WO2023044611A1 - Dispositif de collecte d'excréments de souris de laboratoire - Google Patents

Dispositif de collecte d'excréments de souris de laboratoire Download PDF

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Publication number
WO2023044611A1
WO2023044611A1 PCT/CN2021/119661 CN2021119661W WO2023044611A1 WO 2023044611 A1 WO2023044611 A1 WO 2023044611A1 CN 2021119661 W CN2021119661 W CN 2021119661W WO 2023044611 A1 WO2023044611 A1 WO 2023044611A1
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WO
WIPO (PCT)
Prior art keywords
barrier
box body
urine
collecting
feeding device
Prior art date
Application number
PCT/CN2021/119661
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English (en)
Chinese (zh)
Inventor
景晓源
刘欣安
王立平
Original Assignee
中国科学院深圳先进技术研究院
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Application filed by 中国科学院深圳先进技术研究院 filed Critical 中国科学院深圳先进技术研究院
Priority to PCT/CN2021/119661 priority Critical patent/WO2023044611A1/fr
Publication of WO2023044611A1 publication Critical patent/WO2023044611A1/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K1/00—Housing animals; Equipment therefor
    • A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
    • A01K1/03—Housing for domestic or laboratory animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K1/00—Housing animals; Equipment therefor
    • A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
    • A01K1/035—Devices for use in keeping domestic animals, e.g. fittings in housings or dog beds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements

Definitions

  • the invention relates to a device for collecting excrement of experimental mice.
  • Urine detection in mice is a key physical and chemical indicator. Therefore, collecting the urine of mice is a very critical step.
  • the method of directly collecting animal metabolites with a feces-urine separation device is the metabolic cage method.
  • Chinese utility model patent ZL201310335359.X discloses a simple metabolic cage for mice.
  • This metabolic cage includes a feeding area and a metabolic area. The lower part of the metabolic area is mesh-shaped, and a funnel is connected to it, and a header is received under the funnel.
  • the metabolic cage has a simple structure and low cost, and can separate feces and urine to a certain extent.
  • this kind of metabolic cage requires the operator to put the mice from the feeding area into the metabolic area, and the operator needs to pay attention at all times; the separation of feces and urine is not complete, and the urine in the collection tube is easily mixed with feces; the whole device does not take sealing measures, and the urine in the collection tube The liquid is volatile, resulting in changes in the concentration of urine components.
  • the present invention proposes an experimental mouse excrement collection device, which can effectively separate the feces and urine of the mice.
  • the top of the box body is provided with a cover plate, and the box body is provided with three layers of barriers from top to bottom;
  • the first barrier is placed horizontally, and the space enclosed by it and the box body and cover plate is the mouse placement area;
  • the second barrier and the third barrier are sieve plates, and the second barrier and the third barrier are used to block When the mouse feces fall, the second barrier and the third barrier are set obliquely, and the second barrier and the third barrier can be pulled out from one side of the box;
  • a urine collection funnel is set under the third barrier, and the bottom of the funnel is fixed Urine collection tube, the urine collection tube is connected to the collection bottle;
  • a water feeding device and a feeding device on the outside of the box, and a food trough is fixed in the mouse placement area, and the outlet of the feeding device extends into the trough; the outlet of the water feeding device extends into the mouse from the side wall of the box. placed in the area.
  • a slideway is provided in the above-mentioned box, and the second barrier and the third barrier are respectively put in or drawn out through the slideway.
  • handles are provided on the second barrier and the third barrier to facilitate their extraction from the box.
  • the above-mentioned second barrier and the third barrier are provided with baffles on the side opposite to the handle.
  • one side of the cover plate is hinged to the box body, and a buckle is provided on the side opposite to the hinge, and the cover plate can be fixed to the box body through the buckle.
  • the above-mentioned water feeding device includes a graduated water bottle and a water outlet pipe connected thereto.
  • the end of the water outlet pipe is a water outlet, and the water outlet includes a ball.
  • the diameter of the ball is smaller than the diameter of the water outlet pipe.
  • the side wall at the end of the water outlet pipe is curved. Surface, when the arc surface is in contact with the ball, the surface is completely attached.
  • the above-mentioned feeding device is a funnel, and the feeding outlet at the lower part of the funnel extends into the feeding trough.
  • the side wall of the collection bottle is provided with a scale line
  • the mouth of the collection bottle is provided with a rubber stopper
  • the middle part of the rubber stopper is provided with an inlet.
  • the surface of the above-mentioned urine collection funnel is coated with a lyophobic material.
  • the bottom of the above-mentioned box is provided with a plurality of rollers for easy movement.
  • the mouse placement area of the present invention is equipped with a water feeding device and a feeding device, and is also integrated with a urine and feces separation and collection device. When urine and feces are to be collected, the mice do not need to be transferred;
  • the second barrier and the third barrier of the present invention can be extracted from the box to facilitate the collection of feces on the barrier;
  • the second barrier and the third barrier of the present invention are arranged obliquely, and the feces falling on the barrier will roll down to the lower side of the barrier.
  • the feces on the barrier will not Absorb urine, which is more conducive to complete collection of urine;
  • the surface of the urine collection funnel is coated with a lyophobic material, which is conducive to the complete collection of urine.
  • Fig. 1 is the structural diagram of experimental mouse excreta collecting device of the present invention
  • Fig. 2 is the structural diagram of collection bottle in Fig. 1;
  • Fig. 3 is a structural diagram of the water feeding device in Fig. 1;
  • Fig. 4 is a structural diagram of the second partition in Fig. 1 .
  • the present invention proposes a device for collecting excrement of experimental mice, comprising a box body 1, a cover plate 2 is provided on the top of the box body 1, and three layers of barriers are arranged from top to bottom in the box body 1; the first layer of barriers 3 is placed horizontally , the space surrounded by the box body 1 and the cover plate 2 is a mouse placement area; the second barrier 4 and the third barrier 5 are sieve plates, and the second barrier 4 and the third barrier 5 are used to block small Rat feces fall, the second barrier 4 and the third barrier 5 are arranged obliquely, and the second barrier 4 and the third barrier 5 can be drawn out from the side of the box body 1; the urine collection is arranged under the third barrier 5 Funnel 6, urine collecting tube 7 is fixed below the urine collecting funnel 6, and the urine collecting tube 7 is connected with a collecting bottle 19.
  • Casing body 1 outside is provided with water feeding device 8 and feeding device 9, is fixedly provided with food trough 11 in the mouse placement area, and the food outlet 10 of feeding device 9 stretches in the food trough 11;
  • the side wall of the casing 1 extends into the mouse placement area.
  • the mouse placement area of the present invention is provided with a water feeding device 8 and a feeding device 9, and also integrates a urine and feces separation and collection device. When the urine and feces are to be collected, the mice do not need to be transferred.
  • the water feeding device 8 and the feeding device 9 can be arranged on any side of the casing 1 .
  • the second barrier 4 and the third barrier 5 of the present invention are arranged obliquely, and the feces falling on the barrier will roll down to the lower side of the barrier.
  • the feces on the barrier will not Absorb and influence urine to fall into the urine collection funnel 6, which is more conducive to complete collection of urine.
  • a slideway is provided inside the box body 1, and the second barrier 4 and the third barrier 5 are put in or drawn out through the slideway respectively.
  • the size of the sieve holes on the second barrier 4 and the third barrier 5 can be the same or different, and can prevent the dropping of feces; Diameter of the mesh of barrier 4.
  • the second barrier 4 When extracting the feces of the mice, the second barrier 4 is pulled out, and the mouse feces are picked up with tweezers, and the third barrier 5 does not move. At this time, even if the mice discharge the feces, they will only fall on the third barrier. 5, there is no need to worry about the excrement falling into the urine collection funnel 6. After collecting the feces on the second barrier 4, send the second barrier 4 into the original position along the slideway. If the feces fall on the third barrier 5, the third barrier 5 can be pulled out and picked up with tweezers mouse feces.
  • the box body 1 of the present invention can be made of transparent material, and can also be made of mesh material.
  • the water feeding device 8 and the feeding device 9 can be fixed on the side wall of the casing 1 through the mounting frame.
  • the second barrier 4 and the third barrier 5 are provided with handles 12, which are convenient for the operator to grab and pull out from the box body 1 by hand.
  • the second barrier 4 and the third barrier 5 are provided with baffles 13 on the side opposite to the handle 12 .
  • the baffle plate 13 blocks and collects the falling feces on the second barrier 4 and the third barrier 5 .
  • one side of the cover plate 2 is hinged to the box body 1 , and a buckle 14 is provided on the side opposite to the hinge, and the cover plate 2 can be fixed to the box body 1 through the buckle 14 .
  • the operator When transferring the mice, the operator just unfastens the buckle 14 and flips the cover plate 2 over.
  • the water feeding device 8 includes a graduated water bottle 15 and a water outlet pipe 16 connected thereto.
  • the mouth 17 includes a ball 18, the diameter of which is smaller than that of the water outlet pipe 16, and the side wall at the end of the water outlet pipe 16 is an arc surface, and the surface of the arc surface is completely attached to the ball 18 when it contacts.
  • mice When the mice drink water, they lick the ball 18 to overcome the gravity and water resistance of the ball 18. The ball 18 moves upwards, and the water flows out. When not drinking, the ball 18 rolls down under its own weight. The side wall is fitted to block the water flow.
  • the feeding device 9 is a funnel structure, and the feeding outlet 10 at the lower part of the feeding device 9 protrudes into the feeding trough 11 .
  • the operator quantitatively puts the food into the upper opening of the feeding device 9, and the food slides down. When the food accumulates in the food trough 11, the food no longer slides down. Flow out from the food outlet 10, when avoiding too much food, the mouse will spill the food on the first layer of barrier 3.
  • the side wall of the collection bottle 19 is provided with a scale line, which is convenient for recording the urine amount of the mouse;
  • the mouth of the collection bottle 19 is provided with a rubber stopper 20, and the middle part of the rubber stopper 20 is provided
  • There is an entrance and the urine collection pipe 7 stretches into the middle part of the rubber plug 20 and is provided with the entrance to play a sealing role, and the rubber plug 20 prevents the volatilization of urine.
  • the surface of the urine collection funnel 6 is coated with a lyophobic material to prevent urine from stagnating on the surface of the urine collection funnel 6, reduce urine loss, and collect precisely.
  • the limit portion of urine collection funnel 6 is fixed on the inwall of casing 1, and collection bottle 19 can be fixed with fixture.
  • a plurality of rollers 21 are provided at the bottom of the box body 1 to facilitate the operator to move the collecting device.

Abstract

L'invention concerne un dispositif de collecte d'excréments de souris de laboratoire comprenant un corps de boîte (1). Une plaque de recouvrement (2) est disposée au niveau de la partie inférieure du corps de boîte (1), et trois couches de cloison sont disposées dans le corps de boîte (1) de haut en bas; une première couche de cloison (3) est placée horizontalement, et un espace défini par la première couche de cloison (3) conjointement avec le corps de boîte (1) et la plaque de recouvrement (2) est une zone de placement de souris; une seconde cloison (4) et une troisième cloison (5) sont des plaques de tamis, la seconde cloison (4) et la troisième cloison (5) sont utilisées pour bloquer la chute des excréments de souris, la seconde cloison (4) et la troisième cloison (5) sont disposées de manière oblique, et la seconde cloison (4) et la troisième cloison (5) peuvent être retirées d'un côté du corps de boîte (1); un entonnoir de collecte d'urine (6) est disposé en dessous de la troisième cloison (5), un tuyau de collecte d'urine (7) est fixé au-dessous de l'entonnoir de collecte d'urine (6), et le tuyau de collecte d'urine (7) est relié à un récipient de collecte (19).
PCT/CN2021/119661 2021-09-22 2021-09-22 Dispositif de collecte d'excréments de souris de laboratoire WO2023044611A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/CN2021/119661 WO2023044611A1 (fr) 2021-09-22 2021-09-22 Dispositif de collecte d'excréments de souris de laboratoire

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2021/119661 WO2023044611A1 (fr) 2021-09-22 2021-09-22 Dispositif de collecte d'excréments de souris de laboratoire

Publications (1)

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WO2023044611A1 true WO2023044611A1 (fr) 2023-03-30

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN202663932U (zh) * 2012-06-26 2013-01-16 南方医科大学 一种改进型实验鼠代谢笼
CN203775876U (zh) * 2013-12-24 2014-08-20 苏州大学 一种大小鼠代谢笼
CN206323949U (zh) * 2016-12-22 2017-07-14 上海中医药大学附属岳阳中西医结合医院 一种用于收集无菌尿液的实验小鼠代谢笼
CN209185349U (zh) * 2018-10-24 2019-08-02 贵州医科大学 一种小鼠代谢笼
US20200060236A1 (en) * 2016-06-29 2020-02-27 Riken System for restraining mouse
CN112602601A (zh) * 2020-12-23 2021-04-06 中国科学院亚热带农业生态研究所 一种山羊精准饲喂及代谢自动化监测的呼吸代谢笼

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN202663932U (zh) * 2012-06-26 2013-01-16 南方医科大学 一种改进型实验鼠代谢笼
CN203775876U (zh) * 2013-12-24 2014-08-20 苏州大学 一种大小鼠代谢笼
US20200060236A1 (en) * 2016-06-29 2020-02-27 Riken System for restraining mouse
CN206323949U (zh) * 2016-12-22 2017-07-14 上海中医药大学附属岳阳中西医结合医院 一种用于收集无菌尿液的实验小鼠代谢笼
CN209185349U (zh) * 2018-10-24 2019-08-02 贵州医科大学 一种小鼠代谢笼
CN112602601A (zh) * 2020-12-23 2021-04-06 中国科学院亚热带农业生态研究所 一种山羊精准饲喂及代谢自动化监测的呼吸代谢笼

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