WO2023119820A1 - Fermented composition for feed, and method for raising livestock - Google Patents

Fermented composition for feed, and method for raising livestock Download PDF

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Publication number
WO2023119820A1
WO2023119820A1 PCT/JP2022/038769 JP2022038769W WO2023119820A1 WO 2023119820 A1 WO2023119820 A1 WO 2023119820A1 JP 2022038769 W JP2022038769 W JP 2022038769W WO 2023119820 A1 WO2023119820 A1 WO 2023119820A1
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livestock
vitamin
fermented composition
composition
belonging
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PCT/JP2022/038769
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French (fr)
Japanese (ja)
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良紀 松浦
晋輔 岸田
英人 鳥居
拓三 水津
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万田発酵株式会社
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Definitions

  • the present invention relates to a fermented composition for livestock feed and a method for raising livestock using the fermented composition.
  • Livestock such as pigs are sometimes fed with antibiotics such as avilamycin, enramycin, virginiamycin and colistin for growth purposes in addition to feeding.
  • antibiotics such as avilamycin, enramycin, virginiamycin and colistin for growth purposes in addition to feeding.
  • the use of antibiotics for growth purposes involves continuous use in the feed. Dosages are generally used that are low relative to the amount of antibiotics used for therapeutic purposes. Usage standards such as dosage are determined for each antibiotic.
  • antibiotics are given to livestock for the purpose of growth is to prevent diarrhea in livestock by suppressing the generation of toxins and putrefactive substances produced by harmful bacteria in the intestines and preventing damage to villi and crypts. It is intended to suppress and promote healthy growth of livestock.
  • the present inventors are earnestly researching the use and effects of fermented compositions obtained by fermenting and aging specific multiple types of raw materials from fruits, grains, seaweeds, and vegetables for a long period of time.
  • the fermented composition increases the production of short-chain fatty acids in the intestines of animals, hypothesized whether it could replace antibiotics given to livestock, and through trial and error and various experiments, the present invention. It has reached
  • the present invention is 1 selected from apples, persimmons, bananas, pineapples, akebi, Actinidia, figs, wild strawberries, strawberries, wild grapes, grapes, mountain vines, peaches, plums, blueberries, and raspberries belonging to fruits.
  • the object is to provide a fermented plant composition for feed (for livestock) containing a fermented composition as a main raw material.
  • the fermented composition may be characterized by increasing specific short chain fatty acids in the gut of livestock.
  • a fermented plant composition for use (for livestock).
  • the present invention provides an antibiotic substitute containing the prebiotic food according to claim 1 or 2, which is characterized by suppressing diarrhea in domestic animals, which uses the fermented composition as a main raw material.
  • One or two fruits selected from apples, persimmons, bananas, pineapples, akebi, actinidia, figs, wild strawberries, strawberries, wild grapes, grapes, wild grapes, peaches, plums, blueberries, raspberries, and loquats.
  • amino acid composition in 100 g, isoleucine: 30-200 mg, Leucine: 50-400 mg, Lysine: 20-200 mg, methionine: 10-150 mg, Cystine: 10-100 mg, Phenylalanine: 30-250 mg, Tyrosine: 20-200 mg, Threonine: 40-200 mg, tryptophan: 1-100 mg, Valine: 30-300 mg, Histidine: 10-200 mg, Arginine: 40-400 mg, Alanine: 50-300 mg, Aspartic acid: 100-600 mg, Glutamic acid: 100-1200 mg, Glycine: 30-300 mg, proline: 40-400 mg, Serine: 30-300 mg.
  • a method of raising livestock by feeding livestock a prebiotic additive for feed containing the fermented composition as a main raw material may also be used.
  • the fermented composition may be characterized by increasing specific short chain fatty acids in the gut of livestock.
  • a livestock breeding method may be adopted in which antibiotics are not administered or the amount used is reduced by feeding livestock with a prebiotic additive for feed, the main raw material of which is the fermented composition.
  • the fermented composition may be characterized by increasing specific short chain fatty acids in the gut of livestock.
  • the administration group of the present fermented composition maintained the same level of weight gain of livestock as compared to the antibiotic administration group, and the blood, the length and weight of the intestinal tract, and the villous tissue showed significant changes. No abnormal findings were observed, and diarrhea symptoms (stool score) were similar.
  • the present inventor focused on the present fermented composition and conducted an experiment to determine whether or not it affects the growth of livestock (especially edible pigs) under livestock conditions in which antibiotics are not administered. .
  • This fermented composition contains apples, persimmons, bananas, pineapples, akebi, actinidia, figs, wild strawberries, strawberries, wild grapes, grapes, wild grapes, peaches, plums, blueberries, raspberries, One or two or more kinds selected from loquat, and one or more kinds selected from citrus navel, hassaku, unshu orange, summer orange, orange, iyokan, kumquat, yuzu, kabosu, pomelo, ponkan, lemon, and lime Two or more kinds, one or more kinds selected from burdock, carrot, garlic, lotus root, and lily bulb belonging to root crops, and brown rice, glutinous rice, white rice, millet, corn, wheat, 1 or 2 or more selected from barley, foxtail millet, and millet; One or two or more selected from kelp, wakame, hijiki, green laver, and kawanori belonging to seaweeds, one or two or more selected from
  • the fermentation and maturation period was 3 years and 3 months or longer.
  • no additives, heat, or water were artificially added, and the fermentation was carried out in a stationary state for three years or more.
  • 1 or 2 types were selected from each type, and 15 types of raw materials were selected and fermented (this fermented composition) was used.
  • the introduced pigs used in this experiment were 23-day-old suckling piglets (crossbreed).
  • rats were fed with a general feed containing antibiotics (control group) and a feed (test group) in which the antibiotic was replaced with FBP (this fermented composition) for comparison.
  • the same mixed feed for piglets was used in both the control group and the test group.
  • 40 ppm of aviramycin was added as an antibiotic to the control group, and no antibiotic was added to the test group.
  • 0.125% of the present fermented composition was added to the test plot, and the present fermented composition was not added to the control plot.
  • the feeding period in this experiment was from 23 days old to 50 days old.
  • the breeding environment was a single cage, and the feeding method was free drinking water and constant feeding.
  • the mixed feed for piglets used a mixed feed suitable for the breeding stage.
  • Avilamycin antibiotic
  • FBP this fermented composition
  • sampling sites and measurement items is "feces, feces score/IgA”, "intravenous blood, blood biochemical analysis”, “cecal venous blood, short chain fatty acid analysis”, “cecal contents, short chain fatty acid analysis”, and “small intestine/large intestine, organ measurement/villus length/crypt depth”.
  • a score of 0 was assigned to a good stool, which was regarded as a stool.
  • Soft stool was scored as 1, diarrheal stool as score 2, watery stool as score 3, and more water-soluble diarrhea as score 4. The higher the score, the worse the stool.
  • FIG. 1 (left) is a graph showing the growth performance of edible pigs.
  • control group the body weight of edible pigs bred with a general feed containing antibiotics (control group) and a feed in which the antibiotic was replaced with FBP (this fermented composition) (test group) was measured and graphed. bottom.
  • the vertical axis is weight (kg), and the horizontal axis is age after birth (days). Both the control group and the test group showed similar weight gain over time.
  • the feed conversion rate is calculated as [feed intake (or consumption) (kg) / livestock product production (kg)].
  • the feed conversion rate is a unitless number.
  • the feed conversion rate increases as the postnatal age increases, reaching a peak at the 37th to 44th day postnatal age, and tends to decrease slightly at the 44th to 55th postnatal age.
  • the feed conversion rate showed almost the same values in the control plot and the test plot.
  • the feed conversion rate is the amount of feed consumed (or consumed) required for production of 1 kg of livestock product. This indicates that the higher the efficiency, the better.
  • Figure 2 shows data obtained by observing the intestinal environment at specific times in the control and test plots.
  • the left graph shows fecal scores at specific age periods.
  • the fecal score evaluation method is as described above.
  • the fecal score was good in both the control group and the test group, and the intestinal environment was stable during the period.
  • Right graph shows fecal IgA scores at specific ages (23 days, 31 days, 50 days). No change in resting intestinal immunity was observed.
  • the fecal IgA concentration correlates with the immune status in the intestinal tract.
  • the fecal IgA concentration is high due to infectious diseases, and conversely, if the fecal IgA concentration is extremely low, the risk of infectious diseases increases. Since the values were low and stable in both groups, it is thought that this indicates that the intestinal environment was relatively stable.
  • Fig. 3 is a graph showing short-chain fatty acid concentrations in cecal contents and cecal venous blood in the control and test groups. Acetic acid, propionic acid, and n-butyric acid were measured as short-chain fatty acids. Both the cecal contents and the venous blood showed no significant change in the composition ratio of overall short-chain fatty acids with FBP feeding in both the control group and the test group, but the short-chain fatty acid concentration was high in the test group. A trend was observed.
  • the intestinal mucosa plays an intestinal barrier function to prevent invasion of pathogens.
  • Butyric acid and propionic acid have the function of maintaining the intestinal mucosa and enhancing the intestinal barrier function.
  • butyric acid is the most important energy source for intestinal epithelial cells, and has been reported to promote the metabolism of the intestinal epithelium and the peristaltic movement of the intestinal tract.
  • Fig. 4 is a table showing the shape of the gastrointestinal tract and blood biochemical examination of edible pigs.
  • Anatomy such as small intestine (length, weight), large intestine (length, weight), digestive absorption (villus height ( ⁇ m), crypt depth ( ⁇ m), villus/crypt depth) in control and test groups
  • villus height ⁇ m
  • crypt depth ⁇ m
  • villus/crypt depth a table showing the shape of the gastrointestinal tract and blood biochemical examination of edible pigs.
  • Fig. 5 is an explanatory diagram of the effect mechanism of the present fermented composition on edible pigs.
  • harmful bacteria proliferate in the digestive tract due to stress or disease on the living body, and these harmful bacteria produce toxins and putrefactive substances that damage the inside of the digestive tract. Specifically, it damages the villi and crypts responsible for digestive and absorption functions, resulting in diarrhea.
  • antibiotics were given to livestock during breeding to remove the so-called harmful bacteria in the digestive tract, prevent diarrhea, etc., and promote growth.
  • harmful bacteria there were some bacteria that were resistant to antibiotics.
  • the fermented composition according to the present invention could be used as an antibiotic substitute material for raising livestock because the same effect can be obtained even when antibiotics are not given. That is, it is believed that the fermented composition increases useful bacteria in the gastrointestinal tract of livestock, promotes the production of short-chain fatty acids from these useful bacteria, promotes the utilization of nutrients, and can achieve a favorable intestinal environment. . Since the same effect can be obtained even when antibiotics are not given, the fermented composition according to the present invention was able to be used as an antibiotic substitute material for raising livestock.

Abstract

[Problem] To provide a fermented vegetable composition material that is usable as a substitute for an antibiotic in raising livestock. [Solution] A substitute material for an antibiotic to be used in raising livestock, said substitute material comprising, as a main ingredient, a fermented composition obtained by fermenting and maturing two or more materials selected from among fruits, citrus fruits, burdock root and carrot belonging to root crops, cereals, sesame seeds, seaweeds and sugars, and having a characteristic of increasing specific short-chain fatty acids in the intestines.

Description

飼料用の発酵組成物、家畜の飼育方法Fermented composition for feed, method for raising livestock
　本発明は、家畜飼料用の発酵組成物、及び、当該発酵組成物を用いた家畜の飼育方法に関する。 The present invention relates to a fermented composition for livestock feed and a method for raising livestock using the fermented composition.
　食用豚などの家畜の飼育には、飼料の給餌の他に成長目的のためのアビラマイシン、エンラマイシン、バージニアマイシン、コリスチンなどの抗生物質を与えていることがある。
成長目的での抗生物質の使用は、飼料に混ぜて継続的に使用されるものである。投与量は、一般的には治療目的の抗生物質の使用量に対して低用量で用いられている。
投与量など使用基準は、抗生物質ごとに定められている。 Livestock such as pigs are sometimes fed with antibiotics such as avilamycin, enramycin, virginiamycin and colistin for growth purposes in addition to feeding.
The use of antibiotics for growth purposes involves continuous use in the feed. Dosages are generally used that are low relative to the amount of antibiotics used for therapeutic purposes.
Usage standards such as dosage are determined for each antibiotic.
　家畜の飼育において、成長目的で抗生物質を家畜に与える理由は、腸内の有害菌が産生する毒素・腐敗物の発生を抑えることで絨毛・陰窩の損傷を防ぐことにより、家畜の下痢を抑制して、健全な家畜の成長を図るものである。 In livestock breeding, the reason why antibiotics are given to livestock for the purpose of growth is to prevent diarrhea in livestock by suppressing the generation of toxins and putrefactive substances produced by harmful bacteria in the intestines and preventing damage to villi and crypts. It is intended to suppress and promote healthy growth of livestock.
　しかし、この抗生物質に対する耐性菌が出現することで、抗生物質の効果が弱くなり、毒素・腐敗物の発生を防ぐことができず、家畜の下痢を発生させてしまう。また、昨今の自然食ブームのなかでも、抗生物質を与えた家畜など消費者の購入を躊躇させるなどの影響もある。　 However, the emergence of antibiotic-resistant bacteria weakens the effectiveness of antibiotics, making it impossible to prevent the generation of toxins and putrefactive substances, resulting in diarrhea in livestock. In addition, even in the recent natural food boom, there is also an impact such as making consumers hesitate to purchase livestock given antibiotics.　
　本発明者らは、果物、穀類、海藻類、野菜から特定の複数種類の原材料を長期間の発酵熟成させた発酵組成物の利用や効果について鋭意研究している。当該発酵組成物が動物の腸内での短鎖脂肪酸の産生が増加することを見出し、家畜に与える抗生物質を代替できないか否かの仮説を立てて、試行錯誤や各種実験を経て、本発明に至ったものである。 The present inventors are earnestly researching the use and effects of fermented compositions obtained by fermenting and aging specific multiple types of raw materials from fruits, grains, seaweeds, and vegetables for a long period of time. We found that the fermented composition increases the production of short-chain fatty acids in the intestines of animals, hypothesized whether it could replace antibiotics given to livestock, and through trial and error and various experiments, the present invention. It has reached
　本発明がその技術的課題を解決するために用いる技術的手段としては、次のようなものである。 The technical means used by the present invention to solve the technical problems are as follows.
　すなわち、本発明は、果実類に属するリンゴ、柿、バナナ、パインアップル、アケビ、マタタビ、イチジク、野いちご、いちご、山ぶどう、ぶどう、山挑、もも、梅、ブルーベリー、ラズベリーから選ばれる１種または２種以上のものと、かんきつ類に属するネーブル、ハッサク、温州みかん、夏みかん、オレンジ、伊予柑、きんかん、ゆず、カボス、ザボン、ポンカン、レモン、ライムから選ばれる１種または２種以上のものと、根菜類に属するゴボウ、ニンジン、ニンニク、レンコン、ゆり根から選ばれる１種または２種以上のものと、穀類に属する玄米、もち米、白米、きび、とうもろこし、小麦、大麦、あわ、ひえから選ばれる１種または２種以上のものと、豆・ゴマ類に属する大豆、黒豆、黒ゴマ、白ゴマ、あずき、くるみから選ばれる１種または２種以上のものと、海草類に属するコンブ、ワカメ、ヒジキ、あおのり、かわのりから選ばれる１種または２種以上のものと、糖類に属する黒糖、果糖、ぶどう糖から選ばれる１種または２種以上のものと、はちみつ、澱粉、きゅうり、しそ、セロリから選ばれる１種または２種以上のものとを、発酵、熟成させることで得られ、次の成分及びアミノ酸組成からなる、
主成分について、１００ｇ当たり、下記を含む、
　水分：５．０ｇ～５０．０g、
　タンパク質：０．５ｇ～１０．０ｇ、
　脂質：０．０５ｇ～１０．００ｇ、
　炭水化物（糖質）：３０．０ｇ～７５．０ｇ、
　炭水化物（繊維）：０．１ｇ～５．０ｇ、
　灰分：０．５ｇ～５．０g、
　β－カロチン：１０μｇ～１５０μｇ、
　ビタミンＡ効力：１０ＩＵ～１００ＩＵ、
　ビタミンＢ1：０．０１ｍｇ～０．５０ｍｇ、
　ビタミンＢ2：０．０１ｍｇ～０．５０ｍｇ、
　ビタミンＢ6：０．０１ｍｇ～０．５０ｍｇ、
　ビタミンＥ：１０．０ｍｇ以下、
　ナイアシン：０．１ｍｇ～６．０ｍｇ、
　カルシウム：５０ｍｇ～９００ｍｇ、
　リン：２００ｍｇ以下、
　鉄：１．０ｍｇ～５．０ｍｇ、
　ナトリウム：２０ｍｇ～３００ｍｇ、
　カリウム：３００ｍｇ～１０００ｍｇ、
　マグネシウム：４０ｍｇ～２００ｍｇ、
　食塩相当量：０．０５ｇ～１．００ｇ、
　銅：７．０ｐｐｍ以下。
アミノ酸組成について、１００ｇ中、
　イソロイシン：３０～２００ｍｇ、
　ロイシン：５０～４００ｍｇ、
　リジン：２０～２００ｍｇ、
　メチオニン：１０～１５０ｍｇ、
　シスチン：１０～１００ｍｇ、
　フェニルアラニン：３０～２５０ｍｇ、
　チロシン：２０～２００ｍｇ、
　スレオニン：４０～２００ｍｇ、
　トリプトファン：１～１００ｍｇ、
　バリン：３０～３００ｍｇ、
　ヒスチジン：１０～２００ｍｇ、
　アルギニン：４０～４００ｍｇ、
　アラニン：５０～３００ｍｇ、
　アスパラキン酸：１００～６００ｍｇ、
　グルタミン酸：１００～１２００ｍｇ、
　グリシン：３０～３００ｍｇ、
　プロリン：４０～４００ｍｇ、
　セリン：３０～３００ｍｇ。
発酵組成物を主原料とする飼料用（家畜用）の植物発酵組成材を提供するものである。この発酵組成物は、家畜の腸内で特定の短鎖脂肪酸を増加させる特徴のあるものとしても良い。 That is, the present invention is 1 selected from apples, persimmons, bananas, pineapples, akebi, Actinidia, figs, wild strawberries, strawberries, wild grapes, grapes, mountain vines, peaches, plums, blueberries, and raspberries belonging to fruits. seeds or two or more, and one or two or more selected from citrus navel, hassaku, unshu mandarin, summer mandarin, orange, iyokan, kumquat, yuzu, kabosu, pomelo, ponkan, lemon, and lime and one or more selected from root crops such as burdock, carrot, garlic, lotus root, and lily root, and grains such as brown rice, glutinous rice, white rice, millet, corn, wheat, barley, millet, and millet. 1 or 2 or more selected from beans and sesame, 1 or 2 or more selected from soybeans, black beans, black sesame, white sesame, adzuki beans, and walnuts belonging to beans and sesame, and kelp belonging to seaweed, One or two or more selected from wakame seaweed, hijiki, seaweed, and kawanori, one or two or more selected from sugars such as brown sugar, fructose, and glucose, and honey, starch, cucumber, and shiso , obtained by fermenting and maturing one or more selected from celery, consisting of the following components and amino acid composition,
For the main ingredient, per 100g, it contains:
Moisture content: 5.0 g to 50.0 g,
Protein: 0.5g-10.0g,
Lipid: 0.05g to 10.00g,
Carbohydrates (sugars): 30.0 g to 75.0 g,
Carbohydrates (fiber): 0.1 g to 5.0 g,
Ash content: 0.5g to 5.0g,
β-carotene: 10 μg to 150 μg,
Vitamin A potency: 10 IU to 100 IU,
Vitamin B 1 : 0.01 mg to 0.50 mg,
Vitamin B2 : 0.01 mg to 0.50 mg,
Vitamin B6 : 0.01 mg to 0.50 mg,
Vitamin E: 10.0 mg or less,
Niacin: 0.1 mg to 6.0 mg,
calcium: 50 mg to 900 mg,
Phosphorus: 200 mg or less,
Iron: 1.0 mg to 5.0 mg,
sodium: 20 mg to 300 mg,
Potassium: 300mg to 1000mg,
magnesium: 40 mg to 200 mg,
Salt equivalent: 0.05 g to 1.00 g,
Copper: 7.0 ppm or less.
For amino acid composition, in 100 g,
isoleucine: 30-200 mg,
Leucine: 50-400 mg,
Lysine: 20-200 mg,
methionine: 10-150 mg,
Cystine: 10-100 mg,
Phenylalanine: 30-250 mg,
Tyrosine: 20-200 mg,
Threonine: 40-200 mg,
tryptophan: 1-100 mg,
Valine: 30-300 mg,
Histidine: 10-200 mg,
Arginine: 40-400 mg,
Alanine: 50-300 mg,
Aspartic acid: 100-600 mg,
Glutamic acid: 100-1200 mg,
Glycine: 30-300 mg,
proline: 40-400 mg,
Serine: 30-300 mg.
The object is to provide a fermented plant composition for feed (for livestock) containing a fermented composition as a main raw material. The fermented composition may be characterized by increasing specific short chain fatty acids in the gut of livestock.
　前記発酵組成物の原材料に、桑、生姜、枇杷のうち１又は２以上の種類を加えた発酵、熟成させることで得られ、前記の成分及びアミノ酸組成からなる発酵組成物を主原料とする飼料用（家畜用）の植物発酵組成材を提供するものである。 A feed mainly composed of a fermented composition composed of the above components and an amino acid composition, obtained by fermentation and aging by adding one or more kinds of mulberry, ginger, and loquat to the raw material of the fermented composition. To provide a fermented plant composition for use (for livestock).
　前記発酵組成物を主原料とする家畜の下痢を抑制させることを特徴とする請求項１又は２に記載のプレバイオテックス食品を含む抗生物質代替材を提供するものである。 The present invention provides an antibiotic substitute containing the prebiotic food according to claim 1 or 2, which is characterized by suppressing diarrhea in domestic animals, which uses the fermented composition as a main raw material.
　果実類に属するリンゴ、柿、バナナ、パインアップル、アケビ、マタタビ、イチジク、野いちご、いちご、山ぶどう、ぶどう、山挑、もも、梅、ブルーベリー、ラズベリー、枇杷から選ばれる１種または２種以上のものと、かんきつ類に属するネーブル、ハッサク、温州みかん、夏みかん、オレンジ、伊予柑、きんかん、ゆず、カボス、ザボン、ポンカン、レモン、ライムから選ばれる１種または２種以上のものと、根菜類に属するゴボウ、ニンジン、ニンニク、レンコン、ゆり根から選ばれる１種または２種以上のものと、穀類に属する玄米、もち米、白米、きび、とうもろこし、小麦、大麦、あわ、ひえから選ばれる１種または２種以上のものと、豆・ゴマ類に属する大豆、黒豆、黒ゴマ、白ゴマ、あずき、くるみから選ばれる１種または２種以上のものと、海草類に属するコンブ、ワカメ、ヒジキ、あおのり、かわのりから選ばれる１種または２種以上のものと、糖類に属する黒糖、果糖、ぶどう糖から選ばれる１種または２種以上のものと、はちみつ、澱粉、きゅうり、しそ、セロリ、桑、生姜から選ばれる１種または２種以上のものとを、発酵、熟成させることで得られ、次の成分及びアミノ酸組成からなる、
主成分について、１００ｇ当たり、下記を含む、
　水分：５．０ｇ～５０．０g、
　タンパク質：０．５ｇ～１０．０ｇ、
　脂質：０．０５ｇ～１０．００ｇ、
　炭水化物（糖質）：３０．０ｇ～７５．０ｇ、
　炭水化物（繊維）：０．１ｇ～５．０ｇ、
　灰分：０．５ｇ～５．０g、
　β－カロチン：１０μｇ～１５０μｇ、
　ビタミンＡ効力：１０ＩＵ～１００ＩＵ、
　ビタミンＢ1：０．０１ｍｇ～０．５０ｍｇ、
　ビタミンＢ2：０．０１ｍｇ～０．５０ｍｇ、
　ビタミンＢ6：０．０１ｍｇ～０．５０ｍｇ、
　ビタミンＥ：１０．０ｍｇ以下、
　ナイアシン：０．１ｍｇ～６．０ｍｇ、
　カルシウム：５０ｍｇ～９００ｍｇ、
　リン：２００ｍｇ以下、
　鉄：１．０ｍｇ～５．０ｍｇ、
　ナトリウム：２０ｍｇ～３００ｍｇ、
　カリウム：３００ｍｇ～１０００ｍｇ、
　マグネシウム：４０ｍｇ～２００ｍｇ、
　食塩相当量：０．０５ｇ～１．００ｇ、
　銅：７．０ｐｐｍ以下。
アミノ酸組成について、１００ｇ中、
　イソロイシン：３０～２００ｍｇ、
　ロイシン：５０～４００ｍｇ、
　リジン：２０～２００ｍｇ、
　メチオニン：１０～１５０ｍｇ、
　シスチン：１０～１００ｍｇ、
　フェニルアラニン：３０～２５０ｍｇ、
　チロシン：２０～２００ｍｇ、
　スレオニン：４０～２００ｍｇ、
　トリプトファン：１～１００ｍｇ、
　バリン：３０～３００ｍｇ、
　ヒスチジン：１０～２００ｍｇ、
　アルギニン：４０～４００ｍｇ、
　アラニン：５０～３００ｍｇ、
　アスパラキン酸：１００～６００ｍｇ、
　グルタミン酸：１００～１２００ｍｇ、
　グリシン：３０～３００ｍｇ、
　プロリン：４０～４００ｍｇ、
　セリン：３０～３００ｍｇ。
発酵組成物を主原料とする飼料用のプレバイオテックス添加物を家畜に給餌することによる家畜の飼育方法としても良い。この発酵組成物は、家畜の腸内で特定の短鎖脂肪酸を増加させる特徴のあるものとしても良い。 One or two fruits selected from apples, persimmons, bananas, pineapples, akebi, actinidia, figs, wild strawberries, strawberries, wild grapes, grapes, wild grapes, peaches, plums, blueberries, raspberries, and loquats. 1 or 2 or more selected from citrus navel, hassaku, unshu mandarin, summer mandarin, orange, iyokan, kumquat, yuzu, kabosu, pomelo, ponkan, lemon, lime, and root vegetables one or more selected from burdock, carrot, garlic, lotus root, and lily root belonging to , and one selected from grains including brown rice, glutinous rice, white rice, millet, corn, wheat, barley, millet, and millet Seeds or two or more; one or two or more selected from soybeans, black soybeans, black sesame, white sesame, adzuki beans, and walnuts belonging to beans and sesame; One or two or more selected from green laver and kawanori, one or two or more selected from brown sugar, fructose, and glucose belonging to sugars, honey, starch, cucumber, perilla, celery, and mulberry , obtained by fermenting and aging one or more selected from ginger, consisting of the following ingredients and amino acid composition,
For the main ingredient, per 100g, it contains:
Moisture content: 5.0 g to 50.0 g,
Protein: 0.5g-10.0g,
Lipid: 0.05g to 10.00g,
Carbohydrates (sugars): 30.0 g to 75.0 g,
Carbohydrates (fiber): 0.1 g to 5.0 g,
Ash content: 0.5g to 5.0g,
β-carotene: 10 μg to 150 μg,
Vitamin A potency: 10 IU to 100 IU,
Vitamin B 1 : 0.01 mg to 0.50 mg,
Vitamin B2 : 0.01 mg to 0.50 mg,
Vitamin B6 : 0.01 mg to 0.50 mg,
Vitamin E: 10.0 mg or less,
Niacin: 0.1 mg to 6.0 mg,
calcium: 50 mg to 900 mg,
Phosphorus: 200 mg or less,
Iron: 1.0 mg to 5.0 mg,
sodium: 20 mg to 300 mg,
Potassium: 300mg to 1000mg,
magnesium: 40 mg to 200 mg,
Salt equivalent: 0.05 g to 1.00 g,
Copper: 7.0 ppm or less.
For amino acid composition, in 100 g,
isoleucine: 30-200 mg,
Leucine: 50-400 mg,
Lysine: 20-200 mg,
methionine: 10-150 mg,
Cystine: 10-100 mg,
Phenylalanine: 30-250 mg,
Tyrosine: 20-200 mg,
Threonine: 40-200 mg,
tryptophan: 1-100 mg,
Valine: 30-300 mg,
Histidine: 10-200 mg,
Arginine: 40-400 mg,
Alanine: 50-300 mg,
Aspartic acid: 100-600 mg,
Glutamic acid: 100-1200 mg,
Glycine: 30-300 mg,
proline: 40-400 mg,
Serine: 30-300 mg.
A method of raising livestock by feeding livestock a prebiotic additive for feed containing the fermented composition as a main raw material may also be used. The fermented composition may be characterized by increasing specific short chain fatty acids in the gut of livestock.
　前記発酵組成物を主原料とする飼料用のプレバイオテックス添加物を家畜に給餌することによる抗生物質の非投与若しくは使用量を削減するとする家畜の飼育方法としても良い。この発酵組成物は、家畜の腸内で特定の短鎖脂肪酸を増加させる特徴のあるものとしても良い。 A livestock breeding method may be adopted in which antibiotics are not administered or the amount used is reduced by feeding livestock with a prebiotic additive for feed, the main raw material of which is the fermented composition. The fermented composition may be characterized by increasing specific short chain fatty acids in the gut of livestock.
　本件発酵組成物を健常な家畜に与えることによって、抗生物質を非投与とした家畜の飼育条件下において、抗生物質の投与群と同程度の家畜の生育状況とすることができた。 By giving the fermented composition of this invention to healthy livestock, it was possible to achieve the same level of growth of livestock as the antibiotic-administered group under livestock breeding conditions in which antibiotics were not administered.
　具体的には、本件発酵組成物の投与群は、抗生物質の投与群と比較して同程度の家畜の増体を維持して、血液、腸管の長さや重量、絨毛組織に有意な変化と異常所見を与えず、下痢症状（糞便スコア）も同程度であった。 Specifically, the administration group of the present fermented composition maintained the same level of weight gain of livestock as compared to the antibiotic administration group, and the blood, the length and weight of the intestinal tract, and the villous tissue showed significant changes. No abnormal findings were observed, and diarrhea symptoms (stool score) were similar.
　以下、添付図面及び実施例を組み合わせて本発明を更に説明する。 The present invention will be further described below in combination with the accompanying drawings and examples.
食用豚の成長成績を示すグラフGraph showing the growth performance of edible pigs 食用豚の腸内環境（糞便スコア、糞便中IgA濃度）を評価するグラフGraph to evaluate the intestinal environment of edible pigs (fecal score, fecal IgA concentration) 食用豚の腸内短鎖脂肪酸濃度を示すグラフGraph showing intestinal short-chain fatty acid concentrations in edible pigs 食用豚の消化管形状及び血液生化学検査を示す表Table showing gastrointestinal morphology and blood biochemistry of edible pigs 本件発酵組成物の食用豚に与える効果機序の説明図Explanatory diagram of the effect mechanism of the present fermented composition on edible pigs
　本発明者は、本発酵組成物に着目をして、抗生物質を非投与とした家畜条件下での家畜（特に、食用豚）の育成において、その生育に影響を与えるか否かを実験した。 The present inventor focused on the present fermented composition and conducted an experiment to determine whether or not it affects the growth of livestock (especially edible pigs) under livestock conditions in which antibiotics are not administered. .
　本発酵組成物（FBP）は、果実類に属するリンゴ、柿、バナナ、パインアップル、アケビ、マタタビ、イチジク、野いちご、いちご、山ぶどう、ぶどう、山挑、もも、梅、ブルーベリー、ラズベリー、枇杷から選ばれる１種または２種以上のものと、かんきつ類に属するネーブル、ハッサク、温州みかん、夏みかん、オレンジ、伊予柑、きんかん、ゆず、カボス、ザボン、ポンカン、レモン、ライムから選ばれる１種または２種以上のものと、根菜類に属するゴボウ、ニンジン、ニンニク、レンコン、ゆり根から選ばれる１種または２種以上のものと、穀類に属する玄米、もち米、白米、きび、とうもろこし、小麦、大麦、あわ、ひえから選ばれる１種または２種以上のものと、豆・ゴマ類に属する大豆、黒豆、黒ゴマ、白ゴマ、あずき、くるみから選ばれる１種または２種以上のものと、海草類に属するコンブ、ワカメ、ヒジキ、あおのり、かわのりから選ばれる１種または２種以上のものと、糖類に属する黒糖、果糖、ぶどう糖から選ばれる１種または２種以上のものと、はちみつ、澱粉、きゅうり、しそ、セロリ、桑、生姜から選ばれる１種または２種以上のものとを、発酵、熟成させることで得られるものである。発酵熟成期間は、3年3か月以上とした。また、発酵熟成過程においては、添加物、熱、水を人為的に加えず、且つ、３年以上静置での発酵とした。本実験では、各類から１種又は２種を選択して、15種類の原材料を選択して発酵させたもの（本発酵組成物）を用いた。
  This fermented composition (FBP) contains apples, persimmons, bananas, pineapples, akebi, actinidia, figs, wild strawberries, strawberries, wild grapes, grapes, wild grapes, peaches, plums, blueberries, raspberries, One or two or more kinds selected from loquat, and one or more kinds selected from citrus navel, hassaku, unshu orange, summer orange, orange, iyokan, kumquat, yuzu, kabosu, pomelo, ponkan, lemon, and lime Two or more kinds, one or more kinds selected from burdock, carrot, garlic, lotus root, and lily bulb belonging to root crops, and brown rice, glutinous rice, white rice, millet, corn, wheat, 1 or 2 or more selected from barley, foxtail millet, and millet; One or two or more selected from kelp, wakame, hijiki, green laver, and kawanori belonging to seaweeds, one or two or more selected from brown sugar, fructose, and glucose belonging to sugars, honey, It is obtained by fermenting and maturing one or more selected from starch, cucumber, perilla, celery, mulberry and ginger. The fermentation and maturation period was 3 years and 3 months or longer. In addition, in the fermentation and aging process, no additives, heat, or water were artificially added, and the fermentation was carried out in a stationary state for three years or more. In this experiment, 1 or 2 types were selected from each type, and 15 types of raw materials were selected and fermented (this fermented composition) was used.
　本実験で用いた導入豚は、23日齢ほ乳期子豚(交雑種)とした。試験設計は、抗生物質を含む一般的な飼料(対照区)および抗生物質をFBP（本発酵組成物）に置き換えた飼料(試験区)で飼育して比較した。対象区及び試験区ともに、同じ子豚用配合飼料を用いた。対象区には抗生物質としてアビラマイシンを４０ｐｐｍ加えて、試験区には抗生物質を添加しないものとした。反対に、試験区には本発酵組成物として０．１２５％を加えて、対照区には本発酵組成物を添加しないものとした。 The introduced pigs used in this experiment were 23-day-old suckling piglets (crossbreed). In the test design, rats were fed with a general feed containing antibiotics (control group) and a feed (test group) in which the antibiotic was replaced with FBP (this fermented composition) for comparison. The same mixed feed for piglets was used in both the control group and the test group. 40 ppm of aviramycin was added as an antibiotic to the control group, and no antibiotic was added to the test group. On the contrary, 0.125% of the present fermented composition was added to the test plot, and the present fermented composition was not added to the control plot.
　本実験での摂食期間は、23日齢から50日齢とした。飼育環境は、単飼ケージとして、給餌方法は、自由飲水、及び不断給餌とした。子豚用配合飼料は、飼養段階に合わせた配合飼料を使用した。アビラマイシン（抗生物質）は、対照区では４０ｐｐｍ、試験区では０ｐｐｍとした。他方で、FBP（本発酵組成物）は、対照区では０％、試験区では０．１２５％とした。 The feeding period in this experiment was from 23 days old to 50 days old. The breeding environment was a single cage, and the feeding method was free drinking water and constant feeding. The mixed feed for piglets used a mixed feed suitable for the breeding stage. Avilamycin (antibiotic) was 40 ppm in the control group and 0 ppm in the test group. On the other hand, FBP (this fermented composition) was 0% in the control group and 0.125% in the test group.
　採材部位及び測定項目の組み合わせは、「ふん、糞便スコア・IgA」、「経静脈血、血液生化学分析」、「盲腸静脈血、短鎖脂肪酸分析」、「盲腸内容部物、短鎖脂肪酸分析」、「小腸・大腸、臓器測定・絨毛長・陰窩深さ」とした。 The combination of sampling sites and measurement items is "feces, feces score/IgA", "intravenous blood, blood biochemical analysis", "cecal venous blood, short chain fatty acid analysis", "cecal contents, short chain fatty acid analysis”, and “small intestine/large intestine, organ measurement/villus length/crypt depth”.
　次に、糞便スコアを説明する。良好な便をコロコロ便としてスコア０とした。ソフトな便をスコア１として、下痢状の便をスコア２、水溶性の便をスコア３，より水溶性の程度の高い下痢をスコア４とした。スコアの数値が高くなると不良な便となる。 Next, I will explain the fecal score. A score of 0 was assigned to a good stool, which was regarded as a stool. Soft stool was scored as 1, diarrheal stool as score 2, watery stool as score 3, and more water-soluble diarrhea as score 4. The higher the score, the worse the stool.
　実験結果について説明する。図１（左）は、食用豚の成長成績を示すグラフである。まず、抗生物質を含む一般的な飼料(対照区)および抗生物質をFBP（本発酵組成物）に置き換えた飼料(試験区)で飼育した食用豚について、体重を測定してその推移をグラフにした。縦軸は重量（ｋｇ）、横軸は生後日齢（日）である。対照区及び試験区ともに、時間とともに同程度の体重増加が見られた。また、飼料要求率は、〔飼料摂取量（または消費量）（kg）／畜産物の生産量（kg）〕で算出され、図１（右）のグラフの縦軸は、kg/kgのため飼料要求率は無単位数である。 Explain the results of the experiment. FIG. 1 (left) is a graph showing the growth performance of edible pigs. First, the body weight of edible pigs bred with a general feed containing antibiotics (control group) and a feed in which the antibiotic was replaced with FBP (this fermented composition) (test group) was measured and graphed. bottom. The vertical axis is weight (kg), and the horizontal axis is age after birth (days). Both the control group and the test group showed similar weight gain over time. In addition, the feed conversion rate is calculated as [feed intake (or consumption) (kg) / livestock product production (kg)]. The feed conversion rate is a unitless number.
　次に、対照区及び試験区において、特定の時期における食用豚の飼料要求率を測定した（図１右）。生後日齢を重ねるごとに飼料要求率は高くなり、37日－44日の生後日齢でピークとなり、44日－55日の生後日齢で若干、飼料要求率は低くなる傾向にある。対照区及び試験区においても、飼料要求率はほぼ同じ数値を示した。なお、飼料要求率は、畜産物1kg当たりの生産に要する摂取（または消費）飼料数量のことで、増体など生産のために何倍の飼料を必要とするかを示し、飼料要求率が小さいほど効率が良いことを示すものである。 Next, we measured the feed conversion rate of edible pigs at a specific time in the control and test plots (Fig. 1 right). The feed conversion rate increases as the postnatal age increases, reaching a peak at the 37th to 44th day postnatal age, and tends to decrease slightly at the 44th to 55th postnatal age. The feed conversion rate showed almost the same values in the control plot and the test plot. The feed conversion rate is the amount of feed consumed (or consumed) required for production of 1 kg of livestock product. This indicates that the higher the efficiency, the better.
　図２は、対照区及び試験区において、特定の時期における腸内環境を観察したデータを示す。左グラフは、特定の日齢期間での糞便スコアを示す。糞便スコアの評価方法は、上述の通りである。対照区及び試験区ともに、糞便スコアは良好となり、期間中の腸内環境は安定していた。右グラフは、特定の日齢（23日、31日、50日）の糞便IgAスコアを示す。安静時における腸管免疫の変化は見られなかった。なお、糞便IgA濃度は腸管内の免疫状態と相関する。感染症などで糞便IgA濃度は高値を示し、逆に糞便IgA濃度が極端に低下すれば感染症リスクが高くなる。ここでは両群ともに低値で安定していたことから、腸内環境が比較的安定していたことを示すものと考えられる。 Figure 2 shows data obtained by observing the intestinal environment at specific times in the control and test plots. The left graph shows fecal scores at specific age periods. The fecal score evaluation method is as described above. The fecal score was good in both the control group and the test group, and the intestinal environment was stable during the period. Right graph shows fecal IgA scores at specific ages (23 days, 31 days, 50 days). No change in resting intestinal immunity was observed. In addition, the fecal IgA concentration correlates with the immune status in the intestinal tract. The fecal IgA concentration is high due to infectious diseases, and conversely, if the fecal IgA concentration is extremely low, the risk of infectious diseases increases. Since the values were low and stable in both groups, it is thought that this indicates that the intestinal environment was relatively stable.
　図３は、対照区及び試験区において、盲腸内容物及び盲腸静脈血での短鎖脂肪酸濃度を示したグラフである。短鎖脂肪酸は、酢酸、プロピオン酸、ｎ酪酸を測定した。盲腸内容物、静脈血ともに、対照区及び試験区の両群において、FBP給与で全体的な短鎖脂肪酸の構成比に大きな変動は見られなかったが、試験区においては短鎖脂肪酸濃度が高くなる傾向が認められた。 Fig. 3 is a graph showing short-chain fatty acid concentrations in cecal contents and cecal venous blood in the control and test groups. Acetic acid, propionic acid, and n-butyric acid were measured as short-chain fatty acids. Both the cecal contents and the venous blood showed no significant change in the composition ratio of overall short-chain fatty acids with FBP feeding in both the control group and the test group, but the short-chain fatty acid concentration was high in the test group. A trend was observed.
　これらの短鎖脂肪酸を測定したのは、短鎖脂肪酸の有益性が注目されているからである。腸の粘膜は病原体の侵入を防ぐという腸管バリア機能を担っている。酪酸やプロピオン酸には、腸粘膜を維持して、腸のバリア機能を高める働きがある。特に、酪酸は腸上皮細胞の最も重要なエネルギー源であり、腸管上皮の新陳代謝を促進し、また、腸管の蠕動運動を促進すると報告されている。 These short-chain fatty acids were measured because the benefits of short-chain fatty acids are attracting attention. The intestinal mucosa plays an intestinal barrier function to prevent invasion of pathogens. Butyric acid and propionic acid have the function of maintaining the intestinal mucosa and enhancing the intestinal barrier function. In particular, butyric acid is the most important energy source for intestinal epithelial cells, and has been reported to promote the metabolism of the intestinal epithelium and the peristaltic movement of the intestinal tract.
　図４は、食用豚の消化管形状及び血液生化学検査を示す表である。対照区及び試験区において、小腸（長さ、重量）、大腸（長さ、重量）、消化吸収（絨毛高さ(μm)、陰窩深さ(μm) 、絨毛／陰窩深さ）といった解剖学的データ（消化管形状）については、両群において概ね差異がなかった。 Fig. 4 is a table showing the shape of the gastrointestinal tract and blood biochemical examination of edible pigs. Anatomy such as small intestine (length, weight), large intestine (length, weight), digestive absorption (villus height (μm), crypt depth (μm), villus/crypt depth) in control and test groups There was almost no difference between the two groups in terms of clinical data (gastrointestinal tract shape).
　また、血液生化学検査でも、対照区及び試験区において、γ-GT、アルブミン、総コレステロール、尿素窒については、両群において概ね差異がなかった。これらの知見は、食用豚の成長成績を示すグラフ（図１）、食用豚の腸内環境（糞便スコア、糞便中IgA濃度）を評価するグラフ（図２）の結果と矛盾することなく、補強するものである。 Also, in the blood biochemical tests, there was almost no difference between the control group and the test group in terms of γ-GT, albumin, total cholesterol, and urea nitrogen. These findings are consistent with the results of the graph showing the growth performance of edible pigs (Fig. 1) and the graph (Fig. 2) evaluating the intestinal environment (fecal score, fecal IgA concentration) of edible pigs. It is something to do.
　図５は、本件発酵組成物の食用豚に与える効果機序の説明図である。食用豚の飼育において、生体に対するストレスや疾病によって、消化管内にいわゆる有害菌が増殖して、これらの有害菌が毒素や腐敗物を産出させて、消化管内部に損傷を与えることになる。具体的には、消化吸収機能を担う絨毛、陰窩の損傷を与えて結果として、下痢を引き起こすものとなる。 Fig. 5 is an explanatory diagram of the effect mechanism of the present fermented composition on edible pigs. In the rearing of edible pigs, so-called harmful bacteria proliferate in the digestive tract due to stress or disease on the living body, and these harmful bacteria produce toxins and putrefactive substances that damage the inside of the digestive tract. Specifically, it damages the villi and crypts responsible for digestive and absorption functions, resulting in diarrhea.
　従来は、上記の対策として、家畜の飼育時に抗生物質を与えることで、消化管内のいわゆる有害菌を除去して、下痢などを防止して生育を図った。しかし、有害菌の中には、抗生物質に抵抗性のある菌が出現してしまうものがあった。 In the past, as a countermeasure to the above, antibiotics were given to livestock during breeding to remove the so-called harmful bacteria in the digestive tract, prevent diarrhea, etc., and promote growth. However, among the harmful bacteria, there were some bacteria that were resistant to antibiotics.
　本発明に係る本件発酵組成物は、抗生物質を与えない場合も同様の効果が得られることから、家畜の飼育に用いられる抗生物質代替材とすることができた。すなわち、本件発酵組成物は、家畜の消化管内の有用菌を増加させて、この有用菌からの短鎖脂肪酸の生産を促し、栄養素利用推進され、良好な腸内環境を図ることができると考える。これは、抗生物質を与えない場合も同様の効果が得られることから、本発明に係る本件発酵組成物は、家畜の飼育に用いられる抗生物質代替材とすることができた。 The fermented composition according to the present invention could be used as an antibiotic substitute material for raising livestock because the same effect can be obtained even when antibiotics are not given. That is, it is believed that the fermented composition increases useful bacteria in the gastrointestinal tract of livestock, promotes the production of short-chain fatty acids from these useful bacteria, promotes the utilization of nutrients, and can achieve a favorable intestinal environment. . Since the same effect can be obtained even when antibiotics are not given, the fermented composition according to the present invention was able to be used as an antibiotic substitute material for raising livestock.

Claims (5)

  1. 果実類に属するリンゴ、柿、バナナ、パインアップル、アケビ、マタタビ、イチジク、野いちご、いちご、山ぶどう、ぶどう、山挑、もも、梅、ブルーベリー、ラズベリーから選ばれる１種または２種以上のものと、かんきつ類に属するネーブル、ハッサク、温州みかん、夏みかん、オレンジ、伊予柑、きんかん、ゆず、カボス、ザボン、ポンカン、レモン、ライムから選ばれる１種または２種以上のものと、根菜類に属するゴボウ、ニンジン、ニンニク、レンコン、ゆり根から選ばれる１種または２種以上のものと、穀類に属する玄米、もち米、白米、きび、とうもろこし、小麦、大麦、あわ、ひえから選ばれる１種または２種以上のものと、豆・ゴマ類に属する大豆、黒豆、黒ゴマ、白ゴマ、あずき、くるみから選ばれる１種または２種以上のものと、海草類に属するコンブ、ワカメ、ヒジキ、あおのり、かわのりから選ばれる１種または２種以上のものと、糖類に属する黒糖、果糖、ぶどう糖から選ばれる１種または２種以上のものと、はちみつ、澱粉、きゅうり、しそ、セロリから選ばれる１種または２種以上のものとを、発酵、熟成させることで得られ、次の成分及びアミノ酸組成からなる、
    主成分について、１００ｇ当たり、下記を含む、
    　水分：５．０ｇ～５０．０g、
    　タンパク質：０．５ｇ～１０．０ｇ、
    　脂質：０．０５ｇ～１０．００ｇ、
    　炭水化物（糖質）：３０．０ｇ～７５．０ｇ、
    　炭水化物（繊維）：０．１ｇ～５．０ｇ、
    　灰分：０．５ｇ～５．０g、
    　β－カロチン：１０μｇ～１５０μｇ、
    　ビタミンＡ効力：１０ＩＵ～１００ＩＵ、
    　ビタミンＢ1：０．０１ｍｇ～０．５０ｍｇ、
    　ビタミンＢ2：０．０１ｍｇ～０．５０ｍｇ、
    　ビタミンＢ6：０．０１ｍｇ～０．５０ｍｇ、
    　ビタミンＥ：１０．０ｍｇ以下、
    　ナイアシン：０．１ｍｇ～６．０ｍｇ、
    　カルシウム：５０ｍｇ～９００ｍｇ、
    　リン：２００ｍｇ以下、
    　鉄：１．０ｍｇ～５．０ｍｇ、
    　ナトリウム：２０ｍｇ～３００ｍｇ、
    　カリウム：３００ｍｇ～１０００ｍｇ、
    　マグネシウム：４０ｍｇ～２００ｍｇ、
    　食塩相当量：０．０５ｇ～１．００ｇ、
    　銅：７．０ｐｐｍ以下。
    アミノ酸組成について、１００ｇ中、
    　イソロイシン：３０～２００ｍｇ、
    　ロイシン：５０～４００ｍｇ、
    　リジン：２０～２００ｍｇ、
    　メチオニン：１０～１５０ｍｇ、
    　シスチン：１０～１００ｍｇ、
    　フェニルアラニン：３０～２５０ｍｇ、
    　チロシン：２０～２００ｍｇ、
    　スレオニン：４０～２００ｍｇ、
    　トリプトファン：１～１００ｍｇ、
    　バリン：３０～３００ｍｇ、
    　ヒスチジン：１０～２００ｍｇ、
    　アルギニン：４０～４００ｍｇ、
    　アラニン：５０～３００ｍｇ、
    　アスパラキン酸：１００～６００ｍｇ、
    　グルタミン酸：１００～１２００ｍｇ、
    　グリシン：３０～３００ｍｇ、
    　プロリン：４０～４００ｍｇ、
    　セリン：３０～３００ｍｇ。
    発酵組成物を主原料とする腸内で特定の短鎖脂肪酸を増加させる特徴のある飼料用（家畜用）の植物発酵組成材。     One or two or more kinds selected from apples, persimmons, bananas, pineapples, akebi, Actinidia, figs, wild strawberries, strawberries, wild grapes, grapes, wild grapes, peaches, plums, blueberries, and raspberries belonging to fruits Citrus fruits, one or more kinds selected from navel, hassaku, unshu mandarin, summer mandarin orange, orange, Iyokan, kumquat, yuzu, kabosu, pomelo, ponkan, lemon, lime, and root vegetables One or two or more selected from burdock, carrot, garlic, lotus root, and lily root, and one or more selected from grains such as brown rice, glutinous rice, white rice, millet, corn, wheat, barley, millet, and millet Two or more kinds, one or more kinds selected from soybeans, black soybeans, black sesame seeds, white sesame seeds, adzuki beans, and walnuts belonging to the beans and sesame family, and kelp, wakame seaweed, hijiki seaweed, and green laver belonging to the seaweed family , one or two or more selected from kawanori, one or two or more selected from brown sugar, fructose, and glucose belonging to sugars, and honey, starch, cucumber, perilla, and celery Obtained by fermenting and maturing seeds or two or more, consisting of the following ingredients and amino acid composition:
    For the main ingredient, per 100g, it contains:
    Moisture content: 5.0 g to 50.0 g,
    Protein: 0.5g-10.0g,
    Lipid: 0.05g to 10.00g,
    Carbohydrates (sugars): 30.0 g to 75.0 g,
    Carbohydrates (fiber): 0.1 g to 5.0 g,
    Ash content: 0.5g to 5.0g,
    β-carotene: 10 μg to 150 μg,
    Vitamin A potency: 10 IU to 100 IU,
    Vitamin B1: 0.01 mg to 0.50 mg,
    Vitamin B2: 0.01 mg to 0.50 mg,
    Vitamin B6: 0.01 mg to 0.50 mg,
    Vitamin E: 10.0 mg or less,
    Niacin: 0.1 mg to 6.0 mg,
    calcium: 50 mg to 900 mg,
    Phosphorus: 200 mg or less,
    Iron: 1.0 mg to 5.0 mg,
    sodium: 20 mg to 300 mg,
    Potassium: 300mg to 1000mg,
    magnesium: 40 mg to 200 mg,
    Salt equivalent: 0.05 g to 1.00 g,
    Copper: 7.0 ppm or less.
    For amino acid composition, in 100 g,
    isoleucine: 30-200 mg,
    Leucine: 50-400 mg,
    Lysine: 20-200 mg,
    methionine: 10-150 mg,
    Cystine: 10-100 mg,
    Phenylalanine: 30-250 mg,
    Tyrosine: 20-200 mg,
    Threonine: 40-200 mg,
    tryptophan: 1-100 mg,
    Valine: 30-300 mg,
    Histidine: 10-200 mg,
    Arginine: 40-400 mg,
    Alanine: 50-300 mg,
    Aspartic acid: 100-600 mg,
    Glutamic acid: 100-1200 mg,
    Glycine: 30-300 mg,
    proline: 40-400 mg,
    Serine: 30-300 mg.
    A plant fermented composition for feed (for livestock) characterized by increasing specific short-chain fatty acids in the intestine, which is mainly composed of a fermented composition.
  2. 前記発酵組成物の原材料に、桑、生姜、枇杷のうち１又は２以上の種類を加えて、発酵、熟成させることで得られ、前記の成分及びアミノ酸組成からなる発酵組成物を主原料とする飼料用（家畜用）の植物発酵組成材。 A fermented composition obtained by adding one or more kinds of mulberry, ginger, and loquat to the raw materials of the fermented composition, fermenting and maturing, and having the above ingredients and amino acid composition as the main raw material. Plant fermented composition material for feed (for livestock).
  3. 前記の発酵組成物を主原料とする家畜の下痢を抑制させることを特徴とする請求項１又は２に記載の抗生物質代替材。 3. The alternative to antibiotics according to claim 1, wherein the fermented composition is used as a main raw material to suppress diarrhea in domestic animals.
  4. 果実類に属するリンゴ、柿、バナナ、パインアップル、アケビ、マタタビ、イチジク、野いちご、いちご、山ぶどう、ぶどう、山挑、もも、梅、ブルーベリー、ラズベリー、枇杷から選ばれる１種または２種以上のものと、かんきつ類に属するネーブル、ハッサク、温州みかん、夏みかん、オレンジ、伊予柑、きんかん、ゆず、カボス、ザボン、ポンカン、レモン、ライムから選ばれる１種または２種以上のものと、根菜類に属するゴボウ、ニンジン、ニンニク、レンコン、ゆり根から選ばれる１種または２種以上のものと、穀類に属する玄米、もち米、白米、きび、とうもろこし、小麦、大麦、あわ、ひえから選ばれる１種または２種以上のものと、豆・ゴマ類に属する大豆、黒豆、黒ゴマ、白ゴマ、あずき、くるみから選ばれる１種または２種以上のものと、海草類に属するコンブ、ワカメ、ヒジキ、あおのり、かわのりから選ばれる１種または２種以上のものと、糖類に属する黒糖、果糖、ぶどう糖から選ばれる１種または２種以上のものと、はちみつ、澱粉、きゅうり、しそ、セロリ、桑、生姜から選ばれる１種または２種以上のものとを、発酵、熟成させることで得られ、次の成分及びアミノ酸組成からなる、
    主成分について、１００ｇ当たり、下記を含む、
    　水分：５．０ｇ～５０．０g、
    　タンパク質：０．５ｇ～１０．０ｇ、
    　脂質：０．０５ｇ～１０．００ｇ、
    　炭水化物（糖質）：３０．０ｇ～７５．０ｇ、
    　炭水化物（繊維）：０．１ｇ～５．０ｇ、
    　灰分：０．５ｇ～５．０g、
    　β－カロチン：１０μｇ～１５０μｇ、
    　ビタミンＡ効力：１０ＩＵ～１００ＩＵ、
    　ビタミンＢ1：０．０１ｍｇ～０．５０ｍｇ、
    　ビタミンＢ2：０．０１ｍｇ～０．５０ｍｇ、
    　ビタミンＢ6：０．０１ｍｇ～０．５０ｍｇ、
    　ビタミンＥ：１０．０ｍｇ以下、
    　ナイアシン：０．１ｍｇ～６．０ｍｇ、
    　カルシウム：５０ｍｇ～９００ｍｇ、
    　リン：２００ｍｇ以下、
    　鉄：１．０ｍｇ～５．０ｍｇ、
    　ナトリウム：２０ｍｇ～３００ｍｇ、
    　カリウム：３００ｍｇ～１０００ｍｇ、
    　マグネシウム：４０ｍｇ～２００ｍｇ、
    　食塩相当量：０．０５ｇ～１．００ｇ、
    　銅：７．０ｐｐｍ以下、
    アミノ酸組成について、１００ｇ中、
    　イソロイシン：３０～２００ｍｇ、
    　ロイシン：５０～４００ｍｇ、
    　リジン：２０～２００ｍｇ、
    　メチオニン：１０～１５０ｍｇ、
    　シスチン：１０～１００ｍｇ、
    　フェニルアラニン：３０～２５０ｍｇ、
    　チロシン：２０～２００ｍｇ、
    　スレオニン：４０～２００ｍｇ、
    　トリプトファン：１～１００ｍｇ、
    　バリン：３０～３００ｍｇ、
    　ヒスチジン：１０～２００ｍｇ、
    　アルギニン：４０～４００ｍｇ、
    　アラニン：５０～３００ｍｇ、
    　アスパラキン酸：１００～６００ｍｇ、
    　グルタミン酸：１００～１２００ｍｇ、
    　グリシン：３０～３００ｍｇ、
    　プロリン：４０～４００ｍｇ、
    　セリン：３０～３００ｍｇ。
    発酵組成物を主原料とする腸内で特定の短鎖脂肪酸を増加させる飼料用（家畜用）の植物発酵組成材を家畜に給餌することによる家畜の飼育方法。     One or two fruits selected from apples, persimmons, bananas, pineapples, akebi, actinidia, figs, wild strawberries, strawberries, wild grapes, grapes, wild grapes, peaches, plums, blueberries, raspberries, and loquats. 1 or 2 or more selected from citrus navel, hassaku, unshu mandarin, summer mandarin, orange, iyokan, kumquat, yuzu, kabosu, pomelo, ponkan, lemon, lime, and root vegetables one or more selected from burdock, carrot, garlic, lotus root, and lily root belonging to , and one selected from grains including brown rice, glutinous rice, white rice, millet, corn, wheat, barley, millet, and millet Seeds or two or more; one or two or more selected from soybeans, black soybeans, black sesame, white sesame, adzuki beans, and walnuts belonging to beans and sesame; One or two or more selected from green laver and kawanori, one or two or more selected from brown sugar, fructose, and glucose belonging to sugars, honey, starch, cucumber, perilla, celery, and mulberry , obtained by fermenting and aging one or more selected from ginger, consisting of the following ingredients and amino acid composition,
    For the main ingredient, per 100g, it contains:
    Moisture content: 5.0 g to 50.0 g,
    Protein: 0.5g-10.0g,
    Lipid: 0.05g to 10.00g,
    Carbohydrates (sugars): 30.0 g to 75.0 g,
    Carbohydrates (fiber): 0.1 g to 5.0 g,
    Ash content: 0.5g to 5.0g,
    β-carotene: 10 μg to 150 μg,
    Vitamin A potency: 10 IU to 100 IU,
    Vitamin B 1 : 0.01 mg to 0.50 mg,
    Vitamin B2 : 0.01 mg to 0.50 mg,
    Vitamin B6 : 0.01 mg to 0.50 mg,
    Vitamin E: 10.0 mg or less,
    Niacin: 0.1 mg to 6.0 mg,
    calcium: 50 mg to 900 mg,
    Phosphorus: 200 mg or less,
    Iron: 1.0 mg to 5.0 mg,
    sodium: 20 mg to 300 mg,
    Potassium: 300mg to 1000mg,
    magnesium: 40 mg to 200 mg,
    Salt equivalent: 0.05 g to 1.00 g,
    Copper: 7.0 ppm or less,
    For amino acid composition, in 100 g,
    isoleucine: 30-200 mg,
    Leucine: 50-400 mg,
    Lysine: 20-200 mg,
    methionine: 10-150 mg,
    Cystine: 10-100 mg,
    Phenylalanine: 30-250 mg,
    Tyrosine: 20-200 mg,
    Threonine: 40-200 mg,
    tryptophan: 1-100 mg,
    Valine: 30-300 mg,
    Histidine: 10-200 mg,
    Arginine: 40-400 mg,
    Alanine: 50-300 mg,
    Aspartic acid: 100-600 mg,
    Glutamic acid: 100-1200 mg,
    Glycine: 30-300 mg,
    proline: 40-400 mg,
    Serine: 30-300 mg.
    A method of rearing livestock by feeding livestock a plant fermented composition for feed (for livestock) that increases specific short-chain fatty acids in the intestine, the main raw material being the fermented composition.
  5. 前記発酵組成物を主原料とする腸内で特定の短鎖脂肪酸を増加させる飼料用（家畜用）の植物発酵組成材を家畜に給餌することによる、抗生物質を非投与若しくは使用量を削減する家畜の飼育方法。 By feeding livestock with a plant fermented composition for feed (for livestock) that uses the fermented composition as a main raw material and increases specific short-chain fatty acids in the intestine, antibiotics are not administered or the amount used is reduced. How to raise livestock.
PCT/JP2022/038769 2021-12-24 2022-10-18 Fermented composition for feed, and method for raising livestock WO2023119820A1 (en)

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Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0787899A (en) * 1993-09-22 1995-04-04 Hokueiken Corp:Kk Additive for hog-raising feed
WO1998001042A1 (en) * 1996-07-10 1998-01-15 Manda Fermentation Co., Ltd. Fermentation composition, process for preparing the same, and use thereof
JP2001269125A (en) * 2000-03-29 2001-10-02 Nihon Nosan Kogyo Kk Feed for pig
JP2003238400A (en) * 2002-02-12 2003-08-27 Nippon Zenyaku Kogyo Kk Anticoccidial composition and fodder containing the same
JP2005065671A (en) * 2003-08-26 2005-03-17 Gouda:Kk Fermented feed
JP2007084504A (en) * 2005-09-26 2007-04-05 Sanwa Shiyurui Kk Composition containing fermentation product of barley as active ingredient and having neovascularization inhibitory action
JP2014011994A (en) * 2012-06-07 2014-01-23 Takara Bio Inc Composition containing basidiomycete which is cultured on culture medium containing brewing lees
WO2015197623A1 (en) * 2014-06-26 2015-12-30 Novozymes A/S Improved protein bio-availability of palm kernel cake using milder expeller conditions and enzymatic treatment
JP2019182763A (en) * 2018-04-05 2019-10-24 株式会社ダイワ Composition, and food and feed containing the same
JP2020043838A (en) * 2018-09-21 2020-03-26 天草梅肉ポーク株式会社 Livestock feed additive and method for producing the same

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0787899A (en) * 1993-09-22 1995-04-04 Hokueiken Corp:Kk Additive for hog-raising feed
WO1998001042A1 (en) * 1996-07-10 1998-01-15 Manda Fermentation Co., Ltd. Fermentation composition, process for preparing the same, and use thereof
JP2001269125A (en) * 2000-03-29 2001-10-02 Nihon Nosan Kogyo Kk Feed for pig
JP2003238400A (en) * 2002-02-12 2003-08-27 Nippon Zenyaku Kogyo Kk Anticoccidial composition and fodder containing the same
JP2005065671A (en) * 2003-08-26 2005-03-17 Gouda:Kk Fermented feed
JP2007084504A (en) * 2005-09-26 2007-04-05 Sanwa Shiyurui Kk Composition containing fermentation product of barley as active ingredient and having neovascularization inhibitory action
JP2014011994A (en) * 2012-06-07 2014-01-23 Takara Bio Inc Composition containing basidiomycete which is cultured on culture medium containing brewing lees
WO2015197623A1 (en) * 2014-06-26 2015-12-30 Novozymes A/S Improved protein bio-availability of palm kernel cake using milder expeller conditions and enzymatic treatment
JP2019182763A (en) * 2018-04-05 2019-10-24 株式会社ダイワ Composition, and food and feed containing the same
JP2020043838A (en) * 2018-09-21 2020-03-26 天草梅肉ポーク株式会社 Livestock feed additive and method for producing the same

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