WO2023025333A1 - 体外分析诊断检测装置及pcr模组 - Google Patents

体外分析诊断检测装置及pcr模组 Download PDF

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Publication number
WO2023025333A1
WO2023025333A1 PCT/CN2022/127913 CN2022127913W WO2023025333A1 WO 2023025333 A1 WO2023025333 A1 WO 2023025333A1 CN 2022127913 W CN2022127913 W CN 2022127913W WO 2023025333 A1 WO2023025333 A1 WO 2023025333A1
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WIPO (PCT)
Prior art keywords
heat conduction
plate
reagent cartridge
pcr
bracket
Prior art date
Application number
PCT/CN2022/127913
Other languages
English (en)
French (fr)
Inventor
钮烨
梅哲
何志平
朱志华
苏志江
张彤
Original Assignee
广州万孚生物技术股份有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Priority claimed from CN202111264219.9A external-priority patent/CN115725404A/zh
Priority claimed from CN202111264176.4A external-priority patent/CN115725403A/zh
Priority claimed from CN202111264160.3A external-priority patent/CN115722287A/zh
Priority claimed from CN202111264188.7A external-priority patent/CN115877025A/zh
Application filed by 广州万孚生物技术股份有限公司 filed Critical 广州万孚生物技术股份有限公司
Publication of WO2023025333A1 publication Critical patent/WO2023025333A1/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/02—Apparatus for enzymology or microbiology with agitation means; with heat exchange means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C12M1/38—Temperature-responsive control
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing

Definitions

  • the present disclosure relates to the technical field of in vitro analysis and diagnosis, in particular to an in vitro analysis and diagnosis detection device and a PCR module.
  • Pathogen POCT point-of-care testing, timely detection
  • the instrument system is suitable for analyzing nasopharyngeal swabs, sputum, urine fluid, feces, cervical/vaginal swabs, blood, cerebrospinal fluid, skin, wound swabs and other sample types, add body fluid samples to the disposable reagent cartridge, put the cartridge into the instrument system, and undergo nucleic acid amplification , detection, calculation and result printout, the entire analysis process is fully automated, and personnel without professional training can easily use it, and obtain reliable and accurate detection results.
  • the instruments and reagents are suitable for single or multiple detection of DNA or RNA of pathogens that infect the human body.
  • the centrifugal column method or magnetic bead method used in the field of biotechnology for nucleic acid extraction generally requires four steps of lysis, binding, cleaning, and elution, plus subsequent nucleic acid molecular hybridization, polymerase chain reaction, and molecular detection. step.
  • Molecular detection technology is developing in the direction of accuracy, convenience, sensitivity, automation and integration.
  • due to the technical complexity of molecular detection there are very few fully automated instrument platforms from sample to result, and the structure for heating and cooling the sample liquid in the PCR chamber is relatively complicated, and the heating and cooling effect is not ideal.
  • a PCR module the PCR module includes: a first bracket, the first bracket is used to install the reagent cartridge; A substrate on a bracket, a semiconductor refrigerator arranged on the substrate, and a heat conduction plate arranged on the semiconductor refrigerator, the heat conduction plate is used for connecting with one side of the PCR chamber of the reagent cartridge Tight interference; window assembly, the window assembly includes an optical window, the optical window is provided with at least one transparent plate, and the transparent plate is used for tight interference with the other side of the PCR chamber.
  • An in vitro analysis and diagnosis detection device includes the PCR module.
  • the reagent cartridge when working, the reagent cartridge is installed on the first bracket, so that the heat conduction plate is in close contact with one side of the PCR chamber, and the transparent plate is in contact with the other side of the PCR chamber. One side is in close contact with each other, and then the semiconductor refrigerator performs cyclic heating and cooling actions.
  • the semiconductor refrigerator When heating up, the heat is transferred to the PCR chamber through the heat conduction plate, and when the temperature is lowered, the heat is transferred from the PCR chamber to the substrate through the heat conduction plate, and then to the heat sink.
  • the heat is transferred from the heat sink to the external environment, thereby cooling the PCR chamber, and at the same time, the excitation light from the optical detection module is injected into the sample liquid in the PCR chamber through the transparent plate of the optical window, and the sample liquid generates fluorescence and reflects back to the optical detection
  • the module performs optical detection, which can quantitatively detect the number of samples amplified in each thermal cycle.
  • the overall structure of the PCR module is relatively simple.
  • FIG. 1 is a structural schematic view of a viewing angle of a PCR module according to an embodiment of the present disclosure
  • Fig. 2 is a structural schematic view of one of the viewing angles in which the window assembly and the optical detection module are hidden in Fig. 1;
  • Fig. 3 is a structural schematic diagram of another viewing angle with the window assembly and the optical detection module hidden in Fig. 1;
  • FIG. 4 is a schematic structural diagram of a reagent cartridge according to an embodiment of the present disclosure.
  • FIG. 5 is a structural diagram of a viewing angle of a heating and cooling component of a PCR module according to an embodiment of the present disclosure
  • FIG. 6 is another perspective structure diagram of the heating and cooling component of the PCR module according to an embodiment of the present disclosure
  • Fig. 7 is a schematic cross-sectional structural view at A-A of Fig. 6;
  • FIG. 8 is a structural schematic view of one view of the window assembly of the PCR module installed on the second mounting board according to an embodiment of the present disclosure
  • FIG. 9 is a structural schematic diagram of another viewing angle in which the window assembly of the PCR module is installed on the second mounting plate according to an embodiment of the present disclosure.
  • Fig. 10 is a schematic diagram of the enlarged structure at B in Fig. 9;
  • Fig. 11 is a schematic cross-sectional structural view at C-C of Fig. 10;
  • Fig. 12 is a structural view of a first mounting board according to an embodiment of the present disclosure.
  • Fig. 13 is another perspective structure diagram of the first mounting board according to an embodiment of the present disclosure.
  • Fig. 14 is another perspective structure diagram of the first mounting board according to an embodiment of the present disclosure.
  • Figure 15 is a sectional view at D-D of Figure 14;
  • FIG. 16 is a structural view of a slide rail assembly according to an embodiment of the present disclosure.
  • Fig. 17 is another perspective structure diagram of the slide rail assembly according to an embodiment of the present disclosure.
  • Fig. 18 is another perspective structural view of the first bracket according to an embodiment of the present disclosure.
  • Fig. 19 is a schematic diagram of an enlarged structure at m after the guide plate in the structure shown in Fig. 18 is hidden;
  • FIG. 20 is a structural schematic diagram of a viewing angle of a temperature regulating member according to an embodiment of the present disclosure
  • Fig. 21 is a schematic cross-sectional structure diagram at n-n of Fig. 20;
  • Fig. 22 is a structural schematic diagram of another viewing angle of a temperature regulating member according to an embodiment of the present disclosure.
  • Fig. 23 is a schematic structural view of a first bracket provided with a temperature regulating member according to an embodiment of the present disclosure
  • Fig. 24 is a schematic cross-sectional structural view at p-p of Fig. 23;
  • FIG. 25 is a structural schematic view of one viewing angle of an optical detection module according to an embodiment of the present disclosure.
  • Fig. 26 is a structural schematic diagram of another viewing angle of Fig. 25;
  • Fig. 27 is a structural view of one of the perspectives in which the moving mechanism is hidden in Fig. 25;
  • Fig. 28 is another perspective structure diagram with the moving mechanism hidden in Fig. 25;
  • Figure 29 is a sectional view at Q-Q of Figure 28;
  • FIG. 30 is a perspective structural diagram of a moving mechanism according to an embodiment of the present disclosure.
  • Fig. 31 is another structural view of the moving mechanism according to an embodiment of the present disclosure.
  • First bracket 11. Support plate; 111. Second recess; 112. Import and export; 113. Third recess; 114. First installation hole; 115. Activity room; 12. Guide plate; 121. First pass Hole; 122, second via hole; 16, limit baffle; 161, blocking part; 1611, guide edge; 162, rotating member; 17, sixth elastic member; 20, heating and cooling assembly; 21, substrate; Semiconductor refrigerator; 23, heat conducting plate; 24, heat dissipation member; 25, first positioning plate; 30, window assembly; 31, optical window; 311, transparent plate; 312, PC board; 32, first elastic member; 33, The first carrier plate; 34, the hollow opening; 40, the reagent cartridge; 41, the first air hole; 42, the second air hole; 44, the first valve; 45, the second valve; 46, the sample chamber; 47, pretreatment chamber; 48, mixing chamber; 49, PCR chamber; 50, first pressing assembly; 51, first driving mechanism; 52, first air pipe; 521, first joint; 522, second joint; 523, 53, the second air air
  • FIG. 1 shows a schematic structural view of a PCR module according to an embodiment of the present disclosure
  • FIG. 2 and FIG. 3 show that the window assembly 30 and the optical detection module 100 are hidden
  • FIG. Fig. 4 shows a schematic structural diagram of a reagent cartridge 40 according to an embodiment of the present disclosure
  • Fig. 5 and Fig. 6 respectively show two differences of the heating and cooling assembly 20 according to an embodiment of the present disclosure.
  • FIG. 7 shows a schematic cross-sectional structural diagram at A-A of FIG. .
  • An embodiment of the present disclosure provides a PCR module.
  • the PCR module includes a first bracket 10 , a heating and cooling component 20 and a window component 30 .
  • the first bracket 10 is used for installing the reagent cartridge 40 .
  • the heating and cooling assembly 20 includes a substrate 21 disposed on the first bracket 10 , a semiconductor cooler 22 disposed on the substrate 21 , and a heat conducting plate 23 disposed on the semiconductor cooler 22 .
  • the heat conduction plate 23 is used to tightly contact with one side of the PCR chamber 49 of the reagent cartridge 40 .
  • the window assembly 30 includes an optical window 31 .
  • the optical window 31 is provided with at least one transparent plate 311 .
  • the transparent plate 311 is used to closely interfere with the other side of the PCR chamber 49 .
  • the reagent cartridge 40 is installed on the first bracket 10, so that the heat conducting plate 23 is in close contact with one side of the PCR chamber 49, and the transparent plate 311 is in contact with the other side of the PCR chamber 49.
  • the sides are in close contact with each other, and then the semiconductor refrigerator 22 performs cyclic heating and cooling actions.
  • the temperature is raised, the heat is transferred to the PCR chamber 49 through the heat conduction plate 23, and when the temperature is lowered, the heat is transferred from the PCR chamber 49 to the substrate 21 through the heat conduction plate 23.
  • the heat is transferred to the heat sink, and the heat is transferred to the external environment by the heat sink, so that the PCR chamber 49 is cooled.
  • the excitation light from the optical detection module 100 is injected into the sample liquid in the PCR chamber 49 through the transparent plate 311 of the optical window 31.
  • the sample liquid generates fluorescence and reflects it back to the optical detection module 100 for optical detection, and can perform quantitative detection of the sample amplification quantity in each thermal cycle.
  • the PCR chamber 49 of the reagent cartridge 40 has a certain pressure and slightly bulges, after being compressed by the optical window 31, the other side of the PCR chamber 49 has a good contact with the heat conducting plate 23, thereby greatly reducing the material interface.
  • the overall structure of the PCR module is relatively simple.
  • the reagent cartridge 40 in this embodiment is provided with at least two chambers, the circulation path, the first air hole 41 and the second air hole 42, at least two chambers communicate through the flow path, the first air hole 41,
  • the second air holes 42 are all in communication with the circulation passage, and the specific structural form of the circulation passage is not limited here, and can be set according to actual needs.
  • the at least two chambers include, for example, a sample chamber 46 , a pretreatment chamber 47 , a mixing chamber 48 and a PCR chamber 49 , and of course other functional chambers may also be used.
  • the specific numbers of the sample chamber 46 , the pretreatment chamber 47 , the mixing chamber 48 and the PCR chamber 49 are not limited, and can be set according to actual needs.
  • sample chamber 46 which is used to install the sample liquid
  • pretreatment chamber 47 which is used to crack the sample liquid, for example, by heating
  • mixing chambers 48 for example, are used to make the sample liquid
  • the liquid is mixed with the freeze-dried reagent
  • PCR chambers 49 which are used to perform polymerase chain reaction and molecular detection on the sample liquid.
  • the number of transparent plates 311 of the optical window 31 can be one, two, three or other numbers, which is not limited here, and is specifically set according to the PCR chamber 49 of the reagent cartridge 40, and the PCR The chambers 49 are provided in one-to-one correspondence.
  • the optical window 31 includes a PC board 312 , and the transparent board 311 is fixed on the PC board 312 by, for example, screwing, bonding, ultrasonic welding or laser welding. It should be noted that the transparent board 311 is specifically selected from optical materials, which may be the same as or different from the material of the PC board 312 , which is not limited here.
  • Figure 9 shows a structural schematic diagram of another viewing angle in which the window assembly 30 of the PCR module according to an embodiment of the present disclosure is installed on the second mounting plate 63
  • Figure 10 shows the structure at B in Figure 9
  • Figure 11 shows a schematic diagram of the cross-sectional structure of Figure 10 at C-C.
  • the heating and cooling assembly 20 further includes a heat sink 24 .
  • the heat sink 24 is connected to the substrate 21 .
  • the heat dissipation element 24 includes a heat dissipation plate stacked on the base plate 21, and a plurality of heat dissipation fins connected to the heat dissipation plate.
  • the heat sink 24 when the semiconductor refrigerator 22 is used to heat up the PCR chamber 49, the heat sink 24 will dissipate the cold better through the substrate 21; when the semiconductor refrigerator 22 is used to cool down the PCR chamber 49, The heat sink 24 dissipates the heat to the outside better through the base plate 21 .
  • the PCR module can also be provided with a fan or a water cooling structure, and the airflow blown to the heat sink 24 by the fan can quickly take away the heat of the heat sink 24, or contact the heat sink 24 through the water cooling structure Quickly take away the heat of the radiator 24.
  • the heating and cooling assembly 20 further includes a first positioning plate 25, and the first positioning plate 25 is fixedly installed on the base plate 21 through at least one mounting member.
  • the first positioning plate 25 is provided with a first opening
  • the semiconductor refrigerator 22 and the heat conduction plate 23 are both arranged in the first opening
  • the edge of the first opening and the outer edge of the heat conduction plate 23 are clamped, bonded, or Fixedly connected with mounting parts.
  • the PCR module further includes a first pressing component 50 .
  • the first pressing assembly 50 includes a first driving mechanism 51 , a first mounting plate 54 and at least one first pressing member 56 .
  • the first driving mechanism 51 is disposed on the first bracket 10 , and the first driving mechanism 51 is connected to the first mounting plate 54 for driving the first mounting plate 54 to move toward or away from the reagent cartridge 40 .
  • At least one first pressing member 56 is disposed on the first mounting plate 54 , and the first pressing member 56 is used for pressing or releasing the first valve 44 of the reagent cartridge 40 .
  • the first pressing member 56 is, for example, a thimble, a push rod, etc., as long as the first valve 44 can be pushed to close the first valve 44, or the first valve 44 can be opened when the first valve 44 is released.
  • the size of the conflicting end surface of the first pressing member 56 is set corresponding to the size of the first valve 44 .
  • the first compression assembly 50 further includes a first air tube 52 and a second air tube 53 .
  • the first air pipe 52 and the second air pipe 53 are all arranged on the first mounting plate 54.
  • One end of the first air pipe 52 is provided with a first joint 521, and the other end of the first air pipe 52 is provided with a second joint 522.
  • the second air pipe 53 One end of the second air pipe 53 is provided with a third joint 531, and the other end of the second air pipe 53 is provided with a fourth joint 532.
  • the first driving mechanism 51 drives the first mounting plate 54 to move to the first position
  • the first joint 521 and the third joint 531 are respectively used for docking communication with the first air hole 41 and the second air hole 42 of the reagent cartridge 40 .
  • the PCR module also includes a gas circuit control component (not shown in the figure) and a pressure control mechanism (not shown in the figure).
  • the air circuit control assembly is respectively connected with the second joint 522, the fourth joint 532, and the pressure control mechanism.
  • the air circuit control assembly has a first working state and a second working state. When the air circuit control assembly works in the first working state, it is used to control the second joint 522 to communicate with the pressure control mechanism, and the fourth joint 532 communicates with the atmospheric environment; when the air circuit control assembly works in the second working state, it is used for The control second joint 522 communicates with the atmospheric environment, and the fourth joint 532 communicates with the pressure control mechanism.
  • the reagent cartridge 40 is loaded into the first rack 10, and the first driving mechanism 51 moves the first air tube 52 and the second air tube 53 to the first A position, so that the first joint 521 is connected to the first air hole 41, and the third joint 531 is connected to the second air hole 42;
  • the air circuit control assembly is in the first working state, the first air hole 41 passes through the first air pipe 52 Connected with the pressure control mechanism, the second air hole 42 communicates with the atmosphere through the second air pipe 53, the suction force provided by the pressure control mechanism acts on the reagent cartridge 40, so that the sample liquid inside the reagent cartridge 40 is between the chambers Transfer;
  • the second air hole 42 is connected to the pressure control mechanism through the second air pipe 53, and the first air hole 41 communicates with the atmospheric environment through the first air pipe 52, and the pressure control
  • the suction force provided by the mechanism acts on the reagent cartridge 40, so that
  • the first air hole 41 of the reagent cartridge 40 can be communicated with the pressure control mechanism through the first air pipe 52, and the second air hole 42 of the reagent cartridge 40 can also be realized through the second air pipe 53. It communicates with the pressure control mechanism, and can provide power according to the technological requirements of the reagent cartridge 40 to promote the transfer of the sample liquid between the chambers, and at the same time, the overall structure of the PCR module is relatively simplified.
  • both the first joint 521 and the third joint 531 are vacuum suction cups.
  • the vacuum chuck completely covers the outside of the wall of the first air hole 41, which can ensure the butt tightness between the first joint 521 and the first air hole 41; similarly, when the third joint 531 connects with the second air hole 42 , the vacuum chuck can completely cover the outside of the wall of the second air hole 42 , which can ensure the butt tightness between the third joint 531 and the second air hole 42 .
  • the air circuit control components include, for example, electromagnetic control valves and pipelines.
  • the electromagnetic control valve communicates with the second joint 522, the fourth joint 532 and the pressure control mechanism respectively through pipelines.
  • the pressure control mechanism is specifically a vacuum pump, an exhaust fan, a plunger pump and the like.
  • the electromagnetic control valve is provided with a first working state and a second working state. When the electromagnetic control valve switches the working state, the first air hole 41 of the reagent cartridge 40 can be communicated with the pressure control mechanism through the first air pipe 52, and realize the pressure control mechanism of the reagent cartridge.
  • the second air hole 42 of 40 communicates with the pressure control mechanism through the second air pipe 53 .
  • the number of electromagnetic control valves installed, the specific structure of the pipeline, and the installation position of the electromagnetic control valve on the pipeline are not limited here, and are set according to actual needs, as long as at least the above-mentioned first working state can be achieved And the second working state can be.
  • the second joint 522 can be connected to the air pump through the pipeline, or the second joint 522 can be communicated with the atmospheric environment through the pipeline, and the other electromagnetic control valve
  • the synchronous action can realize that the fourth joint 532 is connected with the atmospheric environment through the pipeline, or the fourth joint 532 is connected with the air pump through the pipeline.
  • the PCR module further includes a second pressing component 60 .
  • the second pressing assembly 60 includes a second driving mechanism 61 and a second mounting plate 63 .
  • the second driving mechanism 61 is arranged on the first bracket 10, and the second driving mechanism 61 is connected with the second mounting plate 63, and the second driving mechanism 61 is used to drive the second mounting plate 63 to move close to or away from the direction of the reagent cartridge 40 .
  • the window assembly 30 is disposed on the second mounting board 63 . In this way, the second driving mechanism 61 drives the second mounting plate 63 to move toward or away from the reagent cartridge 40.
  • the second mounting plate 63 When the second mounting plate 63 moves, it drives the window assembly 30 to move, so that the transparent plate 311 of the window assembly 30 can be tightly contacted. on the other side of the PCR chamber 49, or release the other side of the PCR chamber 49. That is, when the reagent cartridge 40 is disassembled on the first bracket 10, the transparent plate 311 is kept away from the other side of the PCR chamber 49 under the driving action of the second drive mechanism 61, so as to avoid damage to the reagent cartridge 40. cause interference; when the reagent cartridge 40 is put in place on the first rack 10 , the transparent plate 311 is tightly contacted against the other side of the PCR chamber 49 under the driving action of the second driving mechanism 61 .
  • the first drive mechanism 51 is, for example, a motor screw drive structure, a cylinder drive structure, a hydraulic cylinder drive structure, an electric cylinder drive structure, a cam drive structure, etc., which are not limited here, and can be set according to actual needs. Can.
  • the second driving mechanism 61 is similar to the first driving mechanism 51 , and details are not repeated here.
  • the window assembly 30 further includes a first elastic member 32 .
  • the optical window 31 is connected to the second mounting plate 63 through the first elastic member 32 .
  • the first elastic member 32 acts as a buffer to ensure that the transparent plate 311 is in close contact with the side of the PCR chamber 49 while avoiding damage to the PCR chamber 49.
  • the window assembly 30 further includes a first carrier plate 33 disposed between the first elastic member 32 and the optical window 31 .
  • the optical window 31 is disposed on the first carrier board 33
  • the first carrier board 33 is connected to the second mounting board 63 through the first elastic member 32 .
  • the first carrier plate 33 is made of a hard material such as aluminum alloy, which can strengthen the structural strength of the optical window 31 and is not easy to be damaged.
  • the number of the first elastic member 32 is not limited here, for example, it can be one, two, three or other numbers, which can be set according to actual needs.
  • the second mounting plate 63, the first carrier plate 33, and the PC board 312 of the optical window 31 are provided with a transparent plate 311.
  • the corresponding hollow opening 34 can allow the fluorescence of the optical detection module 100 to pass through.
  • the second mounting plate 63 is provided with a first concave portion 631 , and the first elastic member 32 and the first carrier plate 33 are both disposed in the first concave portion 631 .
  • a first limiting plate 64 is arranged on the surface of the second mounting plate 63, and the first limiting plate 64 is in conflict with the first carrier plate 33, and the first limiting plate 64 is circumferentially arranged around the outer edge of the optical window 31 .
  • the first carrier plate 33 plays a role of limiting the first carrier 33 , and can prevent the first carrier 33 and the first elastic member 32 from coming out of the first recess 631 .
  • the second pressing assembly 60 further includes at least one second pressing member 62 .
  • At least one second pressing member 62 is installed on the second mounting plate 63 , and the second pressing member 62 is used for pressing or releasing the second valve 45 of the reagent cartridge 40 .
  • the number of the first pressing members 56 installed on the first mounting plate 54 is not less than the number of the first valves 44 of the reagent cartridge 40, and the second mounting plate The quantity of the second pressing members 62 installed on the 63 is not less than the quantity of the second valves 45 of the reagent cartridge 40 .
  • the first driving mechanism 51 drives the first mounting plate 54 to move, all the first valves 44 of the reagent cartridge 40 can be opened or closed; similarly, the second driving mechanism 61 drives the second mounting plate 63 to move , all the second valves 45 of the reagent cartridge 40 can be opened or closed.
  • the number of the first pressing parts 56 in this embodiment is four, among which the three first pressing parts 56 are provided in one-to-one correspondence with the three first valves 44 of the reagent cartridge 40, and the other third A pressing member 56 is also used to compress or loosen the reagent cartridge 40 , but not at the position where the first valve 44 is provided, so as to ensure a better pressing effect on the side of the reagent cartridge 40 .
  • the number of the second pressing members 62 in this embodiment is, for example, four, and the four second pressing members 62 are provided in one-to-one correspondence with the four second valves 45 of the reagent cartridge 40 .
  • the first pressing member 56 of the first pressing assembly 50 and the second pressing member 62 of the second pressing assembly 60 are collectively arranged on the same mounting plate, that is, there is no need to set two As for the mounting plate, a driving mechanism is used to drive the mounting plate on which the first pressing member 56 and the second pressing member 62 are installed to move.
  • a third pressing assembly or a fourth pressing assembly is also provided, which is not limited here.
  • the first pressing assembly 50 further includes at least one second elastic member 57 mounted on the first mounting plate 54 .
  • At least one first pressing member 56 is provided in one-to-one correspondence with at least one second elastic member 57 , and the first pressing member 56 is connected to the first mounting plate 54 through the second elastic member 57 . In this way, under the buffering effect of the second elastic member 57 , the reagent cartridge 40 can be avoided from being damaged when the first pressing member 56 contacts the first valve 44 .
  • the second pressing assembly 60 also includes at least one third elastic member (not shown in the figure) installed on the second mounting plate 63, at least one second pressing member 62 and at least one third elastic member One corresponding setting, the second pressing member 62 is connected with the second mounting plate 63 through the third elastic member.
  • the first mounting plate 54 is provided with a first accommodation chamber 541 and a first guide through hole 542 communicating with the first accommodation chamber 541 , and the second elastic member 57 is disposed in the first accommodation chamber 541 .
  • the first pressing member 56 is located inside the first accommodating chamber 541 and connected with the second elastic member 57.
  • the first pressing member 56 is movably arranged in the first guide through hole 542, and the first pressing member 56 The other end of the tight member 56 extends out of the first guide through hole 542 and is used for pressing or releasing the first valve 44 of the reagent cartridge 40 .
  • the second mounting plate 63 is provided with a second accommodation chamber and a second guide through hole (not shown in the figure) communicating with the second accommodation chamber, the third elastic member is arranged in the second accommodation chamber, and the second pressing One end of the pressing member 62 is located inside the second accommodation chamber and is connected with the third elastic member.
  • the second pressing member 62 is movably arranged in the second guide through hole, and the other end of the second pressing member 62 protrudes to the first
  • the second valve 45 guides the outside of the through hole and is used to compress or loosen the reagent cartridge 40 .
  • the PCR module further includes a slide assembly 70 .
  • the slide rail assembly 70 includes a fixed frame 71 installed on the first bracket 10, a first slide rail 721011 and a second slide rail 731011 arranged on the fixed frame 71, and a first slide rail 721011 slidably arranged on the first slide rail 721011.
  • the first connecting plate 76 is connected to the first mounting plate 54
  • the second connecting plate 77 is connected to the second mounting plate 63 .
  • the movement stroke of the first mounting plate 54 can be controlled more precisely, that is, the movement stroke accuracy of the first pressing member 56 can reach 0.05mm.
  • the first pressing member 56 since the movement stroke accuracy of the first pressing member 56 is relatively high, when the first valve 44 is opened, under the power of the first driving mechanism 51, the first pressing member 56 is far away from the first valve 44. Loosen the first valve 44 of the reagent cartridge 40 by a moving distance of, for example, 0.1 mm or 0.2 mm. At this time, the first pressing member 56 is still in contact with the side of the reagent cartridge 40 to play a positioning role, so that the reagent cartridge 40 is stably set on the first bracket 10.
  • the second driving mechanism 61 drives the second mounting plate 63 to move
  • the second connecting plate 77 drives the second slider 75 to move along the second slide rail 731011, so that the running stability of the second mounting plate 63 is better, and then it can
  • the movement stroke of the second mounting plate 63 is controlled more precisely, that is, the movement stroke accuracy of the second pressing member 62 reaches 0.05 mm. Because the motion stroke accuracy of the second pressing member 62 is relatively high, when the second valve 45 is opened, under the power of the second drive mechanism 61, the second pressing member 62 moves away from the second valve 45. For example, loosen the second valve 45 of the reagent cartridge 40 by 0.1mm or 0.2mm.
  • the second pressing member 62 is still in contact with the side of the reagent cartridge 40 to play a positioning role, so that the reagent cartridge 40 is stably arranged on the second valve.
  • the first joint 521 and the third joint 531 are respectively connected to the first air hole 41 and the second air hole 42 of the reagent cartridge 40, and the transparent plate 311 and the other side of the PCR chamber 49 are still connected to each other. in a state of conflict.
  • first mounting plate 54, the second mounting plate 63, the first slider 74, the second slider 75, the first connecting plate 76 and the second connecting plate 77 are specifically made of aluminum, copper, iron, etc. , stainless steel, wood, plastic, etc., are not limited here, and can be set according to actual needs.
  • the PCR module further includes a first sensor 104181 and a first trigger 104282 that inductively cooperates with the first sensor 104181 .
  • the first sensor 104181 is mounted on the first support 10
  • the first trigger 104282 is mounted on the first mounting plate 54 or the first connecting plate 76 .
  • the first sensor 104181 can sense the moving position of the first triggering part 104282, and the moving position information of the first mounting plate 54 can be obtained according to the sensing signal of the first triggering part 104282, and then the first pressing part can be controlled accordingly 56's motion stroke is within the preset range.
  • the PCR module further includes a second sensor 83 and a second trigger 84 that inductively cooperates with the second sensor 83 .
  • the second sensor 83 is mounted on the support board 11
  • the second trigger 84 is mounted on the second installation board 63 or the second connection board 77 .
  • the first sensor 104181 is a photoelectric switch, a proximity switch, a through-beam sensor or other types of sensors, as long as it can sense the moving position of the first trigger 104282 and feedback the moving position signal of the first trigger 104282 in time That's it.
  • the second sensor 83 is similar to the first sensor 104181, and will not be repeated here.
  • the second pressing assembly 60 further includes at least one third elastic member installed on the second mounting plate 63, and at least one second pressing member 62 is provided in a one-to-one correspondence with the at least one third elastic member. .
  • the second pressing member 62 is connected with the second mounting plate 63 through the third elastic member. Likewise, under the buffering effect of the third elastic member, the second pressing member 62 can avoid damage to the reagent cartridge 40 during the process of contacting the second valve 45 .
  • a first receiving chamber 541 and a first guiding through hole 542 communicating with the first receiving chamber 541 are disposed inside the first mounting plate 54 .
  • the second elastic member 57 is arranged in the first accommodation chamber 541, and one end of the first pressing member 56 is located inside the first accommodation chamber 541 and is connected with the second elastic member 57.
  • the first pressing member 56 is movably arranged in the second In a guiding through hole 542 , the other end of the first pressing member 56 extends out of the first guiding through hole 542 and is used for pressing or releasing the first valve 44 of the reagent cartridge 40 .
  • the second mounting plate 63 is provided with a second accommodation chamber (not shown in the figure) and a second guide through hole (not shown in the figure) communicating with the second accommodation chamber.
  • the third elastic member is disposed in the second accommodation chamber.
  • One end of the second pressing member 62 is located inside the second accommodation chamber and is connected with the third elastic member.
  • the second pressing member 62 is movably arranged in the second guide through hole, and the other end of the second pressing member 62 extends It goes out to the outside of the second guide through hole and is used to compress or loosen the second valve 45 of the reagent cartridge 40 .
  • the first pressing assembly 50 further includes a fifth elastic member 55 .
  • the first air pipe 52 is connected to the first mounting plate 54 through the fifth elastic member 55 .
  • the fifth elastic member 55 acts as a buffer to prevent the first joint 521 of the first air tube 52 from connecting with the first air hole 41 of the reagent cartridge 40. 521 causes damage to the reagent cartridge 40.
  • the first mounting plate 54 is provided with a third guide through hole 543
  • the first air pipe 52 is movably disposed in the third guide through hole 543
  • the wall of the third guide through hole 543 is provided with a first A step 544
  • a second step 523 is provided on the outer wall of the first air pipe 52
  • the two ends of the fifth elastic member 55 are in contact with the first step 544 and the second step 523 respectively.
  • the fifth elastic member 55 is a spring sheathed on the outer wall of the trachea, and the two ends of the spring are in conflict with the first step 544 and the second step 523 respectively.
  • the first elastic member 32, the second elastic member 57, the third elastic member, the fourth elastic member 203, and the fifth elastic member 55 are, for example, springs, elastic blocks, etc., which are not limited here. You can set it as needed.
  • the installation method of the second air pipe 53 on the first mounting plate 54 is similar to that of the first air pipe 52 , which will not be repeated here.
  • the first bracket 10 includes a support plate 11 and a guide plate 12 .
  • One side of the support plate 11 is provided with a second concave portion 111 , and the second concave portion 111 is adapted to the reagent cartridge 40 .
  • the guide plate 12 is disposed on one side of the support plate 11 , and the guide plate 12 is used to resist the side of the reagent cartridge 40 loaded into the second concave portion 111 .
  • One sidewall of the second concave portion 111 is provided with an inlet and outlet 112 .
  • the reagent cartridge 40 enters the second recess 111 along the guide plate 12 through the inlet and outlet 112, that is, the guide plate 12 guides the reagent cartridge 40, and the reagent cartridge After the box 40 enters the second recess 111 , the position is relatively stable under the action of the guide plate 12 and the support plate 11 .
  • the PCR module further includes a pyrolysis component 91 .
  • the thermal cracking assembly 91 is installed on the support plate 11 and is used for thermally cracking the liquid in the pretreatment chamber 47 of the reagent cartridge 40 .
  • the PCR module also includes a magnetic mixing assembly 92.
  • the magnetic mixing assembly 92 is installed on the support plate 11, and is used to move the magnetic beads in the mixing chamber 48 of the reagent cartridge 40 up and down, so that the magnetic beads in the mixing chamber 48 The liquid is thoroughly mixed and processed to obtain the desired mixed liquid.
  • At least one first via hole 121 (not shown in the figure) and at least one second via hole 122 (not shown in the figure) are opened. Let the first pressing piece 56 pass through. At least one second via hole 122 is provided corresponding to the first joint 521 of the first air pipe 52, and is also provided corresponding to the third joint 531 of the second air pipe 53, allowing the first joint 521 to pass through and the third joint to pass through. 531 across.
  • the PCR module further includes a first sensor 104181 and a first trigger 104282 that inductively cooperates with the first sensor 104181 .
  • the first sensor 104181 is mounted on the first support 10
  • the first trigger 104282 is mounted on the first mounting plate 54 or the first connecting plate 76 .
  • the first sensor 104181 can sense the moving position of the first triggering part 104282, and the moving position information of the first mounting plate 54 can be obtained according to the sensing signal of the first triggering part 104282, and then the first pressing part can be controlled accordingly 56's motion stroke is within the preset range.
  • the PCR module further includes a second sensor 83 and a second trigger 84 that inductively cooperates with the second sensor 83 .
  • the second sensor 83 is mounted on the support board 11
  • the second trigger 84 is mounted on the second installation board 63 or the second connection board 77 .
  • the first sensor 104181 is a photoelectric switch, a proximity switch, a through-beam sensor or other types of sensors, as long as it can sense the moving position of the first trigger 104282 and feedback the moving position signal of the first trigger 104282 in time That's it.
  • the second sensor 83 is similar to the first sensor 104181, and will not be repeated here.
  • an in vitro diagnostic testing device includes the PCR module of any one of the above-mentioned embodiments.
  • the reagent cartridge 40 is installed on the first bracket 10, so that the heat conduction plate 23 is in close contact with one side of the PCR chamber 49, and the transparent plate 311 is in contact with the side of the PCR chamber 49. The other side is in close contact with each other, and then the semiconductor refrigerator 22 performs cyclic heating and cooling actions.
  • heating the heat is transferred to the PCR chamber 49 through the heat conduction plate 23, and when the temperature is lowered, the heat is transferred from the PCR chamber 49 to the substrate through the heat conduction plate 23.
  • the heat is transferred to the external environment by the heat sink, so that the PCR chamber 49 is cooled, and at the same time, the excitation light from the optical detection module 100 is injected into the PCR chamber 49 through the transparent plate 311 of the optical window 31
  • the sample liquid generates fluorescence and reflects back to the optical detection module 100 for optical detection, which can perform quantitative detection of the sample amplification quantity in each thermal cycle.
  • the PCR chamber 49 of the reagent cartridge 40 has a certain pressure and slightly bulges, after being compressed by the optical window 31, the other side of the PCR chamber 49 has a good contact with the heat conducting plate 23, thereby greatly reducing the material interface.
  • the overall structure of the PCR module is relatively simple.
  • the PCR module further includes a temperature sensor (not shown in the figure).
  • the temperature sensor is used to obtain temperature information of the heat conducting plate 23 .
  • the temperature information of the heat conduction plate 23 is sensed in real time by the temperature sensor, so that the temperature of the heat conduction plate 23 can be accurately grasped, and the semiconductor refrigerator 22 can be controlled to work according to the detected temperature information.
  • the semiconductor refrigerator 22 compares the temperature values returned by the two temperature sensors synchronously. If the two detected temperature values are out of tolerance, an error will be reported, which can ensure that the temperature sensor is not invalid.
  • the temperature of the heat conduction plate 23 is controlled by a PID algorithm to ensure the accuracy and reliability of the temperature of the heat conduction plate 23 .
  • Fig. 18 shows another perspective structural view of the first bracket 10 according to an embodiment of the present disclosure
  • Fig. 19 shows the guide plate in the structure shown in Fig. 18 12 Schematic diagram of the enlarged structure after hiding and at m.
  • the PCR module further includes a limiting baffle 16 and a sixth elastic member 17 .
  • the guide plate 12 is disposed on one side of the support plate 11 , and the guide plate 12 is used to resist the side of the reagent cartridge 40 loaded into the second concave portion 111 .
  • One side wall of the second recess 111 is provided with an inlet and outlet 112 , and the other side wall of the second recess 111 is provided with an activity chamber 115 .
  • the activity chamber 115 communicates with the second recess 111 .
  • the limiting baffle 16 is movably disposed in the movable chamber 115 , the limiting baffle 16 is connected to the wall of the movable chamber 115 through the sixth elastic member 17 , and the limiting baffle 16 is provided with a blocking portion 161 .
  • the blocking portion 161 is used to interfere with the reagent cartridge 40 loaded into the second concave portion 111 .
  • the reagent cartridge 40 enters the second recess 111 along the guide plate 12 through the inlet and outlet 112, that is, the guide plate 12 guides the reagent cartridge 40, and the reagent cartridge After the cartridge 40 enters the second recess 111, the position is relatively stable under the action of the guide plate 12 and the support plate 11, which can ensure that the relative position of the reagent cartridge 40 is relatively accurate, and facilitates the alignment of the reagent cartridge 40 with the first pressing member 56 , the second pressing member 62, the first joint 521, the second joint 522, the third joint 531, and the fourth joint 532; in addition, under the elastic force of the sixth elastic member 17, the limit baffle 16
  • the blocking part 161 is in contact with the reagent cartridge 40 with a certain pressure, so that the reagent cartridge 40 will not be easily disengaged from the inlet and outlet 112, that is, the reagent cartridge 40 can be firmly arranged on the valve control assembly of the cartridge, so that
  • the stopper 16 is driven to move to separate the blocking portion 161 from the reagent cartridge 40 , and the reagent cartridge 40 is taken out through the inlet and outlet 112 .
  • one end of the limit baffle 16 is rotatably connected to the wall of the movable chamber 115 through the rotating member 162 .
  • the other end of the limiting baffle 16 is connected to the wall of the movable chamber 115 through the sixth elastic member 17 , and the other end of the limiting baffle 16 is also provided with a blocking portion 161 .
  • the blocking portion 161 is provided with a guide edge 1611 , and the guide edge 1611 is inclined toward the inlet and outlet 112 .
  • the inclination angle of the guide edge 1611 relative to the direction in which the reagent cartridge 40 enters and exits the second recess 111 is a, and a is 30° to 60°.
  • a can be any angle from 0° to 90°, which is not limited here. , and set it according to actual needs.
  • the protruding part of the reagent cartridge 40 touches the guide edge 1611, and gradually enters the second recess 111 under the guidance of the guide edge 1611, and at the same time
  • the sixth elastic member 17 is pushed and compressed by the guide edge 1611; when the reagent cartridge 40 is moved in place, the blocking part 161 is reset and moved under the action of the sixth elastic member 17 and then interferes with the protrusion of the reagent cartridge 40 in a limited position, thereby It can play the role of snapping against the protruding part of the reagent cartridge 40 to prevent the reagent cartridge 40 from coming out through the inlet and outlet 112 .
  • the working method of the cartridge valve control assembly includes the following steps:
  • Step S10 adding samples. Put the patient sample into the sample chamber 46 of the reagent cartridge 40 , close the cover of the reagent cartridge 40 and insert the reagent cartridge 40 into the first bracket 10 .
  • the limit baffle 16 acts as a limit and can prevent the reagent cartridge 40 from falling off from the first bracket 10 .
  • Step S20 after installing the reagent cartridge 40 on the first bracket 10, the first pressing assembly 50 and the second pressing assembly 60 work, the first driving mechanism 51 drives the first mounting plate 54 to move close to the first bracket 10, the second The second drive mechanism 61 drives the second mounting plate 63 to move close to the first bracket 10, so that the first joint 521 is connected to the first air hole 41, and the third joint 531 is connected to the second air hole 42.
  • the three first pressing members 56 are in one-to-one correspondence with the three first valves 44 of the reagent cartridge 40, the other first pressing member 56 is in contact with the reagent cartridge 40, and the four second pressing members 62 are in contact with the reagent card in one-to-one correspondence.
  • the four second valves 45 of the cartridge 40 make the reagent cartridge 40 clamped and fixed.
  • Step S30 after the reagent cartridge 40 is completely clamped, control the air circuit control assembly to work in the first working state, so that the second air hole 42 communicates with the atmosphere, the first air hole 41 communicates with the plunger pump, and the plunger pump passes through
  • the reverse movement of the piston provides a negative pressure to the first air hole 41 , so that the sample liquid in the reagent cartridge 40 moves from the sample chamber to the pretreatment chamber 47 .
  • Step S40 after the sample liquid is transferred to fill the pretreatment chamber 47, the first driving mechanism 51 is controlled to move the first mounting plate 54 so that the first mounting plate 54 drives the first pressing member 56 away from the reagent cartridge 40 by a preset distance, It only needs to make the three first valves 44 of the reagent cartridge 40 open.
  • the air circuit control assembly is controlled to work in the second working state, so that the first air hole 41 communicates with the atmospheric environment, and the second air hole 42 communicates with the plunger pump, and the plunger pump provides a second air hole 42 through the reverse movement of the piston. Negative pressure. At this time, the lysed sample liquid will be sucked into two mixing chambers 48 to redissolve the freeze-dried reagent therein.
  • Step S50 driving the first driving mechanism 51 to move a preset distance, so that the three first pressing members 56 are in close contact with the three first valves 44 of the reagent cartridge 40 in one-to-one correspondence, that is, the three first valves 44 are closed, And turn on the magnetic mixing assembly 92, by periodically turning on and off the power of the magnetic mixing assembly 92, it will generate periodic magnetic force, drive the magnetic beads arranged in the mixing chamber 48 to reciprocate up and down, and reconstitute the freeze-dried reagent and sample The liquid is mixed well.
  • Step S60 after the mixing is completed, the second drive mechanism 61 is first driven to move a preset distance, so that the four second pressing members 62 are separated from the four second valves 45 , so that the four second valves 45 are opened.
  • the second air hole 42 is pressurized by the positive movement of the piston in the plunger pump, allowing the sample to flow from the mixing chamber 48 into the two PCR chambers 49 .
  • Step S70 under the condition of maintaining a certain pressure, close the four second valves 45 through the four second pressing members 62 tightly contacting the four second valves 45 , so that the PCR cavity of the reagent cartridge 40 is slightly inflated.
  • the optical window 31 corresponding to the PCR chamber 49 can also press against the front of the PCR chamber 49 of the reagent cartridge 40 . Because the PCR chamber 49 of the reagent cartridge 40 has a certain pressure, it bulges slightly, so that it is closely attached to the PCR optical window 31, and the next operation is performed.
  • Step S80 the first driving mechanism 51 and the second driving mechanism 61 reset, the first pressing member 56, the second pressing member 62, the first joint 521 and the third joint 531 all loosen the reagent cartridge 40, and the plunger The pump resets.
  • FIG. 1 shows a schematic structural view of a temperature regulating member 201 according to an embodiment of the present disclosure
  • FIG. 23 shows a design of the temperature regulating member 201 according to an embodiment of the present disclosure.
  • FIG. 24 shows a schematic cross-sectional structural schematic diagram at p-p of FIG.
  • a temperature control component for temperature regulation includes: a temperature adjustment part 201 and a heat conduction block 202, the temperature adjustment part 201 is connected to the heat conduction block 202, the temperature adjustment part 201 is used to generate heat or cold and transmit it to the heat conduction block 202, and the heat conduction
  • the block 202 is provided with a first heat conducting surface 2021 for interference fit with the side of the chamber of the reagent cartridge 40 .
  • the chamber can be the pretreatment chamber 47 for the sample liquid, or the PCR chamber 49 for the sample liquid, etc., which is not limited here.
  • the first heat-conducting surface 2021 is tightly matched with the side of the chamber of the reagent cartridge 40, and the temperature regulating member 201 works to regulate the temperature.
  • the element 201 transfers heat or cold to the heat conduction block 202 , and the heat or cold is transferred to the side of the chamber through the first heat conduction surface 2021 of the heat conduction block 202 .
  • the first heat transfer surface 2021 and the side of the chamber are used to transfer energy in a manner that interferes with the side of the chamber, which can make the energy of the first heat transfer surface 2021 It is better transmitted to the sample liquid in the chamber, and the sample liquid is heated relatively uniformly, which can achieve the expected heating effect or cooling effect, and can ensure accurate and reliable detection results.
  • the reagent cartridge 40 in this embodiment specifically includes a plate and two films respectively located on two opposite sides of the plate, the plate is provided with a window, and the two films and the window are enclosed to form a A chamber for the sample liquid is provided.
  • the size of the opening of the window is much larger than the distance between the two opposite sides of the plate.
  • the ratio of the aperture size of the window to the distance between the two opposite sides is, for example, 5:1, 10:1, 20:1 or other In this way, when the first heat conduction surface 2021 interferes with the side of the chamber to transfer energy, the energy of the first heat conduction surface 2021 can be better transferred to the sample liquid in the chamber, and the sample liquid is heated more uniformly , can achieve the expected heating effect or cooling effect, and can ensure accurate and reliable detection results.
  • heat conduction block 202 there is one heat conduction block 202 and it is set against one side of the chamber of the reagent cartridge 40; in addition, there may be two heat conduction blocks 202, and they are set against one side of the reagent cartridge 40 correspondingly. Both sides of the chamber, that is, both sides of the chamber of the reagent cartridge 40 are heated or refrigerated synchronously.
  • the heat conduction block 202 is specifically, for example, a metal block, and the metal block may be an aluminum block, a copper block, or the like.
  • the heat conduction block 202 is not limited to be a metal block, and other non-metallic materials with better heat conduction performance can also be selected, and the arrangement is made according to actual needs.
  • the heat conduction block 202 serves as an intermediate component between the temperature adjustment member 201 and the chamber of the reagent cartridge 40 , and can transfer the heat or cold generated by the temperature adjustment member 201 to the side of the chamber more uniformly.
  • the shape of the first heat conducting surface 2021 is adapted to the shape of the side of the chamber of the reagent cartridge 40 .
  • the shape of the first heat conducting surface 2021 is correspondingly circular, oval, square, or hexagonal.
  • the first heat conducting surface 2021 can cover the side of the chamber of the reagent cartridge 40 .
  • the first heat-conducting surface 2021 when the first heat-conducting surface 2021 is in conflict with the side of the chamber, it can be ensured that the sides of the chamber are in contact with the first heat-conducting surface 2021, so that the first heat-conducting surface 2021 can evenly transfer heat to the side of the chamber , so that the sample liquid is heated more uniformly, can achieve the expected heating effect or cooling effect, and can ensure accurate and reliable detection results.
  • FIG. 21 shows a schematic cross-sectional view of n-n in FIG. 20
  • FIG. 22 shows a schematic structural view of another viewing angle of the temperature regulating member 201 according to an embodiment of the present disclosure.
  • the PCR module further includes a fourth elastic member 203 and a fixing base 204 .
  • the heat conduction block 202 also has a second heat conduction surface 2022 opposite to the first heat conduction surface 2021 .
  • One side of the temperature regulating member 201 is in contact with the second heat conducting surface 2022 , and the other side of the temperature regulating member 201 is connected to the fixing seat 204 through the fourth elastic member 203 .
  • the heat conduction block 202 can be tightly contacted against the side of the chamber with a certain pressure, so that heat or cold can be better transferred to the sample liquid in the chamber, and the sample The liquid is heated evenly, which can achieve the expected heating effect or cooling effect, and can ensure the accuracy and reliability of the detection results; The force should not be too great to cause damage to the film.
  • the fourth elastic member 203 may be, for example, a spring, an elastic block, etc., which is not limited here, and can be set according to actual needs.
  • one end of the fourth elastic member 203 is connected to or interferes with the temperature regulating member 201
  • the other end of the fourth elastic member 203 is connected to or interferes with the fixing seat 204 .
  • One end of the fourth elastic member 203 is in conflict with the temperature regulating member 201, specifically the middle part of the side of the temperature regulating member 201; in addition, when the other end of the fourth elastic member 203 is in conflict with the fixing seat 204,
  • An insertion hole 2041 (as shown in FIG. 2 ) is disposed on the fixing seat 204 , and the other end of the fourth elastic member 203 extends into the insertion hole 2041 and collides with the wall of the insertion hole 2041 .
  • the support plate 11 is provided with a first installation hole 114 extending from one side of the support plate 11 to the other side.
  • the heat conduction block 202 is movably disposed in the first installation hole 114 .
  • the fixing seat 204 is arranged on one side of the support plate 11 (the right side as shown in FIG. 24 ), and the first heat conducting surface 2021 protrudes to the other side of the support plate 11 (the left side as shown in FIG. ) outside. In this way, the reagent cartridge 40 is installed on the other side of the support plate 11.
  • the first heat conducting surface 2021 protrudes to the support plate 11
  • the outside of the other side of the reagent cartridge 40 that is, the first heat conducting surface 2021 protrudes to the outside of the first mounting hole 114 , so that the first heat conducting surface 2021 can facilitate a tight interference fit with the side of the chamber of the reagent cartridge 40 .
  • the height of the first heat conduction surface 2021 protruding to the outside of the other side of the support plate 11 is, for example, 0.5 mm, 1 mm, 2 mm or 5 mm. etc.
  • the heat conduction block 202 includes a heat conduction portion 2023 and a first flange 2024 circumferentially disposed around the heat conduction portion 2023 .
  • the first flange 2024 interferes with one side of the support plate 11 , the heat conducting part 2023 is movably disposed in the first installation hole 114 , and the first heat conducting surface 2021 is disposed on the heat conducting part 2023 .
  • the fourth elastic member 203 makes the first flange 2024 tightly contact with one side of the support plate 11 , the first flange 2024 serves to limit the position of the heat conduction block 202 , and at this time the first heat conduction surface 2021 of the heat conduction portion 2023 protrudes to the outside of the other side of the support plate 11 to a maximum height.
  • the heat conduction part 2023 is arranged in accordance with the first installation hole 114 , so that the heat conduction part 2023 has better stability when moving along the first installation hole 114 .
  • the heat conducting part 2023 is, for example, cylindrical, square cylindrical, etc., and the first installation hole 114 is correspondingly circular through hole, square through hole, etc. FIG.
  • the first flange 2024 may not be provided, for example, when the fourth elastic member 203 is connected to the heat conduction block 202 , the position of the heat conduction block 202 is limited by the fourth elastic member 203 .
  • first flange 2024 can be "a part of the heat conduction part 2023", that is, the "first flange 2024” is integrally formed with “other parts of the heat conduction part 2023”;
  • the other parts of 2023" can be separated as an independent component, that is, the "first flange 2024” can be manufactured independently, and then combined with the "other parts of the heat conducting part 2023” to form a whole.
  • the "first flange 2024" is a part of the integrally formed part of the "heat conducting part 2023".
  • a third recess 113 corresponding to the first flange 2024 is provided on one side of the support plate 11 .
  • the first flange 2024 is disposed in the third recess 113 and engages with the bottom wall of the third recess 113 .
  • the first installation hole 114 extends from the bottom wall of the third recess 113 to the other side of the support plate 11 . In this way, after the first flange 2024 of the heat conduction block 202 is disposed in the third recess 113 , the third recess 113 acts as a limit to the first flange 2024 , so that the heat conduction block 202 operates more stably when it is moved under force.
  • the heat conduction portion 2023 can be quickly installed in the first installation hole 114 , and the installation operation of the heat conduction block 202 on the support plate 11 is more convenient.
  • a second recess 111 for accommodating the reagent cartridge 40 is provided on the other side of the support plate 11 .
  • the first installation hole 114 extends from one side of the support plate 11 to the bottom wall of the second recess 111 .
  • the first bracket 10 further includes a guide plate 12 installed on the other side of the support plate 11 , and the guide plate 12 is used to interfere with the side of the reagent cartridge 40 facing away from the heat conducting block 202 .
  • the reagent cartridge 40 is installed in the second recess 111, since the guide plate 12 and the side of the reagent cartridge 40 facing away from the heat conduction block 202 are fixed in contact with each other, the other side of the reagent cartridge 40 is in contact with the second recess 111. conflicts with the bottom wall of the bottom wall, that is, the reagent cartridge 40 can be stably fixed in the second recess 111; in addition, on the basis that the reagent cartridge 40 is firmly fixed in the second recess 111, the first heat conduction surface of the heat conduction block 202 2021 can stably and closely contact the side of the chamber, so that the heat or cold transfer effect is better.
  • the "guide plate 12" can be “a part of the support plate 11", that is, the “guide plate 12” is integrally formed with “other parts of the support plate 11"; An independent component that can be separated, that is, the "guide plate 12" can be manufactured independently, and then combined with the "other parts of the support plate 11" to form a whole.
  • the in vitro diagnostic detection device further includes a temperature sensor 205 .
  • the temperature sensor 205 is used to obtain temperature information of the heat conduction block 202 .
  • the temperature information of the heat conduction block 202 can be sensed in real time by the temperature sensor 205 , so that the temperature of the heat conduction block 202 can be accurately grasped, and the temperature regulating member 201 can be controlled to work according to the detected temperature information.
  • a temperature sensor 205 on the heating element to monitor the temperature of the heating element is limited by the reliability of the performance of the temperature sensor 205 and the reliability of the production and installation process. There will be a certain temperature difference between the actual temperature and the set temperature. Affect the reliability of the test results of the sample liquid.
  • the two temperature sensors 205 are connected to the heat conduction block 202 .
  • the wires 2051 of the two temperature sensors 205 are also used for electrical connection with the controller (not shown in the figure).
  • the temperature adjustment member 201 compares the temperature values returned by the two temperature sensors 205 synchronously. invalidated.
  • a PID algorithm is used to control the temperature of the heat conduction block 202 to ensure accurate and reliable temperature of the heat conduction block 202 .
  • the heat conduction block 202 is provided with a second mounting hole 2025 .
  • the temperature sensor 205 is arranged on the hole wall of the second mounting hole 2025, and the wire 2051 of the temperature sensor 205 protrudes outward through the second mounting hole 2025 and is used for electrically connecting with the controller; the second mounting hole 2025 is filled with thermal paste.
  • the second mounting hole 2025 is, for example, a blind hole, and after the temperature sensor 205 is disposed in the blind hole, it is filled in the blind hole with thermal conductive glue.
  • the temperature sensor 205 is fixed in the blind hole of the heat conduction block 202 , and the heat conduction glue facilitates the transfer of heat to the temperature sensor 205 , which can sense the temperature information of the heat conduction block 202 more sensitively.
  • the second installation hole 2025 can also be a through hole, and after the temperature sensor 205 is installed in the second installation hole 2025 , the second installation hole 2025 is filled with thermal conductive glue.
  • the heat conduction block 202 is provided with a second heat conduction surface 2022 opposite to the first heat conduction surface 2021, and the temperature regulating member 201 is a power resistor, and one side of the power resistor is connected to the first heat conduction surface.
  • the two heat conducting surfaces 2022 are bonded together.
  • the power resistor is fixedly installed on the heat conduction block 202 through the first mounting part 2026 .
  • the first mounting member 2026 is, for example, a screw, a pin, a rivet, a clamping member and the like.
  • the power resistor can also be fixed on the heat conduction block 202 by using heat conduction adhesive.
  • one side of the power resistor is attached to the second heat-conducting surface 2022 through a phase-change heat-conducting sticker. In this way, the heat generated by the power resistor can be better transferred to the heat conducting block 202 .
  • the second heat conduction surface 2022 is provided with several spaced limiting portions 20221 .
  • the several limiting parts 20221 are in conflict with the edge of the power resistor.
  • the several limiting parts 20221 are in conflict with the edge of the power resistor, so that the power resistor and the heat conduction block 202 can be firmly combined together.
  • the heat conduction block 202 is provided with a second heat conduction surface 2022 opposite to the first heat conduction surface 2021 .
  • the temperature regulating member 201 includes a Peltier (not shown in the figure) and a heat dissipation device (not shown in the figure). One side of the Peltier is attached to the second heat conducting surface 2022 , and the other side of the Peltier is attached to the heat dissipation device.
  • the heat conduction block 202 , the Peltier and the heat dissipation device are fixedly connected through a second mounting piece (not shown in the figure). In this way, the Peltier can not only generate heat and transfer the heat to the heat conducting block 202 for heating, but also generate cold energy and transfer the cold energy to the heat conducting block 202 for cooling.
  • the in vitro diagnostic detection device further includes an optical detection module 100 .
  • the optical detection module 100 includes a second bracket 101 , a plurality of optical detection units 102 , and a moving mechanism 103 .
  • a plurality of optical detection units 102 are sequentially arranged on the second bracket 101 at intervals, and the optical detection units 102 are used for fluorescence detection of the sample liquid in the PCR chamber 49 .
  • the moving mechanism 103 is connected with the second bracket 101 , and the moving mechanism 103 is used to drive the second bracket 101 to move, so that the plurality of optical detection units 102 move to positions opposite to the PCR chamber 49 in sequence.
  • the above-mentioned optical detection module 100 when it is necessary to perform fluorescence detection on the PCR chamber 49, drives the second support 101 to move through the moving mechanism 103, so that the plurality of optical detection units 102 are sequentially moved to positions opposite to the PCR chamber 49, Fluorescence detection is performed on the PCR chamber 49 in turn.
  • the method of driving multiple optical detection units 102 to move sequentially along the vertical or horizontal direction to the position opposite to the PCR chamber 49 for fluorescence detection has a faster switching speed , which is more convenient and can improve the efficiency of fluorescence detection; on the other hand, the overall structure of the optical detection module 100 is relatively simple, and the multiple optical detection units 102 are independent of each other, which is convenient for maintenance and has a long service life.
  • a plurality of optical detection units 102 are sequentially arranged on the second support 101 at intervals along the first direction, and the moving mechanism 103 is used to drive the second support 101 along the first direction. direction to move.
  • the first direction is a vertical direction, a horizontal direction, or a direction having an included angle with the vertical direction.
  • the first direction is specifically shown by f in FIG. 28 . It should be noted that the first direction is a direction that forms an included angle with the vertical direction, that is, a direction that is inclined relative to the vertical direction.
  • FIG. 30 and FIG. 31 respectively show two structural diagrams of the moving mechanism 103 according to an embodiment from different viewing angles.
  • the moving mechanism 103 includes a third bracket 1031 , a first motor 1032 , a first driving wheel 1033 , a second driving wheel 1034 , and a transmission element 1035 connecting the first driving wheel 1033 and the second driving wheel 1034 .
  • the first motor 1032 , the first driving wheel 1033 and the second driving wheel 1034 are all arranged on the third bracket 1031 , and the rotating shaft of the first motor 1032 is connected with the first driving wheel 1033 for driving the first driving wheel 1033 to rotate.
  • the transmission element 1035 is also connected with the second bracket 101 .
  • connection between the transmission element 1035 and the second bracket 101 includes but not limited to clamp connection, adhesive connection, or connection with screws, pins, ropes and other mounting parts, so that the transmission element 1035 can drive the second bracket 101 to move when moving.
  • the second bracket 101 is slidably disposed on the third bracket 1031 .
  • the first driving wheel 1033 and the second driving wheel 1034 are specifically, for example, gears, sprockets, pulleys, etc., and the transmission element 1035 is set corresponding to the first driving wheel 1033 and the second driving wheel 1034 .
  • the first drive wheel 1033 drives the transmission element 1035 to move
  • the transmission element 1035 drives the second bracket 101 to run when the transmission element 1035 moves, so as to drive different optical detection units 102 times to move to the PCR chamber 49 relative position.
  • the second bracket 101 is slidably disposed on the third bracket 1031 , that is, the second bracket 101 slides along the third bracket 1031 during operation, the running effect is more stable and reliable.
  • the second bracket 101 is provided with a third slide rail 1011
  • the third bracket 1031 is provided with a third slide block 10311 slidingly matched with the third slide rail 1011 .
  • the moving mechanism 103 is not limited to the above-mentioned combination of the first motor 1032, the first driving wheel 1033, the second driving wheel 1034 and the transmission element 1035, and can also be, for example, a motor screw driving structure, an oil cylinder driving mechanism, The electric cylinder driving mechanism, the air cylinder driving mechanism and the like are not limited here.
  • the optical detection module 100 further includes a first sensor 104181 (as shown in FIG. 25 ) and a first trigger 104282 that inductively cooperates with the first sensor 104181 .
  • the first sensor 104181 is installed on the second bracket 101
  • the first trigger 104282 is installed on the third bracket 1031 .
  • the first sensor 104181 can sense the moving position of the first trigger 104282, and the moving position information of the second bracket 101 can be obtained according to the sensing signal of the first trigger 104282, and then a plurality of optical detection units can be controlled accordingly 102 can be moved to a position opposite to the PCR chamber 49 more accurately.
  • the first sensor 104181 is a photoelectric switch, a proximity switch, a through-beam sensor or other types of sensors, as long as it can sense the moving position of the first trigger 104282 and feedback the moving position signal of the first trigger 104282 in time That's it.
  • each of the plurality of optical detection units 102 includes a light source 1021 .
  • the light sources 1021 of the plurality of optical detection units 102 are light sources 1021 of different wavelength bands.
  • the wavelength band of the fluorescence generated by the optical detection unit 102 is determined according to the wavelength band of the light source 1021, and multiple light sources 1021 of different wavelength bands correspond to a variety of different wavelength bands of fluorescence, that is, they can provide fluorescence of different bands to control the PCR chamber 49. Fluorescence detection is performed on the sample liquid to ensure the effect and efficiency of fluorescence detection, and meet the needs of different detection items at the same time.
  • the number of optical detection units 102 is, for example, five, correspondingly generating fluorescence in five different wavelength bands.
  • the specific band sizes of the five fluorescences are set according to actual needs, and are not limited here.
  • the number of optical detection units 102 may also be other numbers, such as 3, 4, 6, 8, etc., which are not limited here.
  • the plurality of optical detection units 102 there may be two, three or other numbers of optical detection units 102 that provide the same wavelength band of the light source 1021 .
  • the light sources 1021 of the plurality of optical detection units 102 may also be of completely different wavelength bands.
  • the second bracket 101 is provided with a plurality of lighting channels 1012 corresponding to the plurality of optical detection units 102 .
  • the optical detection unit 102 is correspondingly arranged in the lighting channel 1012 .
  • the lighting channel 1012 includes a first channel 10121 and a second channel 10122 .
  • the second channel 10122 is in vertical communication with the middle part of the first channel 10121 , and the light source 1021 is arranged on the end of the second channel 10122 away from the first channel 10121 .
  • the optical detection unit 102 also includes a first lens 1022, a dichroic mirror 1023, a second lens 1024, a third lens 1025, and a fluorescence detector 1026.
  • the first lens 1022 is arranged on the inner wall of the second channel 10122, and the dichroic mirror 1023 is obliquely arranged in the middle part of the first channel 10121, the second lens 1024, the dichroic mirror 1023, the third lens 1025 and the fluorescence detector 1026 are arranged in sequence on the inner wall of the first channel 10121, and the second lens 1024 is located in the first channel 10121.
  • One channel 10121 is close to the end of the PCR chamber 49 , and the fluorescence detector 1026 is located at the end of the first channel 10121 away from the PCR chamber 49 .
  • the light source 1021 is an LED lamp.
  • the fluorescence detector 1026 is specifically a photodiode detector.
  • the optical detection unit 102 further includes a first filter 1027 and a second filter 1028 .
  • the first filter 1027 is disposed on the inner wall of the second channel 10122 , and the first filter 1027 is located between the first lens 1022 and the dichroic mirror 1023 .
  • the second filter 1028 is disposed on the inner wall of the first channel 10121 , and the second filter 1028 is located between the second lens 1024 and the dichroic mirror 1023 .
  • the light source 1021 generates excitation light, and the excitation light is collimated by the first lens 1022 to collimate After the excitation light passes through the first filter 1027, the stray light is filtered out by the first filter 1027, and the excitation light filtered out of the stray light is reflected by the dichroic mirror 1023 and enters the second lens 1024, and the excitation light is converted by the second lens 1024 Converge and irradiate the sample liquid in the PCR chamber of the reagent cartridge 40; the sample liquid at a certain temperature produces fluorescence, which is collected by the second lens 1024 and enters the second filter 1028 through the dichroic mirror 1023, After the second filter 1028 filters the fluorescence, it enters the third lens 1025, and the fluorescence is collected by the third lens 1025 to the fluorescence detector 1026 to detect the
  • step S70 specifically includes the following steps:
  • Step S710 after the sample liquid flows from the mixing chamber 48 to the two PCR chambers 49, under the condition of maintaining a certain pressure, the second driving mechanism 61 drives the mounting plate to move towards the direction close to the reagent cartridge 40, so that the pressing part Pressed against the valve of the reagent cartridge 40, the PCR chamber 49 of the reagent cartridge 40 is in a slightly bulging state.
  • the optical window 31 is also pressed against the front of the PCR chamber 49 synchronously. Because the PCR chamber 49 has a certain pressure to cause it to bulge slightly, after it is compressed by the optical window 31, the back of the PCR chamber 49 has good contact with the heat conducting plate 23, which reduces the heat conduction loss between the material interface, Greatly increased heat transfer efficiency for PCR chamber 49 thermal cycle control.
  • Step S720 the heating and cooling component 20 starts to perform thermal cycle work, for example, the sample liquid in the PCR chamber 49 is first heated to a first preset temperature, and then the sample liquid in the PCR chamber 49 is cooled to a second preset temperature, and Cycle continuously according to the set procedure.
  • the first preset temperature and the second preset temperature are set according to actual conditions, and are not limited here.
  • Step S730 while the heating and cooling assembly 20 heats the sample liquid in the PCR chamber 49, five optical detection units 102 (referred to as the first optical detection unit 102, the second optical detection, and the third optical detection The unit 102, the fourth optical detection unit 102, and the fifth optical detection unit 102) reciprocate up and down through the control of the moving mechanism 103, and stay at 6 positions during the process, and each optical detection unit 102 with a different stop position It will be aligned with the upper and lower PCR cavities of the reagent cartridge 40 in turn. At the same time, when the optical detection unit 102 stays at each position, the light source 1021 on the LED circuit board is turned on sequentially, and the fluorescence detector 1026 collects optical signals.
  • the acquisition timing of the optical module is as follows: the first optical detection unit 102 is aligned with the upper PCR chamber 49 ⁇ the second optical detection unit 102 is aligned with the upper PCR chamber 49, and the first optical detection unit 102 is aligned with the lower PCR chamber 49 ⁇ the third optical detection unit 102 is aligned with the upper PCR chamber 49, the second optical detection unit 102 is aligned with the lower PCR chamber 49 ⁇ the fourth optical detection unit 102 is aligned with the upper PCR chamber 49, the third optical detection unit 102 The detection unit 102 is aligned with the lower PCR chamber 49 ⁇ the fifth optical detection unit 102 is aligned with the upper PCR chamber 49, and the fourth optical detection unit 102 is aligned with the lower PCR chamber 49 ⁇ the fifth optical detection unit 102 Align the lower PCR chamber 49 .
  • This process is a cycle. After one cycle ends, the optical detection module 100 returns to the initial position, that is, the first optical detection unit 102 is aligned with the upper PCR chamber 49, and enters the second cycle. This is repeated, so as to quantitatively detect the number of samples amplified in each thermal cycle cycle.
  • Step S740 after the thermal cycle and optical sampling are completed, the optical detection module 100 returns to the initial position, the second driving mechanism 61 drives the mounting plate to move away from the first bracket 10, and the reagent cartridge 40 is taken out from the first bracket 10 , to complete a nucleic acid amplification and detection.
  • first and second are used for descriptive purposes only, and cannot be interpreted as indicating or implying relative importance or implicitly specifying the quantity of indicated technical features.
  • the features defined as “first” and “second” may explicitly or implicitly include at least one of these features.
  • “plurality” means at least two, such as two, three, etc., unless otherwise specifically defined.
  • a first feature being “on” or “under” a second feature may mean that the first and second features are in direct contact, or that the first and second features are indirect through an intermediary. touch.
  • “above”, “above” and “above” the first feature on the second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature.
  • “Below”, “beneath” and “beneath” the first feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is less horizontally than the second feature.

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Abstract

一种体外分析诊断检测装置及PCR模组，PCR模组包括第一支架(10)、升降温组件(20)与视窗组件(30)。第一支架(10)用于装设试剂卡盒(40)。升降温组件(20)包括设于第一支架(10)上的基板(21)、设于基板(21)上的半导体制冷器(22)、以及设于半导体制冷器(22)上的导热板(23)。由于试剂卡盒(40)的PCR腔室(49)有一定压力而稍微鼓起，被光学视窗(31)压缩后，PCR腔室(49)另一侧面与导热板(23)具有良好的接触，从而大大减小了材料界面之间的热传导损失，大大增加对PCR腔室(49)热循环控制的热传导效率，能较准确地控制PCR腔室(49)的温度，升降温效果明显，此外PCR模组的整体结构相对较为简单。

Description

体外分析诊断检测装置及PCR模组
相关申请的交叉引用
本公开要求于2021年10月28日提交中国专利局、申请号为2021112641764、名称为“体外分析诊断检测装置及PCR模组”，于2021年10月28日提交中国专利局、申请号为2021112641603、名称为“体外分析诊断检测装置及试剂卡盒的控温组件”，于2021年10月28日提交中国专利局、申请号为2021112641887、名称为“体外分析诊断检测装置及卡盒阀门控制组件”，于2021年10月28日提交中国专利局、申请号为2021112642199、名称为“体外分析诊断检测装置及光学检测模块”，于2021年8月27日提交中国专利局、申请号为2021109974042、名称为“体外分析诊断检测装置及PCR模组”，于2021年8月27日提交中国专利局、申请号为2021109974023、名称为“体外分析诊断检测装置及试剂卡盒的控温组件”，于2021年8月27日提交中国专利局、申请号为2021109974004、名称为“体外分析诊断检测装置及卡盒阀门控制组件”，于2021年8月27日提交中国专利局、申请号为2021109973980、名称为“体外分析诊断检测装置及光学检测模块”，的中国专利的优先权，所述专利申请的全部内容通过引用结合在本公开中。
技术领域
本公开涉及体外分析诊断技术领域，特别是涉及一种体外分析诊断检测装置及PCR模组。
背景技术
基于PCR(polymerase chain reaction，聚合酶链反应)技术的快捷检测的病原体POCT(point-of-care testing，及时检测)一体化仪器系统，该仪器系统适用于分析鼻咽拭子、痰液、尿液、粪便、宫颈/阴道拭子、血液、脑脊液、皮肤、伤口拭子等样本类型，将体液样品加入到一次性使用的试剂卡盒中，把卡盒放入该仪器系统，经过核酸扩增、检测、计算和结果打印输出，整个分析流程全自动化，未经专业培训的人员也能够轻松使用，并且获得可靠、准确的检测结果。仪器和试剂适用于感染人体的病原体DNA或RNA单个或多重检测。
在生物技术领域中使用的离心柱法或磁珠法进行核酸提取，一般需要进行裂解、结合、清洗、洗脱等四个步骤，加上后续的核酸分子杂交、聚合酶链反应和分子检测等步骤。分子检测技术朝着准确、便捷、灵敏、自动化和一体化的方向发展。然而，由于分子检测其自身技术复杂性，从样品到结果的全自动化的仪器平台极少，用于对PCR腔室内的样品液体进行升降温的结构较为复杂，且升降温效果不理想。
发明内容
基于此，有必要克服现有技术的缺陷，提供一种体外分析诊断检测装置及PCR模组，它能够使得结构简化，升降温效果较好。
其技术方案如下：一种PCR模组，所述PCR模组包括：第一支架，所述第一支架用于装设试剂卡盒；升降温组件，所述升降温组件包括设于所述第一支架上的基板、设于所述基板上的半导体制冷器、以及设于所述半导体制冷器上的导热板，所述导热板用于与所述试剂卡盒的PCR腔室的其中一侧面紧密抵触；视窗组件，所述视窗组件包括光学视窗，所述光学视窗设有至少一个透明板，所述透明板用于与所述PCR腔室的另一侧面紧密抵触。
一种体外分析诊断检测装置，所述的体外分析诊断检测装置包括所述的PCR模组。
上述的体外分析诊断检测装置及PCR模组，在工作时，将试剂卡盒装设于第一支架上，使得导热板与PCR腔室的其中一侧面紧密抵触，透明板与PCR腔室的另一侧面紧密抵触，然后半导体制冷器进行循环地升温动作与降温动作，升温时将热量通过导热板传递给PCR腔室，降温时热量通过导热板从PCR腔室传递到基板，再传递到散热片由散热片将热量向外界环境传递，从而使得PCR腔室冷却，同时由光学检测模块的激发光通过光学视窗的透明板射入到PCR腔室的样品液体，样品液体产生荧光反射回给光学检测模块进行光学检测，能对每个热循环周期的样品扩增数量进行定量检测。其中，由于试剂卡盒的PCR腔室有一定压力而稍微鼓起，被光学视窗压缩后，PCR腔室另一侧面与导热板具有良好的接触，从而大大减小了材料界面之间的热传导损失，大大增加对PCR腔室热循环控制的热传导效率，能较准确地控制PCR腔室的温度，升降温效果明显，此外PCR模组的整体结构相对较为简单。
附图说明
构成本公开的一部分的附图用来提供对本公开的进一步理解，本公开的示意性实施例及其说明用于解释本公开，并不构成对本公开的不当限定。
为了更清楚地说明本公开实施例中的技术方案，下面将对实施例描述中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是本公开的一些实施例，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图获得其他的附图。
图1为本公开一实施例的PCR模组的其中一视角结构示意图；
图2为图1中隐藏掉视窗组件与光学检测模块的其中一视角结构示意图；
图3为图1中隐藏掉视窗组件与光学检测模块的另一视角结构示意图；
图4为本公开一实施例的试剂卡盒的结构示意图；
图5为本公开一实施例的PCR模组的升降温组件的其中一视角结构图；
图6为本公开一实施例的PCR模组的升降温组件的另一视角结构图；
图7为图6在A-A处的剖视结构示意图；
图8为本公开一实施例的PCR模组的视窗组件装设于第二安装板的其中一视角结构示意图；
图9为本公开一实施例的PCR模组的视窗组件装设于第二安装板的另一视角结构示意图；
图10为图9在B处的放大结构示意图；
图11为图10在C-C处的剖视结构示意图；
图12为本公开一实施例的第一安装板的一视角结构图；
图13为本公开一实施例的第一安装板的另一视角结构图；
图14为本公开一实施例的第一安装板的又一视角结构图；
图15为图14在D-D处的剖视图；
图16为本公开一实施例的滑轨组件的一视角结构图；
图17为本公开一实施例的滑轨组件的另一视角结构图；
图18为本公开一实施例的第一支架的另一视角结构图；
图19为图18所示结构中的引导板隐藏后并在m处的放大结构示意图；
图20为本公开一实施例的温度调节件的其中一视角结构示意图；
图21为图20在n-n处的剖视结构示意图；
图22为本公开一实施例的温度调节件的另一视角结构示意图；
图23为本公开一实施例的温度调节件设有第一支架的结构示意图；
图24为图23在p-p处的剖视结构示意图；
图25为本公开一实施例的光学检测模块的其中一视角结构示意图；
图26为图25的另一视角结构示意图；
图27为图25中隐藏掉移动机构的其中一视角结构图；
图28为图25中隐藏掉移动机构的另一视角结构图；
图29为图28在Q-Q处的剖视图；
图30为本公开一实施例的移动机构的其中一视角结构图；
图31为本公开一实施例的移动机构的另一视角结构图。
10、第一支架；11、支撑板；111、第二凹部；112、进出口；113、第三凹部；114、第一安装孔；115、活动室；12、引导板；121、第一过孔；122、第二过孔；16、限位挡板；161、阻挡部；1611、导向边；162、转动件；17、第六弹性件；20、升降温组件；21、基板；22、半导体制冷器；23、导热板；24、散热件；25、第一定位板；30、视窗组件；31、光学视窗；311、透明板；312、PC板；32、第一弹性件；33、第一载板；34、镂空口；40、试剂卡盒；41、第一气孔；42、第二气孔；44、第一阀门；45、第二阀门；46、样本腔室；47、预处理腔室；48、混合腔室；49、PCR腔室；50、第一压紧组件；51、第一驱动机构；52、第一气管；521、第一接头；522、第二接头；523、第二台阶；53、第二气管；531、第三接头；532、第四接头；54、第一安装板；541、第一容纳室；542、第一导向通孔；543、第三导向通孔；544、第一台阶；55、第五弹性件；56、第一压紧件；57、第二弹性件；60、第二压紧组件；61、第二驱动机构；62、第二压紧件；63、第二安装板；631、第一凹部；64、第一限位板；70、滑轨组件；71、固定架；72、第一滑轨；73、第二滑轨；74、第一滑块；75、第二滑块；76、第一连接板；77、第二连接板；81、第一传 感器；82、第一触发件；83、第二传感器；84、第二触发件；91、热裂解组件；92、磁混合组件；100、光学检测模块；101、第二支架；1011、滑轨；1012、采光通道；10121、第一通道；10122、第二通道；102、光学检测单体；1021、光源；1022、第一透镜；1023、二向色镜；1024、第二透镜；1025、第三透镜；1026、荧光检测器；1027、第一滤镜；1028、第二滤镜；103、移动机构；1031、第三支架；10311、第三滑块；1032、第一电机；1033、第一驱动轮；1034、第二驱动轮；1035、传动元件；1041、第一传感器；1042、第一触发件；201、温度调节件；202、导热块；2021、第一导热面；2022、第二导热面；20221、限位部；2023、导热部；2024、第一凸缘；2025、第二安装孔；2026、第一安装件；203、第四弹性件；204、固定座；2041、插孔；205、温度感应器；2051、导线。
具体实施方式
为使本公开的上述目的、特征和优点能够更加明显易懂，下面结合附图对本公开的具体实施方式做详细的说明。在下面的描述中阐述了很多具体细节以便于充分理解本公开。但是本公开能够以很多不同于在此描述的其它方式来实施，本领域技术人员可以在不违背本公开内涵的情况下做类似改进，因此本公开不受下面公开的具体实施例的限制。
参阅图1至图8，图1示出了本公开一实施例的PCR模组的其中一视角结构示意图，图2与图3示出了图1中隐藏掉视窗组件30与光学检测模块100后的两个不同视角结构图，图4示出了本公开一实施例的试剂卡盒40的结构示意图，图5与图6分别示出了本公开一实施例的升降温组件20的两个不同视角结构图，图7示出了图6在A-A处的剖视结构示意图，图8示出了一实施例的PCR模组的视窗组件30装设于第二安装板63的其中一视角结构示意图。本公开一实施例提供的一种PCR模组，PCR模组包括第一支架10、升降温组件20与视窗组件30。第一支架10用于装设试剂卡盒40。升降温组件20包括设于第一支架10上的基板21、设于基板21上的半导体制冷器22、以及设于半导体制冷器22上的导热板23。导热板23用于与试剂卡盒40的PCR腔室49的其中一侧面紧密抵触。视窗组件30包括光学视窗31。光学视窗31设有至少一个透明板311。透明板311用于与PCR腔室49的另一侧面紧密抵触。
上述的PCR模组在使用时，将试剂卡盒40装设于第一支架10上，使得导热板23与PCR腔室49的其中一侧面紧密抵触，透明板311与PCR腔室49的另一侧面紧密抵触，然后半导体制冷器22进行循环地升温动作与降温动作，升温时将热量通过导热板23传递给PCR腔室49，降温时热量通过导热板23从PCR腔室49传递到基板21，再传递到散热片由散热片将热量向外界环境传递，从而使得PCR腔室49冷却，同时由光学检测模块100的激发光通过光学视窗31的透明板311射入到PCR腔室49的样品液体，样品液体产生荧光反射回给光学检测模块100进行光学检测，能对每个热循环周期的样品扩增数量进行定量检测。其中，由于试剂卡盒40的PCR腔室49有一定压力而稍微鼓起，被光学视窗31压缩后，PCR腔室49另一侧面与导热板23具有良好的接触，从而大大减小了材料界面之间的热传导损失，大大增加对PCR腔室49热循环控制的热传导效率，能较准确地控制PCR腔室49的温度，升降温效果明显，此外PCR模组的整体结构相对较为简单。
需要说明的是，本实施例中的试剂卡盒40设有至少两个腔室，流通通路、第一气孔41与第二气孔42，至少两个腔室通过流通通路连通，第一气孔41、第二气孔42均与流通通路连通，流通通路具体结构形式在此不进行限定，根据实际需求进行设置即可。
请参阅图4，具体而言，至少两个腔室例如包括样本腔室46、预处理腔室47、混合腔室48与PCR腔室49，当然还可以是其它功能腔室。样本腔室46、预处理腔室47、混合腔室48与PCR腔室49的具体数量不进行限制，根据实际需求设置即可。本实施例中，样本腔室46为一个，用于装设样本液体；预处理腔室47为一个，用于对样本液体例如加热裂解处理；混合腔室48例如为两个，用于使得样本液体与冻干试剂混合处理；PCR腔室49例如为两个，用于对样本液体进行聚合酶链反应和分子检测处理。
还需要说明的是，光学视窗31的透明板311的数量可以为一个、两个、三个或其它数量，在此不进行限定，具体根据试剂卡盒40的PCR腔室49来设置，与PCR腔室49一一对应设置。
参阅图5至图8，此外，光学视窗31包括PC板312，透明板311例如通过螺钉安装、粘设、超声焊接或激光焊接等方式固定设于PC板312上。需要说明的是，透明板311具体选用光学材料，可以与PC板312的材质相同，也可以不同，在此不进行限定。
参阅图7至图11，图9示出了本公开一实施例的PCR模组的视窗组件30装设于第二安装板63的另一视角结构示意图，图10示出了图9在B处的放大结构示意图，图11示出了图10在C-C处的剖视结构示意图。在一个实施例中，升降温组件20还包括散热件24。散热件24与基板21相连。散热件24包括叠设 于基板21上的散热板，以及与散热板相连的多个散热翅片。如此，当半导体制冷器22用于对PCR腔室49加热升温时，散热件24通过基板21将冷量较好地向外散掉；当半导体制冷器22用于对PCR腔室49降温时，散热件24通过基板21将热量较好地向外散掉。
此外，为了提高散热件24的散热效率，PCR模组还可以设置有风扇或水冷结构，通过风扇将气流吹到散热件24能快速带走散热件24的热量，或者通过水冷结构接触散热件24快速带走散热件24的热量。另外，为了使得半导体制冷器22与导热板23稳固地设置于基板21上，升降温组件20还包括第一定位板25，第一定位板25通过至少一个安装件固定地装设于基板21上，第一定位板25设有第一开口，半导体制冷器22与导热板23均设于第一开口中，第一开口的口缘与导热板23的外缘卡接连接、粘接连接、或者采用安装件固定相连。
请参阅图1至图4、图12至图15，图12至图14示出了第一安装板54的三个不同视角结构图，图15示出了图14在D-D处的剖视图。在一个实施例中，PCR模组还包括第一压紧组件50。第一压紧组件50包括第一驱动机构51、第一安装板54与至少一个第一压紧件56。第一驱动机构51设于第一支架10上，第一驱动机构51与第一安装板54相连，用于驱动第一安装板54靠近或远离于试剂卡盒40的方向移动。至少一个第一压紧件56设于第一安装板54上，第一压紧件56用于压紧或松开试剂卡盒40的第一阀门44。具体而言，第一压紧件56例如为顶针、顶杆等等，只要能实现推动第一阀门44使得第一阀门44关闭，或者松开第一阀门44时使得第一阀门44开启即可。第一压紧件56的抵触端面的尺寸大小与第一阀门44的尺寸大小相应设置。
请参阅图4、图14与图15，在一个实施例中，第一压紧组件50还包括第一气管52与第二气管53。第一气管52与第二气管53均设置于第一安装板54上，第一气管52的一端设有第一接头521，第一气管52的另一端设有第二接头522，第二气管53的一端设有第三接头531，第二气管53的另一端设有第四接头532，当第一驱动机构51驱动第一安装板54移动到第一位置时，第一接头521与第三接头531分别用于与试剂卡盒40的第一气孔41、第二气孔42对接连通。
此外，PCR模组还包括气路控制组件(图中未示意出)与压力控制机构(图中未示意出)。气路控制组件分别与第二接头522、第四接头532、压力控制机构相连通。气路控制组件设有第一工作状态与第二工作状态。当气路控制组件工作于第一工作状态时，用于控制第二接头522与压力控制机构连通，第四接头532与大气环境相通；当气路控制组件工作于第二工作状态时，用于控制第二接头522与大气环境相通，第四接头532与压力控制机构连通。
如此，当需要对试剂卡盒40内的样本液体进行检测工作时，将试剂卡盒40装入到第一支架10中，第一驱动机构51将第一气管52与第二气管53移动到第一位置，使得第一接头521与第一气孔41对接连通，第三接头531与第二气孔42对接连通；在气路控制组件工作于第一工作状态时，第一气孔41通过第一气管52与压力控制机构对接连通，第二气孔42通过第二气管53与大气环境相通，压力控制机构提供的抽吸力作用于试剂卡盒40，使得试剂卡盒40内部的样本液体在腔室之间转移；同样地，在气路控制组件工作于第二工作状态时，第二气孔42通过第二气管53与压力控制机构对接连通，第一气孔41通过第一气管52与大气环境相通，压力控制机构提供的抽吸力作用于试剂卡盒40，使得试剂卡盒40内部的样本液体在腔室之间转移。如此，通过气路控制组件进行切换动作，可以实现试剂卡盒40的第一气孔41通过第一气管52与压力控制机构连通，也可以实现试剂卡盒40的第二气孔42通过第二气管53与压力控制机构连通，能按照试剂卡盒40的工艺需求提供动力来推动样本液体在腔室之间转移，同时PCR模组的整体结构较为简化。
请参阅图4与图15，在一个实施例中，第一接头521、第三接头531均为真空吸盘。如此，第一接头521与第一气孔41对接连通时，真空吸盘完全罩住第一气孔41孔壁的外部，能保证第一接头521与第一气孔41之间的对接密封性；同样地，第三接头531与第二气孔42对接连通时，真空吸盘能完全罩住第二气孔42孔壁的外部，能保证第三接头531与第二气孔42之间的对接密封性。
此外，气路控制组件例如包括电磁控制阀与管路。电磁控制阀通过管路分别与第二接头522、第四接头532、压力控制机构相连通。压力控制机构具体为真空泵、抽风机、柱塞泵等等。电磁控制阀设有第一工作状态与第二工作状态，电磁控制阀切换工作状态时，能实现试剂卡盒40的第一气孔41通过第一气管52与压力控制机构连通，以及实现试剂卡盒40的第二气孔42通过第二气管53与压力控制机构连通。需要说明的是，电磁控制阀的设置数量，管路的具体结构，以及电磁控制阀在管路上的设置位置在此均不进行限定，根据实际需求设置，只要能实现至少上述的第一工作状态与第二工作状态即可。例如，电磁控制阀为两个，其中一个电磁控制阀动作时，能实现第二接头522通过管路与气泵相连通，或者第二接头522 通过管路与大气环境相连通，另一个电磁控制阀同步动作，能实现第四接头532通过管路与大气环境相连通，或者第四接头532通过管路与气泵相连通。
请参阅图1至图2、图8至图10，在一个实施例中，PCR模组还包括第二压紧组件60。第二压紧组件60包括第二驱动机构61与第二安装板63。第二驱动机构61设于第一支架10上，第二驱动机构61与第二安装板63相连，第二驱动机构61用于驱动第二安装板63靠近或远离于试剂卡盒40的方向移动。视窗组件30设于第二安装板63上。如此，第二驱动机构61驱动第二安装板63靠近或远离于试剂卡盒40的方向移动，第二安装板63移动时带动视窗组件30移动，能使得视窗组件30的透明板311能紧密抵触于PCR腔室49的另一侧面上，或者松开PCR腔室49的另一侧面。即当在第一支架10上进行拆装试剂卡盒40的过程中，在第二驱动机构61的驱动作用下使得透明板311远离于PCR腔室49的另一侧面，避免对试剂卡盒40造成干涉；当试剂卡盒40在第一支架10上装入到位后，在第二驱动机构61的驱动作用下使得透明板311紧密抵触于PCR腔室49的另一侧面。
需要说明的是，第一驱动机构51例如为电机丝杆驱动结构、气缸驱动结构、液压缸驱动结构、电缸驱动结构、凸轮驱动结构等等，在此不进行限定，根据实际需求进行设置即可。第二驱动机构61类似于第一驱动机构51，在此不进行赘述。
参阅图4、图8与图11，在一个实施例中，视窗组件30还包括第一弹性件32。光学视窗31通过第一弹性件32与第二安装板63相连。如此，光学视窗31的透明板311与PCR腔室49的侧面接触过程中，第一弹性件32起到缓冲作用，保证透明板311紧密接触PCR腔室49的侧面的同时，避免损坏PCR腔室49。
参阅图4、图8与图11，在一个实施例中，视窗组件30还包括设置于第一弹性件32与光学视窗31之间的第一载板33。光学视窗31设于第一载板33上，第一载板33通过第一弹性件32与第二安装板63相连。具体而言，第一载板33为铝合金等硬质材料，能加强光学视窗31的结构强度，不易于发生损坏。此外，第一弹性件32的数量在此不进行限定，例如可以是一个、两个、三个或其它数量，根据实际需求设置即可。另外，需要说明的是，为了让光学检测模块100的荧光通过光学视窗31的透明板311，第二安装板63、第一载板33、光学视窗31的PC板312上设有与透明板311位置相对应的镂空口34，该镂空口34能够让光学检测模块100的荧光通过。
参阅图4、图8与图11，在一个实施例中，第二安装板63上设有第一凹部631，第一弹性件32与第一载板33均设于第一凹部631中。第二安装板63的板面上设有第一限位板64，第一限位板64与第一载板33相抵触配合，第一限位板64绕光学视窗31的外缘周向设置。如此，一方面，在透明板311抵触PCR腔室49的侧面的过程中，第一载板33受到推力会在第一凹部631内活动，运行稳定性较好；另一方面，第一限位板64对第一载板33起到限位作用，能避免第一载板33与第一弹性件32从第一凹部631中脱离出来。
在一个实施例中，第二压紧组件60还包括至少一个第二压紧件62。至少一个第二压紧件62装设于第二安装板63上，第二压紧件62用于压紧或松开试剂卡盒40的第二阀门45。
参阅图4、图8与图12，在一个实施例中，第一安装板54上装设的第一压紧件56的数量不小于试剂卡盒40的第一阀门44的数量，第二安装板63上装设的第二压紧件62的数量不小于试剂卡盒40的第二阀门45的数量。如此，第一驱动机构51驱动第一安装板54移动时，能实现将试剂卡盒40的所有第一阀门44都打开或都关闭；同样地，第二驱动机构61驱动第二安装板63移动时，能实现将试剂卡盒40的所有第二阀门45都打开或都关闭。举例而言，本实施例中的第一压紧件56的数量为四个，其中三个第一压紧件56与试剂卡盒40的三个第一阀门44一一对应设置，另一个第一压紧件56也用于压紧或松开试剂卡盒40，但并没有压紧在设有第一阀门44的位置，用于保证对试剂卡盒40的侧面较好的压紧效果。此外，本实施例中的第二压紧件62的数量例如为四个，四个第二压紧件62与试剂卡盒40的四个第二阀门45一一对应设置。
作为一个可选的方案，例如将第一压紧组件50的第一压紧件56与第二压紧组件60的第二压紧件62集中设置在同一个安装板上，即无需设置两个安装板，通过一个驱动机构来驱动装设第一压紧件56与第二压紧件62的安装板移动即可。此外，还例如设置有第三压紧组件或第四压紧组件等等，在此不进行限定。
在一个实施例中，第一压紧组件50还包括装设于第一安装板54上的至少一个第二弹性件57。至少一个第一压紧件56与至少一个第二弹性件57一一对应设置，第一压紧件56通过第二弹性件57与第一安装板54相连。如此，在第二弹性件57的缓冲作用下，第一压紧件56接触第一阀门44过程中能避免对试剂卡盒40造成损伤。
此外，第二压紧组件60还包括装设于第二安装板63上的至少一个第三弹性件(图中未示出)，至少一个第二压紧件62与至少一个第三弹性件一一对应设置，第二压紧件62通过第三弹性件与第二安装板63相连。
参阅图15，在一个实施例中，第一安装板54内部设有第一容纳室541以及与第一容纳室541相连通的第一导向通孔542，第二弹性件57设于第一容纳室541内，第一压紧件56的一端位于第一容纳室541的内部并与第二弹性件57相连，第一压紧件56活动地设置于第一导向通孔542中，第一压紧件56的另一端伸出到第一导向通孔542的外部并用于压紧或松开试剂卡盒40的第一阀门44。
此外，第二安装板63内部设有第二容纳室以及与第二容纳室相连通的第二导向通孔(图中未示出)，第三弹性件设于第二容纳室内，第二压紧件62的一端位于第二容纳室的内部并与第三弹性件相连，第二压紧件62活动地设置于第二导向通孔中，第二压紧件62的另一端伸出到第二导向通孔的外部并用于压紧或松开试剂卡盒40的第二阀门45。
在一个实施例中，请参阅图1至图3、图16与图17，图16与图17分别示出了滑轨组件70的两个不同视角的结构示意图。在一个实施例中，PCR模组还包括滑轨组件70。滑轨组件70包括装设于第一支架10上的固定架71，设置于固定架71上的第一滑轨721011与第二滑轨731011，滑动地设置于第一滑轨721011上的第一滑块74，滑动地设置于第二滑轨731011上的第二滑块75，与第一滑块74相连的第一连接板76，以及与第二滑块75相连的第二连接板77。第一连接板76与第一安装板54相连，第二连接板77与第二安装板63相连。如此，当第一驱动机构51驱动第一安装板54移动时，第一连接板76带动第一滑块74沿着第一滑轨721011移动，这样第一安装板54的运行稳定性较好，进而能较为精准地控制第一安装板54的运动行程，即第一压紧件56的运动行程精准度达到0.05mm。此外，由于第一压紧件56的运动行程精准度较高，这样在第一阀门44开启动作时，在第一驱动机构51的动力作用下，第一压紧件56远离于第一阀门44移动距离例如0.1mm或0.2mm松开试剂卡盒40的第一阀门44，此时第一压紧件56仍然与试剂卡盒40的侧面抵触起到定位作用，使得试剂卡盒40稳定地设置于第一支架10上。
当第二驱动机构61驱动第二安装板63移动时，第二连接板77带动第二滑块75沿着第二滑轨731011移动，这样第二安装板63的运行稳定性较好，进而能较为精准地控制第二安装板63的运动行程，即第二压紧件62的运动行程精准度达到0.05mm。由于第二压紧件62的运动行程精准度较高，这样在第二阀门45开启动作时，在第二驱动机构61的动力作用下，第二压紧件62远离于第二阀门45移动距离例如0.1mm或0.2mm松开试剂卡盒40的第二阀门45，此时第二压紧件62仍然与试剂卡盒40的侧面抵触起到定位作用，使得试剂卡盒40稳定地设置于第一支架10上，此外第一接头521与第三接头531还分别保持着与试剂卡盒40的第一气孔41、第二气孔42对接连通，透明板311与PCR腔室49的另一侧面仍然处于抵触状态。
具体而言，第一安装板54、第二安装板63、第一滑块74、第二滑块75、第一连接板76与第二连接板77具体例如为铝材、铜材、铁材、不锈钢才、木材、塑料等等，在此不进行限定，根据实际需求进行设置即可。
参阅图2与图3，在一个实施例中，PCR模组还包括第一传感器104181以及与第一传感器104181感应配合的第一触发件104282。第一传感器104181装设于第一支架10上，第一触发件104282装设于第一安装板54或第一连接板76上。如此，第一传感器104181能感应到第一触发件104282的移动位置，根据感应到第一触发件104282的感应信号能得到第一安装板54的移动位置信息，进而能相应控制第一压紧件56的运动行程在预设范围内。
此外，PCR模组还包括第二传感器83以及与第二传感器83感应配合的第二触发件84。第二传感器83装设于支撑板11上，第二触发件84装设于第二安装板63或第二连接板77上。
具体而言，第一传感器104181为光电开关、接近开关、对射传感器或其它类型的传感器，只要能感应第一触发件104282的移动位置，并将第一触发件104282的移动位置信号及时反馈出来即可。第二传感器83类似于第一传感器104181，在此不再赘述。
在一个实施例中，第二压紧组件60还包括装设于第二安装板63上的至少一个第三弹性件，至少一个第二压紧件62与至少一个第三弹性件一一对应设置。第二压紧件62通过第三弹性件与第二安装板63相连。同样地，在第三弹性件的缓冲作用下，第二压紧件62接触第二阀门45过程中能避免对试剂卡盒40造成损伤。
请参阅图14与图15，在一个实施例中，第一安装板54内部设有第一容纳室541以及与第一容纳室541相连通的第一导向通孔542。第二弹性件57设于第一容纳室541内，第一压紧件56的一端位于第一 容纳室541的内部并与第二弹性件57相连，第一压紧件56活动地设置于第一导向通孔542中，第一压紧件56的另一端伸出到第一导向通孔542的外部并用于压紧或松开试剂卡盒40的第一阀门44。
第二安装板63内部设有第二容纳室(图中未示出)以及与第二容纳室相连通的第二导向通孔(图中未示出)。第三弹性件设于第二容纳室内。第二压紧件62的一端位于第二容纳室的内部并与第三弹性件相连，第二压紧件62活动地设置于第二导向通孔中，第二压紧件62的另一端伸出到第二导向通孔的外部并用于压紧或松开试剂卡盒40的第二阀门45。
在一个实施例中，第一压紧组件50还包括第五弹性件55。第一气管52通过第五弹性件55与第一安装板54相连。如此，在第一驱动机构51的推动下，第一气管52的第一接头521与试剂卡盒40的第一气孔41对接连通过程中，第五弹性件55起到缓冲作用，避免第一接头521对试剂卡盒40造成损坏。
在一个实施例中，第一安装板54上设置有第三导向通孔543，第一气管52活动地设置于第三导向通孔543中，第三导向通孔543的孔壁上设置有第一台阶544，第一气管52的外壁上设置有第二台阶523，第五弹性件55两端分别与第一台阶544、第二台阶523对应抵触。
具体而言，第五弹性件55为套设于气管的外壁上的弹簧，弹簧的两端分别与第一台阶544、第二台阶523对应抵触。
具体而言，第一弹性件32、第二弹性件57、第三弹性件、第四弹性件203与第五弹性件55均例如为弹簧、弹性块等等，在此不进行限定，根据实际需求进行设置即可。
需要说明的是，第二气管53在第一安装板54上的装设方式类似于第一气管52，在此不进行赘述。
参阅图1至图3，进一步地，第一支架10包括支撑板11与引导板12。支撑板11的其中一侧面上设有第二凹部111，第二凹部111用于与试剂卡盒40相适应。引导板12设置于支撑板11的其中一侧面上，引导板12用于抵触装入到第二凹部111中的试剂卡盒40的侧面。第二凹部111的其中一侧壁设有进出口112。如此，当需要对试剂卡盒40进行试验时，试剂卡盒40通过进出口112沿着引导板12进入到第二凹部111中，即引导板12对试剂卡盒40起到引导作用，试剂卡盒40进入到第二凹部111中后在引导板12与支撑板11的作用下位置相对稳定。
请参阅图2，在一个实施例中，PCR模组还包括热裂解组件91。热裂解组件91装设于支撑板11上，用于对试剂卡盒40的预处理腔室47内的液体进行加热裂解处理。此外，PCR模组还包括磁混合组件92，磁混合组件92装设于支撑板11上，用于使试剂卡盒40的混合腔室48内的磁珠进行上下活动，使得混合腔室48内的液体充分混合处理得到所需的混合液体。
请参阅图3、图8与图12，在一个实施例中，为了避免第一压紧件56或气管压紧于试剂卡盒40的侧面的过程中与引导板12干涉，在引导板12上开设有至少一个第一过孔121(图未示)以及至少一个第二过孔122(图未示)，至少一个第一过孔121与至少一个第一压紧件56一一对应设置，能让第一压紧件56穿过。至少一个第二过孔122与第一气管52的第一接头521对应设置，以及还与第二气管53的第三接头531对应设置，能让第一接头521穿过，以及能让第三接头531穿过。
在一个实施例中，PCR模组还包括第一传感器104181以及与第一传感器104181感应配合的第一触发件104282。第一传感器104181装设于第一支架10上，第一触发件104282装设于第一安装板54或第一连接板76上。如此，第一传感器104181能感应到第一触发件104282的移动位置，根据感应到第一触发件104282的感应信号能得到第一安装板54的移动位置信息，进而能相应控制第一压紧件56的运动行程在预设范围内。
此外，PCR模组还包括第二传感器83以及与第二传感器83感应配合的第二触发件84。第二传感器83装设于支撑板11上，第二触发件84装设于第二安装板63或第二连接板77上。
具体而言，第一传感器104181为光电开关、接近开关、对射传感器或其它类型的传感器，只要能感应第一触发件104282的移动位置，并将第一触发件104282的移动位置信号及时反馈出来即可。第二传感器83类似于第一传感器104181，在此不再赘述。
请再参阅图1至图8，在一个实施例中，一种体外分析诊断检测装置，体外分析诊断检测装置包括上述任一实施例的PCR模组。
上述的体外分析诊断检测装置在工作时，将试剂卡盒40装设于第一支架10上，使得导热板23与PCR腔室49的其中一侧面紧密抵触，透明板311与PCR腔室49的另一侧面紧密抵触，然后半导体制冷器22进行循环地升温动作与降温动作，升温时将热量通过导热板23传递给PCR腔室49，降温时热量通过导热板23从PCR腔室49传递到基板21，再传递到散热片由散热片将热量向外界环境传递，从而使得PCR腔室49冷却，同时由光学检测模块100的激发光通过光学视窗31的透明板311射入到PCR腔室49的样品液体， 样品液体产生荧光反射回给光学检测模块100进行光学检测，能对每个热循环周期的样品扩增数量进行定量检测。其中，由于试剂卡盒40的PCR腔室49有一定压力而稍微鼓起，被光学视窗31压缩后，PCR腔室49另一侧面与导热板23具有良好的接触，从而大大减小了材料界面之间的热传导损失，大大增加对PCR腔室49热循环控制的热传导效率，能较准确地控制PCR腔室49的温度，升降温效果明显，此外PCR模组的整体结构相对较为简单。
在一个实施例中，PCR模组还包括温度传感器(图中未示出)。温度传感器用于获取导热板23的温度信息。如此，通过温度传感器实时地感应导热板23的温度信息，从而便能准确地掌握导热板23的温度，并根据检测到的温度信息控制半导体制冷器22进行工作。
一般而言，安装一个温度传感器在发热体上监测发热体的温度，受限于温度传感器性能的可靠性以及生产安装流程的可靠性，实际温度和设置温度会存在一定的温差，影响样本液体的测试结果的可靠性。
进一步地，温度传感器为两个，两个温度传感器均与导热板23相连。两个温度传感器的导线2051还均用于与控制器(图中未示出)电性连接。如此，半导体制冷器22在对导热板23进行控温调节过程中，同步对比两个温度传感器返回的温度值，如若检测的两个温度值超差即报错，能确保温度传感器没有失效。此外，例如通过PID算法来控制导热板23的温度，确保导热板23温度的精准可靠。
请参阅图2、图3、图18与图19，图18示出了本公开一实施例的第一支架10的另一视角结构图，图19示出了图18所示结构中的引导板12隐藏后并在m处的放大结构示意图。在一个实施例中，PCR模组还包括限位挡板16与第六弹性件17。引导板12设置于支撑板11的其中一侧面上，引导板12用于抵触装入到第二凹部111中的试剂卡盒40的侧面。第二凹部111的其中一侧壁设有进出口112，第二凹部111的另一侧壁设有活动室115。活动室115与第二凹部111相通。限位挡板16活动地设置于活动室115内，限位挡板16通过第六弹性件17与活动室115的壁相连，限位挡板16设有阻挡部161。阻挡部161用于与装入到第二凹部111中的试剂卡盒40相抵触。如此，当需要对试剂卡盒40进行试验时，试剂卡盒40通过进出口112沿着引导板12进入到第二凹部111中，即引导板12对试剂卡盒40起到引导作用，试剂卡盒40进入到第二凹部111中后在引导板12与支撑板11的作用下位置相对稳定，能保证试剂卡盒40的相对位置较为准确，利于试剂卡盒40能对齐第一压紧件56、第二压紧件62、第一接头521、第二接头522、第三接头531、与第四接头532等部件；此外，在第六弹性件17的弹力作用下，限位挡板16的阻挡部161以一定压力大小抵触于试剂卡盒40，使得试剂卡盒40不会轻易地从进出口112向外脱离出来，即试剂卡盒40能稳固地设置于卡盒阀门控制组件，这样能保证试剂卡盒40的检测结果准确性，同时卡盒阀门控制组件的整体结构简单可靠。
另外，当试剂卡盒40的分析检测操作结束后，驱动限位挡板16移动使阻挡部161与试剂卡盒40分开，并通过进出口112将试剂卡盒40取出。
请参阅图18与图19，进一步地，限位挡板16一端通过转动件162转动地连接于活动室115的壁上。限位挡板16另一端通过第六弹性件17与活动室115的壁相连，限位挡板16另一端还设置有阻挡部161。
请参阅图18与图19，在一个实施例中，阻挡部161设有导向边1611，导向边1611朝向进出口112倾斜设置。此外，导向边1611相对于试剂卡盒40进出第二凹部111的方向的倾斜角度为a，a为30°至60°，当然a可以是0°至90°的任意角度，在此不进行限定，根据实际需求进行设置。当试剂卡盒40通过进出口112插入到第二凹部111的过程中，试剂卡盒40的凸出部接触到导向边1611，在导向边1611的导向作用下逐渐进入到第二凹部111，同时通过导向边1611推动压缩第六弹性件17；当试剂卡盒40移动到位时，阻挡部161在第六弹性件17的作用下复位移动后与试剂卡盒40的凸出部限位抵触，从而能起到卡接抵触试剂卡盒40的凸出部的作用，避免试剂卡盒40通过进出口112向外脱离出来。
需要说明的是，试剂卡盒40进出第二凹部111的方向如图19中双向箭头S所示。
在一个具体的实施例中，卡盒阀门控制组件的工作方法包括如下步骤：
步骤S10、加样。将病人样本加入到试剂卡盒40的样本腔室46内，关闭试剂卡盒40的盖子同时把试剂卡盒40插入装设于第一支架10上。限位挡板16起到限位作用，能防止试剂卡盒40从第一支架10上脱落下来。
步骤S20、在第一支架10上装设试剂卡盒40后，第一压紧组件50与第二压紧组件60工作，第一驱动机构51驱动第一安装板54靠近第一支架10移动，第二驱动机构61驱动第二安装板63靠近第一支架10移动，实现第一接头521与第一气孔41对接连通，第三接头531与第二气孔42对接连通，同时三个第一压紧件56一一对应紧密抵触于试剂卡盒40的三个第一阀门44，另一个第一压紧件56抵触于试剂卡盒40，四个第二压紧件62一一对应紧密抵触于试剂卡盒40的四个第二阀门45，使得将试剂卡盒40夹紧固 定。
步骤S30、在试剂卡盒40完全夹紧以后，控制气路控制组件工作于第一工作状态，使得第二气孔42与大气环境相通，第一气孔41与柱塞泵相连通，柱塞泵通过活塞反向运动给该第一气孔41提供一个负压，让试剂卡盒40内的样本液体从样品腔室运动到预处理腔室47。
步骤S40、样本液体转移填充满预处理腔室47后，控制第一驱动机构51移动第一安装板54使得第一安装板54带动第一压紧件56远离于试剂卡盒40预设距离，以使得试剂卡盒40的三个第一阀门44开启即可。同时控制气路控制组件工作于第二工作状态，使得第一气孔41与大气环境相通，第二气孔42与柱塞泵相连通，柱塞泵通过活塞反向运动给该第二气孔42提供一个负压。此时，裂解后的样本液体会被抽入到2个混合腔室48内，使其中的冻干试剂复溶。
步骤S50、驱动第一驱动机构51移动预设距离，使三个第一压紧件56一一对应紧密抵触于试剂卡盒40的三个第一阀门44，即关闭三个第一阀门44，并开启磁混合组件92，通过对磁混合组件92周期性通断电，使其产生周期性磁力，驱动布置于混合腔室48中磁珠上下往复运动，将复溶后的冻干试剂和样本液体混合均匀。
步骤S60、混合结束后，按照先驱动第二驱动机构61移动预设距离，使得四个第二压紧件62与四个第二阀门45分开，使得四个第二阀门45开启。通过柱塞泵中活塞的正向运动，对第二气孔42进行加压，让样品从混合腔室48流动到两个PCR腔室49中。
步骤S70、在保持一定压力的情况下，通过四个第二压紧件62紧密抵触四个第二阀门45，关闭四个第二阀门45，使试剂卡盒40的PCR腔稍微鼓起。在这四个第二阀门45关闭的同时，PCR腔室49所对应的光学视窗31也能压紧试剂卡盒40的PCR腔室49的正面。由于试剂卡盒40的PCR腔室49有一定的压力导致其稍微鼓起，使其与PCR光学视窗31贴紧，进行下一步操作。
步骤S80、第一驱动机构51与第二驱动机构61复位动作，第一压紧件56、第二压紧件62、第一接头521与第三接头531均松开试剂卡盒40，柱塞泵复位。
此外，参阅图20、图23与图24，图1示出了本公开一实施例的温度调节件201的其中一视角结构示意图，图23示出了本公开一实施例的温度调节件201设有第一支架10的结构示意图，图24示出了图23在p-p处的剖视结构示意图，在一个实施例中，体外分析诊断检测装置还包括用于给试剂卡盒40的腔室进行预调温的控温组件，控温组件包括：温度调节件201与导热块202，温度调节件201与导热块202相连，温度调节件201用于产生热量或冷量并传递给导热块202，导热块202设有第一导热面2021，第一导热面2021用于与试剂卡盒40的腔室的侧面抵触配合。
需要说明的是，该腔室既可以是样本液体的预处理腔室47，又可以是样本液体的PCR腔室49等等，在此不进行限定。
当用于对试剂卡盒40的腔室内的样本液体进行预升温或预降温时，使得第一导热面2021与试剂卡盒40的腔室的侧面紧密抵触配合，温度调节件201工作，温度调节件201将热量或冷量传递给导热块202，通过导热块202的第一导热面2021将热量或冷量传递给腔室的侧面。相对于传统的从腔室的底部对腔室内的样本液体进行升降温方式而言，采取第一导热面2021与腔室的侧面抵触配合来传递能量的方式，能使得第一导热面2021的能量较好地传递给腔室内的样本液体，且样本液体受热较为均匀，能达到预期的加热效果或制冷效果，能保证检测结果准确可靠。
需要说明的是，本实施例中的试剂卡盒40具体包括板件与分别位于板件两个相对侧面上的两个薄膜，板件设有窗口，两个薄膜与窗口围合形成用于装设样本液体的腔室。窗口的开口面尺寸大小远远大于板件的两个相对侧面之间的间距，窗口的口径尺寸与两个相对侧面的间距尺寸的比例例如为5：1、10：1、20：1或其它比例关系，这样当第一导热面2021与腔室的侧面抵触配合来传递能量时，便能实现将第一导热面2021的能量较好地传递给腔室内的样本液体，且样本液体受热较为均匀，能达到预期的加热效果或制冷效果，能保证检测结果准确可靠。
具体而言，导热块202具体例如为一个并设置抵触于试剂卡盒40的腔室的其中一个侧面；此外，导热块202也可以例如为两个，并分别对应设置抵触于试剂卡盒40的腔室的两个侧面上，即对试剂卡盒40的腔室的两个侧面均同步进行加热或制冷。
此外，本实施例中，导热块202具体例如为金属块，金属块可以是铝块、铜块等等。当然，导热块202不限于是金属块，也可以选用其它导热性能较好的非金属材料，根据实际需求进行设置。导热块202作为温度调节件201与试剂卡盒40的腔室的中间元件，能实现将温度调节件201产生的热量或冷量较为均匀地传递给腔室的侧面。
在一个实施例中，第一导热面2021的形状用于与试剂卡盒40的腔室的侧面的形状相适应。作为一个示例，当腔室的侧面的形状为圆形、椭圆形、方形、六边形时，第一导热面2021的形状相应为圆形、椭圆形、方形、六边形时。
作为一个示例，第一导热面2021能够覆盖试剂卡盒40的腔室的侧面。如此，第一导热面2021与腔室的侧面抵触配合时，能保证腔室的侧面均与第一导热面2021相接触，这样第一导热面2021便能将热量均匀地传递给腔室的侧面，使得样本液体受热较为均匀，能达到预期的加热效果或制冷效果，能保证检测结果准确可靠。
请参阅图20至图22，图21示出了图20在n-n处的剖视结构示意图，图22示出了本公开一实施例的温度调节件201的另一视角结构示意图。在一个实施例中，PCR模组还包括第四弹性件203与固定座204。导热块202还设有与第一导热面2021相对设置的第二导热面2022。温度调节件201的其中一侧面与第二导热面2022相互抵触，温度调节件201的另一侧面通过第四弹性件203与固定座204相连。如此，在第四弹性件203的作用下，一方面能实现导热块202以一定压力地紧密抵触于腔室的侧面，从而能较好地将热量或冷量传递给腔室内的样本液体，样本液体受热较为均匀，能达到预期的加热效果或制冷效果，能保证检测结果准确可靠；另一方面，在第四弹性件203的作用下，导热块202对腔室的侧面处的薄膜的压紧力不至于过大而导致薄膜出现损坏。
作为一个示例，第四弹性件203例如可以是弹簧、弹性块等等，在此不进行限定，根据实际需求进行设置即可。
作为一个示例，第四弹性件203的一端与温度调节件201相连或相抵触，第四弹性件203的另一端与固定座204相连或相抵触。当第四弹性件203的一端与温度调节件201相抵触时，具体与温度调节件201的侧面的中部部位相抵触；此外，当第四弹性件203的另一端与固定座204相抵触时，在固定座204上设置有插孔2041(如图2所示)，第四弹性件203的另一端伸入到插孔2041与插孔2041的孔壁相抵触。
请参阅图23与图24，在一个实施例中，支撑板11设置有由支撑板11的其中一侧面延伸到另一侧面的第一安装孔114。导热块202活动地设置于第一安装孔114中。固定座204设置于支撑板11的其中一侧面(如图24所示的右侧面)上，第一导热面2021凸出到支撑板11的另一侧面(如图24所示的左侧面)的外部。如此，将试剂卡盒40装设于支撑板11的另一侧面上，当试剂卡盒40与支撑板11的另一侧面相抵触固定位置时，由于第一导热面2021凸出到支撑板11的另一侧面的外部，即第一导热面2021伸出到第一安装孔114的外部，这样第一导热面2021能便于与试剂卡盒40的腔室的侧面紧密抵触配合。
需要说明的是，导热块202设置于第一安装孔114的口壁上时，第一导热面2021凸出到支撑板11的另一侧面外部的高度值例如为0.5mm、1mm、2mm或5mm等等。
请参阅图21与图24，进一步地，导热块202包括导热部2023以及绕导热部2023周向设置的第一凸缘2024。第一凸缘2024与支撑板11的其中一侧面抵触配合，导热部2023活动地设置于第一安装孔114中，第一导热面2021设置于导热部2023上。如此，在支撑板11的另一侧面未装设试剂卡盒40时，在第四弹性件203的作用下，第四弹性件203使得第一凸缘2024与支撑板11的其中一侧面紧密抵触，第一凸缘2024起到限制导热块202的位置的作用，此时导热部2023的第一导热面2021凸出到支撑板11的另一侧面外部的高度最大。
具体而言，导热部2023与第一安装孔114相适应设置，这样导热部2023沿着第一安装孔114移动时稳定性较好。导热部2023例如为圆柱状、方形柱体状等等，第一安装孔114相应为圆形通孔、方形通孔等等。
当然，作为一个示例，也可以无需设置第一凸缘2024，例如第四弹性件203与导热块202相连时通过第四弹性件203来限制导热块202的位置。
需要说明的是，该“第一凸缘2024”可以为“导热部2023的一部分”，即“第一凸缘2024”与“导热部2023的其他部分”一体成型制造；也可以与“导热部2023的其他部分”可分离的一个独立的构件，即“第一凸缘2024”可以独立制造，再与“导热部2023的其他部分”组合成一个整体。如图21所示，一实施例中，“第一凸缘2024”为“导热部2023”一体成型制造的一部分。
参阅图24，进一步地，支撑板11的其中一侧面上设置有与第一凸缘2024相适应的第三凹部113。第一凸缘2024设置于第三凹部113中并与第三凹部113的底壁相抵触配合。第一安装孔114由第三凹部113的底壁延伸到支撑板11的另一侧面。如此，将导热块202的第一凸缘2024设置于第三凹部113中后，第三凹部113对第一凸缘2024起到限位作用，这样导热块202受力活动时运行较为稳定。此外，将第一凸缘2024设置于第三凹部113中时，便能实现将导热部2023快速地装设于第一安装孔114中，导热块202 在支撑板11上的安装操作较为方便。
请参阅图24，进一步地，支撑板11的另一侧面上设置有用于容纳试剂卡盒40的第二凹部111。第一安装孔114从支撑板11的其中一侧面延伸到第二凹部111的底壁。第一支架10还包括装设于支撑板11的另一侧面上的引导板12，引导板12用于与试剂卡盒40背向于导热块202的侧面抵触配合。如此，将试剂卡盒40装设于第二凹部111中后，由于引导板12与试剂卡盒40背向于导热块202的侧面抵触固定，试剂卡盒40的另一侧面与第二凹部111的底壁相抵触，即能实现将试剂卡盒40稳定地固定于第二凹部111中；此外，在试剂卡盒40稳固于第二凹部111中的基础上，导热块202的第一导热面2021能稳定地紧密抵触于腔室的侧面，使得热量或冷量的传递效果较好。
需要说明的是，该“引导板12”可以为“支撑板11的一部分”，即“引导板12”与“支撑板11的其他部分”一体成型制造；也可以与“支撑板11的其他部分”可分离的一个独立的构件，即“引导板12”可以独立制造，再与“支撑板11的其他部分”组合成一个整体。
需要说明的是，作为一个可选的方案，也可以无需在支撑板11的另一侧面上设置引导板12，而是在试剂卡盒40的两个相对侧面的位置处均设置导热块202，也就是通过两个导热块202分别与试剂卡盒40的两个相对侧面同步进行抵触来实现传递热量或冷量。
请参阅图21，在一个实施例中，体外分析诊断检测装置还包括温度感应器205。温度感应器205用于获取导热块202的温度信息。如此，通过温度感应器205实时地感应导热块202的温度信息，从而便能准确地掌握导热块202的温度，并根据检测到的温度信息控制温度调节件201进行工作。
一般而言，安装一个温度感应器205在发热体上监测发热体的温度，受限于温度感应器205性能的可靠性以及生产安装流程的可靠性，实际温度和设置温度会存在一定的温差，影响样本液体的测试结果的可靠性。
请参阅图20至图22，进一步地，温度感应器205为两个，两个温度感应器205均与导热块202相连。两个温度感应器205的导线2051还均用于与控制器(图中未示出)电性连接。如此，温度调节件201在对导热块202进行控温调节过程中，同步对比两个温度感应器205返回的温度值，如若检测的两个温度值超差即报错，能确保温度感应器205没有失效。此外，例如通过PID算法来控制导热块202的温度，确保导热块202温度的精准可靠。
参阅图21，在一个实施例中，导热块202上设有第二安装孔2025。温度感应器205设置于第二安装孔2025的孔壁上，温度感应器205的导线2051经第二安装孔2025向外伸出并用于与控制器电性连接；第二安装孔2025内部填充有导热胶。
具体而言，第二安装孔2025例如为盲孔，将温度感应器205设置于盲孔内后，通过导热胶填充于盲孔中。如此，温度感应器205固定于导热块202的盲孔内，导热胶利于将热量传递给温度感应器205，能较灵敏地感应到导热块202的温度信息。当然，第二安装孔2025也可以是通孔，温度感应器205装设于第二安装孔2025后，采用导热胶填充于第二安装孔2025。
请参阅图21与图24，在一个实施例中，导热块202设置有与第一导热面2021相对设置的第二导热面2022，温度调节件201为功率电阻，功率电阻的其中一侧面与第二导热面2022相贴合。功率电阻通过第一安装件2026固定地装设于导热块202上。具体而言，第一安装件2026例如为螺钉、销钉、铆钉、卡接件等等。当然，功率电阻也可以采用导热粘胶粘接固定于导热块202上。
请参阅图21与图22，在一个实施例中，功率电阻的其中一侧面通过相变导热贴纸与第二导热面2022相贴合。如此，功率电阻产生的热量能较好地传递给导热块202上。
请参阅图21与图22，可选地，第二导热面2022上设有间隔的若干个限位部20221。若干个限位部20221均与功率电阻的边缘相抵触。若干个限位部20221均与功率电阻的边缘抵触，能使得功率电阻与导热块202两者稳固地结合在一起。
在另一个实施例中，导热块202设置有与第一导热面2021相对设置的第二导热面2022。温度调节件201包括帕尔贴(图中未示意出)与散热器件(图中未示意出)。帕尔贴的其中一侧面与第二导热面2022相贴合，帕尔贴的另一侧面与散热器件相贴合。导热块202、帕尔贴与散热器件三者通过第二安装件(图中未示意出)固定相连。如此，帕尔贴既可以产生热量并将热量传递给导热块202，起到制热作用；又可以产生冷量并将冷量传递给导热块202，起到制冷作用。
在一个实施例中，请参阅图1至图5，在一个实施例中，体外分析诊断检测装置还包括光学检测模块100。具体而言，光学检测模块100包括第二支架101、多个光学检测单体102、以及移动机构103。多个光学检测单体102依次间隔地设置于第二支架101上，光学检测单体102用于对PCR腔室49内的样品液 体进行荧光检测。移动机构103与第二支架101相连，移动机构103用于驱动第二支架101移动，以使得多个光学检测单体102依次移动到与PCR腔室49相对的位置。
上述的光学检测模块100，当需要对PCR腔室49进行荧光检测时，通过移动机构103驱动第二支架101移动，使多个光学检测单体102依次移动到与PCR腔室49相对的位置，并依次对PCR腔室49进行荧光检测。相对于传统的光学检测结构而言，一方面，驱动多个光学检测单体102沿着竖向方向或水平方向依次移动到与PCR腔室49相对的位置进行荧光检测的方式，切换速度较快，较为方便，能够提高荧光检测效率；另一方面，光学检测模块100的整体结构相对简单，且多个光学检测单体102相互独立，能便于进行维护工作，使用寿命较长。
参阅图25至图31，在一个实施例中，多个光学检测单体102沿着第一方向依次间隔地设置于第二支架101上，移动机构103用于驱动第二支架101沿着第一方向移动。第一方向为竖向方向、水平方向或与竖向方向设有夹角的方向。第一方向具体例如图28中的f所示。需要说明的是，第一方向为与竖向方向设有夹角的方向即为相对于竖向方向倾斜设置的方向。
请参阅图30与图31，图30与图31分别示出了一实施例移动机构103的两个不同视角结构图。在一个实施例中，移动机构103包括第三支架1031、第一电机1032、第一驱动轮1033、第二驱动轮1034、连接第一驱动轮1033与第二驱动轮1034的传动元件1035。第一电机1032、第一驱动轮1033与第二驱动轮1034均设于第三支架1031上、第一电机1032的转轴与第一驱动轮1033相连，用于驱动第一驱动轮1033转动。传动元件1035还与第二支架101相连。传动元件1035与第二支架101的连接包括但不限于卡接相连，粘接相连，或采用螺钉、销钉、绳索等安装件连接在一起，这样传动元件1035移动时能带动第二支架101移动。第二支架101滑动地设置于第三支架1031上。其中，第一驱动轮1033、第二驱动轮1034具体例如为齿轮、链轮、皮带轮等，传动元件1035与第一驱动轮1033、第二驱动轮1034相应设置。如此，第一电机1032工作时，第一驱动轮1033带动传动元件1035移动，传动元件1035移动时带动第二支架101运行，以使得带动不同的光学检测单体102次移动到与PCR腔室49相对的位置。此外，由于第二支架101滑动地设置于第三支架1031上，即第二支架101运行时沿着第三支架1031滑动，运行效果较为稳定可靠。作为一个示例，第二支架101上设置有第三滑轨1011，第三支架1031上设置有与第三滑轨1011滑动配合的第三滑块10311。
作为一个示例，移动机构103不限于是上述的第一电机1032、第一驱动轮1033、第二驱动轮1034与传动元件1035的组合形式，还可以例如为电机丝杆驱动结构，油缸驱动机构，电缸驱动机构，气缸驱动机构等等，在此不进行限定。
请参阅图4与图29，在一个实施例中，光学检测模块100还包括第一传感器104181(如图25所示)以及与第一传感器104181感应配合的第一触发件104282。第一传感器104181装设于第二支架101上，第一触发件104282装设于第三支架1031上。如此，第一传感器104181能感应到第一触发件104282的移动位置，根据感应到第一触发件104282的感应信号能得到第二支架101的移动位置信息，进而能相应控制多个光学检测单体102能较为精准地移动到与PCR腔室49相对的位置。
具体而言，第一传感器104181为光电开关、接近开关、对射传感器或其它类型的传感器，只要能感应第一触发件104282的移动位置，并将第一触发件104282的移动位置信号及时反馈出来即可。
请再参阅图4与图29，在一个实施例中，多个光学检测单体102均包括光源1021。多个光学检测单体102的光源1021为不同波段的光源1021。如此，光学检测单体102产生的荧光的波段根据光源1021的波段来定，多个不同波段的光源1021对应于多种不同波段的荧光，即能提供不同波段的荧光来对PCR腔室49的样品液体进行荧光检测，保证荧光检测效果与荧光检测效率，同时满足不同检测项目的需求。本实施例中，光学检测单体102具体例如为5个，相应地产生5种不同波段的荧光。5个荧光的具体波段大小根据实际需求进行设置，在此不进行限定。此外，光学检测单体102也可以是其它数量，例如为3个、4个、6个、8个等等，在此不进行限定。
作为一个可选的方案，多个光学检测单体102中，可以存在其中两个、三个或其它数量的光学检测单体102提供的光源1021的波段相同。当然，多个光学检测单体102的光源1021也可以是完全互不相同的波段。
请再参阅图4与图29，在一个实施例中，第二支架101上设有与多个光学检测单体102一一对应设置的多个采光通道1012。光学检测单体102对应地设置于采光通道1012中。采光通道1012包括第一通道10121与第二通道10122。第二通道10122与第一通道10121的中部部位垂直连通，光源1021设于第二通道10122远离于第一通道10121的端部上。光学检测单体102还包括第一透镜1022、二向色镜1023、第 二透镜1024、第三透镜1025与荧光检测器1026，第一透镜1022设于第二通道10122的内壁，二向色镜1023倾斜地设于第一通道10121的中部部位，第二透镜1024、二向色镜1023、第三透镜1025与荧光检测器1026依次地设于第一通道10121的内壁，第二透镜1024位于第一通道10121靠近于PCR腔室49的一端，荧光检测器1026位于第一通道10121远离于PCR腔室49的一端。具体而言，光源1021具体为LED灯。此外，荧光检测器1026具体为光电二极管检测器。
在一个实施例中，光学检测单体102还包括第一滤镜1027与第二滤镜1028。第一滤镜1027设于第二通道10122的内壁，第一滤镜1027位于第一透镜1022与二向色镜1023之间。第二滤镜1028设于第一通道10121的内壁，第二滤镜1028位于第二透镜1024与二向色镜1023之间。
请再参阅图4与图29，以其中一个光学检测单体102为例对光学检测单体102的工作方法进行说明，光源1021产生激发光，激发光经过第一透镜1022准直处理，准直后的激发光经过第一滤镜1027时由第一滤镜1027过滤掉杂光，过滤掉杂光的激发光由二向色镜1023反射进入第二透镜1024，由第二透镜1024将激发光汇聚照射到试剂卡盒40的PCR腔的样品液体上；某一温度状态下的样品液体产生荧光，荧光由第二透镜1024进行收集，并经由二向色镜1023进入到第二滤镜1028，第二滤镜1028对荧光过滤处理后进入到第三透镜1025，通过第三透镜1025将荧光汇聚到荧光检测器1026，进行荧光信号的检测。
请参阅图4、图10、图11、图25、图28与图29，在一个实施例中，以试剂卡盒40的PCR腔室49为两个，以及光学检测单体102为五个作为示例，来对本实施例中的PCR腔室49的光学检测方法进行详细介绍，上述步骤S70具体包括如下步骤：
步骤S710、在样本液体从混合腔室48流到两个PCR腔室49后，保持一定压力的情况下，第二驱动机构61带动安装板朝靠近试剂卡盒40的方向移动，使压紧件压紧于试剂卡盒40的阀门上，试剂卡盒40的PCR腔室49呈稍微鼓起状态。光学视窗31也同步压紧于PCR腔室49的正面。由于PCR腔室49有一定的压力导致其稍微鼓起，在其被光学视窗31压缩后，PCR腔室49的背面与导热板23具有良好的接触，减小了材料界面之间的热传导损失，大大增加对PCR腔室49热循环控制的热传导效率。
步骤S720、升降温组件20开始进行热循环工作，例如先使得PCR腔室49的样本液体升温到第一预设温度，然后又使得PCR腔室49的样本液体降温到第二预设温度，并按照设定程序不断循环。第一预设温度、第二预设温度根据实际情况设置，在此不进行限定。
步骤S730、在升降温组件20对PCR腔室49的样本液体进行热循环的同时，5个光学检测单体102(依次记为第一光学检测单体102、第二光学检测、第三光学检测单体102、第四光学检测单体102、第五光学检测单体102)通过移动机构103的控制进行上下往复运动，其过程中停留6个位置，每个停留位置不同的光学检测单体102会依次与试剂卡盒40上下两个PCR腔对齐。同时，光学检测单体102在每个位置停留期间LED电路板上光源1021依次打开，荧光检测器1026进行光学信号采集。光学模块采集时序分别为：第一光学检测单体102对准上PCR腔室49→第二光学检测单体102对准上PCR腔室49、第一光学检测单体102对准下PCR腔室49→第三光学检测单体102对准上PCR腔室49、第二光学检测单体102对准下PCR腔室49→第四光学检测单体102对准上PCR腔室49、第三光学检测单体102对准下PCR腔室49→第五光学检测单体102对准上PCR腔室49、第四光学检测单体102对准下PCR腔室49→第五光学检测单体102对准下PCR腔室49。这个过程为一个周期。一个周期结束后，光学检测模块100回到初始位置，即第一光学检测单体102对准上PCR腔室49，进入到第二个周期。如此反复，从而对每个热循环周期的样品扩增数量进行定量检测。
步骤S740、热循环以及光学采样结束后，光学检测模块100回到初始位置，第二驱动机构61驱动安装板朝远离于第一支架10的方向移动，从第一支架10中取出试剂卡盒40，完成一次对核酸的扩增和检测。
以上实施例的各技术特征可以进行任意的组合，为使描述简洁，未对上述实施例中的各个技术特征所有可能的组合都进行描述，然而，只要这些技术特征的组合不存在矛盾，都应当认为是本说明书记载的范围。
以上所述实施例仅表达了本公开的几种实施方式，其描述较为具体和详细，但并不能因此而理解为对公开专利范围的限制。应当指出的是，对于本领域的普通技术人员来说，在不脱离本公开构思的前提下，还可以做出若干变形和改进，这些都属于本公开的保护范围。因此，本公开专利的保护范围应以所附权利要求为准。
在本公开的描述中，需要理解的是，术语“中心”、“纵向”、“横向”、“长度”、“宽度”、“厚度”、“上”、“下”、“前”、“后”、“左”、“右”、“竖直”、“水平”、“顶”、“底”、“内”、“外”、“顺时针”、“逆时针”、“轴向”、“径向”、“周向”等指示的方位或位置关系为基于附图 所示的方位或位置关系，仅是为了便于描述本公开和简化描述，而不是指示或暗示所指的装置或元件必须具有特定的方位、以特定的方位构造和操作，因此不能理解为对本公开的限制。
此外，术语“第一”、“第二”仅用于描述目的，而不能理解为指示或暗示相对重要性或者隐含指明所指示的技术特征的数量。由此，限定有“第一”、“第二”的特征可以明示或者隐含地包括至少一个该特征。在本公开的描述中，“多个”的含义是至少两个，例如两个，三个等，除非另有明确具体的限定。
在本公开中，除非另有明确的规定和限定，术语“安装”、“相连”、“连接”、“固定”等术语应做广义理解，例如，可以是固定连接，也可以是可拆卸连接，或成一体；可以是机械连接，也可以是电连接；可以是直接相连，也可以通过中间媒介间接相连，可以是两个元件内部的连通或两个元件的相互作用关系，除非另有明确的限定。对于本领域的普通技术人员而言，可以根据具体情况理解上述术语在本公开中的具体含义。
在本公开中，除非另有明确的规定和限定，第一特征在第二特征“上”或“下”可以是第一和第二特征直接接触，或第一和第二特征通过中间媒介间接接触。而且，第一特征在第二特征“之上”、“上方”和“上面”可是第一特征在第二特征正上方或斜上方，或仅仅表示第一特征水平高度高于第二特征。第一特征在第二特征“之下”、“下方”和“下面”可以是第一特征在第二特征正下方或斜下方，或仅仅表示第一特征水平高度小于第二特征。
需要说明的是，当元件被称为“固定于”或“设置于”另一个元件，它可以直接在另一个元件上或者也可以存在居中的元件。当一个元件被认为是“连接”另一个元件，它可以是直接连接到另一个元件或者可能同时存在居中元件。本文所使用的术语“垂直的”、“水平的”、“上”、“下”、“左”、“右”以及类似的表述只是为了说明的目的，并不表示是唯一的实施方式。

Claims (34)

  1. 一种PCR模组，所述PCR模组包括：
    第一支架，所述第一支架用于装设试剂卡盒；
    升降温组件，所述升降温组件包括设于所述第一支架上的基板、设于所述基板上的半导体制冷器、以及设于所述半导体制冷器上的导热板，所述导热板用于与所述试剂卡盒的PCR腔室的其中一侧面紧密抵触；
    视窗组件，所述视窗组件包括光学视窗，所述光学视窗设有至少一个透明板，所述透明板用于与所述PCR腔室的另一侧面紧密抵触。
  2. 根据权利要求1所述的PCR模组，其中，所述升降温组件还包括散热件，所述散热件与所述基板相连；所述散热件包括叠设于所述基板上的散热板，以及与所述散热板相连的多个散热翅片。
  3. 根据权利要求1所述的PCR模组，其中，所述PCR模组还包括第一压紧组件，所述第一压紧组件包括第一驱动机构、第一安装板与至少一个第一压紧件，所述第一驱动机构设于所述第一支架上，所述第一驱动机构与所述第一安装板相连，用于驱动所述第一安装板靠近或远离于所述试剂卡盒的方向移动；至少一个所述第一压紧件设于所述第一安装板上，所述第一压紧件用于压紧或松开所述试剂卡盒的第一阀门。
  4. 根据权利要求3所述的PCR模组，其中，所述第一压紧组件还包括第一气管与第二气管，所述第一气管与所述第二气管均设置于所述第一安装板上，所述第一气管的一端设有第一接头，所述第一气管的另一端设有第二接头，所述第二气管的一端设有第三接头，所述第二气管的另一端设有第四接头，当所述第一驱动机构驱动所述第一安装板移动到第一位置时，所述第一接头与所述第三接头分别用于与所述试剂卡盒的第一气孔、第二气孔对接连通；
    所述PCR模组还包括气路控制组件与压力控制机构，所述气路控制组件分别与所述第二接头、所述第四接头、所述压力控制机构相连通；所述气路控制组件设有第一工作状态与第二工作状态；当所述气路控制组件工作于所述第一工作状态时，用于控制所述第二接头与所述压力控制机构连通，所述第四接头与大气环境相通；当所述气路控制组件工作于所述第二工作状态时，用于控制所述第二接头与大气环境相通，所述第四接头与所述压力控制机构连通。
  5. 根据权利要求3所述的PCR模组，其中，所述PCR模组还包括第二压紧组件；所述第二压紧组件包括第二驱动机构与第二安装板；所述第二驱动机构设于所述第一支架上，所述第二驱动机构与所述第二安装板相连，用于驱动所述第二安装板靠近或远离于所述试剂卡盒的方向移动；所述视窗组件设于所述第二安装板上。
  6. 根据权利要求5所述的PCR模组，其中，所述视窗组件还包括第一弹性件；所述光学视窗通过所述第一弹性件与所述第二安装板相连。
  7. 根据权利要求6所述的PCR模组，其中，所述视窗组件还包括设置于所述第一弹性件与所述光学视窗之间的第一载板，所述光学视窗设于所述第一载板上，所述第一载板通过所述第一弹性件与所述第二安装板相连。
  8. 根据权利要求7所述的PCR模组，其中，所述第二安装板上设有第一凹部，所述第一弹性件与所述第一载板均设于所述第一凹部中；所述第二安装板的板面上设有第一限位板，所述第一限位板与所述第一载板相抵触配合，所述第一限位板绕所述光学视窗的外缘周向设置。
  9. 根据权利要求5所述的PCR模组，其中，所述第二压紧组件还包括至少一个第二压紧件；至少一个所述第二压紧件装设于所述第二安装板上，所述第二压紧件用于压紧或松开所述试剂卡盒的第二阀门。
  10. 根据权利要求9所述的PCR模组，其中，所述第一压紧组件还包括装设于所述第一安装板上的至少一个第二弹性件，至少一个所述第一压紧件与至少一个所述第二弹性件一一对应设置，所述第一压紧件通过所述第二弹性件与所述第一安装板相连；
    所述第二压紧组件还包括装设于所述第二安装板上的至少一个第三弹性件，至少一个所述第二压紧件与至少一个所述第三弹性件一一对应设置，所述第二压紧件通过所述第三弹性件与所述第二安装板相连。
  11. 根据权利要求10所述的PCR模组，其中，所述第一安装板内部设有第一容纳室以及与所述第一容纳室相连通的第一导向通孔，所述第二弹性件设于所述第一容纳室内，所述第一压紧件的一端位于所述第 一容纳室的内部并与所述第二弹性件相连，所述第一压紧件活动地设置于所述第一导向通孔中，所述第一压紧件的另一端伸出到所述第一导向通孔的外部并用于压紧或松开所述试剂卡盒的第一阀门；
    所述第二安装板内部设有第二容纳室以及与所述第二容纳室相连通的第二导向通孔，所述第三弹性件设于所述第二容纳室内，所述第二压紧件的一端位于所述第二容纳室的内部并与所述第三弹性件相连，所述第二压紧件活动地设置于所述第二导向通孔中，所述第二压紧件的另一端伸出到所述第二导向通孔的外部并用于压紧或松开所述试剂卡盒的第二阀门。
  12. 根据权利要求5所述的PCR模组，其中，所述PCR模组还包括滑轨组件；所述滑轨组件包括装设于所述第一支架上的固定架，设置于所述固定架上的第一滑轨与第二滑轨，滑动地设置于所述第一滑轨上的第一滑块，滑动地设置于所述第二滑轨上的第二滑块，与所述第一滑块相连的第一连接板，以及与所述第二滑块相连的第二连接板；所述第一连接板与所述第一安装板相连，所述第二连接板与所述第二安装板相连。
  13. 根据权利要求12所述的PCR模组，其中，所述PCR模组还包括第一传感器以及与所述第一传感器感应配合的第一触发件；所述第一传感器装设于所述第一支架上，所述第一触发件装设于所述第一安装板或所述第一连接板上；所述PCR模组还包括第二传感器以及与所述第二传感器感应配合的第二触发件；所述第二传感器装设于所述第一支架上，所述第二触发件装设于所述第二安装板或所述第二连接板上。
  14. 一种体外分析诊断检测装置，所述的体外分析诊断检测装置包括如权利要求1至13任意一项所述的PCR模组。
  15. 根据权利要求14所述的体外分析诊断检测装置，其中，所述体外分析诊断检测装置还包括用于给所述试剂卡盒的腔室进行预调温的控温组件，所述控温组件包括：温度调节件与导热块，所述温度调节件与所述导热块相连，所述温度调节件用于产生热量或冷量并传递给所述导热块，所述导热块设有第一导热面，所述第一导热面用于与试剂卡盒的腔室的侧面抵触配合。
  16. 根据权利要求15所述的体外分析诊断检测装置，其中，所述第一导热面的形状与所述试剂卡盒的腔室的侧面的形状相适应；或者所述第一导热面能够覆盖所述试剂卡盒的腔室的侧面。
  17. 根据权利要求15所述的体外分析诊断检测装置，其中，所述体外分析诊断检测装置还包括第四弹性件与固定座；所述导热块还设有与所述第一导热面相对设置的第二导热面；所述温度调节件通过所述第四弹性件与所述固定座相连。
  18. 根据权利要求17所述的体外分析诊断检测装置，其中，所述第一支架包括支撑板，所述支撑板设置有由所述支撑板的其中一侧面延伸到另一侧面的第一安装孔；所述导热块活动地设置于所述第一安装孔中，所述固定座设置于所述支撑板的其中一侧面上，所述第一导热面凸出到所述支撑板的另一侧面的外部。
  19. 根据权利要求18所述的体外分析诊断检测装置，其中，所述支撑板的另一侧面上设置有用于容纳所述试剂卡盒的第二凹部，所述第一安装孔从所述支撑板的其中一侧面延伸到所述第二凹部的底壁；所述第一支架还包括装设于所述支撑板的另一侧面上的引导板，所述引导板与所述试剂卡盒背向于所述导热块的侧面抵触配合。
  20. 根据权利要求18所述的体外分析诊断检测装置，其中，所述导热块包括导热部以及绕所述导热部周向设置的第一凸缘；所述第一凸缘与所述支撑板的其中一侧面抵触配合，所述导热部活动地设置于所述第一安装孔中，所述第一导热面设置于所述导热部上。
  21. 根据权利要求20所述的体外分析诊断检测装置，其中，所述支撑板的其中一侧面上设置有与所述第一凸缘相适应的第三凹部，所述第一凸缘设置于所述第三凹部中并与所述第三凹部的底壁相抵触配合；所述第一安装孔由所述第三凹部的底壁延伸到所述支撑板的另一侧面。
  22. 根据权利要求15所述的体外分析诊断检测装置，其中，所述体外分析诊断检测装置还包括温度感应器；所述温度感应器用于获取所述导热块的温度信息。
  23. 根据权利要求22所述的体外分析诊断检测装置，其中，所述温度感应器为两个，两个所述温度感应器均与所述导热块相连，两个所述温度感应器的导线还均用于与控制器电性连接。
  24. 根据权利要求22所述的体外分析诊断检测装置，其中，所述导热块上设有第二安装孔，所述温度感应器设置于所述第二安装孔的孔壁上，所述温度感应器的导线经所述第二安装孔向外伸出并用于与控制器电性连接；所述第二安装孔内部填充有导热胶。
  25. 根据权利要求15所述的体外分析诊断检测装置，其中，所述导热块设置有与所述第一导热面相对设置的第二导热面，所述温度调节件为功率电阻，所述功率电阻的其中一侧面与所述第二导热面相贴合；所述功率电阻通过第一安装件固定地装设于所述导热块上。
  26. 根据权利要求25所述的体外分析诊断检测装置，其中，所述功率电阻的其中一侧面通过相变导热贴纸与所述第二导热面相贴合；所述第二导热面上设有间隔的若干个限位部，若干个所述限位部均与所述功率电阻的边缘相抵触。
  27. 根据权利要求15所述的体外分析诊断检测装置，其中，所述导热块设置有与所述第一导热面相对设置的第二导热面，所述温度调节件包括帕尔贴与散热器件；所述帕尔贴的其中一侧面与所述第二导热面相贴合，所述帕尔贴的另一侧面与所述散热器件相贴合；所述导热块、所述帕尔贴与所述散热器件三者通过第二安装件固定相连。
  28. 根据权利要求14所述的体外分析诊断检测装置，其中，所述体外分析诊断检测装置还包括光学检测模块；所述光学检测模块包括：
    第二支架、多个光学检测单体，多个所述光学检测单体依次间隔地设置于所述第二支架上，所述光学检测单体用于对PCR腔室内的样品液体进行荧光检测；
    移动机构，所述移动机构与所述第二支架相连，所述移动机构用于驱动所述第二支架移动，以使得多个所述光学检测单体依次移动到与所述PCR腔室相对的位置。
  29. 根据权利要求28所述的体外分析诊断检测装置，其中，多个所述光学检测单体沿着第一方向依次间隔地设置于所述第二支架上，所述移动机构用于驱动所述第二支架沿着所述第一方向移动；所述第一方向为竖向方向、水平方向或与所述竖向方向设有夹角的方向。
  30. 根据权利要求29所述的体外分析诊断检测装置，其中，所述移动机构包括第三支架、第一电机、第一驱动轮、第二驱动轮、连接所述第一驱动轮与所述第二驱动轮的传动元件；所述第一电机、所述第一驱动轮与所述第二驱动轮均设于所述第三支架上；所述第一电机的转轴与所述第一驱动轮相连，用于驱动所述第一驱动轮转动；所述传动元件还与所述第二支架相连；所述第二支架滑动地设置于所述第三支架上。
  31. 根据权利要求30所述的体外分析诊断检测装置，其中，所述光学检测模块还包括第一传感器以及与第一传感器感应配合的第一触发件；所述第一传感器装设于第二支架上，所述第一触发件装设于所述第三支架上。
  32. 根据权利要求28所述的体外分析诊断检测装置，其中，多个所述光学检测单体均包括光源，多个所述光学检测单体的光源为不同波段的光源。
  33. 根据权利要求32所述的体外分析诊断检测装置，其中，所述第二支架上设有与多个所述光学检测单体一一对应设置的多个采光通道，所述光学检测单体对应地设置于所述采光通道中；所述采光通道包括第一通道与第二通道，所述第二通道与所述第一通道的中部部位垂直连通，所述光源设于所述第二通道远离于所述第一通道的端部上；所述光学检测单体还包括第一透镜、二向色镜、第二透镜、第三透镜与荧光检测器，所述第一透镜设于所述第二通道的内壁，所述二向色镜倾斜地设于所述第一通道的中部部位，所述第二透镜、所述二向色镜、所述第三透镜与所述荧光检测器依次地设于所述第一通道的内壁，所述第二透镜位于所述第一通道靠近于所述PCR腔室的一端，所述荧光检测器位于所述第一通道远离于所述PCR腔室的一端。
  34. 根据权利要求33所述的体外分析诊断检测装置，其中，所述光学检测单体还包括第一滤镜与第二滤镜，所述第一滤镜设于所述第二通道的内壁，所述第一滤镜位于所述第一透镜与所述二向色镜之间；所述第二滤镜设于所述第一通道的内壁，所述第二滤镜位于所述第二透镜与所述二向色镜之间。
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CN116769591B (zh) * 2023-08-28 2023-10-31 四川杰莱美科技有限公司 一种实时荧光定量pcr仪

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