WO2022247833A3 - Composition, kit, method, and use thereof for detecting sars-cov-2 mutation sites - Google Patents

Composition, kit, method, and use thereof for detecting sars-cov-2 mutation sites Download PDF

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Publication number
WO2022247833A3
WO2022247833A3 PCT/CN2022/094749 CN2022094749W WO2022247833A3 WO 2022247833 A3 WO2022247833 A3 WO 2022247833A3 CN 2022094749 W CN2022094749 W CN 2022094749W WO 2022247833 A3 WO2022247833 A3 WO 2022247833A3
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WO
WIPO (PCT)
Prior art keywords
composition
cov
detection
sars
mutation sites
Prior art date
Application number
PCT/CN2022/094749
Other languages
French (fr)
Other versions
WO2022247833A2 (en
Inventor
Lizhong Dai
Deyong TAN
Jia Liu
Zhongping DENG
Qingzhi SUN
Kang Wu
Xing Cheng
Xiaomei REN
Tangjie GAO
Xinwu GUO
Original Assignee
Sansure Biotech Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CN202110592489.6A external-priority patent/CN115404237B/en
Priority claimed from CN202111503790.1A external-priority patent/CN116254367A/en
Priority claimed from CN202111667275.7A external-priority patent/CN114561490B/en
Application filed by Sansure Biotech Inc. filed Critical Sansure Biotech Inc.
Priority to EP22735762.1A priority Critical patent/EP4232608A2/en
Publication of WO2022247833A2 publication Critical patent/WO2022247833A2/en
Publication of WO2022247833A3 publication Critical patent/WO2022247833A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to the field of molecular biology detection, specifically to detection of SARS-CoV-2, and more specifically to detection of major mutation sites of SARS-CoV-2 variants. Further provided are a kit comprising a composition, a use of the composition, and a method for detecting and genotyping of SARS-CoV-2 variants. By using the composition in the present invention, major mutations of the novel coronavirus variants can be identified, and preliminary screening and confirmation of variant types are performed by site-combination detection, so that different mutation sites can be treated differently, thereby leading to more effective treatment and prevention. The composition of the present invention, in combination with a fluorescent probe method, can be used to detect multiple targets simultaneously, with advantages of a low cost, a high throughput, a simple operation and a short usage time.
PCT/CN2022/094749 2021-05-28 2022-05-24 Composition, kit, method, and use thereof for detecting sars-cov-2 mutation sites WO2022247833A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP22735762.1A EP4232608A2 (en) 2021-05-28 2022-05-24 Composition, kit, method, and use thereof for detecting sars-cov-2 mutation sites

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
CN202110592489.6 2021-05-28
CN202110592489.6A CN115404237B (en) 2021-05-28 2021-05-28 Combination product, kit, use and method for detecting mutant SARS-CoV-2 virus
CN202111500433.X 2021-12-09
CN202111500433 2021-12-09
CN202111503790.1 2021-12-10
CN202111503790.1A CN116254367A (en) 2021-12-10 2021-12-10 Compositions, kits, methods and uses for detecting SARS-CoV-2 mutation
CN202111667275.7A CN114561490B (en) 2021-12-09 2021-12-31 Composition, kit and method for detecting SARS-CoV-2 mutation site and application thereof
CN202111667275.7 2021-12-31

Publications (2)

Publication Number Publication Date
WO2022247833A2 WO2022247833A2 (en) 2022-12-01
WO2022247833A3 true WO2022247833A3 (en) 2023-01-19

Family

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PCT/CN2022/094749 WO2022247833A2 (en) 2021-05-28 2022-05-24 Composition, kit, method, and use thereof for detecting sars-cov-2 mutation sites

Country Status (2)

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EP (1) EP4232608A2 (en)
WO (1) WO2022247833A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112725537A (en) * 2020-03-12 2021-04-30 宁波海尔施基因科技有限公司 Multiplex real-time fluorescent quantitative PCR (polymerase chain reaction) kit and method for detecting 2019 novel coronavirus and primer probe composition
WO2021176099A1 (en) * 2020-03-05 2021-09-10 Institut Pasteur Methods and reagents for the specific and sensitive detection of sars-cov-2
CN114107572A (en) * 2022-01-26 2022-03-01 潮州凯普生物化学有限公司 Primer probe set for detecting different new coronavirus mutant strains based on multiplex PCR technology, detection kit and application of detection kit

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021176099A1 (en) * 2020-03-05 2021-09-10 Institut Pasteur Methods and reagents for the specific and sensitive detection of sars-cov-2
CN112725537A (en) * 2020-03-12 2021-04-30 宁波海尔施基因科技有限公司 Multiplex real-time fluorescent quantitative PCR (polymerase chain reaction) kit and method for detecting 2019 novel coronavirus and primer probe composition
CN114107572A (en) * 2022-01-26 2022-03-01 潮州凯普生物化学有限公司 Primer probe set for detecting different new coronavirus mutant strains based on multiplex PCR technology, detection kit and application of detection kit

Non-Patent Citations (11)

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EP4232608A2 (en) 2023-08-30

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