WO2023189236A1 - Procédé d'imagerie et dispositif d'imagerie - Google Patents
Procédé d'imagerie et dispositif d'imagerie Download PDFInfo
- Publication number
- WO2023189236A1 WO2023189236A1 PCT/JP2023/008334 JP2023008334W WO2023189236A1 WO 2023189236 A1 WO2023189236 A1 WO 2023189236A1 JP 2023008334 W JP2023008334 W JP 2023008334W WO 2023189236 A1 WO2023189236 A1 WO 2023189236A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- imaging
- sample
- photographing
- image
- analysis
- Prior art date
Links
- 238000003384 imaging method Methods 0.000 title claims abstract description 300
- 238000004458 analytical method Methods 0.000 claims abstract description 169
- 238000000034 method Methods 0.000 claims description 74
- 230000008859 change Effects 0.000 claims description 28
- 210000001161 mammalian embryo Anatomy 0.000 claims description 28
- 238000012014 optical coherence tomography Methods 0.000 claims description 15
- 206010028980 Neoplasm Diseases 0.000 claims description 4
- 201000011510 cancer Diseases 0.000 claims description 4
- 230000015572 biosynthetic process Effects 0.000 claims description 3
- 239000000523 sample Substances 0.000 abstract description 161
- 239000012472 biological sample Substances 0.000 abstract description 18
- 210000004027 cell Anatomy 0.000 description 65
- 230000003287 optical effect Effects 0.000 description 34
- 230000008569 process Effects 0.000 description 34
- 239000013307 optical fiber Substances 0.000 description 25
- 230000007246 mechanism Effects 0.000 description 21
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 10
- 238000010586 diagram Methods 0.000 description 9
- 238000001514 detection method Methods 0.000 description 8
- 229910002092 carbon dioxide Inorganic materials 0.000 description 5
- 239000001569 carbon dioxide Substances 0.000 description 5
- 230000006870 function Effects 0.000 description 5
- 239000012634 fragment Substances 0.000 description 4
- 210000002220 organoid Anatomy 0.000 description 4
- 238000003776 cleavage reaction Methods 0.000 description 3
- 239000004973 liquid crystal related substance Substances 0.000 description 3
- 230000007017 scission Effects 0.000 description 3
- 206010073306 Exposure to radiation Diseases 0.000 description 2
- 230000005856 abnormality Effects 0.000 description 2
- 230000005779 cell damage Effects 0.000 description 2
- 208000037887 cell injury Diseases 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000004141 dimensional analysis Methods 0.000 description 2
- 238000005562 fading Methods 0.000 description 2
- 230000001678 irradiating effect Effects 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000010191 image analysis Methods 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000000472 morula Anatomy 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000003325 tomography Methods 0.000 description 1
- 210000004340 zona pellucida Anatomy 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
Definitions
- Patent Document 1 describes a technique for acquiring a three-dimensional image of an embryo by optical coherence tomography.
- the incubator 10 is a device that accommodates a sample container 90 inside and keeps conditions such as the temperature and carbon dioxide concentration of the sample 9 constant.
- the stage 20, the light irradiation section 30, the first imaging section 40, and the second imaging section 50 are housed inside the incubator 10.
- the incubator 10 only needs to accommodate at least the sample container 90 and the stage 20 that supports the sample container 90, and the other components of the imaging device 1 may be placed outside the incubator 10.
- the focus movement mechanism 43 is a mechanism that changes the focal position of the camera 42.
- the focal point moving mechanism 43 uses an actuator such as a motor or a piezo to move the position of the objective lens 411 minutely along the optical axis. This causes the focal position of the camera 42 to move slightly in the vertical direction.
- the first imaging unit 40 photographs the sample 9 multiple times (multi-focus photography) while changing the position of the focal point using the focal point movement mechanism 43. As a result, a plurality of two-dimensional images with different focal positions are acquired.
- the "first imaging” performed in the first imaging unit 40 refers to multi-focus bright field two-dimensional imaging using an optical microscope.
- the "first image” acquired by the first imaging unit 40 refers to a plurality of two-dimensional images obtained by first imaging and having different focal positions.
- step S15 if the computer 60 determines that the first analysis result R1 of the first analysis step (S14) satisfies the first condition (step S15: Yes), the imaging control unit 61 immediately A second imaging step is performed by the second imaging unit 50 on the sample 9 that has been photographed.
- the imaging position adjustment process (step S431), the first imaging process (step S432), and the first analysis process (step S433) are the same as in the first embodiment. This is performed in the same manner as steps S12 to S14.
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Mathematical Physics (AREA)
- Theoretical Computer Science (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
La présente invention concerne un procédé d'imagerie dans lequel les étapes suivantes sont exécutées de façon répétée et périodique: une première étape (S13) d'imagerie servant à acquérir une image d'un échantillon à l'aide d'un premier procédé d'imagerie; une première étape (S14) d'analyse servant à analyser une première image acquise lors de la première étape (S13) d'imagerie; et une étape (S15) de détermination servant à déterminer si un résultat d'analyse issu de la première étape (S14) d'analyse satisfait une première condition. S'il est déterminé lors de l'étape (S15) de détermination que la première condition est satisfaite, la première étape (S13) d'imagerie, la première étape (S14) d'analyse, et l'étape (S15) de détermination sont temporairement stoppées, et une seconde étape (S17) d'imagerie servant à acquérir une image de l'échantillon à l'aide d'un second procédé d'imagerie est exécutée. En effectuant périodiquement la première étape (S13) d'imagerie et la première étape (S14) d'analyse, une variation de série chronologique dans un échantillon biologique peut être déterminée de façon appropriée, et la seconde étape (S17) d'imagerie peut être effectuée suivant une chronologie nécessaire en utilisant un procédé d'imagerie avec lequel une imagerie fréquente ou à intervalles courts est difficile. Ceci rend possible l'observation appropriée de variations de série chronologique dans l'échantillon biologique.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022055265A JP2023147636A (ja) | 2022-03-30 | 2022-03-30 | 撮影方法および撮影装置 |
| JP2022-055265 | 2022-03-30 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2023189236A1 true WO2023189236A1 (fr) | 2023-10-05 |
Family
ID=88201272
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/JP2023/008334 WO2023189236A1 (fr) | 2022-03-30 | 2023-03-06 | Procédé d'imagerie et dispositif d'imagerie |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JP2023147636A (fr) |
| WO (1) | WO2023189236A1 (fr) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2015019637A (ja) * | 2013-07-22 | 2015-02-02 | オリンパス株式会社 | サバイビンについての細胞解析方法、並びにそれを利用した癌の解析方法および薬効のスクリーニング方法 |
| JP2018093749A (ja) * | 2016-12-08 | 2018-06-21 | 大日本印刷株式会社 | 細胞品質評価システム、プログラム及び細胞品質評価方法 |
| JP2020130133A (ja) * | 2019-02-25 | 2020-08-31 | 株式会社Ｓｃｒｅｅｎホールディングス | 撮像システム |
| JP2022001983A (ja) * | 2020-06-19 | 2022-01-06 | 株式会社Ｓｃｒｅｅｎホールディングス | 画像処理方法、プログラムおよび記録媒体 |
-
2022
- 2022-03-30 JP JP2022055265A patent/JP2023147636A/ja active Pending
-
2023
- 2023-03-06 WO PCT/JP2023/008334 patent/WO2023189236A1/fr unknown
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2015019637A (ja) * | 2013-07-22 | 2015-02-02 | オリンパス株式会社 | サバイビンについての細胞解析方法、並びにそれを利用した癌の解析方法および薬効のスクリーニング方法 |
| JP2018093749A (ja) * | 2016-12-08 | 2018-06-21 | 大日本印刷株式会社 | 細胞品質評価システム、プログラム及び細胞品質評価方法 |
| JP2020130133A (ja) * | 2019-02-25 | 2020-08-31 | 株式会社Ｓｃｒｅｅｎホールディングス | 撮像システム |
| JP2022001983A (ja) * | 2020-06-19 | 2022-01-06 | 株式会社Ｓｃｒｅｅｎホールディングス | 画像処理方法、プログラムおよび記録媒体 |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2023147636A (ja) | 2023-10-13 |
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