WO2022180231A1

WO2022180231A1 - Composition comprising an extract of vitis vinifera, vitamin c and probiotics for combating ageing of the skin

Info

Publication number: WO2022180231A1
Application number: PCT/EP2022/054838
Authority: WO
Inventors: David Gaudout; Stéphane REY; Benoit Lemaire; Séverine DUBREUIL
Original assignee: Activ'inside
Priority date: 2021-02-26
Filing date: 2022-02-25
Publication date: 2022-09-01
Also published as: FR3120188B3; FR3120188A3

Abstract

The present invention relates to a composition comprising at least one extract of Vitis vinifera, vitamin C, and at least one probiotic bacterium and/or probiotic yeast. Such a composition is intended to be used for preventing and/or combating ageing of the skin in humans or animals.

Description

COMPOSITION COMPRISING AN EXTRACT OF VITIS VINIFERA, VITAMIN C AND PROBIOTICS TO FIGHT AGAINST SKIN AGING

[0001] Technical area

The invention relates to a composition comprising at least one extract of Vitis vinifera, vitamin C, and at least one probiotic bacterium and/or probiotic yeast. Such a composition is intended to be used for preventing and/or combating skin aging in humans or animals.

[0003] Prior art

[0004] Oxidative stress is caused by an imbalance between reactive species, also called “reactive oxygen species” (ROS), originating from the metabolism of oxygen and nitrogen, and antioxidants.

[0005] Free radicals are constantly produced by the organism in the cells, in particular at the level of the mitochondria. Many biological processes such as respiration, inflammation, hormone intervention or synthesis, programmed cell death, but also exogenous factors such as pollution, physiological stress, physical inactivity, smoking or UV exposure may be involved in the production of free radicals.

[0006] Over time, oxidative stress is visibly reflected, at the skin level, by a loss of elasticity and firmness, but also by pigmentation disorders. Also, oxidative stress promotes the appearance of fine lines and wrinkles and therefore skin aging. Oxidative stress can also alter the physiological function of the skin, ie its role as a “barrier” which protects the body in particular against pathogens but also against water loss. Consequently, oxidative stress inevitably leads to a decrease in skin hydration, reinforces the fragility of the barrier vis-à-vis the external environment and thus promotes skin ageing. The contribution of antioxidants makes it possible to reduce these deleterious effects on the appearance and function of the skin. Patent FR 3049858 thus describes a cosmetic composition comprising such antioxidants for combating skin aging.

[0007] On the other hand, the high production of free radicals can also be linked to the inflammatory process. Indeed, the activation of phagocytes induces the activation of NADPH oxidase, which catalyzes the production of a large quantity of reactive species (superoxide radical). Conversely, excessive oxidative stress leads to an increase in the production of pro-inflammatory molecules. For this reason, oxidative stress is implicated in the etiology of certain inflammatory pathologies.

[0008] The immune system also produces reactive species through the activation of neutrophils which then produce free radicals in significant quantities. However, immune cells are particularly sensitive to oxidative stress. Also, an increase in oxidative stress is often associated with a decrease in immune functions.

[0009] Vitamin C is known to play a major role both in skin homeostasis, in the body's defenses against oxidative damage, and in immune functions.

[0010] Polyphenols, in particular grape polyphenols, are well known for their powerful antioxidant power, both orally and topically. Several preclinical and clinical studies show beneficial effects on skin aging, in particular on the homogeneity of the complexion as well as on the protection of skin cells against damage induced by UV exposure.

[0011] Probiotics are well known for their beneficial effects on the immune functions, in particular at the digestive level, but also at the level of the otorhinolaryngeal (ENT) sphere. More recently, studies have shown that certain probiotic strains could also play a role against oxidative stress and against pro-inflammatory processes.

[0012] The beneficial effects of vitamin C, polyphenols and probiotics taken individually are thus known in preventing and combating skin aging in humans or animals.

[0013] However, skin aging remains a major concern for a large number of people. Also, there is still a need for a new solution making it possible to prevent and/or fight against skin aging in humans or animals with improved efficiency compared to the prior art. This is the objective of the present invention.

[0014] Summary of the Invention To meet this need, the present invention provides a new composition comprising at least vitamin C, at least one extract of Vitis vinifero and at least one probiotic bacterium and/or probiotic yeast.

[0016] Such a composition is not known from the prior art. However, the combination of an extract of Vitis vinifero comprising polyphenols, vitamin C and at least one probiotic bacterium and/or probiotic yeast is particularly advantageous. Such an association makes it possible to obtain a synergistic effect for combating skin aging compared to the extract, the vitamin C or the probiotic consumed individually by humans or animals.

[0017] On the other hand, polyphenols are well known for having antimicrobial activity. However, unexpectedly, the combination of polyphenols provided by the extract of Vitis vinifero does not exhibit such deleterious effects on the probiotics of the invention.

To this end, the present invention provides a new composition comprising: at least vitamin C, at least one extract of Vitis vinifero, and at least one probiotic bacterium and/or probiotic yeast.

[0019] The extract of Vitis vinifera preferably comprises a mixture of molecules comprising at least 50% of total polyphenols measured by spectrophotometry at OD280nm, the percentage being given by dry weight relative to the total dry weight of the extract of Vitis vinifera, said total polyphenols comprising:

^■ at least 15% of flavanol monomers measured by HPLC, the percentage being given by dry weight relative to the total dry weight of the Vitis vinifera extract,

^■ at least 5% of flavanol oligomers measured by HPLC, the percentage being given by dry weight relative to the total dry weight of the Vitis vinifera extract.

[0020] The probiotic bacterium and/or probiotic yeast integrated into the present composition is preferably chosen from bacteria and/or yeasts of the genus Lactobacillus, Bifidobocterium, Streptococcus, Socchoromyces, Enterococcus, Pediococcus, Escherichia and mixtures thereof.

The composition according to the invention may also comprise superoxide dismutase (SOD), preferably from melon.

[0022] Said composition may be in the form of a nutritional composition, a cosmetic composition or a food supplement for preventing and/or combating normal skin aging in a healthy subject or the symptoms of “razor burn” or against damage resulting from exposure to pollution.

[0023] Thus, the composition is intended to be used for an anti-aging and/or moisturizing cosmetic effect and/or to fight against "razor burn" and/or to prevent and/or fight against skin aging. .

[0024] It may also be in the form of a medicament or a pharmaceutical composition for preventing and/or treating skin pathologies chosen from acne and/or dermatitis and/or erythema and/or eczema. and/or rosacea.

[0025] According to another object, the invention relates to a cosmetic use of the composition as well as a cosmetic treatment process.

Other characteristics and advantages will emerge from the detailed description of the invention and the examples which follow.

[0027] Detailed description of the invention [0028] Definition

[0029] By "nutritional composition" is meant within the meaning of the invention any mixture of food compounds intended to be ingested by a healthy subject and comprising vitamin C, an extract of Vitis vinifera and at least one probiotic bacterium and/or or probiotic yeast. The nutritional compositions are in particular food compositions, foods or drinks, but can also be food supplements.

[0030] By "pharmaceutical composition" or "drug" is meant in the sense of the invention any composition comprising at least one active substance presented as having curative or preventive properties with regard to human or animal diseases used alone or associated to other active or non-active substances and intended to be ingested by a pathological subject. Such a composition comprising vitamin C, an extract of Vitis vinifero and at least one probiotic bacterium and/or probiotic yeast. Said pharmaceutical composition or medicinal product is intended for humans or animals in the prevention and/or treatment of skin pathologies such as acne and/or dermatitis and/or erythema and/or eczema and /or rosacea.

[0031] By "healthy subject" is meant in the sense of the invention a subject who may be a human or an animal not suffering from any skin pathology such as acne and / or dermatitis and / or erythema and/or eczema and/or rosacea. [0032] Conversely, the term “pathological subject” is understood to mean, within the meaning of the invention, a subject who suffers from at least one skin pathology such as acne and/or dermatitis and/or erythema and /or eczema and/or rosacea.

[0033] By “an extract of Vitis vinifero”, is meant a set of molecules obtained from an extract of Vitis vinifera. The raw material of Vitis vinifero can be the leaves and/or the fruits and/or the pips and/or the woods, preferentially the raw material of Vitis vinifero is the aerial part of the plant, that is to say the all the leaves, fruits, skin (skin), pips and wood, more preferably the skin (skin) and the pips.

[0034] By “at least 15% of flavanol monomers” is meant either at least 15% of catechins and/or epicatechins if there are no other flavanol monomers in the mixture, or at least 15% of catechins if there are no epicatechins in the mixture, or at least 15% of epicatechins if there are no catechins in the mixture, or at least 15% of the mixture of catechins and d epicatechins if both catechins and epicatechins are present in the mixture. Preferably, at least 15% of the mixture of catechins and epicatechins is meant.

By “ppm” is meant parts per million (mg/kg) in the mixture. Unless otherwise stated, ppm refers to a weight based on the total weight of the mixture.

[0036] By “SOD from vine”, or “SOD from Vitis vinifero” is meant, within the meaning of the invention, SOD obtained from a vine leaf extract, preferably from Vitis vinifera. The extract of Vitis vinifero providing SOD is different from the extract comprising at least 50% of total polyphenols as described above in the sense that said extract rich in polyphenols contains little (in trace form) or no SOD. The extract comprising SOD can in particular be obtained according to the method described in patent FR3003164. [0037] By "the bacterium and/or probiotic yeast" is meant in the sense of the invention a bacterium or bacteria of the same genus or species but also a mixture of bacteria of different genera or of different species. The probiotic bacteria and/or yeast is therefore also understood as the probiotic bacteria and/or yeasts.

The invention is now illustrated by non-limiting examples of compositions according to the invention and by results.

[0039] Composition

[0040] The object of the invention is therefore to provide a composition having improved efficacy in preventing and/or combating skin aging and thus having an anti-aging and/or moisturizing cosmetic effect.

To this end, the subject of the present invention is therefore a composition comprising: at least vitamin C, at least one extract of Vitis vinifera, and at least one probiotic bacterium and/or probiotic yeast.

[0042] Vitamin C (ascorbic acid) has characteristics suitable for a cosmetic, nutritional, food application, as a food supplement or as a medicine. It can come from a natural product, in particular fruits and vegetables such as guavas, kiwis, oranges, strawberries, broccoli, grapefruit, acerola or from a synthetic product.

Preferably, the vitamin C is present in the composition in an amount of at least 1 mg/g of dry matter of the total weight of the composition. Thus, the amount of vitamin C present in the composition is preferably equivalent to an administration amount of at least 4 mg per day, ie at least 5% of the Nutritional Reference Value (NRV).

The extract of Vitis vinifera present in the present composition preferably comprises a mixture of molecules comprising at least 50% of total polyphenols measured by spectrophotometry at an OD280nm, the percentage being given by dry weight relative to the total dry weight of the extract of Vitis vinifera, said total polyphenols comprising: at least 15% of flavanol monomers, preferably comprising catechins and epicatechins, measured by HPLC, the percentage being given by dry weight relative to the total dry weight of the extract of Vitis vinifera, at least 5% of flavanol oligomers measured by HPLC, the percentage being given by dry weight relative to the total dry weight of the Vitis vinifera extract. Catechins and epicatechins are compounds of the flavonoid family, in particular of the flavanol subclass, preferably flavanol monomers. They are also known as catechol and epicatechol and are powerful antioxidants.

Preferably, the Vitis vinifera extract comprises a mixture of molecules also comprising stilbenes such as resveratrol and/or its oligomers or polymers such as epsilon viniferine, in particular said mixture comprises at least 15 ppm of stilbenes, the weight being given by dry weight relative to the total dry weight of the extract of Vitis vinifera.

Resveratrol is particularly known to be present in grapes. However, it can also be derived from other plant sources, in particular from Polygonum cuspidatum but also by biotechnological means. It has many beneficial activities for humans or animals including its antioxidant effects to fight against skin aging.

The effect of the composition is particularly increased and effective when it also comprises:

- at least 50 ppm of quercetins and/or quercetin glycosides and/or

- at least 500 ppm of anthocyanins.

[0049] Quercetin and the consumption of foods rich in anthocyanidins (aglycones and/or glycosylated) helps to fight against cellular ageing.

The Vitis vinifera extract comprising at least 50% of total polyphenols can be obtained from an extract of grape marc and/or grape seed, more preferably the Vitis vinifera extract is obtained from of a grape seed extract and an extract of grape marc.

The extract of Vitis vinifera can also comprise stilbenes (resveratrol and/or its oligomers or polymers in particular epsilon viniferine) with a concentration of at least 15 ppm. To achieve this, the extract of Vitis vinifera can be obtained from a raw material that includes skins, stalks or stems with or without grape seeds. A particularly suitable method for obtaining an extract of Vitis vinifera (fruit, skin, pip, leaf, stalk, vine shoot, stock, wood, root) comprises the following steps: a. extraction with water and/or alcohol (in particular ethanol) of Vitis vinifera, preferentially pips of Vitis vinifera on the one hand, and on the other hand grape marc of Vitis vinifera (dandruff/skin, pips, stalks) resulting in particular from pressing residues from the production of wine and/or leaves of Vitis vinifera and/or vine shoots, vines, wood, root of Vitis vinifera. The amount of water or hydroalcoholic solution (30% v/v to 96% V/V) added is between 2 and 10 times the mass of raw material used. The duration of the extraction can be between 30 minutes and 24 hours and the extraction temperature between 20°C and 80°C. The Raw materials used can be in dry, fresh, or frozen whole or ground form. b. separation of the water and/or alcohol solution from the solid material, for example by centrifugal decantation or by pressing and filtration. The extract thus obtained is called crude extract A. c. According to a variant, the method may optionally comprise removal of the alcohol by evaporation under vacuum at a temperature, preferably below 60° C. and at a pressure below 100 mbars. d. from the crude extract A previously obtained, a specific stage of the process may make it possible to obtain a purified extract rich in flavanol monomers either: i. by purification by membrane separation of ultrafiltration and/or nanofiltration known to those skilled in the art of the extract desolventized beforehand so as to preferentially select the monomers and to eliminate the flavanol polymers, making it possible to obtain an extract comprising a fraction enriched in flavanol monomers; or ii. by purification using a liquid/liquid separation using organic solvents known to those skilled in the art and in particular using ethyl acetate, or iii. by purification via precipitation with salts such as NaCl of the flavanol polymers followed by a solid/liquid separation such as filtration for example. e. The extract thus obtained is a purified extract rich in flavanol monomers called extract B. f. An additional step may allow chromatographic purification of extract A and/or extract B. This step will consist of passing over a chromatographic column containing adsorption resins particularly suitable for polyphenols and known to those skilled in the art. art, extracts A and/or B then rinsing the resins using an aqueous solution followed by an elution phase using a hydroethanolic solution preferably between 60 and 80% v /vg The alcohol of the eluates thus obtained will then be evaporated and the eluate concentrated via a vacuum concentration step of the eluates rich in polyphenols and more particularly in flavanol monomers and/or in e-viniferin at a temperature preferably below 60° C. and a pressure below 100 mbar; h. An additional step will consist in drying the purified extracts by techniques known to those skilled in the art such as atomization, drying in a vacuum oven or drying by freeze-drying with or without a support such as maltodextrin, gum arabic for example. i. Once the extract has been obtained, the process for preparing the composition may comprise the mixture of extracts of Vitis vinifero rich on the one hand in flavanol monomers and on the other hand in e-viniferin. d. A mixture of Vitis vinifera extract rich in polyphenol obtained according to the preceding steps can be combined with an extract rich in SOD which can be obtained according to a method as described in patent FR3003164.

Finally, the composition according to the invention also comprises at least one probiotic bacterium and/or probiotic yeast which, combined with the extract of Vitis vinifera and with vitamin C, makes it possible to substantially increase the anti-aging efficacy. Also, surprisingly, the specific combination as well as the quantity of the molecules found in the present composition makes it possible to obtain an effect on skin aging in humans or animals, this effect being better than that of constituents of the present invention taken alone or cosmetic compositions of the prior art. Said probiotic bacterium and/or probiotic yeast integrated into the present composition is preferably chosen from bacteria and/or yeasts of the genus Lactobacillus, Bifidobacterium, Streptococcus, Saccharomyces, Enterococcus, Pediococcus, Escherichia and mixtures thereof.

Preferably, the probiotic bacterium and/or yeast is chosen from the species Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus acidophilus, Lactobacillus johnsonii, Lactobacillus delbrueckii ssp bulgaricus, Lactobacillus casei, Lactobacillus salivarius, Lactobacillus paracasei, Lactobacillus rhamnosus, Bifidobacterium bifidum, Bifidobacterium animaiis, Bifidobacterium infantis, Bifidobacterium lactis, Bifidobacterium longum, Bifidobacterium animaiis, Bifidobacterium bifidum, Bifidobacterium breve, Streptococcus thermophiles, Sacaromyces cerevisiae, Streptococcus faecium, Enterococcus Faecalis, Escherichia coli and mixtures thereof.

[0057] Preferably, the probiotic bacteria and/or probiotic yeast is dead, inactivated, semi-inactivated or alive, in the form of spores or not.

According to an advantageous embodiment, the composition daily provides between 1×10 ⁵ and 9×10 ¹² CFU, preferably between 1×10 ⁷ and 9× ^{10 11} of a probiotic bacterium and/or yeast and/or of a mixture.

According to a particularly preferred embodiment, the composition according to the invention also comprises superoxide dismutase (SOD).

The SOD is preferably an SOD derived from melon (Cucumis melo), vine (Vitis vinifera) or olive tree (Olea europeoe), because it has very good antioxidant properties and characteristics suitable for cosmetic application. More preferably, the SOD is an SOD derived from melon (Cucumis melo).

The composition therefore preferably comprises a melon, vine or olive tree extract containing SOD. When the extract is from a vine, it may be an extract of Vitis vinifero but said extract is not an extract of Vitis vinifera comprising at least 50% of total polyphenols including at least 15% of catechins and/or epicatechins and/ or at least 5% flavanol oligomers. Said extract providing SOD can be obtained by a process for obtaining SOD or the extract containing SOD by aqueous extraction, without organic solvent. SOD is indeed a particularly fragile molecule which requires mild extraction conditions. The SOD may also optionally be a synthetic SOD.

Preferably, the vine extract, preferably an extract of Vitis vinifera is encapsulated or microencapsulated. Advantageously, said extract is encapsulated or microencapsulated in a food carrier chosen from maltodextrin, gum arabic, hydrogenated or non-hydrogenated oil, wax, alginates, starch, hydrolyzed or non-hydrolyzed proteins, phosphatidylcholine, phospholipids, galactomannan, emulsifiers chosen from pectin or polyglycerol polyricinoleate and fibers chosen from polydextrose, HPMC and cellulose. Encapsulation is particularly advantageous and makes it possible to stabilize and protect the extract.

By extract is meant a molecule or a mixture of molecules obtained from these plants. The extracts containing SOD incorporated into the compositions according to the invention may consist exclusively of SOD or else of SOD and one or more other molecules such as polyphenols with an SOD content of at least 100 IU/g of material. dry matter of the extract and even more preferably at least 500 IU/g of dry matter of the extract and even more preferably at least 1000 111/g of dry matter of the extract. The SOD (or the extract containing SOD) used according to the invention is preferably associated with a suitable vehicle for the intended use: external use for a cosmetic composition or a medicament for topical administration; ingestion and assimilation by the body for a food composition or a food supplement or a medicament for oral administration. In particular, the SOD is microencapsulated beforehand within polymeric biodegradable microparticles or lipid substances.

When the composition comprises superoxide dismutase (SOD), the SOD/Vitamin C ratio in the composition is preferably between 1 and 50 and the SOD is present in the composition in an amount of at least 100 IU/g. of dry matter of the total weight of the composition, preferably at least 500 IU/g of dry matter of the total weight of the composition.

[0066] The composition according to the invention, in addition to SOD, vitamin C, Vitis vinifera extract and probiotics, may contain other active molecules that are effective in combating skin aging, such as zinc and/or at least one other vitamin chosen from vitamins B1, B2, B3, B3/PP, B5, B6, B8/H, B9, B12, E and D.

Said vitamins may be derived from natural products, in particular fruits and vegetables or synthetic products.

The composition may also comprise at least one prebiotic chosen from inulin, oligosaccharides, FOS (fructo-oligosaccharides), GOS (galacto-oligosaccharides), CLA (conjugated fatty linoleic acid), PUFA (polyunsaturated acid) , MOS (mannanoligosaccharide) and XOS (xylooligosaccharide).

[0069] The addition of a prebiotic makes it possible to reinforce the effectiveness of the composition. When they are associated, mixed, combined with an extract of Vitis vinifera, at least one probiotic and vitamin C, the composition exhibits an improved effect on skin aging.

The composition according to the invention can be administered orally in the form of a food supplement, a nutritional product or a medicine or applied topically to the skin in the form of a cosmetic product or a medicine suitable for topical application. Also, the composition also comprises excipients suitable in depending on its mode of administration.

For oral administration, it may be in particular maltodextrin, starch, gum arabic, vegetable oil, sorbitol or water. The composition is then in the form of a powder, an oil or a liquid solution.

For topical administration to the skin, it may be in particular water, vegetable, mineral or silicone oils, vegetable fats. The composition is then in the form of a cream, ointment, emulsion, gel or lotion.

Preferably, the composition is encapsulated or microencapsulated, preferably the composition is encapsulated or microencapsulated in a food carrier chosen from maltodextrin, gum arabic, hydrogenated or non-hydrogenated oil, wax, alginates, starch, hydrolyzed or non-hydrolyzed proteins, phosphatidylcholine, phospholipids, galactomannan, emulsifiers chosen from pectin or polyglycerol polyricinoleate and fibers chosen from polydextrose, HPMC and cellulose.

Encapsulation is particularly advantageous and makes it possible to stabilize and protect the Vitis vinifera extract and/or the probiotic(s) (bacteria and/or yeasts) from its environment, in particular from the organism.

In addition, encapsulation makes it possible to improve the stability, the bioavailability and the use of said extract in food matrices, with in particular masking of the taste and resistance to possible alterations of the compounds during the stages of production of a food product.

Microencapsulation provides the same advantages as encapsulation but more particularly makes it possible to insert active substances into particles of size between 1 μm and 1000 μm and more preferentially between 30 μm and 500 μm and even more preferentially between 50pm and 300pm.

The composition according to the invention is intended to be administered as a nutritive product and can therefore be in different forms, preferably in the form of powder, capsule, tablet, capsule, solution, suspension , emulsion or chewing gum.

When the composition is intended to be administered as a nutritional product, it can also be in the form of a food product, preferably in the form of dairy products, purees, cereals, cereal products and drinks.

When the composition is administered as a therapeutic product, the invention relates to a composition for its use as a medicament for humans or animals and can be in different forms, preferably in the form of powder, capsule, tablet, effervescent tablet, capsule, solution, suspension.

When the composition is in drug form, the composition according to the invention is preferably intended for use in the prevention and/or treatment of skin pathologies chosen from acne and/or dermatitis and/or erythema and/or eczema and/or rosacea.

[0081] The composition also exhibits a cutaneous anti-aging cosmetic effect in a healthy subject which is characterized in particular by the reduction of cutaneous imperfections (spots, redness, dark circles, etc.), the maintenance or restoration of firmness and the elasticity of the skin, the improvement of the complexion of the skin (colors, luminosity, contrast, shine, etc.), the maintenance or restoration of good hydration of the skin.

The composition can be obtained by the process comprising the following steps. [0083] In a mixer, for example of the ribbon, V, plowshare type (not exhaustive), the compounds in dry form will be mixed. Optionally, a premix with the extract containing SOD alone or with vitamin C, added to a quantity of excipient not exceeding 80% by weight of the premix may be manufactured before being itself mixed with the total excipients and other constituents of the formula. In order to obtain a homogeneous mixture, the mixing operation may last between 5 minutes and 30 minutes. According to a variant, the method may consist in directly adding vitamin C with the other constituents of the formula with stirring for 5 to 30 minutes at a temperature below 40° C., preferably and optionally under inert gas.

For compositions in liquid, pasty or gelled forms, it is appropriate, with stirring, to finely disperse the vitamin C in the hydrophilic phase. When the composition comprises SOD, the extract containing the SOD may itself be finely dispersed in the lipophilic phase containing an emulsifier. The temperature of the lipophilic phase will preferably not exceed 40°C. The 2 phases can then be mixed between 5 minutes and 30 minutes until a homogeneous mixture is obtained.

[0086] For the dry forms, the product, once mixed, will be packaged in the galenic adapted to the finished product (capsule, tablet, stick) according to conventional techniques known to those skilled in the art.

For liquid forms, the mixture may be homogenized using a homogenizer and may also possibly undergo a pasteurization or sterilization step before packaging according to known and conventional techniques. of career.

[0088] Use and method of cosmetic treatment

The invention also relates to a method for the cosmetic treatment of the skin, to prevent and/or combat the visible manifestations of normal aging of the skin, which consists in applying to the skin, in particular to the skin of the face, at least once a day, a cosmetic composition according to the invention, suitable for topical administration to the skin. Twice daily application is preferred.

The invention also relates to a cosmetic treatment process for preventing and/or combating the visible manifestations of normal skin aging, which consists in ingesting, at least once a day, a nutritional composition or a cosmetic composition according to the invention suitable for oral administration, in particular a food supplement with a cosmetic effect.

According to another object, the invention is aimed at a cosmetic use of the composition according to the invention, for an anti-aging and/or moisturizing cosmetic effect and/or for combating razor burn.

Preferably, the cosmetic use of the present composition aims to obtain a cosmetic effect allowing:

- to reduce skin imperfections such as spots, redness and/or dark circles, and/or

- to maintain and/or restore the firmness and elasticity of the skin and/or

- to improve the complexion of the skin and/or to maintain and/or improve the hydration of the skin.

[0093] Examples

[0094] Example 1: Nutritional composition intended for humans [0095] Example 1 is a 381 mg capsule, consisting of:

An extract of Vitis vinifero: 250 mg Vitamin C: 6 mg Blend of 6 probiotics (Lactobacillus fermentum, L plantarum, L acidophilus, Bfidobacterium lactis, B. longum, B. bifidum) 3c10 ^L 9 CFU: 75 mg Maltodextrin: 50 mg.

The composition is obtained by mixing the constituents under the conventional conditions known to those skilled in the art, and placed in a capsule according to the conventional conditions as well. The recommended dosage is 2 capsules per day.

[0096] Example 2: Nutritional composition intended for humans [0097] Example 2 is a 3200 mg sachet, consisting of:

An extract of Vitis vinifero: 600 mg Vitamin C: 12 mg SOD: 100 mg

Blend of 3 probiotics (Lactobacillus acidophilus, Bifidobacterium bifidum,

Lactobacillus fermentum) 4c10 ^L 9 CFU: 2300 mg

Zinc: 3mg

Vitamin B6: 0.5mg

Maltodextrin: 184.5 mg.

The composition is obtained by mixing the constituents under conventional conditions known to those skilled in the art and is in the form of a powder contained in a sachet also under conventional conditions. The recommended dosage is 1 sachet per day.

[0098] Example 3: Nutritional composition intended for animals [0099] Example 3 is a soft capsule consisting of:

An extract of Vitis vinifera: 50 mg Vitamin C: 24 mg SOD: 50 mg

Mixture of 2 probiotics (Enterocossus faecium, Lactobacillus acidophilus casei) 3c10 ^L 9 CFU: 21 mg - FOS: 100 mg

Evening primrose oil: 400 mg.

The composition is obtained by mixing the constituents under the standard conditions known to those skilled in the art and is in the form of a soft capsule also under the standard conditions. The recommended dosage is 1 capsule per day. Example 4: Nutritional composition intended for the animal

Example 4 is a tablet consisting of:

An extract of Vitis vinifero: 50 mg Vitamin C: 5 mg - SOD: 50 mg

Blend of 5 probiotics (Lactobacillus acidophilus, L. plantarum, L Brevis, Bifidobacterium animalis lactis, Lactobacillus ramnosus) 2c10 ^L 9 CFU: 26.5 mg - FOS: 250 mg.

The composition is obtained by mixing the constituents under the standard conditions known to those skilled in the art and is in the form of a tablet also under the standard conditions. The recommended dosage is 1 tablet per day for a 10 kg dog.

Claims

[Claim 1] Composition comprising: at least vitamin C, at least one extract of Vitis vinifera, and at least one probiotic bacterium and/or probiotic yeast.

[Claim 2] Composition according to the preceding claim, characterized in that the extract of Vitis vinifero comprises a mixture of molecules comprising at least 50% of total polyphenols measured by spectrophotometry at OD280nm, the percentage being given by dry weight relative to the total dry weight of the extract of Vitis vinifera, said total polyphenols comprising: at least 15% of flavanol monomers measured by HPLC, the percentage being given by dry weight relative to the total dry weight of the extract of Vitis vinifera, at least 5% of flavanol oligomers measured by HPLC, the percentage being given by dry weight relative to the total dry weight of the extract of Vitis vinifera.

[Claim 3] Composition according to the preceding claim, characterized in that the mixture of molecules also comprises at least 15 ppm of stilbenes, the weight being given in dry weight relative to the total dry weight of the extract of Vitis vinifera.

[Claim 4] Composition according to the preceding claim, comprising at least superoxide dismutase (SOD), characterized in that the SOD/Vitamin C ratio in the composition is between 1 and 50 and in that the SOD is present in the composition in an amount of at least 100 µl/g of dry matter of the total weight of the composition.

[Claim 5] Composition according to the preceding claim, characterized in that the SOD is derived from vines, or melon or olive trees.

[Claim 6] Composition according to one of the preceding claims, characterized in that the vitamin C is present in the composition in an amount of at least 1 mg/g of dry matter of the total weight of the composition.

[Claim 7] Composition according to one of the preceding claims, characterized in that the probiotic bacterium and/or probiotic yeast is dead, inactivated, semi-inactivated or alive, in the form of spores or not.

[Claim 8] Composition according to one of the preceding claims, characterized in that the probiotic bacterium(ies) or yeast(s) is chosen from the genera Lactobacillus, Bifidobacterium, Streptococcus, Saccharomyces, Enterococcus, Pediococcus, Escherichia and their mixtures.

[Claim 9] Composition according to the preceding claim, characterized in that the probiotic bacterium(ies) or yeast(s) is chosen from the species Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus acidophilus, Lactobacillus johnsonii, Lactobacillus delbrueckii ssp bulgaricus, Lactobacillus casei, Lactobacillus salivarius, Lactobacillus paracasei, Lactobacillus rhamnosus; Bifidobacterium bifidum, Bifidobacterium animalis, Bifidobacterium infantis, Bifidobacterium lactis, Bifidobacterium longum, Bifidobacterium animalis, Bifidobacterium bifidum,

Bifidobacterium breve, Streptococcus thermophiles, Sacaromyces cerevisiae, Streptococcus faecium, Enterococcus Faecalis, Escherichia coli and their mixtures.

[Claim 10] Composition according to one of the preceding claims, characterized in that the composition provides between 1x10 ⁵ and 9x10 ¹² CFU daily, preferably between 1x10 ⁷ and 9x10 ¹¹ of a probiotic bacterium and/or yeast and/or a mixed.

[Claim 11] Composition according to one of the preceding claims, characterized in that it also comprises at least zinc and/or at least one other vitamin chosen from vitamins B1, B2, B3, B3/PP, B5, B6 , B8/H, B9, B12, E and D.

[Claim 12] Composition according to one of the preceding claims, characterized in that it also comprises at least one prebiotic.

[Claim IB] Composition according to the preceding claim, characterized in that the prebiotics are chosen from FOS, GOS and polyphenols derived from red fruits.

[Claim 14] Composition according to one of the preceding claims for its use in the prevention and/or treatment of acne and/or dermatitis and/or erythema and/or eczema and/or rosacea.

[Claim 15] Composition according to one of Claims 1 to 14, characterized in that the composition is a food supplement in the form of a capsule, powder, tablet, solution or chewing gum.

[Claim 16] Composition according to one of Claims 1 to 14, characterized in that the composition is a food product in the form of dairy products, cereals, cereal products or beverages.

[Claim 17] Composition according to one of Claims 1 to 14, characterized in that the composition is a cosmetic composition in the form of a cream, lotion, gel, mousse, ointment, poultice or patch.