WO2022217479A1

WO2022217479A1 - Sample liquid protection device, processing method therefor, sample liquid containing device and sample liquid loading method

Info

Publication number: WO2022217479A1
Application number: PCT/CN2021/087121
Authority: WO
Inventors: 高岿
Original assignee: 嘉兴奥振生物科技有限公司
Priority date: 2021-04-14
Filing date: 2021-04-14
Publication date: 2022-10-20
Also published as: CN117015595A

Abstract

A sample liquid protection device, a processing method therefor, a sample liquid containing device and a sample liquid loading method, the sample liquid protection device being used for cooperating with an opening of a sample tube (300), the sample liquid protection device comprising: a sample tube cover (100); and a retention cover (200), which is a cylindrical structure, and both ends thereof in the axial direction are provided with a connecting structure, which are respectively used for detachable sealed cooperation with the sample tube (300) and the sample tube cover (100). The interior of the retention cover (200) comprises a plurality of pierceable sealing films (210) which are arranged at intervals, and a cleaning scrape blade (220) is provided between two sealing films (210). The device reduces the risk of sample contamination and increases automation.

Description

Sample liquid protection device and processing method thereof, as well as sample liquid containing device and sample liquid loading method

technical field

The present application relates to the medical field, and in particular, to a sample liquid protection device and a processing method thereof, as well as a sample liquid containing device and a sample liquid loading method.

Background technique

The existing high-throughput diagnostic system has a high risk of cross-contamination during sample processing, and there is a high risk of cross-contamination during sample extraction and transfer. For example, residual liquid on the outer wall of the tip, Residual liquid in the tip caused by viscous samples, dragging in the tip caused by problematic samples, etc. All of the above will increase the risk of contamination of the fully automated system, so the uncap and cap of the sample tube has become the bottleneck of high-throughput detection.

technical problem

The present application provides a sample liquid protection device, which solves the problem of easy contamination during sample processing in high-throughput diagnostic systems in the prior art.

technical solutions

The sample liquid protection device of the present application is used to cooperate with the nozzle of the sample tube, and the sample liquid protection device includes:

Sample tube cover;

The retention cover is a cylindrical structure, and both ends in the axial direction are provided with connecting structures, which are respectively used for detachable sealing cooperation with the sample tube and the sample tube cover, and the interior of the retention cover includes spaced arrangements The multi-channel pierceable sealing film is provided with a cleaning blade between the two sealing films.

Several optional methods are also provided below, which are not intended to be additional limitations on the above-mentioned overall solution, but are merely further additions or optimizations. On the premise of no technical or logical contradiction, each optional method can be independently implemented for the above-mentioned overall solution. The combination can also be a combination between multiple optional ways.

Optionally, the connection structure is a thread or a snap-fit structure.

Optionally, the sample tube cover is a cylindrical structure with one end closed, and a second sealing gasket is arranged inside the closed end. In the use state, the second sealing gasket is clamped and fixed between the retention cover and the retaining cover. between the sample tube caps.

Optionally, the sample tube cover is provided on the outer periphery of the retention cover.

Optionally, the outer wall of the sample tube cover is provided with anti-skid patterns.

Optionally, the sealing film has two layers, which are a top aluminum foil layer and a bottom aluminum foil layer respectively, and the cleaning blades are arranged layer by layer, and there are two or more layers;

All cleaning blades are distributed between the top foil layer and the bottom foil layer.

Optionally, the side of the retention cover facing the sample tube is located outside the sample tube, and the side facing the sample tube cover is located inside the sample tube cover.

Optionally, the retention cover includes a first connection sleeve sleeved on the outside of the sample tube along its own axial direction, and a second connection sleeve inserted into the sample tube cover;

The connection structure includes an inner thread arranged on the first connection sleeve and an outer thread arranged in the second connection sleeve;

The top aluminum foil layer is located at one end of the second connection sleeve away from the first connection sleeve, and the bottom aluminum foil layer is located between the first connection sleeve and the second connection sleeve.

Optionally, the pitch of the inner thread is smaller than the pitch of the outer thread.

Optionally, the inner thread has an engaging structure, and the disengagement resistance of the engaging structure is greater than the unscrewing resistance of the outer thread.

Optionally, the retention cap has opposite top and bottom ends, wherein the side facing the sample tube cap is the top end, the interior of the retention cap and the connectable area of the sample tube form a sampling channel, the The inner diameter of the sampling channel extends equally or gradually expands from the top end to the bottom end.

Optionally, the inner diameter of the sampling channel is gradually enlarged from the top end to the bottom end through a stepped structure and/or a gradual structure.

Optionally, an installation step is provided inside the retention cover, and the installation step is located at the joint part of the first connection sleeve and the second connection sleeve;

The installation step is stacked with a first sealing gasket on the side facing the bottom end of the retention cap, and in a use state, the first sealing gasket is clamped and fixed between the nozzle of the sample tube and the installation step.

Optionally, along the axial direction of the retaining cover, the second connecting sleeve is a multi-segment structure, and each layer of cleaning blades is clamped and fixed between two adjacent segments.

Optionally, the cleaning blade has two layers, and the second connecting sleeve is divided into three sections.

Optionally, the external thread is located on a section farthest from the sample tube.

Optionally, the axial dimension of the topmost section is 30% to 100% of the inner diameter of the first connecting section (that is, the distance between the top aluminum foil layer and the adjacent cleaning blade layer).

Optionally, the axial dimension of the topmost section is preferably 40% to 80% of the inner diameter of the first connecting section.

Optionally, the spacing between the cleaning blades of two adjacent layers is 25% to 100% of the inner diameter of the first connecting section.

Optionally, the spacing between the cleaning blades of two adjacent layers is preferably 30% to 60% of the inner diameter of the first connecting section.

Optionally, the cleaning blades are arranged layer by layer, and the plane where each layer is located is perpendicular to the axial direction of the retention cover.

Optionally, the cleaning blade includes at least two layers and is made of elastic material.

Optionally, each layer of cleaning blade is independently made of rubber or plastic.

Optionally, the thickness of each layer of cleaning blades is 0.3-2 mm.

Optionally, the cleaning blades of the same layer are multiple pieces arranged in sequence along the circumferential direction, and there are crackable gaps between adjacent pieces.

Optionally, the cleaning blades of the same layer are evenly distributed along the circumferential direction.

Optionally, the gap is cross or rice-shaped.

Optionally, the slits extend with the same width.

Optionally, the width of the slit is less than 1.5mm.

Optionally, each cleaning blade is gradually thinner at the edge adjacent to the slit.

Optionally, the gaps between the cleaning blades of different layers adopt a dislocation arrangement.

Optionally, the central angle corresponding to the outer edge of each cleaning blade in the same layer is the first angle;

The dislocation arrangement is a second angle dislocation in the circumferential direction;

And the second angle is 1/3~1/2 of the first angle.

Optionally, the sample tube cover is provided with an identification mark.

The present application also provides a method for processing a sample liquid protection device, comprising separately processing the retention cover and the sample tube cover mentioned in the sample liquid protection device of the present application;

Wherein the retention cover includes a first connection sleeve sleeved outside the sample tube along its own axial direction, and a second connection sleeve inserted into the sample tube cover;

The second connecting sleeve is a multi-segment structure, and the two adjacent segments are fixedly butted by means of hot melting or bonding; or the two adjacent segments are respectively formed by injection molding on both sides of the cleaning blade layer.

Optionally, the first connection sleeve and the second connection sleeve are fixedly butt-jointed by means of hot melting or bonding; or the first connection sleeve and the second connection sleeve are at the bottom end of the aluminum foil layer. Both sides are formed by injection molding.

The present application also provides a sample liquid containing device, including a sample tube and the sample liquid protection device as described in the present application matched with the sample tube.

The present application also provides a method for loading a sample liquid, using a sample tube and a sample liquid protection device of the present application to store the sample liquid, and the method for loading a sample includes:

After the sample tube contains the sample liquid, the mouth of the sample tube is closed with the retention cap;

When testing on the machine, the pipetting arm of the testing instrument breaks through the sealing film in the retention cover to suck the sample liquid for testing;

After the pipetting arm has removed the sample tube, the sample tube cap is installed on the retention cap.

Optionally, the sample tube cover is provided with an identification mark; before piercing the sealing film, it is determined whether the sample tube cover is installed on the retention cap according to the identification mark, and corresponding operations are performed.

Optionally, determine whether a sample tube cap is installed on the retention cap, and perform corresponding operations including:

When it is determined that there is a sample tube cover, stop sampling and prompt;

When it is judged that there is no sample cap, puncture sampling is performed.

Optionally, the identification mark is identified based on at least one of the following manners:

conductivity;

Magnetic induction;

radio signals;

image identification; and

light signal.

beneficial effect

The sample liquid protection device and its processing method, the sample liquid containing device and the sample liquid loading method of the present application have at least one of the following technical effects:

Compared with the tube caps of the prior art, the present application uses a sample tube cap and a retention cap. After the sample liquid is collected, the retention cap is used for sealed transportation. At this time, the top aluminum foil layer of the retention cap is used to isolate external pollution, and the bottom is used to isolate the external pollution. The aluminum foil at the end is used to prevent the sampling liquid from contaminating the cleaning blade when it is shaken during transportation. Of course, the sample tube cover can be optionally used at this time.

When testing on the machine, if only the retaining cap is used, the step of removing the cap is not necessary at this time. The pipetting arm of the testing instrument can directly penetrate the sealing film in the retaining cap and enter the sample tube to absorb the sample liquid. The sample liquid adsorbed on the outer wall of the pipette head falls back into the sample tube through the slits of each cleaning blade, reducing the risk of contamination to the fully automatic system. After the sampling is completed, install the sample tube cap on the retention cap to isolate the contamination from the outside world.

In addition, the sample tube cover of the present application is provided with an identification mark. If a sample tube with a retention cover is mistakenly sampled, the pipetting arm can identify the sample tube cover through the identification mark. At this time, no puncturing is performed, which can effectively Prevent pipetting arm collision damage.

Therefore, the present application reduces the workload, reduces the risk of sample contamination, and improves the degree of automation compared with the prior art for sampling by opening the lid.

Description of drawings

FIG. 1 is a partial three-dimensional assembly schematic diagram of a sample liquid protection device and a sample tube according to an embodiment of the present application;

2 is a three-dimensional cross-sectional view of a retention cover of a sample liquid protection device according to an embodiment of the present application;

3 is a schematic plan view of a combination of a sample tube cover and a retention cover of a sample liquid protection device according to an embodiment of the present application;

FIG. 4 is a partial three-dimensional assembly schematic diagram of a sample liquid protection device and a sample tube according to an embodiment of the present application;

5-6 are three-dimensional cross-sectional views of the retention cover of the sample liquid protection device according to an embodiment of the present application;

7 is a schematic diagram of the three-dimensional position matching of the cleaning blade of the retention cover of the sample liquid protection device according to an embodiment of the application;

The reference numerals in the figure are explained as follows:

100, the sample tube cover; 110, the second sealing gasket;

200, retention cover; 210, sealing film; 211, top aluminum foil layer; 212, bottom aluminum foil layer; 220, cleaning blade; 221, first blade; 221a, blade; 221b, blade; 222, second scraper; 222a, scraper; 222b, scraper; 225, gap; 225a, upper gap; 225b, lower gap; 230, first connecting sleeve; 231, internal thread; 232, installation step; 233, first gasket ;240, the second connecting sleeve; 241, the external thread;

300. Sample tube.

Embodiments of the present invention

The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, but not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present application.

It should be noted that when a component is referred to as being "connected" to another component, it can be directly connected to the other component or an intervening component may also exist. When a component is considered to be "set on" another component, it may be directly set on the other component or there may be a co-existing centered component.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field to which this application belongs. The terms used herein in the specification of the present application are for the purpose of describing specific embodiments only, and are not intended to limit the present application. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

In the high-throughput diagnostic system in the prior art, during sample processing, there is a risk of cross-contamination when opening the cap and loading samples. In addition, there is also cross-contamination during sample aspiration and tip transfer. For example, residual liquid on the outer wall of the tip and viscous samples cause There is residual liquid in the tip, the tip is dragged due to the problem sample, etc.

Referring to FIGS. 1-2 , an embodiment of the present application provides a sample liquid protection device, which is used to cooperate with the nozzle of the sample tube 300 , and the sample liquid protection device includes:

Sample tube cover 100;

The retention cover 200 is a cylindrical structure, and both ends in the axial direction are provided with connecting structures, which are respectively used for detachable sealing cooperation with the sample tube 300 and the sample tube cover 100. A pierceable sealing film 210 is provided, wherein a cleaning blade 220 is provided between the two sealing films 210 .

In the embodiment of the present application, a double cover body is used, that is, the retention cap 200 directly matched with the nozzle of the sample tube 300, and the sample tube cap 100 directly matched with the retention cap 200. The fitting method should be easy to disassemble. For example, the connection structure adopts a thread Or snap and other methods, of course, if separation is not considered, at least it should be easy to assemble, so that the packaging can be implemented in time after sampling.

The cylindrical structure does not strictly limit the cross-sectional shape, and is preferably similar to the cross-sectional shape of the sample tube 300. For the convenience of handling or slip resistance, a local convex-concave structure or texture may also be provided on the outer periphery.

A plurality of sealing films 210 are arranged in the retention cover 200, and the cleaning blade 220 is located between the two sealing films 210, which can isolate the contamination of the cleaning blade 220 from the outside; The sample inside is shaken, and the sealing film 210 can also prevent the sampling liquid from contaminating the cleaning blade 220 .

The cleaning blade 220 may remain in contact with the straw wall. During operation, the suction head breaks through each layer of the sealing film 210 and the cleaning blade 220 to suck the sample.

Since the cleaning blade 220 always keeps in contact with the wall of the suction pipe, it can remove the residual liquid on the wall of the suction pipe.

In addition, the sample tube cover 100 may not be covered before sample loading, which can simplify the process or equipment. After the sample loading is completed, if the sample liquid still needs to be stored, the sample tube cover 100 should be covered.

In order to achieve detachable sealing fit, in another embodiment, referring to FIG. 1 , the connection structure is a screw or a snap-fit structure. The thread structure is not only convenient for installation and disassembly, but also can be configured with thread type or pitch as required, so as to obtain different disassembly resistance or relative speed between the sample tube 300 and the sample tube cover 100 .

In order to ensure the tightness of the sample tube cover 100, in another embodiment, referring to FIG. 1 and FIG. 4 , the sample tube cover 100 is a cylindrical structure with one end closed, and a second sealing gasket 110 is provided inside the closed end. In the use state, the second sealing gasket 110 is clamped and fixed between the retention cover 200 and the sample tube cover 100 .

The second sealing gasket 110 can be a hollow ring or a whole-piece structure, and the second sealing gasket 110 is clamped after the sample tube cover 100 and the retaining cover 200 are screwed tightly.

In different embodiments, a portion of the sample tube cap 100 may be inserted inside the retention cap 200 , or may be located at the outer periphery of the retention cap 200 .

In a preferred embodiment, in order to facilitate assembly and placement, referring to FIG. 2 and FIG. 3 , the sample tube cover 100 is covered on the outer periphery of the retention cover 200 . In this embodiment, it can be understood that no part on the sample tube cover 100 protrudes into the interior of the retention cover 200 .

The retaining cap 200 has opposite top and bottom ends, wherein the side facing the sample tube cap 100 is the top end. The sample tube cap 100 is provided on the outer periphery of the retaining cap 200 to avoid interference with the sealing film 210 of the top layer, and is also convenient for gripping by a robot. .

Since the sample tube cap 100 is not capped in real time, if it partially protrudes into the retention cap 200, the sealing film 210 on the top layer will also be interfered, so it is necessary to reserve an installation position, and in this embodiment, the sealing film on the top layer is located on the top side. 210 can be directly covered and stretched over the top opening of retention cover 200.

In order to facilitate manually taking out the sample tube 300 and unscrewing the sample tube cover 100, in another embodiment, referring to FIG. 1 , the outer wall of the sample tube cover 100 is provided with anti-slip lines.

Personnel taking out the sample tube 300 by hand generally wear medical safety gloves, and the anti-slip pattern can prevent the sample tube 300 from slipping off; when the sample tube cover 100 is assembled and taken out uniformly, the anti-slip pattern increases the frictional force of circumferential placement and assists assembly.

The cleaning blade 220 can also be one or more layers. In order to ensure the effect of the sealing film 210 and reduce the process cost, in an embodiment, referring to FIG. 2 , the sealing film 210 is two layers, which are the top aluminum foil layer 211 and the bottom end respectively. The aluminum foil layer 212 and the cleaning blade 220 are arranged layer by layer, and there are two or more layers;

All the cleaning blades 220 are distributed between the top aluminum foil layer 211 and the bottom aluminum foil layer 212 . The aluminum foil has the characteristics of good sealing and easy to break, and is the preferred material for the sealing film 210; the top aluminum foil layer 211 is the sealing film 210 closest to the retention cover 200 in the direction of the sample tube cover 100, and the bottom aluminum foil layer 212 is the retention cover 200 facing the sample. The sealing film 210 closest to the direction of the tube 300; the cost is saved on the premise of setting two layers of aluminum foil to ensure basic functions; the cleaning blade 220 is arranged with two or more layers layer by layer, and a multi-layer setting is adopted to remove the residual liquid and Drag sample.

In order to reduce the contamination of the sample liquid, in another embodiment, referring to FIG. 1 , the side of the retention cover 200 facing the sample tube 300 is located outside the sample tube 300 , and the side facing the sample tube cover 100 is located on the sample tube cover. 100 inside.

In this embodiment, it can be understood that no part on the retention cap 200 protrudes into the interior of the retention cap 200, so as to reduce the parts contaminated by the sample liquid as much as possible.

In order to take into account the processing convenience of the sealing film 210 and the threaded structure, in another embodiment, referring to FIG. 4 and FIG. 5 , the retaining cap 200 includes a first connecting sleeve 230 sleeved on the outside of the sample tube 300 along its own axial direction, and is inserted into the second connection sleeve 240 in the sample tube cover 100;

The connection structure includes an internal thread 231 provided on the first connection sleeve 230 and an external thread 241 provided on the second connection sleeve 240; the internal thread 231 is threaded with the thread correspondingly provided on the outer peripheral wall of the sample tube 300; the external thread 241 is threaded with the sample Correspondingly provided threads on the inner peripheral wall of the pipe cover 100 form thread fit.

The top aluminum foil layer 211 is located on the end of the second connection sleeve 240 away from the first connection sleeve 230 , and the bottom aluminum foil layer 212 is located between the first connection sleeve 230 and the second connection sleeve 240 .

In another embodiment, referring to FIG. 5 , the pitch of the inner thread 231 on the first connecting sleeve 230 (the value is B) is smaller than the pitch (the value is A) of the external thread 241 on the second connecting sleeve 240 . The pitch of the internal thread 231 is relatively small (A is greater than or equal to B), and the unscrewing speed of the internal thread 231 relative to the external thread 241 is slow, which reduces the risk that the retaining cap 200 is synchronously unscrews to expose the nozzle.

In order to prevent the retaining cap 200 from being unscrewed by mistake, in another embodiment, referring to FIG. 4 , the inner thread 231 is provided with an engaging structure, and the disengagement resistance of the engaging structure is greater than the unscrewing resistance of the outer thread 241 .

In the embodiment of the present application, the engaging structure includes:

The protruding end is arranged on the thread line of the inner thread 231, and when the inner thread 231 is matched with the sample tube 300, it is at the stroke end of the thread line of the inner thread 231;

The end groove is provided on the thread line of the sample tube 300 matched with the inner thread 231, and when the inner thread 231 is matched with the sample tube 300, it is at the end of the thread line of the sample tube 300;

The disengagement resistance of the engaging structure is greater than the unscrewing resistance of the external thread 241 on the second connecting sleeve 240, which ensures that the retaining cover 200 is not unscrewed by mistake.

In another embodiment, referring to FIG. 1 and FIG. 6 , the retaining cap 200 has opposite top and bottom ends, wherein the side facing the sample tube cap 100 is the top end, and the interior of the retaining cap 200 can communicate with the area of the sample tube 300 A sampling channel is formed, and the inner diameter of the sampling channel extends from the top end to the bottom end of the same diameter or gradually expands.

In this embodiment, the inner diameter of the top end of the retention cover 200 is C, and the inner diameter of the bottom end is D; when the suction head of the sampling tube extends into the retention cover 200, since the inner diameter of the sampling channel extends with equal diameter or gradually expands (D is greater than or equal to C), It can not only prevent the retention cap 200 from trapping the stuck sample, but also prevent the sampling pipette from being blocked when the alignment is not correct.

In another embodiment, referring to FIG. 6 , the inner diameter of the sampling channel is gradually enlarged from the top end to the bottom end through a stepped structure and/or a gradual change structure.

In order to ensure the tightness of the retention cover 200, in another embodiment, referring to FIGS. 5 and 6 , the retention cover 200 is provided with an installation step 232 inside, and the installation step 232 is located at the first connection sleeve 230 and the second connection sleeve 240. the joint part;

A first sealing gasket 233 is stacked on the installation step 232 on the side facing the bottom end of the retention cap 200 . In the use state, the first sealing gasket 233 is clamped and fixed between the nozzle of the sample tube 300 and the installation step 232 .

In general, the outer diameter of the first connection sleeve 230 is larger than the outer diameter of the second connection sleeve 240 , so that after the sample tube cap 100 is capped, the outer diameter of the first connection sleeve 230 can be the same as the outer diameter of the capped sample tube cap 100 . The diameter is the same, and the outer circumference is smooth and flat.

To facilitate processing, in another embodiment, referring to FIGS. 2 and 5 , along the axial direction of the retaining cover 200 , the second connecting sleeve 240 is a multi-segment structure, and each layer of cleaning blades 220 is clamped and fixed in two adjacent segments. between. The method of butt-joint section by section can be adopted during processing.

In another embodiment, referring to FIG. 5 , the cleaning blade 220 has two layers, and the second connecting sleeve 240 is divided into three sections.

In another embodiment, referring to FIG. 5 , the external thread 241 is located on a section farthest from the sample tube 300 .

In order to prevent the cleaning blade 220 from turning up and deforming to affect the effect, in another embodiment, referring to FIG. 2 and FIG. 6 , the axial dimension of the topmost section is 30% of the inner diameter of the first connecting section (the inner diameter is C). %~100% (that is, the distance between the top aluminum foil layer 211 and the adjacent cleaning blade layer).

After the cleaning blade 220 is pierced by the suction head, it may warp upward and deform, interfere with the top sealing film 210, and affect the scraping effect, so it is preferable to retain a necessary axial space.

In another embodiment, referring to FIGS. 2 and 6 , the axial dimension of the topmost segment is preferably 40% to 80% of the inner diameter of the first connecting segment.

In order to prevent the overturning deformation of the cleaning blade 220 from affecting the effect, in another embodiment, referring to FIGS. 2 and 6 , the distance between the cleaning blades 220 of two adjacent layers (such as the first blade 221 and the second blade 222) is 25%~100% of the inner diameter of the first connecting segment (inner diameter value C). It can avoid warping and interfering with each other and affect the sticking effect with the outer wall of the straw.

In another embodiment, referring to FIG. 2 and FIG. 6 , the distance between the cleaning blades 220 of two adjacent layers (eg, the distance between the first blade 221 and the second blade 222 ) is preferably 30% of the inner diameter of the first connecting section. %~60%.

In another embodiment, referring to FIG. 2 , the cleaning blades 220 are arranged layer by layer, and the plane where each layer is located is perpendicular to the axial direction of the retention cover 200 . The axial space can be effectively utilized, making the structure more compact.

In order to enhance the cleaning effect of the cleaning blade 220 , in another embodiment, referring to FIG. 2 , the cleaning blade 220 includes at least two layers and is made of elastic material. When the cleaning blade 220 rubs against the suction head, the cleaning blade 220 elastically deforms and recovers due to its elasticity. The cleaning effect is thereby enhanced.

In another embodiment, referring to FIG. 2 , each layer of cleaning blade 220 is independently made of rubber or plastic material. That is, the materials between different layers (eg, the first blade 221 and the second blade 222 ) may be the same or different.

In another embodiment, referring to FIG. 2 , the thickness of each layer of the cleaning blade 220 is 0.3˜2 mm. Appropriate thickness can balance elasticity and cleaning effect.

In another embodiment, referring to FIG. 2 , the cleaning blades 220 of the same layer are multiple sheets arranged in sequence along the circumferential direction, and there are crackable slits 225 between adjacent sheets. For example, there are four first scrapers 221 in the upper layer, and the scrapers 221a and 221b are sequentially arranged in the circumferential direction. There are also four second scrapers 222 in the lower layer, which are scrapers 222a and 222b in sequence along the circumferential direction.

The crackable gaps 225 may be independent of each other, only abutting or adjacent to each other, or there may be fragile parts between each other, which are cracked under the action of the straw during sample loading, and are separated from each other to avoid the straw.

In another embodiment, referring to FIG. 7 , the cleaning blades 220 of the same layer (eg, the first blade 221 or the second blade 222 ) are evenly distributed along the circumferential direction. The uniform distribution can make the deformation between the sheets roughly the same, and ensure the uniformity of the cleaning effect.

In another embodiment, referring to FIG. 7 , the slit 225 generally extends along the radial direction of the retaining cover 200 , for example, in the shape of a cross or a square shape, and a single cleaning blade is correspondingly fan-shaped.

In another embodiment, referring to FIG. 7 , the slits 225 extend with the same width, and can also be widened, narrowed, or closed in advance near the radially outer end in order to adjust the deformability.

In another embodiment, referring to FIG. 7 , the width of the slit 225 is less than 1.5 mm. The smaller the width of the slit 225 is, the better the cleaning effect is, especially when there is only one layer of cleaning blade 220 .

In order to enhance the cleaning effect of the cleaning blades 220 , in another embodiment, referring to FIG. 7 , the edges of the cleaning blades 220 adjacent to the slits 225 are gradually thinner. The ease of the suction head passing through the cleaning blade 220 and the cleaning effect can be taken into consideration.

In order to enhance the cleaning effect of the cleaning blade 220 , in another embodiment, referring to FIGS. 2 and 7 , the gap 225 between the cleaning blades 220 of different layers (eg, the first blade 221 and the second blade 222 ) adopts the Misplaced arrangement.

In the staggered arrangement, when the suction head passes through the cleaning blades 220 of different layers (such as the first blade 221 and the second blade 222 ), the main focus point between the cleaning blades 220 of different layers and the outer wall of the suction head will be. Distributed at different positions in the circumferential direction, so as to avoid repeated application of force at the same position, and can enhance the cleaning effect of the cleaning blade 220 . In this case, the width restriction of the slit 225 is also relatively relaxed.

In another embodiment, referring to FIG. 7 , the central angle corresponding to the outer edge of each cleaning blade 220 in the same layer is the first angle α, the dislocation arrangement is the second angle β in the circumferential direction, and the second angle β is 1/3~1/2 of the first angle α.

For example, the scraper 222b is fan-shaped, and the corresponding central angle is 90 degrees;

The position where the upper layer slit 225a is projected onto the second blade 222 is the reference line 225a', and the extension direction of the lower layer slit 225b and the reference line 225a' include an angle of 45 degrees;

That is, in this embodiment, the second angle β is 1/2 of the first angle α.

In another embodiment, referring to FIG. 1 , FIG. 2 and FIG. 5 , an embodiment of the present application further provides a method for processing a sample liquid protection device, including processing the retention cover 200 and the sample tube cover 100 respectively;

The retention cover 200 includes a first connection sleeve 230 sleeved outside the sample tube 300 along its own axis, and a second connection sleeve 240 inserted into the sample tube cover 100;

The second connecting sleeve 240 is a multi-segment structure, and the two adjacent segments are fixedly buttted by means of hot melting or bonding; or between two adjacent segments on both sides of the cleaning blade layer (such as the Both sides) are separately injection-molded.

In the embodiment of the present application, the multi-segment structure of the second connecting sleeve 240 can be, for example, by means of hierarchical processing, stepped stamping or assembly, to assemble the cleaning blades 220 of different layers and the second connecting sleeve 240 together into one The cylindrical member is assembled into a cylindrical mechanism composed of a double-layer sealing film 210 .

In order to process the first connecting sleeve 230 and the second connecting sleeve 240, in another embodiment, referring to FIGS. 1 , 2 and 5 , the first connecting sleeve 230 and the second connecting sleeve 240 are thermally melted or bonded. or the first connection sleeve 230 and the second connection sleeve 240 are respectively formed by injection molding on both sides of the bottom aluminum foil layer 212 .

Referring to FIG. 1 , an embodiment of the present application further provides a sample liquid containing device, including a sample tube 300 and a sample liquid protection device matched with the sample tube 300 , and the sample liquid protection device can be the sample liquid protection device of the above embodiments device.

An embodiment of the present application also provides a method for loading a sample liquid. The sample tube 300 and the sample liquid protection device as shown in FIG. 1 can be used to store the sample liquid. The sample loading method includes:

After the sample tube 300 contains the sample liquid, the opening of the sample tube 300 is closed by the retention cover 200;

When testing on the machine, the pipetting arm of the testing instrument breaks through the sealing film 210 in the retention cover 200 to suck the sample liquid for testing;

After the pipetting arm removes the sample tube 300 , the sample tube cap 100 is installed on the retention cap 200 .

After the pipetting arm of the testing instrument sucks the sample liquid, the sample liquid adhering to the outer wall of the suction head falls back into the sample tube 300 under the action of each cleaning blade 220, which can reduce the risk of contamination. After sampling is completed, installing the sample tube cover 100 can be used to isolate external contamination. The method for adding liquid to the sample liquid provided in this embodiment reduces the workload, reduces the risk of sample contamination, and improves the degree of automation compared with the prior art for sampling by opening the lid.

Preferably, in another embodiment, the sample tube cover 100 is provided with an identification mark; before the sealing film 210 is pierced, it is determined whether the sample tube cover 100 is installed on the retention cap 200 according to the identification mark, and corresponding operations are performed.

The way in which the identification mark is identified may be through at least one of magnetic induction, radio signal, image mark, and optical signal.

Identify specific forms of identification, such as conductive materials, preset graphics or color blocks, barcodes, two-dimensional codes, electromagnetic induction components, and RFID tags.

Before piercing the sealing film 210, the identification mark can be used to determine whether the sample tube cover 100 exists or not, or whether the position is abnormal, etc. Similarly, identification marks can be set on the retention cover 200 and the sample tube 300 to facilitate recording, tracking or mutual match.

After identifying the signal, it can be output in various forms, and follow-up operations can be performed according to preset logical conditions, providing a basis for automated processing.

Preferably, in another embodiment, determining whether the sample tube cover 100 is installed on the retaining cover 200, and implementing the corresponding operation includes:

When it is determined that the sample tube cover 100 exists, stop sampling and prompt;

When it is determined that there is no sample tube cap 100, puncture sampling is performed.

If it is determined that the sample tube cover 100 exists, the first signal will be fed back to the control system to stop sampling, and the pipetting arm will not be punctured.

The determination process can be carried out by the data processing device of the detection device itself, or by adding a peripheral identification device, which is carried out through the peripheral identification device, and then communicates with the detection device. The above control system can be understood as the data processing of the detection device itself. The specific form of the device can be a common controller in the prior art (including but not limited to a microprocessor unit, an programmable logic controller, etc.), and of course necessary peripheral electronic components and input and output devices can be configured. If it is determined that there is no sample tube cover 100, the second signal is fed back to the control system, and then the pipetting arm is controlled to perform breakthrough sampling.

When it is determined that the sample tube cover 100 exists, sampling needs to be suspended on the one hand, and relative positioning and calibration of the pipetting arm and the sample tube 300 can also be performed based on the sample tube cover 100 . It can be understood that, the calibration positioning method may be that the pipetting arm is directly positioned and calibrated with the sample tube 300 , or the pipetting arm may be positioned and calibrated through the sample liquid protection device and the sample tube 300 . The calibration method can be calibrated by moving the pipetting arm, or can be calibrated by moving the sample tube 300 . The positioning method may be positioning by infrared detection of the contour position, or identification and positioning may be performed by the mark fixedly provided on the sample tube 300 and the sample liquid protection device.

The technical features of the above embodiments can be combined arbitrarily. In order to simplify the description, all possible combinations of the technical features in the above embodiments are not described. However, as long as there is no contradiction in the combination of these technical features, all It is considered to be the range described in this specification. When the technical features of different embodiments are embodied in the same drawing, it can be considered that the drawing also discloses the combination examples of the various embodiments involved.

The above examples only represent several embodiments of the present application, and the descriptions thereof are relatively specific and detailed, but should not be construed as a limitation on the scope of the patent application. It should be noted that, for those skilled in the art, without departing from the concept of the present application, several modifications and improvements can be made, which all belong to the protection scope of the present application.

Claims

A sample liquid protection device for matching with the nozzle of a sample tube, characterized in that the sample liquid protection device includes:

Sample tube cover;

The retention cover is a cylindrical structure, and both ends in the axial direction are provided with connecting structures, which are respectively used for detachable sealing cooperation with the sample tube and the sample tube cover, and the interior of the retention cover includes spaced arrangements The multi-channel pierceable sealing film is provided with a cleaning blade between the two sealing films.
The sample liquid protection device according to claim 1, wherein the connection structure is a thread or a snap-fit structure.
The sample liquid protection device according to claim 1, wherein the sample tube cover is a cylindrical structure with one end closed, and a second sealing gasket is arranged inside the closed end, and in a use state, the second sealing gasket is A gasket is clamped and secured between the retention cap and the sample tube cap.
The sample liquid protection device according to claim 1, wherein the sample tube cover is provided on the outer periphery of the retention cap.
The sample liquid protection device according to claim 1, wherein the outer wall of the sample tube cover is provided with anti-skid lines.
The sample liquid protection device according to claim 1, wherein the sealing film has two layers, which are a top aluminum foil layer and a bottom aluminum foil layer, and the cleaning blades are arranged layer by layer, and there are two layers or two layers. above layer;

All cleaning blades are distributed between the top foil layer and the bottom foil layer.
The sample liquid protection device according to claim 1, wherein the side of the retention cover facing the sample tube is located outside the sample tube, and the side facing the sample tube cover is located on the sample tube cover inside.
The sample liquid protection device according to claim 6, wherein the retaining cap comprises a first connecting sleeve covering the outside of the sample tube along its axial direction, and a second connecting sleeve inserted into the sample tube cap. connecting sleeve;

The connection structure includes an inner thread arranged on the first connection sleeve and an outer thread arranged in the second connection sleeve;

The top aluminum foil layer is located at one end of the second connection sleeve away from the first connection sleeve, and the bottom aluminum foil layer is located between the first connection sleeve and the second connection sleeve.
The sample liquid protection device according to claim 8, wherein the thread pitch of the inner thread is smaller than the thread pitch of the outer thread.
The sample liquid protection device according to claim 8, wherein the inner thread has an engaging structure, and the disengagement resistance of the engaging structure is greater than the unscrewing resistance of the outer thread.
The sample liquid protection device according to claim 1, wherein the retaining cap has opposite top and bottom ends, wherein the side facing the sample tube cap is the top end, and the interior of the retaining cap is the same as the top end. The connectable area of the sample tube constitutes a sampling channel, and the inner diameter of the sampling channel extends equally or gradually expands from the top end to the bottom end.
The sample liquid protection device according to claim 11, wherein the inner diameter of the sampling channel is gradually enlarged from the top end to the bottom end through a stepped structure and/or a gradual structure.
The sample liquid protection device according to claim 8, wherein an installation step is provided inside the retention cover, and the installation step is located at the joint portion of the first connection sleeve and the second connection sleeve;

The installation step is stacked with a first sealing gasket on the side facing the bottom end of the retention cap, and in a use state, the first sealing gasket is clamped and fixed between the nozzle of the sample tube and the installation step.
The sample liquid protection device according to claim 8, characterized in that, along the axial direction of the retaining cap, the second connecting sleeve is a multi-segment structure, and each layer of cleaning blades is clamped and fixed between two adjacent segments.
The sample liquid protection device according to claim 14, wherein the cleaning blade has two layers, and the second connecting sleeve is divided into three sections.
The sample liquid protection device according to claim 15, wherein the outer thread is located on a section farthest from the sample tube.
The sample liquid protection device according to claim 16, wherein the axial dimension of the topmost section is 30%-100% of the inner diameter of the first connecting section.
The sample liquid protection device according to claim 16, wherein the axial dimension of the topmost section is preferably 40%-80% of the inner diameter of the first connecting section.
The sample liquid protection device according to claim 16, wherein the spacing between the cleaning blades of two adjacent layers is 25% to 100% of the inner diameter of the first connecting section.
The sample liquid protection device according to claim 16, wherein the distance between the cleaning blades of two adjacent layers is preferably 30% to 60% of the inner diameter of the first connecting section.
The sample liquid protection device according to claim 1, wherein the cleaning blades are arranged layer by layer, and the plane where each layer is located is perpendicular to the axial direction of the retention cover.
The sample liquid protection device according to claim 21, wherein the cleaning blade comprises at least two layers and is made of elastic material.
The sample liquid protection device according to claim 21, wherein each layer of the cleaning blade is independently made of rubber or plastic.
The sample liquid protection device according to claim 21, wherein the thickness of each layer of the cleaning blade is 0.3-2 mm.
The sample liquid protection device according to claim 21, wherein the cleaning blades of the same layer are a plurality of sheets arranged in sequence along the circumferential direction, and there are crackable gaps between adjacent sheets.
The sample liquid protection device according to claim 25, wherein the cleaning blades of the same layer are evenly distributed along the circumferential direction.
The sample liquid protection device according to claim 26, wherein the slit is in the shape of a cross or a square shape.
The sample liquid protection device according to claim 25, wherein the slits extend with the same width.
The sample liquid protection device according to claim 25, wherein the width of the slit is less than 1.5 mm.
The sample liquid protection device according to claim 25, wherein each cleaning blade is gradually thinner at an edge adjacent to the slit.
The sample liquid protection device according to claim 25, wherein the gaps between the cleaning blades of different layers are arranged in a dislocation manner.
The sample liquid protection device according to claim 31, wherein the central angle corresponding to the outer edge of each cleaning blade in the same layer is the first angle;

The dislocation arrangement is a second angle dislocation in the circumferential direction;

And the second angle is 1/3~1/2 of the first angle.
The method for processing a sample liquid protection device according to any one of claims 1 to 32, comprising processing the retention cap and the sample tube cap respectively, characterized in that;

Wherein the retention cover includes a first connection sleeve sleeved outside the sample tube along its own axial direction, and a second connection sleeve inserted into the sample tube cover;

The second connecting sleeve is a multi-segment structure, and the two adjacent segments are fixedly butted by means of hot melting or bonding; or the two adjacent segments are respectively formed by injection molding on both sides of the cleaning blade layer.
The method for processing a sample liquid protection device according to claim 33, wherein the first connection sleeve and the second connection sleeve are fixedly butt-jointed by means of heat fusion or bonding; The connection sleeve and the second connection sleeve are respectively formed by injection molding on both sides of the aluminum foil layer at the bottom end.
A sample liquid containing device is characterized by comprising a sample tube and the sample liquid protection device according to any one of claims 1 to 32, which is matched with the sample tube.
A method for loading a sample liquid, characterized in that a sample tube and the sample liquid protection device according to any one of claims 1 to 32 are used to store the sample liquid, and the method for loading a sample comprises:

After the sample tube contains the sample liquid, the mouth of the sample tube is closed with the retention cap;

When testing on the machine, the pipetting arm of the testing instrument breaks through the sealing film in the retention cover to suck the sample liquid for testing;

After the pipetting arm has removed the sample tube, the sample tube cap is installed on the retention cap.
The sample loading method according to claim 36, wherein the sample tube cover is provided with an identification mark; before piercing the sealing film, it is determined whether a sample tube cover is installed on the retention cover according to the identification mark, and the Operate accordingly.
The sample loading method according to claim 37, wherein determining whether a sample tube cover is installed on the retention cover, and implementing a corresponding operation comprises:

When it is determined that there is a sample tube cover, stop sampling and prompt;

When it is judged that there is no sample cap, puncture sampling is performed.
The sample loading method according to claim 37, wherein the identification mark is identified based on at least one of the following ways:

conductivity;

Magnetic induction;

radio signals;

image recognition; and

light signal.