WO2023197586A1 - Device and method for monitoring virus in aerosol in real time by using discharge spectral imaging - Google Patents

Abstract

A detection device and method for monitoring virus in an aerosol in real time by using discharge spectral imaging. In the detection device: a Tesla high-frequency high-voltage discharger (1) with a discharge electrode (2) is inserted into a metal container (3); a high-light-transmittance circular slide (4), an air inlet and an air outlet are provided on the metal container (3), the air inlet is connected to an aerosol sample injector (8,9) and the air outlet is connected to an aerosol aftertreatment device (11); an electric filter lens turntable (5), an imaging lens (6) and a photon imaging or detection device (7) are provided above the high-light-transmittance circular slide (4). By means of the imaging lens (6), the electric filter lens turntable (5) and the high-transmittance circular slide (4), the photon imaging or detection device (7) uses the discharge electrode (2) to induce the weak light radiation of the aerosol sample in the metal container (3), thereby realizing imaging detection. The detection device is highly sensitive and rapid, has good operability and scalability, can realize long-distance unmanned control and real-time monitoring, and can be widely applied to various scenarios related to the monitoring of human and animal pathogenic virus.

Description

Device and method for real-time monitoring of viruses in aerosols using discharge spectrum imaging Technical field

The invention relates to a system technology that uses Tesla coil high-frequency and high-voltage discharge to act on aerosol samples, causing changes in weak light radiation to detect whether the aerosol contains viruses. The detection device and method constructed in the present invention are applied in the fields of medicine, health, biomedicine, biomedical engineering and biophotonics.

Background technique

For a long time, pathogenic viruses transmitted through aerosols have posed a serious threat to human and animal health, especially the emergence of unknown viruses. It is usually difficult to predict and monitor in advance before causing obvious disease in humans and animals, because it needs to be recovered from infected animals. Biological samples are obtained from humans or animals, and then nucleic acids, specific antigens, and antibodies are detected and analyzed using existing technologies. This traditional detection process often results in a delayed time window for virus monitoring. Therefore, it is very necessary to develop new technologies that can predict, screen and monitor unknown or existing viruses in the air and in the exhaled breath of humans and animals in real time.

Contents of the invention

The object of the present invention is to provide a device and method for real-time monitoring of viruses in aerosols using discharge spectrum imaging. The present invention performs real-time detection of viruses in aerosols based on Tesla discharge spectrum imaging and has the characteristics of sensitivity, speed, operability and good expandability. , can realize the advantages of unmanned remote control and real-time monitoring, and can be widely used in various scenarios involving the monitoring of human and animal pathogenic viruses, such as human life and public activity venues, customs, airports, schools and hospitals, as well as animal Breeding and processing sites, etc.

A discharge spectrum imaging real-time monitoring device for detecting viruses in aerosols, including a Tesla high-frequency and high-voltage discharger, a metal container, a highly transparent circular glass slide, an electric filter turntable, an imaging lens, a photon imaging or detection device, Aerosol injector and aerosol post-processing device, characterized in that: the discharge electrode of the Tesla high-frequency and high-voltage discharger is inserted into a metal container, and the metal container is provided with a highly transparent circular glass slide, an air inlet and an outlet. The air inlet is connected to the aerosol injector, and the air outlet is connected to the aerosol post-processing device; there is an electric filter turntable, imaging lens, photon imaging or detection device, and photon imaging above the highly transparent circular glass slide. Or the detection device realizes imaging or detection of weak light radiation of the aerosol sample in the metal container induced by the discharge electrode through the imaging lens, the electric filter turntable and the highly transparent circular glass slide.

The invention also includes an air pump, which is connected to the air inlet of the metal container, and the air pump cleans the air or aerosol sample in the container.

Use Tesla coils for high-frequency and high-voltage discharge, use discharge electrodes to induce weak light radiation from aerosol virus samples in metal containers, use low-light detection devices for imaging or photon detection, and use manual or automatic samplers to load aerosol samples. An aerosol post-processing device is used to process the detected aerosol sample, an electric filter turntable is used to achieve sub-band imaging, and an external control system controls each movable component of the present invention for automatic control detection, data processing and detection result reporting.

The supply voltage of the Tesla high-frequency and high-voltage discharger is DC between 1.5-3.0V.

The discharge electrode is installed at an appropriate position inside the metal container and is used for discharging in the metal container.

The metal container is a cylindrical hollow container with an inner diameter of 33 mm, a height of 37 mm, and a hollow volume of 30 ml.

The highly transparent circular glass slide is a circular quartz glass slide with a diameter of 35 mm and a thickness of 1 mm, and is fixedly installed above the metal container.

The electric filter turntable is used to load filters or light traps to assist in low-light spectrum imaging, and the action of the electric filter turntable is controlled by an external control system.

The imaging lens is used for image focusing in low-light imaging and matches the photon imaging device of the photon imaging or detection device.

The aerosol post-processing device is used for safe treatment of aerosol samples after discharge detection, and includes two bottles in series. The first bottle is filled with purified water, and the second bottle is filled with 1% hypochlorite disinfectant. , ensuring that the detected aerosol virus samples can be safely discharged into the air after processing.

The photon imaging or detection device is used to detect weak light signals, and its workflow is controlled by an external control system. The imaging device can be: electron multiplication CCD (EMCCD), image intensification CCD, CMOS, avalanche photodiode APD array, etc. Photon detection can be a low-noise photomultiplier tube (PMT), etc.

The aerosol injector is used for aerosol sampling and can be controlled manually or electrically. The sampling can be combined with or without an aerosol filter.

When using the device of the present invention, it is used together with the ultra-weak biophoton imaging system (UBIS). The ultra-weak biophoton imaging system (UBIS) is a patented technical system with patent number 201310524951.4.

A detection method for real-time monitoring of virus detection devices in aerosols by discharge spectrum imaging, which is characterized by following the following steps: the aerosol low-light radiation spectrum imaging process based on Tesla discharge is completed in 675 seconds, using an electric filter The turntable performs spectral imaging tests of seven different local test windows LTWs, among which LTW-1 and LTW-7 are not loaded with light traps or filters, and the other five LTW-2 to LTW-6 are loaded with light traps of different wavelengths. or filters;

The UBIS system is used to detect weak light radiation. The EMCCD is used as a photon imaging device to extract the aerosol sample and inject it into a metal container. It is completed within 6-8 seconds. An external control system is set up to control the electric filter turntable (5) during imaging. During the process, press the experimental design time switch to complete relevant actions to make the experiment process automatic; after the settings are completed, start aerosol manual injection or automatic injection to complete a test; store a series of image files obtained by EMCCD imaging, and use EMCCD The control program extracts the average gray value GV of each frame of image and stores it in an appropriate data file format for further analysis; the average gray value GV of each frame of image is used to analyze and compare the weak characteristics of aerosol samples in different local test windows. Changes in light radiation; perform data analysis and comparison, use receiver operating characteristic (ROC) curve analysis to evaluate the optimal cutoff value for predicting positive virus detection results, and use area under the curve (AUC) to determine sensitivity and specificity evaluation values , to establish standards for further sample testing.

The present invention can detect and monitor viruses contained in aerosols by real-time imaging of low-light changes caused by Tesla discharge. Although many viruses exist in nature, only a few species are aerosol-transmitted and cause disease in humans and animals. Therefore, this relatively non-specific virus monitoring technology demonstrates the irreplaceable advantages of existing technologies in predicting the emergence of new viruses and early monitoring of known viruses.

Due to the adoption of the above technical solutions, the present invention has the following advantages:

(1) High sensitivity: When Andor iXon ultra-897 EMCCD is used as the photon imaging device, the system sensitivity level reaches 2800 virus particles/microliter (vp/ml).

(2) It has good operability: Because the computer programs the time course controller and then controls the entire imaging test system, the test process is completely automated and remote real-time control can be achieved.

(3) It has a flexible and wide range of applications and can be widely used in various scenarios, such as human life and public activity venues, customs, airports, schools and hospitals, as well as animal breeding and processing venues.

(4) It has good expansion and upgrade potential: In this system, each sub-component is relatively independent and can be flexibly improved, upgraded and expanded according to specific application conditions.

Description of the drawings

Figure 1 is a schematic diagram of the device of the present invention.

Figure 2a shows the characteristic changes of saline water and virus aerosol based on spectral analysis using five different wavelength light traps (488, 514, 533, 561 and 596nm).

Figure 2b is a RGV scatter plot of a representative unfiltered saline (b) aerosol sample.

Figure 2c is a RGV scatter plot of a representative unfiltered virus (c) aerosol sample.

Figure 2d is a plot comparing ARIs or ASC indices between unfiltered and filtered saline aerosols.

Figure 2e is a scatter plot of RGV for a representative filtered virus aerosol sample at medium concentrations.

Figure 2f is a scatter plot of RGV for a representative unfiltered (f) virus aerosol sample at medium concentrations.

Figure 2g is a comparison graph of ARIs or ASC indices between unfiltered and filtered virus aerosols.

Figure 2h is a comparison graph of ARIs or ASC index between unfiltered saline and filtered virus aerosol.

Figure 2i shows that the concentration-dependent change of ASC index was found in the unfiltered virus aerosol sample (r2=0.8668), but not in the filtered sample (r2=0.2874).

Figure 3a is a comparison chart of the ASC index in unfiltered saline aerosols with different concentrations and the ASC index in unfiltered virus aerosols.

Figure 3b is a comparison chart of the ASC index in filtered virus aerosol (b) with different concentrations and the ASC index in unfiltered virus aerosol.

Figures 3c, 3d, 3e, and 3f respectively show the receiver operating characteristic curves (ROC) of the ASC index of unfiltered saline aerosol or filtered virus aerosol as predictors of unfiltered virus aerosol. AUC: area under the curve; the dotted line is the line with no predictive value, that is, AUC=0.5.

Figure 3g is a reference curve showing the ARI-Ratios changes of five test windows in unfiltered saline (S-NF), filtered virus (V-F) and high concentration of unfiltered virus (V-NF-M+H) aerosol samples. .

Detailed ways

In order to better understand the present invention, the specific content, operating procedures and actual performance of the present invention will be further elucidated below in conjunction with the examples. However, the content of the present invention is not limited to the following embodiments. Those skilled in the art can make various improvements, upgrades and expansions to the present invention, and these equivalent forms are also within the scope of the claims listed in this application.

As shown in Figure 1, a discharge spectrum imaging real-time monitoring device for detecting viruses in aerosols includes a Tesla high-frequency and high-voltage discharger 1, a metal container 3, a highly transparent circular glass slide 4, and an electric filter turntable 5 , imaging lens 6, photon imaging or detection device 7, aerosol injector, air pump 10, aerosol post-processing device 11, characterized in that: the discharge electrode 2 of the Tesla high-frequency and high-voltage discharger 1 is inserted into the metal container 3 Inside, the metal container 3 is provided with a highly transparent circular glass slide 4, an air inlet and an air outlet. The air inlet is connected to two aerosol injectors (8, 9) and an air pump 10, and the air outlet is connected to the air pump. The sol post-processing device 11 is connected; an electric filter turntable 5, an imaging lens 6, and a photon imaging or detection device 7 are provided above the highly transparent circular glass slide 4. The photon imaging or detection device 7 passes through the imaging lens 6 and the electric filter. The mirror turntable 5 and the highly transparent circular glass slide 4 realize the imaging or detection of weak light radiation of the aerosol sample in the metal container 3 induced by the discharge electrode. The air pump 10 is used for air or aerosol sample cleaning after detection. The power supply voltage of the Tesla high-frequency and high-voltage discharger 1 is direct current between 1.5-3.0V.

The discharge electrode is installed at an appropriate position inside the metal container and is used for discharging in the metal container.

The metal container 3 is a cylindrical hollow container with an inner diameter of 33 mm, a height of 37 mm, and a hollow volume of 30 ml.

The highly transparent circular glass slide 4 is a circular quartz glass slide with a diameter of 35 mm and a thickness of 1 mm, and is fixedly installed above the metal container.

The electric filter turntable 5 is used to load filters or light traps to assist in low-light spectrum imaging. The movement of the electric filter turntable 5 is controlled by an external control system.

The imaging lens 6 is used for image focusing in low-light imaging and matches the photon imaging device of the photon imaging or detection device 7 .

The aerosol post-processing device 11 is used for safe treatment of aerosol samples after discharge detection, and includes two bottles in series. The first bottle is filled with purified water, and the second bottle is filled with 1% hypochlorite for disinfection. agent to ensure that the detected aerosol virus samples can be safely discharged into the air after processing.

The photon imaging or detection device 7 is used to detect weak light signals, and its workflow is controlled by an external control system. The imaging device can be: electron multiplication CCD (EMCCD), image intensification CCD, CMOS, avalanche photodiode APD array, etc. Photon detection can be a low-noise photomultiplier tube (PMT), etc.

The aerosol sampler (8, 9) is used for aerosol sampling and can be controlled manually or electrically. The sampling can be combined with or without an aerosol filter.

The air pump 10 is used for air cleaning and drying of aerosol samples in metal containers.

When using the device of the present invention, it is used together with the ultra-weak biophoton imaging system UBIS. The ultra-weak biophoton imaging system UBIS is a patented technical system with patent number 201310524951.4.

Utilize the detection device and method of the present invention, use EMCCD (Andorra, iXon ultra-897) as the photon imaging device, and use aerosol samples containing chicken Newtown vaccine virus at different concentrations to detect and evaluate the feasibility of this technology and Sensitivity and specificity.

Example: Detection of chicken Newcastle vaccine virus and assessment of sensitivity and specificity.

1. Testing materials:

The chicken Xincheng vaccine virus stock solution sample used was purchased from a qualified animal vaccine production company through a professional agent. The vaccine virus can be experimented and tested in ordinary biological laboratories.

2. Detection steps

1. Preparation of aerosol virus samples and estimation of virus concentration

The aerosol sample used in the present invention is made of a PVC material to prepare a sealable cubic container with a volume of approximately 3.8L (195mm×140mm×140mm). The medical atomizer outside the container is connected to the atomizer terminal through the outlet tube to atomize the liquid sample in the container to produce aerosol particles smaller than 3 μm. The atomization capacity of the atomizer is approximately 0.3ml/min. If each atomization time is 2 minutes, a 2ml liquid sample can be repeatedly atomized three times. Therefore, by referring to the total number of viruses in the liquid virus sample added to the nebulizer terminal each time, the virus concentration in the aerosol generated after 2 minutes of nebulization can be estimated:

The virus concentration in the aerosol (virus particles/microliter) ≈ the total number of viruses in the sample × 158 × 10 ^-6 (0.6/3800).

The three aerosol virus concentrations tested in this example were 280, 2800 and 28000 virus particles/microliter (vp/ml).

2. Testing process

The aerosol low-light radiation spectrum imaging process based on Tesla discharge was completed in 675 seconds (11.25 minutes) (Figure 2a). After injection of the aerosol sample with or without a PES filter, spectral imaging tests of seven different local test windows (LTW) were performed using a motorized light trap or filter wheel, among which LTW-1 and LTW-7 is not loaded with light traps or filters, while the other five LTWs (LTW-2 to LTW-6) are loaded with light traps or filters of different wavelengths. The specific testing process is as follows:

1) Set up a low light detection system.

Weak light radiation was detected using the UBIS system. EMCCD is used as a photon imaging device. The key parameters of EMCCD imaging are: (1) The cooling temperature of EMCCD reaches -80°C; (2) The exposure time of each frame is 500ms; (3) Under the normal model, the gain of EMCCD is 20; (4) The image readout mode is 1×1.

2) Sampling, filtration and injection

To ensure complete replacement of trapped air in the injection tube (approximately 20ml, 160mm length, 4mm inner diameter) and detection container (approximately 30ml), 100ml aerosol sample was used for each test. According to the test plan, immediately after the atomization is completed, use an atomization syringe (250ml) to manually extract a 200ml aerosol sample from an interface in the middle of the atomization container, and inject the 100ml sample into the detection system through the sample injection port according to the test process. And complete within 6-8 seconds. Another 100ml sample is waiting for the next test. The method is the same, but it is filtered with a PES membrane filter (0.02μm) during the bolus injection process to compare and test the difference between the same aerosol sample under filtered and non-filtered conditions.

3)Sample test

Start formal imaging: Set the schedule controller on the computer. Each movable component of the external control system, including the electric light trap or filter wheel, switches on and off according to the experimental design during the imaging process to complete relevant actions and make the experimental process automatic. After the settings are completed, start aerosol injection and automatically complete a test as described above.

3. Data storage and retrieval

Store a series of image files obtained by EMCCD imaging, use EMCCD control software to extract the average gray value (GV) of each frame of image, and store it in an appropriate data file format for further analysis.

4. Data analysis and algorithm of aerosol spectral change index (ASC-index)

The average gray value (GV) of each frame image is used to analyze and compare the low-light radiation changes of aerosol samples in different local test windows (LTWs).

The calculation definition of relative GV (RGV) of the local test window is as follows:

RGV＝Y-X

Y is the GV of the discharge test, and X is the GV of the non-discharge background in the frame image.

The average radiant intensity (ARI) of an aerosol sample is defined by the relative gray value (RGVs).

The average radiation intensity ratio (ARI-Ratio) is calculated as follows:

ARI-Ratio＝Y/X

While Y is the average RGVs of the starting LTW of the seven test LTWs, X is the average RGV (ARI-ratio _AE ) of one of the five LTWs with a trapper.

The algorithm of aerosol spectral change index (ASC-index) is defined as follows:

ASC-index＝ARI-ratio _A +ARI-ratio _B +ARI-ratio _C +ARI-ratio _D +ARI-ratio _E

1. Establishment of testing standards

Data analysis and comparisons were performed using a combination of commercial software programs. Receiver operating characteristic (ROC) curve analysis was used to evaluate the optimal cutoff value for predicting a positive viral test result. The area under the curve (AUC) was used to determine sensitivity and specificity evaluation values to establish standards for further sample testing.

3. Test results

Figure 2b and Figure 2c are scatter plots of the relative gray value of the imaging results of two representative saline aerosol samples detected under PES filtered (S-F) and unfiltered (S-NF) conditions. Statistical analysis of the ARI- There is no significant difference between ratios and ASC-index (Fig. 2d), which indicates that aerosol particle size cannot change the spectral characteristics.

Figure 2e and Figure 2f are the relative gray value scatter plots of the imaging results of two representative viral aerosol samples detected under PES filtered (V-F) and unfiltered (V-NF) conditions. Statistical analysis of the ARI-ratios of five LTWs and ASC-index were significantly different (Fig. 2g), in which the ARI ratio and ASC-index in filtered virus aerosols were significantly lower than those in unfiltered aerosols, but were different from those in unfiltered saline aerosols. The ARI ratio in the sol increased significantly compared to that in the sol (Fig. 2h).

The ASC-index in unfiltered virus aerosol showed a concentration-dependent change (r2=0.8668), but there was no change after filtration ( ^r2 =0.2874) (Fig. 2i).

The sensitivity and specificity of unfiltered virus aerosol (V-NF) were evaluated using the ASC-index of unfiltered saline (S-NF) or filtered virus (V-F) aerosol as a reference (Fig. 3a, Fig. 3b). Regardless of concentration differences, the ROC curve for ASC-index identifying viral aerosols showed an area under the curve (AUC) of 0.79 (95% confidence interval, 0.69 to 0.89, p<0.0001) (S-NF vs. V-NF) or 0.84 (95% confidence interval, 0.75 to 0.93, p<0.0001) (V-F vs. V-NF). The two cutoff values of ASC--index (>3.166 and >3.167) showed the best sensitivity and specificity, respectively 0.71 (95% confidence interval, 0.54 to 0.85) and 0.72 (95% confidence interval, 0.72). 0.56 to 0.85) (ratio of S-NF to V-NF) (Fig. 3c), and 0.70 (95% confidence interval, 0.53 to 0.84) and 0.76 (95% confidence interval, 0.59 to 0.88) (ratio of V-F to V -NF ratio) (Fig. 3d). ROC curves for ASC-index identifying high concentration combinations of viruses in aerosols showed an area under the curve (AUC) of 0.80 (95% confidence interval, 0.69 to 0.90, p<0.0001) (S-NF vs. V-NF) or 0.85 (95 % confidence interval, 0.75 to 0.94, p<0.0001) (V-F vs. V-NF). The two cutoff values of ASC-index (>3.167 and >3.168) showed the best sensitivity and specificity, respectively 0.71 (95% confidence interval, 0.52 to 0.86) and 0.72 (95% confidence interval, 0.56 to 0.86). 0.85) (ratio of S-NF to V-NF) (Fig. 3e), and 0.70 (95% confidence interval, 0.51 to 0.85) and 0.76 (95% confidence interval, 0.59 to 0.88) (ratio of V-F to V-NF ratio) (Figure 3f).

These results show that the sensitivity and specificity of this new detection method can reach 70% and 76% when the aerosol virus concentration is higher than 2800 particles per microliter (vp/ml). Therefore, a test reference standard (RS) can be established by using the ASC-index with a cutoff value (>3.168) and two reference curves (positive and negative) that show the five Changes in the ARI ratio of species LTW (Fig. 3g). If the ASC index is ≥3.168 and the curve of the test sample overlaps or exceeds the positive RS curve of unfiltered virus aerosol, it means that the test sample is positive.

The above is only used to illustrate specific implementation examples of the present invention, and is not intended to limit the scope of the present invention. It refers to everything accomplished by those skilled in the art without departing from the spirit and principles indicated by the present invention. Equivalent improvements, upgrades, expansions, etc. should still be covered by the scope of the claims of the present invention.

Claims (9)

1. A discharge spectrum imaging real-time monitoring device for detecting viruses in aerosols, including a Tesla high-frequency and high-voltage discharger (1), a metal container (3), a highly transparent circular glass slide (4), and an electric filter turntable ( 5), imaging lens (6), photon imaging or detection device (7), aerosol injector, aerosol post-processing device (11), characterized by: the discharge of Tesla high-frequency and high-voltage discharger (1) The electrode (2) is inserted into the metal container (3). The metal container (3) is provided with a highly transparent circular glass slide (4), an air inlet and an air outlet. The air inlet is connected to the aerosol sampler, and the air outlet is connected to the aerosol sampler. The air port is connected to the aerosol post-processing device (11); an electric filter turntable (5), an imaging lens (6), a photon imaging or detection device (7), and a photon imaging device (7) are provided above the highly transparent circular glass slide (4). The imaging or detection device (7) realizes weak light radiation of the aerosol sample in the metal container (3) induced by the discharge electrode through the imaging lens (6), the electric filter turntable (5) and the highly transparent circular glass plate (4) imaging or detection.
2. The device for real-time monitoring of viruses in aerosols by discharge spectrum imaging according to claim 1, characterized by further comprising: an air pump (10), and the air pump (10) is connected to the air inlet of the metal container (3).
3. A discharge spectrum imaging real-time monitoring virus detection device in aerosols according to claim 1 or 2, characterized in that: the power supply voltage of the Tesla high-frequency and high-voltage discharger (1) is between 1.5-3.0 V of direct current.
4. A discharge spectrum imaging real-time monitoring virus detection device in aerosols according to claim 1 or 2, characterized in that: the metal container (3) is a cylindrical hollow container with an inner diameter of 33 mm in diameter and a height of 37 mm mm, hollow volume 30 ml.
5. A discharge spectrum imaging real-time monitoring device for detecting viruses in aerosols according to claim 1 or 2, characterized in that: the highly transparent circular glass slide (4) is a circular quartz glass slide with a diameter of 35 mm. , 1 mm thick, fixedly installed above the metal container.
6. A discharge spectrum imaging real-time monitoring virus detection device in aerosols according to claim 1 or 2, characterized in that: the electric filter turntable (5) is used to load filters or light trapping films to assist weak Optical spectrum imaging, the movement of the electric filter turntable (5) is controlled by an external control system.
7. A discharge spectrum imaging real-time monitoring virus detection device in aerosol according to claim 1 or 2, characterized in that: the imaging lens (6) is used for image focusing of low-light imaging, and is combined with the photon imaging or detection device ( 7) Matching of photonic imaging devices.
8. A discharge spectrum imaging real-time monitoring virus detection device in aerosol according to claim 1 or 2, characterized in that: the aerosol post-processing device (11) is used for safe processing of aerosol samples after discharge detection, including Two bottles in series, the first containing purified water and the second containing 1% hypochlorite disinfectant, ensure that the detected aerosol virus samples can be safely discharged into the air after processing.
9. A detection method for real-time monitoring of virus detection devices in aerosols using discharge spectrum imaging according to claim 1 or 2, which is characterized in that the following steps are performed: the aerosol low-light radiation spectrum imaging process based on Tesla discharge is performed at 675 Completed within seconds, use the electric filter turntable (5) to conduct spectral imaging tests of seven different local test windows LTWs, of which LTW-1 and LTW-7 are not loaded with light traps or filters, and the other five LTW- 2 to LTW-6 are loaded with light traps or filters of different wavelengths;

   The UBIS system is used to detect weak light radiation. The EMCCD is used as a photon imaging device to extract the aerosol sample and inject it into a metal container. It is completed within 6-8 seconds. An external control system is set up to control the electric filter turntable (5) during imaging. During the process, the timing switch is pressed according to the experimental design to complete the relevant actions to make the experiment process automatic; after the setting is completed, start aerosol manual injection or automatic injection to complete one test each; store a series of image files obtained by EMCCD imaging, and use The EMCCD control program extracts the average gray value GV of each frame image and stores it in an appropriate data file format for further analysis; the average gray value GV of each frame image is used to analyze and compare aerosol samples in different local test windows LTWs Changes in low-light radiation; perform data analysis and comparison, use receiver operating characteristic (ROC) curve analysis to evaluate the optimal cutoff value for predicting positive virus detection results, and use area under the curve (AUC) to evaluate sensitivity and specificity values determination to establish standards for further sample testing.

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