CN107570243A - A kind of hand gets rid of formula multi-target analysis chip and its application method and application - Google Patents
A kind of hand gets rid of formula multi-target analysis chip and its application method and application Download PDFInfo
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- CN107570243A CN107570243A CN201710643353.7A CN201710643353A CN107570243A CN 107570243 A CN107570243 A CN 107570243A CN 201710643353 A CN201710643353 A CN 201710643353A CN 107570243 A CN107570243 A CN 107570243A
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Abstract
The invention belongs to technical field of biological, more particularly to a kind of hand used in being checked or analyzed of biochemical reaction gets rid of formula multi-target analysis chip.The chip includes base material and envelope material；Base material, which is provided with, is easy to hand-held hand-held region and multiple reaction zones, reaction zone offers multiple reacting holes and wavy first groove, reacting hole is corresponded with the trough of the first groove and is connected by the second groove, reacting hole is located at the outside of corresponding trough, the both ends of first groove respectively offer an adding mouth, hold region positioned at side relative with the first groove and away from reacting hole；Timber-used is sealed in capping reacting hole, the first groove and the second groove, and seals material and offers the well corresponding with adding mouth.Present invention also offers the application method of the chip and application.The chip of the present invention is got rid of during can sample be distributed into different reacting holes automatically and is mutually isolated by hand, had a wide range of applications in biological detection or medical inspection field without external device.
Description
Technical field
The invention belongs to technical field of biological, more particularly to a kind of use in being checked or analyzed of biochemical reaction
Hand gets rid of formula multi-target analysis chip and its application method and application.
Background technology
Biochemical analysis, immunoassay and the foranalysis of nucleic acids commonly used in biochemistry detection field, such as traditional in-vitro diagnosis,
In the presence of the needs that multiple indexs of same sample are carried out with parallel parsing.Tradition realizes that the method for multi-target analysis as will
Sample is assigned in multiple test tubes, and added with the reagent of specific indexes detection in each test tube, multi objective is realized with multiple test tubes
Analysis.This method is although simple and reliable, is suitable for the batch-automated operation of manipulator, but for some field assays
Occasion is but not suitable for, because its equipment used is huge, expensive, is unsatisfactory for quick, portable, the inexpensive needs of Site Detection.
The huge basic reason of traditional multi-target analysis equipment volume is that it realizes sample based on traditional mechanical structure
Distribution sample-adding, micro-fluidic chip is currently based on to automate and realizes liquid transporting, distribution, so as to realize that Portable multi-index is analyzed
It is the important development trend of analysis field.Microflow control technique is that one kind controls microfluid by the structure such as microchannel and microcavity body
Complete a kind of technology of various biological and chemical processes.Cell culture is had been widely used at present, cytositimulation, cell split
In the correlative studys such as solution, nucleic acid extraction, nucleic acid amplification reaction, immunoassay, environmental monitoring.The micro-fluidic core that these analyses need
Piece usually contains multiple, independent, homogeneous reaction tank and carrys out the separately or combined reaction completed for different samples or index.Because
Reagent needed for different reactions is different, so often needing to configure different reaction systems in differential responses pond, such as enzyme, substrate
Deng.Multi objective biochemical reaction or detection are completed with needed for automaticity height, reagent consumption, reaction using micro-fluidic chip
The features such as cost is low.
How to automate and realize that sample is the pass for realizing multi-target analysis toward the distribution in the reacting hole of different analysis indexes
Key step.It is combined using Micro-flow pipe structure and centrifugal force and realizes that the transport of sample and distribution are in current micro-fluidic research
A kind of common method.As patent US6627159 using centrifugation by the way of sample is assigned to pipeline two if reaction tank in, so
The metal base with pressure sensitive adhesive is allowed to deform using external equipment afterwards, by the emptying rate of liquid in the pipeline of connected reaction pond, finally
Realize and the liquid in differential responses hole is distributed and isolated.Patent US6919058B2, US20030166265A1,
US6752961B2, WO2004083108A1, WO2013045631A1 etc., using the chip of optical-disk type structure, by centrifugal force or
Centrifugal force the mode such as is combined by the fluid distribution in serial pipe into the reacting hole on the outside of pipeline with capillary force.More than
Method preferably realizes in multi-target analysis the problem of sample distributes, but needs special equipment, auxiliary such as centrifuge
Operation could be completed by helping, and can't fully be met in some Site Detections to inexpensive and portable needs.
The content of the invention
(1) technical problems to be solved
The technical problem to be solved in the present invention is to provide a kind of hand to get rid of formula multi-target analysis chip, and the chip is set without the external world
Standby, being got rid of by hand can be automatically assigned in different reacting holes and be mutually isolated by example reaction liquid.
Invention also provides application method and the application that the hand gets rid of formula multi-target analysis chip, chip user
Just, have a wide range of applications in the numerous areas such as biological detection or medical inspection.
(2) technical scheme
In order to solve the above-mentioned technical problem, the invention provides a kind of hand to get rid of formula multi-target analysis chip, and it includes：
Base material, the base material, which is provided with, is easy to hand-held hand-held region and multiple reaction zones, and the reaction zone offers multiple
Reacting hole and wavy first groove, the trough of the reacting hole and first groove correspond and pass through second
Groove is connected, and the reacting hole is located at the outside of corresponding trough, and the both ends of first groove respectively offer an adding mouth,
The hand-held region is positioned at side relative with first groove and away from the reacting hole；And
Feng Cai, the envelope timber-used in covering reacting hole, the first groove and second groove, and the envelope material offer with
The corresponding well of the adding mouth.
Further, the hand-held region is provided with a plurality of raised line parallel with first groove.
Further, the base material and/or envelope material are transparent material.
Further, the base material is made up of the one or more in high molecular polymer, glass, silicon or metal.
Preferably, the high molecular polymer is polymethyl methacrylate, makrolon, polypropylene or poly dimethyl silicon
Any of oxygen alkane.
Further, the envelope material is adhesive tape (being preferably one side glue) or the tabula rasa of smooth surface.
Further, first groove is sequentially connected with by multiple V-shape grooves and formed.
Present invention also offers the application method that a kind of above-mentioned hand gets rid of formula multi-target analysis chip, it comprises the following steps：
Step 1：Different reaction substrates is fixed in different reacting holes respectively, i.e., these reaction substrates are not respectively for
Same index；
Step 2：By the base material and envelope material fitting；
Step 3：Sample is passed sequentially through into the well and adding mouth and injects first groove, then seals up described add
Sample hole (preferably seals well) by gluing mode；
Step 4：The hand-held region is held, inertia force is then produced in a manner of whipping from top to bottom, by the sample
It is assigned to by the first groove in different reacting holes, being originally present in the air in reacting hole can enter in the first groove with isolation
Different reacting holes；Sample is completed toward the distribution in reacting hole with regard to this, because securing different fingers in differential responses hole in advance
Reaction substrate required for mark detection, thus multi-target analysis can be realized.
Further, the mode being bonded described in step 2 is any in hot pressing, glue sticking, laser welding or ultra-sonic welded
Kind.
Preferably, packaged hand gets rid of rectangular formula multi-target analysis chip, triangle or sector structure.
The present invention still further provides above-mentioned hand and get rid of formula multi-target analysis chip in biological detection or medical inspection
Using.
Further, the biological detection is accounting amplified reaction.
(3) beneficial effect
The above-mentioned technical proposal of the present invention has the advantages that：
The hand of the present invention gets rid of formula multi-target analysis chip, and base material is provided with the first groove, the second groove and reacting hole, passed through
Envelope material seals base material, and the fluid passage of sealing is formd on base material；The side relative with reaction zone is additionally provided with hand-held on base material
Region, the hand-held region is held, base material described in whipping produces inertia force from top to bottom, what sample can be made up of the groove
Fluid passage enters in reacting hole, secures the reaction substrate for different indexs in advance in differential responses hole, thus can be with
Realize multi-target analysis.The hand of the present invention gets rid of formula multi-target analysis chip without external device, and being got rid of by hand can be anti-by sample
Answer liquid distribute automatically into different reacting holes in and be mutually isolated.
Thus, hand of the invention gets rid of that formula multi-target analysis chip is easy to use, and each reaction zone can distinguish a kind of sample,
Due to there is provided multiple reaction zones, thus several samples can be disposably distinguished, and simple to operate, independent of Instrumental Analysis, significantly
Testing cost is reduced, and because the reaction environment of base material and envelope material composition is closed, is effectively prevented during sample analysis
The pollution of environment.
Brief description of the drawings
Fig. 1 is the structural representation that hand described in the embodiment of the present invention 1 gets rid of formula multi-target analysis chip；
Fig. 2 is base material described in the embodiment of the present invention 1 and the fractionation structural representation for sealing material；
Fig. 3 is the view of reaction system before the reaction described in the embodiment of the present invention 2；
Fig. 4 is the view of reaction system after the reaction described in the embodiment of the present invention 2；
Wherein, 1, base material；2nd, Feng Cai；3rd, region is held；4th, reaction zone；5th, reacting hole；6th, the first groove；7th, the first groove
Trough；8th, the second groove；9th, adding mouth；10th, well；11st, raised line；12nd, V-shape groove；
13-1, the state of the reaction system of Escherichia coli Specific PCR primers before the reaction is embedded in advance, be purple；
13-2, the state of the reaction system of Escherichia coli Specific PCR primers after the reaction is embedded in advance, for green；
14-1, the state of the reaction system of staphylococcus aureus specific PCR primer before the reaction is embedded in advance, be
Purple；
14-2, the state of the reaction system of staphylococcus aureus specific PCR primer after the reaction is embedded in advance, be
Purple.
Embodiment
Embodiments of the present invention are described in further detail with reference to the accompanying drawings and examples.Following examples are used for
Illustrate the present invention, but can not be used for limiting the scope of the present invention.
In the description of the invention, unless otherwise indicated, " multiple " are meant that two or more.Term " on ",
" under ", "left", "right", " interior ", " outer ", " front end ", " rear end ", " head ", the orientation of the instruction such as " afterbody " or position relationship be
Based on orientation shown in the drawings or position relationship, it is for only for ease of the description present invention and simplifies description, rather than instruction or dark
Show that the device of meaning or element there must be specific orientation, with specific azimuth configuration and operation, thus it is it is not intended that right
The limitation of the present invention.In addition, term " first ", " second ", " the 3rd " etc. be only used for describe purpose, and it is not intended that instruction or
Imply relative importance.
In the description of the invention, it is necessary to illustrate, unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " should be interpreted broadly, for example, it may be being fixedly connected or being detachably connected, or be integrally connected；Can
To be to be joined directly together, can also be indirectly connected by intermediary.For the ordinary skill in the art, tool can be regarded
Body situation understands the concrete meaning of above-mentioned term in the present invention.
Embodiment 1
As depicted in figs. 1 and 2, present embodiments provide a kind of hand and get rid of formula multi-target analysis chip, it includes base material 1 and envelope
Material 2, by base material 1 and envelope material 2 it is packaged after, the analysis chip is in sector structure；Base material 1, which is provided with, is easy to hand-held hand-held region 3
With two reaction zones 4, each reaction zone 4 offers multiple reacting holes 5 and wavy first groove 6, the reacting hole 5
Correspond with the trough 7 of first groove 6 and be connected by the second groove 8, the reacting hole 5 is located at corresponding trough 7
Outside, the both ends of first groove 6 respectively offer an adding mouth 9, and the hand-held region 3 is located at and first groove
6 sides relative and away from the reacting hole 5；Envelope material 2 is used to cover the reacting hole 5, the first groove 6 and the second groove 8, and
The envelope material 2 offers the well 10 corresponding with the adding mouth 9, and (well 10 can be opened before being bonded with base material 1
If it can also be opened up after being bonded with base material 1 using sharp object；The envelope material 2 of the present embodiment is due to for flexible structure, therefore
Selection opens up well 10 using sharp object after being bonded with base material 1 in adding mouth position).
The hand-held region 3 is provided with a plurality of raised line 11 parallel with first groove 6.The base material 1 and envelope material 2 are
Transparent material.Base material 1 can be made up of the one or more in high molecular polymer, glass, silicon or metal, the base material of the present embodiment
1 is preferably made up of polymethyl methacrylate and glass according to routine techniques.Seal the one side glue that material 2 is smooth surface.First is recessed
By multiple head and the tail of V-shape groove 12, sequentially connection forms groove 6.
The present embodiment additionally provides the application method that a kind of hand gets rid of formula multi-target analysis chip, and it comprises the following steps：
Step 1：Different reaction substrates is fixed in different reacting holes 5 respectively, i.e., these reaction substrates are directed to respectively
Different indexs；
Step 2：Base material 1 and envelope material 2 are bonded in a manner of hot pressing；
Step 3：Sample is passed sequentially through into the well 10 and adding mouth 9 and injects first groove 6, then passes through glue
Viscous mode seals well 10；
Step 4：The hand-held region 3 is held, inertia force is then produced in a manner of whipping from top to bottom, by sample by first
Groove 6 is assigned in different reacting holes 5, and being originally present in the air in reacting hole 5 can enter in the first groove 6 to isolate not
Same reacting hole 5；Sample is completed toward the distribution in reacting hole 5 with regard to this, because securing different fingers in differential responses hole 5 in advance
Reaction substrate required for mark detection, thus multi-target analysis can be realized.
In summary, the hand of the present embodiment gets rid of formula multi-target analysis chip without external device, and being got rid of by hand can be by sample
During product reaction solution distributes into different reacting holes automatically and it is mutually isolated.Thus easy to use, each reaction zone can distinguish one
Kind of sample, due to there is provided multiple reaction zones, thus several samples can be disposably distinguished, and it is simple to operate, independent of instrument point
Analysis, testing cost is greatly reduced, and because the reaction environment of base material and envelope material composition is closed, had during sample analysis
Effect prevents the pollution of environment.
Embodiment 2
Present embodiments provide a kind of above-mentioned hand and get rid of application of the formula multi-target analysis chip in nucleic acid amplification reaction.
First, Escherichia coli Specific PCR primers and staphylococcus aureus specific PCR primer are embedded in two instead
In the differential responses hole 5 for answering area 4, two kinds of primers are arranged at intervals, and multi-target analysis chip is prepared into after capped envelope material 2.
Then, reaction reagent is visualized using from the PCR bought on the market, prepares DNA profiling containing genome of E.coli
PCR reaction systems (final concentration of 80 copy/μ l of template), by the reaction system prepared from left (or right) well 10 plus
Enter to the first groove 6 of chip internal, closed well 10 with sealed membrane.One hand clutches the hand-held region 3 of chip, from upper
Down exert oneself whipping chip, the PCR reaction systems in the first groove 6 are centrifuged into reacting hole 5 by inertia, with advance embedding
Primer contact lysis, now the color of reaction system is purple (as shown in figure 3,13-1 is has embedded Escherichia coli in advance
The state of the reaction system of Specific PCR primers before the reaction, it is purple；14-1 is special to have embedded staphylococcus aureus in advance
The state of the reaction system of specific PCR primers before the reaction, it is purple).
Finally, the chip containing reaction system is put into PCR instrument and reacted.
Fig. 4 is the result of the chip after the reaction, it is seen then that has embedded the reaction of Escherichia coli Specific PCR primers in advance
System becomes for reacted green 13-2 from the purple 13-1 before reaction, has embedded staphylococcus aureus specific in advance
14-1 and 14-2's reaction system of PCR primer after reaction does not change before the reaction.
Therefore, the PCR visualizing agents of market purchase are coordinated using the multi-target analysis chip, can disposably be carried out a variety of
Reaction, and it is simple to operate, independent of Instrumental Analysis, testing cost is greatly reduced, and because reaction environment is closed, effectively
The pollution for preventing environment.
Embodiments of the invention provide for the sake of example and description, and are not exhaustively or by this to send out
It is bright to be limited to disclosed form.Many modifications and variations are obvious for the ordinary skill in the art.Choosing
Select and describe embodiment and be to more preferably illustrate the principle and practical application of the present invention, and make one of ordinary skill in the art
It will be appreciated that the present invention is so as to designing the various embodiments with various modifications suitable for special-purpose.
Claims (10)
1. a kind of hand gets rid of formula multi-target analysis chip, it is characterised in that including：
Base material, the base material, which is provided with, is easy to hand-held hand-held region and multiple reaction zones, and the reaction zone offers multiple reactions
The trough of hole and wavy first groove, the reacting hole and first groove corresponds and passes through the second groove
It is connected, the reacting hole is located at the outside of corresponding trough, and the both ends of first groove respectively offer an adding mouth, described
Hand-held region is positioned at side relative with first groove and away from the reacting hole；
Feng Cai, the envelope timber-used in covering reacting hole, the first groove and second groove, and the envelope material offer with it is described
The corresponding well of adding mouth.
2. hand according to claim 1 gets rid of formula multi-target analysis chip, it is characterised in that the hand-held region is provided with a plurality of
The raised line parallel with first groove.
3. hand according to claim 1 gets rid of formula multi-target analysis chip, it is characterised in that the base material and/or envelope material are
Transparent material.
4. hand according to claim 1 gets rid of formula multi-target analysis chip, it is characterised in that the base material is by high molecular polymerization
One or more in thing, glass, silicon or metal are made.
5. hand according to claim 1 gets rid of formula multi-target analysis chip, it is characterised in that the envelope material be adhesive tape or
The tabula rasa of smooth surface.
6. hand according to claim 1 gets rid of formula multi-target analysis chip, it is characterised in that first groove is by multiple V
Font groove, which is sequentially connected with, to be formed.
7. a kind of any one of claim 1~6 hand gets rid of the application method of formula multi-target analysis chip, it is characterised in that bag
Include following steps：
Step 1：Different reaction substrates is fixed in different reacting holes respectively；
Step 2：By the base material and envelope material fitting；
Step 3：Sample is passed sequentially through into the well and adding mouth and injects first groove, then seals up the sample-adding
Hole；
Step 4：The hand-held region is held, inertia force is then produced in a manner of whipping from top to bottom, by the sample by the
One groove is assigned in different reacting holes.
8. application method according to claim 7, it is characterised in that the mode being bonded described in step 2 is hot pressing, gluing
Connect, any of laser welding or ultra-sonic welded.
9. any one of claim 1~6 hand gets rid of application of the formula multi-target analysis chip in biological detection or medical inspection.
10. application according to claim 9, it is characterised in that the biological detection is accounting amplified reaction.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710643353.7A CN107570243A (en) | 2017-07-31 | 2017-07-31 | A kind of hand gets rid of formula multi-target analysis chip and its application method and application |
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| CN201710643353.7A CN107570243A (en) | 2017-07-31 | 2017-07-31 | A kind of hand gets rid of formula multi-target analysis chip and its application method and application |
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| Publication Number | Publication Date |
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| CN107570243A true CN107570243A (en) | 2018-01-12 |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102886280A (en) * | 2012-08-28 | 2013-01-23 | 博奥生物有限公司 | Microfluidic chip and application thereof |
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2017
- 2017-07-31 CN CN201710643353.7A patent/CN107570243A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102886280A (en) * | 2012-08-28 | 2013-01-23 | 博奥生物有限公司 | Microfluidic chip and application thereof |
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Application publication date: 20180112 |