WO2023004068A3 - Procédés, dispositifs et kits pour la purification et la lyse de particules biologiques - Google Patents

Procédés, dispositifs et kits pour la purification et la lyse de particules biologiques Download PDF

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Publication number
WO2023004068A3
WO2023004068A3 PCT/US2022/037918 US2022037918W WO2023004068A3 WO 2023004068 A3 WO2023004068 A3 WO 2023004068A3 US 2022037918 W US2022037918 W US 2022037918W WO 2023004068 A3 WO2023004068 A3 WO 2023004068A3
Authority
WO
WIPO (PCT)
Prior art keywords
biological particles
kits
methods
devices
purifying
Prior art date
Application number
PCT/US2022/037918
Other languages
English (en)
Other versions
WO2023004068A2 (fr
Inventor
Stephane Claude BOUTET
Alireza SALMANZADEH
Michael Gibbons
Original Assignee
10X Genomics, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US17/551,761 external-priority patent/US20220348901A1/en
Application filed by 10X Genomics, Inc. filed Critical 10X Genomics, Inc.
Priority to CN202280060183.9A priority Critical patent/CN117980071A/zh
Priority to EP22786133.3A priority patent/EP4373616A2/fr
Publication of WO2023004068A2 publication Critical patent/WO2023004068A2/fr
Publication of WO2023004068A3 publication Critical patent/WO2023004068A3/fr
Priority to US18/417,919 priority patent/US20240210290A1/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0854—Double walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

L'invention concerne des dispositifs, des kits et leurs procédés d'utilisation pour la lyse et/ou la purification de particules biologiques, par exemple, des noyaux. Une ou plusieurs couches thixotropiques peuvent être utilisées dans un récipient pour purifier des particules biologiques. Un dispositif muni d'éléments tranchants peut être utilisé pour lyser des particules biologiques ou le contenu de celles-ci.
PCT/US2022/037918 2021-07-21 2022-07-21 Procédés, dispositifs et kits pour la purification et la lyse de particules biologiques WO2023004068A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN202280060183.9A CN117980071A (zh) 2021-07-21 2022-07-21 用于纯化和裂解生物颗粒的方法、装置和试剂盒
EP22786133.3A EP4373616A2 (fr) 2021-07-21 2022-07-21 Procédés, dispositifs et kits pour la purification et la lyse de particules biologiques
US18/417,919 US20240210290A1 (en) 2021-07-21 2024-01-19 Methods, devices, and kits for purifying and lysing biological particles

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US202163224164P 2021-07-21 2021-07-21
US63/224,164 2021-07-21
US17/551,761 2021-12-15
US17/551,761 US20220348901A1 (en) 2020-12-15 2021-12-15 Methods, devices, and kits for purifying and lysing biological particles
US202263356751P 2022-06-29 2022-06-29
US63/356,751 2022-06-29

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US17/551,761 Continuation-In-Part US20220348901A1 (en) 2020-12-15 2021-12-15 Methods, devices, and kits for purifying and lysing biological particles

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US18/417,919 Continuation US20240210290A1 (en) 2021-07-21 2024-01-19 Methods, devices, and kits for purifying and lysing biological particles

Publications (2)

Publication Number Publication Date
WO2023004068A2 WO2023004068A2 (fr) 2023-01-26
WO2023004068A3 true WO2023004068A3 (fr) 2023-03-02

Family

ID=83598483

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2022/037918 WO2023004068A2 (fr) 2021-07-21 2022-07-21 Procédés, dispositifs et kits pour la purification et la lyse de particules biologiques

Country Status (1)

Country Link
WO (1) WO2023004068A2 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4944918A (en) * 1988-01-15 1990-07-31 Becton, Dickinson And Company Sterilization of blood component separation devices
US20100120596A1 (en) * 2007-05-23 2010-05-13 Ge Healthcare Bio-Sciences Ab Separation device
US20130225387A1 (en) * 2010-11-09 2013-08-29 Jms Co., Ltd. Separation container and separation method
WO2020019195A1 (fr) * 2018-07-25 2020-01-30 Yantai Ausbio Laboratories Co., Ltd. Gel de séparation à base de microbilles

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
EP3616781A1 (fr) 2003-04-10 2020-03-04 President and Fellows of Harvard College Formation et régulation d'espèces fluidiques
CN1842368B (zh) 2003-08-27 2014-05-28 哈佛大学 流体物种的电子控制
US20100255556A1 (en) 2007-06-29 2010-10-07 President And Fellows Of Harvard College Methods and apparatus for manipulation of fluidic species
EP3461558B1 (fr) 2009-10-27 2021-03-17 President and Fellows of Harvard College Techniques de création de gouttelettes
US11607658B2 (en) 2016-07-08 2023-03-21 President And Fellows Of Harvard College Formation of colloids or gels within droplets
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10610865B2 (en) 2017-08-22 2020-04-07 10X Genomics, Inc. Droplet forming devices and system with differential surface properties
EP3752832A1 (fr) 2018-02-12 2020-12-23 10X Genomics, Inc. Procédés de caractérisation d'analytes multiples à partir de cellules individuelles ou de populations cellulaires
WO2020139844A1 (fr) 2018-12-24 2020-07-02 10X Genomics, Inc. Dispositifs, systèmes et procédés de commande d'un écoulement de liquide
CN113747974A (zh) 2019-02-28 2021-12-03 10X基因组学有限公司 用于提高液滴形成效率的装置、系统和方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4944918A (en) * 1988-01-15 1990-07-31 Becton, Dickinson And Company Sterilization of blood component separation devices
US20100120596A1 (en) * 2007-05-23 2010-05-13 Ge Healthcare Bio-Sciences Ab Separation device
US20130225387A1 (en) * 2010-11-09 2013-08-29 Jms Co., Ltd. Separation container and separation method
WO2020019195A1 (fr) * 2018-07-25 2020-01-30 Yantai Ausbio Laboratories Co., Ltd. Gel de séparation à base de microbilles

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FORD T ET AL: "Iodixanol: A Nonionic Iso-osmotic Centrifugation Medium for the Formation of Self-Generated Gradients", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, AMSTERDAM, NL, vol. 220, no. 2, 1 August 1994 (1994-08-01), pages 360 - 366, XP024763204, ISSN: 0003-2697, [retrieved on 19940801], DOI: 10.1006/ABIO.1994.1350 *
SUNG-SIK YUN ET AL: "Handheld mechanical cell lysis chip with ultra-sharp silicon nano-blads arrays for rapid intracellular protein extraction", LAB CHIP,, vol. 10, 16 March 2010 (2010-03-16), pages 1442 - 1446, XP002766650 *

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