WO2023208768A1 - Procédé de production de distillat de cannabis - Google Patents
Procédé de production de distillat de cannabis Download PDFInfo
- Publication number
- WO2023208768A1 WO2023208768A1 PCT/EP2023/060432 EP2023060432W WO2023208768A1 WO 2023208768 A1 WO2023208768 A1 WO 2023208768A1 EP 2023060432 W EP2023060432 W EP 2023060432W WO 2023208768 A1 WO2023208768 A1 WO 2023208768A1
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- WO
- WIPO (PCT)
- Prior art keywords
- cannabis
- distillate
- enzyme
- oleoresin
- phospholipase
- Prior art date
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- 235000019420 glucose oxidase Nutrition 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000010460 hemp oil Substances 0.000 description 1
- 108010030923 hesperidinase Proteins 0.000 description 1
- 235000011167 hydrochloric acid Nutrition 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 108010090785 inulinase Proteins 0.000 description 1
- 239000001573 invertase Substances 0.000 description 1
- 235000011073 invertase Nutrition 0.000 description 1
- 229940057428 lactoperoxidase Drugs 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 239000001630 malic acid Substances 0.000 description 1
- 235000011090 malic acid Nutrition 0.000 description 1
- DJMVHSOAUQHPSN-UHFFFAOYSA-N malto-hexaose Natural products OC1C(O)C(OC(C(O)CO)C(O)C(O)C=O)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(OC4C(C(O)C(O)C(CO)O4)O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 DJMVHSOAUQHPSN-UHFFFAOYSA-N 0.000 description 1
- FJCUPROCOFFUSR-UHFFFAOYSA-N malto-pentaose Natural products OC1C(O)C(OC(C(O)CO)C(O)C(O)C=O)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 FJCUPROCOFFUSR-UHFFFAOYSA-N 0.000 description 1
- FJCUPROCOFFUSR-GMMZZHHDSA-N maltopentaose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O[C@H]([C@H](O)CO)[C@H](O)[C@@H](O)C=O)O[C@H](CO)[C@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O[C@@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O)[C@@H](CO)O2)O)[C@@H](CO)O1 FJCUPROCOFFUSR-GMMZZHHDSA-N 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229940051921 muramidase Drugs 0.000 description 1
- 229940055695 pancreatin Drugs 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 229940072417 peroxidase Drugs 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- YHHSONZFOIEMCP-UHFFFAOYSA-O phosphocholine Chemical compound C[N+](C)(C)CCOP(O)(O)=O YHHSONZFOIEMCP-UHFFFAOYSA-O 0.000 description 1
- 229950004354 phosphorylcholine Drugs 0.000 description 1
- 229940085127 phytase Drugs 0.000 description 1
- 108010004131 poly(beta-D-mannuronate) lyase Proteins 0.000 description 1
- 108010039928 polymethylgalacturonase Proteins 0.000 description 1
- 235000013809 polyvinylpolypyrrolidone Nutrition 0.000 description 1
- 229920000523 polyvinylpolypyrrolidone Polymers 0.000 description 1
- 229920006316 polyvinylpyrrolidine Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 108060006633 protein kinase Proteins 0.000 description 1
- 230000000506 psychotropic effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 108010038851 tannase Proteins 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 239000011345 viscous material Substances 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
- B01D11/0288—Applications, solvents
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
- B01D11/028—Flow sheets
- B01D11/0284—Multistage extraction
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
- B01D11/0292—Treatment of the solvent
Definitions
- CN107027903A mentions enzymatic degumming of cannabis oil where phospholipase is part of the process but the type of phospholipid and substrate cannabis oil are not defined and thus the efficiency of the process and purity of the cannabis oil product are unknown. Additionally, references in the document to free fatty acids and solid lipids indicate that the cannabis oil in this document refers to the triglyceride oil obtained from the seeds of the hemp plant. Further, the minor amounts of water used in the description of the degumming process in this document indicate that the product involved is a triglyceride oil product and not a solvent extracted oleoresin.
- enzyme refers to any one or combination of multiple enzymes.
- the enzyme(s) can include cellulases, hemi-cellulases, pectinases, lipases, phospholipases, and combinations thereof.
- a phospholipase C may be an enzyme from enzyme classification number EC 3.1 .4.3 that cleaves phospholipids between the phosphate and the glycerol group, resulting in a diglyceride and a phosphate compound such as choline phosphate or ethanolamine phosphate.
- a PLC is for instance known from W02005/086900, WO2012/062817 or WO2016/162456.
- the cannabis extract can undergo a filtration step prior to the extraction solvent removal step.
- This filtration step is another distinction in the process as compared to seed oil processes where filtration prior to enzymatic treatments is not used.
- Conventional filter media such as, but not limited to, bleaching clay, activated charcoal, polyvinylpyrolladone (PVPP), and combinations thereof. Any conventional filtration equipment can used such as filter presses, filter columns, etc. Those of skill in the art will be familiar with such filtration equipment.
- the wash solvent removal step can involve typical equipment and operations as those of skill in the art will be familiar.
- the cannabis distillate is placed in a rotary evaporator for the wash solvent removal step.
- the rotary evaporator is operated with an amount of vacuum at a wash solvent removal temperature.
- the amount of vacuum can be from about 20 inches of mercury (inHg) to about 30 InHg.
- the wash solvent removal temperature can range from about 60°C to about 80°C.
- Solvent Wash a Hexane was added to the cannabis pre-distillate along with water in a ratio of about 37 parts cannabis pre-distillate to 13 parts water. b. The material was mixed and the water drained from the bottom of the mixing vessel. c. The solvent wash was repeated 8 times.
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
La présente invention concerne des procédés de production de distillats de cannabis à l'aide d'enzymes pour traiter l'oléorésine de cannabis.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202263334700P | 2022-04-26 | 2022-04-26 | |
| US63/334,700 | 2022-04-26 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2023208768A1 true WO2023208768A1 (fr) | 2023-11-02 |
Family
ID=86328744
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/EP2023/060432 WO2023208768A1 (fr) | 2022-04-26 | 2023-04-21 | Procédé de production de distillat de cannabis |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO2023208768A1 (fr) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005086900A2 (fr) | 2004-03-08 | 2005-09-22 | Diversa Corporation | Phospholipases, acides nucleiques les codant et procedes de fabrication et d'utilisation associes |
| WO2011046812A1 (fr) | 2009-10-16 | 2011-04-21 | Verenium Corporation | Phospholipases, acides nucléiques codant pour celles-ci et leurs procédés de fabrication et d'utilisation |
| WO2012062817A1 (fr) | 2010-11-12 | 2012-05-18 | Novozymes A/S | Polypeptides ayant une activité phospholipase c et polynucléotides codant pour ceux-ci |
| WO2016162456A1 (fr) | 2015-04-09 | 2016-10-13 | Dsm Ip Assets B.V. | Phospholipase c |
| CN107027903A (zh) | 2017-05-16 | 2017-08-11 | 广西巴马印象生活体验产业有限公司 | 一种火麻油的精炼工艺 |
| WO2020028198A1 (fr) * | 2018-08-03 | 2020-02-06 | Biomass Oil Separation Solutions, Llc | Procédés et appareil d'extraction de substances et d'extraits enrichis à partir d'une matière végétale |
| CN110981703A (zh) * | 2019-12-20 | 2020-04-10 | 黑龙江阳光工业大麻研究院 | 一种大麻二酚的纯化方法 |
| US10973864B2 (en) | 2017-01-14 | 2021-04-13 | Herbolea Biotech S.R.L. | Enzyme-assisted lipid-based extraction and stabilization of phyto-cannabinoids and terpens and products obtained thereof |
-
2023
- 2023-04-21 WO PCT/EP2023/060432 patent/WO2023208768A1/fr unknown
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005086900A2 (fr) | 2004-03-08 | 2005-09-22 | Diversa Corporation | Phospholipases, acides nucleiques les codant et procedes de fabrication et d'utilisation associes |
| WO2011046812A1 (fr) | 2009-10-16 | 2011-04-21 | Verenium Corporation | Phospholipases, acides nucléiques codant pour celles-ci et leurs procédés de fabrication et d'utilisation |
| WO2012062817A1 (fr) | 2010-11-12 | 2012-05-18 | Novozymes A/S | Polypeptides ayant une activité phospholipase c et polynucléotides codant pour ceux-ci |
| WO2016162456A1 (fr) | 2015-04-09 | 2016-10-13 | Dsm Ip Assets B.V. | Phospholipase c |
| US10973864B2 (en) | 2017-01-14 | 2021-04-13 | Herbolea Biotech S.R.L. | Enzyme-assisted lipid-based extraction and stabilization of phyto-cannabinoids and terpens and products obtained thereof |
| CN107027903A (zh) | 2017-05-16 | 2017-08-11 | 广西巴马印象生活体验产业有限公司 | 一种火麻油的精炼工艺 |
| WO2020028198A1 (fr) * | 2018-08-03 | 2020-02-06 | Biomass Oil Separation Solutions, Llc | Procédés et appareil d'extraction de substances et d'extraits enrichis à partir d'une matière végétale |
| CN110981703A (zh) * | 2019-12-20 | 2020-04-10 | 黑龙江阳光工业大麻研究院 | 一种大麻二酚的纯化方法 |
Non-Patent Citations (2)
| Title |
|---|
| GODFREY, TWEST S: "Industrial enzymology", 1996, STOCKTON PRESS, pages: 299 - 300 |
| HIRSCHBERG HJ ET AL., EUR J BIOCHEM, vol. 268, no. 19, 2001, pages 5037 - 44 |
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