WO2023098604A1

WO2023098604A1 - Pcr plate sealing film having high sealing performance

Info

Publication number: WO2023098604A1
Application number: PCT/CN2022/134589
Authority: WO
Inventors: 刘子德; 刘子贤; 刘刚; 袁义勇; 赵华; 唐波
Original assignee: 美东汇成生命科技(昆山)有限公司
Priority date: 2021-12-02
Filing date: 2022-11-28
Publication date: 2023-06-08
Also published as: CN114292722A; CN114292722B

Abstract

The present application relates to the technical field of experimental equipment, and provides a PCR plate sealing film having high sealing performance, comprising a bearing plate. The lower surface of the bearing plate is provided with a plate sealing film body; the upper surface of the bearing plate is fixedly provided with a mounting housing; the center of the upper surface of the mounting housing is provided with an adjustment mechanism; an inner partition plate is mounted in the mounting housing; a first limiting mechanism is provided in the mounting housing and located above the inner partition plate; and a second limiting mechanism is provided in the mounting housing and located below the inner partition plate. According to the present application, by means of the matching of a first snap-fit block and a slot, a pressing rod pushes a second connection rod to move, such that a limiting baffle can move downwards, the peripheries of the plate sealing film body and a PCR plate can be sealed, the limiting baffle can be better attached to the plate sealing film body, and the sealing effect of the plate sealing film body is better.

Description

A strong sealing PCR sealing film

technical field

The invention relates to the technical field of experimental equipment, in particular to a strong sealing PCR sealing film.

Background technique

The PCR plate is a container used to place some specific experimental reactants. The PCR plate needs to be sealed with a sealing film during transportation and storage. For example, the patent application number 202022266520.0 proposes a seal for PCR reaction plates. Plate film, a sealing film used for PCR reaction plates, includes a polymer sheet. The polymer sheet is provided with a number of convex posts that penetrate up and down. The openings of the convex posts penetrate the polymer sheet. The side is covered with a polymer transparent film made of a transparent material. By adopting the above technical solution, the pollution caused by the sealing film adhesive to the formwork is reduced, and it has better ultraviolet transmittance and better heat resistance; at the same time, there is no need to set up an overly complicated connection structure, and the structure is simple.

technical problem

However, the sealing film of the above-mentioned technical scheme has poor sealing performance to the PCR plate, which causes the experimental reactants in the PCR plate to be easily polluted and damaged by external factors, thereby affecting the accuracy of the experimental results, and it is easy to bond when bonding. It is inconvenient to use due to position deviation, so we have improved this and proposed a strong sealing PCR sealing film.

technical solution

Aiming at the deficiencies of the prior art, the present invention provides a PCR sealing film with strong airtightness, which solves the problems that the reactants are easily polluted and damaged due to the poor airtightness of the sealing film and the problems of easy position deviation during lamination. In order to achieve the above object, the technical scheme that the present invention takes is:

A strong sealing PCR sealing film, comprising a carrier plate, the lower surface of the carrier plate is provided with a sealing film body, the lower surface of the sealing film body is evenly distributed with several sealing covers, each of the The lower surface of the sealing cover is provided with a sealing column, and the bottom of each of the sealing columns is installed with an introduction limit ring, and the upper surface of the bearing plate is fixedly provided with a mounting shell, and the center of the upper surface of the mounting shell is An adjustment mechanism is provided at the inside of the installation housing, and an inner partition is installed inside the installation housing, and a first limit mechanism is arranged inside the installation housing and above the inner partition. A second limiting mechanism is provided below the inner partition.

Preferably, the adjustment mechanism includes a pressing rod and a moving installation block, the pressing rod is movably arranged at the center of the upper surface of the installation housing, and an installation slider is fixedly installed on the lower surface of the moving installation block.

Preferably, the installation slider is slidably arranged in the limit chute, auxiliary push rods are provided on both ends of the mobile installation block, and a limit rod is fixedly installed on the surface of the side of the mobile installation block close to the pressing rod. .

As a preference, a number of limiting holes are evenly distributed on the pressing rod, a limiting plate is provided on the upper surface of the installation housing and on the side away from the moving installation block, and the pressing rod is located on the inside of the installation housing. A first clamping block is fixedly installed on the surface of one end.

As a preference, a first installation plate is fixedly installed on the side surface of the pressing rod located inside the installation housing, a first spring is provided on the lower surface of the first installation plate, and the other end of the first spring is fixedly installed on the The upper surface of the inner partition.

Preferably, the first limiting mechanism includes a first connecting plate and a second mounting plate, the first connecting plate is fixedly installed on both ends of the pressing rod inside the installation housing, and the second mounting plate is fixed It is installed on the surface of one end of the first connecting plate away from the pressing rod.

Preferably, first connecting rods are fixedly installed on both sides of the lower surface of the second mounting plate, the other end of each of the first connecting rods passes through the inner partition and a second clamping block is fixedly installed on the end, so Both sides of the bottom of the second clamping block are provided with first guide parts.

As a preference, four mounting grooves are evenly distributed on the upper surface of the bearing plate, the inner walls at both ends of each of the mounting grooves are fixedly mounted with second springs, and the other ends of each of the second springs are fixedly mounted with limited Position splints, the top side of each of the limit splints is provided with a second guide part, and the limit splints are arranged below the second clamping block.

Preferably, the second limiting mechanism includes a second connecting rod and a slot, the second connecting rod is movably installed at the center of the inner partition, the top surface of the second connecting rod is provided with a slot, A second connecting plate is fixedly installed at one end of the second connecting rod below the inner partition, and several third springs are evenly distributed on the upper surface of the second connecting plate, and the other end of the third spring Fixedly installed on the lower surface of the inner partition.

As a preference, a limit baffle is fixedly installed on the end of the second connecting plate away from the second connecting rod, and an end limit block is arranged on the bottom side of the limit baffle, and the limit baffle is arranged on The installation limit groove is arranged in the bearing plate and is located outside the main body of the sealing film.

Beneficial effect

Compared with the prior art, the present invention has the following beneficial effects:

1. Through the cooperation between the first block and the card slot, the pressing rod can push the second connecting rod to move, and then the limit baffle in the installation limit groove can be moved downward, so that the limit baffle can be Seal the main body of the sealing film and the surroundings of the PCR plate, and through the end limit block set at the bottom of the limit baffle, the limit baffle can be better bonded to the main body of the sealing film. The sealing effect of the main body of the sealing film is better, the inside of the PCR plate is not easily polluted by external factors, and the detection result is more accurate.

2. During the downward movement of the pressing rod, it will drive the first connecting plates on both sides to move. Under the action of the first connecting rod, the second clamping block can be matched with the installation groove, and the second block can pass through the installation groove. The spring is movable and installed with two limiting splints, so that the two limiting splints can clamp and limit the position of the second clamping block, so that the bonding effect between the main body of the sealing film and the PCR plate can be better. There will not be a large position deviation during the combination process, which is more convenient to use.

Description of drawings

Fig. 1 is the overall structure schematic diagram of a kind of strong sealing PCR plate sealing film of the present invention;

Fig. 2 is the specific structure schematic diagram of the sealing film main body of a kind of strong sealing PCR sealing film of the present invention;

Fig. 3 is a structural schematic diagram of another angle of the sealing film main body of a strong sealing PCR sealing film of the present invention;

Fig. 4 is an enlarged view at A in Fig. 1 of a kind of strong sealing PCR sealing film of the present invention;

Figure 5 is an enlarged view at B in Figure 3 of a strong sealing PCR sealing film of the present invention;

Figure 6 is a front view of a strong sealing PCR sealing film of the present invention;

Fig. 7 is a schematic cross-sectional view at C-C in Fig. 6 of a strong sealing PCR sealing film of the present invention;

Fig. 8 is a schematic cross-sectional view at D-D in Fig. 6 of a strong sealing PCR sealing film of the present invention;

Fig. 9 is a schematic cross-sectional view of E-E in Fig. 6 of a strong sealing PCR sealing film of the present invention;

Fig. 10 is an enlarged view of F in Fig. 8 of a strong sealing PCR sealing film of the present invention.

In the figure: 1, bearing plate; 2, main body of sealing film; 201, sealing cover; 202, sealing column; 203, introducing limit ring; 3, installing shell; 4, adjusting mechanism; 401, pressing rod; Mobile installation block; 403, slide block installation; 404, limit chute; 405, auxiliary push rod; 406, limit rod; 407, limit hole; 408, limit plate; 409, first block; 410, The first mounting plate; 411, the first spring; 5, the inner partition; 6, the first limit mechanism; 601, the first connecting plate; 602, the second mounting plate; 603, the first connecting rod; 604, the second Block; 605, first guide part; 606, installation groove; 607, second spring; 608, limit splint; 609, second guide part; 7, second limit mechanism; 701, second connecting rod; 702 , the card slot; 703, the second connecting plate; 704, the third spring; 705, the limit baffle; 706, the end limit block; 707, the installation limit groove.

Embodiments of the present invention

The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

Example

As shown in Figure 1-10, a PCR sealing film with strong sealing performance includes a carrier plate 1, the lower surface of the carrier plate 1 is provided with a sealing film main body 2, and the lower surface of the sealing film main body 2 is evenly distributed with several A sealing cover 201, the lower surface of each sealing cover 201 is provided with a sealing column 202, the bottom of each sealing column 202 is equipped with an introduction limit ring 203, and the upper surface of the carrier plate 1 is fixedly provided with an installation housing 3, An adjustment mechanism 4 is provided at the center of the upper surface of the installation housing 3 , an inner partition 5 is installed inside the installation housing 3 , and a first limit mechanism 6 is arranged inside the installation housing 3 and above the inner partition 5 , the inside of the installation housing 3 and below the inner partition 5 are provided with a second limiting mechanism 7 .

In this embodiment, the adjustment mechanism 4 includes a pressing rod 401 and a moving installation block 402, the pressing rod 401 is movably arranged at the center of the upper surface of the installation housing 3, and the lower surface of the moving installation block 402 is fixedly installed with an installation slider 403 , the installation slider 403 is slidingly arranged in the limit chute 404, the two ends of the mobile installation block 402 are provided with auxiliary push rods 405, and the side surface of the mobile installation block 402 close to the pressing rod 401 is fixedly installed with a limit rod 406, The moving installation block 402 is slidably installed by sliding the installation slider 403 arranged in the limiting chute 404, so that the limiting rod 406 is more convenient for adjustment and use.

It should be noted that a number of limiting holes 407 are evenly distributed on the pressing rod 401, a limiting plate 408 is arranged on the upper surface of the installation housing 3 and on the side away from the moving installation block 402, and the pressing rod 401 is located in the installation housing. 3 The first block 409 is fixedly installed on the surface of one end of the interior. By setting the limit plate 408, the position of the limit rod 406 can be limited when the limit rod 406 passes through the limit hole 407, so that the limit rod 406 The position of the rod 401 is not easy to change, thereby making the fixing effect of the pressing rod 401 better.

In the specific setting, the first installation plate 410 is fixedly installed on the side surface of the pressing rod 401 located inside the installation housing 3, the lower surface of the first installation plate 410 is provided with a first spring 411, and the other end of the first spring 411 is fixed. Installed on the upper surface of the inner partition 5, under the elastic action of the first spring 411, the reset work after pressing the pressing rod 401 can be completed, which is more convenient in use.

Wherein, the first limit mechanism 6 includes a first connecting plate 601 and a second mounting plate 602, the first connecting plate 601 is fixedly installed on the two ends of the pressing rod 401 inside the installation housing 3, and the second mounting plate 602 is fixed Installed on the surface of one end of the first connecting plate 601 away from the pressing rod 401, the two sides of the lower surface of the second mounting plate 602 are fixedly installed with first connecting rods 603, and the other end of each first connecting rod 603 runs through the inner partition 5 And the end is fixedly installed with the second clamping block 604, and the bottom two sides of the second clamping block 604 are all provided with the first guiding part 605, and the upper surface of the bearing plate 1 is evenly provided with four installation grooves 606, and each installation groove The inner walls at both ends of 606 are fixedly equipped with second springs 607, and the other end of each second spring 607 is fixedly installed with limiting splints 608, and the top side of each limiting splints 608 is provided with second guides 609, limiting The position splint 608 is arranged below the second clamping block 604, and the position movement of the first connecting plate 601 will drive the first connecting rod 603 to move, so that the second clamping block 604 can be matched with the installation groove 606, and the two Under the action of the position-limiting splint 608 movably installed by the second spring 607, the two position-limiting splints 608 can be clamped and limited to the position of the second clamping block 604, so that the sealing film main body 2 and the PCR board can be attached to each other. The bonding effect is better, there will be no large position deviation during the bonding process, and the use is more convenient.

It should be noted that the second limiting mechanism 7 includes a second connecting rod 701 and a card slot 702, the second connecting rod 701 is movably installed at the center of the inner partition 5, and the top surface of the second connecting rod 701 is provided with a card slot. Groove 702, the second connecting rod 701 is located at one end of the inner partition 5 and is fixedly installed with a second connecting plate 703. The upper surface of the second connecting plate 703 is evenly distributed with several third springs 704. The third spring 704 The other end of the second connecting plate 703 is fixedly installed on the lower surface of the inner partition 5, and the end of the second connecting plate 703 away from the second connecting rod 701 is fixedly installed with a limit baffle 705, and the bottom side of the limit baffle 705 is provided with an end limiter. The position block 706 and the limit baffle 705 are set in the installation limit groove 707, and the installation limit groove 707 is set in the carrier plate 1 and is located outside the main body 2 of the sealing film, passing between the first block 409 and the draw groove 702 The cooperation between them can make the pressing rod 401 push the second connecting rod 701 to move, and then the limit baffle 705 can be moved, so that the limit baffle 705 can seal the periphery of the sealing film main body 2 and the PCR plate, and the end The limit block 706 can make the limit baffle 705 fit better with the main body 2 of the sealing film, so that the sealing performance is better.

The working principle of this strong sealing PCR sealing film:

When in use, the main body 2 of the sealing film is packaged on the PCR board, and each hole on the PCR board can be individually sealed by setting several sealing posts 202, and the size of the lead-in limiting ring 203 is slightly larger than that of the sealing posts 202 , so that the sealing column 202 is not easy to pop out when receiving an external force. The upper end of each sealing column 202 is provided with a sealing cover 201, which can make the sealing effect better. When the sealing film main body 2 is packaged, press the The rod 401 is pressed down so that the first block 409 can cooperate with the draw-in groove 702, and then the pressing rod 401 can push the second connecting rod 701 to press down. When the second connecting rod 701 moves downward, the second connecting rod 701 can be moved downward. The connecting plate 703 moves downward, and then the limit baffle 705 in the installation limit groove 707 can be moved downward, so that the limit baffle 705 can seal the surroundings of the sealing film main body 2 and the PCR plate. The end limit block 706 at the bottom of the limit baffle 705 can make the limit baffle 705 fit better with the main body 2 of the sealing film, so that the sealing effect of the main body 2 of the sealing film is better. The inside of the PCR board is not easily polluted by external factors, which can make the detection result more accurate. When the pressing rod 401 moves downward, it will also drive the first connecting plates 601 on both sides to move, so that the first connecting plate 601 installed on the second The first connecting rod 603 on the lower surface of the mounting plate 602 moves so that the second block 604 at the bottom of the first connecting rod 603 can cooperate with the mounting groove 606, and there are two limiters installed in the mounting groove 606 through the second spring 607. The position splint 608, under the elastic action of the spring, can make the two limit splints 608 clamp and limit the position of the second block 604, so that the bonding effect between the sealing film main body 2 and the PCR plate can be better , there will not be a large position deviation during the bonding process, and it is more convenient to use. When the pressing rod 401 is pressed to the designated position, the auxiliary push rod 405 is used to push the mobile installation block 402 to move, so that the limit rod 406 wears By pressing the designated limit hole 407 on the rod 401, the position of the pressing rod 401 can be limited and fixed, so that the first limit mechanism 6 and the second limit mechanism 7 can limit the position of the sealing film main body 2 It is more stable, so that the sealing effect of the sealing film main body 2 is better.

The basic principles and main features of the present invention and the advantages of the present invention have been shown and described above. Those skilled in the industry should understand that the present invention is not limited by the above-mentioned embodiments. What are described in the above-mentioned embodiments and the description only illustrate the principle of the present invention. Without departing from the spirit and scope of the present invention, the present invention will also have Variations and improvements are possible, which fall within the scope of the claimed invention. The protection scope of the present invention is defined by the appended claims and their equivalents.

Claims

A strong sealing PCR sealing film, comprising a carrier plate (1), characterized in that: the lower surface of the carrier plate (1) is provided with a sealing film main body (2), and the sealing film main body (2) Several sealing covers (201) are evenly distributed on the lower surface of the sealing cover (201), and sealing posts (202) are provided on the lower surface of each sealing cover (201), and the bottom of each sealing post (202) is installed with The limit ring (203) is introduced, the upper surface of the bearing plate (1) is fixedly provided with an installation housing (3), and the center of the upper surface of the installation housing (3) is provided with an adjustment mechanism (4), so An inner partition (5) is installed inside the installation housing (3), and a first limit mechanism (6) is arranged inside the installation housing (3) and above the inner partition (5). A second limiting mechanism (7) is provided inside the installation housing (3) and below the inner partition (5).
A strong sealing PCR sealing film according to claim 1, characterized in that: the adjustment mechanism (4) includes a pressing rod (401) and a moving installation block (402), and the pressing rod (401) The movable installation is arranged at the center of the upper surface of the installation housing (3), and the lower surface of the movable installation block (402) is fixedly installed with an installation slider (403).
A strong sealing PCR sealing film according to claim 2, characterized in that: the installation slider (403) is slidably set in the limit chute (404), and the moving installation block (402) Auxiliary push rods (405) are provided on both ends of the surface, and a limiting rod (406) is fixedly installed on the surface of the side of the moving installation block (402) close to the pressing rod (401).
A strong sealing PCR plate sealing film according to claim 3, characterized in that: several limit holes (407) are evenly distributed on the pressing rod (401), and the installation shell (3) A limiting plate (408) is provided on the upper surface of the upper surface and away from the moving installation block (402), and the first clamping block (409) is fixedly installed on the surface of one end of the pressing rod (401) inside the installation housing (3) ).
A strong sealing PCR sealing film according to claim 4, characterized in that: the first mounting plate (410) is fixedly installed on the side surface of the pressing rod (401) located inside the mounting shell (3) , the lower surface of the first installation plate (410) is provided with a first spring (411), and the other end of the first spring (411) is fixedly installed on the upper surface of the inner partition (5).
A strong sealing PCR sealing film according to claim 2, characterized in that: the first limiting mechanism (6) includes a first connecting plate (601) and a second mounting plate (602), so The first connecting plate (601) is fixedly installed on the surface of both ends of the pressing rod (401) inside the installation housing (3), and the second installing plate (602) is fixedly installed on the first connecting plate (601) away from the pressing One end surface of the rod (401).
A strong sealing PCR sealing film according to claim 6, characterized in that: first connecting rods (603) are fixedly installed on both sides of the lower surface of the second mounting plate (602), and each of the The other end of the first connecting rod (603) passes through the inner partition (5) and the end is fixedly installed with a second clamping block (604), and both sides of the bottom of the second clamping block (604) are provided with first guide part (605).
A strong sealing PCR plate sealing film according to claim 7, characterized in that four mounting grooves (606) are evenly distributed on the upper surface of the carrier plate (1), and each of the mounting grooves ( 606) are fixedly installed with a second spring (607) on the inner wall at both ends, and the other end of each second spring (607) is fixedly installed with a limiting splint (608), and each of the limiting splints (608) A second guide part (609) is provided on one side of the top, and the limiting splint (608) is arranged under the second clamping block (604).
A strong sealing PCR plate sealing film according to claim 1, characterized in that: the second limiting mechanism (7) includes a second connecting rod (701) and a slot (702), the first Two connecting rods (701) are movably installed at the center of the inner partition (5), the top surface of the second connecting rod (701) is provided with a slot (702), and the second connecting rod (701) is located in the inner One end below the partition (5) is fixedly installed with a second connecting plate (703), and several third springs (704) are evenly distributed on the upper surface of the second connecting plate (703). The other end of the spring (704) is fixedly installed on the lower surface of the inner partition (5).
The strong sealing PCR sealing film according to claim 9, characterized in that: the end of the second connecting plate (703) away from the second connecting rod (701) is fixedly installed with a limit baffle (705) , the bottom side of the limit baffle (705) is provided with an end limit block (706), the limit baffle (705) is set in the installation limit groove (707), the installation limit The groove ( 707 ) is arranged in the bearing plate ( 1 ) and is located outside the sealing film main body ( 2 ).