WO2023161672A1 - Methods and devices to monitor evaporation in culture plates used for air sampling - Google Patents

Methods and devices to monitor evaporation in culture plates used for air sampling Download PDF

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Publication number
WO2023161672A1
WO2023161672A1 PCT/IB2022/051560 IB2022051560W WO2023161672A1 WO 2023161672 A1 WO2023161672 A1 WO 2023161672A1 IB 2022051560 W IB2022051560 W IB 2022051560W WO 2023161672 A1 WO2023161672 A1 WO 2023161672A1
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WO
WIPO (PCT)
Prior art keywords
sampling
culture medium
culture
devices
air
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Application number
PCT/IB2022/051560
Other languages
French (fr)
Inventor
Ahmad AAMER
Mohamed AAMER
Original Assignee
Aamer Ahmad
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aamer Ahmad filed Critical Aamer Ahmad
Priority to PCT/IB2022/051560 priority Critical patent/WO2023161672A1/en
Publication of WO2023161672A1 publication Critical patent/WO2023161672A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/22—Devices for withdrawing samples in the gaseous state
    • G01N1/2202—Devices for withdrawing samples in the gaseous state involving separation of sample components during sampling
    • G01N1/2208—Devices for withdrawing samples in the gaseous state involving separation of sample components during sampling with impactors

Definitions

  • the methods includes:
  • the methods and devices of the current invention provides means for hydrating and decreasing the desiccation and/or dryness occurs in microorganisms collected on the surface of said culture mediums during viable/microbiological culture sampling of environmental air and or gases by reducing water evaporation from the cells of collected microorganisms.
  • the methods and devices of the current invention provides means for using the said culture medium desiccation level in measuring the culture medium thickness and use it along with other factors in determine the actual said collection efficiency of active air sampling process (forced or volumetric air sampling) and accordingly provides the user of actual measured said collection efficiency of every sampling process.
  • the devices of the current invention incorporates one or all of the above mentioned methods in in controlling the sampling duration, desiccation rate and usage/sampling duration of said culture mediums used in:
  • the devices of the current invention are made of materials complaint with the GMP requirements for cleanrooms and controlled environments.
  • the devices of the current invention are integrated with a single use disposable culture medium atriums that includes the culture medium or reusable atriums that includes the culture medium.

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  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Thermal Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention generally provides methods and devices for controlling & decreasing the desiccation rate as well as controlling & increasing the usage/sampling duration of culture mediums used for passive or active sampling and/or monitoring, of microbiological/ biological particles in air/gases in environments where the microbiological quality of air and/or gases is of concern; such as cleanroom environments in variety of industries, for example: pharmaceuticals, bio-pharmaceuticals and electronics manufacturing, e.g. facilities aseptic and/or non-aseptic environments. The invention provides devices that incorporate methods for insulation, cooling, weighing culture medium containers (plates), thus reduces and monitor the desiccation level of culture medium plates and accordingly increasing the usage/sampling duration of single culture medium plate without impacting the viability and survival of the collected microorganisms. The invention provides devices that incorporate methods to continuously monitor the collection efficiency of the active air sampling. The invention provide methods and devices that allow for continuous active air sampling, increasing the usage/sampling duration of single culture medium unit/plate, decreasing the number of culture medium plates per single monitoring location, decrease the probability of false contamination and decrease the risk of introducing contamination to products in areas of monitoring.

Description

Methods and devices for control and monitor the usage/sampling duration of microbiological growth/culture mediums in viable/microbiological environmental sampling of air and/or gases
The microbiological/viable measurement/monitoring of the quality of air/environmental air and or gases.
Microbiological culture/growth mediums are used for monitoring/measuring the quality of environmental air and/or gases by allowing the viable/microbiological particles or carrying particles to come in contact with the culture/growth mediums e.g. TSA medium, Sabouraud medium, etc. Later, the culture/growth mediums are incubated at a specific temperature and for a specific period of time to allow for the growth of collected microorganisms to reach a visible detectable level.
the viable/microbiological particles or carrying particles are collected on the surface of the culture/growth mediums by passive means (called settle plate method) or active means (forced sampling of a specific volume of air and/or gas).
Sampling/ monitoring durations are limited by the capability of the culture/growth mediums to withstand medium desiccation. Culture/growth mediums desiccation would limit its capability of growing all the collected microorganisms and accordingly may result in complete absence or lower number of colonies on culture/growth medium surface that is called false negative results.
The invention represents methods and their application devices for extending the duration of usage/sampling of culture/growth mediums, which is used in environmental monitoring of air and/or gases for measuring the microbiological/viable quality and provides means for a validated continuous calculation of the usage/sampling duration.
The methods includes:
  • Placing the culture/growth mediums container (plates) during the sampling duration in an insulation system that only allow the culture/growth mediums surface to be exposed to the environmental air/gases under sampling.
  • Placing the culture/growth mediums container (plates) during the sampling duration in a cooling system that lowers the temperature of the culture/growth mediums either a continuous cooling system or an intermittent cooling system.
  • Placing the culture/growth mediums container (plates) during the sampling duration in a weighing system that measures the weight of the culture/growth mediums before, during and after the sampling process.
  • A system for using the culture/growth mediums container (plates) weights data for calculation of the efficiency of the active air sampling (forced sampling of a specific volume of air and/or gas) process (so called physical efficiency).
Devices that applies the current invention methods in environmental sampling of the microbiological/viable quality of air/environmental air and or gases including:
  • Device that includes insulation system and/or cooling system and/or weighing system for passive sampling (so called settle plates).
  • Portable active air sampling Device that includes insulation system and/or cooling system and/or weighing system and/or efficiency calculation system for active sampling (so called forced or volumetric sampling).
Remote active air sampling Device using reusable or disposable atriums/heads/plates that includes insulation system and/or cooling system and/or weighing system and/or efficiency calculation system for active sampling (so called forced or volumetric sampling).
Microbiological culture/growth mediums used in environmental monitoring of air and/or gases could only be used for a limited duration and/or limited volume of air to avoid culture/growth mediums desiccation. Culture/growth mediums desiccation would limit their capabilities to recover the collected microorganisms on the culture/growth mediums surface, which would result in lower or complete absence of microorganisms (false negative results).
Culture/growth mediums desiccation would result in:
  • loss of water in medium
  • Change in physical characteristics of the culture medium such as pH and Change in physical characteristics of the culture medium
  • Physical damage in culture medium such as cracks
To avoid desiccations, Microbiological culture/growth mediums could only be used for a limited duration (e.g. 4 hours exposure duration for settle plates) and/or limited volume of air (e.g. 1 cubic meter of air for active air sampling).
To conduct continuous monitoring, Microbiological culture/growth mediums need to be frequently replaced by fresh ones which will results in:
  • High cost of environmental sampling due to using high number of Microbiological culture/growth medium plates.
  • Bigger space and bigger equipment required to handle high number of Microbiological culture/growth medium plates e.g. incubator and canisters.
  • Higher workload required to manage high number of Microbiological culture/growth medium plates sampling, handling, reading and recording or results.
  • Excessive number of medium plates would result in increased probability of false contamination during handing (false positive) and increased risk for introducing contamination to products and or processes arising from the excessive sampling units.
Exact level of Culture/growth mediums desiccation that a plate would reach is unknown, which may results in undetected Culture/growth mediums desiccation level more than the validated accepted level.
Culture/growth mediums desiccation results in decrease of the Culture/growth medium thickness (agar thickness) that changes the distance between nozzles of the active sampling system and the Culture/growth medium surface and accordingly change the overall so called physical efficiency of the active sampling method.
The invention represents a solution that integrates methods with devices to:
  1. Reduce the desiccation level of Culture/growth mediums by reducing the rate of water evaporation form Culture/growth mediums. This is done by placing the Culture/growth mediums in an insulation and/or cooling system that would deprive the Culture/growth mediums from gained temperature from the surrounding atmosphere, which is necessary for water evaporation from the medium exposed surface to environmental air.
  2. Increase the Culture/growth mediums capabilities to recover microorganisms by reducing the desiccation level & the amount of water loss in the mediums and accordingly reduces the physical damage and physical properties changes in the Culture/growth mediums.
  3. Allow for continuous monitoring using a single Culture/growth medium plate by extending the sampling/exposure duration. This is done by extending the duration at which the maximum allowable desiccation level would be reached.
  4. Reduce the cost, workload and bigger space, plates & equipment required in case of using multiple Culture/growth medium plates for short durations.
  5. Reduce the number of units required to conduct the environmental sampling and accordingly decrease the risk of false positive and the risk of introducing contamination to products and or processes.
  6. Allow for accurate determination of desiccation level by that measuring the weight of the culture/growth mediums before, during and after the sampling process and accordingly determine the exact level of medium desiccation and the timing for termination of sampling for a single Culture/growth medium plate.
  7. Measuring the accurate desiccation level would allow for the calculation of the Culture/growth medium thickness reduction and accordingly the calculation of the change the overall so called physical efficiency of the active sampling method.
Decrease the desiccation level of culture mediums used in monitoring microbiological quality of environments, air or gases.
Decrease the desiccation level of microorganisms collected on the surface of culture mediums used in monitoring microbiological quality of environments, air or gases.
Decrease or prevent the physical damage / cracks in culture mediums used in monitoring microbiological quality of environments, air or gases
Allow for extending the usage/sampling duration of culture mediums used in monitoring microbiological quality of environments, air or gases
Allow for decreasing the number of plates of culture mediums required in monitoring microbiological quality of environments, air or gases
Allow for continuous active air sampling of the whole duration of manufacturing shifts.
Allow for actual calculation of the desiccation level of culture mediums used in monitoring microbiological quality of environments, air or gases
Allow for actual calculation of the collection efficiency of active air sampling for monitoring microbiological quality of environments, air or gases
Reduce the cost, labor work, workload, tools and equipment that are necessary for conducting monitoring of microbiological quality of environments, air or gases
Reduce the risk of false positive contamination of culture mediums used in monitoring microbiological quality of environments, air or gases
Reduce the risk introducing contamination to products from extensive number of units of culture mediums used in monitoring microbiological quality of environments, air or gases
Example of device for passive sampling (settle plates)
Example of portable device for active sampling (volumetric sampling)
Example of device for remote active sampling (volumetric sampling)
In an embodiment, for example, the methods and devices of the current invention provides means for controlling the usage/sampling duration and desiccation rate of said microbiological culture mediums that are used for monitoring the biological, microbiological and/or viable particles of air and or gases quality in areas, spaces, rooms, cleanrooms, facilities, lines and/or any other air source where air microbiological quality of concern.
In an embodiment, for example, the methods and devices of the current invention provides means for controlling the desiccation rate of said microbiological culture mediums by placing the culture medium containers (plates) in an insulation system that leaves only the culture medium surface exposed to the environment under monitoring.
In an embodiment, for example, the methods and devices of the current invention provides means for controlling the desiccation rate of said microbiological culture mediums by placing the culture medium containers (plates) in a cooling system that leaves only the culture medium surface exposed to the environment under monitoring.
In an embodiment, for example, the methods and devices of the current invention provides means for cooling and/or insulation the said culture mediums by preventing the medium from gaining temperature from the surrounding environment and/or lowering the said culture medium temperature during the sampling duration. This will decrease the amount of water evaporated from the said culture medium during the sampling and accordingly desiccation/dryness rate of the said culture medium.
In an embodiment, for example, the methods and devices of the current invention provides means for intermittent or continuous cooling and/or insulation the said culture mediums that avoids affecting the viability/survival of said collected microorganisms on the top surface of the said culture medium.
In an embodiment, for example, the methods and devices of the current invention provides means for that avoids affecting the viability/survival of said collected microorganisms on the top surface of the said culture medium by:
  • Allow for selection the cooling temperature of the culture medium based on the survival temperature of the targeted microorganisms
  • Allow for selection the cooling duration of the culture medium based on the validation survival duration of the targeted microorganisms
  • Allow for hydrating microorganisms during the sampling duration and avoid microorganisms desiccation
  • Allowing microorganisms collected on the top surface of culture mediums for gaining heating from environmental air that comes in contact with the top surface of culture mediums
In an embodiment, for example, the methods and devices of the current invention provides means for controlling the usage/sampling duration of said culture mediums by controlling the desiccation rate of said culture mediums.
In an embodiment, for example, the methods and devices of the current invention provides means for decreasing the desiccation and/or dryness of said culture mediums during viable/microbiological culture sampling of environmental air and or gases.
In an embodiment, for example, the methods and devices of the current invention provides means for hydrating and decreasing the desiccation and/or dryness occurs in microorganisms collected on the surface of said culture mediums during viable/microbiological culture sampling of environmental air and or gases by reducing water evaporation from the cells of collected microorganisms.
In an embodiment, for example, the methods and devices of the current invention provides means for avoiding water condensation over culture medium surface leaving the culture medium surface dry at the end of the sampling process by controlling the timing of turning on or switching off the cooling system.
In an embodiment, for example, the methods and devices of the current invention provides means for decreasing the number of units/plates of said culture mediums required for sampling as they result in decreasing the desiccation of the said culture medium plates and accordingly will lead to increase the duration at which the maximum allowable desiccation rate would be reached. Thus, fewer number of said culture medium units/plates are required to cover a specific duration of time.
In an embodiment, for example, the methods and devices of the current invention provides means for decreasing the risk of false contamination of said culture mediums and the risk of introducing contamination to products and or processes arising from the excessive number and handling of culture medium plates.
In an embodiment, for example, the methods and devices of the current invention provides means for decreasing and/or preventing the cracks of said solid culture mediums during viable/microbiological culture sampling of environmental air and or gases which might arise from the desiccation of said culture mediums.
In an embodiment, for example, the methods and devices of the current invention provides means for decreasing and/or preventing the thickness reduction of said solid culture mediums during viable/microbiological culture sampling of environmental air and or gases which might arise from the desiccation of said culture mediums.
In an embodiment, for example, the methods and devices of the current invention provides means for monitoring the level of said culture medium desiccation rate before, during and after the sampling by measuring the culture medium containers weights. Measuring the said culture medium containers weight before, during and after the sampling process would give an accurate measurement of the desiccation level of the said culture medium by knowing how much water had been evaporated from the medium.
In an embodiment, for example, the methods and devices of the current invention provides means for using the said culture medium desiccation level in measuring the culture medium thickness and use it along with other factors in determine the actual said collection efficiency of active air sampling process (forced or volumetric air sampling) and accordingly provides the user of actual measured said collection efficiency of every sampling process.
In an embodiment, for example, the devices of the current invention incorporates one or all of the above mentioned methods in in controlling the sampling duration, desiccation rate and usage/sampling duration of said culture mediums used in:
  • Passive sampling (settle plates)
  • active air sampling (volumetric or forced sampling)
  • remote active air sampling (volumetric or forced sampling)
In an embodiment, for example, the devices of the current invention provide procedure for continuous active air sampling that might be used in accordance with or to replace passive sampling settle plates.
In an embodiment, for example, the devices of the current invention are standalone units or integrated units with other machines/hoods/cabinets/systems/units.
In an embodiment, for example, the devices of the current invention are made of materials complaint with the GMP requirements for cleanrooms and controlled environments.
In an embodiment, for example, the devices of the current invention are made of materials that are compliant with the routine decontamination and/or sterilization methods applied in cleanrooms and controlled environments.
In an embodiment, for example, the devices of the current invention are automatic or manually operated devices.
In an embodiment, for example, the devices of the current invention are of automatic or manual procedures for adding and removing (pop-up) the said culture medium plates/containers from the devices.
In an embodiment, for example, the devices of the current invention are applying the cooling method to said culture medium plates/containers from lateral sidewalls or bottom of the said culture medium plates/containers.
In an embodiment, for example, the devices of the current invention may have an adapter that connects it to the compressed air/gases sources in case of compressed air/gases sampling.
In an embodiment, for example, the devices of the current invention are integrated with a single use disposable culture medium atriums that includes the culture medium or reusable atriums that includes the culture medium.
In an embodiment, for example, the devices of the current invention are only in contact with the outer surface of said culture medium container and do not include a physical contact with said growth culture medium before, during and after sampling process.
Examples
As mentioned in embodiments
Monitoring the microbiological and/or viable air/gases quality in areas, environments, spaces, rooms, cleanrooms, facilities, lines and/or any other air source where air microbiological quality of concern
PTL1:
NPL1:

Claims (25)

  1. A method for controlling the usage/sampling duration and desiccation rate of said microbiological culture mediums, the method includes one and/or all of the following:
    Placing the culture medium containers (plates) in an insulation system that leaves only the culture medium surface exposed to the environment under monitoring and thus preventing the culture medium from gaining temperature from the surrounding environment that leads to increase the water evaporation rate (desiccation) from the culture mediums.
    Placing the culture medium containers (plates) in a cooling system that leaves only the culture medium surface exposed to the environment under monitoring and thus decreasing the culture medium temperature during the sampling duration, which leads control the water evaporation rate (desiccation) from the culture mediums and accordingly control the duration at which the culture medium would reach the maximum allowable desiccation rate. The cooling system allows for selection of cooling temperature and cooling duration.
  2. The method of claim 1 compromising a continuous and/or intermittent cooling system of said culture medium containers (plates) during sampling duration that allows for selection of cooling temperature and cooling duration.
  3. The method of claim 1 compromising a cooling system of said culture medium containers (plates) during sampling duration that avoids water condensation over culture medium surface leaving the culture medium surface dry at the end of the sampling process.
  4. A method for decreasing the number of said culture medium units (plates) used for environmental sampling by extending the usage/sampling duration of a single unit and accordingly decreasing the risk of false contamination of culture medium units (plates) and the risk of introducing contamination to products and or processes arising from the excessive number and handling of culture medium plates.
  5. A method for decreasing the desiccation and/or dryness of said culture mediums during viable/microbiological culture sampling of environmental air and or gases.
  6. A method for decreasing the desiccation and/or dryness occurs in microorganisms collected on the surface of said culture mediums during viable/microbiological culture sampling of environmental air and or gases
  7. A method for decreasing and/or preventing the cracks of said solid culture mediums during viable/microbiological culture sampling of environmental air and or gases.
  8. A method for decreasing and/or preventing the thickness reduction of said solid culture mediums during viable/microbiological culture sampling of environmental air and or gases.
  9. A method for hydrating the surface of said culture mediums and/or microorganisms during viable/microbiological culture sampling of environmental air and or gases.
  10. A method for monitoring the level of said culture medium desiccation rate before, during and after the sampling by measuring the culture medium containers weights.
  11. A method for using the said culture medium desiccation level in measuring the culture medium thickness and use it along with other factors in determine the actual collection efficiency of active air sampling process (forced or volumetric air sampling).
  12. A device that incorporates a single method or all methods in claims from claim 1 to claim11 in controlling the sampling duration, desiccation rate and usage/sampling duration of said culture mediums used in passive sampling (settle plates).
  13. A portable device that incorporates a single method or all methods in claims from claim 1 to claim11 in controlling the sampling duration, desiccation rate and usage/sampling duration of said culture mediums used in active air sampling (volumetric or forced sampling).
  14. A device that incorporates a single method or all methods in claims from claim 1 to claim11 in controlling the sampling duration, desiccation rate and usage/sampling duration of said culture mediums used in remote active air sampling (volumetric or forced sampling).
  15. The devices of claim 12, 13 and 14 are standalone units or integrated units with other machines/hoods/cabinets/systems/units.
  16. The devices of claim 12, 13 and 14 are made of materials complaint with the GMP requirements for cleanrooms and controlled environments.
  17. The devices of claim 12, 13 and 14 are made of materials that are compliant with the routine decontamination and/or sterilization methods applied in cleanrooms and controlled environments.
  18. The devices of claim 12, 13 and 14 are automatic or manually operated devices.
  19. The devices of claim 12, 13 and 14 are of automatic or manual procedures for adding and removing (pop-up) the said culture medium plates/containers from the devices.
  20. The devices of claim 12, 13 and 14 are applying the cooling method to said culture medium plates/containers from lateral sidewalls or bottom of the said culture medium plates/containers.
  21. The devices of claim 12, 13 and 14 are used for monitoring the biological, microbiological and/or viable particles of air and or gases quality in areas, spaces, rooms, cleanrooms, facilities, lines and/or any other air source where air microbiological quality of concern.
  22. The devices of claim 12, 13 and 14 may have an adapter that connects it to the compressed air/gases sources in case of compressed air/gases sampling.
  23. The remote units of device of claim 14 are integrated with a single use disposable culture medium atriums that includes the culture medium or reusable atriums that includes the culture medium.
  24. The devices of claim 12, 13 and 14 are only in contact with the outer surface of said culture medium container and do not include a physical contact with said growth culture medium before, during and after sampling process.
  25. The methods of claims 1,2,3,4,5,6,7,8,9,10 and 11 and devices of claim 12, 13 and 14 provide procedure for continuous active air sampling that might be used in accordance with or to replace passive sampling settle plates.
PCT/IB2022/051560 2022-02-22 2022-02-22 Methods and devices to monitor evaporation in culture plates used for air sampling WO2023161672A1 (en)

Priority Applications (1)

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PCT/IB2022/051560 WO2023161672A1 (en) 2022-02-22 2022-02-22 Methods and devices to monitor evaporation in culture plates used for air sampling

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Application Number Priority Date Filing Date Title
PCT/IB2022/051560 WO2023161672A1 (en) 2022-02-22 2022-02-22 Methods and devices to monitor evaporation in culture plates used for air sampling

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020162773A1 (en) * 2001-05-02 2002-11-07 Kim Sang Soo Impactor with cooled impaction plate and method for classifying and collecting aerosols using the same
US20120255861A1 (en) * 2010-09-27 2012-10-11 Susanne Vera Hering Growth tube microchip electrophoresis system
GB2510501A (en) * 2011-11-28 2014-08-06 Rothamsted Res Ltd Air sampling device
US20180128671A1 (en) * 2014-12-17 2018-05-10 Karlsruher Institut Fuer Technologie Device for measuring superfine particle masses
US20200278275A1 (en) * 2017-09-12 2020-09-03 University Of Northumbria At Newcastle Impactor for aerosol component collection

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020162773A1 (en) * 2001-05-02 2002-11-07 Kim Sang Soo Impactor with cooled impaction plate and method for classifying and collecting aerosols using the same
US20120255861A1 (en) * 2010-09-27 2012-10-11 Susanne Vera Hering Growth tube microchip electrophoresis system
GB2510501A (en) * 2011-11-28 2014-08-06 Rothamsted Res Ltd Air sampling device
US20180128671A1 (en) * 2014-12-17 2018-05-10 Karlsruher Institut Fuer Technologie Device for measuring superfine particle masses
US20200278275A1 (en) * 2017-09-12 2020-09-03 University Of Northumbria At Newcastle Impactor for aerosol component collection

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