WO2023169239A1 - Dispositif de stockage d'appareil de cryoconservation à basse température d'échantillon biologique - Google Patents

Dispositif de stockage d'appareil de cryoconservation à basse température d'échantillon biologique Download PDF

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Publication number
WO2023169239A1
WO2023169239A1 PCT/CN2023/078339 CN2023078339W WO2023169239A1 WO 2023169239 A1 WO2023169239 A1 WO 2023169239A1 CN 2023078339 W CN2023078339 W CN 2023078339W WO 2023169239 A1 WO2023169239 A1 WO 2023169239A1
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WIPO (PCT)
Prior art keywords
tank
storage
liquid nitrogen
liquid
wall
Prior art date
Application number
PCT/CN2023/078339
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English (en)
Chinese (zh)
Inventor
瞿建国
刘亚飞
尹灶发
Original Assignee
上海原能细胞生物低温设备有限公司
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Application filed by 上海原能细胞生物低温设备有限公司 filed Critical 上海原能细胞生物低温设备有限公司
Publication of WO2023169239A1 publication Critical patent/WO2023169239A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
    • B65D25/02—Internal fittings
    • B65D25/10—Devices to locate articles in containers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
    • B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
    • B65D81/20—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient under vacuum or superatmospheric pressure, or in a special atmosphere, e.g. of inert gas
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W90/00—Enabling technologies or technologies with a potential or indirect contribution to greenhouse gas [GHG] emissions mitigation
    • Y02W90/10—Bio-packaging, e.g. packing containers made from renewable resources or bio-plastics

Definitions

  • the utility model relates to the technical field of low-temperature freezing of biological samples, in particular to a storage device of a low-temperature freezing device for biological samples.
  • liquid nitrogen tanks as one of the storage devices of cryogenic cryopreservation devices for biological samples, are widely used in hospitals and laboratories, and are mainly used for cryopreservation of biological materials such as cells and tissues.
  • the structure of the traditional liquid nitrogen tank is a welded double-layer vacuum tank. Inside the tank are cryovials for storing extracted biological samples. The cryovials are completely or partially immersed in liquid nitrogen for cryopreservation.
  • a known approach is to use a third tank inside the existing double-layer vacuum tank.
  • the third tank is also a vacuum chamber, so that the tank has
  • the two-layer vacuum chamber reduces the volatilization of liquid nitrogen to a certain extent and improves the thermal insulation and cold insulation effect of the liquid nitrogen tank, it is more difficult to operate in practice, and some storage equipment has limited space. Adding a chamber This will bring about the disadvantage of reducing the space for storing samples, so it is necessary to change the thinking and increase the liquid nitrogen storage capacity based on the existing space, which is a better solution.
  • the present invention provides a storage device for a biological sample low-temperature freezing device, which can make full use of the free space of the existing equipment, increase the liquid nitrogen storage capacity, and improve the depth of sample storage. Low temperature durability in low temperature environments.
  • the technical solution adopted by the utility model to solve the technical problem is: including a liquid nitrogen tank body, the liquid nitrogen tank body is provided with a liquid nitrogen storage chamber, and the liquid storage chamber is formed on the inner wall of the liquid nitrogen tank body. .
  • the liquid storage chamber is an annular hollow chamber provided on the inner wall of the liquid nitrogen tank.
  • the liquid storage chamber is a connected chamber provided around the inner wall of the liquid nitrogen tank and at the bottom of the inner wall of the liquid nitrogen tank.
  • the upper end surface of the annular hollow chamber or the communicating chamber is provided with an exhaust hole that communicates with the inside of the liquid nitrogen tank, and the inner wall side is provided with a nitrogen through hole.
  • the storage equipment is a basket-type eccentric mouth tank, equipped with an eccentric tank plug and a turntable on which the basket is placed;
  • the eccentric tank plug is equipped with a central rotating shaft at a position coinciding with the axial center axis of the tank, and the turntable It is rotatably arranged on the central rotating shaft, and a liquid storage cylinder connected to the inside of the tank is provided below the central rotating shaft.
  • the tank body of the basket-type partial-mouth tank is a double-layer vacuum tank including an outer cylinder and an inner cylinder, and the annular hollow chamber or the communicating chamber are located on the wall of the inner cylinder.
  • a liquid adding pipe is provided inside the liquid storage cylinder and extends upward through the bottom of the liquid nitrogen tank and the bottom of the liquid storage cylinder.
  • the lower end of the liquid adding pipe is connected to the tank.
  • An external liquid adding device is connected.
  • the upper end of the liquid adding pipe is provided with an elbow, and the bending direction of the elbow is toward the bottom of the liquid nitrogen tank.
  • a guide tube is installed on the outer wall of the liquid adding pipe.
  • the upper end of the guide pipe is close to the elbow at the upper end of the liquid adding pipe. Its height is close to the height of the liquid adding pipe. The lower end is close to the liquid nitrogen. Internal communication of the tank.
  • the basket is provided with multiple layers of aluminum plates, and each layer of aluminum plates is provided with several storage slots for freezing boxes.
  • the storage device is a honeycomb-type open tank, including a honeycomb-type storage structure for placing cryogenic tubes, and the honeycomb-type storage structure is installed on the inner wall of the tank through a flange plate.
  • the honeycomb storage structure includes a storage plate and an aluminum tube.
  • the storage plate is provided with a plurality of aluminum tube storage holes, and an aluminum tube for placing frozen tubes is installed in each aluminum tube storage hole.
  • memory board Fitted with flange plate.
  • the annular hollow chamber is formed by a lining cylinder, an inner wall of the tank, and a flange plate, and the upper and lower ends of the lining cylinder are fixedly connected to the flange plate and the inner wall of the tank respectively.
  • the tank is a double-layer vacuum tank, which is mainly composed of an outer wall of the tank and an inner wall of the tank, and the lining cylinder is connected to the inner wall of the inner wall of the tank.
  • the storage device of a biological sample cryogenic freezing device of the present invention adds an additional liquid nitrogen storage chamber on the basis of various existing liquid nitrogen tanks.
  • the liquid storage chamber is The cavity is formed on the inner wall of the liquid nitrogen tank.
  • the inner wall here includes both the side wall and the bottom. This fills the extra space between the cryopreservation tube storage device and the inner wall of the tank, and provides an additional space in the form of an independent chamber.
  • a certain amount of liquid nitrogen can be retained more stably through the liquid storage chamber. In particular, it can increase the concentration of liquid nitrogen in the upper space of the tank and enhance the It keeps the temperature inside the liquid nitrogen tank in a deep-low temperature environment, reduces the power requirements for filling liquid nitrogen, and is more energy-saving.
  • Figure 1 is a structural cross-sectional view of the first embodiment of the present invention.
  • Figure 2 is a structural cross-sectional view of the first embodiment of the present invention in an applied state.
  • Figure 3 is an enlarged view of the structure at point A in Figure 2.
  • Figure 4 is a structural cross-sectional view of the second embodiment of the present invention.
  • Figure 5 is a structural cross-sectional view of the second embodiment of the present invention in an applied state.
  • Figure 6 is a structural cross-sectional view of the third embodiment of the present invention.
  • Figure 7 is a structural cross-sectional view of the third embodiment of the present invention in an applied state.
  • tank outer wall 312, tank inner wall, 32, annular hollow chamber, 321, flange plate, 322, lining cylinder, 371, storage plate, 372, aluminum tube.
  • this embodiment provides a storage device for a biological sample cryogenic freezing device, including a liquid nitrogen tank.
  • the liquid nitrogen tank is provided with a liquid nitrogen storage chamber.
  • the liquid storage chamber Formed on the inner wall of the liquid nitrogen tank.
  • This embodiment is a specific application in which the storage equipment is a basket-type offset tank (that is, the liquid nitrogen tank is a basket-type offset tank) and the freezing device is a freezing box.
  • the liquid storage chamber is an annular hollow chamber 12 provided on the inner wall of the liquid nitrogen tank. More specifically, in the way of liquid nitrogen in and out, it can be in the ring
  • the upper end surface of the hollow chamber 12 is provided with an exhaust hole that communicates with the inside of the liquid nitrogen tank. Nitrogen will be discharged from the upper exhaust hole into the tank.
  • the inner wall of the annular hollow chamber 12 is provided with a vent hole. The nitrogen hole is used to input the liquid nitrogen in the liquid nitrogen tank into the annular hollow chamber 12.
  • the annular hollow chamber 12 forms a ring of hollow cavity structure on the inner wall. The liquid nitrogen in the annular hollow chamber 12 continuously communicates with the liquid nitrogen in the liquid nitrogen tank.
  • a small amount of liquid nitrogen can be used to create a structure covering the liquid nitrogen tank.
  • the low-temperature area within the body height range can more stably maintain a certain amount of liquid nitrogen.
  • the upward supply of nitrogen from the annular hollow chamber 12 can also increase the liquid nitrogen concentration in the upper space of the tank, which is beneficial to the balance of the upper and lower cooling, and overall It has a certain leak-proof and leak-repairing effect on the liquid nitrogen inside the liquid nitrogen tank, which ultimately allows the temperature inside the liquid nitrogen tank to maintain a deep and low-temperature environment more continuously.
  • the tank body of the basket-type partial mouth tank is a double-layer vacuum tank including an outer cylinder 111 and an inner cylinder 112, and the annular hollow chamber 12 is located on the wall of the inner cylinder 112. superior.
  • the design of the double-layer vacuum tank obviously has better thermal insulation effect.
  • the basket-type eccentric jar is provided with an eccentric jar plug 13 and a turntable 161 for placing the basket; the eccentric jar plug 13 is used to install a central rotating shaft 142, and the turntable 161 is rotatable Provided on the central rotating shaft 142, the rotating disk 161 is provided with a plurality of basket storage support holes.
  • the specific style of the basket in this embodiment is to be provided with multiple layers of aluminum trays, and each layer of aluminum trays is provided with a plurality of freezing box storage slots 162 .
  • the liquid storage cylinder 152 is arranged below the central rotating shaft 142, and the liquid storage cylinder 152 is connected with the inside of the tank.
  • the eccentric tank plug 13 is used to open and close the tank mouth, and has a heat preservation function; specifically, the eccentric tank plug 13 can include a heat preservation cover and an extraction cover, and the heat preservation cover is provided on the tank body.
  • the top of the tank covers the axial center axis 4 area of the tank, which is used for the internal insulation of the tank and the maintenance window inside the tank.
  • the extraction cover is set on the top of the tank, located at the axial center of the tank.
  • the periphery of the shaft area and located on the insulation cover are used for sample storage and extraction.
  • This embodiment may also include a driving device 141 located outside the tank for driving the central rotating shaft 142 to drive the turntable 161 to rotate.
  • the driving device 141 is installed on the eccentric tank plug 13 and passes through the eccentric tank plug 13 through the central rotating shaft 142 to the inside of the tank body. More specifically, the driving device 141 may be a servo motor, the axial center axis of the central rotating shaft 142 coincides with the axial central axis of the tank, and the axial center axis of the central rotating shaft 142 and the central rotating shaft fixed cylinder 143 Coaxial connection.
  • the central rotating shaft fixed cylinder 143 has an open lower end and a bell-shaped bottom at the upper end.
  • the inner side of the bottom of the upper end is fixed with a short axis along the axial center axis direction of the central rotating shaft fixed cylinder 143 and the upper end of the liquid storage cylinder 152.
  • Some bearing seats 151 cooperate through bearings so that the central rotating shaft fixed cylinder 143 is sleeved on the outside of the liquid storage cylinder 152.
  • the lower end of the central rotating shaft fixed cylinder 143 is provided with a flange to form a support base 153 for supporting. Hold the bottom of the carrier.
  • the liquid storage cylinder 152 is a straight cylindrical cavity, the lower end is installed on the bottom surface inside the tank, and the upper end is provided with a bearing seat 151; the interior is hollow and used to store the liquid nitrogen.
  • the axial center axis of the liquid cylinder 152 coincides with the axial center axis of the tank.
  • the inside of the liquid storage cylinder 152 is provided with a liquid adding pipe 154 extending upward through the bottom of the liquid nitrogen tank and the bottom of the liquid storage cylinder 152.
  • the lower end of the liquid adding pipe 154 is connected to the tank.
  • An external liquid adding device is connected.
  • the upper end of the liquid adding pipe 154 is provided with an elbow, and the bending direction of the elbow is toward the bottom of the liquid nitrogen tank.
  • a guide tube 155 is installed on the outer wall of the liquid adding pipe 154.
  • the upper end of the guide pipe 155 is close to the elbow at the upper end of the liquid adding pipe 154. Its height is close to the height of the liquid adding pipe 154.
  • the lower end of the guide pipe 155 is close to the elbow of the upper end of the liquid adding pipe 154. Internal communication of nitrogen tank.
  • this embodiment provides a storage device for a biological sample cryogenic freezing device, which includes a liquid nitrogen tank.
  • the liquid nitrogen tank is provided with a liquid nitrogen storage chamber.
  • Liquid storage chamber Formed on the inner wall of the liquid nitrogen tank.
  • the storage equipment is a basket-type offset tank (that is, the liquid nitrogen tank is a basket-type offset tank) and the freezing device is a freezing box.
  • the liquid storage chamber is a communication chamber 22 provided around the inner wall of the liquid nitrogen tank and at the bottom of the inner wall of the liquid nitrogen tank. Furthermore, the upper end surface of the communication chamber 22 is provided with an exhaust hole that communicates with the inside of the liquid nitrogen tank, and the inner wall side is provided with a nitrogen through hole.
  • the tank body of the basket-type partial mouth tank is a double-layer vacuum tank including an outer cylinder 211 and an inner cylinder 212, the communication chambers 22 are all located on the wall of the inner cylinder 212. superior.
  • Embodiment 2 is consistent with Embodiment 1 in terms of working principle and other structures, such as the eccentric tank plug 23, the driving device 241, the central rotating shaft 242, the liquid adding pipe 254, the guide pipe 255, the bearing seat, the liquid storage cylinder 322, and the support.
  • the base, turntable, storage slot, etc. will not be described in detail.
  • the communicating chamber 22 in this embodiment 2 is equivalent to adding a head after opening the bottom of the annular hollow chamber 12 in the above-mentioned embodiment 1, so as to make use of the redundant bottom that has not been used before.
  • the space is converted into a liquid nitrogen storage cavity, further increasing the space at the bottom of the liquid storage and optimizing the overall long-lasting cold preservation effect.
  • this embodiment provides a storage device for a biological sample cryogenic freezing device, including a liquid nitrogen tank.
  • the liquid nitrogen tank is provided with a liquid nitrogen storage chamber.
  • the liquid storage chamber Formed on the inner wall of the liquid nitrogen tank.
  • the storage device is a honeycomb open tank (that is, the liquid nitrogen tank is a honeycomb open tank) and the freezing device is a cryopreservation tube.
  • the honeycomb open tank includes a honeycomb storage structure for placing freezing tubes, and the honeycomb storage structure is installed on the inner wall of the tank through a flange plate 321.
  • the honeycomb storage structure includes a storage plate 371 and an aluminum tube 372.
  • the storage plate 371 is provided with There are multiple aluminum tube storage holes, and an aluminum tube 372 for placing cryogenic tubes is installed in each aluminum tube storage hole.
  • the storage plate 371 is cooperatively connected with the flange plate 321.
  • the liquid storage chamber is an annular hollow chamber 32 provided on the inner wall of the liquid nitrogen tank. More specifically, the annular hollow chamber 32 is surrounded by a lining cylinder 322, an inner wall of the tank body, and a flange plate 321. The upper and lower ends of the lining cylinder 322 are respectively connected with the flange plate 321 and the inside of the tank. Wall fixed connection. More preferably, the tank is a double-layer vacuum tank, which is mainly composed of a tank outer wall 311 and a tank inner wall 312. The lining cylinder 322 is connected to the inner wall of the tank inner wall 312.
  • Embodiment 3 is still to add a liquid storage chamber to the original liquid nitrogen tank.
  • the liquid storage chamber here specifically utilizes the space between the flange plate 321 and the inner side of the tank. , this space is designed as a partition (i.e., annular hollow chamber 32) to place liquid nitrogen. It can not only effectively enhance the liquid nitrogen storage capacity, but also realize the cooling of the bottom cold air to the upper part through the liquid storage cavity structure such as the aluminum tube 372 and the inner lining cylinder 322, so as to realize the balance between the upper layer cooling and the lower part, both of which keep the temperature inside the tank at a deep level. Low temperature environment.
  • the flange plate 321 forms a support for the flange plate 321, so that the flange plate 321 can be thinned to reduce the amount of processing, the amount of welding, and the amount of deformation.
  • the supporting capacity is increased, so that the load can be stored in the storage plate 371 above the flange plate 321. The ability of samples is enhanced.
  • a storage device for a cryogenic freezing device for biological samples can use various other types of existing tank structures, and the freezing device can also be an existing one. All structural types, as long as they are equipped with a liquid storage chamber that is the central idea of the present utility model, can solve the technical problems of the present utility model.

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  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Un dispositif de stockage d'un appareil de cryoconservation à basse température d'échantillon biologique comprend un corps de réservoir d'azote liquide. Une cavité de stockage d'azote liquide est formée dans le corps de réservoir d'azote liquide, et la cavité de stockage de liquide est formée sur la paroi interne du corps de réservoir d'azote liquide. Selon le dispositif de stockage, un espace inoccupé du dispositif existant peut être complètement utilisé, une quantité de stockage d'azote liquide est augmentée, et la durabilité à basse température de stockage d'échantillon dans un environnement d'hypothermie profonde est améliorée.
PCT/CN2023/078339 2022-03-11 2023-02-27 Dispositif de stockage d'appareil de cryoconservation à basse température d'échantillon biologique WO2023169239A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202220532613.XU CN217321606U (zh) 2022-03-11 2022-03-11 一种生物样本低温冻存装置储存设备
CN202220532613.X 2022-03-11

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WO2023169239A1 true WO2023169239A1 (fr) 2023-09-14

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN217321606U (zh) * 2022-03-11 2022-08-30 上海原能细胞生物低温设备有限公司 一种生物样本低温冻存装置储存设备

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JP2020125139A (ja) * 2019-02-05 2020-08-20 タイガー魔法瓶株式会社 保温容器
CN212481784U (zh) * 2020-06-08 2021-02-05 武汉大学中南医院 一种自动存储液氮罐
CN214147396U (zh) * 2020-12-07 2021-09-07 上海原能细胞生物低温设备有限公司 一种低能耗液氮罐
CN214877250U (zh) * 2021-05-11 2021-11-26 上海原能细胞生物低温设备有限公司 一种样本储存罐
CN215862857U (zh) * 2020-10-09 2022-02-18 南阳杜尔气体装备有限公司 一种改进型液氮储存罐
CN217321606U (zh) * 2022-03-11 2022-08-30 上海原能细胞生物低温设备有限公司 一种生物样本低温冻存装置储存设备

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US4481779A (en) * 1983-06-22 1984-11-13 Union Carbide Corporation Cryogenic storage container
CN203776007U (zh) * 2014-04-02 2014-08-20 新乡学院 一种用于长期保存细胞及生物材料的液氮罐
CN105857937A (zh) * 2016-04-28 2016-08-17 上海原能健康管理有限公司 液氮罐、冻存管存取装置以及液氮罐存取系统
CN206813690U (zh) * 2017-04-20 2017-12-29 山东省滨州畜牧兽医研究院 一种应用于蛋鸡检测的菌种储存罐
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JP2020125139A (ja) * 2019-02-05 2020-08-20 タイガー魔法瓶株式会社 保温容器
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CN217321606U (zh) * 2022-03-11 2022-08-30 上海原能细胞生物低温设备有限公司 一种生物样本低温冻存装置储存设备

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