WO2023093382A1 - Système de détection microfluidique pour réfrigérateur et réfrigérateur - Google Patents

Système de détection microfluidique pour réfrigérateur et réfrigérateur Download PDF

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Publication number
WO2023093382A1
WO2023093382A1 PCT/CN2022/126153 CN2022126153W WO2023093382A1 WO 2023093382 A1 WO2023093382 A1 WO 2023093382A1 CN 2022126153 W CN2022126153 W CN 2022126153W WO 2023093382 A1 WO2023093382 A1 WO 2023093382A1
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WO
WIPO (PCT)
Prior art keywords
microfluidic
biochip
microfluidic biochip
detection system
elastic airbag
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PCT/CN2022/126153
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English (en)
Chinese (zh)
Inventor
费斌
赵斌堂
李孟成
Original Assignee
青岛海尔电冰箱有限公司
海尔智家股份有限公司
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Application filed by 青岛海尔电冰箱有限公司, 海尔智家股份有限公司 filed Critical 青岛海尔电冰箱有限公司
Publication of WO2023093382A1 publication Critical patent/WO2023093382A1/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
    • F25D23/00—General constructional features
    • F25D23/12—Arrangements of compartments additional to cooling compartments; Combinations of refrigerators with other equipment, e.g. stove
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices

Definitions

  • the invention relates to refrigerating and freezing technology, in particular to a microfluidic detection system for a refrigerator and the refrigerator.
  • the detection method using microfluidic biochip is relatively fast and small in size, which is suitable for home use.
  • pneumatic push type In order to promote the movement of the fluid in the chip, there are usually two kinds of pneumatic push type and centrifugal force push type.
  • the centrifugal force pushes the droplet to flow by means of the rotating centrifugal force, and the one-way flow action can only be adjusted by adjusting the rotational speed.
  • the pneumatic push type uses positive and negative air pressure to bidirectionally push the movement of the fluid in the chip, with high precision and strong controllability.
  • An object of the first aspect of the present invention is to overcome at least one defect of the prior art and provide a microfluidic detection system suitable for refrigerators with good sealing performance and precise sample injection control.
  • a further object of the first aspect of the present invention is to simplify assembly operations while ensuring good airtightness.
  • the object of the second aspect of the present invention is to provide a refrigerator with the above-mentioned microfluidic detection system.
  • the present invention provides a microfluidic detection system for a refrigerator, which includes:
  • the microfluidic biochip has a sample inlet, an air suction port formed at its end, and a detection pool formed inside it, and a micro The flow channels are sequentially connected;
  • An elastic airbag configured to be in sealing contact with the end of the microfluidic biochip formed with the suction port, and the inner space of the elastic airbag communicates with the suction port;
  • a sample liquid driving device configured to squeeze and release the elastic airbag in a controlled manner, so as to force the sample liquid in contact with the injection port to enter the micro-channel and pass through the elastic airbag during the process of restoring deformation of the elastic airbag; said microchannel flows to said detection cell;
  • the detection mechanism is used to detect the detection cell, so as to obtain the preset detection parameters of the sample liquid.
  • the elastic airbag is hermetically sleeved on the outside of the end of the microfluidic biochip formed with the suction port.
  • the elastic balloon has a spherical balloon portion and a connection end for connecting with the microfluidic biochip;
  • the sample liquid driving device is configured to apply opposing pressing forces to the spherical air bag part from two opposite directions, so as to promote the elastic deformation of the spherical air bag part.
  • the sample inlet is formed at the bottom port of the microfluidic biochip
  • the suction port is formed at the top port of the microfluidic biochip
  • the elastic airbag is connected to the microfluidic biochip. control the top of the biochip, and hermetically socketed on the outside of the top port;
  • the sample fluid driving device is configured to controllably apply a squeezing force parallel to the horizontal plane to the spherical balloon portion.
  • the sample fluid driving device includes:
  • a driving motor for controllingly outputting a driving force
  • the screw is connected with the output shaft of the drive motor, and is used to rotate under the drive of the drive motor;
  • the screw has a forward thread section and a reverse thread section, and the forward thread section and the forward and reverse threads in opposite directions are respectively provided on the reverse thread section;
  • the two sliders are respectively located on opposite sides of the elastic airbag.
  • the microfluidic detection system also includes:
  • the installation mechanism has an installation groove for inserting the microfluidic biochip and a cavity for accommodating the elastic airbag, the notch of the installation groove and the opening of the cavity face the same direction;
  • the integral body formed by the microfluidic biochip and the elastic airbag is installed into the installation mechanism through the notch of the installation groove and the opening of the cavity so that the microfluidic biochip is inserted into the installation In the groove, the elastic airbag is accommodated in the cavity, and the sample inlet of the microfluidic biochip is outside the installation groove.
  • the sample inlet is formed at the bottom port of the microfluidic biochip
  • the suction port is formed at the top port of the microfluidic biochip
  • the elastic airbag is connected to the microfluidic biochip. control the top of the biochip, and hermetically socketed on the outside of the top port;
  • the installation groove extends vertically, the cavity is connected above the installation groove, and the integral configuration formed by the microfluidic biochip and the elastic airbag is installed to the installation along a direction parallel to the horizontal plane. mechanism.
  • the size of the installation groove is smaller than the size of the cavity, so as to form a step at the boundary between the installation groove and the cavity;
  • the size of the top port of the microfluidic biochip in the thickness direction of the microfluidic biochip of the microfluidic biochip is larger than the thickness of the microfluidic biochip, and the bottom of the top port abuts against the step portion.
  • the installation mechanism also has at least one clamping member disposed in the installation groove, and the clamping member is configured to clamp the microfluidic biochip after the microfluidic biochip is inserted into the installation groove. Fluidic biochip.
  • the clamping member includes two symmetrical and spaced clamping jaws, and the two clamping jaws are configured to respectively move toward the microfluidic biochip after the microfluidic biochip is installed Two opposite side surfaces of the biochip apply opposing elastic forces.
  • the microfluidic biochip is molded by injection molding; and/or
  • the elastic airbag is molded by silica gel or PE blow molding.
  • the present invention also provides a refrigerator, which includes the microfluidic detection system described in any of the above solutions.
  • an elastic airbag is specially sealed and connected to the end of the microfluidic biochip formed with the suction port, so that there is no need for a connecting pipeline between the sample liquid driving device and the microfluidic biochip, and only a simple mechanical squeeze is done.
  • pressure that is, there is no air-tightness problem between the two, so that the air-tightness problem between the sample liquid driving device and the microfluidic biochip is transferred to the gap between the elastic airbag and the end of the microfluidic biochip.
  • Air tightness problem It is understandable that the sealing between the microfluidic biochip and the sample liquid driving device is completed during or after the installation of the microfluidic biochip, and the operating space and operation methods are relatively limited, and the sealing effect cannot be guaranteed.
  • the sealing connection between the elastic airbag and the microfluidic biochip of the present invention is completed before the installation of the microfluidic biochip, and there is no restriction on the operating space and the sealing method. Therefore, the connection between the elastic airbag and the microfluidic biochip An effective, good seal can be achieved.
  • the present invention controls the deformation of the elastic airbag through the sample liquid driving device to control the liquid absorption and exhaust volume, which not only eliminates the airtight problem between the sample liquid driving device and the microfluidic biochip, but also maintains the advanced The accuracy of sample control.
  • the inventors realized that since the elastic airbag has the property of elastic expansion and contraction, this property can be used to form a sealed connection between the elastic airbag and the end of the microfluidic biochip. For this reason, the present invention seals the elastic airbag on the outside of the end of the microfluidic biochip formed with the suction port, and the elastic airbag tightly binds the microfluidic biochip. The assembly connection between them, but also to ensure the seal between the two.
  • the invention integrates the microfluidic detection system on the refrigerator, fully utilizes the storage function of the refrigerator, makes the detection process more convenient, and facilitates the linkage control of the microfluidic detection system and the refrigerator. Smart home needs.
  • Fig. 1 is a schematic structural diagram of a microfluidic detection system for a refrigerator according to an embodiment of the present invention
  • FIG. 2 is a schematic structural diagram of the internal structure of a microfluidic detection system according to an embodiment of the present invention
  • FIG. 3 is a schematic diagram of a partial structure of a microfluidic detection system according to an embodiment of the present invention.
  • Fig. 4 is a schematic exploded view of a partial structure of a microfluidic detection system according to an embodiment of the present invention
  • Fig. 5 is a schematic cross-sectional view of a microfluidic biochip and an elastic airbag according to an embodiment of the present invention
  • Fig. 6 is a schematic structural exploded view of a microfluidic biochip and an elastic airbag according to an embodiment of the present invention
  • Fig. 7 is a schematic structural diagram of a refrigerator according to an embodiment of the present invention.
  • the present invention firstly provides a microfluidic detection system for refrigerators.
  • the microfluidic detection system of the present invention is used for qualitative or quantitative detection of the preset detection parameters of the sample liquid.
  • the preset detection parameters can be, for example, used for Pesticide parameters indicating whether the amount of pesticide residues exceeds the standard and/or the specific value of the amount of pesticide residues, nutritional parameters used to indicate whether the nutrient elements are up to the standard and/or the specific content of nutrient elements, and used to indicate whether specific harmful substances (such as specific viruses) Specific substance parameters in excess and/or specified levels, etc.
  • FIG. 1 is a schematic structural diagram of a microfluidic detection system for a refrigerator according to an embodiment of the present invention
  • Fig. 2 is a schematic structural diagram of the internal structure of a microfluidic detection system according to an embodiment of the present invention
  • Fig. 3 is a schematic diagram of a partial structure of a microfluidic detection system according to an embodiment of the present invention
  • FIG. 4 is a schematic exploded view of a partial structure of a microfluidic detection system according to an embodiment of the present invention.
  • the sample cup 2 is also shown in FIGS. 1 to 2 .
  • the microfluidic detection system 1 of the present invention includes a microfluidic biochip 10 , an elastic airbag 60 , a sample fluid driving device 40 and a detection mechanism 20 .
  • Fig. 5 is a schematic cross-sectional view of a microfluidic biochip and an elastic airbag according to an embodiment of the present invention
  • Fig. 6 is a schematic structural exploded view of a microfluidic biochip and an elastic airbag according to an embodiment of the present invention.
  • the microfluidic biochip 10 has a sample inlet 111, an air suction port 112 formed at its end, and a detection pool 113 formed inside it.
  • the flow passages 114 are sequentially connected to form a main passage.
  • the elastic airbag 60 is configured to be in sealing contact with the end of the microfluidic biochip 10 formed with the suction port 112 , and the inner space of the elastic airbag 60 communicates with the suction port 112 .
  • the sample liquid driving device 40 is configured to squeeze and release the elastic airbag 60 in a controlled manner, so as to promote the sample liquid in contact with the sample inlet 111 to enter the microchannel 114 and flow to the Detection pool 113.
  • the detection mechanism 20 is used to detect the detection cell 113 to obtain preset detection parameters of the sample fluid. Specifically, a detection reagent can be provided in the detection pool 113, so that the detection pool 113 can be detected by the detection mechanism 20 after the sample liquid in the detection pool 113 reacts with the detection reagent therein.
  • the microfluidic detection system 1 of the present invention includes a microfluidic biochip 10 and an elastic airbag 60 that is in sealing contact with the end of the microfluidic biochip 10 formed with a suction port 112.
  • the suction port 112 of the fluidic biochip 10 is connected to form a closed space between the microfluidic biochip 10 and the elastic airbag 60 , and only the inlet 111 is used for sample injection.
  • the sample liquid driving device 40 discharges the air in the microfluidic biochip 10 by squeezing the elastic air bag 60. When the sample liquid driving device 40 releases the elastic air bag 60, the elastic air bag 60 resumes its deformation, thereby prompting the sample in contact with the injection port 111 to The liquid enters the detection pool in the microfluidic biochip 10.
  • an elastic airbag 60 is specially sealed and connected to the end of the microfluidic biochip 10 where the suction port 112 is formed, and no communication pipeline is needed between the sample liquid driving device 40 and the microfluidic biochip 10, only Do simple mechanical extrusion, that is, there is no air-tightness problem between the two, so that the air-tightness problem between the sample liquid driving device 40 and the microfluidic biochip 10 is transferred to the elastic airbag 60 and the microfluidic biochip. The problem of airtightness between the ends of the biochip 10 .
  • the sealing between the microfluidic biochip 10 and the sample solution driving device 40 is completed when the microfluidic biochip 10 is installed or after installation, and the operating space and operating methods are relatively limited, and the sealing effect is relatively large. Not guaranteed.
  • the sealing connection between the elastic airbag 60 and the microfluidic biochip 10 of the present invention is completed before the installation of the microfluidic biochip 10, and there is no restriction on the operating space and the sealing method. Therefore, the elastic airbag 60 and the microfluidic biochip Effective and good sealing can be achieved between the biochips 10 .
  • the present invention controls the amount of deformation of the elastic airbag 60 through the sample liquid driving device 40 to control the liquid absorption and exhaust volume, which not only eliminates the airtightness problem between the sample liquid driving device 40 and the microfluidic biochip 10, but also The precision of injection control is also maintained.
  • the microfluidic biochip 10 when the preset detection parameters used by the microfluidic detection system are different, the specific selection of the microfluidic biochip 10 and the detection mechanism 20 used therein may also be different.
  • the microfluidic detection system when used for pesticide residue detection, the microfluidic biochip 10 it has can be a microfluidic pesticide detection chip that can provide detection conditions for the pesticide residue liquid, and the detection mechanism 20 it has can be It is a pesticide residue detection mechanism that can detect the pesticide residue parameters of the pesticide residue liquid.
  • the microfluidic biochip 10 when the detection mechanism 20 is a pesticide residue detection mechanism for detecting the pesticide residue parameters of the pesticide residue liquid, the enzyme inhibition rate method can be used to quickly determine whether the pesticide residue in the sample liquid exceeds the standard. detection.
  • the microfluidic biochip 10 also includes a reaction pool 115 formed inside it, and the reaction pool 115 is located on the main channel formed by connecting the sample inlet 111, the detection pool 113, and the suction port 112 in sequence, and communicates with the inlet port 111. Between the sample port 111 and the detection cell 113 , so that the sample liquid first reacts with the reaction reagent in the reaction cell 115 and then flows into the detection cell 113 .
  • Both the reaction pool 115 and the sample inlet 111 , and the reaction pool 115 and the detection pool 113 are communicated through microchannels 114 .
  • the reaction reagent and detection reagent used for pesticide residue detection can be enzyme reagent and chromogenic reagent respectively.
  • the reaction pool 115 is used for reacting the sample liquid with the enzyme reagent therein, and the sample liquid reacted with the enzyme reagent flows into the detection pool 113 to react with the color developer in the detection pool 113 .
  • the detection mechanism 20 can be selected as a photoelectric detection mechanism, which can include structures such as a light source, a photosensitive element, a heating plate, and a temperature controller.
  • the elastic airbag 60 has the property of elastic expansion and contraction, this property can be used to form a sealed connection between the elastic airbag 60 and the end of the microfluidic biochip 10 .
  • the elastic airbag 60 is hermetically sleeved outside the end of the microfluidic biochip 10 where the suction port 112 is formed, and the elastic airbag 60 tightly binds the microfluidic biochip 10, Not only simply realize the assembly connection between the two, but also ensure the sealing between the two.
  • microfluidic biochip 10 can be molded by injection molding, so as to add reagents in its detection pool 113 and reaction pool 115 .
  • the elastic airbag 60 can be molded by silica gel or PE blow molding.
  • the elastic airbag made of this kind of material has better elastic deformation ability and stronger deformation recovery ability, and is very suitable for promoting fluid flow in the microchannel.
  • the elastic balloon 60 has a spherical balloon portion 61 and a connection end 62 for connecting with the microfluidic biochip 10 .
  • the sample solution driving device 40 is configured to exert opposing pressing force toward the spherical air bag portion 61 of the elastic air bag 60 from two opposite directions, so as to promote the elastic deformation of the spherical air bag portion 61 .
  • the force received by the elastic airbag 60 from the sample solution driving device 40 is more balanced, so its deformation is more uniform and easier to control, so that it is convenient to control the amount of deformation of the elastic airbag 60 to realize the control of the liquid intake. Precise control.
  • the spherical airbag portion 61 if the spherical airbag portion 61 is subjected to an unbalanced force, the spherical airbag portion 61 will be deformed and its center will deviate from the original position to produce an inclination, which will cause the elastic airbag 60 to be in contact with the microfluidic biochip.
  • the displacement of the joint between 10 affects the sealing effect between the two, and even causes the elastic airbag 60 to break away from the microfluidic biochip 10 .
  • the sample solution driving device 40 of the present invention applies opposing extrusion forces to the spherical airbag part 61 from two opposite directions. During the extrusion process, although the spherical airbag part 61 is deformed, the position of its center remains unchanged. Therefore, the sealing effect between the elastic air bag 60 and the sample liquid driving device 40 will not be affected.
  • the sample inlet 111 is formed at the bottom port of the microfluidic biochip 10
  • the suction port 112 is formed at the top port 12 of the microfluidic biochip 10
  • the elastic airbag 60 is connected to the microfluidic biochip 10. above, and sealingly sleeved on the outside of the top port 12.
  • the sample solution driving device 40 is configured to apply a pressing force parallel to the horizontal plane to the spherical balloon portion 61 in a controlled manner.
  • the elastic airbag 60 has a connection end 62 connected with the microfluidic biochip 10, the connection end 62 is equivalent to one pole of the spherical airbag part 61, and the upper end of the spherical airbag part 61 opposite to the connection end is equivalent to The other pole of the spherical airbag part 61, therefore, the spherical airbag part 61 is easier to deform in the equatorial direction parallel to the horizontal direction, and the spherical airbag part 61 is more likely to recover after being deformed in this direction. Therefore, the sample liquid driving device 40 applies a pressing force parallel to the horizontal plane to the spherical air bag portion 61 to facilitate the deformation and restoration of the spherical air bag portion 61 .
  • the sample solution driving device 40 specifically includes a driving motor 41 , a screw 42 and two sliders 43 .
  • the driving motor 41 is used to output driving force in a controlled manner.
  • the screw rod 42 is connected with the output shaft of the driving motor 41 and is used to rotate under the drive of the driving motor 41 .
  • the screw 42 has a forward thread section and a reverse thread section, and the forward thread section and the reverse thread section are respectively provided with a forward thread and a reverse thread in opposite directions.
  • the two sliders 43 are sheathed on the screw rod 42 and are threadedly connected with the forward thread section and the reverse thread section of the screw rod 42 respectively, so as to move toward or away from each other when the screw rod 42 rotates.
  • the two sliders 43 are respectively located on opposite sides of the elastic airbag 60 .
  • the drive motor 41 rotates forward under control
  • the screw 42 rotates forward
  • the two sliders 43 can translate on the screw 42 toward each other, thereby pressing the elastic airbag 60 toward each other, thereby causing The elastic airbag 60 is deformed.
  • the driving motor 41 reverses in a controlled manner
  • the screw 42 rotates in the opposite direction
  • the two sliders 43 can translate on the screw 42 in a direction away from each other, thereby releasing the elastic airbag 60 .
  • the elastic airbag 60 recovers its deformation under the action of its elastic deformation restoring force.
  • the sample solution driving device 40 can stably and finely control the sliding of the two sliders 43 through the driving motor 41 , thereby accurately and reliably controlling the deformation of the elastic airbag 60 .
  • the microfluidic detection system 1 further includes a mounting mechanism 30 .
  • the applicant realized that since there is no airtight problem between the microfluidic biochip 10 and the sample solution driving device 40, that is, there is no need to consider the connection between the microfluidic biochip 10 and the sample solution driving device 40 when the microfluidic biochip 10 is installed.
  • the sealed docking structure only needs to ensure that the microfluidic biochip 10 remains stable and reliable after installation. Therefore, the installation mechanism 30 of the present invention does not need to design a very complicated structure, but only needs to be able to hold the microfluidic biochip 10 .
  • the mounting mechanism 30 of the present invention has a mounting groove 31 for inserting the microfluidic biochip 10 and a cavity 32 for accommodating the elastic airbag 60, and the notch of the mounting groove 31 and the opening of the cavity 32 face same direction.
  • the whole formed by the microfluidic biochip 10 and the elastic airbag 60 is installed in the installation mechanism 30 through the notch of the installation groove 31 and the opening of the cavity 32 so that the microfluidic biochip 10 is inserted into the installation groove 31, so that the elastic airbag 60 Accommodating in the cavity 32 not only realizes the effective installation of the microfluidic biochip 10 , but also simplifies the structure of the microfluidic detection system 1 to a large extent.
  • sample inlet 111 of the microfluidic biochip 10 is located outside the installation groove 31 , so that the sample inlet 111 can absorb the sample liquid when the microfluidic biochip 10 is in the installed state.
  • sample solution driving device 40 can be supported on the mounting mechanism 30 , and its two sliders 43 are also located in the cavity 32 so as to squeeze the elastic airbag 60 .
  • the sample inlet 111 is formed at the bottom port of the microfluidic biochip 10
  • the suction port 112 is formed at the top port 12 of the microfluidic biochip 10
  • the elastic airbag 60 is connected to the microfluidic biochip 10. above, and sealingly sleeved on the outside of the top port 12. Since the elastic airbag 60 is elastically shrinkable and deformable, it is difficult for the microfluidic biochip 10 to be installed from bottom to top.
  • the present invention further arranges the installation groove 31 to extend vertically, the cavity 32 is connected above the installation groove 31, and the integral configuration formed by the microfluidic biochip 10 and the elastic airbag 60 is installed along a direction parallel to the horizontal plane. to the mounting mechanism 30. That is to say, the elastic airbag 60 is installed in parallel with the microfluidic biochip 10, and the elastic airbag 60 will not have any hindrance or influence on the assembly of the microfluidic biochip 10, and can also pass through the structure of the installation groove 31 and the cavity 32.
  • the design only makes the microfluidic biochip 10 remain stationary in the installation groove 31 , allowing the elastic airbag 60 to produce elastic deformation in the cavity 32 without hindrance.
  • the size of the installation groove 31 is smaller than that of the cavity 32 to form a stepped portion 33 at the boundary between the installation groove 31 and the cavity 32 .
  • the size of the top port 12 of the microfluidic biochip 10 in the thickness direction of the microfluidic biochip 10 is greater than the thickness of the microfluidic biochip 10, so that the bottom of the top port 12 abuts against the step portion 33 to avoid microfluidic biochip 10.
  • the fluidic biochip 10 falls down.
  • the microfluidic biochip 10 and the elastic airbag 60 can be jointly supported on the step portion 33 , realizing the vertical positioning of the microfluidic biochip 10 .
  • the present invention utilizes the simple design of the structural dimensions of the installation groove 31 and the cavity 32 to position the microfluidic biochip 10 in the vertical direction, and the structure is very simple.
  • the installation mechanism 30 also has at least one clamping member 34 disposed in the installation groove 31, and the clamping member 34 is configured to clamp the microfluidic biochip after the microfluidic biochip 10 is inserted into the installation groove 31. 10, so as to prevent the microfluidic biochip 10 from tilting, shaking or detaching from the installation groove 31 during the process of the sample liquid driving device 40 squeezing or releasing the elastic airbag 60, so as to adjust the microfluidic biochip 10 in the horizontal direction. Carry out limit.
  • an accommodating space for accommodating the clamping member 34 may be formed in the installation groove 31, and the clamping member 34 is limited in the accommodating space and can be elastically deformed within a certain range to maintain its alignment with the chip main body. 11 Better clamping force.
  • the clamping member 34 includes two symmetrical and spaced clamping jaws 341, and the two clamping jaws 341 are configured to face the two sides of the microfluidic biochip 10 after the microfluidic biochip 10 is installed in the installation groove 31. Opposite side surfaces apply opposing elastic forces to more stably hold the microfluidic biochip 10 .
  • the microfluidic detection system 1 further includes a weighing platform 81 and a bracket 82 .
  • the weighing platform 81 is fixedly arranged on a supporting frame 83 and is used for measuring the weight of the sample contained in the sample cup 2 placed on it. It can be understood that the weighing platform 81 can measure the sum of the weight of the sample cup 2 and the sample contained therein, and subtract the weight of the sample cup 2 itself to obtain the weight of the sample.
  • the weighing platform 81 can also be set to directly detect the weight of the sample contained in the sample cup 2, such as tare measurement.
  • the carriage 82 is configured to move in a controlled or operable manner to drive the sample cup 2 to the highest position allowing the sample liquid in the sample cup 2 to contact the sample inlet 111 of the microfluidic biochip 10 .
  • the microfluidic detection system 1 further includes a buffer bottle 51 and a buffer driving device 52 .
  • the buffer bottle 51 is used for containing the buffer.
  • the buffer driving device 52 communicates with the buffer bottle 51 to controlly drive the buffer in the buffer bottle 51 into the sample cup 2 placed on the weighing platform 81, so that the buffer is mixed with the sample in the sample cup 2 A sample fluid is then produced.
  • the buffer driving device 52 may be a peristaltic pump, a diaphragm pump or other suitable driving devices.
  • the microfluidic detection system 1 further includes a housing 90 .
  • the housing 90 is formed with an operating platform open towards its front side, and the weighing platform 81 is at least partially located in the operating platform, so that it is convenient for the user to perform operations such as placing the sample cup 2 and taking out the sample cup 2 in the operating platform.
  • the microfluidic detection system 1 of the present invention is particularly provided with a weighing table 81 fixed on a support frame 83 and a bracket 82 capable of driving the sample cup 2 to move.
  • a weighing table 81 fixed on a support frame 83 and a bracket 82 capable of driving the sample cup 2 to move.
  • the user only needs to place the sample cup 2 on the weighing platform 81, the weighing platform 81 measures the weight of the sample, and the buffer drive device 52 adds an appropriate amount of buffer solution to the sample cup 2, and the bracket 82 can automatically
  • the ground drives the sample cup 2 to move to add the sample to the microfluidic biochip 10, the sample adding operation is very convenient, saves time and effort, and the user experience is better.
  • the weighing platform 81 of the present invention is fixed, and it will not move with the movement of the bracket 82, therefore, the movement of the bracket 82 will not produce any impact on the weighing accuracy of the weighing platform 81.
  • the impact ensures high-precision measurement of the weight of the sample, thereby improving the accuracy of the detection results of the microfluidic biochip 10 .
  • the bracket 82 when the sample cup 2 is placed on the weighing platform 81 for weighing, the bracket 82 should be completely separated from the sample cup 2 and not in contact, so as to avoid affecting the weighing of the sample. After the weight of the sample is measured, the bracket 82 needs to hold the sample cup 2 to drive it to move together. That is to say, the bracket 82 needs to have two states of releasing the sample cup and holding the sample cup, and can automatically switch between these two states according to the detection process. In order to achieve this purpose, prior to this application, those skilled in the art generally adopted a design idea of providing a clamping mechanism for the bracket, and by controlling the action of the clamping mechanism, the bracket can release the sample cup and hold the sample cup. automatically switch between the states.
  • the clamping mechanism increases the structural complexity of the bracket, and it is necessary to reserve space for the action switching of the clamping mechanism to avoid interference or collision with other structures, which will lead to an increase in the volume of the microfluidic detection system. Not suitable for refrigerators with limited space.
  • the retention of the sample cup, especially the release of the sample cup needs to be highly consistent with the testing process, that is, when the weighing platform needs to measure the weight of the sample, it must be ensured that the clamping mechanism is in the state of releasing the sample cup; The clamping mechanism can only clamp the sample cup after the weight of the sample is measured by the platform.
  • the bracket 82 is disposed above the weighing platform 81 and includes an annular frame 821 sheathed on the outside of the sample cup 2 .
  • the bracket 82 is configured to move up and down in a controlled or operable manner, and when moving upwards, the annular frame 821 is used to hold the sample cup 2 so that the sample cup 2 leaves the weighing platform 81, and moves downward to the lowest position.
  • the sample cup 2 is supported on the weighing platform 81 and the contact between the sample cup 2 and the weighing platform 81 is used to promote the separation of the sample cup 2 from the ring frame 821 .
  • the annular frame 821 can naturally hold up the sample cup 2 so that it leaves the weighing platform 81; when the bracket 82 moves downward to a certain position, the sample cup 2 supports On the weighing platform 81, when the bracket 82 continues to move downward to the lowest position, the abutment between the sample cup 2 and the weighing platform 81 is used to promote the separation of the sample cup 2 from the ring frame 821, thus, the bracket 82 The rack 82 will not have any influence on the weight detection of the sample.
  • bracket 82 of the present invention has completed the natural switching between the holding and releasing of the sample cup 2 in its lifting process, and there is no need to design any control program for holding up or releasing, not only the structure of the bracket 82 is very Simple, and the control logic of the bracket 82 is also very simple.
  • FIG. 7 is a schematic structural diagram of a refrigerator according to an embodiment of the present invention.
  • the refrigerator 100 of the present invention includes the microfluidic detection system 1 involved in any of the above embodiments, so that the microfluidic detection system 1 is integrated on the refrigerator 100 .
  • the refrigerator 100 is frequently used in daily life, and the refrigerator 100 is mainly used to store food materials.
  • the microfluidic detection system 1 is integrated on the refrigerator 100, it is convenient for users to use the microfluidic detection system 1 to carry out the detection of food samples. Detect operation.
  • the present invention integrates the microfluidic detection system 1 on the refrigerator 100, fully utilizes the storage function of the refrigerator 100, makes the detection process more convenient, and facilitates the linkage control between the microfluidic detection system 1 and the refrigerator 100, and the degree of intelligence Higher, meeting the needs of smart families.
  • the refrigerator 100 further includes a box body 200 and a door body 300 , a storage space is defined in the box body 200 , and the door body 300 is connected to the box body 200 and used to open and/or close the storage space.
  • the microfluidic detection system 1 is preferably installed on the door body 300 , which is not only convenient to operate, but also does not occupy the original storage space in the box body 200 and does not affect the storage capacity of the refrigerator 100 itself.
  • the refrigerator 100 of the present application is a refrigerator in a broad sense, which includes not only the so-called refrigerator in the narrow sense, but also storage devices with refrigeration, freezing or other storage functions, such as refrigerators, freezers and so on.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Thermal Sciences (AREA)
  • Mechanical Engineering (AREA)
  • Biochemistry (AREA)
  • Combustion & Propulsion (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne un système de détection microfluidique (1) pour réfrigérateur, et un réfrigérateur (100). Le système de détection microfluidique (1) comprend : une biopuce microfluidique (10), qui est pourvue d'une entrée d'échantillon (111), d'un orifice d'aspiration d'air (112) formé au niveau d'une partie d'extrémité de la biopuce, et d'une cellule de détection (113) formée dans la biopuce, l'entrée d'échantillon (111), la cellule de détection (113), et l'orifice d'aspiration d'air (112) étant en communication séquentielle les uns avec les autres au moyen d'un microcanal (114) ; une poche d'air élastique (60), qui est conçue pour être en liaison étanche avec la partie d'extrémité de la biopuce microfluidique (10) pourvue de l'orifice d'aspiration d'air (112), un espace interne de la poche d'air élastique (60) étant en communication avec l'orifice d'aspiration d'air (112) ; un dispositif d'entraînement de liquide d'échantillon (40), qui est conçu pour presser et libérer la poche d'air élastique (60) sous commande, de façon à pousser un échantillon liquide en contact avec l'entrée d'échantillon (111) à entrer dans le microcanal (114) et s'écoule vers la cellule de détection (113) à travers ledit microcanal (114) pendant le processus de restauration de la déformation de la poche d'air élastique (60) ; et un mécanisme de détection (20), qui est utilisé pour détecter la cellule de détection (113).
PCT/CN2022/126153 2021-11-25 2022-10-19 Système de détection microfluidique pour réfrigérateur et réfrigérateur WO2023093382A1 (fr)

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CN202111414287.9 2021-11-25

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CN102671729A (zh) * 2012-05-07 2012-09-19 博奥生物有限公司 一种用于多指标生化检测的微流控芯片
CN204514801U (zh) * 2015-04-01 2015-07-29 杭州霆科生物科技有限公司 一种用于农药残留现场检测的可抛型微流控芯片
CA3017978A1 (fr) * 2015-04-24 2016-10-27 Mesa Biotech, Inc. Cassette d'essai fluidique
CN205731290U (zh) * 2015-12-23 2016-11-30 杭州霆科生物科技有限公司 一种预存储反应试剂的食品安全检测用微流控芯片
CN214039171U (zh) * 2020-09-27 2021-08-24 青岛海尔电冰箱有限公司 用于冰箱的微流控检测系统及冰箱
CN214041434U (zh) * 2020-09-27 2021-08-24 青岛海尔电冰箱有限公司 用于冰箱的微流控检测系统及冰箱

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111514948B (zh) * 2020-04-27 2022-02-22 京东方科技集团股份有限公司 微流控芯片和检测系统
CN113306814A (zh) * 2021-05-18 2021-08-27 广州缘润科电商有限公司 一种洗衣液自动灌装装置

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102671729A (zh) * 2012-05-07 2012-09-19 博奥生物有限公司 一种用于多指标生化检测的微流控芯片
CN204514801U (zh) * 2015-04-01 2015-07-29 杭州霆科生物科技有限公司 一种用于农药残留现场检测的可抛型微流控芯片
CA3017978A1 (fr) * 2015-04-24 2016-10-27 Mesa Biotech, Inc. Cassette d'essai fluidique
CN205731290U (zh) * 2015-12-23 2016-11-30 杭州霆科生物科技有限公司 一种预存储反应试剂的食品安全检测用微流控芯片
CN214039171U (zh) * 2020-09-27 2021-08-24 青岛海尔电冰箱有限公司 用于冰箱的微流控检测系统及冰箱
CN214041434U (zh) * 2020-09-27 2021-08-24 青岛海尔电冰箱有限公司 用于冰箱的微流控检测系统及冰箱

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