WO2023182610A1

WO2023182610A1 - Method for mass production of fulvic acid from humus soil

Info

Publication number: WO2023182610A1
Application number: PCT/KR2022/018813
Authority: WO
Inventors: 박태철; 김홍식; 김아람
Original assignee: 주식회사 네오맥스연구소
Priority date: 2022-03-25
Filing date: 2022-11-25
Publication date: 2023-09-28
Also published as: KR20230139186A

Abstract

The present invention relates to a method for mass production of fulvic acid from humus soil. A method for extracting fulvic acid from humus soil according to the present invention employs at least one enzyme selected from the group consisting of NAD, NADP, FAD, CoA, ATP, GTP, TPP, and biotin, and a ceramic separator to activate humic matter, which is humus, whereby not only can the overall extraction time of fulvic acid be shortened, but also the extraction yield of fulvic acid is higher than when no enzymes are used. In addition, according to the present invention, fulvic acid in a state having a strong redox potential (in an inactive state) can be obtained without adding any chemical components. Therefore, having the effects of virus inactivation, antioxidative action, antiseptic, deodorizing, and anticorrosive action, detergent action, heavy metal dissolution, and harmful chemical substance dissolution and neutralization in addition to antibacterial and sterilizing actions, the fulvic acid with a strong redox potential according to the present invention is expected to be advantageously used as an activator, an antibiotic, and a supplement in the agriculture, fishery, and livestock industries.

Description

Method for mass producing fulvic acid from humus soil

The present invention relates to a method for mass producing fulvic acid from humus soil, and more specifically, an extraction method for obtaining a large amount of high-quality aqueous fulvic acid solution from humus material using enzymes and a ceramic separator. It's about.

Natural humic substances are attracting attention as excellent natural substances that have various pharmacological activities while being significantly effective in improving water quality and fish farm environment. These natural humic substances are formed as a product of erosion of organic substances in the soil. It is usually found in soil, water, sediment, coal, and peat in the natural environment.

Natural humic substances are dark-colored, amorphous complexes with a three-dimensional structure, and contain high molecular weight phenolic substances that can chelate metals and inorganic substances. Natural humic substances play an important role in connecting inorganic and organic compounds in the natural environment. It is presumed that this is because the surrounding chains of natural humic substances contain aromatic, hydroxyl, carbonyl, carboxyl, amine, and sulfhydryl groups, and thus have various properties and structures.

Natural humic substances, which are alkaline aqueous solutions, can be separated into fulvic acid and humic acid due to differences in solubility in acids. Fulvic acid is soluble in acids, but humic acid is insoluble in strong acids.

Among them, fulvic acid is a low molecular weight acid produced by the action of useful microorganisms as a part of humic substances accumulated in the soil. It has high biological activity, easily combines with minerals and elements in its molecular structure, and has excellent penetration due to its low surface tension. Fulvic acid is rich in minerals and has a strong anti-sulfur antioxidant effect. When savoring fulvic acids, astringent, bitter, and sour tastes are mentioned as characteristic tastes. Meanwhile, fulvic acid is being developed as an ingredient for food, medicine, and cosmetics. There is a problem in that fulvic acid is not easy to purify because it has the property of being soluble in all acidic, neutral, and basic solutions.

However, fulvic acid can generally be purified with high purity through a strong base/strong acid treatment extraction method specified by the International Humic Substances Society (IHSS), an international academic society. Extraction and purification methods (strong base/strong acid treatment methods, etc.) of these fulvic acids are well established in Korea, and these details are detailed in Korean Patent Application Nos. 10-2011-0027154 and 10-2011-0027117. .

Conventional fulvic acid extraction uses an alkaline-acid extraction method. When the humic substances are precipitated using an aqueous solution of sodium hydroxide (NaOH) at pH 13.5 for 4 to 48 hours, they are separated into precipitate and supernatant. The separated supernatant was again acidified to pH 0.5 using hydrochloric acid (HCl) and allowed to precipitate again for about 12 hours. Accordingly, humic acid precipitates, and the supernatant may be composed of fulvic acid.

On the other hand, humic substances (Humic Substances, or Humus) are formed as the components of dead bodies such as wetlands, swamps, lake bottoms, plants, algae, and seaweed undergo pressure and temperature changes over hundreds to tens of millions of years, causing bacteria, etc. It exists in humus soil created through decomposition/polymerization by microorganisms, and most of it is a collection of organic acids, namely humic acid, fulvic acid, and minerals. Fulvic acid, which exists in smaller amounts among these organic acids, is a humic substance that is highly soluble in water regardless of pH concentration and has a (dark) brown or light blackish brown color. Additionally, these corrosive substances have already been decomposed by an astronomical number of microorganisms during the process of their composition. In this way, natural humic acid or fulvic acid can be collected and extracted anywhere where corrosion has progressed to a certain degree, such as water, soil, peat, and sedimentary layers. In addition, fulvic acid can be obtained by fermenting tangerines or plants to artificially reproduce the 'humic acid process' and generate humic acid.

Fermented, matured, purified various minerals, amino acids, vitamins, enzymes, sulfur, iodine, and chilating fulvic acid complexes present in humic substances have the power to provide energy while maintaining biological balance. , are the world's most sophisticated natural organic electrolytes (powerful organic electrolytes). Scientists who studied the properties of electrolytes in amoeba as animal cells confirmed through experiments that the vitality of cells was revived through these electrolytes, and reported that it was a surprising and beautiful phenomenon.

Humic substances are electrolyte mixtures with various organic compound structures, and their chemical molecular structures are not constant because they vary depending on the country's topography and geological environment. Humus has a high affinity for metal ions exposed to the soil environment and forms a humic colloid that acts like a soluble ion exchange material, and the distribution and behavior of metal ions in soil particles and soil water (Migration behavior) ) plays an important role in These humic substances are divided into Fulvic Acid (FA), Humic Acid (HA), and Humin, depending on the difference in molecular weight and solubility depending on pH in aqueous solution. It has very diverse compositions and physical and chemical properties depending on the vegetation, climate, geological characteristics, and soil depth of the area where it was formed. Humic substances are distributed in many countries such as Russia, Canada, the United States, India, China, and Japan, but substances containing a lot of fulvic acid are believed to be mainly distributed in islands and swamps such as the Philippines, Southeast Asia, and Japan. The areas where humic substances are discovered in Korea are Gangneung, Pyeongtaek, the lower reaches of Hallasan Mountain, Goesan, and it is known that humic substances are distributed in geological layers located within 10km from the coastline near the southwestern coast.

Humic substances or humus are not only structurally unstable, but are also substances that have existed in water for a considerable period of time without being well decomposed by microorganisms. McCarthy, an American corrosive material researcher, commented on the characteristics of corrosive materials that do not decompose easily, saying, 'The structure of the central (nucleus) part that determines the properties of corrosive materials does not change, but the outer structure of the materials undergoes a decomposition process. Because this changes slowly, it takes quite a long time to decompose.'

Accordingly, the present inventors completed the present invention by extracting a large amount of aqueous fulvic acid solution, which is a natural substance derived from humic substances and has strong reducing/oxidizing power, through the promoting action of enzymes.

The technical problem to be solved by the present invention is to provide a method for extracting a large amount of natural fulvic acid aqueous solution with strong reducing/oxidizing power from corrosive substances.

In order to solve the above technical problems, the present invention provides a method for extracting a large amount of natural fulvic acid aqueous solution from humic substances, comprising the following steps:

(S1) adding humus to water to prepare a mixed suspension while maintaining pH 6.0-8.0 and temperature 36-44°C;

(S2) fermentation by adding at least one enzyme selected from the group consisting of NAD, NADP, FAD, CoA, ATP, GTP, TPP and biotin to the mixed suspension prepared in (S1);

(S3) First separation and extraction of the supernatant of the fermentation broth fermented in step (S2), removal of sedimented and precipitated corrosive material sludge that has settled on the bottom, and secondary separation using a hydraulic press or centrifuge. Then, extracting and recovering the filtrate from the sludge and combining it with the first extraction supernatant;

(S4) maturing fulvic acid by injecting oxygen-containing bubbles from the ceramic filter membrane of the aerobic tank while maintaining the temperature of the supernatant obtained in step (S3) at 11 to 16°C;

(S5) removing fine residual floating matter from the aged liquid aged in step (S4) using a ceramic separator; and

(S6) A step of producing fulvic acid by subjecting the aged liquid from which residual suspended matter was removed in step (S5) to antibacterial and sterilization filtration using a ceramic filter of 0.01 to 0.5 μm.

Preferably, in step (S1), 150 to 300 kg of humus soil is added to 500 to 800 L of water.

Preferably, the water in step (S1) is groundwater or purified water.

The groundwater or purified water is characterized in that the chlorine component has been removed.

The chlorine removal is characterized by using activated carbon or ion exchange resin adsorbent.

Preferably, in step (S2), the enzyme is added in an amount of 1,500 to 3,000 mg per 1 kg of humus soil.

Preferably, in the step (S2), a ceramic filter of 0.01 to 0.5 μm is used to generate bubbles of 100 to 200 μm in size to facilitate oxygen supply.

Preferably, in step (S2), the water temperature is maintained at 36-44°C.

Preferably, in step (S3), the supernatant is separated by injecting air from the outside from bottom to top through ultra-fine ceramic pores of 0.01-0.5 ㎛ to generate ultra-fine oxygen bubbles of 100-200 ㎛ in the suspension. It is characterized in that the supernatant is separated by inducing corrosive substances in the suspension to float.

Preferably, the process further includes the step of drying the humus sludge remaining after the secondary extraction in step (S3) to produce humus powder with a moisture content of 18 to 22%.

The humus powder is used as a rooting agent to help the roots of seedlings grow well in agriculture, as an additive/supplement to antibiotic feed for livestock/livestock, or as a natural antibiotic feed for farmed fish in the fisheries industry.

Preferably, in step (S4), the aging process is performed for 70 to 90 days while generating oxygen bubbles.

Preferably, in step (S6), antibacterial and sterilizing filtration are performed while stirring the aged liquid.

Preferably, the fulvic acid obtained in step (S6) is further subjected to a low-temperature sterilization process.

The pasteurization is characterized by maintaining the temperature of the aqueous solution at 63°C for 30 minutes and then gradually cooling it.

As such, the method of extracting fulvic acid from humus soil according to the present invention removes humic substances, which are humus soil, by using at least one enzyme selected from the group consisting of NAD, NADP, FAD, CoA, ATP, GTP, TPP, and biotin and a ceramic separator. Not only can activation shorten the overall extraction time of fulvic acid, but it also has the advantage of increasing the extraction yield of fulvic acid compared to when the enzyme is not used. In addition, according to the present invention, fulvic acid in a state with strong reduction-oxidation power (inert) can be obtained without adding any chemical components. Therefore, according to the present invention, it is a material with strong reduction and oxidation power, and has effects such as virus inactivation, antioxidant effect, preservative and deodorizing action, surfactant action, heavy metal dissolution action, and harmful chemical substance dissolution and neutralization action in addition to antibacterial and sterilizing action. It is expected to be very useful as an activator, antioxidant, and supplement in the fields of agriculture, fisheries, and livestock farming.

The following drawings attached to this specification illustrate preferred embodiments of the present invention, and serve to further understand the technical idea of the present invention along with the contents of the above-described invention. Therefore, the present invention is limited to the matters described in such drawings. It should not be interpreted in a limited way.

Figure 1 is a water tank diagram showing a method for extracting natural material fulvic acid as one of the embodiments of the present invention, showing the raw material input and dilution step, enzyme addition step, floating material flotation and stirring step, supernatant extraction step, and fulvic acid extraction step. Here, (a) it is a process of floating and agitating suspended matter by installing a ceramic separator and generating bubbles here, and (b) a conceptual diagram of the process of extracting natural product fulvic acid by turning the drain tap in the order from the bottom of the top to the bottom. am.

Figure 2 shows the process in which a suspension is made from humic substances, fermented and aged, and minerals are formed on the surface of the supernatant. Calcium, sulfur, etc. are condensing on the surface of the water tank from the initial stage (a) through the intermediate stage (b) to stage (c). The fermentation process is complete when a dark gray film is formed on the surface of the supernatant, as shown in (c).

In Figure 3(a), when the suspension is left to stand after fermentation and maturation, the suspended fine particles of the mineral film formed in Figure 2(c) settle to the bottom. This is a photo taken of the surface of the sludge accumulated on the bottom of the tank after extracting the supernatant. It contains a lot of moisture and is dark brown in color.

Figure 3(b) is a photograph showing the surface and vertical section (lower layer of the yellow surface) where minerals and sulfur components (yellow) are clearly revealed as moisture evaporates.

In Figure 3(c), the yellow surface changed to bright yellow as most of the moisture evaporated, and the dark gray lower vertical layer also became light gray as drying progressed.

Figure 4 shows the process of levitating suspended matter in the supernatant and stirring and maturing, where a white mineral saponin bubble film (a) begins to form thinly on the surface of the water. After a certain period of time, these bubbles begin to gather together (b) and disappear after a gradual disappearance stage (c).

In Figure 5(a), the part that looks like a curved pipe at the bottom or the eaves at the end of a slate roof is a ceramic separator. It shows ultrafine bubbles floating through the pores of the ceramic separator.

Figure 5(b) shows ultrafine bubbles floating and combining with floating sediment particles to form a brown suspended particle film (layer) on the water surface, and the side is a transparent polycarbonate tank. This is a photo taken from an angle viewed from above.

Figure 6 shows an aqueous solution of fulvic acid (NEOMAX), a natural substance, that was separated and extracted from the supernatant, subjected to final filtration processes, and then placed in a transparent container and a brown container, respectively.

Figure 7(a) shows the growth of garlic roots grown for more than 3 weeks with fulvic acid (Neomax) stock solution diluted 750:1 to 500:1.

Figure 7(b) shows that the roots of garlic were finely developed in all directions as a result of cultivating garlic with the diluted solution.

In Figure 7(c), if you zoom in and look closely at the garlic root (b), you can see parts of different colors, such as brown dots, in the middle of the root in addition to the tip of the root. This indicates that nutrients are absorbed through the cells there. see.

Figure 8(a) shows a dark purple stem obtained by growing 1-year-old ginseng seedlings in hydroponic cultivation for 30 to 35 days with Neomax diluted solution (diluted stock solution 750:1 to 500:1), showing saponin Re. It is ginseng in which the main ingredient is expressed.

Figure 8(b) shows ginseng with the main component saponin F1 expressed, forming dark purple stems by diluting fulvic acid Neomax stock solution to 750:1 to 500:1 and growing 5-year-old ginseng seedlings in hydroponics for 45 days. am.

Figure 9(a) shows the results obtained by diluting Neomax stock solution from 750:1 to 500:1 and spraying the leaves four times when cultivating sweet potatoes, resulting in mass production of more than 2.5 times the normal harvest amount.

Figure 9(b) shows that as a result of hydroponic cultivation of sweet potatoes for a total of 80 days (one month longer than usual), several short and large leaf stems were formed, like potato leaf stems.

Figure 9(c) shows that humic material sludge (hereinafter referred to as MM soil), which precipitated during the process of extracting Neomax crude solution, was used for pepper cultivation. 5 g per head of pepper was mixed into the soil and watched as the peppers grew in pesticide-free conditions. As a result, the stems of each pepper grew to an average of more than 2.5 m and reached the ceiling of the greenhouse.

Hereinafter, the present invention will be described in more detail.

The present invention provides a method for extracting a large amount of natural fulvic acid aqueous solution from humic substances, comprising the following steps:

(S6) A step of producing fulvic acid as transparent natural drinking water by subjecting the aged liquid from which residual suspended matter has been removed in step (S5) to antibacterial and sterilization filtration using a ceramic filter of 0.01 to 0.5 μm.

According to the present invention, fulvic acid, a natural substance, can be easily extracted from humic substances through an activated fermentation process using enzymes and a ceramic membrane, and by using a simple, inexpensive ceramic membrane in the manufacturing process, the precipitate is converted into a pure aqueous solution. It is possible to produce in large quantities an aqueous solution of fulvic acid, a natural substance that does not occur at all, has a good water content and has strong reducing and oxidizing power.

In the method for producing fulvic acid of the present invention, step (S1) is characterized by adding humus to water to prepare a mixed suspension while maintaining pH 6.0 to 8.0 and temperature 36 to 44 ° C.

In the fulvic acid extraction method, first, a ceramic filter membrane with a bubble supply device is installed on the bottom of the water tank containing the suspension. Here, pour about 500 to 800 L of groundwater or purified water (pH 6.0 to 8.0) into the water tank (1 ton capacity). In all cases, the raw water used for extraction must be purified water from which chlorine has been removed. A common method of removing chlorine components is to use adsorbents such as activated carbon or ion exchange resin. After removing chlorine in this way, the water is allowed to stand at room temperature for 3 to 5 days before proceeding with the next process.

After filling about 500 to 800 L of water prepared as above into the tank, slowly add 150 to 300 kg of humus soil to the water in the tank. The temperature of the indoor workplace is measured in a space 1.5m above the workshop floor, and the indoor temperature is maintained at 35-45℃.

In the method for producing fulvic acid of the present invention, step (S2) is fermented by adding one or more enzymes selected from the group consisting of NAD, NADP, FAD, CoA, ATP, GTP, TPP and biotin to the mixed suspension prepared above. As a step, using a 0.01-0.5㎛ ceramic filter membrane to generate bubbles of 100-200㎛ in size to facilitate oxygen supply, float the suspended matter and stir intermittently for 4-5 hours a day for 3 weeks. Let stand for 13 to 15 days to completely settle suspended fine particles. It is characterized in that it generates fine bubbles from the ceramic filter membrane and stirs and levitates the suspension to facilitate aerobic oxygen supply and promote fermentation.

After adding the one or more enzymes, the water temperature in the tank is maintained at 36 to 44°C, preferably 38 to 42°C, more preferably 40°C, so that the enzymes are well activated in the water tank, and the pH is 5.5 to 5.5°C. It is characterized by maintaining it at 7.5. It is advisable to allow a rest period in the fermentation process by drastically lowering the temperature of the workplace and supplying and ventilating fresh air so that the water temperature in the water tank reaches 25-27℃ at an appropriate time during the process. Afterwards, the temperature of the water in the water tank is raised to 36~44℃ to activate the enzyme again, and the initial working conditions are continuously maintained.

If the temperature is less than 36°C, the decomposition enzyme in the humus suspension is not activated well, and if the temperature is above 44°C, there is a problem in that the decomposition enzyme is denatured by heat and its activity is reduced.

In the case of liquids, the concentration of the enzyme is usually expressed as the number of enzyme units in 1 ml. When solid enzyme(s) are used as in the present invention, the appropriate dosage of enzyme(s) is 1,500 to 3,000 mg per 1 kg of humus soil. It is efficient not to exceed this.

Since the above humic substances are already mixed with a large amount of various types of naturally occurring microorganisms, such as Bacillus subtilis, photosynthetic bacteria, filamentous bacteria, Bacillus bacteria, algae, yeast, etc., a suspension as a mixture is used. It is not absolutely necessary to add additional microorganisms, etc. However, in order to more efficiently extract fulvic acid from the supernatant by fermenting the suspension, a specific enzyme is added, and the enzyme, a type of protein, acts as a catalyst to activate and promote the overall fermentation reaction.

When one or more of the enzymes listed above is selected and added to the suspension, most enzyme(s) vary the speed of catalysis depending on the concentration of the substrate. Enzyme(s) are generally proteins with a molecular weight of 15,000 to several million Daltons, and catalytic reactions by enzymes have high activity at low temperatures, low pressure, and near neutral pH.

At this time, reaction temperature and pH have a great influence on the activity of the enzyme. Although there are exceptions such as heat-resistant microorganisms found in hot spring areas, the activity of the enzymes mentioned above usually increases with increasing temperature up to about 40-44℃, but above that, denaturation due to heat occurs and most of them lose their activity. In addition, there are cases where the optimal pH is strongly acidic around 2, such as pepsin that acts in the stomach, but the enzyme activity in the present invention has a neutral pH (5.5 to 7.5), as in the case of most other enzymes. It appears that the vicinity is most suitable for fermentation of humic substances.

The above enzyme(s) play a catalyst role in all biochemical reactions involving proteins, metal ions, minerals, amino acids, etc. The activity of the enzyme(s) in humic substances is maximized at 36-44℃, so when the temperature rises beyond this optimal temperature range, the enzyme(s) are denatured by heat and the activity decreases.

The enzyme(s) plays the role of a catalyst, lowering the activation energy of the reaction by combining with the substrate to form an enzyme-substrate complex. In order for certain substances to come together and have a chemical reaction, these substances must have enough energy to combine or collide with each other, and the minimum energy required to achieve this state is called the activation energy of the substance. Enzymes lower this activation energy. , promotes chemical reactions within living organisms.

In the method for producing fulvic acid of the present invention, step (S3) is to first separate and extract the supernatant of the fermented broth, and remove the sedimented and precipitated corrosive material sludge that has settled on the bottom, using a hydraulic press or After secondary separation using a centrifuge, the filtrate is extracted and recovered from the sludge and combined with the first extraction supernatant.

In the above process, the Solid-Liquid Separation method involves installing a ceramic filter membrane on the bottom of the water tank and allowing air to float from the outside, which allows air from the outside to flow from bottom to top through ultra-fine ceramic pores of 0.01 to 0.5 ㎛. This is a method of levitating corrosive substances in the suspension by injecting them and inducing the generation of ultra-fine oxygen bubbles of 100 to 200㎛ in the suspension. If proper agitation is performed during this process, the microorganisms are activated and the desired supernatant can be obtained in a short period of time.

While supplying air bubbles through the ceramic filter separator installed in the water tank, the process of stirring and levitating suspended matter is repeated 3 to 5 times for 4 to 5 hours a day, and this state is maintained for a total of 20 to 30 days. I order it. When observing the supernatant while repeatedly performing the stirring and suspension flotation process, a dark brown color begins to appear on the water surface, and then a gray film, which is a mineral component, is formed on the surface of the supernatant, and the pH value of the solution increases. This drops to 2.3~2.9. This is the mature stage of the first process, and the Oxidation Reduction Potential (ORP) value is in the range of 500 to 700 mV.

At the beginning of the extraction process, the pH value of the suspension was 5.0 to 6.0, but when the extraction progresses as described above and the pH value drops to about 2.3 to 2.9, the primary supernatant from the water tank is transferred to a pump and the supernatant is collected from the ceramic membrane tank.

Next, after the supernatant is collected, sludge that settles on the bottom remains. This is secondarily extracted using a hydraulic oil pump or a centrifugal separator in the form of a barrel washing machine to extract the aqueous solution contained therein. In this way, the moisture content of the remaining corrosive sludge after secondary extraction from the settled sludge is approximately 26 to 35%.

The humus soil (called MM soil, moisture content of 18-22%) made by drying the humus sludge generated at this time is used as an additive to rooting agents in agriculture to help the roots of seedlings grow well, and as an antibiotic for livestock/livestock use. As an additive/supplement to feed, it can be used in the fisheries industry as a natural antibiotic feed for farmed fish.

In the method for producing fulvic acid of the present invention, step (S4) maintains the temperature of the supernatant obtained in step (S3) at 11 to 16°C and injects oxygen-containing bubbles from the ceramic filter membrane of the aerobic tank to produce fulvic acid. This is the stage of maturing.

The supernatant extracted through the primary filtration above is placed in a separate 1-ton capacity water tank with a ceramic filter separator installed on the bottom while maintaining a temperature of 11 to 16°C in a well-ventilated room in the dark. Pour it.

In the method for producing fulvic acid of the present invention, step (S5) is a step of removing fine residual suspended matter from the aged liquid by floating it using a ceramic separator.

The supernatant is poured into a water tank, and then the separation membrane is operated to supply oxygen bubbles to the supernatant and stirred to ensure proper maturation to float and remove fine insoluble impurities.

When the aging process is carried out for 70 to 90 days while generating ultra-fine oxygen bubbles with a ceramic filter separator until a protein-mineral film is formed on the upper surface of the supernatant, a bright film is formed on the surface of the supernatant and then disappears.

In the method for producing fulvic acid of the present invention, in step (S6), the aged liquid from which residual suspended solids have been removed is subjected to antibacterial and sterilization filtration using a ceramic filter of 0.01 to 0.5 ㎛ to produce fulvic acid as transparent natural drinking water. This is the step.

As the final stage of maturation, the fulvic acid solution is aged for 1 to 3 years while maintaining the internal temperature at 8 to 15°C for long-term storage using a warehouse-type underground space, an abandoned mine tunnel, or an unused road tunnel. , the natural substance fulvic acid can be obtained by subjecting the fulvic acid aqueous solution that has reached the mature stage to antibacterial and sterilization filtration through a 0.01-0.5㎛ membrane filter.

When air is injected into the suspension through the ceramic filter separator, the floating ultrafine bubbles collide with and adhere to the settled/suspended particles (Flocs) in the corrosive material, thereby reducing the specific gravity of the combination between Flocs and bubbles. It floats well on the surface of the water. In addition, while stirring, contaminants are filtered out from the suspension through the pores of an ultra-fine filter of about 0.01 to 0.5 ㎛. Once the supernatant is formed, it is aged at a certain temperature for a certain period of time and then the completed fulvic acid is processed. Can be extracted efficiently.

The fulvic acid extracted in this way is transferred to a vertical rectangular water tank with two outlet valves attached in a vertical direction at the lower part of the tank. A ceramic filter separator is installed on the bottom of the water tank and air is injected through it to float and remove ultrafine impurities floating in the fulvic acid. Then, the valves mounted on the water tank are sequentially opened from top to bottom. Put it in another container and store it in a cold storage.

According to one embodiment of the present invention, when using the fulvic acid aqueous solution as drinking water, a low-temperature sterilization process is additionally performed. This low temperature long time sterilization (LTLT) is a process in which the temperature of the aqueous solution is first maintained at 63°C for 30 minutes and then gradually cooled. One thing to note is that in the low-temperature sterilization process, the air in contact with the solution must be vented out before the process begins. In general, acidity is a major criterion that determines the range of survival of bacterial organisms, and most toxic bacteria are unable to function in strong acid solutions below pH 4.5.

Fulvic acid Neomax acts on taste cells to produce an astringent (astringent) taste, and this taste is known to be caused by tannin. Representative tannin components that provide an astringent taste include flavonoids and catechins. These components strengthen human capillaries and are effective in preventing high blood pressure, arteriosclerosis, and stroke. Among these tannin ingredients, catechin is known to have an antioxidant effect that is more than 50 times higher than that of vitamin C or vitamin E. These antioxidant substances have the function of removing free radicals in the body. Therefore, if you consistently drink fulvic acid, which contains a lot of vitamins, flavonoids, minerals, and amino acids, it is beneficial and effective for physical health and disease prevention, including delaying aging through antioxidants.

The fulvic acid produced in the present invention is a dark brown or brown aqueous solution depending on the raw material and its properties, the pH value was 2.2 to 2.8 at 25°C, and the specific gravity was 1.00 to 1.03. In addition, E. coli, general bacteria, and heavy metals are not detected in the aqueous solution, and the aqueous solution has a very strong and astringent taste when expressed sensuously and appreciatively, but also has some sweetness. The total solid content in the solution is 0.85~1.25%, of which The content of pure fulvic acid was 0.30-0.55% of the total weight of the aqueous solution.

According to one embodiment of the present invention, as a result of a sensory test on the aqueous solution of fulvic acid extracted in the present invention, it is a bright and pale brass-colored aqueous solution that hardly generates any precipitate, and remains in a stable state without any change even when stored for a long period of time. Maintaining.

In addition, the taste can be described as an aqueous solution of fulvic acid, a natural substance that is soft and refreshing and easy to drink, with a sour taste, an astringent taste, and a subtle sweet taste.

According to one embodiment of the present invention, for comparison, a stock solution of fulvic acid aqueous solution named NEOMAX (brand name) was added to 0.2-0.3% ginseng enzyme fermentation broth and fermented, resulting in fermentation, which normally took about 3 weeks. It was confirmed that fermentation was completed in about 7 days. Additionally, in the case of the beer fermentation process, the fermentation period was shown to be shorter by more than 45 days compared to the existing process.

According to one embodiment of the present invention, as an alternative to the antibiotic resistance problem, an aqueous solution of fulvic acid consisting only of natural substances was administered to animals as drinking water for 18 to 21 days for the purpose of using it as a livestock feed additive. As a result, it was observed that the fur of chickens and pets became shiny, their behavior became docile, and the foul odor generated from feces was reduced very effectively.

According to one embodiment of the present invention, an aqueous solution of fulvic acid diluted at 750:1 to 500:1 was administered to housed laying hens as drinking water from the beginning, and immediately before laying eggs began, 500:1 to 300: Fulvic acid drinking water was prepared and fed at a dilution ratio of 1. After one month, we carefully observed the movements of the 4,000 chickens being raised in the cage, and found that despite the intrusion of strangers, the chickens not only responded docilely, but even approached people and remained silent for quite some time. Judging by the fact that it was looking at the intruder for a long time, it was found that it was being raised in a comfortable condition without stress.

According to one embodiment of the present invention, even when the activity to collect eggs was carried out, the chickens responded calmly without being agitated, and it was reported that the workers did not notice any unpleasant odors such as excrement while collecting eggs for a long time in the cage. . When observing the laid eggs, the shells were thick and there was no fishy smell. As a result of requesting the Chungnam National University Agricultural Technology Center to analyze the composition of eggs, the nutrients were vitamin C 8.78mg/kg, tannin 6.22mg/100g, vitamin D3 9,295.35 IU/kg, polyphenol 16.86mg/100g, flavonoid 1.75mg/100g. , a value such as 1.931.11mg/kg of sulfur was confirmed.

According to one embodiment of the present invention, Neomax (fulvic acid aqueous solution) was diluted at a ratio of 750:1 to 500:1, and then supplied as a nutrient to one-year-old ginseng seedlings during hydroponic cultivation for one month. . 30 days after the start of cultivation, the stems of 1-year-old ginseng developed into a dark purple color. (Generally, the stem of ginseng has a light green color during the early growth period.) It is generally known that 30% to 35% of Re, the main component of ginseng, is found in fruits older than 5 years old as a result of component tests, but in the case of the present invention, 1-year-old roots are used. As a result of cultivating ginseng seedlings hydroponically for one month and then requesting a composition analysis in fresh ginseng form from the Korea Research Institute of Food Science, it was confirmed that Re was present at 50.3%, that is, as the main ingredient.

According to one embodiment of the present invention, when a person drinks Neomax, an aqueous fulvic acid solution, it enters the body and reaches the intestines through the stomach. Due to the action of organic acids present in Neomax, beneficial bacteria increase and harmful bacteria decrease in the intestines, making the intestinal flora sound and healthy. As a result, the odor of stool, etc. is weakened (deodorizing effect) and, depending on the person, the odor component from the blood is reduced, thereby reducing body odor and bad breath. We were able to confirm common reports from drinkers that when the intestinal flora becomes healthy, the intestinal function becomes more active in a chain, improving the efficiency of nutrient absorption from ingested food, improving constipation, and reducing hangovers.

As such, the method of extracting fulvic acid from humus soil according to the present invention removes humic substances, which are humus soil, by using at least one enzyme selected from the group consisting of NAD, NADP, FAD, CoA, ATP, GTP, TPP, and biotin and a ceramic separator. Not only can activation shorten the overall extraction time of fulvic acid, but it also has the advantage of increasing the extraction yield of fulvic acid compared to when the enzyme is not used. In addition, according to the present invention, fulvic acid in a strong (inactive) redox state can be obtained without adding any chemical components. Therefore, according to the present invention, it is a material with strong oxidation-reduction power, and has effects such as virus inactivation, antioxidant effect, preservative and deodorizing effect, surfactant activity, heavy metal dissolution effect, and harmful chemical substance dissolution and neutralization effect in addition to antibacterial and sterilizing effect. It is expected to be very useful as an activator, antioxidant, and supplement in the fields of agriculture, fisheries, and livestock farming.

As the specific parts of the present invention have been described in detail above, it is clear to those skilled in the art that these specific techniques are merely preferred embodiments and do not limit the scope of the present invention. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims

A method for extracting a large amount of natural fulvic acid aqueous solution from humic substances, comprising the following steps:

(S1) adding humus to water to prepare a mixed suspension while maintaining pH 6.0-8.0 and temperature 36-44°C;

(S2) fermentation by adding at least one enzyme selected from the group consisting of NAD, NADP, FAD, CoA, ATP, GTP, TPP and biotin to the mixed suspension prepared in (S1);

(S3) First separation and extraction of the supernatant of the fermentation broth fermented in step (S2), removal of sedimented and precipitated corrosive material sludge that has settled on the bottom, and secondary separation using a hydraulic press or centrifuge. Then, extracting and recovering the filtrate from the sludge and combining it with the first extraction supernatant;

(S4) maturing fulvic acid by injecting oxygen-containing bubbles from the ceramic filter membrane of the aerobic tank while maintaining the temperature of the supernatant obtained in step (S3) at 11 to 16°C;

(S5) removing fine residual floating matter from the aged liquid aged in step (S4) using a ceramic separator; and

(S6) A step of producing fulvic acid by subjecting the aged liquid from which residual suspended matter was removed in step (S5) to antibacterial and sterilization filtration using a ceramic filter of 0.01 to 0.5 μm.
According to claim 1,

A method for extracting a large amount of natural fulvic acid aqueous solution from humic substances, characterized in that 150 to 300 kg of humus soil is added to 500 to 800 L of water in the step (S1).
According to claim 1,

In step (S1), the water is groundwater or purified water. A method for extracting a large amount of natural fulvic acid aqueous solution from a humic substance.
According to claim 3,

A method of extracting a large amount of natural fulvic acid aqueous solution from a corrosive material, wherein the groundwater or purified water has the chlorine component removed.
According to claim 4,

A method of extracting a large amount of natural fulvic acid aqueous solution from a corrosive material, characterized in that the chlorine component is removed using activated carbon or an ion exchange resin adsorbent.
According to claim 1,

In the step (S2), the enzyme is added in an amount of 1,500 to 3,000 mg per 1 kg of humus soil. A method of extracting a large amount of natural fulvic acid aqueous solution from humus material.
According to claim 1,

A method of extracting a large amount of natural fulvic acid aqueous solution from a corrosive material, characterized in that in the step (S2), a ceramic filter membrane of 0.01 to 0.5 ㎛ is used to generate bubbles of 100 to 200 ㎛ in size to facilitate oxygen supply.
According to claim 1,

A method of extracting a large amount of natural fulvic acid aqueous solution from a humic substance, characterized in that the water temperature is maintained at 36-44°C and pH 5.5-7.5 in step (S2).
According to claim 1,

In the step (S3), the supernatant is separated by injecting air from the outside from bottom to top through ultra-fine ceramic pores of 0.01-0.5 ㎛, thereby inducing the generation of ultra-fine oxygen bubbles of 100-200 ㎛ in the suspension. A method of extracting a large amount of natural fulvic acid aqueous solution from corrosive substances, characterized in that the supernatant is separated by floating the corrosive substances in water.
According to claim 1,

A large amount of natural fulvic acid aqueous solution is produced from the humus material, characterized in that it further comprises the step of drying the remaining humus sludge after the secondary extraction in step (S3) to produce humus powder with a moisture content of 18 to 22%. How to extract it.
According to claim 10,

The humus powder is used as a rooting agent to help the roots of seedlings grow well in agriculture, as an additive/supplement to antibiotic feed for livestock/livestock, or as a natural antibiotic feed for farmed fish in the fisheries industry. Method for extracting large quantities of aqueous acid solution.
According to claim 1,

A method of extracting a large amount of natural fulvic acid aqueous solution from a humic substance, characterized in that the aging process is carried out for 70 to 90 days while generating oxygen bubbles in the step (S4).
According to claim 1,

A method for extracting a large amount of natural fulvic acid aqueous solution from a humic substance, characterized in that antibacterial and sterilizing filtration are performed while stirring the aged solution in the step (S6).
According to claim 1,

A method for extracting a large amount of natural fulvic acid aqueous solution from a humic substance, characterized in that the fulvic acid obtained in step (S6) is further subjected to a low-temperature sterilization process.
According to claim 14,

The pasteurization is a method of extracting a large amount of natural fulvic acid aqueous solution from a corrosive material, characterized in that the temperature of the aqueous solution is maintained at 63° C. for 30 minutes and then slowly cooled.
A natural fulvic acid aqueous solution prepared by a method according to any one of claims 1 to 15.