WO2022229984A1 - Composition naturelle d'extraits végétaux pour lutter contre des pathogènes parasites et des nématodes végétaux - Google Patents

Composition naturelle d'extraits végétaux pour lutter contre des pathogènes parasites et des nématodes végétaux Download PDF

Info

Publication number
WO2022229984A1
WO2022229984A1 PCT/IN2022/050402 IN2022050402W WO2022229984A1 WO 2022229984 A1 WO2022229984 A1 WO 2022229984A1 IN 2022050402 W IN2022050402 W IN 2022050402W WO 2022229984 A1 WO2022229984 A1 WO 2022229984A1
Authority
WO
WIPO (PCT)
Prior art keywords
composition
oil
plant
extract
natural
Prior art date
Application number
PCT/IN2022/050402
Other languages
English (en)
Inventor
Mrs Sunita AHIRE
Original Assignee
Ahire Mrs Sunita
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ahire Mrs Sunita filed Critical Ahire Mrs Sunita
Publication of WO2022229984A1 publication Critical patent/WO2022229984A1/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
    • A01N31/16—Oxygen or sulfur directly attached to an aromatic ring system with two or more oxygen or sulfur atoms directly attached to the same aromatic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P5/00—Nematocides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00—Arthropodicides
    • A01P7/02—Acaricides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00—Arthropodicides
    • A01P7/04—Insecticides

Definitions

  • the present invention relates generally to the pesticide composition and more particularly to a synergistic plant-based composition mainly used to control insects, acarus, aphids, termites, nematodes, viruses and bacteria in agriculture field and domestic and civil disinfestation.
  • the present invention specifically relates to a composition which based on natural extracts.
  • compositions have been in use at great extent in the pesticides, which are being used to control the insects, termites, nematodes and so forth. Further several natural compositions are also in use to achieve the same goal, which show less or no toxicity to the consumers who consume it, though they can have severe impact on the environment as they are not easily eliminated.
  • the compositions can specifically target the insects, termites, nematodes and so forth, but can pose as a toxic to non-target species.
  • Plant parasitic nematodes cause significant damage to a wide variety of crops, resulting in global crop yield losses estimated to range from 5% to 12% annually. Root damage by nematodes is very common and leads to stunted plants, which have smaller root systems, show symptoms of mineral deficiencies in their leaves and wilt easily. Damage by nematodes also predisposes plants to infection by a wide variety of plant pathogenic fungi and bacteria.
  • the present invention provides a safe and effective means to replace or lessen the use of chemical pesticides. It is also unique in providing a solution that both inhibits nematode penetration into the plant root and then prevents maturation of those nematodes that manage to overcome this initial barrier.
  • Plants are constantly challenged by a wide variety of pathogenic organisms including viruses, bacteria, fungi, and nematodes. Attempts have been made to control plant disease by means of disinfections, replacement of the soil, various cultural practices, and control by chemicals. Some plants suffer from detrimental soil-spread diseases, which have not been possible to control owing to restrictions of use of chemical control agents and hazard periods due to possible residues or lack of sufficiently effective products.
  • the control of fungi is important since fungal growth on plants or on parts of plants inhibits production of foliage, fruit or seed, and the overall quality of a cultivated crop. About twenty-five (25) percent of all fungal diseases in agricultural and horticulture are caused by powdery mildew phytopathogens.
  • WIPO patent application number 2015043986A1 by Chemia S.P.A. discloses a pesticide based on natural extracts including Thymol, an alkaloid Matrine, Carvacrol and at least one molecule chosen from Ossimatrine, Sophocarpine, N- oxysophocarpine, Cytisine, Aloperine, Carangine and Toosedanin.
  • Said pesticide is particularly suitable for use in agriculture, gardening, hobbies, veterinary, for wood protection, domestic and civil environment, open or closed public places against theharmful effects of insects, mites, aphids, nematodes and virus diseases of plants.
  • US patent application 8501247B2 by Tyratech Inc, Mondelez Global LLC discloses pest control using natural pest control agent blends for controlling a target pest, wherein the composition includes at least two active ingredients selected from the group consisting of thymyl acetate, linalyl acetate, amyl butyrate, anise star oil, black seed oil, p-cymene, geraniol, isopropyl myristate, d-limonene, linalool, lilac flower oil, methyl salicylate, alpha-pinene, piperonal, piperonyl alcohol, tetrahydrolinalool, thyme oil white, thyme oil red, thymol, vanillin, and winter- green oil, wherein the composition causes synergistic control of the target pest.
  • the composition includes at least two active ingredients selected from the group consisting of thymyl acetate, linalyl acetate, amyl butyrate, anis
  • US patent 20060194698A by University of Tennessee Research Foundation discloses the use of herbs as a delivery system for bioactive phytochemicals for controlling weeds, plant pests, or plant pathogens comprising the application of a bioactive herbage (plant material) composition to soil as a soil amendment or as top dressing for potted plants.
  • the method of controlling weeds, plant pests, or plant pathogens comprises the application of a bioactive herbage (plant material) composition to soil as a soil amendment or as top dressing for potted plants in amounts sufficient to control weeds, plant pests, or plant pathogens, wherein said bioactive herbage is obtained from: (a) Monarda spp.;(b) Chamaemelum spp.; (c) Matricaria spp.; (d) Chenopodium spp.; or (e) various combinations of thereof.
  • Herbage can be dried or wet and other sources of bioactive herbage are also suitable for use in the subject invention is provided.
  • WIPO patent application 2006097700A1 by Plant Impact Pic discloses plant growth enhancement and method thereof wherein said method comprising applying to a plant or to the environment thereof, an agricultural composition comprising (i) one or more essential oils, or active components thereof, or mixtures of these, (ii) a plant oil and (iii) an emulsifier.
  • Such compositions enhance plant growth, in particular by enhancing nutrient uptake from the soil or the environment.
  • Compositions for use in the method are also described and claimed.
  • Indian patent application number 245/MUM/2004 by North Maharashtra University discloses A process for preparing a botanical pesticide from seeds of Annona Squamosa, characterized in that (i) defatting seed powder of A. squamosa with defatting solvent(s); (ii) treating the residue cake obtained in step (i) with a polar solvent; (iii) filtering the extract of step (ii); (iv) concentrating the filtered extract of step (iii); (v) partitioning the concentrated extract of step (iv) with a suitable extracting solvent; (vi) adding suitable dehydrating agent in extract of step (v); (vii) completely drying the extract of step (vi); (viii) dissolving the residue of step (vii) in a suitable extracting solvent; (ix) further defatting extract of step (viii) with defatting solvent; (x) drying completely/partially the extract of step (ix); (xi) dissolving the residue of step (x) in a suitable polar solvent and (xi
  • the disinfecting compositions according to the invention should exhibit the lowest possible toxicity, be ecologically compatible, i. show no accumulation in groundwater and food chain. Despite these properties, the compositions according to the invention should nevertheless have strong and long- lasting disinfecting effects and thus offer reliable protection against microorganisms
  • the primary object of the present invention is to provide a composition to control plant pathogenic fungi and parasitic nematodes.
  • Another object of the present invention is to provide a composition of plant extracts which is organic alternative to the chemical-based pesticides.
  • Yet another object of the present invention is to provide a plant based composition
  • a plant based composition comprising of extract of Thymus Vulgaris containing Monoterpenoid phenol derivatives of p-cymene isomeric with carvacrol polyphenols as an anti-fungal agent, Extract of Annona Squamosa containing acetogenins from class of polyketide containing 32 or 34 carbon chain having oxygenated functional group including Hydroxyls, Ketones, Expoxides, Tetrahydrofuran and Tetrahydropyrans as insecticidal agent and 5-Chloro-2-(4-chlorophenoxy) phenol as anti bacterial, anti-viral agent.
  • the present invention discloses a natural synergistic composition of plant extracts which acts anti-fungal, anti-bacterial and insecticidal composition.
  • the said synergistic composition comprises of plant-based extracts wherein the various forms of the said composition comprising of Organic or aqueous extract of Thymus vulgaris as anti-fungal component, Organic or aqueous extract of Annona Squamosa as insecticidal component, 5-Chloro-2-(4- chlorophenoxy) phenol asanti-bacterial component.
  • Surfactants preferably selected from the group of Naturally Ethoxylated/Propoxylated or Ethoxylated-Propoxylated Natural Vegetable oil including but not limited to castor oil, Palm Oil, Coconut Oil, Soybean oil as natural surfactant.
  • any other surfactant which is having HLB (Hydrophile Lypophile Balance) of 9 to 20 can be used in the formulation described in the present invention.
  • Diluents preferably selected from the group of but not limited to Natural oil and its derivatives from the sources like soyabean, cotton or palm, and 2-ethyl hexyl lactate/dimethyl sulfoxide/water/N, N Dimethyl decanamide and water.
  • the said plant-based composition is highly effective, natural and low toxic composition, which can be used to control plant pathogenic fungi, plant nematodes, viruses and bacteria.
  • aspects of the present disclosure relates to a composition based on the natural extracts to eliminate and control the plant nematodes and to more particularly to a synergistic plant- based composition mainly used to control insects, acarus, aphids, termites, nematodes, Bacteria and viruses in agriculture field and domestic and civildisinfestation.
  • the present invention discloses a natural synergistic comprises of plant-based extracts wherein the various forms of the said composition comprising of Organic or aqueous extract of Thymus vulgaris as anti-fungal component, Organic or aqueous extract of Annona Squamosa as insecticidal component, 5-Chloro-2-(4- chlorophenoxy) phenol as anti-bacterial component.
  • the synergistic composition comprises of plant-based extracts wherein the various forms of the said composition comprising of Organic or aqueous extract of Thymus vulgaris as anti-fungal component, which acts against the fungal infections seen in the plants.
  • Thymus Vulgaris has antibacterial, antifungal and antivirus properties.
  • the antifungal potential of Thymus Vulgaris it can be used in agriculture as a fungicide.
  • the main advantage of using Thymus vulgaris as a pesticide is that it has very low toxicity and can rapidly decompose after it is applied on the plants thus safe for any other handlers or consumers who come in contact with the plant.
  • Thymus Vulgaris is a widely available Lamiaceae which is a native of meridional Europe, with its essential oil mainly composed of two phenolic monoterpenes, carvacrol and/or thymol, which are highly responsible for is antifungal activity.
  • Fungi constitute the largest number of plant pathogens and are responsible for a range of serious plant diseases which damage plants by killing cells and/or causing plant stress.
  • the organic or aqueous extract of Thymus Vulgaris can help in combating the fungal infections seen on various parts of the plants.
  • Some of the fungi are which are responsible for fungal diseases include downy mildews; powdery mildews; white blister, etc., There are several other fungi which causes diseases in the plants.
  • the vegetable plants which are grown also get infected with some fungal diseases including anthracnose; botrytis rots; downy mildews; fusarium rots; powdery mildews; rhizoctonia rots; sclerotinia rots; sclerotium rots.
  • the extract of Thymus Vulgaris with the properties can be used to combat the fungal disease.
  • the extract of Thymus Vulgaris can actively act against the fungal infections which are spread on the plants.
  • Grape plant the downy mildews; powdery mildews are the pathogens which affect the grape plant.
  • the most common fungal diseases that infect tomato plants include Anthracnose fruit rot; wilts, early blight, Septoria leaf spot, Fate blight, and Buckeye rot all which produce distinct symptoms.
  • the fungal infections such as such as grey leaf spot, northern com leaf blight, tar spot and common and southern rust, and so forth. With the help of the said synergistic composition, the diseases spreadon the grape plant can be treated.
  • the present invention discloses a synergistic composition which comprises of organic or aqueous extract of Annona Squamosa which acts as an insecticidal component.
  • the Annona Squamosa extract or the custard apple (Common Name of Annona Squamosa) is a potential medicinal plant from the family Annonaceae, with diversified medicinal and pesticidal use, which can be cultivated in all tropical and subtropical countries including India.
  • Annona Squamosa extract is used in the pesticide composition as an insecticidal agent which contains squamocin as an active ingredient.
  • the Annona Squamosa seeds also contain acetogenins, which inhibits the development of the microbes on the plants and acts as insect control for plant sources.
  • the extract of the Annona Squamosa is non-toxic to the animals but have pesticidal and / or insecticidal properties which can combat any insecticidal infections.
  • the use of Annona Squamosa can be used on a wide variety of plants which can include pulses, millets, fibre crops, sugar crops, plantation crops, vegetables, fruits, spices, flowers, medicinal crops and so forth.
  • the present invention comprises of a component 5-Chloro-2-(4-chlorophenoxy) phenol which acts as an anti-bacterial component.
  • 5-Chloro-2-(4-chlorophenoxy) phenol is a polychloro phenoxy phenol which is aromatic ether that is phenol which is substituted at C- 5 by a chloro group and at C-2 by a 2, 4-dichlorophenoxy group. It works as an antibacterial agent
  • 5-Chloro-2-(4-chlorophenoxy) phenol acts as biocide specifically at lower concentrations (100 ppm- 500 ppm), which attacks multiple cytoplasmic and membrane of the bacteria, to eliminate the bacterial infection.
  • 5-Chloro- 2-(4-chlorophenoxy) phenol at lower concentrations binds to bacterial enoyl-acyl carrier protein reductase enzyme which destabilizes it to combat the bacterial infections.
  • 5-Chloro-2-(4-chlorophenoxy) phenol or 5-Chloro-2-(4-chlorophenoxy) phenol which is the antibacterial ingredient which acts to slow or stop the growth of bacteria, fungi, and mildew.
  • 5-Chloro-2-(4-chlorophenoxy) phenol which can be directly be applied on the plants to prevent microbial growth.
  • the present invention comprises of another component Ethoxylated/Propoxylated or Ethoxylated-Propoxylated Natural Vegetable oil including but not limited to castor oil, Palm Oil, Coconut Oil, Soybean oil as natural surfactant.
  • the said Ethoxylated/Propoxylated or Ethoxylated-Propoxylated Natural Vegetable oil molecules spontaneously bond with each other to form sealed bubbles as they have lower the surface tension.
  • These surfactants may act as emulsifiers wherein the said surfactant is used in the synergistic composition as a part of formulation which creates an oil/water emulsion when the concentrate is added to water.
  • the formulation components of the said composition includes Ethoxylated/Propoxylated or Ethoxylated- Propoxylated Natural Vegetable oil acts as a natural surfactant, can spontaneously bond with the all the components of the said composition. Additionally, any other surfactant which is having HLB (Hydrophile Lypophile Balance) of 9 to 20 can be used in the formulation described in the present invention.
  • HLB Hydrophile Balance
  • the present invention comprises of another component which is natural oil and its derivatives from the sources like soyabean, cotton or palm.
  • the liquid formulations which are used in the said synergistic composition where the active ingredients are dissolved in the said natural oil and the surfactant is used in the said formulation so that it can be mixed with water for spraying.
  • the natural oils and its derivatives like soya oil, cotton oil or palm oil which acts as stabilizers or solvents, which enables to preserve the desirable properties of the active ingredients of the said composition until it is prepared for desired application and applied on the plants.
  • natural oil and its derivatives from the sources like soybean, cotton or palm, and 2-ethyl hexyl lactate/dimethyl sulfoxide/water/N, N Dimethyl decanamide and water can be used as a diluent is basically used to dilute the said synergistic composition.
  • the said composition comprises of a diluent and an emulsifier which prevents the said composition from separating, and the diluent dilutes the pesticide to make it safer, more effective, and easier to measure, mix, apply, and handle.
  • the said synergistic composition can be applied to the plants using to any type of common application equipment including but not limited to basic sprayer or a hydraulic sprayer, mist blowers and so forth.
  • the said composition has high potential as an alternative to synthetic pesticides which are exiting in the market and their associated negative effects.
  • the synergistic composition as disclosed in the present invention can be prepared using various formulations which can include emulsifiable concentrate, micro- emulsion, oil dispersion, suspension concentrate, wettable powder, water dispersible granules and so forth.
  • Table 1 The formulation used to develop the emulsifiable concentrate of the synergistic composition
  • the process of formulating the emulsifiable concentrate of the synergistic composition is as follows: In laboratory mixer (DISPERMAT-R30 supplied by VMA-GETZMANN GMBH), the required quantity of Solvent/Diluent is weighed and added and mixed with continuous stirring; organic extract of Thymus Vulgaris is added in the mixer and stirred well until it dissolves, and while continuous stirring, the organic extract of Annona Squamosa is added and mixed until it forms clear solution. If required, the said mixture is heated to 40 to 45 degree Celsius.
  • the ethoxylated derivatives of castor oil (Emulsogen EL-40 from Clariant, Agnique CSO 36 from BASF, Alkamuls 40 - Solvey) is added and mixed until it forms a clear solution.
  • the desired amount of Calcium alkyl benzene sulfonate (Agnique ABS-60-CE-H BASF, Ninate 70 B-Stepan Chemical Co, Rhodacal 60BR-Solvey) is weighed and added to the mixture, mixing it continuously by stirringuntil it is completely dissolved.
  • Table 2 The formulation is tested for physico-chemical properties as per below specifications
  • the formulation used to develop the Micro emulsion formulation includes:
  • the process of the formulation of the micro emulsion formulation includes weighing desired amount of water (aqueous phase) in laboratory mixer (DISPERMAT-R30 supplied by VMA-GETZMANN GMBH) and adding Propylene Glycol (Kollisolv PG-BASF) (organic phase) and Defoamer (Agnique DFM-lll-S) and mix until homogeneous solution with constant stirring.
  • aqueous phase aqueous phase
  • DISPERMAT-R30 supplied by VMA-GETZMANN GMBH
  • Propylene Glycol Kersolv PG-BASF
  • organic phase organic phase
  • Defoamer Agnique DFM-lll-S
  • N, N Dimethyl decanamide (Agnique AMD-10-BASF) solvent in a separate beaker and stirring continuously to mix well, and in same beaker weigh desired amount of organic extract of Thymus vulgaris and mix till dissolves, and in same beaker weigh and add desired amount of organic extract of Annona Squamosa followed by 5-Chloro-2- (4-Chlorophenoxy) phenol and stir until they are completely dissolved.
  • N, N Dimethyl decanamide Agnique AMD-10-BASF
  • the product is then analysed for active matter and then proceeded for further addition of Soybean oil, followed by calcium alkyl benzene sulfonate(Rhodacal 65-BR-Solvey), and castor oil ethoxylate (Agnique CSO-36-BASF) and stir well until it completely dissolves. Then slowly add aqueous phase in to organic phase with stirring at high speed of 1500 to 2000 rpm to form white milky suspension of particle size less than 5 microns. To this suspension, add Xanthan gum 2% solution (Agropol-23 -Solvey) to modify viscosity.
  • Soybean oil followed by calcium alkyl benzene sulfonate(Rhodacal 65-BR-Solvey), and castor oil ethoxylate (Agnique CSO-36-BASF) and stir well until it completely dissolves. Then slowly add aqueous phase in to organic phase with stirring at high speed of 1500 to 2000 rpm
  • the formulation for oil dispersion composition is:
  • the process of the formulation of oil-dispersion composition includes: In laboratory mixer (DISPERMAT-R30 supplied by VMA-GETZMANN GMBH) weigh desired amount of Methylated soybean oil and desired amount of Alcohol Ehtloylate (Lutensol TO 5-BASF) and calcium alkyl Benzene sulfonate (Rhodacal 6 BR- Solvey) and mix well to form dispersion along with constant stirring. Keeping the constant stirring, add Gerenol Odessy 5 supplied by solver and stir well to form homogeneous mix.
  • DISPERMAT-R30 supplied by VMA-GETZMANN GMBH
  • the formulation for suspension concentrates of the composition is:
  • the process of preparation of the said formulation includes: In a separate beaker equipped with homogenization (DISPERMAT-R30 supplied by VMA GETZMANN GMBH) facility weigh desired amount and DM water followed by Soprophor FLK, Agnique CP-72 L- Supplied by BASF and Pluronic PE 10500- Supplied by BASF with slow stirring. Weigh and add propylene glycol and Agnique DFM-111 -S-Supplied by BASF with constant stirring to form a solution. Increase the speed to 2000 rpm and slowly add kaolin clay to form a suspension.
  • homogenization DISPERMAT-R30 supplied by VMA GETZMANN GMBH
  • the product specification of the said specification is:
  • the product specification at Stability Studies at 54 Deg C discloses the following:
  • the wettable powder formulation of the said composition includes:
  • the process of preparation of the said composition includes: In a ribbon blender (Sanitary lab model 1 cu ft- Supplied by ROSS USA) which is charged with Tamol DN supplied BASF, Tamol FBPP Supplied by BASF and Supragil WP Supplied by Solvey were mixed together to form uniform mass. This mass is then fed to dry grinding mill air jet mill (Supplied by Promas machinery India), where the mixture is ground to the particle size of D-90- 20 microns and again fed to post blender and mixed for 1 hr to finish the dry blending part of the formulation.
  • the formulation for water dispersible granules is:
  • the process of formulation of the said product includes: The ribbon blender (Sanitary lab model 1 cu ft- Supplied by ROSS USA) is charged with Tamol DN supplied BASF, Tamol FBPP Supplied by BASF and Supragil WP Supplied by Solvey were mixed together to form uniform mass. This mass is then fed to dry grinding mill air jet mill (Supplied by Promas machinery India) where the mixture is ground to the particle size of D-90- 20 microns and again fed to post blender and mixed for 1 hr to finish the dry blending part of the formulation.
  • the present invention further discloses the bio-efficacy of the said synergistic composition showing the nematicide activity of various formulations.
  • Nirmal were directly sown and four-week- old seedlings, raised in autoclaved soil, of tomato (Lycopers'con lycopersicum (L) Karsten).
  • cv. Pusha Sadabahar. Snowball were transplanted. Necessary cultivation practices were observed. Nematode populations of each treatment were determined with the help of Oostenbrink's elutriator and Baermann funnels (Southey, 1970), both prior to treatment as well as at the termination of the experiment (three months).
  • Hop Hoplolaimus indicus
  • Hel Helicotylenchus indicus
  • T yl Tylenchus filifonnis
  • Mel Meloidogyne incognita larvae
  • Tr i Trichodorous mirzai Test 2: Minimum Inhibitory Concentration
  • the formulations prepared as per the examples are tested for minimum inhibitory concentration (MIC) for gram-positive and gram-negative bacteria.
  • MIC minimum inhibitory concentration
  • Tl The treatment with Tl, T2, T3, T4, T5, T6 and TO serve as control.
  • the dose 2 ml/liter and 3 ml/liter were used to evaluate the results, whereas TO control plot were treated with plain water.
  • the observations were made after 30,45 and 60 days respectively.
  • the results were showed that all the fungicide treatments were effective to reduce substantially the fungicide index, however the Tl (EC formulation), T2(OD formulation) and T3 (ME formulation) have showed better control over the other formulation and untreated control # 2 and 3 ml/liter of formulation, 3 ml / liter were substantially effective concentration to control check of fungicide index.
  • the Percent Disease Index (PDI) was calculated using the formula below.
  • PKI Percent Disease Index
  • Extract of Thymus vulgaris as anti-fungal component 8.0% w/w Organic/aq. Extract of Annona Squamosa as insecticidal component: 10% w/w 5-Chloro-2-(4-chlorophenoxy) phenol as anti-bacterial component: 10% w/w Ethoxylated castor oil/Ethoxylated soybean oil another natural surfactant: 25% w/w Natural oil and its derivatives (Soya. Cotton/palm): 25% w/w 2, ethyl hexyl lactate/dimethyl sulfoxide/water/N, N Dimethyl decanamide: up to 100% w/w of total volume.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Insects & Arthropods (AREA)
  • Biotechnology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

La présente invention concerne une composition synergique naturelle d'extraits végétaux qui agit comme une composition antifongique, antibactérienne et insecticide. Ladite composition synergique comprend des extraits végétaux, les diverses formes de ladite composition comprenant un extrait organique ou aqueux de Thymus vulgaris en tant que composant antifongique, un extrait organique ou aqueux d'Annona Squamosa en tant que composant insecticide, du 5-chloro-2-(4-chlorophénoxy) phénol en tant que composé anti-bactérien.
PCT/IN2022/050402 2021-04-29 2022-04-28 Composition naturelle d'extraits végétaux pour lutter contre des pathogènes parasites et des nématodes végétaux WO2022229984A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN202121019585 2021-04-29
IN202121019585 2021-04-29

Publications (1)

Publication Number Publication Date
WO2022229984A1 true WO2022229984A1 (fr) 2022-11-03

Family

ID=83847874

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2022/050402 WO2022229984A1 (fr) 2021-04-29 2022-04-28 Composition naturelle d'extraits végétaux pour lutter contre des pathogènes parasites et des nématodes végétaux

Country Status (1)

Country Link
WO (1) WO2022229984A1 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101808993B (zh) * 2007-09-26 2014-06-25 朗盛德国有限责任公司 杀生物的混合物
EP2763541A1 (fr) * 2011-10-04 2014-08-13 0903608 B.C. Ltd. Formulations de lutte contre les nuisibles et leurs procédés de fabrication et d'utilisation
IN2011KO01068A (fr) * 2011-08-16 2014-10-17 Raju Kumar Mahato

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101808993B (zh) * 2007-09-26 2014-06-25 朗盛德国有限责任公司 杀生物的混合物
IN2011KO01068A (fr) * 2011-08-16 2014-10-17 Raju Kumar Mahato
EP2763541A1 (fr) * 2011-10-04 2014-08-13 0903608 B.C. Ltd. Formulations de lutte contre les nuisibles et leurs procédés de fabrication et d'utilisation

Similar Documents

Publication Publication Date Title
Halder et al. Compatibility of neem oil and different entomopathogens for the management of major vegetable sucking pests
CA3108585C (fr) Nano-emulsion antimicrobienne
US7208519B2 (en) Pesticidal compounds and compositions
US10912294B2 (en) Composition for use as pesticide, fertilizer, or both, and uses thereof
US20070237837A1 (en) Biocompatible tea tree oil compositions
EP1541023B1 (fr) Combinaison ayant une activité biocide pour des applications agricoles
Ayoub et al. In field control of Botrytis cinerea by synergistic action of a fungicide and organic sanitizer
Gupta Efficacy of neem in combination with cow urine against mustard aphid and its effect on coccinellid predators
Agbo et al. Bio-pesticidal properties of Neem (Azadirachta indica)
Dar et al. Biopesticides–Its Prospects and Limitations: An Overview
Singh et al. Bioefficacy, environmental safety and synergistic impacts of biorational formulations against whitefly, leafhopper and blister beetle in organic okra ecosystem
Joseph et al. Safety of neem products to tetragnathid spiders in rice ecosystem
US20150072030A1 (en) Biocompatible tea tree oil compositions
WO2022229984A1 (fr) Composition naturelle d'extraits végétaux pour lutter contre des pathogènes parasites et des nématodes végétaux
CA2761623A1 (fr) Melange pour la lutte antiparasitaire biologique et methodes pour l'utiliser
CN105475346B (zh) 一种杀虫组合物
CN113164885B (zh) 抗微生物纳米乳剂
Gangwar et al. Problems causing due to chemical pesticides and its effect on environment
US20020031538A1 (en) Light activateable natural pesticide formulations
Nguyễn et al. Synergism of Tephrosia vogelii and Piper aduncum Based Nanoemulation Against Spodoptera frugiperda
Abdullah et al. Biopesticides: A Green Substitute to Chemical Pesticide
Angaye et al. Plant-Derived Pesticides (PDPs) Versus Synthetic Pesticides: An Eco-Friendly Approach To Biodiversity Conservation
BIRADAR EFFICACY OF NEEM BASED INSECTICIDES FOR THE MANAGEMENT OF LINSEED BUD FL GEMENT OF LINSEED BUD FL GEMENT OF LINSEED BUD FLY (Dasyneura lini Dasyneura lini Dasyneura lini Barnes)
CN106689203B (zh) 一种作物病虫害防除组合物
TWI378772B (en) The formulation of microemulsion of plant protectant, spreader, nematicide and agricultural chemical on control of plant root-knot nematode diseases

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 22795164

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 22795164

Country of ref document: EP

Kind code of ref document: A1