WO2023192995A3 - Processes and systems using for culturing cells using feeder cells in a hollow fiber cartridge - Google Patents

Processes and systems using for culturing cells using feeder cells in a hollow fiber cartridge Download PDF

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Publication number
WO2023192995A3
WO2023192995A3 PCT/US2023/065220 US2023065220W WO2023192995A3 WO 2023192995 A3 WO2023192995 A3 WO 2023192995A3 US 2023065220 W US2023065220 W US 2023065220W WO 2023192995 A3 WO2023192995 A3 WO 2023192995A3
Authority
WO
WIPO (PCT)
Prior art keywords
bioreactor
cells
hollow fiber
processes
systems
Prior art date
Application number
PCT/US2023/065220
Other languages
French (fr)
Other versions
WO2023192995A2 (en
Inventor
Manuel Alejandro TAMARGO
Bianca Melissa DREVENSEK
Original Assignee
Edge Foods Co.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Edge Foods Co. filed Critical Edge Foods Co.
Publication of WO2023192995A2 publication Critical patent/WO2023192995A2/en
Publication of WO2023192995A3 publication Critical patent/WO2023192995A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/10—Hollow fibers or tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1352—Mesenchymal stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/74—Alginate

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Immunology (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Computer Hardware Design (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

This invention provides a process for culturing eukaryotic cells, comprising culturing eukaryotic cells encapsulated in a first set of microcarriers in a culturing system comprising: (a) a bioreactor comprising: (i) an interior and interior surface; (ii) means to aerate the interior of the bioreactor; and (iii) a semi-permeable barrier which: 1. separates the interior of the bioreactor into a bottom portion and a top portion; 2. is impermeable to the first set of microcarriers; and 3. is permeable to liquids; and (b) one or more hollow fiber cartridges containing feeder cells connected to the bioreactor, preferably removably connected to the bioreactor, more preferably removably connected to the bioreactor via one or more couplings, more preferably wherein the couplings are disposable.
PCT/US2023/065220 2022-03-31 2023-03-31 Processes and systems using for culturing cells using feeder cells in a hollow fiber cartridge WO2023192995A2 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US202263325954P 2022-03-31 2022-03-31
US202263325883P 2022-03-31 2022-03-31
US63/325,954 2022-03-31
US63/325,883 2022-03-31
US202263436162P 2022-12-30 2022-12-30
US63/436,162 2022-12-30

Publications (2)

Publication Number Publication Date
WO2023192995A2 WO2023192995A2 (en) 2023-10-05
WO2023192995A3 true WO2023192995A3 (en) 2023-11-09

Family

ID=88203586

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2023/065220 WO2023192995A2 (en) 2022-03-31 2023-03-31 Processes and systems using for culturing cells using feeder cells in a hollow fiber cartridge

Country Status (1)

Country Link
WO (1) WO2023192995A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070184576A1 (en) * 2005-11-29 2007-08-09 Oregon State University Solution deposition of inorganic materials and electronic devices made comprising the inorganic materials
US20100261304A1 (en) * 2009-04-09 2010-10-14 State of Oregon acting by and through the State Board of Higher Education on behalf of Oregon Solution-based process for making inorganic materials
US20190008902A1 (en) * 2015-12-30 2019-01-10 The Regents Of The University Of California Methods for enhanced production and isolation of cell-derived vesicles

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070184576A1 (en) * 2005-11-29 2007-08-09 Oregon State University Solution deposition of inorganic materials and electronic devices made comprising the inorganic materials
US20100261304A1 (en) * 2009-04-09 2010-10-14 State of Oregon acting by and through the State Board of Higher Education on behalf of Oregon Solution-based process for making inorganic materials
US20190008902A1 (en) * 2015-12-30 2019-01-10 The Regents Of The University Of California Methods for enhanced production and isolation of cell-derived vesicles

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
INOUE YUICHI, N ARITA, K TERUYA, Y KATAKURA, S SHIRAHATA: "Efficient Production of Recombinant Human Erythropoietin by Replenishment of Microcarriers in the Hollow Fiber Culture Cassette", BIOSCI BIOTECHNOL BIOCHEM ., vol. 63, no. 9, 1 September 1999 (1999-09-01), pages 1624 - 1626, XP093109209, DOI: 10.1271/bbb.63.1624 *

Also Published As

Publication number Publication date
WO2023192995A2 (en) 2023-10-05

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