WO2023044550A1

WO2023044550A1 - Method for acting upon plants using sequences of extremely low frequencies and guide frequencies

Info

Publication number: WO2023044550A1
Application number: PCT/BR2022/050376
Authority: WO
Inventors: Luzo DANTAS JUNIOR
Original assignee: Dantas Junior Luzo
Priority date: 2021-09-27
Filing date: 2022-09-25
Publication date: 2023-03-30
Also published as: BR102021019311A2; AU2022351991A1

Abstract

A method is disclosed for acting upon chemical elements and combinations thereof, including plant proteins, DNA, RNA, hormones and neurotransmitters, by transmitting target-defined sequences of extremely low frequencies and sets of specific guide elements that will act upon given plant amino acid and/or nucleotide sequences, inter alia. The intended effect is achieved by strengthening or weakening the bonds between the chemical elements and combinations thereof, including the elements that make up the plant nucleotides, amino acids, hormones and neurotransmitters, inter alia.

Description

PROCESS FOR ACTIVATION IN PLANTS THROUGH EXTREMELY LOW FREQUENCY SEQUENCES AND FREQUENCIES

GUIDELINES

DESCRIPTION REPORT

FIELD OF THE INVENTION

[0001] Process for acting on chemical elements and their combinations, including proteins, DNA, RNA, hormones and neurotransmitters from plants through the transmission of extremely low frequency sequences defined from targets and a set of specific drivers that will act on certain sequences of amino acids and/or nucleotides, among others, from plants. The desired effect is achieved by strengthening or attenuating the bonds between chemical elements and their combinations, including constituent elements of nucleotides, amino acids, hormones and neurotransmitters, among others, related to plants.

[0002] In general, the degree of variety of species and genetic mutations of plants makes it quite complex and costly to define a solution for eliminating or reducing their impact on crop productivity. There are currently two most common types of solutions available for controlling plant growth: chemical and biological. Both have been used, but can have undesirable effects such as various side effects in humans, leaving residues in food of plant and animal origin (with the potential to cause disease in humans and animals), contamination of soils, rivers, lakes and water tables, intoxication of professionals who apply the products in the field, among others. In addition, they may have low efficiency in the face of frequent plant mutations and high selection pressure for populations resistant to chemical products. [0003] The invention filed on 10/30/2018, under number BR102017023442-8 and entitled "Product and Process for Generating Extremely Low Frequencies for Chemical Equalization" describes a process and product for chemical equalization, based on the transmission of frequencies that will act in the equalization of chemical elements or a substance, providing, consequently, the adjustment of strengthening or attenuation of its properties and physical-chemical characteristics as desired, improving quality, increasing productivity and efficiency of industrial processes.

[0004] By strengthening the physical-chemical properties and characteristics of an element, it is possible to use it in significantly smaller dosages, without, however, impacting the results obtained. This invention, however, does not have the structure of directors, capable of guiding the frequency sequences to specific targets, nor the ability to act on biological organisms.

[0005] The invention BR 10 2019 025958 2 - PRODUCT AND PROCESS FOR ACTING IN BIOLOGICAL ORGANISMS THROUGH EXTREMELY LOW FREQUENCIES of 12/07/2019 reveals a product and process for the use of extremely low frequency sequences for use in organisms. However, said invention does not define specific processes for the use of this procedure in the case of vegetables.

[0006] The invention US20190223387 - LIGHT CONTROL DEVICE FOR STIMULATING PLANT GROWTH AND METHOD THEREOF of 01/24/2019 discloses a light control device and method for stimulating plant growth. The light control device includes a light source capable of emitting light necessary for plant growth. The light control device further comprises a plurality of light converting films rotatably mounted on a film switching unit disposed around the light source, the film switching unit driven by a motor disposed therein. of light conversion films can still provide a plurality of light spectra required during a plurality of plant growth stages. The light control device determines the growth stage of plants based on the size and height of the plants. The motor rotates the film switching unit, based on the growth stage of the plants, in such a way that a light converting film associated with the growth stage of the plants is positioned in front of the light source. Such placement stimulates plant growth. However, said invention does not deal with the use of Extremely Low Frequency (ELF) sequences in the range from 0 to 100Hz, and does not specify the molecular targets of plants that are reached by the frequencies transferred through light.

[0007] The invention US3579081A - LOW FREQUENCY SINE WAVE GENERATOR CIRCUIT of 12/11/1968 discloses a sinusoidal signal generator circuit, characterized by the fact that it comprises at least two bridge circuits, each comprising two pairs of control means of bidirectional current connected in series connected across a direct current DC voltage source, each bridge circuit having a pair of output terminals at the junction of the current control means said pairs of current control means at very low frequencies, as 60 cycles per second, being connected directly in series. The current control means allow to produce different rectangular pulse output waveforms at the output terminals of said bridge circuit, each waveform respectively comprising positive and negative pulses of the same amplitude with respect to zero. However, said invention does not deal with the use of Extremely Low Frequency (ELF) sequences in the range of 0 to 100Hz to act on plants and does not specify molecular targets of plants that are reached by the frequencies produced by the device.

[0008] The invention EP2753173 - HERBICIDAL COMPOSITION COMPRISING POLYMERIC MICROPARTICLES CONTAINING A HERBICIDE of 09/13/2012 discloses a berbinoid composition comprising a mixture of: (a) polymeric microparticles containing a first herbicide, in which the first herbicide is an auxin herbicide synthetic (e.g., dicamba, MCPA, or 2,4-D) or an acetolactate synthase (ALS) inhibitor herbicide (e.g., triaulfuron, tribenuron-methyl, iodosulfuron-methyl, mesosulfuron-methyl, sulfosulfuron, flupirulon-methyl, or piroxsulam); wherein the first herbicide, when in a salt-free form and when not contained within polymeric microparticles, antagonizes the herbicidal activity of pinoxaden; and (b) pinoxaden; wherein the polymeric microparticles are controlled release matrices within which is the first herbicide and which function to control and/or delay the release of the first herbicide from the polymeric microparticles into a liquid (e.g. aqueous) medium when the polymeric microparticles are placed (e.g., dispersed) in contact with the liquid medium, it is believed that containment of the first herbicide within the controlled release polymeric microparticles mitigates the antagonism of pinoxaden's grass-weed herbicidal activity that may otherwise , be caused by the first herbicide, depending on the circumstances. The invention also provides a method of reducing the antagonistic effect on monocotyledonous weed control in non-oat cereals which is shown by a herbicidal mixture of a synthetic auxin herbicide with a pinoxaden inhibitor herbicide or an ALS inhibitor with pinoxaden, which comprises applying a herbicidal composition according to the invention. The invention also provides a herbicidal composition comprising (a) polymeric microparticles (e.g. controlled release matrices), containing a first herbicide as defined above, and: (x) a non-ionic surfactant or (y) a surface-modified clay, as defined here. However, said invention does not deal with the use of Extremely Low Frequency (ELF) sequences in the range from 0 to 100Hz for the purpose of herbicidal action, chemical molecules being used for this purpose. GENERAL DESCRIPTION

[0009] Due to the gaps found in the state of the art of using extremely low frequency sequences to deal specifically with plants, it was necessary to develop a new technique as shown below. In the case of plants, we can predict up to three main attack strategies:

[0010] 1 . Act on specific sequences of amino acids that make up specific sites of plant proteins. In this case, the aim is to alter the natural biological function of the target proteins with the aim of stimulating development and/or inhibiting development and/or modifying the plant's defense mechanisms.

[0011] 2. Act on nucleotide sequences in order to inhibit or stimulate the gene expression of important proteins for essential physiological processes for life, multiplication and/or defense of plants.

[0012] 3. Act on elements and other components of plants, such as hormones, lipids and neurotransmitters, among others based on chemical elements and/or specific chemical compounds and/or their combinations for each plant or group of plants, including their variations and/ or mutations, in order to stimulate or inhibit, totally or partially, its biological function. All these action strategies use the same technique used here.

[0013] The objective of the technique demonstrated here is to inhibit or stimulate biological functions or modify the properties of elements, or chemical compounds, defined in the attack strategies listed above through the application of sequences of extremely low frequencies and a set of predefined driving frequencies .

[0014] The form of action developed and revealed in this technique is based on the application of the technique of sequences of extremely low frequencies, that is, frequencies in the open interval between 0 and 100 Hertz (limits not included). Each set of frequency sequences is composed of one or more targets and one or more drivers specific to each plant species and/or its variations and mutations. This makes this plant-specific and application-specific set of functions biological and/or physiological properties chosen in them. This invention discloses such application processes which are illustrated in the figure. Each target and driver combination is designed to define a mathematically unique solution.

[0015] In case 1, above, this invention makes use of one or more previously determined frequency sequences (each with a target and driver determining a single solution) and validated in the laboratory, to generate a decrease or increase in the interatomic distance of chemical elements that form the target amino acids, altering the conformation of the protein in question and consequently its biological function.

[0016] In case 2, above, this invention makes use of one or more previously determined frequency sequences (each with a target and driver determining a single solution) and validated in the laboratory, to generate a decrease or increase in the interatomic distance of chemical elements that form the target nucleotides, altering the conformation of these nucleotides and consequently their biological function.

[0017] In case 3, above, this invention makes use of one or more previously determined frequency sequences (each with a target and driver determining a single solution) and validated in the laboratory, to generate a decrease or increase in the interatomic distance of chemical elements and/or chemical compounds (such as hormones, lipids and neurotransmitters, among others) target, altering their conformation and consequently their properties and/or biological functions.

[0018] The three strategies described above can be used individually and/or concomitantly in the same implementation of the technique disclosed here.

PREFERRED MODALITIES

[0019] A first preferred modality deals with the application of strategy 1 (acting on amino acids) which targets the amino acid sequences of the enzyme acetyl-coenzyme-A Carboxylase (ACCase) of plants. In that case the sequences of frequencies will act according to their directing frequencies on the amino acid sequences that form the different possibilities of active site (binding site) of the enzyme acetyl-coenzyme-A Carboxylase (ACCase) of plants. As a consequence of this action, the action of the enzyme acetyl-coenzyme-A Carboxylase (ACCase) of plants will be stimulated, prevented or hindered, causing stimulation or inhibition of the synthesis of fatty acids, which are constituents of lipids that occur in the membranes of cells and organelles of the plants.

[0020] A second preferred modality deals with the application of strategy 1 (acting on amino acids) which targets the amino acid sequences of the enzyme acetolactate synthase (ALS) in plants (Figure 2). In this case, the frequency sequences will act according to their directing frequencies on the amino acid sequences that form the different possibilities of active site (binding site) of the enzyme acetolactate synthase (ALS) in plants. As a consequence of this action, the action of the enzyme acetolactate synthase (ALS) in plants will be stimulated, prevented or hindered, causing stimulation or inhibition of the biosynthesis of the branched-chain aliphatic amino acids leucine (Leu), valine (Vai) and isoleucine (lie) of plants.

[0021] A third preferred modality deals with the application of strategy 3 (acting on hormone) which targets the Auxin hormone of plants. In this case, the frequency sequences will act according to their directing frequencies on the chemical sequence that form the different possibilities of the active site of the Auxin hormone in plants. As a consequence of this action, the biological function of the Auxin hormone in the plants will be stimulated, prevented or hindered, causing stimulation or inhibition in the growth of the plants.

[0022] A fourth preferred modality deals with the application of strategy 1 (acting on amino acids) which targets the amino acid sequences of the enzymes that participate in the biosynthesis of carotenoids in plants. In this case, the frequency sequences will act according to their directing frequencies on the amino acid sequences that form the different possibilities of active site (binding site) of the enzymes of the enzymes that participate in the biosynthesis of plant carotenoids. As a consequence of this action, the action of the enzymes that participate in the biosynthesis of carotenoids will be stimulated, prevented or hindered, causing stimulation or inhibition of the photodegradation of chlorophyll.

[0023] A fifth preferred modality deals with the application of strategy 1 (acting on amino acids) which targets the amino acid sequences of the protein called tubulin in plants. In this case, the frequency sequences will act according to their directing frequencies on the amino acid sequences that form the different possibilities of active site (binding site) of plant tubulin. As a consequence of this action, the action of tubulin in plants will be stimulated, prevented or hindered, causing stimulation or inhibition of tubulin polymerization, stimulating or hindering plant cell division (mitosis) [0024] A sixth preferred modality deals with the application of the strategy 1 (acting on amino acids) which targets the amino acid sequences (figure 3) of the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPs) in plants. In this case, the frequency sequences will act according to their directing frequencies on the amino acid sequences that form the different possibilities of active site (binding site) of the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPs) of plants. As a consequence of this action, the action of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPs) in plants will be stimulated, prevented or hindered, causing stimulation or inhibition of the synthesis of essential aromatic amino acids phenylalanine, tyrosine and tryptophan, which are precursors of other products , such as lignin, alkaloids, flavonoids and benzoic acids, essential for plant development.

[0025] A seventh preferred modality deals with the application of strategy 1 and 3 together or separately (1: action on amino acids; 3: action on chemical elements and/or chemical compounds) which targets the binding of the chemical molecule plastoquinone to protein D1 in Photosystem II of plants. In this case, the sequences of frequencies will act according to their driving frequencies on the chemical sequences that form the plastoquinone molecule and on the chemical sequences that form the different possibilities of active site (binding site) of protein D1 in Photosystem II of plants. As a consequence of this action, the binding of the chemical molecule plastoquinone to protein D1 in Photosystem II of plants will be stimulated or inhibited, causing inhibition or stimulation of the flow of electrons in Photosystem II. In the case of inhibition of the connection between plastoquinone and D1 protein, the formation of free radicals consequently occurs, which generate peroxidation of lipids in the cell membrane and cell death. In the case of stimulation, acceleration of the electron transfer process occurs, generating a higher rate of photosynthesis. [0026] An eighth preferred modality deals with the application of strategy 1 (acting on amino acids) which targets the amino acid sequences of the enzyme glutamine synthetase (GS) of plants (Figure 4). In this case, the frequency sequences will act according to their directing frequencies on the amino acid sequences that form the different possibilities of active site (binding site) of the enzyme glutamine synthetase (GS) in plants. As a consequence of this action, the action of the enzyme glutamine synthetase (GS) in plants will be stimulated, prevented or hindered, causing stimulation or inhibition of its biological action in the pathways of nitrogen and ammonium assimilation, amino acid synthesis, photorespiration and prevention of inhibition. of the enzyme ribulose-1, 5-biophosphate carboxylase (RUBISCO), essential for carbon fixation. Inhibition of the glutamine synthetase (GS) enzyme in plants results in ammonium accumulation and subsequent plant death.

[0027] A ninth preferred modality deals with the application of strategy 1 (acting on amino acids) which targets the amino acid sequences of the enzyme protoporphyrinogen oxidase (PROTOX) of plants. In this case, the frequency sequences will act according to their directing frequencies on the amino acid sequences that form the different possibilities of active site (binding site) of the enzyme protoporphyrinogen oxidase (PROTOX) of plants. As a consequence of this action, the action of the protoporate enzyme will be stimulated, prevented or hindered. firinogen oxidase (PROTOX) of plants, causing stimulation or inhibition of its biological action of protoporphyrinogen oxidation in protoporphyrin IX, which is a precursor of chlorophyll and heme groups (necessary for electron transfer). Inhibition of the enzyme protoporphyrinogen oxidase (PROTOX) in plants results in oxidation of lipids and proteins in the cell cytoplasm and consequent plant death.

[0028] A tenth preferred modality deals with the application of strategy 1 (acting on amino acids) which targets the amino acid sequences, in whole or in part, that make up the proteins of the cellular respiration complex. In this case, the frequency sequences will act according to their directing frequencies on the amino acid sequences that form the active site (binding site) of the aforementioned proteins. As a consequence of this action, cellular respiration of plants will be inhibited, impeded or hindered, totally or partially.

[0029] An eleventh preferred modality deals with the application of strategy 1 (acting on amino acids) which targets the amino acid sequences, in whole or in part, that make up the proteins that act in the synthesis of amino acids and proteins. In this case, the frequency sequences will act according to their directing frequencies on the amino acid sequences that form the active site (binding site) of the proteins that act in the synthesis of amino acids and plant proteins. As a consequence of this action, the synthesis of amino acids and plant proteins will be prevented or hindered.

Claims

1 . Process for acting on plants by means of sequences of extremely low frequencies and driving frequencies characterized by making use of sequences of frequencies that are unique for each target determined by the driving frequencies in one or more plants (including their variations and/or mutations), which act on specific amino acid sequences, stimulating or inhibiting, totally or partially, their biological function.

2. Process according to claim 1, characterized by targeting the amino acid sequences, in whole or in part, that make up the proteins of the cellular respiration complex, stimulating or inhibiting, totally or partially, cellular respiration in plants.

3. Process according to claim 1, characterized in that it targets the amino acid sequences, in whole or in part, that make up the proteins of the photosystem complex I and II, stimulating or inhibiting, totally or partially, photosynthesis in plants.

4. Process according to claim 1 characterized by targeting the amino acid sequences, in whole or in part, that make up the proteins that act in the synthesis of amino acids and proteins, stimulating or inhibiting, totally or partially, the synthesis of amino acids and proteins in plants .

5. Process for acting on plants through sequences of extremely low frequencies and driving frequencies characterized by making use of sequences of frequencies that are unique for each target determined by the driving frequencies in one or more plants (including their variations and/or mutations) , which act on certain nucleotide sequences, stimulating or inhibiting, totally or partially, their biological function.

6. Process according to claim 5 characterized by targeting nucleotide sequences, in whole or in part, that can be transcribed and translated into proteins that act in vital functions to plants, stimulating or inhibiting, totally or partially, the synthesis of amino acids, peptides and proteins from plants.

7. Process according to claim 5 characterized by targeting nucleotide sequences, in whole or in part, that can be transcribed and translated into proteins that act in inducing plant defense, improving the induction of their defense mechanisms against parasites and/or disease-causing microorganisms.

8. Process for acting on plants through sequences of extremely low frequencies and driving frequencies characterized by making use of sequences of frequencies that are unique for each target determined by the driving frequencies in one or more plants (including their variations and/or mutations) , to act on chemical elements and/or chemical compounds, such as hormones, lipids and neurotransmitters, among others, stimulating or inhibiting, totally or partially, their biological function.

9. Process according to claim 8 characterized by targeting chemical elements and/or chemical compounds, in whole or in part, that make up the hormones that act in plant growth and development processes, such as the hormone auxin, cytokinin and gibberellin, stimulating or inhibiting, totally or partially, the action of the hormone auxin and/or cytokinin and/or gibberellin in plants.

10. Process according to claim 8 characterized by targeting the chlorophyll molecule, in whole or in part, which acts in the process of photosynthesis in plants, stimulating or inhibiting, totally or partially, the action of chlorophyll in plants.