WO2022211475A1 - Fermeture d'un récipient de stockage d'échantillon liquide et dispositif de stockage d'échantillon liquide la comprenant - Google Patents

Fermeture d'un récipient de stockage d'échantillon liquide et dispositif de stockage d'échantillon liquide la comprenant Download PDF

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Publication number
WO2022211475A1
WO2022211475A1 PCT/KR2022/004479 KR2022004479W WO2022211475A1 WO 2022211475 A1 WO2022211475 A1 WO 2022211475A1 KR 2022004479 W KR2022004479 W KR 2022004479W WO 2022211475 A1 WO2022211475 A1 WO 2022211475A1
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WO
WIPO (PCT)
Prior art keywords
body part
storage container
blocking
closure
main body
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Application number
PCT/KR2022/004479
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English (en)
Inventor
Yong Rae Lee
Original Assignee
Seegene, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seegene, Inc. filed Critical Seegene, Inc.
Publication of WO2022211475A1 publication Critical patent/WO2022211475A1/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D51/00—Closures not otherwise provided for
    • B65D51/002—Closures to be pierced by an extracting-device for the contents and fixed on the container by separate retaining means

Definitions

  • the present invention relates to a closure of a liquid sample storage container capable of allowing entry of a pipette without removing the closure, and a liquid sample storage device including the same.
  • Nucleic acid amplification reaction well known as polynucleotide chain reaction (PCR) includes repeated cycles of double-stranded DNA denaturation, annealing of the oligonucleotide primers to DNA templates, and extension/elongation of the primers with the DNA polymerase (Mullis et al., U.S. Patent Nos. 4,683,195, 4,683,202, and 4,800,159; Saiki et al., (1985) Science 230, 1350-1354).
  • PCR polynucleotide chain reaction
  • nucleic acid amplification devices for performing nucleic acid amplification reactions have been developed.
  • An example of a nucleic acid amplification device may be equipped with a container containing a sample solution containing a template nucleic acid in one reaction chamber. A nucleic acid amplification reaction is performed by repeatedly heating and cooling the container.
  • Fluorescence detection devices for reaction analysis in particular, various devices or apparatuses for analyzing a nucleic acid amplification reaction in real time have been known.
  • the optical device at the top of Biorad's real-time PCR device CFX96 includes an optical shuttle equipped with six light sources and six detectors. The optical shuttle moves along the X- and Y-axes while real-time detecting the fluorescence generated in the nucleic acid amplification reaction (see U.S. Patent No. 8,236,504).
  • FIG. 1 shows the optical shuttle, i.e., optical module 10, of the CFX96 device.
  • the reaction vessel 13 is positioned in the sample well 14 provided in the thermal block 15, and a nucleic acid amplification reaction proceeds in the reaction vessel 13. Fluorescence generated from the reaction vessel 13 is measured at regular time intervals while the nucleic acid amplification reaction is in progress.
  • excitation light 22 is generated from the light source 11, passes through the filter 17 and the lens 18, and then is provided to the reaction vessel 13.
  • Reflection light from the reaction mixture in the reaction vessel 13 is guided to the photodiode (i.e., photodetector 12) by the beam splitter 21, passes through the filter 19 and lens 20, and is then detected by the photodiode 12.
  • the photodiode i.e., photodetector 12
  • the reaction vessel used to perform the nucleic acid amplification reaction process or its pre-processing process is prepared to open and close the closure, so that storage and transport are performed with the closure closed, and the sample is injected or extracted with the closure open.
  • opening and closing the closure is typically manually conducted so that contamination may occur during the course and, if the closure is kept open for a long time to reduce such issue, the sample may evaporate or a foreign body may enter the reaction vessel.
  • the device is placed on the floor with the closure open for working and, in this case, its bottom may be contaminated.
  • Korean Patent Application Publication No. 10-2019-0007094 discloses a nucleic acid amplification device and a fluorescence detection device.
  • a closure for a liquid sample storage container capable of preventing evaporation or contamination of a sample while allowing entry of a pipette into the storage container with a closure coupled without the need for opening and closing the closure and a liquid sample storage device including the same.
  • a closure for a liquid sample storage container in which a blocking member having a pierceable blocking part allowing entry of a pipette may remain fixed to a coupling member coupled to the storage container and a liquid sample storage device including the same.
  • a closure of a liquid sample storage container capable of being attached to an upper opening of a storage container having a chamber for storing a liquid sample, may comprise a coupling member coupled to an upper portion of a cylindrical main body part of the storage container and a blocking member blocking the upper opening of the main body part of the storage container.
  • the coupling member may include a first body part coupled to an outer surface of the upper portion of the main body part of the storage container, a second body part having an outer radius smaller than an inner radius of the main body part of the storage container, and a closure part connecting an upper portion of the first body part and an upper portion of the second body part.
  • the blocking member may include a side part interposed between the main body part of the storage container and the second body part, and a pierceable blocking part connected to a lower portion of the side part, prepared to have elastic restorative force, and permitting entry of a pipette while keeping the upper opening of the chamber closed while not entered by the pipette, and permitting entry of a pipette while the pierceable blocking part is pressurized by the pipette.
  • the coupling member has a first fixing part which fixes the side part of the blocking member and is formed to fix the side part of the blocking member, is formed on an outer circumferential surface of the second body part of the coupling member.
  • the blocking member may havehas a second fixing part which is fixed to the first fixing part by a hooking structure of a groove and a protrusion may beand is formed on an inner circumferential surface of the side part of the blocking member.
  • the first fixing part may be prepared in a ring shape circumferentially protruding from the outer circumferential surface of the second body part.
  • the coupling member may include the first body part shaped as a cylinder receiving an upper portion of the main body part of the storage container, a closure part connected to an upper portion of the first body part and forming an opening permitting a movement of the pipette entering/exiting a chamber of the storage container and the second body part bending and extending downward from around the opening of the closure part.
  • the blocking member may further include a seating part which is radially bent outward of the main body part of the storage container from an upper portion of the side part and is seated on an upper end of the main body part of the storage container.
  • the seating part may be prepared to be compressed between a lower surface of the closure part of the coupling member and an upper surface of the main body part of the storage container and elastically deformed as the coupling member is coupled to the main body part of the storage container.
  • a lower surface of the seating part, contacting an upper surface of the main body part may be positioned higher than a lower surface of the first fixing part of the second body part.
  • the pierceable blocking part may include one or more cutouts passing through a center of the main body part of the storage container.
  • the cutouts may be prepared by complete cutting to pass through an upper surface and lower surface of the pierceable blocking part.
  • the cutouts may be prepared by incomplete cutting to extend from an upper surface of the pierceable blocking part and not to pass through a lower surface of the pierceable blocking part.
  • a spiral shape corresponding to a spiral shape formed on the outer circumferential surface of the main body part of the storage container may be prepared on the inner circumferential surface of the first body part of the coupling member to be screwed with the storage container.
  • An outer circumferential surface of the side part of the blocking member may be spaced apart from an inner circumferential surface of an opening of the main body part of the storage container by a predetermined distance.
  • the side part of the blocking member may include a portion having an outer diameter larger than an inner diameter of the upper opening of the main body part of the storage container to be inserted into the upper opening of the main body part and elastically deformed radially inward of the main body part of the storage container.
  • the side part of the blocking member may include an upper portion with an outer diameter equal to or smaller than the inner diameter of the upper opening of the main body part of the storage container and a lower portion with an outer diameter larger than the inner diameter of the upper opening of the main body part of the storage container.
  • the closure may further comprise a film detachably attached to an upper surface of the closure part of the coupling member.
  • the side part, the pierceable blocking part, and the first fixing part may be integrally formed.
  • the blocking member may include a first blocking member including a ring-shaped first side part interposed between the main body part of the storage container and the second body part and a first pierceable blocking part connected to a lower portion of the first side part, prepared to have an elastic restorative force, and permitting entry of the pipette while keeping the upper opening of the chamber closed while not entered by the pipette, and permitting entry of a pipette while the pierceable blocking part is pressurized by the pipette and a second blocking member including a second pierceable blocking part spaced apart from an upper portion of the first pierceable blocking part in a center area of the main body part of the storage container, prepared to have an elastic restorative force and, permitting entry of the pipette while keeping the upper opening of the chamber closed while not entered by the pipette, and permitting entry of a pipette while the pierceable blocking part is pressurized by the pipette.
  • a first blocking member including a ring-shaped first side
  • the second blocking member may include a ring-shaped second side part interposed between the main body part of the storage container and the first side part and the second pierceable blocking part connected to a lower portion of the second side part.
  • a closure of a liquid sample storage container capable of being attached to an upper opening of a storage container having a chamber for storing a liquid sample, may comprise a coupling member coupled to an upper portion of a cylindrical main body part of the storage container and a blocking member blocking the upper opening of the main body part of the storage container.
  • the coupling member may include a body part screwed to an upper portion of the main body part of the storage container and having an insertion hole for fixing one side of the blocking member.
  • the blocking member may include an insertion protrusion inserted to the insertion hole, a seating part seated on an upper end of the main body part of the storage container, and a pierceable blocking part prepared to have elastic restorative force, and permitting entry of the pipette while keeping the upper opening of the chamber closed while not entered by the pipette, and permitting entry of a pipette while the pierceable blocking part is pressurized by the pipette.
  • a liquid sample storage device may comprise a storage container having a chamber for storing a liquid sample and a closure capable of being attached to an upper opening of the storage container.
  • the storage container may include a cylindrical main body part.
  • the closure may include a coupling member coupled to an upper portion of the main body part of the storage container and a blocking member blocking the upper opening of the main body part of the storage container.
  • the coupling member may include a first body part coupled to an outer surface of the upper portion of the main body part of the storage container, a second body part having an outer radius smaller than an inner radius of the main body part of the storage container, and a cap part connecting an upper portion of the first body part and an upper portion of the second body part.
  • the blocking member may include a side part interposed between the main body part of the storage container and the second body part and a pierceable blocking part connected to a lower portion of the side part, prepared to have elastic restorative force, and permitting entry of a pipette while keeping the upper opening of the chamber closed while not entered by the pipette, and permitting entry of a pipette while the pierceable blocking part is pressurized by the pipette.
  • the coupling member has a first fixing part which fixes the side part of the blocking member and is formed to fix the side part of the blocking member, is formed on an outer circumferential surface of the second body part of the coupling member.
  • a closure for a liquid sample storage container which may reduce contamination caused when repeating the opening and closing of the closure or placing on the floor with the closure open while increasing user convenience by allowing the liquid sample to be taken with the closure closed, without the need for opening or closing the closure to take the liquid sample, and a liquid sample storage device including the same.
  • the embodiments eliminate the need for the worker to open or close the closure, allowing for automation of the device.
  • the blocking member may remain fixed to the coupling member, rendering it possible to easily attach the closure to the storage container and to prevent the blocking member from remaining stuck to the storage container when removing the closure from the storage container.
  • the embodiments may adopt a screw fastening structure, preventing the closure from escaping off even when being subjected to a significant change in air pressure e.g., when it is transported by airplane.
  • embodiments may prevent contamination of the blocking member when stored with the closure open.
  • the embodiments may prevent the blocking member from departing out of its position when the blocking member is penetrated by the pipette.
  • the embodiments include a detachable film on the upper surface of the closure, fundamentally preventing contamination of the pierceable blocking part.
  • the embodiments include a dual-structure pierceable blocking part, rendering it possible to wipe off the reagents remaining on the tip of the pipette while effectively preventing evaporation and scattering.
  • FIG. 1 is a schematic diagram illustrating the operation of an optical module.
  • FIG. 2 is a view illustrating a configuration of a fluorescence detection device.
  • FIG. 3 is a view illustrating a liquid sample storage device with a closure according to an embodiment of the disclosure.
  • FIG. 4 is a cross-sectional view illustrating a liquid sample storage device with a closure according to a first embodiment of the disclosure.
  • FIG. 5 is an enlarged view of a closure attached portion of FIG. 4.
  • FIG. 6 is a view illustrating a state in which a pipette penetrates a closure.
  • FIG. 7 are views illustrating embodiments of a pierceable blocking part.
  • FIG. 8 is a view illustrating a liquid sample storage device with a closure according to a second embodiment of the disclosure.
  • FIG. 9 is a view illustrating a liquid sample storage device with a closure according to a third embodiment of the disclosure.
  • FIG. 10 is a view illustrating a liquid sample storage device with a closure according to a fourth embodiment of the disclosure.
  • FIGS. 11 to 13 are views illustrating a liquid sample storage device with a closure according to other embodiments of the disclosure.
  • Such denotations as “first,” “second,” “A,” “B,” “(a),” “(b),” “(i),” and “(ii)” may be used in describing the components of the present invention. These denotations are provided merely to distinguish a component from another, and the essence of the components is not limited by the denotations in light of order or sequence.
  • a component is described as “connected,” “coupled,” or “linked” to another component, the component may be directly connected or linked to the other component, but it should also be appreciated that other components may be “connected,” “coupled,” or “linked” between the components.
  • nucleic acid amplification device refers to a device that performs a nucleic acid amplification reaction for amplifying a nucleic acid having a specific nucleotide sequence.
  • it refers to a device that performs a nucleic acid amplification reaction through temperature control.
  • the nucleic acid amplification device may perform a denaturing step, an annealing step, and an extension (or amplification) step.
  • the denaturing step is the step of separating double-stranded DNA into single-stranded DNA by heating a solution containing a sample and reagent containing double-stranded DNA, which is a template nucleic acid, to a specific temperature, e.g., about 95°C.
  • the annealing step is the step of forming a partial DNA-primer complex by providing an oligonucleotide primer having a nucleotide sequence complementary to the nucleotide sequence of the nucleic acid to be amplified and cooling it, together with the separated single-stranded DNA to a specific temperature, e.g., 60°C to bind the primer to a specific nucleotide sequence of the single-stranded DNA.
  • the extension step is the step of forming double-stranded DNA based on the primer of the partial DNA-primer complex by DNA polymerase by maintaining the solution at a specific temperature, e.g., 72°C, after the annealing step.
  • the nucleic acid amplification device may simultaneously perform the annealing step and the extension step. In this case, the nucleic acid amplification device may complete the first cycle by performing two steps consisting of a denaturation step and an annealing/extension step.
  • FIG. 2 is a view illustrating a configuration of a fluorescence detection device.
  • a fluorescence detection device 100 includes a light source 10, a beam splitter 20, a pressure lid 30, a thermal block 40, a thermal block housing 50, a detector 60, and a case 70.
  • the light source 10 emits light to excite the fluorescent material included in the sample.
  • the light emitted by the light source 10 may be referred to as an excitation beam.
  • the light emitted from the sample may be referred to as an emission beam.
  • the path of the excitation beam from the light source 10 may be referred to as an excitation path.
  • the path of the emission beam from the sample may be referred to as an emission path.
  • the light source 10 may be a light emitting diode (LED).
  • the beam splitter 20 reflects and transmits the light incident from the light source 10.
  • the light transmitted through the beam splitter 20 passes through the hole of the pressure lid 30 to the thermal block 40.
  • the beam splitter 20 reflects and transmits the light emitted from the sample.
  • the light reflected by the beam splitter 20 arrives at the detector 60.
  • the pressure lid 30 provides pressure to the reaction vessels received in the thermal block 40.
  • the pressure lid 30 may contact the covers of the reaction vessels and press the covers of the reaction vessels, providing pressure to the reaction vessels.
  • the pressure lid 30 may maintain a high temperature.
  • the pressure lid 30 may include a heat plate (not shown) to maintain a temperature of 105 °C.
  • the pressure lid 30 includes a plurality of holes.
  • the holes of the pressure lid 30 are formed in positions corresponding to the holes of the thermal block 40.
  • the thermal block 40 receives the reaction vessels.
  • the thermal block 40 may include a plurality of holes, and reaction vessels may be positioned in the holes.
  • the reaction vessels may contain samples, from which fluorescence is emitted.
  • the thermal block 40 may be an electrically conductive material. When the thermal block 40 comes in contact with the reaction vessels, heat may be transferred from the thermal block 40 to the reaction vessel.
  • the thermal block may be formed of a metal, such as aluminum, gold, silver, nickel, or copper.
  • the thermal block housing 50 may accommodate the thermal block 40, a heat transfer element (not shown), a heat radiating plate (not shown), and the like.
  • the heat transfer element increases or decreases the temperature of the thermal block 40.
  • the heat transfer element may be disposed below the thermal block 40 and contact the thermal block 40, transferring heat to the thermal block 40.
  • the heat transfer element may be a Peltier element.
  • the heat radiating plate is positioned under the heat transfer element to radiate the heat generated from the heat transfer element.
  • the detector 60 detects signals. Specifically, the detector 60 detects the fluorescence generated from the samples.
  • the detector 60 may be, e.g., a CCD (Charge Coupled device), CMOS (Complementary Metal Oxide Semiconductor field effect transistor) or photodiode.
  • FIG. 3 is a view illustrating a liquid sample storage device with a closure according to an embodiment of the disclosure.
  • FIG. 4 is a cross-sectional view illustrating a liquid sample storage device with a closure according to a first embodiment of the disclosure.
  • FIG. 5 is an enlarged view of a closure attached portion of FIG. 4.
  • FIG. 6 is a view illustrating a state in which a pipette penetrates a closure.
  • a liquid sample storage container 200 includes a cylindrical main body part 210 and a chamber 220 formed in the main body part 210 to store a liquid sample.
  • a closure coupling part 211 coupled to the closure 100 of the liquid sample storage container is formed on the upper outer circumferential surface of the main body part 210.
  • the chamber 220 may be provided to receive a predetermined amount of liquid sample, such as 0.6ml or 0.3ml.
  • the chamber 220 may be provided to have a radius decreasing downward and to have a U-shaped cross-section in the axial direction.
  • a pipette 300 entering through the upper opening of the storage container 200 may suck a small amount of the liquid sample stored on the bottom surface of the chamber 220.
  • the pipette 300 may approach an area 0.7 mm away from the bottom surface of the chamber 220 to suck the liquid sample.
  • the closure 100 of the liquid sample storage container may include a coupling member 110 coupled to an upper portion of the storage container 200 and a blocking member 120 for blocking the upper opening of the main body part 210 of the storage container.
  • the coupling member 110 may include a first body part 111 coupled to an outer surface of the upper portion of the main body part 210 of the storage container, a second body part 113 having an outer radius smaller than an inner radius of the main body part 210 of the storage container, and a closure part 112 connecting an upper portion of the first body part 111 and an upper portion of the second body part 113.
  • the first body part 111 may include a cylindrical shape to receive the upper portion of the cylindrical main body part 210 of the storage container 200 thereinside.
  • the upper outer circumferential surface of the main body part 210 of the storage container 200 i.e., the closure coupling part 211, has a spiral shape, and the inner circumferential surface of the first body part 111 of the coupling member has a spiral shape corresponding thereto, so that the closure 100 of the liquid sample storage container may be screwed to the liquid sample storage container 200.
  • a simple plug-type closure attaching structure may be easily open by external force or internal pressure and, particularly in a low-pressure environment, e.g., upon air transport, it may be easily unsealed.
  • a handle portion 111a may be provided on the outer circumferential surface of the first body part 111.
  • the handle portion 111a may be provided in a continuous groove shape and it helps to open the closure with less force by increasing the friction coefficient.
  • the closure part 112 may extend inwardly from the upper portion of the first body part 111.
  • the closure part 112 may form an opening 112a communicating with the chamber 220 of the storage container.
  • the pipette 300 may enter and exit through the opening 112a of the closure part.
  • the second body part 113 may be bent downward around the opening 112a of the closure part 112.
  • the second body part 113 may extend in the axial direction of the main body part 210 of the storage container to guide the movement of the pipette 300 entering and exiting the chamber 220 of the storage container.
  • the second body part 113 may extend downward while remaining in the same inner diameter.
  • the length of the second body part 113 may be such that the lower end of the second body part 113 is positioned below the upper end of the main body part 210 of the storage container, with the closure 100 attached to the liquid sample storage container 200.
  • the first body part 111, the closure part 112, and the second body part 113 may be integrally formed.
  • the coupling member 110 may be divided into the first body part 111, the closure part 112, and the second body part 113 depending on its function or position.
  • the blocking member 120 may include a side part 121 interposed between the main body part 210 of the storage container and the second body part 113, a pierceable blocking part 122 connected to a lower portion of the side part 121 to block the upper opening of the chamber 220, and a seating part 123 radially bent outward of the main body part 210 of the storage container from an upper portion of the side part 121 and seated on an upper end of the main body part 210 of the storage container.
  • the side part 121 may be provided in a cylinder or ring shape.
  • the pierceable blocking part 122 may be provided in a disk shape connected inwardly to the lower end of the side part 121.
  • the seating part 123 may be provided in the shape of a circular flange extending outward from the upper end of the side part 121.
  • the side part 121, the pierceable blocking part 122, and the seating part 123 may be integrally formed.
  • the blocking member 120 may be divided into the side part 121, the pierceable blocking part 122, and the seating part 123 depending on its function or position.
  • the blocking member 120 may be formed of a material that may be elastically deformed.
  • the blocking member 120 may be formed of a material that may be stored for a long time at a low temperature of minus 20 degrees or more.
  • the blocking member 120 may be formed of a material, such as rubber and elastomer.
  • the elastomer may be a high-molecular synthetic resin that has both the advantages of rubber that stretches when an external force is applied and the advantages of plastics that may be easily processed and may prevent leakage or evaporation of the liquid sample in the chamber 220, with its high restorative force.
  • the length of the side part 121 may be such that the upper end of the side part 121 is positioned higher than the upper end of the main body part 210 of the storage container 200 and the lower end thereof is positioned lower than the lower end of the second body part 113, with the closure 100 attached.
  • the side part 121 may be provided to cross the upper surface of the main body part 210 in the upper and lower directions.
  • the seating part 123 may be prepared to be compressed between a lower surface of the closure part 112 of the coupling member 110 and an upper surface of the main body part 210 of the storage container 200 and elastically deformed as the closure 100 is coupled to the storage container 200.
  • the blocking member 120 may be prevented from deforming or departing off into the inside of the main body part 210 of the storage container by the force generated downward when the pipette 300 penetrates the pierceable blocking part 122.
  • the seating part 123 may serve as a sealing member that may prevent the liquid sample in the chamber 220 from leaking out the closure 100 of the liquid sample storage container.
  • the seating part 123 may function as a sealing ring that is elastically deformable between the main body part 210 and the closure part 112, sealing off between the main body part 210 of the liquid sample storage container and the coupling member 110.
  • the outer diameter or outer radius of the side part 121 may be equal to or slightly smaller than the inner diameter or inner radius of the main body part 210 of the storage container.
  • the outer diameter of the side part 121 may be smaller than the inner diameter of the main body part 210 of the storage container in the range of 0.05 mm or less.
  • the closure 100 of the liquid sample storage container may be provided to allow the coupling member 110 and the blocking member 120 to remain coupled to each other.
  • the closure 100 of the liquid sample storage container may address inconvenience of coupling the blocking member 120 and the coupling member 110 when coupling the closure 100 of the liquid sample storage container to the main body part 210 of the liquid sample storage container and the issue that the blocking member 120 remains coupled to the main body part 210 of the liquid sample storage container or escapes off the coupling member 110 when removing the closure 100 of the liquid sample storage container.
  • a first fixing part 113a may be formed on the outer circumferential surface of the second body part 113 of the coupling member to fix the blocking member 120.
  • the first fixing part 113a may be provided to fix the blocking member 120 by a physical change, such as a shape change or surface material change.
  • the shape change may include formation of recesses or protrusions.
  • the surface material change may include surface treatment or application of an adhesive layer that may increase friction.
  • a second fixing part 121a may be formed on the inner circumferential surface of the side part 121 of the blocking member to be fixed to the first fixing part 113a by a hooking structure.
  • the second fixing part 121a may be provided to fix the blocking member 120 by a physical change, such as a change in shape or a change in surface material.
  • the shape change may include formation of recesses or protrusions.
  • the surface material change may include surface treatment or application of an adhesive layer that may increase friction.
  • the first fixing part 113a may be a ring-shaped coupling groove circumferentially recessed from the outer circumferential surface of the second body part 113.
  • the second fixing part 121a may be a fitting protrusion that is circumferentially protrude from the inner circumferential surface of the side part 121 and fitted to the first fixing part 113a.
  • the blocking member 120 may remain coupled to the blocking member 120.
  • the first fixing part may be a ring-shaped fitting protrusion that circumferentially protrudes from the outer circumferential surface of the second body part 113.
  • the second fixing part may be a fitting groove that is circumferentially recessed from the inner circumferential surface of the side part 121 and fitted over the first fixing part.
  • the length of the side part 121 may be such that the upper end of the side part 121 is positioned higher than the upper end of the main body part 210 of the storage container 200 and the lower end thereof is positioned lower than the lower end of the second body part 113.
  • the upper end of the first fixing part 113a may be positioned lower than the upper end of the main body part 210 of the storage container.
  • the lower surface of the seating part 123 in contact with the upper surface of the main body part 210 may be positioned higher than the first fixing part 113a of the second body part 113, with the coupling member 110 coupled to the main body part 210 of the storage container.
  • the pierceable blocking part 122 of the blocking member may be formed of a material that may be elastically deformed. Accordingly, it is possible to prevent evaporation of the liquid sample in the chamber 220 of the storage container 200, while in storage and in use in the device, by the elastic restorative force, even when the pierceable blocking part 122 is cut out.
  • the pierceable blocking part 122 may be cut by the pressure applied when entered by the pipette 300 to allow entry of the pipette 300.
  • the tip of the pipette may be formed sharp enough to cut the pierceable blocking part 122.
  • the pierceable blocking part 122 may have a pre-cut portion in the center thereof, to allow entry of the pipette 300.
  • the pipette 300 may easily enter the chamber 220 of the storage container 200 regardless of the shape of the pipette tip.
  • the pierceable blocking part 122 may prevent entry of an external contaminant into the chamber 202 of the liquid sample storage container through the upper opening of the main body part 210 of the storage container or exit of the liquid sample from the chamber 220 of the liquid sample storage container. Further, the pierceable blocking part 122 may be elastically restored when the pipette 300 retracts after sucking the liquid sample, wiping off the liquid sample left on the tip of the pipette 300. In this case, the excess liquid sample wiped off by the pierceable blocking part 122 may be attached on the lower surface of the pierceable blocking part 122 or drop in the chamber 220, but does not leak out through the cutout 122a of the pierceable blocking part 122.
  • FIG. 7 are views illustrating embodiments of a pierceable blocking part 122.
  • the pierceable blocking part 122 may include one or more cutouts 122a passing through the center of the main body part 210 of the storage container.
  • the shape of the cutout 122a passing through the center of the second body part 113 may be provided in various shapes, such as '-', ' ', '+' or ' '.
  • the cutout 122a of the pierceable blocking part may be provided as a completely cut line penetrating through the upper and lower surfaces or an incompletely cut line that cuts the upper surface but does not penetrate the lower surface.
  • the upper surface of the pierceable blocking part 122 is positioned lower than the upper end of the coupling member 110, and the lower surface of the pierceable blocking part 122 is positioned higher than the coupling member 110, it is possible to prevent contamination of the surface of the pierceable blocking part 122 when the liquid sample storage container is stored with the closure 100 open.
  • the blocking member 120 may include an inclined surface, which ascends radially outward, on the outer side of the connected portion of the side part 121 and the pierceable blocking part 122.
  • the pierceable blocking part 122 is elastically deformed downwardly and radially outward.
  • the blocking member 120 is formed with the inclined portion, the pierceable blocking part 122 may easily be deformed. Referring to FIG. 6, when the pipette 300 enters, the blocking member 120 is deformed so that the inclined portion comes closer to the inner circumferential surface of the main body part 210 of the storage container.
  • the blocking member 120 may be provided in various colors, to identify the type of the liquid sample contained in the chamber 220 of the storage container.
  • Polymer materials, such as rubber and elastomer, which may be applied as a material of the blocking member 120, are easy to dye and may allow an even color expression, rendering it easy to identify the type of liquid sample with the naked eye. If the type of the liquid sample is indicated by way of color, a separate sticker label may be unnecessary.
  • FIG. 8 is a view illustrating a liquid sample storage device with a closure 100-1 according to a second embodiment of the disclosure.
  • the closure 100-1 of the liquid sample storage container according to the second embodiment of the present invention may enhance the sealing degree in the cutout 122a of the pierceable blocking part by a modified shape of the blocking member 120.
  • a side part 121-1 of the blocking member may include a portion 121b having an outer diameter larger than an inner diameter of the upper opening of the main body part 210 of the storage container. Accordingly, as the side part 121-1 of the blocking member is inserted through the upper opening of the main body part 210, it is elastically deformed radially inward of the main body part 210 of the storage container, and elastic force F is exerted to the center of the pierceable blocking part 122, enhancing the sealability of the cutout 122a.
  • FIG. 9 is a view illustrating a liquid sample storage device with a closure 100-2 according to a third embodiment of the disclosure.
  • a sealing film 130 may be attached to the upper surface of the closure part 112 of the coupling member.
  • the sealing film may be provided to cover the opening 112a of the closure part 112 of the coupling member to prevent external contaminants from entering through the opening 112a of the closure part 112.
  • the sealing film may be detachably attached to the upper surface of the closure part 112 to be removable before the pipette 300 enters. Alternatively, the sealing film may be provided to be broken when the pipette 300 enters.
  • FIG. 10 is a view illustrating a liquid sample storage device with a closure 100-3 according to a fourth embodiment of the disclosure.
  • the closure 100-3 of the liquid sample storage container according to the fourth embodiment of the present invention may include the coupling member 110 and the blocking member 120. No duplicate description is given below of those described above.
  • the coupling member 110 may include a first body part 111 coupled to an upper portion of the main body part 210 of the storage container and a closure part 112 connected to an upper portion of the first body part 111.
  • the closure part 112 may form an opening 112a that extends inward from the upper portion of the first body part 111 and communicates with the chamber 220 of the storage container. When viewed from thereabove, the closure part 112 may be shaped as a circular disk or donut shape with the circular opening 112a.
  • the blocking member 120 may include a ring-shaped side part 121 interposed between the main body part 210 of the storage container and the second body part 113, a pierceable blocking part 122 connected to a lower portion of the side part 121 to block the upper opening of the chamber 220, and a seating part 123 radially bent outward of the main body part 210 of the storage container from an upper portion of the side part 121 and seated on an upper end of the main body part 210 of the storage container.
  • the blocking member 120 may further include a fourth fixing part 121b that is bent from the upper portion of the side part 121 radially outward of the main body part 210 of the storage container and extends and is coupled to the third fixing part 113b formed on the inner circumferential surface of the first body part 111 of the coupling member.
  • the third fixing part 113b may be provided as a groove that is recessed circumferentially from the inner circumferential surface of the first body part 111 of the coupling member
  • the fourth fixing part 121b may be provided as a ring-shaped protrusion fitted to the third fixing part 113b.
  • the fourth fixing part 121b and the seating part 123 may be integrally formed.
  • the seating part 123 may be a portion of the lower surface of the fourth fixing part 121b.
  • the blocking member 120 may be provided without the side part 121.
  • all of the pierceable blocking part 122, the seating part 123, and the fourth fixing part 121b may be provided as one configuration of the same plane or a plane adjacent thereto.
  • closure 100-3 of the liquid sample storage container according to the fourth embodiment of the disclosure omits the second body part 113 of the coupling member of FIG. 5, allowing the side part 121 of the blocking member to be reduced in length or height. In some cases, it is also possible to omit the side part 121 of the blocking member. Therefore, the closure 100-3 of the liquid sample storage container according to the fourth embodiment of the present invention may be manufactured to have a low height.
  • FIGS. 11 to 13 are cross-sectional views of closures 100-4, 100-5, and 100-6 of a liquid sample storage container according to embodiments of the disclosure.
  • the closures 100-4, 100-5, and 100-6 of the liquid sample storage container according to embodiments of the present invention have double pierceable blocking parts 122-1 and 122-2, preventing external scattering or evaporation of the excess liquid sample on the tip of the pipette 300 when the pipette 300 retracts after sucking the liquid sample.
  • the first pierceable blocking part 122-1 positioned relatively below may wipe off the excess liquid sample on the tip of the pipette 300 when the pipette 300 retracts after sucking the liquid sample
  • the second pierceable blocking part 122-2 positioned above and apart from the first pierceable blocking part 122-1 by a predetermined gap G may prevent external scattering or evaporation of the excess liquid sample wiped out by the first pierceable blocking part 122-1.
  • the first pierceable blocking part 122-1 may be relatively thicker than the second pierceable blocking part 122-2 and has a pre-cut cutout 122a to mor effectively wipe out the excess liquid sample on the tip of the pipette 300.
  • the second pierceable blocking part 122-2 is relatively thinner than the first pierceable blocking part 122-1 and is formed to have no cutout but allow entry of the pipette 300 to effectively block entry of external contaminants during storage or transport.
  • the first blocking member 120-1 including the first pierceable blocking part 122-1 may include a supporting surface 126 that supports the second blocking member 120-2 including the second pierceable blocking part 122-2.
  • the first blocking member 120-1 may include a ring-shaped side part 121-1 interposed between the main body part 210 of the storage container and the second body part 113 and the first pierceable blocking part 122-1 connected to a lower portion of the first side part 121-1, prepared to have elastic restorative force, and permitting entry of the pipette 300 while keeping the upper opening of the chamber 220 closed while not entered by the pipette 300.
  • the second blocking member 120-2 may include the second pierceable blocking part 122-2 that is disposed, in a center area of the main body part 210 of the storage container, above and the predetermined gap G apart from the first pierceable blocking part 122-1, prepared to have elastic restorative force, and permits entry of the pipette 300 while keeping the upper opening of the chamber 220 closed while not entered by the pipette 300.
  • the first side part 121-1 of the first blocking member 120-1 may form a step in a position spaced apart by the predetermined gap G from the upper surface of the first pierceable blocking part 122-1, and the second pierceable blocking part 122-2 may be seated on and supported by the step of the first side part 121-1.
  • a second fixing part 121a may be formed on the inner circumferential surface of the second side part 121-2 of the second blocking member 120-2 to be fixed to the first fixing part 113a by a hooking structure.
  • the second blocking member 120-2 including the second pierceable blocking part 122-2 may include a second seating part 123-2 seated on the first seating part 123-1 of the first blocking member 120-1.
  • the first blocking member 120-1 may include a ring-shaped first side part 121-1 interposed between the main body part 210 of the storage container and the second body part 113 and a first seating part 123-1 that is bent radially outward from the upper portion of the first side part 121-1 and seated on the upper portion of the main body part 210 of the storage container.
  • the second blocking member 120-2 may include a ring-shaped second side part 121-2 interposed between the main body part 210 of the storage container and the first side part 121-1 and a second seating part 123-2 that is bent radially outward from the upper portion of the second side part 121-2 and seated on the upper portion of the first seating part 123-1.
  • the second pierceable blocking part 122-2 may be positioned the predetermined gap G apart from the first pierceable blocking part 122-1, with the second seating part 123-2 seated on the first seating part 123-1.
  • a second fixing part 121a may be formed on the inner circumferential surface of the second side part 121-2 of the second blocking member 120-2 to be fixed to the first fixing part 113a by a hooking structure.
  • the first blocking member 120-1 including the first pierceable blocking part 122-1 may include a supporting surface that supports the second blocking member 120-2 including the second pierceable blocking part 122-2.
  • the first blocking member 120-1 may include a ring-shaped side part 121-1 interposed between the main body part 210 of the storage container and the second body part 113 and the first pierceable blocking part 122-1 connected to a lower portion of the first side part 121-1, prepared to have elastic restorative force, and permitting entry of the pipette 300 while keeping the upper opening of the chamber 220 closed while not entered by the pipette 300.
  • the second blocking member 120-2 may include the second pierceable blocking part 122-2 that is disposed, in a center area of the main body part 210 of the storage container, above and the predetermined gap G apart from the first pierceable blocking part 122-1, prepared to have elastic restorative force, and permits entry of the pipette 300 while keeping the upper opening of the chamber 220 closed while not entered by the pipette 300.
  • the first pierceable blocking part 122-1 of the first blocking member 120-1 may include a first surface 127 supporting the second pierceable blocking part 122-2 and a second surface 128 prepared as a groove recessed from the first surface 127 and positioned lower than the first surface 127.
  • the second surface 128 may be prepared as an upper surface of the groove recessed to a predetermined depth G around the center area of the first pierceable blocking part 122-1 and a cutout 122a may be formed in the second surface 128.
  • the second pierceable blocking part 122-2 may be seated and supported on the first surface 127 of the first pierceable blocking part 122-1. In this case, the second pierceable blocking part 122-2 may be disposed the predetermined gap G apart from the cutout 122a of the first pierceable blocking part 122-1.
  • a second fixing part 121a may be formed on the inner circumferential surface of the first side part 121-1 of the first blocking member 120-1 to be fixed to the first fixing part 113a by a hooking structure.

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  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

Selon un mode de réalisation, une fermeture d'un récipient de stockage d'échantillon liquide, fixée à une ouverture supérieure d'un récipient de stockage ayant une chambre permettant de stocker un échantillon liquide, peut comprendre un élément d'accouplement accouplé à une partie supérieure du récipient de stockage et un élément de blocage bloquant l'ouverture supérieure du récipient de stockage. L'élément d'accouplement peut comprendre une première partie corps, une seconde partie corps et une partie fermeture. L'élément de blocage peut comprendre une partie latérale interposée entre le récipient de stockage et la seconde partie corps, et une partie de blocage pouvant être percée reliée à une partie inférieure de la partie latérale, préparée pour présenter une force de restauration élastique, et permettant l'entrée d'une pipette tout en maintenant l'ouverture supérieure de la chambre fermée tant que la pipette n'y entre pas. Une première partie de fixation peut être formée pour fixer la partie latérale de l'élément de blocage.
PCT/KR2022/004479 2021-03-31 2022-03-30 Fermeture d'un récipient de stockage d'échantillon liquide et dispositif de stockage d'échantillon liquide la comprenant WO2022211475A1 (fr)

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KR20210041551 2021-03-31
KR10-2021-0041551 2021-03-31

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5297599A (en) * 1991-03-19 1994-03-29 Hoffmann-Laroche Inc. Closure device for sealing reagent containers in an automatic pipetting system
US5361921A (en) * 1993-06-29 1994-11-08 Becton Dickinson And Company Combination stopper-shield closure
WO2000069389A2 (fr) * 1999-05-14 2000-11-23 Gen-Probe Incorporated Capuchon pouvant etre perce et appareil de transfert de liquide utilisable avec ledit capuchon
EP1082998A2 (fr) * 1999-07-09 2001-03-14 Becton Dickinson and Company Dispositif de prélèvement d'échantillons avec capuchon
US20140011292A1 (en) * 2007-04-16 2014-01-09 Becton, Dickinson And Company Pierceable cap
CN110553877A (zh) * 2018-05-31 2019-12-10 深圳迈瑞生物医疗电子股份有限公司 用于自动进样的微量样本采集管

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5297599A (en) * 1991-03-19 1994-03-29 Hoffmann-Laroche Inc. Closure device for sealing reagent containers in an automatic pipetting system
US5361921A (en) * 1993-06-29 1994-11-08 Becton Dickinson And Company Combination stopper-shield closure
WO2000069389A2 (fr) * 1999-05-14 2000-11-23 Gen-Probe Incorporated Capuchon pouvant etre perce et appareil de transfert de liquide utilisable avec ledit capuchon
EP1082998A2 (fr) * 1999-07-09 2001-03-14 Becton Dickinson and Company Dispositif de prélèvement d'échantillons avec capuchon
US20140011292A1 (en) * 2007-04-16 2014-01-09 Becton, Dickinson And Company Pierceable cap
CN110553877A (zh) * 2018-05-31 2019-12-10 深圳迈瑞生物医疗电子股份有限公司 用于自动进样的微量样本采集管

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