WO2023124961A1

WO2023124961A1 - Reaction cartridge and detection apparatus

Info

Publication number: WO2023124961A1
Application number: PCT/CN2022/138607
Authority: WO
Inventors: 安光明; 齐志林; 高翥; 章益
Original assignee: 成都齐碳科技有限公司
Priority date: 2021-12-30
Filing date: 2022-12-13
Publication date: 2023-07-06
Also published as: CN114231399A

Abstract

A reaction cartridge and a detection apparatus, the reaction cartridge comprising: a cartridge body (1), the cartridge body being provided with a drive slot (13) and a plurality of reagent reservoirs (15); a connecting member (2), which comprises a connecting plate (21), wherein the connecting plate (21) covers a first end of the cartridge body (1), a first sample loading hole (212) and a plurality of ventilation holes (213) are provided on the connecting plate (21), the first sample loading hole (212) is in communication with one reagent reservoir (15), and the plurality of ventilation holes (213) are in communication with the plurality of reagent reservoirs (15) respectively; a covering member (3), which is arranged on the connecting member (2), the covering member (3) being provided with a second sample loading hole (32), and the second sample loading hole (32) being used to communicate with the first sample loading hole (212) during sample loading; a balance air slot (4) provided on one of the connecting member (2) and the covering member (3), the balance air slot (4) being selectively in communication with or not in communication with the plurality of ventilation holes (213); an adapter (7), which is slidably arranged on a second end of the cartridge body (1), the adapter (7) connecting the drive slot (13) to different reagent reservoirs (15) in a time-sharing manner; and a piston member (10), which is sealably and slidably mounted in the drive slot (13). The detection apparatus comprises the above reaction cartridge.

Description

Reaction cartridge and detection device

Cross References to Related Applications

This application claims priority to the Chinese patent application 202111665856.7 filed on December 30, 2021, entitled "Reaction Cartridge and Detection Device", the entire content of which is incorporated herein by reference.

technical field

The application belongs to the technical field of biological detection, and in particular relates to a reaction cartridge and a detection device.

Background technique

Nucleic acid detection is widely used in various fields of biomedicine, especially in the detection of infectious disease pathogens, the result of nucleic acid detection is an important criterion for determining whether a diagnosis is made or not. The traditional nucleic acid detection process is cumbersome, including steps such as nucleic acid extraction, PCR amplification, and result detection. In the above steps, different detection reagents need to be manually added to the reaction device in sequence, which poses challenges for the detection operators and the experimental environment. Higher requirements, improper manual operation is prone to experimental errors, and the detection process is carried out in an open environment, which is easy to pollute the environment.

Contents of the invention

The embodiments of the present application provide a reaction cartridge and a detection device, which can reduce experimental errors and avoid environmental pollution during the detection process.

On the one hand, the embodiment of the present application provides a reaction cartridge, which includes:

The main body of the cartridge, the main body of the cartridge is provided with a driving slot and a plurality of reagent slots, the bottom of the driving slot is provided with a first through hole, and the bottom of the plurality of reagent slots is respectively provided with a plurality of second through holes;

The connecting piece includes a connection plate, which is arranged on the first end of the cartridge body and covers the reagent tank. The connection plate is provided with a first sample injection hole and a plurality of ventilation holes, and the first sample injection hole communicates with a reagent tank , a plurality of air holes communicated with a plurality of reagent tanks respectively;

The cover part is arranged on the connecting part, and the cover part is provided with a second sample injection hole, and the second sample injection hole is used to communicate with the first sample injection hole when adding a sample;

A balance air groove is formed in one of the connecting piece and the cover piece, and the balance air groove can selectively communicate with or not communicate with a plurality of ventilation holes;

The adapter is slidably arranged on the second end of the main body of the cartridge. An adapter groove is provided on the adapter. One end of the adapter groove is connected to the first through hole, and the other end of the adapter groove can slide with the adapter communicating with a second through hole in the plurality of second through holes;

The piston part passes through the cover part and the connecting part and is sealed and slidably installed in the driving groove.

In some embodiments, a first connection hole is opened on the connection plate, a second connection hole is opened on the cover member, the first connection hole, the second connection hole and the driving groove are connected in sequence, and the piston part passes through the first connection hole and the second connection hole and slide installed in the drive groove.

In some embodiments, the main body of the cartridge is cylindrical, the driving slot is arranged at the center of the main body of the cartridge, and a plurality of reagent tanks are arranged around the driving slot in a ring shape.

In some embodiments, one end of the connection plate is sealed and fixed to the main body of the cartridge, and the other end of the connection plate is slidably connected to the cover. The cover is in the shape of a disk concentric with the main body of the cartridge. Rotating and sliding relative to the connection plate, the second sample injection hole can selectively communicate with or not communicate with the first sample injection hole with the rotation of the cover member.

In some embodiments, the balance air groove is disposed on the cover part and formed on a side surface of the cover part and the connecting part, and the balance air groove is recessed toward a side away from the connecting part.

In some embodiments, the cover can be switched between the closed state and the open state by rotation, and the balance air groove can communicate with or not communicate with the vent hole with the rotation of the cover;

In the open state, the second sample injection hole is connected to the first sample injection hole, and the balance gas groove is not connected to the vent hole;

In the closed state, the second sample injection hole is not connected to the first sample injection hole, and the balance gas groove is connected to the vent hole.

In some embodiments, the covering part is provided with a first actuating structure, and the first actuating structure is used to drive the covering part to rotate.

In some embodiments, the reaction cartridge further includes a first fixing part, the first fixing part is fixed on the first end of the cartridge main body, and the connecting part and the cover part are sandwiched between the cartridge main body and the first fixing part.

In some embodiments, the connecting piece further includes a connecting ring, the connecting ring is connected to the periphery of the connecting plate, and the two ends of the connecting ring are respectively sealed and fixed to the connecting plate and the cover. The space surrounded by the connecting ring is formed, and a sealing piece is installed in the second sample injection hole.

In some embodiments, the reaction cartridge further includes an actuation assembly, the actuation assembly includes an actuation piece and a cover plate, the cover plate is arranged in the balance gas tank and covers all the ventilation holes, and one end of the actuation piece is connected to the cover. The plate is connected, and the other end of the actuator runs through the connection plate and the cartridge body to extend out of the cartridge body to operate the actuator, and the actuator can move back and forth toward the cover to close the cover or When the vent hole is opened, the balance air groove is not connected to the vent hole when the cover plate part covers the vent hole, and the balance air groove is connected to the vent hole when the vent hole is opened by the cover plate part.

In some embodiments, a second actuating structure is provided at an end of the adapter piece away from the main body of the cartridge, and the second actuating structure is used to drive the adapter piece to rotate.

In some embodiments, the reaction cartridge further includes a second fixing part, the second fixing part is fixed on the second end of the cartridge main body, and the adapter part is rotatably installed between the cartridge main body and the second fixing part.

In some embodiments, a rotary assembly is disposed between the adapter and the second fixing member.

In another aspect, the present application provides a detection device, which includes the reaction cartridge as described in any of the above solutions.

The beneficial effect of this application:

The cartridge body of the reaction cartridge provided in the embodiment of the present application is provided with a driving groove and a plurality of reagent grooves, and various reagents can be pre-embedded in the plurality of reagent grooves, and the driving grooves can be connected with different reagents through the rotation of the adapter. The reagent tanks are connected. When performing nucleic acid detection, only the sample needs to be added to a specific reagent tank, and then the reaction reagents in the previous reagent tank can be injected into the next reagent tank through the drive tank in sequence according to the operation sequence, so as to realize automatic detection. Nucleic acid extraction, PCR amplification, and result detection operations are performed without manual intervention throughout the detection process, eliminating experimental errors caused by manual operations. At the same time, the reaction cartridge is sealed to avoid environmental pollution.

Description of drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following will briefly introduce the accompanying drawings that need to be used in the embodiments of the present application. Additional figures can be derived from these figures.

Figure 1 is a schematic structural view of a reaction cartridge provided in some embodiments of the present application;

Fig. 2 is a schematic diagram of the exploded structure of the reaction cartridge provided by some embodiments of the present application;

Fig. 3 is a right view of the cartridge body of the reaction cartridge provided by some embodiments of the present application;

Figure 4 is a schematic structural view of the connectors of the reaction cartridge provided in some embodiments of the present application;

Fig. 5 is a schematic diagram of the first structure of the lid of the reaction cartridge provided by some embodiments of the present application;

Fig. 6 is a structural schematic diagram II of the lid of the reaction cartridge provided in some embodiments of the present application;

Fig. 7 is a schematic structural view of the first fixing part of the reaction cartridge provided by some embodiments of the present application;

Fig. 8 is a schematic structural view of the adapter of the reaction cartridge provided by some embodiments of the present application;

Fig. 9 is a schematic structural view of the second fixing part of the reaction cartridge provided by some embodiments of the present application;

Fig. 10 is a schematic structural view of the rotary assembly of the reaction cartridge provided by some embodiments of the present application;

Fig. 11 is a schematic structural view of the piston part of the reaction cartridge provided by some embodiments of the present application;

Figure 12 is a schematic diagram of the exploded structure of the reaction cartridge provided by other embodiments of the present application;

Figure 13 is a schematic structural view of the connectors of the reaction cartridge provided by other embodiments of the present application;

Fig. 14 is a schematic diagram of the first structure of the lid of the reaction cartridge provided by other embodiments of the present application;

Fig. 15 is a second structural schematic diagram of the lid of the reaction cartridge provided by other embodiments of the present application;

Fig. 16 is a schematic structural view of the actuating assembly of the reaction cartridge provided by other embodiments of the present application.

The diagram is marked as follows:

1. Cartridge main body; 11. Main body part; 12. Connecting part; 13. Drive slot; 14. First through hole; 15. Reagent tank; 16. Second through hole; 2. Connector; 21. Connection plate; 211, the first connection hole; 212, the first sample injection hole; 213, the ventilation hole; 22, the connection ring; 23, the connection hole wall; 24, the sample injection hole wall; 3, the cover piece; 31, the second connection hole ; 32, the second sample injection hole; 33, the first actuating structure; 34, the limit part; 35, the ball groove; 36, the ball; 37, the sealing piece; 4, the balance air groove; 5, the first fixing piece; 51. Ring slide rail; 52. Limiting wall; 53. Limiting groove; 54. Operating hole; 6. Actuating assembly; 61. Actuating part; 711, transfer groove; 72, transfer connection part; 721, second actuating structure; 8, second fixing member; 81, bottom wall; 82, side wall; 83, accommodation groove; 84 , the third through hole; 9, the slewing assembly; 91, the slewing bearing; 92, the gasket; 10, the piston part; 101, the piston part; 102, the piston rod part.

Detailed ways

The characteristics and exemplary embodiments of various aspects of the application will be described in detail below. In order to make the purpose, technical solution and advantages of the application clearer, the application will be further described in detail below in conjunction with the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described here are only intended to explain the present application rather than limit the present application. It will be apparent to one skilled in the art that the present application may be practiced without some of these specific details. The following description of the embodiments is only to provide a better understanding of the present application by showing examples of the present application.

It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply that there is a relationship between these entities or operations. There is no such actual relationship or order between them. Furthermore, the term "comprises", "comprises" or any other variation thereof is intended to cover a non-exclusive inclusion such that a process, method, article, or apparatus comprising a set of elements includes not only those elements, but also includes elements not expressly listed. other elements of or also include elements inherent in such a process, method, article, or device. Without further limitations, an element defined by the statement "comprising..." does not exclude the presence of additional same elements in the process, method, article or device comprising said element.

The orientation terms in the description of the application are only for the convenience of describing the application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be construed as limiting the scope of the application. limit.

It should be noted that, in the case of no conflict, the embodiments in the present application and the features in the embodiments can be combined with each other. The embodiments will be described in detail below in conjunction with the accompanying drawings.

In one aspect, the present application provides a reaction cartridge, which can be used in nucleic acid detection and other biological detection fields. As shown in Figures 1 to 3, the reaction cartridge provided by the embodiment of the present application includes: a cartridge body 1, a connector 2, a cover 3, a balance gas tank 4, an adapter 7 and a piston 10, the cartridge The main body 1 is provided with a driving slot 13 and a plurality of reagent slots 15 , the bottom of the driving slot 13 is provided with a first through hole 14 , and the bottom of the reagent slot 15 is provided with a second through hole 16 .

The connector 2 includes a connection plate 21, which is arranged on the first end of the cartridge body 1 and covers the reagent tank 15. The connection plate 21 is provided with a first sample injection hole 212 and a plurality of ventilation holes 213. The sample hole 212 communicates with one reagent tank 15 , and the plurality of ventilation holes 213 communicates with a plurality of reagent tanks 15 respectively. The cover part 3 is disposed on the connecting part 2, and the cover part 3 is provided with a second sample injection hole 32, which is used to communicate with the first sample injection hole 212 when adding a sample. The balance air groove 4 is formed in one of the connection part 2 and the cover part 3 , and the balance air groove 4 can selectively communicate with or not communicate with the plurality of vent holes 213 . The adapter piece 7 is slidably arranged on the second end of the cartridge main body 1, and the adapter piece 7 is provided with an adapter groove 711, one end of the adapter groove 711 communicates with the first through hole 14, and the other end of the adapter groove 711 can be With the sliding of the adapter piece 7 , it communicates with one second through hole 16 of the plurality of second through holes 16 . The piston part 10 passes through the cover part 3 and the connecting part 2 and is sealed and slidably installed in the driving groove 13 .

The cartridge main body 1 of the reaction cartridge provided in the embodiment of the present application is provided with a driving groove 13 and a plurality of reagent grooves 15, and various reagents can be pre-embedded in the plurality of reagent grooves 15, and the driving can be driven by the rotation of the adapter 7. The tanks 13 communicate with different reagent tanks 15, respectively. When performing nucleic acid detection, only the sample is added to the initial reagent tank 15, and then the reaction reagents in the previous reagent tank 15 can be injected into the next reagent tank 15 through the drive tank 13 in sequence according to the operation sequence, so as to realize Nucleic acid extraction, PCR amplification, and result detection are automatically performed without manual intervention in the whole process, eliminating experimental errors caused by manual operations. At the same time, the reaction cartridge is sealed to avoid environmental pollution.

As shown in FIG. 2 and FIG. 3 , in some embodiments, the cartridge body 1 is cylindrical, the drive slot 13 is disposed at the center of the cartridge body 1 , and a plurality of reagent tanks 15 are arranged around the drive slot 13 in a ring shape. In some embodiments, the cartridge body 1 has a stepped cylindrical shape, which includes a cylindrical main body portion 11 and a connecting portion 12 , and the connecting portion 12 has a larger radius than the main body portion 11 . The drive tank 13 and the reagent tank 15 pass through the connection part 12 and protrude into the main body part 11 . The connecting portion 12 is disposed at the first end of the main body 11 and protrudes outward from the outer peripheral surface of the main body 11 . The connecting portion 12 is used for connecting an external fixing component.

In some embodiments, both the drive groove 13 and the reagent groove 15 are open toward the first end of the cartridge body 1, the first through hole 14 at the bottom of the drive groove 13 is connected to the end surface of the second end of the cartridge body 1, and the reagent groove 15 The second through hole 16 at the bottom communicates with the end surface of the second end of the cartridge main body 1 . The projected cross-sectional area of the first through hole 14 is smaller than the projected cross-sectional area of the driving groove 13 , and the projected cross-sectional area of the second through hole 16 is smaller than the projected cross-sectional area of the reagent tank 15 . In some embodiments, both the first through hole 14 and the second through hole 16 are circular holes.

In some embodiments, the driving groove 13 is a circular groove extending longitudinally, the reagent groove 15 is a fan-shaped groove extending longitudinally, and the reagent groove 15 is fan-shaped along a direction away from the driving groove 13 . A plurality of reagent tanks 15 are arranged at equal intervals in a ring around the drive tank 13, and the number of reagent tanks 15 can be determined according to the detection process, which is not limited in the embodiment of the present application. Of course, in other embodiments, the shape of the driving groove 13 can also be triangular, rectangular or other shapes, and the shape of the reagent groove 15 can also be polygonal or other suitable shapes, as long as a plurality of reagent grooves 15 surround the driving groove in a ring shape. 13 equal intervals can be set.

The structure of the connecting piece 2 is shown in FIG. 4 . In some embodiments, the connecting piece 2 includes a connecting pad 21 . A first connection hole 211 is provided on the connection plate 21, a second connection hole 31 is provided on the cover member 3, the first connection hole 211, the second connection hole 31 and the driving groove 13 are connected in sequence, and the piston member 10 passes through the first connection hole 31. The connecting hole 211 and the second connecting hole 31 are slidably installed in the driving groove 13 .

The connection plate 21 is used to cover a plurality of reagent tanks 15 and connect the cover member 3 at the same time. In some embodiments, the connection plate 21 is disc-shaped, and the connection plate 21 is concentric with the cartridge body 1 , one end of the connection plate 21 is sealed and fixed with the cartridge body 1 , and the other end of the connection plate 21 slides with the cover 3 connect. The first connecting hole 211 on the connecting plate 21 is disposed at the center of the connecting plate 21 and concentric with the driving groove 13 . The first sample injection hole 212 communicates directly with one of the reagent tanks 15, and the projected area of the first sample injection hole 212 on the cartridge main body 1 is within the surrounding range of the reagent tank 15 that it communicates with. . The ventilation holes 213 communicate with the plurality of reagent wells 15 on the cartridge body 1 in one-to-one correspondence, and the projected area of each ventilation hole 213 on the cartridge body 1 is located within the surrounding area of the reagent wells 15 it communicates with.

In some embodiments, the connecting pad 21 is sealed and fixed on the end surface of the first end of the cartridge main body 1 by means of glue, ultrasonic welding or laser welding.

The cover part 3 is used for adding samples and covering the connection part 2 and the cartridge main body 1 during the reaction process. As shown in Figures 5 and 6, in some embodiments, the cover part 3 is slidably connected to the connection plate 21, the cover part 3 is in the shape of a disc concentric with the cartridge main body 1 and the connection part 2, and the cover part 3 It can rotate around its axis and slide relative to the end surface of the connection plate 21 , and the second sample injection hole 32 can selectively communicate with or not communicate with the first sample injection hole 212 with the rotation of the cover member 3 .

The second connecting hole 31 is disposed at the center of the cover member 3 , and the cross-sectional shapes of the first connecting hole 211 , the second connecting hole 31 and the driving groove 13 are the same. In some embodiments, the first connecting hole 211 , the second connecting hole 31 and the driving groove 13 are concentric and all are circular holes.

The cover member 3 has a closed state and an open state. In the covered state, the second sample injection hole 32 is facing the unopened area on the connection plate 21, and the cover member 3 seals the first sample injection hole 212. When the cover member 3 is changed from the cover state to the open state, the cover member 3 is rotated around its axis and slides relative to the connecting member 2 until the second sample injection hole 32 is facing the first sample injection hole 212. The sample loading hole 32 and the first sample loading hole 212 are put into the initial reagent tank 15 , that is, the sample loading operation is completed, and the cover member 3 is turned back to the cover state.

In some embodiments, a sealing layer is provided between the covering part 3 and the connecting part 2, and the sealing layer may include sealing silicone grease, high temperature lubricating oil or other sealing materials. The sealing layer is used to improve the airtightness between the cover part 3 and the connecting part 2, and prevent the reagent tank 15 from communicating with the outside during the test.

As shown in FIG. 5 , in some embodiments, the balance air groove 4 is arranged on the cover part 3 and formed on the side surface of the cover part 3 and the connecting part 2 , and the balance air groove 4 faces away from the connecting part 2 One side is recessed, and the area of the balance gas groove 4 and the second sample injection hole 32 does not overlap. The balance air groove 4 includes a communication groove 41 in the middle and a plurality of extension grooves 42 extending outward from the communication groove 41. The balance air groove 4 can selectively communicate with a plurality of ventilation holes 213 as the cover 3 rotates. or disconnected.

When the cover member 3 is in the cover state, the plurality of extension grooves 42 communicate with the plurality of ventilation holes 213 in one-to-one correspondence. When the cover member 3 is in an open state, the plurality of extending grooves 42 are not in communication with the plurality of ventilation holes 213 . Balance gas groove 4 can make the air pressure in a plurality of reagent grooves 15 keep balance when being communicated with a plurality of ventilation holes 213, prevents reaction reagent from occurring negative pressure in reagent groove 15 when transferring between driving groove 13 and reagent groove 15, guarantees Detection effect.

As shown in FIG. 6 , in some embodiments, a first actuating structure 33 is provided on the side of the cover 3 away from the cartridge body 1 , and the first actuating structure 33 is used to drive the cover 3 to rotate. The first actuating structure 33 may be a rotating handle protruding from the side surface of the cover 3 facing away from the cartridge main body 1 , and it is detected that the operator rotates the cover 3 by turning the rotating handle. In some other embodiments, the first actuating structure 33 may also be a claw structure provided on the outer peripheral surface of the cover 3 , and the detection operator rotates the cover 3 by turning the claw structure.

As shown in Figure 7, in some embodiments, the reaction cartridge further includes a first fixing member 5, the first fixing member 5 is fixed on the first end of the cartridge main body 1, and the connecting member 2 and the cover member 3 are clamped on the Between the cartridge main body 1 and the first fixing member 5 .

In some embodiments, the first fixing member 5 is fixed on the connecting portion 12 of the cartridge main body 1, and the first fixing member 5 and the connecting portion 12 of the cartridge main body 1 are fixed on the Together. In the embodiment shown in FIG. 2 , the first fixing member 5 is fixed together with the cartridge main body 1 through bolt connection. The cover part 3 is rotatably installed between the connecting part 2 and the first fixing part 5 , and the two side surfaces of the covering part 3 are respectively slidably connected with the connecting part 2 and the first fixing part 5 . The first fixing member 5 shown in FIGS. 1 and 2 is connected to the connection portion 12 of the cartridge main body 1 by three bolts, and those skilled in the art can determine the number of bolts as required. In addition, besides the bolt connection, the first fixing member 5 can also be connected to the connection portion 12 of the cartridge main body 1 by buckling, gluing or ultrasonic welding.

As shown in Fig. 1, Fig. 6 and Fig. 7, in some embodiments, a plurality of ball grooves 35 arranged in a ring are arranged on the side surface of the cover part 3 facing the first fixing part 5, and in the ball grooves 35 Ball 36 is installed in it. On the side surface of the first fixing part 5 facing the cover part 3, an annular slide rail 51 is arranged, and the annular slide rail 51 is arranged opposite to a plurality of ball grooves 35, and the balls 36 are sandwiched between the ball grooves 35 and the annular slide rail 51. Inside and can roll along the annular slide rail 51. The smoothness of the sliding of the covering part 3 relative to the first fixing part 5 can be increased by arranging structures such as the ball 36 and the annular slide rail 51 , and the convenience of operation can be improved.

In some embodiments, one of the covering part 3 and the first fixing part 5 is provided with a limiting part 34, and the other is provided with an arc-shaped limiting groove 53, and the limiting part 34 is slidably installed in the limiting groove 53. Inside, the rotation angle of the cover member 3 around its axis is equal to the radian of the limiting slot 53 . The radian of the limiting groove 53 is the angle of the central angle formed by the two ends of the limiting groove 53 and the axis of the cartridge main body 1 . In the embodiment shown in FIG. 6 , the limiting portion 34 is a platform-shaped structure disposed on the cover member 3 and protruding toward the first fixing member 5 . In the embodiment shown in FIG. 7 , a limiting wall 52 protrudes from the side surface of the first fixing member 5 facing the cover member 3 , and the limiting wall 52 surrounds and forms a limiting groove 53 , and the limiting portion 34 It is embedded in the limiting groove 53 and can slide along the limiting groove 53 .

In some embodiments, an operation hole 54 is provided in the middle of the first fixing part 5 , and the second connection hole 31 and the rotating handle on the cover part 3 are located in the surrounding area of the operation hole 54 . The operation hole 54 is convenient for detecting that the operator performs a pulling operation on the piston member 10 and a screwing operation on the cover member 3 by rotating the handle.

The structure of the adapter 7 is shown in FIG. 8 , and the adapter 7 is used to communicate different reagent tanks 15 with the driving tank 13 . The adapter 7 includes a connected adapter body portion 71 and an adapter connection portion 72, both of which are cylindrical in shape, and the radius of the adapter body portion 71 is greater than the radius of the adapter connection portion 72 . The transfer body portion 71 abuts against the cartridge body 1 , and the transfer groove 711 is disposed on a side surface of the transfer body portion 71 facing the cartridge body 1 . A second actuating structure 721 is provided on the side of the adapter connecting portion 72 facing away from the adapter main body 71 , and the second actuating structure 721 is used to drive the adapter 7 to rotate. In the embodiment shown in FIG. 8 , the second actuating structure 721 is a straight groove, and the detection operator applies torque to the second actuating structure 721 to drive the adapter 7 to rotate.

As shown in Figure 9, in some embodiments, the reaction cartridge further includes a second fixing member 8, the second fixing member 8 is fixed on the second end of the cartridge main body 1, and the adapter 7 is rotatably mounted on the cartridge main body 1 and the second fixing member 8. The second fixing member 8 is fixed together with the main body 1 of the cartridge through screw connection, bolt connection or card connection.

The second fixing member 8 includes a bottom wall portion 81 and a side wall portion 82 , the bottom wall portion 81 and the side wall portion 82 surround and form a receiving groove 83 , and the transfer main body portion 71 is accommodated in the receiving groove 83 . In one embodiment, one end of the adapter main body 71 abuts against the inner surface of the bottom wall 81 , and the other end of the adapter main body 71 abuts against the end surface of the second end of the cartridge main body 1 . A third through hole 84 is opened on the bottom wall portion 81 , and the transfer connecting portion 72 passes through the third through hole 84 and protrudes out of the second fixing member 8 .

As shown in FIGS. 2 and 10 , in some embodiments, a rotary assembly 9 is provided between the adapter piece 7 and the second fixing piece 8 . The rotary assembly 9 is arranged in the accommodation groove 83. The rotary assembly 9 includes a rotary bearing 91 and a gasket 92. The inner ring of the rotary bearing 91 is in interference fit with the outer surface of the transfer connection part 72. The outer ring of the rotary bearing 91 is in contact with the side wall. The inside surface of portion 82 is an interference fit. The washer 92 is disposed between the slewing bearing 91 and the adapter main body 71 , and the washer 92 is an elastic washer for pushing the adapter 7 to the second end of the cartridge main body 1 .

The piston member 10 is sealed and slidably installed in the driving groove 13 , and is used to adjust the volume of the driving groove 13 to realize the extraction and injection of the reagent in a certain reagent groove 15 . The structure of the piston part 10 is shown in Figures 2 and 11. In some embodiments, the piston part 10 includes a piston part 101 and a piston rod part 102. The axis slides axially, and the piston rod portion 102 protrudes out of the first fixing member 5 through the first connection hole 211 , the second connection hole 31 and the operation hole 54 , and the piston rod portion 102 is used to drive the piston portion 101 to slide. In the initial state of the experiment, the piston portion 101 abuts against the groove bottom surface of the driving groove 13 .

When it is necessary to extract the reagent in a reagent tank 15 into the drive tank 13, turn the adapter 7 so that the two ends of the transfer tank 711 communicate with the drive tank 13 and the reagent tank 15 respectively, facing away from the adapter 7 At this time, the volume of the drive tank 13 increases, the air pressure in the drive tank 13 decreases, and the reagent is drawn from the reagent tank 15 into the drive tank 13 via the transfer tank 711 . When it is necessary to inject the reagent in the drive tank 13 into another reagent tank 15, the adapter 7 is rotated so that the two ends of the transfer tank 711 communicate with the drive tank 13 and the other reagent tank 15 respectively, and move towards the adapter close to the other reagent tank 15. Slide the piston part 10 in the direction of the part 7, at this time the volume of the drive tank 13 decreases, the air pressure in the drive tank 13 increases, and the reagent is pressed into another reagent tank 15 from the drive tank 13 via the transfer tank 711.

In some embodiments, a lubricating layer is provided between the side of the piston part 101 and the inner surface of the driving groove 13, and the lubricating layer is used to improve the sliding smoothness of the piston part 10 in the driving groove 13 and prevent the piston part 10 from driving The inner wall of the groove 13 is worn, and the lubricating layer acts as an oil seal, which can ensure the airtightness of the driving groove 13 .

The method of using the reaction cartridge provided in this embodiment is as follows: operate the first actuating structure 33 to drive the cover 3 to slide to open the cover 3, so that the second sample injection hole 32 communicates with the first sample injection hole 212, defined The reagent tank 15 opposite to the first sample loading hole 212 is an initial reagent tank, and a detection sample is added to the initial reagent tank; the sliding cover part 3 makes the cover part 3 cover so that the second sample loading hole 32 is in line with the first sample loading port. The sample hole 212 and the initial reagent tank are not connected, and a plurality of reagent tanks 15 are communicated with the balance gas tank 4 through a plurality of air holes 213; after the sample to be detected is fully reacted in the initial reagent tank, the adapter 7 is rotated to make the transfer The two ends of connecting groove 711 communicate with the driving groove 13 and the initial reagent groove respectively; toward the direction away from the adapter 7, slide the piston member 10 to form a negative pressure environment in the driving groove 13, and draw the reagent in the initial reagent groove into the In the drive tank 13; turn the adapter 7 so that the two ends of the adapter tank 711 communicate with the drive tank 13 and another reagent tank 15 respectively, slide the piston 10 towards the direction close to the adapter 7, and place it in the drive tank 13. Inject the reagent into another reagent tank 15; according to the operation sequence of nucleic acid detection, inject the reagent in the previous reagent tank 15 into the next reagent tank 15 through the driving tank 13 until the test operation is completed; The tank 13 is filled with the initial reagent tank 15 , the cover member 3 is turned to the open state, and the final reaction reagent is extracted from the second sample injection hole 32 to realize library extraction.

As shown in Figure 12, in other embodiments, the present application provides a reaction cartridge of another structure, and the structure of the reaction cartridge provided in this embodiment is basically the same as that of the reaction cartridge described in the above-mentioned embodiments. The same, except that the reaction cartridge provided in this embodiment does not include the first fixing member 5, and the structure of the connecting member 2 in the reaction cartridge is different from that of the connecting member 2 provided in the above-mentioned embodiments. In this embodiment, the cover part 3 is fixedly connected with the cartridge main body 1 through the connecting part 2, and different from the balance gas groove 4 formed in the cover part 3 in the previous embodiment, the balance gas groove 4 is formed in the cover part 3 in this embodiment. Groove 4 is formed in connector 2 . Now only the structures in this embodiment that are different from those in the above-mentioned embodiments are described, and the same structures in this embodiment as in the above-mentioned embodiments will not be described again.

As shown in FIG. 13 , in this embodiment, the connecting member 2 further includes a connecting ring 22 , and the connecting ring 22 is disposed between the connecting plate 21 and the covering member 3 . The connecting ring 22 is a ring structure, and the connecting ring 22 extends from the periphery of the connecting plate 21 toward the covering member 3 , and the two ends of the connecting ring 22 are sealed and fixed to the connecting plate 21 and the covering member 3 respectively. The balance air groove 4 is formed by the space surrounded by the connection plate 21 and the connection ring 22 , and the balance air groove 4 communicates with a plurality of ventilation holes 213 on the connection plate 21 .

In some embodiments, the connecting ring 22 and the connecting pad 21 are integrally formed, and the connecting ring 22 and the cover member 3 are hermetically fixed by means of gluing, ultrasonic welding or laser welding. Alternatively, the connection plate 21 and the connection ring 22 can be a split structure, the connection ring 22 is made of materials such as rubber, resin or silicone grease, and the two ends of the connection ring 22 are connected to the connection plate 21 and the cover respectively by hot melting. Assembly 3 is sealed and fixed.

In this embodiment, the cover part 3 is directly fixed with the connecting part 2, and the cartridge body 1 does not need to be provided with the first fixing part 5 to fix the cover part 3, so the cartridge body 1 does not include a 5 connected to the connection part 12.

The connection plate 21 is provided with a connection hole wall 23 extending toward the cover 3 around the first connection hole 211 , and the connection plate 21 is provided with a sample injection hole wall 24 extending toward the cover 3 around the first sample injection hole 212 . Both the connection hole wall 23 and the sample injection hole wall 24 are in contact with the cover member 3 . The connection hole wall 23 and the sample injection hole wall 24 are used to isolate the balance gas tank 4 from the first connection hole 211 and the first sample injection hole 212 to ensure the normal operation of the detection process.

As shown in Fig. 14 and Fig. 15, in some embodiments, the end surfaces on both sides of the cover part 3 are planes, and only the second connection hole 31 and the second sample injection hole 32 are opened on the cover part 3. A sealing member 37 is installed in the sample hole 32 , and the second sample injection hole 32 is blocked by the sealing member 37 . Because the reaction cartridge is closed by sealing the second sample injection hole 32 with the sealing member 37 before use, it is not necessary to set the capping member 3 on the capping part 3 to rotate the capping part 3 to the capping state in this embodiment. dynamic structure.

As shown in FIG. 12 and FIG. 16 , the reaction cartridge further includes an actuation assembly 6 , and the actuation assembly 6 includes an actuation member 61 and a cover member 62 . The cover plate 62 is arranged in the balance gas tank 4 and covers all the ventilation holes 213 before using the reaction cartridge. One end of the actuating part 61 is connected with the cover part 62, and the other end of the actuating part 61 passes through the connecting plate 21, the cartridge main body 1 and the second fixing part 8 in turn and stretches out to the outside of the second fixing part 8 to cover the cover. When the plate part 62 is operated, the actuating part 61 can move back and forth toward the cover part 3 so that the cover part 62 covers or opens the plurality of ventilation holes 213 . In the embodiment shown in FIG. 12 , the connecting plate 21 , the cartridge main body 1 and the second fixing member 8 are provided with aligned through holes for the actuating member 61 to pass through, and the through holes on the cartridge main body 1 are formed in Between the bottom of a certain reagent tank 15 and the second end of the cartridge main body 1, the actuating member 61 passes through the alignment plate 21, the cartridge main body 1 and the aligned multiple through holes and the second fixing member 8 in sequence. A reagent tank 15 on the cartridge main body 1 protrudes out of the second fixing member 8 . The actuator 61 can be operated by a detection operator to control the opening and closing of the plurality of ventilation holes 213 .

When the cover member 62 covers the vent holes 213 , the balance air groove 4 is not connected to all the vent holes 213 . When the cover plate member 62 opens the vent holes 213 , the balance air groove 4 communicates with all the vent holes 213 .

The method of using the reaction cartridge provided in this embodiment is as follows: open the sealing member 37, make the second sample injection hole 32 communicate with the first sample injection hole 212, and define the reagent tank 15 facing the first sample injection hole 212 as the initial reagent Add the detection sample to the initial reagent tank; install the sealing part 37 to make the cover part 3 cover the initial reagent tank; push the actuating part 61 toward the cover part 3, and the actuating part 61 pushes the cover part 62 to open multiple Vent hole 213, make a plurality of reagent tanks 15 communicate with balance gas tank 4 through a plurality of vent holes 213; It communicates with the driving groove 13 and the initial reagent groove; slides the piston member 10 toward the direction away from the adapter 7 to form a negative pressure environment in the driving groove 13, and draws the reagent in the initial reagent groove into the driving groove 13; Connector 7, so that the two ends of the adapter groove 711 communicate with the driving groove 13 and another reagent groove 15 respectively, slide the piston 10 toward the direction close to the adapter 7, and inject the reagent in the driving groove 13 into the other reagent In the tank 15; according to the operation sequence of nucleic acid detection, the reagent in the previous reagent tank 15 is injected into the next reagent tank 15 through the driving tank 13 until the test operation is completed; the final reaction reagent is injected into the initial reagent tank through the driving tank 13, The final reaction reagent is extracted from the second sample injection hole 32 to realize library extraction.

The reaction cartridge provided in the embodiment of this application can realize sample entry and library exit with only one-step operation, with small manual workload and high detection efficiency; the reaction process of this scheme is carried out in a closed reaction cartridge, and one cartridge is for one person. There is no cross-contamination, and the pollution to the environment is avoided; the reaction cartridge of this solution is made of injection-molded plastic material, which is a disposable consumable, easy to use, and can be used with simple training for the detection operator. The reaction cartridge has built-in reagents, Users do not need to prepare additional reagents, and the cost is low.

In the second aspect, the embodiment of the present application provides a detection device, which includes the reaction cartridge described in any of the above schemes. The detection device is convenient to use and has high detection efficiency.

The reaction cartridge and detection device provided by this application can be widely used in the following fields: gene library construction; biochemical, immunological and molecular detection as a reaction container carrying reagent; pesticide residue, heavy metal and antibiotic detection as a reaction container carrying reagent; Containers hold reagents for poultry and livestock disease testing.

The above is only a specific implementation of the present application, and those skilled in the art can clearly understand that for the convenience and brevity of the description, the specific working process of the above-described systems, modules and units can refer to the foregoing method embodiments The corresponding process in , will not be repeated here. It should be understood that the protection scope of the present application is not limited thereto, and any person familiar with the technical field can easily think of various equivalent modifications or replacements within the technical scope disclosed in the application, and these modifications or replacements should cover all Within the protection scope of this application.

Claims

A reaction cartridge comprising:

Cartridge main body (1), said cartridge main body (1) is provided with a drive groove (13) and a plurality of reagent grooves (15), and the bottom of said drive groove (13) is provided with first through hole (14 ), the bottoms of the plurality of reagent tanks (15) are respectively provided with a plurality of second through holes (16);

The connector (2) includes a connection plate (21), the connection plate (21) is arranged on the first end of the cartridge body (1) and covers the reagent tank (15), the connection plate ( 21) is provided with the first sample loading hole (212) and a plurality of ventilation holes (213), the first sample loading hole (212) communicates with one of the reagent tanks (15), and the plurality of ventilation holes ( 213) communicating with multiple reagent tanks (15) respectively;

The cover (3) is arranged on the connector (2), and the cover (3) is provided with a second sample injection hole (32), and the second sample injection hole (32) is used for communicate with the first sample injection hole (212) when adding a sample;

A balance air groove (4), formed in one of the connector (2) and the cover (3), the balance air groove (4) can be selectively connected with a plurality of the ventilation holes (213 ) connected or disconnected;

The adapter (7) is slidably arranged on the second end of the cartridge main body (1), the adapter (7) is provided with an adapter groove (711), and the adapter groove (711) One end communicates with the first through hole (14), and the other end of the transfer slot (711) can slide with the adapter piece (7) to connect with the plurality of second through holes (16). One said second through hole (16) communicates;

The piston part (10) passes through the cover part (3) and the connecting part (2) and is sealed and slidably installed in the driving groove (13).
The reaction cartridge according to claim 1, wherein a first connection hole (211) is opened on the connection plate (21), a second connection hole (31) is opened on the cover (3), The first connecting hole (211), the second connecting hole (31) and the driving groove (13) are connected in sequence, and the piston member (10) passes through the first connecting hole (211) and the driving groove (13). The second connection hole (31) is slidably installed in the drive slot (13).
The reaction cartridge according to claim 1 or 2, wherein the cartridge main body (1) is cylindrical, the drive slot (13) is arranged at the center of the cartridge main body (1), and a plurality of The reagent tank (15) is arranged around the drive tank (13) in a ring shape.
The reaction cartridge according to any one of claims 1 to 3, wherein one end of the connecting plate (21) is sealed and fixed with the cartridge main body (1), and the other end of the connecting plate (21) is sealed with the cartridge main body (1). The cover (3) is slidingly connected, and the cover (3) is in the shape of a disk concentric with the cartridge main body (1), and the cover (3) can rotate around its axis and relatively Sliding on the connection plate (21), the second sample injection hole (32) can selectively communicate with or not communicate with the first sample injection hole (212) with the rotation of the cover (3) .
The reaction cartridge according to any one of claims 1 to 4, wherein the balance gas groove (4) is arranged on the cover (3) and formed between the cover (3) and the On one side surface of the connecting piece (2), the balance air groove (4) is recessed toward the side away from the connecting piece (2).
The reaction cartridge according to any one of claims 1 to 5, wherein the cover (3) can be switched between the cover state and the open state by rotation, and the balance gas groove (4) can be The rotation of the cover (3) is connected or not connected with the ventilation hole (213);

In the open state, the second sample injection hole (32) is in communication with the first sample injection hole (212), and the balance gas groove (4) is not in communication with the ventilation hole (213);

In the covered state, the second sample injection hole (32) is not in communication with the first sample injection hole (212), and the balance gas groove (4) is in communication with the ventilation hole (213).
The reaction cartridge according to any one of claims 1 to 6, wherein a first actuating structure (33) is provided on the cover (3), and the first actuating structure (33) is used for Drive the cover (3) to rotate.
The reaction cartridge according to any one of claims 1 to 7, wherein the reaction cartridge further comprises a first fixture (5), and the first fixture (5) is fixed to the cartridge main body ( 1), the connecting piece (2) and the cover piece (3) are sandwiched between the cartridge main body (1) and the first fixing piece (5).
The reaction cartridge according to any one of claims 1 to 3, wherein the connector (2) further comprises a connecting ring (22), and the connecting ring (22) is connected to the connecting plate (21) The two ends of the connecting ring (22) are sealed and fixed to the connecting plate (21) and the cover (3) respectively, and the balance air groove (4) is formed in the connecting piece (2) And it is constituted by the space surrounded by the connection plate (21) and the connection ring (22), and a sealing member (37) is installed in the second sample injection hole (32).
The reaction cartridge according to any one of claims 1 to 3 and 9, wherein the reaction cartridge further comprises an actuating assembly (6), and the actuating assembly (6) comprises an actuating member (61) and A cover piece (62), the cover piece (62) is arranged in the balance air groove (4) and is used to cover all the ventilation holes (213), and one end of the actuating piece (61) Connected with the cover part (62), the other end of the actuating part (61) passes through the connection plate (21) and the cartridge main body (1) so as to be able to move from the cartridge main body (1) The actuator (61) is externally operated, and the actuator (61) can move back and forth toward the cover (3) so that the cover (62) closes or opens the channel. The air hole (213), when the cover plate (62) covers the air hole (213), the balance air groove (4) is not connected with the air hole (213), when the cover plate ( 62) When the vent hole (213) is opened, the balance air groove (4) communicates with the vent hole (213).
The reaction cartridge according to any one of claims 1 to 10, wherein a second actuating structure (721) is provided at the end of the adapter (7) away from the cartridge body (1), the The second actuating structure (721) is used to drive the adapter (7) to rotate.
The reaction cartridge according to any one of claims 1 to 11, wherein the reaction cartridge further comprises a second fixture (8), and the second fixture (8) is fixed to the cartridge main body ( 1), the adapter piece (7) is rotatably installed between the cartridge main body (1) and the second fixing piece (8).
The reaction cartridge according to claim 12, wherein a rotary assembly (9) is arranged between the adapter (7) and the second fixing member (8).
A detection device, comprising the reaction cartridge according to any one of claims 1 to 13.