WO2023193485A1

WO2023193485A1 - Composite structure micro-bottle lens, and super-resolution imaging system based on micro-bottle lens

Info

Publication number: WO2023193485A1
Application number: PCT/CN2022/142548
Authority: WO
Inventors: 顾国强; 杨慧
Original assignee: 深圳先进技术研究院
Priority date: 2022-04-06
Filing date: 2022-12-27
Publication date: 2023-10-12

Abstract

The present application provides a preparation method for a composite structure micro-bottle lens, and a composite structure micro-bottle lens. A trace amount of a polymer micro-liquid is adhered to a semi-conical micro-optical fiber; one or more sections of the adhered trace amount of polymer liquid is then controllably transferred to another semi-conical micro-optical fiber, so as to form a covering film with a bottle-shaped contour, and the covering film constitutes a composite structure micro-bottle lens together with the micro-optical fiber; and finally, the liquid covering film part of the composite structure micro-bottle lens is converted into a solid state in a photocuring or thermal curing manner, so as to form a stable and easily operated composite structure micro-bottle lens.

Description

Composite structure microbottle lens and super-resolution imaging system based on microbottle lens

This application requires a Chinese patent application submitted to the China Patent Office on April 6, 2022, with the application number CN202210356452.8 and the invention name "A preparation method of a composite structure microbottle lens and a composite structure microbottle lens" and in 2022 The Chinese patent application priority was submitted to the Chinese Office on April 6 with the application number CN202210356445.8 and the invention title "A super-resolution imaging system and imaging method based on microbottle lenses", the entire content of which is incorporated into this application by reference. middle.

Technical field

The present application relates to the technical field of optical elements, and in particular to a composite structure microbottle lens and a super-resolution imaging system based on the microbottle lens.

Background technique

Composite structure microbottle lenses, whose early names include spindle section microfibers, microbottle resonant cavities, etc., belong to the field of fibers and optical microcavities. Among them, spindle section microfibers are used in fields such as water collection, oil-water separation, microfluidics, and self-cleaning. Widely used, microbottle resonators have important applications in delay lines, microlasers, nonlinear optics, optomechanics, sensing and other fields. The most similar implementation scheme to the present invention, the earliest preparation method was proposed by me in 2013 and 2014 (Patent No.: ZL201310268542.2; Applied Optics,2014,53:7819‑7824). Among them, the invention patent "ZL201310268542.2" is a microbottle resonant cavity that relies on bare optical fibers or microfibers to prepare optical adhesive patches. The material used is NOA 61 optical adhesive patches produced by the American Norland Company. Its purpose is to make echo walls. Membrane optical micro-resonant cavity device, the preparation method is to pass the NOA through a semi-tapered optical fiber 61 Optical adhesive droplets are dropped into the taper waist of standard single-mode optical fiber or full-taper optical fiber formed by thermal drawing method. The liquid surface tension and viscous force effects form a single bottle-shaped micro-resonant cavity on the bare optical fiber or micro-fiber. ;After publishing the article "Applied Optics, 2014, 53:7819-7824", NOA 61 optical adhesive produced by Norland Company is also used, relying on the micro-fiber formed by the molten tapering, using the wetting and liquid surface tension effects of the adhesive on the micro-fiber. Optical micro-resonant cavities that are self-assembled on micro-fibers to form multiple continuous bottle-shaped structures are also used in whispering gallery optical micro-cavities.

The main shortcomings of the existing technology are: 1) Because the devices are all used in optical microcavity devices, the optical adhesives have strict limitations, usually requiring a wide light transmission bandwidth, a low light attenuation rate, and It has the characteristics of low shrinkage under light irradiation and high hardness after curing; 2) The method used is to use a semi-tapered optical fiber to drop micro droplets into a full-tapered optical fiber to form a microbottle resonant cavity. Obviously the dripping method It is necessary to use the gravity of the droplets to overcome the adhesion force of the microfiber, so the microdroplets cannot be too small, otherwise they cannot be separated from the semi-tapered microfiber; 3) Because it relies on the full-tapered fiber to allow the microfiber to adhere to the The optical adhesive liquid naturally breaks and shrinks to form a micro-bottle resonant cavity, which cannot be controlled externally. Therefore, the number and size of the formed micro-bottle resonant cavities cannot be controlled.

technical problem

In view of this, it is necessary to provide a preparation method for forming a stable and easy-to-operate composite structure microbottle lens and a composite structure microbottle lens to address the shortcomings in the prior art.

Technical solutions

In a first aspect, embodiments of the present application provide a composite structure microbottle lens, which is characterized in that the composite structure microbottle lens includes a microfiber and a covering film covering the microfiber.

In a second aspect, embodiments of the present application also provide a super-resolution imaging system based on a microbottle lens, which is characterized in that it includes: a microbottle lens module, a position control module and an optical microscope; the microbottle lens module includes the above composite Structural microbottle lenses;

The position control module can control the rotation of the microbottle lens. The sub-diffraction limit scale sample to be imaged is installed on the stage of the optical microscope. The microbottle lens is arranged on the sub-diffraction limit scale sample to be imaged. Above, the microbottle lens and the optical microscope can be used to perform super-resolution imaging of the sub-diffraction limit scale sample to be imaged.

In a third aspect, embodiments of the present application also provide a method for preparing a composite structure microbottle lens, which includes the following steps:

Two sections of semi-tapered microfibers are provided, wherein the thinnest part of one end of the semi-tapered microfiber and the area with equal diameters is the tapered waist region, and the diameter of the microfiber gradually becomes smaller between the bare optical fiber and the tapered waist region. The area is a tapered transition zone;

Transfer the polymer microliquid to one of the tapered transition zones;

Slide the polymer microfluid along the tapered transition zone to the tapered waist zone, and partially adhere to the semi-tapered microfiber;

The polymer liquid attached to the semi-tapered microfiber forms several tiny droplets;

Transfer the tiny droplets to the tapered waist of another section of the semi-tapered microfiber;

The tiny droplets transferred to the tapered waist of another section of the semi-tapered microfiber form a liquid covering film, and form a microbottle lens with a composite structure with the wrapped microfiber;

The liquid covering film is solidified to form a solid composite structure microbottle lens.

beneficial effects

This application adopts the above technical solution, and its beneficial effects are as follows:

The preparation method of a composite structure microbottle lens and the composite structure microbottle lens provided by this application first adhere a trace amount of polymer microliquid to a semi-tapered microfiber, and then controllably attach one or more segments of the adhered trace amount of polymer liquid. is transferred to another semi-tapered microfiber to form a covering film with a bottle-shaped outline, and together with the microfiber to form a composite microbottle lens, the composite microbottle lens is finally cured by light or heat curing. The liquid covering film is partially converted into a solid state, forming a stable and easy-to-operate composite structure microbottle lens.

The composite structure microbottle lens provided by this application can be used as a microlens and is used in fields such as light focusing, optical imaging, and signal enhancement.

Description of the drawings

Figure 1 is a schematic structural diagram of the preparation method of a composite structure microbottle lens provided by this application.

Figure 2 is a front view of the composite structure microbottle lens provided by this application.

Figure 3 is a schematic structural diagram of the composite structure microbottle lens provided by this application in the left direction.

Figure 4 is a cross-sectional view of the composite structure microbottle lens provided by this application.

Figure 5 is a flow chart for the preparation of the composite structure microbottle lens provided in Example 1 of the present application.

Figure 6 is a composite structure microbottle lens obtained by curing PDMS using a thermal curing method provided in Example 1 of the present application.

Figure 7 is a microscopic image of a composite structure microbottle lens obtained by curing ultraviolet glue using the light curing method provided in Example 1 of the present application.

Figure 8 is a schematic structural diagram of the microbottle lens-based super-resolution imaging system provided by this application.

Figure 9 is a schematic structural diagram of the microbottle lens module provided by this application.

Figure 10 is a front view and a left view of the composite structure microbottle lens provided by the present application.

Figure 11 is a schematic structural diagram of the position control module provided by this application.

Figure 12 is a front view and a top view of a sub-diffraction limit scale sample to be imaged provided by this application.

Figure 13 is a flow chart of the steps of the microbottle lens-based imaging method provided by this application.

Figures 14(a) and (b) respectively show the simulation results of the focused light field formed by plane wave irradiation of the SiO2 micro-cylindrical lens and the SiO2 micro-bottle lens provided in this embodiment.

Figure 15(a) shows a composite structure microbottle lens prepared by relying on microfibers using self-assembly and photocuring methods in this embodiment.

Figure 15(b) is a microscopic image of the detected sub-diffraction limit scale sample provided in this embodiment.

Figure 15(c) is a digital format image obtained by introducing a composite structure microbottle lens for imaging provided in this embodiment.

Figure 15(d) is the light intensity distribution diagram of the horizontal line marked in Figure 15(c).

Detailed ways

The embodiments of the present application are described in detail below. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements with the same or similar functions. The embodiments described below with reference to the drawings are exemplary and are intended to explain the present application, but should not be construed as limiting the present application.

In the description of this application, it should be understood that the orientation or positional relationship indicated by the terms "upper", "lower", "level", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings. , is only for the convenience of describing the present application and simplifying the description, but does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation of the present application.

In addition, the terms “first” and “second” are used for descriptive purposes only and cannot be understood as indicating or implying relative importance or implicitly indicating the quantity of indicated technical features. Therefore, features defined as "first" and "second" may explicitly or implicitly include one or more of these features. In the description of this application, "plurality" means two or more than two, unless otherwise explicitly and specifically limited.

In order to make the purpose, technical solutions and advantages of the present application more clear, the present application will be further described in detail below with reference to the drawings and embodiments.

Please refer to Figure 1, which is a flow chart of a method for preparing a composite structure microbottle lens provided in this embodiment, which includes the following steps:

Step S110: Provide two sections of semi-tapered microfiber.

In this embodiment, a semi-tapered microfiber is prepared based on the thermal drawing method, which specifically includes the following steps:

Step S111: Peel off the coating layer of the middle part of the optical fiber to obtain a bare optical fiber including a cladding layer and a core layer.

Specifically, a section of cylindrical optical fiber with a length of more than 100 mm is selected. In the middle of the cylindrical optical fiber, use a wire stripper to strip off the coating layer with a length of about 30 mm to obtain a bare optical fiber including a cladding and a core layer.

It can be understood that the optical fiber provided in this embodiment may include polymer optical fiber, special material optical fiber; or may also use natural fibers such as spider silk/silk, silk artificial fibers, etc.

Specifically, the thinnest part of one end of the semi-tapered microfiber and the area with the same diameter is the tapered waist region, and the area where the diameter of the microfiber gradually decreases from the bare optical fiber to the tapered waist region is the tapered transition zone.

Step S112: Stretch the bare fiber to obtain a tapered microfiber, in which the thinnest part in the middle and the area with the same diameter is the tapered waist area, and the area between the bare fiber and the tapered waist where the diameter of the microfiber gradually becomes smaller is Tapered transition zone.

In this embodiment, the step of stretching the bare fiber to obtain a tapered microfiber specifically includes: using a stepper motor to stretch the bare fiber under heating conditions to obtain a tapered microfiber.

It can be understood that the taper and length of the tapered microfiber can be controlled by factors such as the stepper motor stretching speed, the stretching distance, and the width of the heating flame.

Step S113: Pull off the tapered microfiber to form two sections of semi-tapered microfiber.

It can be understood that the tapered microfiber is stretched and broken, and the full-tapered microfiber is pulled off from the middle to form a semi-tapered microfiber.

Step S120: Transfer the polymer microliquid to the tapered transition zone of one section of the semi-tapered microfiber.

In this embodiment, the step of transferring the polymer microfluid to the tapered transition zone of one section of the semi-tapered microfiber specifically includes the following steps: using a syringe to transfer the polymer microfluid to the semi-tapered microfiber. In the tapered transition zone of the microfiber, the polymer microliquid includes UV glue or PDMS.

Step S130: Slide the polymer microfluid along the tapered transition zone to the tapered waist zone, and partially adhere to the semi-tapered microfiber.

In this embodiment, the step of sliding the polymer microfluid along the tapered transition zone to the tapered waist zone and partially attaching it to the semi-tapered microfiber specifically includes the following steps:

The semi-tapered microfiber is erected in a direction from the tapered transition area to the tapered waist area, so that the polymer droplets follow the tapered transition of the semi-tapered microfiber under the action of gravity. The region slides down to the tapered waist region and is partially attached to the semi-tapered microfiber.

Step S140: The polymer liquid attached to the semi-tapered microfiber forms several tiny droplets.

It can be understood that the adhered polymer liquid forms tiny oblong droplets under the action of surface tension and viscosity.

Step S150: Transfer the tiny droplets to the tapered waist of another section of the semi-tapered microfiber.

In this embodiment, the step of transferring the tiny droplets to the tapered waist of another section of the semi-tapered microfiber specifically includes the following steps: using precision mechanical control to transfer the tiny droplets to the tapered waist of another semi-tapered microfiber.

Furthermore, a small amount of tiny droplets is transferred to the tapered waist of another semi-tapered optical fiber through a high-precision three-dimensional adjustment stand, achieving controllable preparation in terms of quantity and size.

Step S160: The tiny droplets transferred to the tapered waist of another section of the semi-tapered microfiber form a liquid covering film, and form a microbottle lens with a composite structure with the wrapped microfiber.

It can be understood that the tiny droplets transferred to the waist of the cone form a liquid covering film with a microbottle structure on the microfiber under the combined action of liquid surface tension, viscosity force and Prato-Rayleigh instability.

In this embodiment, the liquid covering film has a microbottle structure. Transfer one or more of the liquid covering films at the waist of the microfiber taper.

Step S170: Solidify the liquid covering film to form a solid composite structure microbottle lens.

In this embodiment, the step of solidifying the liquid covering film to form a solid composite structure microbottle lens specifically includes the following steps: solidifying the liquid covering film through a light or thermal curing operation to form a solid composite structure microbottle lens. Bottle lens.

The method for preparing a composite structure microbottle lens provided by this application first adheres a trace amount of polymer microliquid to a semi-tapered microfiber, and then controllably transfers one or more segments of the adhered trace polymeric liquid to another fiber. On the semi-tapered microfiber, a bottle-shaped covering film is formed, and together with the microfiber, it forms a composite microbottle lens. Finally, the liquid covering film part of the composite microbottle lens is converted into a composite microbottle lens using light curing or thermal curing. Solid state, forming a stable and easy-to-operate composite structure microbottle lens.

This application also provides a composite structure microbottle lens prepared by the preparation method of a composite structure microbottle lens. The composite structure microbottle lens includes a microfiber and a covering film covering the microfiber. The covering film The film is obtained by curing the liquid covering film.

Please refer to Figures 2 to 4, wherein Figure 2 is a front view of a composite structure microbottle lens, in which mark L is the length of the microbottle lens in the transverse direction. Figure 3 is a schematic structural diagram of a composite structure microbottle lens in the left-view direction, in which the mark Di is the diameter of the cylindrical microfiber, and Do is the diameter of the middle of the liquid covering film of the microbottle lens. Figure 4 is a cross-sectional view of a composite structure microbottle lens. L, Di and Do are respectively the length of the microbottle lens in the transverse direction, the diameter of the cylindrical microfiber, and the diameter at the middle position of the liquid covering film.

It can be understood that the composite structure microbottle lens prepared in this application can form bottle-shaped profiles with different curvatures or degrees of bending according to the viscosity and hardness of the curable material itself and the interface effect with the microfiber material. Microbottle lens; the above-mentioned composite structure microbottle lens is not limited to the double-layer structure shown in this embodiment. The semi-tapered microfiber can be made of a single material, a variety of materials, or multiple layers of materials. The curable polymer composed of a core-shell or concentric structure and a bottle-shaped outline can also be a single-layer or multi-layer structure. The multi-layer structure can be made of different types of curing materials, such as light-curing or thermal-curing materials. The layer structure can also be a multi-layer structure composed of photo-curing and thermo-curing materials.

The composite structure microbottle lens provided in the embodiment of the present application can be used as a microlens and applied in fields such as light focusing, optical imaging, and signal enhancement.

The above technical solution of the present application will be described in detail below with reference to specific embodiments.

Example 1

Please refer to Figure 5, which is a flow chart for the preparation of the composite structure microbottle lens provided in this embodiment 1, in which the cylindrical optical fiber 1, the optical fiber coating layer 2, the bare optical fiber 3, the full-tapered microfiber 4, the semi-tapered microfiber Optical fiber 5, syringe 6, polymer microfluid 7, polymer microfluid 8, liquid covering film 9, tiny droplets 10, high-precision three-dimensional adjustment frame 11, glass slide 12, tape 13, microfiber supporting structure 14, quilt Transferred curable polymer tiny droplets 15, liquid covering film 16, curing instrument 17, composite structure microbottle lens 18. The detailed steps have been explained above and will not be repeated here.

Please refer to Figure 6 , which shows a microbottle lens obtained by curing PDMS using a thermal curing method in this embodiment. The covering film is PDMS polymer, and the supporting optical fiber is silica. The weight ratio of PDMS to SYLGARD 184 curing agent is 10:1, the oven temperature is 80°C, and the heating and curing time is about 10 minutes. The parameters of the microbottle lens are: L=89.2μm, Di=19.9μm, Do=38.4μm.

Please refer to Figure 7, which is a microscopic image of a composite structure microbottle lens obtained by curing UV glue using the light curing method in Example 1. The UV glue used is NOA61 glue patch produced by Norland Company in the United States. The UV lamp used for curing is a UV lamp with a wavelength of 365nm and a power of 60W. The curing time is about 5‑8 minutes. The parameters of the microbottle lens composed of UV glue covering film and microfiber are: L=48.7μm, Di=13.4μm, Do=20μm. By controlling the size of the microfibers used and the amount of curable polymer transferred, microbottle lenses with composite structures can be controllably prepared.

Please refer to FIG. 8 , which is a schematic structural diagram of a super-resolution imaging system based on a microbottle lens provided in this embodiment. In this embodiment, the microbottle lens-based super-resolution imaging system includes: a microbottle lens module 110 , a position control module 120 and an optical microscope 130 . The specific structure and implementation of each module are described in detail below.

Please refer to Figure 9, which is a schematic structural diagram of the microbottle lens module provided by this application. The microbottle lens module 110 includes a microbottle lens 1. The microbottle lens 1 includes a microfiber and a device that covers the microfiber. polymer liquid membrane.

Specifically, a composite structure transparent medium microbottle lens is produced using microfibers using self-assembly and light curing methods, in which the cylindrical microfiber constitutes the middle part of the microbottle lens, and the polymer liquid film covering the middle part constitutes the microbottle lens. Peripheral part. The above-mentioned micro optical fiber and polymer liquid film can be prepared by heating, stretching or softening and compressing methods.

It can be understood that the size of the polymer liquid film is determined by the size of the microfiber, the amount of transferred polymer droplets, the viscosity/hardness of the polymer itself, and the interfacial interaction between the microfiber and the polymer liquid.

Please refer to Figure 10, which is a front view and a left view of the microbottle lens of the composite structure provided by the present application. Where L is the length of the microbottle lens along the axial direction of the microfiber, Di is the diameter of the microfiber, and Do is the maximum diameter of the bottle-shaped outline of the polymer liquid film along the axial direction perpendicular to the microfiber.

In some embodiments, as shown in Figure 8, the microbottle lens module 110 also includes an optical fiber handle 2, and both ends of the optical fiber handle 2 are connected to the microbottle lens 1 and the position control module 120 respectively. .

It can be understood that the microbottle lens module 110 can also be fixed to the position control module 120 in other ways, for example: the microbottle lens 1 is fixed on the tip of a micropipette or embedded in a polymer film or Fixed on the microrobot probe or AFM tip or silicon holder or metal frame or plastic adapter or microscope objective.

Please refer to FIG. 11 , which is a schematic structural diagram of the position control module 120 provided in this application. In this embodiment, the position control module 120 includes a right-angle bracket 4 , an optical adjustment bracket 6 , a post 7 and a 3D nano-translation stage 8 . One end of the right-angle bracket 4 is fixedly connected to the fiber optic handle 2, and the other end is fixedly connected to the optical adjustment frame 6. The optical adjustment frame 6 is fixedly installed on the post 7, and the bottom end of the post 7 It is fixed on the 3D nano translation stage 8, and the position of the microbottle lens 1 in the three coordinate axes directions of x, y and z can be controlled by the 3D nano translation stage 8.

In some embodiments, the right-angle bracket 4 is an "L-shaped" aluminum bracket. It can be understood that the right-angle bracket 4 used can also be replaced by a designed inclined bracket.

Further, as shown in FIG. 8 , the optical fiber handle 2 is fixed on the long side of the right-angle bracket 4 using adhesive tape 3 . The tape 3 is a conventional transparent tape or a Kapton residue-free adhesive tape.

Further, the short side of the right-angle bracket 4 is locked on the optical adjustment frame 6 through screws 5. The screws 5 are M4 stainless steel cap screws.

Furthermore, the optical adjustment mount 6 is a compact optical adjustment mount with M4 mounting screw holes and an angle range of ±4°.

In some embodiments, the 3D nano-translation stage 8 is a three-axis flexible displacement stage with a maximum stroke of 4 mm for each axis, a coarse adjustment stroke of 4 mm, a fine adjustment stroke of 300 μm, and a fine adjustment resolution of 100 nm.

Please refer to Figure 12, which is a front view and a top view of a sub-diffraction limit scale sample to be imaged in this application. Wherein, the microbottle lens is disposed above the sub-diffraction limit scale sample to be imaged.

In this embodiment, the sub-diffraction limit scale sample to be imaged is usually a periodic nanostructure sample, such as a grating structure or a nanodisk array structure, a periodic grating with a line width lw=150nm and a groove width gw=150nm. Striped structure.

In this embodiment, as shown in FIG. 8 , the optical microscope 130 is a conventional optical microscope, including a stage 10 , a longitudinal moving handwheel 11 , a transverse moving handwheel 12 , a coarse adjustment knob 13 , a fine adjustment knob 14 , and a mirror. Base 15, mirror arm 16, observation head 17, converter 18, microscope objective 19, eyepiece 20 and lens barrel 21. Its detailed working method will not be described here.

In some embodiments, a computer module 140 is also included. The computer module 140 includes a charge-coupled element CCD 22 connected to the lens barrel 21 of the optical microscope 130 and a computer 23. The charge-coupled element CCD 22 is used to obtain The image of the optical microscope is converted into a digital format image, and the computer 23 is used to receive and process the digital format image.

In the super-resolution imaging system based on microbottle lenses provided by this application, the position control module 120 can control the rotation of the microbottle lens 1, and the sub-diffraction limit scale sample to be imaged is installed on the stage 10 of the optical microscope 130. , the microbottle lens 1 is arranged above the sub-diffraction limit scale sample to be imaged, and the microbottle lens 1 and the optical microscope 130 can be used to perform super-resolution on the sub-diffraction limit scale sample to be imaged. Imaging. This application uses dielectric microbottle lenses, combined with conventional optical microscopes, to construct a super-resolution imaging method and system assisted by long focal depth and long working distance particle lenses, which can achieve non-contact, non-destructive and non-invasive imaging of samples below the diffraction limit. Imaging of contamination.

The microbottle lens provided in this application is not limited to super-resolution imaging, but can also be used in fields such as microfibers, optical microcavities, optical tweezers, and micro-control.

Please refer to FIG. 13 , which is a flow chart of the imaging method of a microbottle lens-based super-resolution imaging system provided in this embodiment. In this embodiment, the imaging method includes the following steps:

Step S110: Provide the microbottle lens, the microbottle lens module includes a microbottle lens, the microbottle lens includes a microfiber and a polymer liquid film covering the microfiber;

Step S120: Install the microbottle lens, which is disposed above the sub-diffraction limit scale sample to be imaged.

In this embodiment, the following steps are specifically included:

S1. Fix the microbottle lens 1 on the right-angle bracket 4 with the tape 3 through the fiber optic handle 2. The right-angle bracket 4 is locked on the optical adjustment frame 6 through the screw 5. The diameter range of the microfiber is about 2-50 μm, and the tape is conventional. Transparent tape or Kapton residue-free adhesive tape. The right-angle bracket is an "L-shaped" aluminum bracket with a thickness of 2mm. The long and short sides are 160mm and 14mm respectively. The screws are M4 stainless steel cap screws. The optical adjustment mount is with Compact optical mount with M4 mounting screw holes and angle range of ±4°;

S2. The optical adjustment frame 6 is connected and fixed through one end of the post 7, and the other end of the post 7 is fixed on the 3D nano translation stage 8. The 3D nano translation stage 8 is a three-axis flexible displacement stage, with a maximum stroke of 4mm for each axis. The coarse adjustment stroke is 4mm, the fine adjustment stroke is 300μm, and the fine adjustment resolution is 100nm.

S3. Use the 3D nano translation stage 8 to control the position of the microbottle lens 1 in the three coordinate axes directions of x, y, and z, and make the microbottle lens 1 in the center of the sub-diffraction limit scale sample to be imaged. above.

Step S130: Adjust the position of the sub-diffraction limit scale sample to be imaged;

In this embodiment, the following steps are specifically included:

S4. Place the sub-diffraction limit scale sample 9 to be imaged on the stage 10, and control the movement of the stage 10 in the x and y directions by moving the handwheel 11 longitudinally and the handwheel 12 transversely, that is, controlling The position of sample 9 on the stage in the horizontal direction. The coarse adjustment knob 13 and the fine adjustment knob 14 control the up and down movement of the sample 9 in the z direction. The adjustment range of the coarse adjustment knob 13 is about 24mm, and each rotation is about 2mm. Each rotation of the adjustment knob 14 is about 200 μm. The stage 10, the coarse adjustment knob 13 and the fine adjustment knob 14 are installed on the mirror base 15, and are connected to the observation head 17 and the converter 18 through the mirror arm 16;

S5. Install microscope objectives 19 with different magnifications and numerical apertures such as 5×, 10×, 40×, 63× and 100× on the converter 18 .

Step S140: Use the microbottle lens and the optical microscope to perform super-resolution imaging on the sub-diffraction limit scale sample to be imaged.

It can be understood that the formed image can be directly observed through the eyepiece 20 through the observation head 17, or received by the charge-coupled original CCD 22 installed on the upper end of the lens barrel 21, and transmitted to the computer 23 for data processing to obtain a digital format image.

This application provides an imaging method for a super-resolution imaging system based on a microbottle lens, which utilizes a dielectric microbottle lens and a conventional optical microscope to construct a super-resolution imaging method and system based on long focal depth and long working distance microparticle lenses. , capable of achieving non-contact, non-destructive and non-contamination imaging of samples below the diffraction limit.

Example

The super-resolution imaging system and imaging method mentioned above in this application are used to perform super-resolution imaging using a composite structure microbottle lens. For the specific experimental process, please refer to the above description and will not be repeated here. The experimental results are as follows.

Please refer to Figure 14, which is a comparison of the simulation results of light focusing by SiO2 microcylindrical lenses and microbottle lenses. Figures 7(a) and (b) respectively show the focused light formed by plane waves irradiating SiO2 microcylindrical lenses and SiO2 microbottle lenses. field simulation results. The diameter of the micro-cylindrical lens is 5 μm, and the parameters of the micro-bottle lens are: L=12 μm, Di=2 μm, Do=5 μm. It can be clearly seen that the focal length f = 13.3 μm and the working distance Wd = 10.8 μm of the microbottle lens are larger than the focal length f = 3.2 μm and the working distance Wd = 0.7 μm of the microcylindrical lens.

Please refer to FIG. 15 , which shows the experimental results of super-resolution imaging using a composite structure microbottle lens in this embodiment.

Figure 15(a) is a composite structure microbottle lens prepared using self-assembly and light-curing methods relying on microfibers. The material of the microfibers is silica glass, and the material of the polymer covering film is NOA 61 optical adhesive patch. The refractive index of silica glass and NOA 61 optical adhesive patch in the visible light range are 1.46 and 1.56 respectively. The parameters of the composite structure microbottle lens are: L=48.7μm, Di=13.4μm, Do=20μm. Figure 15(b) is a microscopic image of the detected sub-diffraction limit scale sample, which is a Metro Chip resolution target. The selected imaging target is a stripe structure with a line period of 300nm, in which the line width and groove width are both 150nm. It can be seen that the microscopic image obtained through a conventional optical microscope can distinguish the fringe structure with a period of 360 nm, but the fringe structures with a period of 300 nm and 260 nm cannot be distinguished. Figure 15(c) is a digital format image obtained by introducing a composite structure microbottle lens for imaging. When the 3D nano-translation stage is adjusted to the microbottle lens distance from the Metro chip sample to d=6.5μm, it can be distinguished in the microscopic image The stripe structure with a period of 300nm breaks through the resolution capability of conventional optical microscopes. Figure 15(d) is the light intensity distribution diagram of the horizontal line marked in Figure 15(c). It can be seen that the middle part of the stripe structure can be distinguished. Through the light intensity peak point, the five line distribution periods can be obtained. The lateral distance of the microscopic image is 2383nm. Compared with the actual period of the stripe structure of 300nm, it can be concluded that the magnification of the super-resolution image is approximately 1.59 times.

The above are only preferred embodiments of the present application and only specifically describe the technical principles of the present application. These descriptions are only for explaining the principles of the present application and cannot be construed as limiting the protection scope of the present application in any way. Based on the explanation here, any modifications, equivalent substitutions and improvements made within the spirit and principles of the present application, and those skilled in the art can think of other specific implementations of the present application without having to exert creative efforts. should be included in the protection scope of this application.

Claims

A composite structure microbottle lens, characterized in that the composite structure microbottle lens includes a microfiber and a covering film covering the microfiber, and the outline of the liquid covering film is a microbottle structure.
The composite structure microbottle lens according to claim 1, characterized in that the length L of the composite structure microbottle lens along the axial direction of the microfiber ranges from 12 microns to 89.2 microns; the diameter of the microfiber D i ranges from 2 microns to 13.4 microns; the maximum diameter D o of the outline of the polymer covering film in the direction perpendicular to the axial direction of the microfiber ranges from 5 microns to 38.4 microns.
The composite structure microbottle lens according to claim 1, characterized in that the ratio between the length L of the composite structure microbottle lens along the axial direction of the microfiber and the diameter D i of the microfiber is between 3.63 and within the range of 6.
The composite structure microbottle lens according to claim 1, characterized in that the length L of the composite structure microbottle lens along the axial direction of the microfiber and the outline of the polymer covering film are perpendicular to the The ratio between the maximum diameter Do of the microfiber in the axial direction is in the range of 2.32 to 2.44.
The composite structure microbottle lens according to claim 1, characterized in that the maximum diameter D o of the outline of the polymer covering film in the direction perpendicular to the axial direction of the micro optical fiber is equal to the diameter D of the micro optical fiber. The ratio between i is in the range of 1.49 to 2.5.
The composite structure microbottle lens according to claim 1, characterized in that, the length L of the composite structure microbottle lens along the axial direction of the microfiber and the outline of the polymer covering film are perpendicular to the The ratio between the maximum diameter Do of the microfiber in the axial direction and the diameter Di of the microfiber is in the range of 4 to 7.38.
The composite structure microbottle lens according to claim 1, characterized in that the thinnest part of one end of the semi-tapered microfiber and the area with the same diameter is the tapered waist area, and the area between the bare optical fiber and the tapered waist area is The area where the optical fiber diameter gradually decreases is the tapered transition area; the covering film covers the tapered waist area of the semi-tapered microfiber.
The composite structure microbottle lens according to claim 1, wherein the covering film includes a polymer covering film.
The composite structure microbottle lens according to claim 1, wherein the polymer covering film includes a UV glue covering film or a PDMS covering film.
A super-resolution imaging system based on a microbottle lens, characterized in that it includes: a microbottle lens module, a position control module and an optical microscope; the microbottle lens module includes a composite as described in any one of claims 1 to 8 Structural microbottle lenses;

The position control module can control the rotation of the microbottle lens. The sub-diffraction limit scale sample to be imaged is installed on the stage of the optical microscope. The microbottle lens is arranged on the sub-diffraction limit scale sample to be imaged. Above, the microbottle lens and the optical microscope can be used to perform super-resolution imaging of the sub-diffraction limit scale sample to be imaged.
The super-resolution imaging system based on a microbottle lens according to claim 9, wherein the microbottle lens module further includes an optical fiber handle, and both ends of the optical fiber handle are respectively connected to the composite structure microbottle lens; and the position control module.
The super-resolution imaging system based on microbottle lenses according to claim 9, characterized in that the sub-diffraction limit scale sample to be imaged is a periodic nanostructure sample, and the periodic nanostructure sample includes a grating structure. or nanodisk array structures.
A method for preparing a composite structure microbottle lens, which is characterized by including the following steps:

Two sections of semi-tapered microfibers are provided, wherein the thinnest part of one end of the semi-tapered microfiber and the area with equal diameters is the tapered waist region, and the diameter of the microfiber gradually becomes smaller between the bare optical fiber and the tapered waist region. The area is a tapered transition zone;

Transfer the polymer microliquid to one of the tapered transition zones;

Slide the polymer microfluid along the tapered transition zone to the tapered waist zone, and partially adhere to the semi-tapered microfiber;

The polymer liquid attached to the semi-tapered microfiber forms several tiny droplets;

Transfer the tiny droplets to the tapered waist of another section of the semi-tapered microfiber;

The tiny droplets transferred to the tapered waist of another section of the semi-tapered microfiber form a liquid covering film, and form a microbottle lens with a composite structure with the wrapped microfiber;

The liquid covering film is solidified to form a solid composite structure microbottle lens.
The method for preparing a composite structure microbottle lens according to claim 12, wherein the step of providing two sections of semi-tapered microfiber specifically includes the following steps:

Strip the coating layer of the middle part of the optical fiber to obtain a bare optical fiber containing the cladding and core layers;

Stretch the bare optical fiber to obtain a tapered microfiber;

The tapered microfiber is pulled off to form two sections of semi-tapered microfiber.
The method for preparing a composite structure microbottle lens according to claim 12, wherein the optical fiber is a cylindrical optical fiber.
The method for preparing a composite structure microbottle lens according to claim 12, wherein the step of stretching the bare optical fiber to obtain a tapered microfiber specifically includes: using a stepper step under heating conditions. The motor stretches the bare fiber to obtain a tapered micro-fiber.
The method for preparing a composite structure microbottle lens according to claim 12, wherein the step of transferring the polymer microliquid to the tapered transition zone of one section of the semi-tapered microfiber specifically includes the following: Step: Use a syringe to transfer the polymer microfluid to the tapered transition zone of the semi-tapered microfiber, where the polymer microfluid includes UV glue or PDMS.
The method for preparing a composite structure microbottle lens according to claim 12, wherein the polymer microliquid is slid along the conical transition area to the cone waist area, and is partially attached to the semi-cone shape. The steps on microfiber specifically include the following steps:

The semi-tapered microfiber is erected in a direction from the tapered transition area to the tapered waist area, so that the polymer droplets follow the tapered transition of the semi-tapered microfiber under the action of gravity. The region slides down to the tapered waist region and is partially attached to the semi-tapered microfiber.
The method for preparing a composite structure microbottle lens according to claim 12, wherein the step of transferring the tiny droplets to the tapered waist of another section of the semi-tapered microfiber specifically includes the following: Step: Use precision mechanical control to transfer the tiny droplets to the tapered waist of another semi-tapered microfiber.
The method for preparing a composite structure microbottle lens according to claim 12, characterized in that the tiny droplets transferred to the cone waist of another section of the semi-tapered microfiber form a liquid covering film and are combined with the wrapped In the step of forming a microbottle lens with a composite structure from a microfiber, the liquid covering film has a microbottle structure.
The method for preparing a composite structure microbottle lens according to claim 18, characterized in that one or more of the liquid covering films are transferred to the tapered waist of the microfiber.
The method for preparing a composite structure microbottle lens according to claim 12, wherein the step of solidifying the liquid covering film to form a solid composite structure microbottle lens specifically includes the following steps: curing by light or heat The liquid covering film is solidified to form a solid composite structure microbottle lens.