink made by synergistically refining the ultra-high-purity octacosanol-flavonoid complex deuterated water combination:

⑴The materials selected do not contain mammalian animal ingredients, and there are no hidden concerns about zoonotic infectious diseases such as mad cow disease and foot-and-mouth disease. They are rich in sources and highly safe;

⑵Made from halal-certified raw materials, there is no religious distinction.

beneficial effects

⒈The first high-energy beverage to be synergistically refined using ultra-high purity octacosanol-flavonoid compound deuterated water combination

Nowadays, a kind of sports drink called "magic water" (also called high-energy drink) is quietly emerging in the world. It is based on the original sports drink and adds traditional Chinese medicine ingredients, so that it has outstanding effects. The original sports drink was a drink made by adding corresponding ingredients to the drink to replenish the water, sugar, salt, protein and vitamins that the human body loses during high-intensity exercise. Obviously, this is a passive supplement formulation concept;

From the perspective of modern medical theory, sports are an extreme physical activity. After strenuous exercise, the human body not only loses a large amount of the above-mentioned nutrients, but also has some functional overdraft of some organs due to the super-intense exercise, leaving them in extreme fatigue. Conditions that are difficult to recover quickly, such as the brain, heart, digestive system, and immune system. At this time, athletes will have poor sleep at night, rapid heartbeat, loss of appetite, poor gastrointestinal absorption function, and are susceptible to infectious diseases, etc.;

These, in turn, delay the full recovery of human body functions. This physical state cannot be solved by simply supplementing water, salt and other substances;

The present invention uses ultra-high purity octacosanol-flavonoid compound deuterated water combination to supplement some special nutrients and coordinate with refined high-energy drinks to regulate these organs in a state of extreme fatigue in a targeted manner:

⑴ The present invention adopts the company’s [a supercritical-microwave-assisted ultrafine homogenate negative pressure cavitation high pressure combination one-step preparation method to strengthen the extraction of total flavonoids from bamboo leaves (Patent application number: 202210302314.1)] and the purity is ≥95 % of bamboo leaf flavonoids, the analysis results of the main nutrients show that it contains flavonoids, active polysaccharides, phenolic acid compounds, anthraquinone compounds, manganese, zinc, selenium and other trace elements. Flavonoids have obvious physiological activities such as anti-ulcer, antispasmodic, antibacterial, anti-inflammatory, hypolipidemic, and analgesic effects; active polysaccharides are sugar chains bonded by glycosylated bonds, which are beneficial to the human body's esophagus, bladder, kidneys, lungs, It has curative effects on liver and uterine cancer, and can promote wound healing, lower blood pressure, detoxify, and relieve fatigue. The main mechanism is to enhance the body's liquid immunity and increase the activity of white blood cells and phagocytes; phenolic acid compounds can sterilize and make the body smell fragrant. It can refresh the brain, whiten the skin, clear away heat, relieve fire, nourish yin and nourish the skin. It can refresh the mind and reduce fatigue. Anthraquinone compounds have hemostatic, antibacterial, purgative and diuretic effects. Trace elements are one of the six indispensable nutrients for the human body. It has various health-care effects such as clearing fire and improving eyesight, moistening the lungs and relieving cough, laxative and detoxification, soothing alcohol and soothing the nerves, promoting cell metabolism, improving blood circulation, and enhancing human immunity. It can also treat bronchitis, hypertension, hyperlipidemia, diabetes, etc. Diseases are cured well;

Reflected in functional drinks:

①It is a natural antioxidant

It can significantly reduce the blood lactate content after exercise and enhance the activity of lactate dehydrogenase;

② Anti-aging effect

There are about 70 kinds of human diseases, including cancer, cardiovascular and cerebrovascular diseases, all related to free radicals. Bamboo leaf flavonoids can effectively scavenge free radicals and produce antioxidant effects, thereby achieving the purpose of delaying aging;

③The effect of weight loss

Excess nutrition in the human body is converted into fat, and the fat stored in the body will be oxidatively denatured. This is one of the reasons why it is difficult for obese people to lose weight. Bioflavonoids can prevent lipid peroxidation, reduce fat accumulation, and become a new way to lose weight;

④ Its versatility

In addition to its high anti-lipid oxidation properties, it is also a natural yellow pigment with antibacterial, bacteriostatic, deodorizing and flavoring effects;

The present invention has discovered and verified through experimental research the influence of the purity of bamboo leaf flavonoids on the concentration of high-density lipoprotein cholesterol in rat serum:

The dosage group of bamboo leaf flavonoids with a purity of 50% in the conventional market and the dosage group of bamboo leaf flavonoids with a purity of ≥95% are used in the present invention. The bamboo leaf flavonoids are melted with 1% gum arabic to prepare a corresponding dosage suspension, which is administered orally every day. once. Observe 5d. They were randomly divided into 2 groups according to body weight and weighed once a week. After 2 weeks, blood was collected from the eyeballs of rats without fasting to measure serum HDL-C;

Table 1 Effect of bamboo leaf flavonoids on serum HDL-C concentration in rats (average value)

At the beginning of the experiment, there was no significant difference in HDL-C concentration in the serum of each group; after 2 weeks of administration, the purity of the bamboo leaf flavonoids used in the present invention was ≥95%, which could significantly increase the serum HDL-C concentration of the test rats, and the conventional market purity 50% bamboo leaf flavonoids did not significantly increase serum HDL-C concentration (Table 1). Studies have shown that high-purity bamboo leaf flavonoids have the effect of increasing serum HDL-C concentration;

The experimental results provide the necessary theoretical basis for the development of bamboo leaf flavonoids lipid-lowering products.

⑵ The present invention uses the method described in our company’s [A preparation method for extracting octacosanol using an ultrasonic-microwave-assisted composite solvent secondary recrystallization and distillation synergistic refining combination (patent application number: 202210303916.9)] from fresh sugarcane filter mud. Octacosanol with a purity of ≥98% is extracted from it. It is a functional substance with anti-fatigue physiological activity. It has various physiological effects such as anti-fatigue, enhancing endurance and physical strength, improving myocardial function, and lowering blood lipids. It is a A very good natural health functional factor:

① It has the function of improving sports, such as enhancing endurance and physical strength, improving muscle strength, alleviating muscle pain, reducing muscle friction, and shortening muscle nerve reaction time;

② It has the effect of improving human physiological functions, mainly as follows: increasing energy (including response sensitivity, stress ability); improving response sensitivity; improving stress ability; strengthening heart function and oxygen transport capacity, reducing systolic blood pressure; improving The ratio of body metabolism, etc.;

③It can increase blood sugar and serum testosterone levels in bodies due to exercise fatigue and prevent myocardial damage;

④ It can improve the body's immune function, inhibit the biosynthesis of cholesterol, help the body's tolerance under hypoxic conditions, improve the oxygen transport capacity, and improve the body's working ability under plateau conditions.

⑤ Reduces mild blood vessel dilation and cell edema in heart and brain tissue, has a certain protective effect on the structure of tissue cells, and also has potential anti-inflammatory and anti-tumor effects;

⑥ Promote hormone effects and improve reproductive disorders in the human body;

⑦ Reduces excess calcium and fat content in blood, is a promoter of calcitonin formation, and can be used to prevent and treat hypercalcemia, osteoporosis, high cholesterol and high lipoprotein blood type diseases;

⑧Can be used to prevent and treat Parkinson’s disease in the elderly;

The present invention has discovered and verified through experimental research the influence of the purity of octacosanol on the biological effects of weightlessness in tail-suspended rats simulating weightlessness:

As we all know, a series of physiological and biochemical changes occur in astronauts' bodies under weightless conditions, such as increased blood viscosity, increased blood lipids, and loss of bone calcium;

In this experiment, rats were randomly divided into 3 groups: the tail suspension group (hereinafter referred to as group A), the tail suspension plus octacosanol with a purity of 80% in the conventional market (hereinafter referred to as group B), and the tail suspension plus the octacosanol used in the present invention. The octacosanol group with a purity of ≥98% (hereinafter referred to as group C); 8 rats in each group, the supply amount is 25 mg/kg body weight per rat per day, the experimental period is 14 days; on the end of the experiment, the rats are fasted for 12 hours Afterwards, blood and tissue samples were collected; heart blood was collected by cardiac puncture, trunk blood was collected after decapitation, the thymus and spleen were removed and weighed, and the left hind limb femur was separated and removed; the weight of immune organs and the fluidity of red blood cell membranes were observed. and biomechanical properties of femur, etc.:

⑴ Thymus and spleen weight

The weight of the thymus and spleen of rats in group B was significantly lower than that of rats in group A, but the weight of the thymus of rats in group C was 0.37g, which was significantly higher than that of rats in group B, 0.24g;

The results show that the octacosanol used in the present invention with a purity of ≥98% can inhibit thymus atrophy caused by suspended simulated weightlessness. The thymus and spleen are important immune organs of the body, which suggests that the purity used in the present invention is ≥98%. The octacosanol has a certain protective effect on the decline of immune function caused by suspension simulated weightlessness;

⑵Red blood cell membrane fluidity

The fluidity of the red blood cell membrane is usually expressed by the P value of the fluorescence polarization degree of the red blood cell membrane. The larger the P value, the smaller the hydrocarbon chain activity, the smaller the membrane fluidity, and the greater the blood microviscosity; conversely, the greater the membrane fluidity. The larger the blood, the smaller the microviscosity of the blood. After 14 days of suspension, the average P values of the red blood cell membranes of rats in groups B and C were 0.28 and 0.32 respectively, which were significantly higher than 0.26 in rats in group A. The viscosity of the blood of rats in group C was greater than that in group B. There is a downward trend compared with rats;

⑶Femoral biomechanical parameters

Table 2 Femoral three-point bending test mechanical parameters (average value)

Table 2 shows the biomechanical measurement values of the femur obtained through the three-point bending test. After 14 days of tail hanging, the maximum load and elastic limit load of the femur of rats in group C were significantly higher than those in groups A and B. The results show that the octacosanol used in the present invention with a purity of ≥98% can significantly resist the decline in the biomechanical properties of the femur of suspended rats;

Table 3 Biomechanical properties of rat femur (average)

Table 3 shows the biomechanical performance data of the femur calculated from the biomechanical and geometric parameters of the femur. The results show that the octacosanol group with a purity of ≥98% used in the present invention can significantly improve the biomechanics of the suspended rat femur. performance;

In summary, the experiments show that the octacosanol used in the present invention with a purity of ≥98% has the following functions on tail-suspended rats: ① significantly increase the weight of the thymus; ② improve the fluidity of the red blood cell membrane of simulated weightless rats; ③ significantly improve Femoral biomechanical properties; the octacosanol used in the present invention with a purity of ≥98% can effectively combat certain adverse effects of tail-suspended rats; during the entire experimental process, no side effects of octacosanol were observed; It shows that a series of physiological and biochemical changes that occur in the body of astronauts under the condition of weightlessness can be used to improve exercise endurance by taking health drinks or foods added with octacosanol with a purity of ≥98% used in the present invention. , reduce blood lipids, improve blood viscosity, improve hypoxic endurance and prevent osteoporosis, and may become a health drink or food ingredient for astronauts.

⒊The deuterated water used in the present invention has a deuterium content of 25 to 100 ppm, and is used in disease prevention and drinking water.

In nature, the deuterium content is 1/7000 of hydrogen. The deuterium content in natural water is generally 145-150ppm, the deuterium content in seawater is 155ppm, and the deuterium content in glacier water is about 138-140ppm. In fact, deuterated water is water that uses modern technology to reduce deuterium to 10-130ppm;

As more and more high-carbohydrate foods enter the body, coupled with unhealthy lifestyles such as often staying up late, drinking late night snacks, eating and drinking, and long-term lack of exercise, there are too many harmful substances in the cells of modern people, which is " deuterium". Deuterium has an accumulative effect in the body and is difficult to metabolize on its own. It can cause many adverse effects on the human body's DNA, heredity, metabolism and enzyme system;

There have been many studies abroad on the benefits of drinking deuterated water, proving the biological effects of deuterated water. This is a major breakthrough in the application of deuterated water in the fields of nuclear medicine and water physiology abroad in recent years:

⑴Research from the Japan Deuterated Water Research Association shows that a deuterated water environment can hinder the metabolism of cancer cells and induce their death. At the same time, drugs and the body's immunity will also be effective in attacking cancer cells;

⑵ An experiment by the DDCENTER Deuterium Removal Research Center in the United States found that drinking deuterium-removed water can increase resting metabolic rate and relieve fatigue;

⑶In the book "Cancer Prevention and Treatment - Biological Effects of Deuterated Water" written by American expert Professor LÁSZLÓ G. BOROS, the significance of deuterium in regulating biological metabolism was demonstrated, and the role of deuterated water in tumor prevention and treatment was demonstrated in clinical trials. It has also been found that: a low deuterium environment has a positive impact on the treatment process of diabetes, helping patients better control blood sugar levels;

⑷Hungary's HYD Cancer Research and Drug Development Institute has obtained ethical approval for the phase 2 clinical trial of deuterated water and found that deuterated water can be adapted to be used as an auxiliary product for other tumors such as prostate cancer, breast cancer, lung cancer, and pancreatic cancer;

⑸A research report on the anti-aging effect of deuterated water was published in "Toxicology Letters" in 2012. The results showed that deuterated water reversed the shortening of lifespan caused by manganese (Mn) exposure and restored the effect of extending lifespan in nematodes. The levels of DAF-16 and SOD-3 play an important role;

⑹ In 2016, "Behavioral Brain Research" published a research report that high deuterium may increase the incidence of depression. The study found that deuterated water increased the EEG index of awakening and REM sleep duration, and eliminated typical depressive state behaviors. , deuterated water may provide new prevention strategies for depression;

For example, in Hunza, a famous longevity village in the Caucasus, almost no one suffers from cancer, heart disease, abnormal blood pressure and other common diseases among modern people. The investigation found that the deuterium content in local daily drinking water and crop irrigation was lower than 133ppm, which was far lower than the 150ppm in plain areas and the 155ppm in equatorial areas;

For people on a ketogenic diet or those who want to lose weight and get in shape, drinking deuterated water is also an option that can help achieve results.

Using deuterated water to make non-alcoholic beverages, such as drinking water, mineralized water, magnetized water, soft drinks and functional drinks, can improve human health and quality of life. At present, the United States, Russia, Hungary, Ukraine, Japan, Germany, France, etc. have deuterated water products on the market. Low-deuterium water has been used in various metabolic diseases and cancers;

In short, water is the source of life. The content of deuterium in water is an important criterion for measuring the quality of water. Deuterated water is not only used in scientific research. Deuterated water can activate immune cells, improve the body's basal metabolic level, resist cell mutation and delay Functions such as aging are more beneficial to the survival, development and reproduction of living organisms, and are of great significance to human health.

⒋This invention uses water-soluble dietary fiber, an alternative and innovative solution for reducing sugar and replacing sugar.

According to Nielsen data, among the five product types that Generation Z has paid more for in the past year, low-fat/low-calorie/low-sugar products rank first. Generation Z believes that food and beverages should not be a burden on the body, and they want to eat healthier;

Since the explosion of zero-sugar sparkling water, we have seen the concept of sugar reduction begin to rapidly expand to bread, dairy products, chocolate candies and other categories. However, although sweeteners can help reduce sugar intake, they still sacrifice product taste to a certain extent. How can we reduce sugar healthily without sacrificing taste? This will be an issue that brands will have to address when creating sugar-reduced products in the future;

⑴This invention uses water-soluble dietary fiber technology to replace sugar.

The water-soluble dietary fiber used in the present invention is not only a high-quality water-soluble dietary fiber, but also a unique low-calorie filler; sugar is replaced with dietary fiber to reduce carbohydrate content and calories, and the plant-based one-to-one applied Sugar substitute; not only is it a good source of natural fiber, but it does not use any masking agents and has no aftertaste, giving it the same flavor as regular sugar;

⑵ According to the preferences and perceptions of different consumers, adjusting the plan can balance and enhance the overall flavor and taste experience; Table 4

①PHGG can significantly reduce the incidence of nosocomial influenza

Since the new coronavirus ravaged the world at the end of 2019, humans have been fighting this "ferocious influenza virus" for three years. Various studies on how to prevent "flu" and new coronary pneumonia caused by the new coronavirus have emerged one after another. In order to effectively control the new coronavirus epidemic, Provide academic reference;

The latest Japanese single-center retrospective cohort study article The effect of partially hydrolyzed guar gum on preventing influenza infection was published in April 2021 to explore the effect of PHGG in preventing influenza infection. ;The results show that PHGG can affect the intestinal microenvironment, thereby helping to reduce the incidence of influenza;

The clinical benefits brought by PHGG are still under further study. Current studies have found that in addition to improving intestinal health, it can also improve blood sugar, lower cholesterol and blood lipids, enhance mineral absorption, etc. It has been used as a substitute in 45 countries Fern sugar and fat are used to produce low-calorie, low-sugar, low-fat, low-cholesterol and low-salt foods. In Asia, it is used as a fiber ingredient to add to fiber-fortified beverages.

②Resistant dextrin

Has excellent physiological functions:

(1) Helps maintain normal and healthy blood sugar and insulin levels, delays and inhibits the digestion and absorption of sugars in the small intestine, improves the sensitivity of peripheral tissues to insulin, and reduces the need for insulin;

Relevant studies have shown that daily intake of a certain amount of resistant dextrin can effectively prevent the symptoms of diabetes;

(ii) Change the secretion of digestive tract hormones and the activity of digestive enzymes in the intestine, inhibit the digestion and absorption of sugar, thereby lowering blood sugar. Continuous intake of this low molecular weight water-soluble dietary fiber can reduce serum cholesterol and neutral fat concentrations and body fat mass;

(iii) It can adsorb bile acids, fats, etc. to reduce their absorption rate, which can lower blood lipids and improve lipid metabolism in patients with various types of hyperlipidemia;

(4) It is not absorbed in the small intestine and can directly enter the large intestine. It can promote the growth and reproduction of beneficial intestinal flora while inhibiting the growth and reproduction of harmful intestinal microorganisms. It is fermented in the large intestine to produce short-chain fatty acids, and the amount of acid produced is higher than that of the same diet. Rich in fiber, these short-chain fatty acids can prevent the growth and reproduction of cancer cells;

(5) Water absorption and expansion can increase the volume of feces, promote intestinal peristalsis, and have a good preventive effect on constipation, hemorrhoids, colon cancer and other diseases;

(VI) Helps prevent dental caries;

So far, resistant dextrins have been used in the soup products of the U.S. Soup Company, Danone Company of France, Nestlé and other soups, beverages and dairy products. The largest beverage production companies, Coca-Cola Company and Pepsi-Cola Company, have also used resistant dextrin. This material is also used in the production of health drinks;

Resistant dextrin is recognized by the US FDA as a GRAS substance and has no intake limit. In Japan, resistant dextrin is widely used as a nutritional fortifier and has been recognized as a government-specified health food material by the Japanese Ministry of Health, Labor and Welfare. In my country, the Ministry of Health [2012] Letter No. 516 plans to announce that resistant dextrin is a common food and can be added to various foods in unlimited amounts;

③Modified polydextrose

Only partially participates in the metabolism of the human body, and the heat it generates is only IkCal/g (4.18kJ/g); when it acts as a filler to replace other sugars or fats in food raw materials, the calories provided are 25 times that of sugars. %, 11% of fat. Moreover, after being ingested into the human body, its metabolic process has nothing to do with insulin, so it will not cause changes in blood sugar and is suitable for diabetic patients.

According to the results of a clinical study conducted by Tomotari Mistuoka of the University of Tokyo on some healthy volunteers who took 10g of polydextrose daily, it was shown that polydextrose can prevent the reabsorption of these steroids; Decreased enzyme activity indicates that polydextrose can reduce the risk of colon cancer;

In Japan, modified polydextrose is considered an important source of dietary fiber. Research shows that when young women drink a bottle of beverage containing 7-10g of modified polydextrose every day for 5 consecutive days, their feces will become significantly softer and constipation will be effectively relieved;

In some countries such as China, Japan and South Korea, modified polydextrose is regarded as an important water-soluble dietary fiber;

④Fructooligosaccharides

It can effectively reduce the amount of serum cholesterol, triglycerides, and free fatty acids, and has a good effect on improving a series of cardiovascular diseases such as hypertension and arteriosclerosis caused by high blood lipids. Fructooligosaccharides are fermented by bacteria in the large intestine to generate L-lactic acid, which can dissolve calcium, magnesium, iron and other minerals and promote the body's absorption of minerals. It inhibits the growth of harmful bacteria and regulates the balance in the intestine; it can promote the absorption and utilization of trace elements iron and calcium to prevent osteoporosis; it can reduce liver toxins and generate anti-cancer organic acids in the intestine, which has significant anti-cancer effects. Cancer function; and the taste is pure, sweet and delicious, with a fat-like aroma and a refreshing smoothness. Low caloric value. Since fructo-oligosaccharide cannot be directly digested and absorbed by the human body and can only be absorbed and utilized by intestinal bacteria, it has low caloric value and will not cause obesity. It also has an indirect weight loss effect. Also a good sweetener for diabetics;

Experiments have confirmed that fructooligosaccharide promotes calcium absorption by 70.8%. Therefore, fructooligosaccharides can promote growth and development and prevent osteoporosis; at the same time, it can also promote the natural formation of vitamins B1, B2, B3, B6, B12 and folic acid, thereby improving the body's metabolism, immunity and disease resistance. Prevent and improve skin diseases caused by toxins in the body, prevent facial sores, dark spots, freckles, acne, and age spots, make the skin bright and slow down aging. After Bifidobacterium absorbs fructooligosaccharides, it rapidly proliferates, inhibits the action of spoilage bacteria such as Escherichia coli, Salmonella and Clostridium, reduces the production of toxic metabolites (such as indole, nitrosamine), and rapidly metabolizes the toxicity oligosaccharides are excreted from the body, reduce the burden on the liver, protect the liver, and prevent various chronic diseases, cancers, etc.; fructo-oligosaccharides are rarely decomposed by gastric acid and enzymes in the digestive tract, and are extremely difficult to be absorbed by the human body;

According to the measurement, the caloric value of fructo-oligosaccharide is less than 1.5Kcal/g, while the caloric value of sucrose is 4.6Kcal/g. Therefore, after ingesting fructo-oligosaccharide, it will not cause obesity and is an ideal low-calorie functional sweetener. . Fructooligosaccharides cannot be used by Streptococcus mutans to generate insoluble glucan and provide a place for oral microbial deposition, acid production and corrosion (tartar), thus preventing dental caries.

⒌The alternative emulsification and stabilization solution adopted by this invention

The present invention has discovered and verified through experimental research: The influence of various factors on the stability of a high-energy beverage collaboratively refined with an ultra-high-purity octacosanol-flavonoid compound deuterium-free water combination:

⑴The impact of emulsifiers on beverage stability

The selection of emulsifier type and dosage plays an important role in the formation of a stable emulsion system; a suitable emulsifier can significantly reduce the surface tension between the two phases, change the interface state of the system, and help stabilize the uniform dispersion of droplets. ;

①Test on beverage stability of single-factor oil-soluble emulsifier

The oil-soluble emulsifier Span 80 was selected as an example to conduct experiments to examine the stabilizing effect of its dosage on beverages;

Table 5 Results of the effect of oil-soluble emulsifiers on beverage stability

②Test on the stability of beverages using single-factor water-soluble emulsifiers

As a water-soluble emulsifier, Tween 80 was selected as an example to conduct experiments to examine the stabilizing effect of its dosage on beverages;

Table 6 Results of the effect of water-soluble emulsifiers on beverage stability

③The impact of compound emulsifiers on beverage stability

In order to improve the emulsification effect, different levels of combinations of the oil-soluble emulsifier Span 80 (A) and the water-soluble emulsifier Tween 80 (B) were selected to conduct dual-factor repeated tests as an example. The concentration of A was 0.05 parts, There are three levels of 0.1 part and 0.15 part, and the concentration of B has three levels of 0.10 part, 0.15 part and 0.2 part. Taking the centrifugal sedimentation rate (%) as the test standard, the smaller the centrifugal sedimentation rate, the better. The test results are shown in Table 3:

Table 7 Double-factor and other repeated test data and calculation table

The results in Table 7 show that when the optimal combination level is 0.2 parts of Tween 80 and 0.1 part of Span 80, the centrifugal sedimentation rate of the beverage is the smallest. Therefore, the beverage made from the above combination formula can achieve the best emulsification;

⑵The impact of stabilizers on beverage stability

①Single factor test of multiple stabilizers

This experiment selected three commonly used hydrophilic colloids: xanthan gum, sodium carboxymethyl cellulose (CMC), and gum arabic as stabilizers to conduct a single-factor test on body stability. Under the same conditions, the beverage was observed to change from static to static. When a large amount of precipitation occurs, the type of stabilizer required for the final test will be determined based on the stabilizing effect;

Table 8 Single factor test of various stabilizers

It can be seen from Table 8 that xanthan gum, sodium carboxymethyl cellulose (CMC), and gum arabic have better effects when used alone in beverages. Among them, xanthan gum has the best effect, which is in line with its own growth rate. Characteristics of the strongest thickening effect;

②The impact of several stabilizer combinations on beverage stability

In the single stabilizer comparison test, various stabilizers did not achieve the desired effect. Therefore, this experiment further used composite stabilizers for testing. According to the single factor test results, xanthan gum (0.4 parts, 0.6 parts, 0.8 parts), carboxymethyl cellulose sodium CMC (0.2 parts, 0.4 parts, 0.6 parts), arabic gum (0.2 parts, 0.4 parts, 0.6 parts) ) These three factors are the influencing factors of stabilizers. An orthogonal test is conducted, and the optimal ratio and dosage are determined using the centrifugal sedimentation rate as an indicator. When measuring the precipitation rate, each treatment was repeated three times and the average value was taken. The experimental design results are shown in Table 9

Table 9 Determination of orthogonal test results for compound stabilizers

It can be seen from Table 9 that when the optimal combination of stabilizers is 0.6 parts of xanthan gum, 0.2 parts of sodium carboxymethylcellulose CMC, and 0.4 parts of gum arabic, the centrifugal sedimentation rate of the beverage is the smallest; therefore, the beverage made with the above combination formula The beverage can achieve the best stability;

Xanthan gum serves as a thickener, emulsifier, and stabilizer. Due to its pseudoplasticity, xanthan gum can increase the richness of the drink and stabilize the suspension of each component. It is thickened but not sticky, and has good fragrance release; As an emulsification aid in beverages, gum arabic has good water solubility and emulsification as a food adhesive. During the embedding process, it can increase the efficiency of the embedding, enhance the taste, prevent precipitation and maintain the flavor of the capsule; carboxyl Sodium methylcellulose (CMC) has multiple functions such as thickening, suspension, emulsification, and stabilization. It has good water retention, dispersion, and thixotropy, and can control the size and growth of ice crystals during transportation and storage. Used as a protective agent for beverages; therefore, the combination of the above three can prevent beverages from agglomerating;

⑶The impact of other factors on beverage stability

①The impact of high-speed shearing conditions on beverage stability

The impact of high shear emulsification speed on beverage stability is also significant. Tests show that when the stirring speed is very low, the emulsification effect of the beverage is poor, and there is obvious floating and stratification phenomenon. As the stirring speed continues to increase, the stability of the beverage shows a proportional increase trend, but when the emulsification speed reaches 1.8x104r/ After min, the growth gradually slows down;

Based on the above factors, 1.8x104r/min is selected as the optimal emulsification speed; the emulsification time is too short, the emulsification process is not sufficient, and the floating phenomenon of the drink is serious; when the emulsification process is basically completed, extending the stirring time will have minimal improvement in stability. , has little value in actual production, so the emulsification time is 25 minutes;

②The impact of homogenization conditions on beverage stability

Homogenization is a common physical method to prevent precipitation and stratification of beverages. The choice of homogenization temperature and pressure directly affects the homogenization effect. In this test, the pressure is 30-35Mpa and the temperature is 60-70℃ to homogenize the beverage. The test shows that when the pressure is 30-35Mpa and the temperature is 60-70℃, there is no stratification of the product when homogenized twice. , floating and settling phenomena. It can also be seen from the experiment that the greater the pressure, the higher the temperature, the more times of homogenization, and the finer the particles, but the temperature and pressure are too high. Too many times of homogenization will consume too much production cost. Therefore, the pressure is 30-35Mpa, the temperature is 60-70°C, and the homogenization is performed twice. The first homogenization is 3 to 4 minutes with an interval of 25 minutes; the second homogenization is 1 to 2 minutes. After the electron microscope inspection, it is obvious that the diameter is 3 μm. ~5μm oil-in-water droplets. It can produce drinks with good quality, good texture and stable structure;

The order of priority of the impact of various factors on beverage stability is: high shear emulsification time > water-soluble emulsifier dosage > oil-soluble emulsifier dosage > stabilizer dosage;

③Influence on product stability

(1) System sterilization

UHT, 105-121℃, 6-10 seconds; can achieve better sterilization effect without causing obvious changes in beverage properties; however, exceeding or using a long-term heating method for sterilization will cause the solute to stratify and float. ;

(ii) Storage stability

Three storage conditions of 37°C, 4°C, and normal temperature were used respectively. The storage time at 37°C and 4°C was 2 weeks, and the storage time at room temperature was 30 days. The experimental results showed that there was no floating or sedimentation, and there was no obvious change in the taste, indicating that the beverage technology The formula is stable and has good storage stability.

⑸ “Subversion” of high-energy beverage structure

Breaking the routine is the direct way of technological innovation. Through insight into consumer needs and innovative changes to the high-energy beverage structure, it not only brings new health solutions, but also enhances consumers’ experience:

① [A preparation method for extracting octacosanol using a combination of ultrasonic-microwave-assisted composite solvent secondary recrystallization and distillation synergistic refining (Patent Application Number: 202210303916.9)] The purity extracted from fresh sugarcane filter mud is ≥ The purity of 98% octacosanol and [a supercritical-microwave-assisted ultrafine homogenate negative pressure cavitation and high pressure combination one-step enhanced extraction of total flavonoids from bamboo leaves (patent application number: 202210302314.1)] is ≥95% The bamboo leaf flavonoids are all invented and owned by our company, with similar ultra-high purity and technology. This aspect has not been reported yet;

Based on the common knowledge in the industry, purity determines the physiological activity of a drug. The purity of flavonoids and octacosanol must reach at least 90% to achieve the effects of effectively lowering lipids and preventing cardiovascular and other diseases. The purity used in the present invention is ≥ 95% bamboo leaf flavonoids and octacosanol with a purity of ≥98% can be used in beverages not only for anti-fatigue and improving human body function, but also for further development in fields such as disease prevention;

The present invention proposes a synergistic combination of ultra-high-purity octacosanol-flavonoid complex deuterium-free water, which can be reasonably and effectively strengthened and improved by cross-comparison and integration based on the characteristics of each extract. It regulates complementary and synergistic effects, which is beneficial to absorption and circulation in the body; it introduces ultra-high purity extracts into beverages, changing the concept of passive supplementation and creating the concept of actively conditioning beverages. It has more superior effects in weight loss and other functions, further expanding its development in fields such as disease prevention; according to actual needs, by adjusting the changes in components, it can be used for health care, new generation sports drinks, divers, astronauts and military development military supplies In the fields of high-energy drinks and other fields, this aspect has not yet been reported; it is not only refreshing, but also provides theoretical reference for better development of high-energy drinks. It will become the production concept of high-energy drinks in the future, and will attract more and more attention from the society; it is important for enriching China's new It is of great practical significance to create a new generation of functional high-energy drinks and promote the development of human health; therefore, the present invention will have extremely broad and practical value;

② The present invention solves the problem of poor water solubility of octacosanol and flavonoids. It will not cause any flavor and sensory problems in the beverage and has good uniformity;

③This invention uses water-soluble dietary fiber to reduce sugar and replace sugar as an alternative and innovative solution;

④The materials selected are made from halal certified raw materials, without religious distinction; sugar-free, pigment-free, green, pure and healthy, which also provides quality and safety guarantee for halal high-energy drinks;

⑤The refining process of the present invention is relatively simple and time-saving.

The present invention will be further described below in conjunction with the examples: The technical solutions provided by the present invention will be described in detail below in conjunction with the examples, but they should not be understood as limiting the protection scope of the present invention.

Description of the drawings

Figure 1 shows the purity test report of bamboo leaf flavonoids extracted from this product.

Figure 2 shows the results of the purity test report of bamboo leaf flavonoids extracted from this product.

Figure 3 is the purity test report of octacosanol extracted from fresh sugarcane filter mud by this product.

Figure 4 shows the purity test report results of this product extracting octacosanol from fresh sugarcane filter mud.

Best Mode of Carrying Out the Invention Example

An ultra-high-purity octacosanol-flavonoid composite deuterated water combination synergistically refined high-energy drink and its preparation method, including the following components: 0.05 to 0.1 parts of octacosanol, 0.12 to 0.2 parts of flavonoids, and deuterated water. 900 to 1000 parts of water, 0.1 to 0.3 parts of stabilizer, 0.3 parts of compound emulsifier, 15 to 25 parts of water-soluble dietary fiber, 1.97 parts of compound electrolytes, 0.03 to 0.6 parts of preservatives, and 0.05 to 0.2 parts of sour agent;

The octacosanol is octacosanol with a purity of ≥98% extracted from fresh sugarcane filter mud;

The flavonoids are bamboo leaf flavonoids with a purity of ≥95%;

The deuterated water has a deuterium content of 25 to 100 ppm.

Its preparation method includes the following steps:

⑴Weigh 100 ppm of deuterated water, 1000 parts of water-soluble dietary fiber, and 15 parts of corn starch-resistant dextrin according to the proportion by weight, and add them into the sealed reaction kettle, start stirring at a speed of 500 r/min, and stir until completely dissolved at room temperature. ;Add 0.1 part of octacosanol, 0.2 part of flavonoids, 0.6 part of xanthan gum as stabilizer, 0.2 part of sodium carboxymethylcellulose CMC, 0.4 part of gum arabic, 0.2 part of compound emulsifier Tween 80, 0.1 part of Si Pan 80, heat to 85℃ at the same time, emulsify for 25min at a high shear speed of 1.8x104r/min; cool to 70℃, then use a homogenizer to homogenize twice with a pressure of 35Mpa, the first homogenization is 4min, and the interval 25min; second homogenization for 2min to obtain a stable base material;

⑵Cool the temperature to 50-55℃, weigh 1.97 parts of the compound electrolyte, 0.25 parts of the preservative hydroxybenzoates, and 0.2 parts of the sour agent citric acid according to the weight ratio and add it to the base material in step ⑴, and the rotation speed is 500r/min , continue stirring for 30 minutes;

⑶System sterilization: UHT, 105-121℃, 10 seconds;

⑷Filling: Obtain a high-energy beverage that is synergistically refined with an ultra-high purity octacosanol-flavonoid compound deuterated water combination.

Embodiments of the invention Example

An ultra-high-purity octacosanol-flavonoid composite deuterated water combination synergistically refined high-energy drink and its preparation method, including the following components: 0.05 to 0.1 parts of octacosanol, 0.12 to 0.2 parts of flavonoids, and deuterated water. 900 to 1000 parts of water, 0.1 to 0.3 parts of stabilizer, 0.3 parts of compound emulsifier, 15 to 25 parts of water-soluble dietary fiber, 1.97 parts of compound electrolytes, 0.03 to 0.6 parts of preservatives, and 0.05 to 0.2 parts of sour agent;

The octacosanol is octacosanol with a purity of ≥98% extracted from fresh sugarcane filter mud;

The flavonoids are bamboo leaf flavonoids with a purity of ≥95%;

The deuterated water has a deuterium content of 25 to 100 ppm.

Its preparation method includes the following steps:

⑴Weigh 950 parts of 50ppm deuterated water and 20 parts of partially hydrolyzed guar gum (PHGG) in water-soluble dietary fiber according to the proportion by weight, add them to the sealed reaction kettle, start stirring, the speed is 400r/min, stir at room temperature until After completely dissolved; add 0.05 parts of octacosanol, 0.2 parts of flavonoids, 0.17 parts of fish gelatin as stabilizer, 0.03 parts of konjac gum, 0.15 parts of compound emulsifiers sucrose fatty acid ester, 0.1 part of glyceryl monostearate, and at the same time Heat to 85℃, emulsify for 25min at a high shear speed of 1.8x104r/min; cool to 65℃, and then use a homogenizer with a pressure of 33Mpa to homogenize twice, the first time for 3.5min, with an interval of 25min; Homogenize twice for 1.5 minutes to obtain a stable base material;

⑵Cool the temperature to 50-55°C, weigh 1.97 parts of the compound electrolyte, 0.3 parts of the preservative potassium sorbate, and 0.1 part of the sour agent malic acid according to the weight ratio as the base material in step ⑴, and the rotation speed is 400r/min. Continue stirring for 30 minutes;

⑶System sterilization: UHT, 105-121℃, 8 seconds;

⑷Filling: Obtain a high-energy beverage that is synergistically refined with an ultra-high purity octacosanol-flavonoid compound deuterated water combination.

Example

An ultra-high-purity octacosanol-flavonoid composite deuterated water combination synergistically refined high-energy drink and its preparation method, including the following components: 0.05 to 0.1 parts of octacosanol, 0.12 to 0.2 parts of flavonoids, and deuterated water. 900 to 1000 parts of water, 0.1 to 0.3 parts of stabilizer, 0.3 parts of compound emulsifier, 15 to 25 parts of water-soluble dietary fiber, 1.97 parts of compound electrolytes, 0.03 to 0.6 parts of preservatives, and 0.05 to 0.2 parts of sour agent;

The octacosanol is octacosanol with a purity of ≥98% extracted from fresh sugarcane filter mud;

The flavonoids are bamboo leaf flavonoids with a purity of ≥95%;

The deuterated water has a deuterium content of 25 to 100 ppm.

Its preparation method includes the following steps:

⑴ Weigh 900 parts of 25 ppm deuterated water and 25 parts of modified polydextrose of water-soluble dietary fiber according to the proportion by weight and add them into the sealed reaction kettle. Start stirring at a speed of 300r/min. Stir until completely dissolved at room temperature; add 0.15 parts of octacosanol, 0.12 parts of flavonoids, 0.3 parts of stabilizer, gum arabic, 0.045 parts of compound emulsifier, molecularly distilled monoglyceride, 0.045 parts of sucrose fatty acid ester, 0.18 parts of Tween60, heated to 85°C at the same time, at 1.8 Emulsify for 25 minutes at a high shear speed of base material;

⑵Cool the temperature to 50-55°C, weigh 1.97 parts of the compound electrolyte, 0.03 parts of the preservative nisin, and 0.05 parts of the sour agent lactic acid according to the weight ratio and add it to the base material in step ⑴. The rotation speed is 300r/min. Continue Stir for 30 minutes;

⑶System sterilization: UHT, 105-121℃, 6 seconds;

⑷Filling: Obtain a high-energy beverage that is synergistically refined with an ultra-high purity octacosanol-flavonoid compound deuterated water combination.

Comparative Example 1-1

An ultra-high-purity octacosanol-flavonoid composite deuterated water combination synergistically refined high-energy drink and its preparation method, including the following components: 0.05 to 0.1 parts of octacosanol, 0.12 to 0.2 parts of flavonoids, and deuterated water. 900 to 1000 parts of water, 0.1 to 0.3 parts of stabilizer, 0.3 parts of compound emulsifier, 15 to 25 parts of water-soluble dietary fiber, 1.97 parts of compound electrolytes, 0.03 to 0.6 parts of preservatives, and 0.05 to 0.2 parts of sour agent;

The octacosanol is octacosanol with a purity of ≥98% extracted from fresh sugarcane filter mud;

The flavonoids are bamboo leaf flavonoids with a purity of ≥20%;

The deuterated water has a deuterium content of 25 to 100 ppm.

Its preparation method includes the following steps:

⑴Weigh 100 parts per million of deuterated water and 15 parts of corn starch-resistant dextrin according to the proportion by weight and add them into the sealed reaction kettle. Start stirring at a speed of 500 r/min. Stir until completely dissolved at room temperature. ;Add 0.1 part of octacosanol, 0.2 part of flavonoids, 0.6 part of xanthan gum as stabilizer, 0.2 part of sodium carboxymethylcellulose CMC, 0.4 part of gum arabic, 0.2 part of compound emulsifier Tween 80, 0.1 part of Si Pan 80, heat to 85℃ at the same time, emulsify for 25min at a high shear speed of 1.8x104r/min; cool to 70℃, then use a homogenizer to homogenize twice with a pressure of 35Mpa, the first homogenization is 4min, and the interval 25min; second homogenization for 2min to obtain a stable base material;

⑵Cool the temperature to 50-55℃, weigh 1.97 parts of the compound electrolyte, 0.25 parts of the preservative hydroxybenzoates, and 0.2 parts of the sour agent citric acid according to the weight ratio and add it to the base material in step ⑴, and the rotation speed is 500r/min , continue stirring for 30 minutes;

⑶System sterilization: UHT, 105-121℃, 10 seconds;

⑷Filling: Obtain a high-energy beverage that is synergistically refined with an ultra-high purity octacosanol-flavonoid compound deuterated water combination.

Comparative Example 1-2

An ultra-high-purity octacosanol-flavonoid composite deuterated water combination synergistically refined high-energy drink and its preparation method, including the following components: 0.05 to 0.1 parts of octacosanol, 0.12 to 0.2 parts of flavonoids, and deuterated water. 900 to 1000 parts of water, 0.1 to 0.3 parts of stabilizer, 0.3 parts of compound emulsifier, 15 to 25 parts of water-soluble dietary fiber, 1.97 parts of compound electrolytes, 0.03 to 0.6 parts of preservatives, and 0.05 to 0.2 parts of sour agent;

The octacosanol is octacosanol with a purity of ≥80% extracted from fresh sugar cane filter mud;

The flavonoids are bamboo leaf flavonoids with a purity of ≥95%;

The deuterated water has a deuterium content of 25 to 100 ppm.

Its preparation method includes the following steps:

⑴Weigh 100 parts per million of deuterated water and 15 parts of corn starch-resistant dextrin according to the proportion by weight and add them into the sealed reaction kettle. Start stirring at a speed of 500 r/min. Stir until completely dissolved at room temperature. ;Add 0.1 part of octacosanol, 0.2 part of flavonoids, 0.6 part of xanthan gum as stabilizer, 0.2 part of sodium carboxymethylcellulose CMC, 0.4 part of gum arabic, 0.2 part of compound emulsifier Tween 80, 0.1 part of Si Pan 80, heat to 85℃ at the same time, emulsify for 25min at a high shear speed of 1.8x104r/min; cool to 70℃, then use a homogenizer to homogenize twice with a pressure of 35Mpa, the first homogenization is 4min, and the interval 25min; second homogenization for 2min to obtain a stable base material;

⑵Cool the temperature to 50-55℃, weigh 1.97 parts of the compound electrolyte, 0.25 parts of the preservative hydroxybenzoates, and 0.2 parts of the sour agent citric acid according to the weight ratio and add it to the base material in step ⑴, and the rotation speed is 500r/min , continue stirring for 30 minutes;

⑶System sterilization: UHT, 105-121℃, 10 seconds;

⑷Filling: Obtain a high-energy beverage that is synergistically refined with an ultra-high purity octacosanol-flavonoid compound deuterated water combination.

Comparative experiment 1

In order to observe the effect of high-energy drinks on the concentration of serum high-density lipoprotein cholesterol in rats, a comparative experiment was carried out between Example 1 and Control Example 1-1, and the serum high-density lipoprotein cholesterol (HDL-C) level index of rats was measured. , giving specific data reference for the effect of high-energy drinks on increasing density lipoprotein cholesterol (HDL-C):

Rats were selected, provided with basic feed, and had free access to water and food; the room temperature in the breeding room was 23±5°C, the relative humidity was 40% to 70%, and the lighting changed naturally. After the animals were observed and adapted for 5 days, 80 animals were selected for experiments and randomly divided into 4 groups. Three of the groups were fed mixed high-fat animal model feed: 20% sucrose, 15% lard, 1.2% cholesterol, 0.2% sodium cholate, 6.6% casein, 0.5% calcium hydrogen phosphate, 0.3% stone powder, and the drinking water of two of the three groups of mice was replaced with the high-energy drink prepared in Example 1 and Comparative Example 1-1. of high-energy drinks. Weigh once a week, record food intake and calculate food utilization. The trial lasts for 30 days. In the middle period of the experiment, blood was collected from the eyeballs without fasting, and serum HDL-C was measured. At the end of the experiment, blood was collected from the eyeballs of the rats without fasting, and HDL-C in the serum was measured. After dissection and observation, the liver, spleen, kidney, testis, perirenal fat and peritesticular fat were collected and weighed, and the corresponding indexes were calculated. SPSS16.0 software was used to statistically analyze the data. Data are expressed as mean ± standard error (∑ ± s), significance test uses one-way analysis of variance, and comparison between data uses t test method. The result is as follows:

Table 10 Effect on serum LDL-C concentration in rats

Judging from the results, when the bamboo leaf flavonoids used in the present invention have a purity of ≥95%, the serum HDL-C concentration of the test rats can be significantly increased. When the bamboo leaf flavonoids with a purity of 20% are used, the serum HDL-C concentration is not significantly increased ( Table 10). Research shows that when the present invention adds high-purity bamboo leaf flavonoids, it has the effect of significantly increasing serum HDL-C concentration.

Comparative experiment 2

In order to observe the effects of different purity extracts of high-energy drinks on improving the swimming time and anti-fatigue effects of mice, a comparative experiment was carried out between Example 1 and Control Examples 1-2. 30 rats were taken and randomly divided into Example 1 Prepare beverages (hereinafter referred to as beverage group A), prepare beverages in Comparative Examples 1-2 (hereinafter referred to as beverage group B), deionized water blank control group (hereinafter referred to as group C), 10 mice in each group, and administer intragastric administration to mice , 10ml each, the dosage is equivalent to 3g/kg, administered continuously for 15 days and 30min after the last dose:

①Pole climbing test and weight-bearing swimming test

The trained and screened mice were placed on the upper end of a vertically suspended smooth glass rod, so that their muscles were in a state of static tension. The mice were recorded from climbing the rod until their muscles were fatigued and unable to hold the glass rod and fell to the ground. Time, repeat 3 times, terminate the test after the third fall, and the cumulative time of 3 times is used as the pole climbing time (s);

Each group continued to be administered intragastrically for 5 days. 30 minutes after the last administration, 8% of the body weight was weighted with a lead wire at the base of its tail. The mice were placed in a swimming box at room temperature, and the time from the beginning of swimming to the death of the mice was recorded. Swimming time (min);

Deionized water blank control group experiment: Mice were given deionized water by gavage, 10 ml each, at a dose equivalent to 3g/kg, for 5 consecutive days, and then performed weight-bearing swimming 30 minutes after the last administration, in room temperature water. Put 8% of the body weight on the tail with a lead skin, swim until exhaustion, sink into the water and stay up for 10 seconds, record the time;

The actual test results on the impact on exercise endurance are as follows:

Table 11 Effect of each test group on mouse endurance (average value)

②Influence on biochemical indicators after swimming

On the 15th day of intragastric administration, 30 minutes after administration, a weight of 3% of the mouse's body weight was placed at the base of the tail with a lead wire, and the mice were taken out after swimming in room temperature water for 30 minutes. The weight was removed, and blood was collected from the supraorbital venous plexus after resting for 15 minutes. Determination of blood lactate content by p-hydroxybiphenyl chromogenic method;

Mice in each group were administered intragastrically for 5 days. 30 minutes after the last administration, they were put into normal temperature water for 60 minutes and then taken out. Blood was taken from the supraorbital venous plexus and serum urea nitrogen was measured using a kit method. Then the animals were killed immediately, the liver was removed, rinsed with 0.9% NaCl solution, dried with filter paper, and the liver glycogen content was measured by the anthrone method;

②Result

Table 12 Effect of octacosanol extract on some biochemical indicators of swimming mice (average value)

After mice were gavaged for 15 days and swam under weight, the blood lactate in group A was significantly lower than that in group B, indicating that this effect may be related to the purity of the test substance;

The liver glycogen content of group A was significantly higher than that of group B, indicating that the octacosanol used in the present invention with a purity of ≥98% can improve the glycogen accumulation capacity in animals;

The serum urea nitrogen level was measured after swimming in mice administered intragastric administration for 20 days. The level of serum urea nitrogen in group A was significantly lower than that in group B. The difference was extremely significant, indicating that the octacosanol used in the present invention with a purity of ≥98% can significantly reduce the serum urea nitrogen level. Urea nitrogen, improves the body's endurance and adaptability to exercise load;

From the above test, it can be seen that when the beverage group A uses octacosanol with a purity of ≥98%, it has a significant anti-fatigue effect, indicating that the high-energy beverage of the present invention has an anti-fatigue health care effect; the beverage group B uses It is low-purity octacosanol, and its anti-fatigue effect is significantly lower than that of beverage group A.

All the above embodiments are only used to illustrate the technical solutions of the present invention and do not constitute a limitation on the implementation. Although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the above-mentioned embodiments. The technical solutions described in each embodiment are modified, or some of the technical features are equivalently replaced; these modifications or substitutions, as well as other conceivable alternatives, are all within the protection scope of the present invention and do not make the corresponding technical solutions The essence deviates from the spirit and scope of the technical solutions of each embodiment of the present invention.

Industrial applicability

Conventional technical knowledge and industry consensus in this field, the current domestic and foreign research on the extraction technology status of bamboo leaf flavonoids:

A method for preparing bamboo leaf flavonoid extract through column purification 200910223926.6 shows that the purity of bamboo leaf flavonoids is 20.78%;

A production method for extracting bamboo leaf flavonoids from bamboo leaves 200610053134.5 shows that the purity of bamboo leaf flavonoids is ≥24%;

A method for separating and purifying flavonoids and lactones from bamboo leaves based on microwave-assisted technology CN201510481490 shows that the purity of bamboo leaf flavonoids is ≥29%;

A preparation method of bamboo leaf flavonoids extract 200910223927.0 shows that the purity of bamboo leaf flavonoids is 42%;

A method for extracting flavonoids from bamboo leaves 201110003352.9 shows that the purity of bamboo leaf flavonoids is 30~50%;

A method for extracting bamboo leaf flavonoids 201110406495.4 shows that the purity of bamboo leaf flavonoids is 30~50%;

The production process for extracting bamboo leaf flavonoid series products from bamboo leaves 200610053436.2 shows that the purity of bamboo leaf flavonoids is 60%;

The preparation method of bamboo leaf flavonoids extract 200810195064.6 shows that the purity of bamboo leaf flavonoids is 40%-65%;

Through experimental optimization, You Hui et al. obtained the appropriate purification process conditions as solution pH 5.0, adsorption flow rate, then washing the column with 3BV water, and then eluting with 4BV 60% ethanol solution at a flow rate of 1.5mL-min. It was determined that the flavonoid content in the purified product was 54.16%;

Zhu Hongli et al. extracted the crude bamboo leaf extract with chloroform, and then passed the chloroform layer sample through X-5 macroporous resin. After elution with a high-concentration alcohol solution, the primary purified sample was passed through a polyamide column. The total flavonoid content in the purified sample can reach 78.97%;

Taiyuan University of Technology - Research Progress on Flavonoids in Bamboo Leaves - The article shows: Bamboo leaf flavonoid powder with a flavonoid content of 24% was obtained;

Central South University of Forestry and Technology - Research on Extraction, Purification and Antioxidant Activity of Bamboo Leaf Flavonoids - The article shows that the total flavonoid content in the obtained product is 36%;

School of Food Science and Engineering, Yangzhou University-Research on physiological activities and applications of bamboo leaf flavonoids-

The article shows that the flavonoid content of the obtained bamboo leaves reaches more than 40%;

Department of Food, School of Biological Science and Technology, Beijing Forestry University - Research on the optimization and analysis characteristics of adsorbents in the purification of bamboo leaf flavonoids - the article shows: the purity of bamboo leaf flavonoids produced by this process reaches 54.16%;

Nanchang University - Pilot-scale extraction of active polysaccharides and flavonoids from moso bamboo leaves - the article shows that the purity of flavonoids from moso bamboo leaves after being refined with AB-8 resin reaches 79.4%;

Therefore, according to the conventional technical knowledge in this field and industry consensus, the bamboo leaf flavonoids described in Reference Document 2 are only a conceptual summary, and are actually just a crude extract with low extraction rate and many impurities (such as phenols, Saponics, whiplash, alkaloids, polysaccharides, protein, crude fat, chlorophyll, anthraquinone compounds, mushroom lactosides, inorganic salts, moisture, minerals, ash, heavy metals, arsenic and manganese, zinc , selenium and other trace elements, more than 20 mineral elements, 82 aromatic compounds, 16 amino acids, 53 volatile components, 10 alcohols, 17 aldehydes, etc.), the so-called bamboo leaf flavonoids are only the components of these substances Some of the contents are, at best, just a trade name.

According to the conventional technical knowledge and industry consensus in this field, the current status of extraction technology of octacosanol is studied at home and abroad:

Jin Baoyuan and others studied the process of separating and preparing octacosanol from Changbai Mountain beeswax, but the equipment investment was large, the process was complex, and the yield was low, making it difficult to industrialize production.

Zhang Xiangnian et al. melted the insect wax, poured it into ether, and used the reduction method to catalyze it with LiAIH4. After reduction at 70-80°C for 2.5 hours, a high-carbon fatty alcohol mixture was obtained. After purification by molecular distillation, a mixture containing 16.7% octacosanol was obtained. Small quantity of product.

Chen Fang et al. used transesterification reaction to extract higher fatty alcohols from bran wax. The results showed that the yield of octacosanol was 43.99% and the content of octacosanol was 10.98%. After molecular distillation of the mixed alcohol, 28 The alkanol content can reach 37.6%.

Song Jianhua et al. used mixed solvents to extract wax from insect wax, with a yield of up to 9% and a content of octacosanol of 41.3%.

Wang Xingguo et al. used crude bran wax as raw material, refined it, and used ultrasonic hydrolysis of rice bran wax, extraction of fatty alcohols, and molecular distillation technology to separate octacosanol and triacontanol to prepare products with a purity greater than 80%.

Xiamen University Chemical Plant uses Sichuan and other beeswax as raw materials, and uses the residue from the production of triacontanol to extract octacosanol. The purity is 12.8% and 18%. However, due to raw materials and cost reasons, it is very competitive in terms of output and price. reduce.

In addition, Xiamen University also uses insecticide wax as raw material to produce octacosanol. The outstanding disadvantage is that the cost is high, and the content of octacosanol has not made a big breakthrough.

The article [Preparation and Application of High-Purity Octacosanol] from the School of Environmental and Chemical Engineering of Nanchang University describes: Using wormwood wax as raw material, long carbon chain fatty alcohols are prepared by alcohol phase saponification method, and high purity is obtained by recrystallization. For octacosanol, capillary gas chromatography was used to measure the octacosanol content in the product. The results showed that the average yield of long-chain fatty alcohol was 23.30% and the octacosanol content was 39.08%.

The literature of Nanchang University [Research on the Preparation, Purification and Application of Octacosanol] describes: The preparation of octacosanol from beeswax has a low yield and low purity; the yield of octacosanol from insect wax is as high as 9%, and the higher alcohol content reaches 97.1%, of which the content of octacosanol is 41.3%.

Jiangnan University Oubaite Food Co., Ltd. has developed a product with a octacosanol content of 50 to 60% using rice bran wax and has passed the pilot test. However, due to the impact of the market, the current production is not large. Other current domestic octacosanol manufacturers are shown in Table 13

Table 13 Domestic octacosanol manufacturers

 

Wheat germ beverage and its preparation method - 201010615889.6 The octacosanol described in claim 4 is first extracted from the wheat germ using a supercritical CO2 extraction method to obtain white flake crystals of octacosanol, of which 28 Alkanol content is 42.1~43.5%;

Domestic production of octacosanol mostly uses rice bran wax as raw material. The purity of octacosanol in the product is low and it is a low-end product.

The 24-34 carbon higher alkanols contained in Cuban Policosanol account for more than 90% of the weight of the extract, of which the octacosanol content is 60%-70%.

In Japan, most octacosanol is extracted from rice bran oil and rice germ oil. The content of octacosanol is generally 10%~15%, which is a mixture of C22~C36 fatty alcohols. Most of the octacosanol produced by octacosanol is made from rice bran oil and rice germ oil. The octacosanol content in octacosanol products is usually 12%.

At present, the content of octacosanol purified by most international and domestic manufacturers is generally about 12%, which is a mixture of C22~C36 fatty alcohols. Even Japanese octacosanol products only contain about 15%. The early products of octacosanol were mainly wheat germ oil concentrates; currently, except for Japan’s progress in using rice bran to produce octacosanol, there is no large-scale production technology of octacosanol from rice bran abroad. No advantage. Since the current main raw materials for extracting octacosanol are rice bran wax, wormwood, wheat germ oil, and sugar cane wax, because it is insoluble in water and soluble in organic solvents, and the structure of the existing substance is relatively complex, currently such substances are If the raw material is saponified and extracted with an organic solvent (n-hexane, acetone, etc.), a white or light yellow powdery body will be obtained. This powdery crystal is a natural high-grade alkanol mixture (containing 24-34 carbon atoms). Higher alkanols), the current product technology only contains less than 50% octadecane. The conventional extraction purity of octacosanol can only reach 15-35%. At present, foreign products are generally C22~C36 fatty alcohol mixtures. The octacosanol content is generally 10%~15%. The highest content reported in China is 60%, so currently 98% polyol and 60% octacosanol have become the best-selling specifications on the market.

Therefore, according to the conventional technical knowledge in this field and industry consensus, the bamboo leaf flavonoids described in Reference Document 2 are only a conceptual summary, and are actually just a crude extract with low extraction rate and many impurities (such as phenols, Saponics, whiplash, alkaloids, polysaccharides, protein, crude fat, chlorophyll, anthraquinone compounds, mushroom lactosides, inorganic salts, moisture, minerals, ash, heavy metals, arsenic and manganese, zinc , selenium and other trace elements, more than 20 mineral elements, 82 aromatic compounds, 16 amino acids, 53 volatile components, 10 alcohols, 17 aldehydes, etc.), the so-called bamboo leaf flavonoids are only the components of these substances Some of the contents are, at best, just a trade name.

Bamboo leaf flavonoids and octacosanol of different purity have different effects in different fields. Because the higher the purity of bamboo leaf flavonoids and octacosanol, the stronger their activity and greater polarity. If you want to effectively lower lipids, prevent cardiovascular and other diseases, improve hypoxic endurance and prevent osteoporosis, For performance and other effects, the purity must reach at least 90%. Therefore, purity determines the physiological activity of a drug;

In summary, although the current methods for extracting flavonoids and octacosanol from bamboo leaves have achieved certain results, the purity of the flavonoids and octacosanol products produced by current manufacturers is relatively low and poses problems for further separation. A lot of trouble, and the subsequent refining process is relatively cumbersome and time-consuming; the above-mentioned purity in the market can only be used for anti-fatigue and improving human body function, thus limiting its further development in the food and pharmaceutical fields;

Therefore, how to extract efficient, economical and environmentally friendly natural substances is a problem that should be solved in production to obtain a large amount of high-quality such substances. Therefore, how to prepare high-purity flavonoids and octacosanol at low cost is currently the focus of research by scientists from various countries.

The octacosanol used in the present invention is extracted from fresh sugar cane filter mud with a purity of ≥98%; the flavonoids used are bamboo leaf flavonoids with a purity of ≥95%. According to the characteristics of each extract, crossover and integration are carried out, and ultra-high purity extracts are introduced into beverages, which changes the concept of passive supplementation and creates the concept of actively conditioning beverages, which reasonably and effectively greatly strengthens and improves the complementary and synergistic effects of beverages on body regulation. It has more superior effects in anti-fatigue, improving human body functions, health care, beauty and weight loss, etc., further expanding its development in disease prevention and other fields; according to actual needs, by adjusting the changes in components, it can be used for health care , new generation sports drinks, astronauts and military development of military high-energy drinks and other fields.

Claims (8)

1. An ultra-high-purity octacosanol-flavonoid composite deuterated water combination synergistically refined high-energy drink and its preparation method, which is characterized by: including the following components: 0.05 to 0.1 parts of octacosanol and 0.12 to 0.2 parts of flavonoids. 900 to 1000 parts of deuterated water, 0.1 to 0.3 parts of stabilizer, 0.3 parts of compound emulsifier, 15 to 25 parts of water-soluble dietary fiber, 1.97 parts of compound electrolytes, 0.03 to 0.6 parts of preservatives, and 0.05 parts of sour agent ~0.2 parts;

   The octacosanol is octacosanol with a purity of ≥98% extracted from fresh sugarcane filter mud;

   The flavonoids are bamboo leaf flavonoids with a purity of ≥95%;

   The deuterated water has a deuterium content of 25 to 100 ppm.
2. An ultra-high-purity octacosanol-flavonoid composite deuterated water combination collaboratively refined high-energy beverage and its preparation method according to claim 1, characterized in that: the compound emulsifier is Tween 60, A mixture of two or more of Tween 80, sucrose fatty acid ester, molecularly distilled monoglyceride, glyceryl monostearate, Span 60, and Span 80.
3. An ultra-high-purity octacosanol-flavonoid composite deuterated water combination collaboratively refined high-energy beverage and its preparation method according to claim 1, characterized in that: the stabilizer is gum arabic, fish gelatin, One or a mixture of two or more of xanthan gum, pectin, sodium carboxymethylcellulose CMC, cyclodextrin, and konjac gum.
4. An ultra-high-purity octacosanol-flavonoid composite deuterated water combination collaboratively refined high-energy drink and its preparation method according to claim 1, characterized in that: the compound electrolyte is 0.65 parts of sodium citrate A mixture of + 0.6 parts of calcium lactate + 0.37 parts of potassium chloride + 0.35 parts of magnesium lactate.
5. An ultra-high-purity octacosanol-flavonoid composite deuterated water combination collaboratively refined high-energy beverage and its preparation method according to claim 1, characterized in that: the water-soluble dietary fiber is modified polydextrose , one or a mixture of two of partially hydrolyzed guar gum (PHGG), wheat starch resistant dextrin, corn starch resistant dextrin, and fructooligosaccharides.
6. An ultra-high-purity octacosanol-flavonoid composite deuterated water combination collaboratively refined high-energy beverage and its preparation method according to claim 4, characterized in that: the sour agent is citric acid, malic acid, One or a mixture of both lactic acid and bird's nest acid.
7. An ultra-high-purity octacosanol-flavonoid composite deuterated water combination collaboratively refined high-energy drink and its preparation method according to claim 1, characterized in that: the preservative is hydroxybenzoate, One or a mixture of two of potassium sorbate and nisin.
8. Used to prepare a high-energy beverage that is synergistically refined with an ultra-high purity octacosanol-flavonoid composite deuterated water combination as claimed in claim 1 and its preparation method, which is characterized in that it includes the following steps:

   ⑴Weigh 900 to 1000 parts of deuterated water and 15 to 25 parts of water-soluble dietary fiber according to the weight ratio and add them into the sealed reaction kettle. Start stirring at a speed of 300 to 500r/min. Stir until completely dissolved at room temperature. ; Add 0.05 to 0.1 parts of octacosanol, 0.12 to 0.2 parts of flavonoids, 0.1 to 0.3 parts of stabilizer, and 0.3 parts of compound emulsifier, and heat to 85°C at the same time to emulsify at a high shear speed of 1.8X104r/min. 25min; cool down to 60-70℃, and then use a homogenizer to homogenize twice with a pressure of 30-35Mpa. The first homogenization is 3 to 4 minutes with an interval of 25 minutes; the second homogenization is 1 to 2 minutes to obtain a stable base. material;

   ⑵Cool the temperature to 50-55°C, weigh 1.97 parts of the compound electrolyte, 0.03-0.6 parts of the preservative, and 0.05-0.2 parts of the sour agent according to the weight ratio and add the base material in step ⑴, and the rotation speed is 300-500r/min , continue stirring for 30 minutes;

   ⑶System sterilization: UHT, 105-121℃, 6-10 seconds;

   ⑷Filling: Obtain a high-energy beverage that is synergistically refined with an ultra-high purity octacosanol-flavonoid compound deuterated water combination.