WO2022138254A1 - Casier de stockage de lames de verre, dispositif de stockage de lames et système d'imagerie de lames en verre - Google Patents

Casier de stockage de lames de verre, dispositif de stockage de lames et système d'imagerie de lames en verre Download PDF

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Publication number
WO2022138254A1
WO2022138254A1 PCT/JP2021/045714 JP2021045714W WO2022138254A1 WO 2022138254 A1 WO2022138254 A1 WO 2022138254A1 JP 2021045714 W JP2021045714 W JP 2021045714W WO 2022138254 A1 WO2022138254 A1 WO 2022138254A1
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Prior art keywords
slide glass
slide
housing
storage
stage
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PCT/JP2021/045714
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English (en)
Japanese (ja)
Inventor
謙太 向井
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ソニーグループ株式会社
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Publication of WO2022138254A1 publication Critical patent/WO2022138254A1/fr

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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides

Definitions

  • This disclosure relates to a slide glass storage rack, a slide storage, and a slide glass photographing system.
  • a microscope system that can automatically perform digital imaging on a slide glass carrying a biological specimen (for example, cell tissue, etc.) has been developed.
  • a large number of slide glasses are prepared in advance in the slide storage, and the slide handler (slide glass transport device) takes out the slide glasses one by one from the slide storage and transports them to the stage for photographing. Repeat that. By doing so, it is possible to automatically perform microscopic photography of a large amount of slide glass without bothering human hands.
  • the slide storage can accommodate a plurality of cassettes (slide glass storage racks) for storing a plurality of slide glasses.
  • cassettes semiconductor glass storage racks
  • this disclosure proposes a slide glass storage rack, a slide storage, and a slide glass photographing system that can improve the efficiency of the slide glass replacement work.
  • a slide glass storage rack for storing a slide glass on which a biological specimen is mounted, a housing having a storage space for storing a plurality of the slide glasses and having an opening on the front surface, and a housing.
  • a pair of pairs of storage spaces partitioned in a vertical direction into a plurality of stages and separated from each other by a predetermined distance along a second direction from one side surface of the housing to the other side surface.
  • Each of the slide glasses is provided with an arm member, and each of the slide glasses is housed in the storage space by being bridged between the pair of arm members, and each of the arm members protrudes from the opening of the housing.
  • a slide glass storage rack is provided in which the length of the arm member in the first direction from the back surface of the housing to the front surface is different in each stage.
  • the slide storage is for detachably accommodating a plurality of slide glass storage racks
  • the slide glass storage rack has a storage space for accommodating a plurality of slide glasses on which a biological specimen is mounted.
  • the housing having an opening on the front surface and the storage space are divided into a plurality of stages along the vertical direction, and along a second direction from one side surface of the housing to the other side surface. It has a plurality of pairs of arm members separated from each other by a predetermined distance, and each of the slide glasses is housed in the storage space by being bridged between the pair of arm members.
  • Each of the members projects from the opening of the housing, and the length of the arm member in the first direction from the back surface of the housing to the front surface is different in each stage to provide slide storage.
  • a slide glass photographing system including a slide transport unit for transporting a slide glass, wherein the slide storage detachably accommodates a plurality of slide glass storage racks, and the slide glass storage rack includes a plurality of the slides.
  • a housing having a storage space for storing glass and having an opening on the front surface, and the storage space being divided into a plurality of stages along the vertical direction, and one side surface to the other side surface of the housing.
  • each of the slide glass slides is bridged between the pair of arm members.
  • Each of the arm members housed in the storage space protrudes from the opening of the housing, and the length of the arm member in the first direction from the back surface of the housing to the front surface is set in each of the stages.
  • a tissue section or cell that is a part of a tissue obtained from a living body (for example, a human body, an animal, a plant, etc.) is referred to as a biological sample (sample). Called.
  • the biological specimen described below may be stained in various ways as needed. In other words, in the embodiments of the present disclosure described below, the biological specimen may or may not be stained with various stains, unless otherwise specified.
  • the staining includes not only general staining represented by HE (hematoxylin / eosin) staining, gymza staining, papanicolou staining, etc., but also periodic acid shift (PAS) staining used when focusing on a specific tissue. It also includes fluorescent staining such as FISH (Fluorescence In-Situ Hybridization) and enzyme antibody method.
  • HE hematoxylin / eosin
  • PES periodic acid shift
  • FIG. 1 is an explanatory diagram for explaining the appearance of the slide glass 800 used in the photographing system 10 according to the embodiment of the present disclosure.
  • the slide glass 800 used in the imaging system 10 is a piece of glass cut into a rectangular shape, and a biological specimen to be observed is mounted on the central portion of the surface thereof. Will be done.
  • the slide glass 800 has a width along the X-axis direction (short direction) satisfying a predetermined dimensional standard, and has a width of, for example, 25 mm to 27 mm.
  • the slide glass 800 has a width along the Y-axis direction (longitudinal direction) satisfying a predetermined dimensional standard, and has a width of, for example, 75 mm to 77 mm.
  • the slide glass 800 has a film thickness satisfying a predetermined dimensional standard, for example, a film thickness of 0.90 mm to 1.20 mm. Further, as shown in FIG. 1, the slide glass 800 has end faces 804a and 804b extending along the X-axis direction and facing each other, and extending along the Y-axis direction of FIG. 1 and facing each other end faces 802a and 802b. Have.
  • a region called a frost portion 806 on which a biological specimen is not mounted for example, a region called a frost portion 806 on which a biological specimen is not mounted, and for example, information for identifying the biological specimen is described.
  • a possible area may be provided.
  • FIG. 2 is an explanatory diagram for explaining a configuration example of the photographing system 10 according to the embodiment of the present disclosure.
  • the imaging system 10 according to the present embodiment is a microscope system capable of digitally photographing a slide glass 800 on which a biological specimen (for example, a cell tissue or the like) is mounted.
  • the imaging system 10 includes a slide storage 100 for storing a plurality of slide glasses 800 on which a biological specimen is mounted. Further, the photographing system 10 according to the present embodiment supports each slide glass 800 between the photographing block (imaging unit) 200 for digitally photographing each slide glass 800 and the slide storage 100 and the photographing block 200. , Mainly includes a slide handler (slide transport unit) 300 for transport. In addition, the imaging system 10 further includes a control unit (not shown).
  • the slide handler 300 can automatically control and transport the slide glass 800. Therefore, the photographing system 10 according to the present embodiment can automatically perform digital imaging on several hundred slide glasses 800 stored in the slide storage 100 in a short time. For example, by using the imaging system 10 according to the present embodiment, it is possible to automatically capture a large amount of the slide glass 800 on which the biomedical specimen is mounted even at night when the inspection engineer is absent. It can shorten the diagnosis time and make up for the shortage of laboratory technicians.
  • the details of each device included in the photographing system 10 according to the present embodiment will be described.
  • FIG. 3 is an explanatory diagram for explaining the appearance of the slide storage 100 according to the present embodiment.
  • the slide storage 100 can accommodate a plurality of cassettes (slide glass storage racks) 400, each of which stores a plurality of slide glass 800.
  • the cassette 400 can be detachably attached to, for example, a space on the side surface of the storage portion 102 of the columnar slide storage 100.
  • the storage unit 102 can rotate around the central axis of the cylinder as a rotation axis, and the rotation is controlled by a control unit (not shown) described later.
  • the cassette 400 houses each slide glass 800 so as to be parallel to the horizontal plane, and in detail, any of the end faces 802a and 802b (see FIG.
  • the slide glass 800 in the Y-axis direction (longitudinal direction).
  • the slide glass 800 is stored so that it faces outward. More specifically, the slide glass 800 housed in the cassette 400 is taken out one by one by the slide handler 300 and transported to the photographing block 200.
  • the slide glass 800 is stored in such a cassette 400, the biological specimen is mounted on the slide glass 800, and the biological specimen is enclosed in a cover glass (not shown).
  • the adjustment work can be performed in a place different from the place where the photographing system 10 is installed (for example, a clean room, a decompression room, etc.). That is, according to the present embodiment, the adjusted slide glass 800 can be stored in the cassette 400 and carried to the place where the photographing system 10 is installed. The details of the cassette 400 according to this embodiment will be described later.
  • FIG. 4 is an explanatory diagram for explaining the appearance of the photographing block 200 according to the present embodiment.
  • the photographing block 200 has a stage 202 on which the slide glass 800 is placed.
  • the stage 202 can move in a direction parallel to the horizontal plane (X-axis direction or Y-axis direction) with the slide glass 800 placed therein, and is further vertical to the horizontal plane (Z-axis direction). ) Can be moved.
  • the stage 202 is moved by the stage drive mechanism 204 controlled by a control unit (not shown) described later.
  • a light source (not shown) that irradiates light from below the slide glass 800 placed on the stage 202 may be provided in the stage drive mechanism 204.
  • the light emitted from the light source is not particularly limited, and may be, for example, red, blue, or green visible light, white light, or infrared light. , Is not particularly limited.
  • the photographing block 200 has a microscope 206 for photographing a biological specimen mounted on the slide glass 800 mounted on the stage 202.
  • the microscope 206 mainly has an image sensor (not shown) and a lens system (not shown).
  • the lens system comprises a plurality of lenses including an objective lens and an imaging lens, and a biological specimen on a slide glass 800 illuminated by a light source (not shown) of the stage 202 can be magnified to a predetermined magnification.
  • An image of a biological specimen can be imaged on an image sensor.
  • the image sensor can be realized by, for example, an image pickup device such as a CCD (Charge Coupled Device) or a CMOS (Complementary Metal Oxide Semiconductor).
  • the image sensor is a line sensor that captures an imaging range consisting of a predetermined horizontal width and vertical width by repeating imaging of a thin rectangular area together with its own movement or movement of the stage 202. May be.
  • the image sensor captures a shooting range (for example, a shooting area of several mm ⁇ several mm) having a predetermined width and height without repeating the movement of itself or the movement of the stage 202. It may be an area sensor.
  • the image data acquired by the image sensor is transmitted to, for example, a control unit (not shown) described later, processed, or output to another device (for example, a display device, etc.). can do.
  • the slide glass 800 conveyed from the slide storage 100 by the slide handler 300 is placed on the stage 202, so that the biological specimen mounted on the slide glass 800 is photographed.
  • FIG. 5 is an explanatory diagram for explaining the appearance of the slide handler 300 according to the present embodiment.
  • the slide handler 300 As shown in FIG. 5, the slide handler 300 according to the present embodiment is provided so as to be movable along a predetermined direction on a plane, and a contact member 318 that abuts on the end surface 802a of the slide glass 800 is provided at the tip end portion. It has a catcher arm 316 to have. Further, the slide handler 300 has two contact members 322 of the slide glass 800 that come into contact with the other end surface 802b facing the end surface 802a in the predetermined direction, and has a handler 320 that supports the slide glass 800 from below. Have. Further, in the present embodiment, the handler 320 is provided so as to be movable along the predetermined direction by a drive unit (not shown).
  • the slide glass 800 can be gripped by moving the catcher arm 316 to a position where the contact member 318 and the contact member 322 abut at the same time as the slide glass 800. Further, in the present embodiment, since the slide glass 800 is supported from below by the handler 320, it is difficult to drop the slide glass 800 during transportation. In addition, in the present embodiment, since the slide glass 800 is supported from below by the handler 320, even if the thickness of the slide glass 800 varies widely, each slide glass 800 can be used. Can be properly gripped.
  • the slide handler 300 has a guide mechanism 350 that guides the slide glass 800 so as to be located at the center of the handler 320. According to the present embodiment, by providing such a guide mechanism, it is possible to correct the posture deviation due to the transportation of the slide glass 800, so that the accuracy of delivery of the slide glass 800 to the photographing block 200 and the slide storage 100 can be improved. Can be improved.
  • the photographing system 10 includes a control unit (not shown) as described above.
  • the control unit controls each device of the photographing system 10, and performs predetermined image processing or the like on the image data obtained by the photographing, and the image data subjected to the image processing is the other device (for example, a display device). ), Or can be stored in a predetermined storage unit (not shown).
  • control unit includes, for example, a CPU (Central Processing Unit) that performs various arithmetic processes, a ROM (Read Only Memory) in which data is stored in advance, and a RAM (Random Access Memory) in which data is temporarily stored. It is mainly composed of a control unit that has. Further, the control unit has an interface capable of exchanging control signals and various data with the slide storage 100, the shooting block 200, and the slide handler 300.
  • CPU Central Processing Unit
  • ROM Read Only Memory
  • RAM Random Access Memory
  • the photographing system 10 and the devices included in the photographing system 10 according to the present embodiment are not limited to those shown in FIGS. 2 to 5, and for example, further devices. Alternatively, it may contain other elements.
  • a surgeon collects a piece of tissue from the patient's body, a pathologist observes the piece of tissue collected under a microscope, and the tissue with a suspected cancer lesion is malignant. May be diagnosed as benign or benign.
  • the number of cancer patients has increased, and the number of cases of making a diagnosis by a pathologist as described above is increasing.
  • pathologists and laboratory technicians are limited, and the work of pathological diagnosis is always required to be performed accurately and smoothly, pathologists and laboratory technicians are always under pressure. It can be said that it is in a state.
  • the cassette 400 used in the above-mentioned imaging system 10 has the following problems. I understood.
  • the conventional cassette 400 has a storage space for storing a plurality of slide glasses 800, and has a housing having an opening on the front surface, and a plurality of storage spaces in the storage space are provided along the vertical direction. It has multiple shelves that partition into the steps of.
  • the shelf board has a slit in the center thereof, and the slide glass 800 can be stored in the storage space by being placed so as to be bridged over the slit.
  • the biological specimen mounted on the slide glass 800 is enclosed by being covered with the cover glass, and an encapsulant (adhesive) may be used to fix the cover glass. Therefore, the slit provided in the central portion of the shelf board is provided to prevent the encapsulant in a dry state from adhering to the shelf board. Further, by providing the slit, it is possible to visually confirm the state of the biological specimen on the slide glass 800 even when it is stored in the cassette 400.
  • the present inventor has created the embodiment of the present disclosure described below in order to improve the efficiency of the replacement work of the slide glass 800.
  • the cassette 400 according to the embodiment of the present disclosure described below mainly has four elements in order to improve the efficiency of the replacement work of the slide glass 800.
  • the first element makes it easier for pathologists, laboratory technicians, etc. to visually recognize each stage in the storage space of the cassette 400, and the adjusted slide glass 800 is inserted diagonally across the stages. Can be prevented.
  • the second element makes it easier to see each stage.
  • the third element can prevent the slide glass 800 from falling from the cassette 400.
  • the fourth element makes it possible to more efficiently insert the slide glass 800 into the cassette 400.
  • such four elements will be sequentially described as the first to fourth embodiments of the present disclosure.
  • FIG. 6 is a front view of the cassette 400 according to the present embodiment
  • FIG. 7 is a side view of the cassette 400 according to the present embodiment
  • FIG. 8 is a perspective view of the cassette 400 according to the present embodiment, and more specifically, is a perspective view of the cassette 400 in a state where the slide glass 800 is housed.
  • FIG. 9 is a partially enlarged view of the cassette 400 according to the present embodiment, and more specifically, is a partially enlarged view of FIG. 7.
  • the cassette 400 has a housing having a storage space for accommodating a plurality of slide glasses 800.
  • the housing has a top plate 406, side plates 402, 404, a bottom plate 408, and a back plate 410, and the space surrounded by these parts is a storage space for accommodating a plurality of slide glasses 800. Further, the entire surface of the housing facing the back plate 410 is open, and the slide glass 800 can be inserted into the storage space through the opening.
  • the width of the housing (width along the Y-axis direction in the figure) is about 81 mm, and the height (height along the Z-axis direction (vertical direction) in the figure) is about 150 mm. Is preferable. Since the cassette 400 is so small, it can be carried with one hand. Further, since the cassette 400 can be placed on the desk of the pathological diagnosis room for adjusting the slide glass 800, it is easy to secure the installation space. In this embodiment, a handle (not shown) may be provided on the upper surface plate of the housing, and by doing so, it is possible to make it easier to carry.
  • the cassette 400 has a plurality of shelf board members that partition the storage space into a plurality of stages along the Z-axis direction in the drawing.
  • a slide glass 800 is placed on each of the shelf board members. That is, the shelf board member functions as a shelf that supports the slide glass 800.
  • the height of the space existing on each step is preferably about 6 mm, and by doing so, the finger Even a thick person can significantly insert the slide glass 800 without the finger colliding with the shelf board member.
  • each of the above-mentioned shelf board members is in the Y-axis direction (second) in the drawing from one side surface of the housing to the other side surface. It is composed of a pair of arm members 420 separated from each other by a predetermined distance along the direction).
  • Each slide glass 800 is housed in the storage space by being bridged between each pair of arm members 420.
  • by providing a space in the center of each stage sandwiched between the pair of arm members 420 in this way it is possible to prevent the encapsulant in a dry state from adhering to the arm member 420. can. Further, in the present embodiment, by providing such a space, it is possible to visually confirm the state of the biological specimen on the slide glass 800 even when it is stored in the cassette 400.
  • the arm member 420 protrudes from the opening of the housing.
  • the lengths of the side plates 402 and 404 in the X-axis direction (first direction) in the drawing from the back surface to the front surface of the housing are the lengths of the slide glass 800 in the X-axis direction (short side). Direction) (see Fig. 1) is shorter than the length.
  • the slide glass 800 is placed on the pair of arm members 420. At least a part of the end faces 804a and 804b (see FIG. 1) of the slide glass 800 is exposed from the housing. In the present embodiment, by doing so, in the work of sandwiching the end faces 804a and 804b of the slide glass 800 with fingers and inserting the slide glass 800 into the cassette 400, the fingers collide with the housing and the cassette. It is possible to avoid the occurrence of an accident such as the 400 falling down. As a result, according to the present embodiment, the efficiency of the replacement work of the slide glass 800 is improved.
  • each arm member 420 in the X-axis direction in the figure is different in each stage. Specifically, in the present embodiment, as shown in FIG. 9, an odd-numbered arm member 420b (arm member 420a in FIGS. 6 and 8) and an even-numbered arm member 420d (FIGS. 6 and 8) are counted from the top. Its length is different from that of the arm member 420c) in FIG. More specifically, in the example shown in FIG. 9, the odd-numbered arm member 420b (arm member 420a in FIGS. 6 and 8) is replaced with the even-numbered arm member 420d (arm member 420c in FIGS. 6 and 8). Compared to short.
  • the present embodiment it becomes easy to visually distinguish each adjacent stage due to the difference in length. Therefore, a pathologist, an examination engineer, or the like can use each slide glass 800. It will be easy to insert and place it properly on the stage. Therefore, according to the present embodiment, it is possible to avoid inserting the adjusted slide glass 800 diagonally across the steps and damaging the biological specimen on the slide glass 800. As a result, according to the present embodiment, the efficiency of the replacement work of the slide glass 800 can be improved.
  • the odd-numbered arm member 420b (arm member 420a in FIGS. 6 and 8) has an even-numbered arm member 420d (arm member 420c in FIGS. 6 and 8).
  • the odd-numbered arm member 420b (arm member 420a in FIGS. 6 and 8) is the even-numbered arm member 420d (arm member 420c in FIGS. 6 and 8). It may be longer than.
  • each arm member 420 is provided so that the length of each arm member 420 in the X-axis direction in the figure gradually increases from the upper stage to the lower stage along the Z-axis direction in the figure. May be.
  • each arm member 420 is provided so that the length of each arm member 420 in the X-axis direction in the figure gradually shortens from the upper stage to the lower stage along the Z-axis direction in the figure. May be.
  • the lengths of the arm members 420 in the X-axis direction in the drawing may be different between adjacent stages. By doing so, in the present embodiment, it becomes easy to visually distinguish the adjacent steps due to the difference in length.
  • the guide line 430 shown in FIG. 6, the identifier 432, the protrusions 412, 414, 416 shown in FIGS. 7 and 8, and the locking portion 422 shown in FIG. 9 are described later in the present disclosure. This will be described in the embodiment.
  • FIG. 10 is a partially enlarged view of the cassette 400 according to the present embodiment, and is a partially enlarged view of FIG. 6 in detail.
  • the Y-axis direction (second direction) (second direction) ( A guide line 430 extending along (see FIG. 6) is provided.
  • the guide line 430 can be formed by unevenness extending along the Y-axis direction on the inner surface of the back plate 410.
  • the guide line 430 makes it easy to visually identify the adjacent steps. Therefore, a pathologist, an examination engineer, or the like can use the slide glass 800. It becomes easy to appropriately insert and place it in each stage along the guide line 430. Therefore, according to the present embodiment, it is possible to avoid inserting the adjusted slide glass 800 diagonally across the steps and damaging the biological specimen on the slide glass 800. As a result, according to the present embodiment, the efficiency of the replacement work of the slide glass 800 can be improved. In this embodiment, by forming the guide wire 430 with unevenness, the guide wire 430 is not lost even if the cassette 400 is washed a plurality of times with a liquid or the like.
  • the guide line 430 is not limited to being formed by unevenness, and the guide line 430 may be formed by printing on the inner surface of the back plate 410.
  • the guide line 430 can be formed by using highly visible colors, so that the visibility of the guide line 430 can be further improved. Further, in such a case, by constructing the guide line 430 using different colors in the adjacent stages, it is possible to make it easier to visually distinguish the adjacent stages.
  • the identifier 432 for identifying each stage is provided in the area sandwiched between the pair of arm members 520 inside the back plate 410 of the housing of the cassette 400.
  • the identifier 432 is a number sequentially attached to each stage from the upper stage to the lower stage in the Z-axis direction (vertical direction) (see FIG. 6), and is formed by the unevenness on the inner surface of the back plate 410. can do.
  • the identifier 432 is provided on the guide line 430 described above.
  • the stage in which the slide glass 800 is housed can be easily visually recognized by the identifier 432, so that the target slide glass 800 can be instantly used when an error occurs or the like. Can be recognized and dealt with. As a result, according to the present embodiment, the efficiency of the replacement work of the slide glass 800 can be improved.
  • the numbers sequentially attached to each stage are not limited to being provided only on the inside of the back plate 410, and at the same time, each stage on the outer surface of the side plates 402 and 404. It may be provided at the position corresponding to.
  • the identifier 432 is not limited to being a number, and may be a predetermined mark or the like, and is not particularly limited.
  • the identifier 432 by forming the identifier 432 by the unevenness, the identifier 432 is not lost even if the cassette 400 is washed a plurality of times with a liquid or the like.
  • the identifier 432 is not limited to being formed by unevenness, and the identifier 432 may be formed by printing on the inner surface of the back plate 410. By forming the identifier 432 by such printing, the identifier 432 can be configured by using highly visible colors, so that the visibility of the identifier 432 can be further improved.
  • FIG. 11 is a partially enlarged view of the cassette 400 according to the present embodiment, and in detail, is a partially enlarged view of FIG.
  • each arm member 420 has a locking portion 422 that is located at the tip of each arm member 420 and projects upward.
  • the side surfaces of the locking portion 422 come into contact with the end faces 802a and 802b (see FIG. 1) of the slide glass 800 mounted on the pair of arm members 420, so that the slide glass 800 is paired. It has a function of preventing the arm member 420 from falling from the arm member 420.
  • by providing such a locking portion 422 it is possible to prevent the slide glass 800 from falling, so that the efficiency of the replacement work of the slide glass 800 can be improved.
  • the height of the locking portion 422 from the upper surface of the arm member 420 is 0.85 mm or more and 1.25 mm or less in consideration of the dimensional variation in the film thickness of the slide glass 800. preferable.
  • FIG. 12 is a top view of the cassette 400 according to the present embodiment
  • FIG. 13 is a perspective view of the cassette 400 according to the present embodiment
  • FIG. 14 is an explanatory diagram for explaining an embodiment.
  • the cassette 400 may be placed horizontally (the cassette 400 is placed on the desk so that the height direction of the cassette 400 is parallel to the horizontal plane and the opening faces upward). be.
  • the work efficiency may be improved.
  • the cassette 400 when the cassette 400 is placed horizontally, the cassette 400 is configured to be tilted so that the slide glass 800 is closer to one side plate 402 side. By doing so, it is possible to prevent the finger from colliding with the housing of the cassette 400 when the frosted portion 806 of the slide glass 800 is pinched by the pad of the finger and inserted into the cassette 400. Therefore, according to the present embodiment, it is possible to avoid an accident such as a finger colliding with the housing and the cassette 400 falling from the desk. As a result, according to the present embodiment, the efficiency of the replacement work of the slide glass 800 is improved.
  • the cassette 400 according to the present embodiment has two different lengths on the back end faces of the upper surface plate 406 and the lower surface plate 408 of the housing of the cassette 400.
  • Protrusions 412, 414, 416, 418 are provided.
  • the protrusion 412 of the upper surface plate 406 is longer than the protrusion 414 of the upper surface plate 406, and the protrusion 416 of the lower surface plate 408 is a protrusion of the lower surface plate 408. Longer than part 418.
  • the end faces 802a and 802b (see FIG. 1) of the slide glass 800 in the longitudinal direction (Y-axis direction) are 5 degrees or more and 45 degrees with respect to the upper surface of the desk 700. It is preferable to have the following angles (predetermined angles), and it is more preferable to have an angle of about 15 degrees.
  • the protrusion 412 of the upper surface plate 406 is longer than the protrusion 414 of the upper surface plate 406, and the protrusion 416 of the lower surface plate 408 is the lower surface plate.
  • the configuration is not limited to a longer structure than the protrusion 418 of the 408.
  • the protrusion 412 of the upper surface plate 406 may be shorter than the protrusion 414 of the upper surface plate 406, and the protrusion 416 of the lower surface plate 408 may be shorter than the protrusion 418 of the lower surface plate 408. That is, in the present embodiment, the protrusions of the upper surface plate 406 and the lower surface plate 408 located on one side plate side may be longer than the protrusions located on the other side plate side.
  • the end faces on the back surface side of the upper surface plate 406 and the lower surface plate 408 of the housing of the cassette 400 may be configured to have a predetermined angle with respect to the end faces on the front surface side.
  • the predetermined angle is preferably 5 degrees or more and 45 degrees or less, and more preferably about 15 degrees, in view of work efficiency and the like. preferable.
  • the cassette 400 when the cassette 400 is placed horizontally, the cassette 400 can be tilted so that the slide glass 800 is closer to one side plate 402 side.
  • the frosted portion 806 of the slide glass 800 when the frosted portion 806 of the slide glass 800 is pinched by the pad of the finger and inserted into the cassette 400, the finger collides with the housing of the cassette 400. Can be avoided. Therefore, it is possible to avoid an accident such as the cassette 400 falling off the desk due to a finger colliding with the housing. As a result, according to this modification, the efficiency of the replacement work of the slide glass 800 is improved.
  • the efficiency of the replacement work of the slide glass 800 can be improved.
  • the pathologist, the laboratory engineer, or the like can easily proceed with the work accurately and smoothly, so that the tight state of the pathologist or the like can be alleviated. That is, according to the present embodiment, it is possible to shorten the examination and diagnosis time, reduce the labor burden of the pathologist, the examination engineer, etc., and compensate for the shortage of the pathologist, the examination engineer, etc.
  • the object to be imaged is not limited to the biological specimen mounted on the slide glass 800, but is an observed object having the same shape as the slide glass 800, or a slide glass. Any object to be observed may be mounted on a substrate (not shown) having the same shape as the 800, and is not particularly limited. Further, the above-described embodiment of the present disclosure is not limited to application to applications such as medical treatment or research, and is particularly limited to applications that require high-precision analysis or the like using images. Not limited.
  • the technique according to the present disclosure can be applied to various products. For example, even if the technique according to the present disclosure is applied to a pathological diagnosis system or a support system thereof (hereinafter referred to as a diagnosis support system) in which a doctor or the like observes cells or tissues collected from a patient to diagnose a lesion. good.
  • This diagnostic support system may be a WSI (Whole Slide Imaging) system that diagnoses or supports a lesion based on an image acquired by using a digital pathology technique.
  • FIG. 15 is a diagram showing an example of a schematic configuration of a diagnostic support system 5500 to which the technique according to the present disclosure is applied.
  • the diagnostic support system 5500 includes one or more pathological systems 5510. Further, the diagnostic support system 5500 may include a medical information system 5530 and a derivation device 5540.
  • Each of the one or more pathological systems 5510 is a system mainly used by pathologists, and is introduced into, for example, a laboratory or a hospital.
  • Each pathological system 5510 may be introduced in different hospitals, and may be installed in various networks such as WAN (Wide Area Network) (including the Internet), LAN (Local Area Network), public line network, and mobile communication network, respectively. It is connected to the medical information system 5530 and the out-licensing device 5540 via the system.
  • WAN Wide Area Network
  • LAN Local Area Network
  • public line network public line network
  • mobile communication network mobile communication network
  • Each pathological system 5510 includes a microscope (specifically, a microscope used in combination with digital imaging technology) 5511, a server 5512, a display control device 5513, and a display device 5514.
  • a microscope specifically, a microscope used in combination with digital imaging technology
  • server 5512 a server 5512
  • display control device 5513 a display device 5514.
  • the microscope 5511 has the function of an optical microscope, photographs an observation object housed in a glass slide, and acquires a pathological image which is a digital image.
  • the observation object is, for example, a tissue or cell collected from a patient, and may be a piece of meat, saliva, blood, or the like of an organ.
  • the microscope 5511 functions as the imaging system 10 shown in FIG.
  • the server 5512 stores and stores the pathological image acquired by the microscope 5511 in a storage unit (not shown). Further, when the server 5512 receives a viewing request from the display control device 5513, the server 5512 searches for a pathological image from a storage unit (not shown) and sends the searched pathological image to the display control device 5513.
  • the server 5512 may function as the control unit according to the embodiment of the present disclosure.
  • the display control device 5513 sends a viewing request for the pathological image received from the user to the server 5512. Then, the display control device 5513 displays the pathological image received from the server 5512 on the display device 5514 using a liquid crystal display, EL (Electro-Luminence), CRT (Cathode Ray Tube), or the like.
  • the display device 5514 may be compatible with 4K or 8K, and is not limited to one, and may be a plurality of display devices.
  • the object to be observed when the object to be observed is a solid substance such as a piece of meat of an organ, the object to be observed may be, for example, a stained thin section.
  • the thin section may be prepared, for example, by slicing a block piece cut out from a sample such as an organ. Further, when slicing, the block pieces may be fixed with paraffin or the like.
  • Staining of thin sections includes general staining showing the morphology of the tissue such as HE (Hematoxylin-Eosin) staining, immunostaining showing the immune status of the tissue such as special staining, IHC (Immunohistochemistry) staining, and fluorescent immunostaining. Staining may be applied. At that time, one thin section may be stained with a plurality of different reagents, or two or more thin sections (also referred to as adjacent thin sections) continuously cut out from the same block piece may be different reagents from each other. It may be stained using.
  • the microscope 5511 may include a low-resolution photographing unit for photographing at a low resolution and a high-resolution photographing unit for photographing at a high resolution.
  • the low-resolution photographing unit and the high-resolution photographing unit may have different optical systems or may be the same optical system. When the optical system is the same, the resolution of the microscope 5511 may be changed according to the object to be photographed.
  • the slide glass 800 containing the observation object is placed on a stage located within the angle of view of the microscope 5511.
  • the microscope 5511 acquires an entire image within the angle of view using a low-resolution photographing unit, and identifies an area of an observation object from the acquired overall image.
  • the microscope 5511 divides the area where the observation object exists into a plurality of divided areas of a predetermined size, and sequentially photographs each divided area by the high-resolution photographing unit to acquire a high-resolution image of each divided area. do.
  • the stage may be moved, the photographing optical system may be moved, or both of them may be moved.
  • each divided region may overlap with the adjacent divided region in order to prevent the occurrence of a shooting omission region due to an unintended slip of the glass slide.
  • the whole image may include identification information for associating the whole image with the patient. This identification information may be, for example, a character string, a QR code (registered trademark), or the like.
  • the high resolution image acquired by the microscope 5511 is input to the server 5512.
  • the server 5512 divides each high-resolution image into smaller-sized partial images (hereinafter referred to as tile images). For example, the server 5512 divides one high-resolution image into a total of 100 tile images of 10 ⁇ 10 vertically and horizontally. At that time, if the adjacent divided regions overlap, the server 5512 may perform stitching processing on the high-resolution images adjacent to each other by using a technique such as template matching. In that case, the server 5512 may generate a tile image by dividing the entire high-resolution image bonded by the stitching process. However, the tile image may be generated from the high resolution image before the stitching process.
  • the server 5512 can generate a tile image of a smaller size by further dividing the tile image. The generation of such a tile image may be repeated until a tile image having a size set as a minimum unit is generated.
  • the server 5512 executes the tile composition process of generating one tile image by synthesizing a predetermined number of adjacent tile images for all the tile images. This tile composition process can be repeated until one tile image is finally generated.
  • a tile image group having a pyramid structure in which each layer is composed of one or more tile images is generated.
  • the tile image of one layer and the tile image of a different layer have the same number of pixels, but their resolutions are different. For example, when a total of four tile images of 2 ⁇ 2 are combined to generate one tile image in the upper layer, the resolution of the tile image in the upper layer is 1/2 times the resolution of the tile image in the lower layer used for composition. It has become.
  • a tile image group having such a pyramid structure By constructing a tile image group having such a pyramid structure, it is possible to switch the degree of detail of the observation object displayed on the display device depending on the hierarchy to which the tile image to be displayed belongs. For example, when the tile image of the lowest layer is used, the narrow area of the observation object may be displayed in detail, and the wider area of the observation object may be displayed coarser as the tile image of the upper layer is used. can.
  • the generated tile image group of the pyramid structure is stored in a storage unit (not shown) together with identification information (referred to as tile identification information) that can uniquely identify each tile image, for example.
  • the server 5512 receives a request for acquiring a tile image including tile identification information from another device (for example, a display control device 5513 or a derivation device 5540), the server 5512 transmits the tile image corresponding to the tile identification information to the other device. do.
  • the tile image which is a pathological image
  • a specific pathological image and another pathological image corresponding to an imaging condition different from the specific imaging condition, which is another pathological image in the same region as the specific pathological image are displayed. It may be displayed side by side.
  • Specific shooting conditions may be specified by the viewer. Further, when a plurality of imaging conditions are specified for the viewer, pathological images of the same region corresponding to each imaging condition may be displayed side by side.
  • the server 5512 may store the tile image group having a pyramid structure in a storage device other than the server 5512, for example, a cloud server. Further, a part or all of the tile image generation process as described above may be executed by a cloud server or the like.
  • the display control device 5513 extracts a desired tile image from the tile image group having a pyramid structure in response to an input operation from the user, and outputs this to the display device 5514.
  • the user can obtain the feeling of observing the observation object while changing the observation magnification. That is, the display control device 5513 functions as a virtual microscope.
  • the virtual observation magnification here actually corresponds to the resolution.
  • any method may be used for shooting a high-resolution image.
  • the divided area may be photographed while repeatedly stopping and moving the stage to acquire a high-resolution image, or the divided area may be photographed while moving the stage at a predetermined speed to acquire a high-resolution image on the strip. May be good.
  • the process of generating a tile image from a high-resolution image is not an indispensable configuration, and by gradually changing the resolution of the entire high-resolution image bonded by the stitching process, an image whose resolution changes stepwise can be created. It may be generated. Even in this case, it is possible to gradually present the user from a low-resolution image in a wide area to a high-resolution image in a narrow area.
  • the medical information system 5530 is a so-called electronic medical record system, and stores information related to diagnosis such as patient identification information, patient disease information, test information and image information used for diagnosis, diagnosis results, and prescription drugs.
  • a pathological image obtained by photographing an observation object of a patient can be once stored via the server 5512 and then displayed on the display device 5514 by the display control device 5513.
  • the pathologist using the pathological system 5510 makes a pathological diagnosis based on the pathological image displayed on the display device 5514.
  • the results of the pathological diagnosis made by the pathologist are stored in the medical information system 5530.
  • the derivation device 5540 can perform analysis on the pathological image. A learning model created by machine learning can be used for this analysis.
  • the derivation device 5540 may derive a classification result of a specific area, an organization identification result, or the like as the analysis result. Further, the derivation device 5540 may derive identification results such as cell information, number, position, and luminance information, and scoring information for them. These information derived by the derivation device 5540 may be displayed on the display device 5514 of the pathological system 5510 as diagnostic support information.
  • the out-licensing device 5540 may be a server system composed of one or more servers (including a cloud server) and the like. Further, the derivation device 5540 may be configured to be incorporated in, for example, a display control device 5513 or a server 5512 in the pathology system 5510. That is, various analyzes on the pathological image may be performed within the pathological system 5510.
  • the configuration described above can be applied not only to the medical diagnosis support system but also to all biological microscopes such as a confocal microscope, a fluorescence microscope, and a video microscope using digital imaging technology. That is, the configuration described above can be applied to various studies, analyzes, surveys, and the like.
  • the observation target may be a biological sample such as cultured cells, a fertilized egg, or a sperm, a biological material such as a cell sheet or a three-dimensional cell tissue, or a biological material such as a zebrafish or a mouse.
  • a moving image may be generated from a still image of an observation object acquired by using a microscope using a digital photographing technique.
  • a moving image may be generated from still images taken continuously for a predetermined period, or an image sequence may be generated from still images taken at predetermined intervals.
  • the observation target such as the beat and elongation of cancer cells, nerve cells, myocardial tissue, sperm, movement such as migration, and the division process of cultured cells and fertilized eggs. It is possible to analyze the dynamic characteristics of objects using machine learning.
  • a slide glass storage rack that stores slide glass on which biological specimens are mounted.
  • a housing having a storage space for storing the plurality of the slide glasses and having an opening on the front surface, A pair of pairs of storage spaces partitioned in a vertical direction into a plurality of stages and separated from each other by a predetermined distance along a second direction from one side surface of the housing to the other side surface.
  • the arm member Equipped with Each of the slide glasses is housed in the storage space by being bridged between the pair of arm members.
  • Each of the arm members protrudes from the opening of the housing and The length of the arm member in the first direction from the back surface of the housing to the front surface is different in each stage.
  • the length of the arm member in the first direction differs between the even-numbered stage and the odd-numbered stage.
  • (3) The length of the arm member in the first direction gradually increases from the upper stage to the lower stage in the vertical direction.
  • (4) The length of the arm member in the first direction gradually shortens from the upper stage to the lower stage in the vertical direction.
  • a guide wire extending along the second direction is provided, according to the above (1) to (6).
  • (8) The slide glass storage rack according to (7) above, wherein the color of the guide line is different in each stage.
  • Any of the above (1) to (8), wherein an identifier for identifying each stage is provided in a region of the inside of the back plate of the housing, which is sandwiched between the pair of arm members.
  • a slide storage that detachably accommodates multiple slide glass storage racks.
  • the slide glass storage rack is A housing that has a storage space for storing multiple slide glasses on which biological specimens are mounted and has an opening on the front surface.
  • a pair of pairs of storage spaces partitioned in a vertical direction into a plurality of stages and separated from each other by a predetermined distance along a second direction from one side surface of the housing to the other side surface.
  • the arm member, Have Each of the slide glasses is housed in the storage space by being bridged between the pair of arm members.
  • Each of the arm members protrudes from the opening of the housing and The length of the arm member in the first direction from the back surface of the housing to the front surface is different in each stage.
  • Slide storage for storing multiple slide glasses with biological specimens, An imaging unit that photographs each of the slide glasses, and A slide transport unit that transports each slide glass between the slide storage and the photographing unit, Is a slide glass photography system, including
  • the slide storage accommodates a plurality of slide glass storage racks in a detachable manner.
  • the slide glass storage rack is A housing having a storage space for storing the plurality of the slide glasses and having an opening on the front surface, A pair of pairs of storage spaces partitioned in a vertical direction into a plurality of stages and separated from each other by a predetermined distance along a second direction from one side surface of the housing to the other side surface.
  • Each of the slide glasses is housed in the storage space by being bridged between the pair of arm members.
  • Each of the arm members protrudes from the opening of the housing and The length of the arm member in the first direction from the back surface of the housing to the front surface is different in each stage. Slide glass photography system.

Abstract

La présente invention concerne un casier de stockage de lames de verre (400) qui stocke une lame de verre (800) sur laquelle un échantillon biologique a été monté, le casier de stockage comprenant : un boîtier ayant un espace de stockage qui stocke une pluralité de lames de verre, et ayant une ouverture sur sa surface avant ; et une pluralité de paires d'éléments de bras (420) qui divisent l'intérieur de l'espace de stockage en une pluralité de niveaux le long de la direction verticale, et qui sont séparés l'un de l'autre d'une distance prescrite le long d'une seconde direction qui est orientée d'une surface latérale du boîtier vers une autre surface latérale de celui-ci, chaque lame de verre étant stockée dans l'espace de stockage en étant posée entre les paires d'éléments de bras, les éléments de bras faisant chacun saillie à partir de l'ouverture du boîtier, et les longueurs des éléments de bras dans une première direction qui est orientée à partir de la surface arrière du boîtier vers la surface avant de celui-ci différant dans chaque niveau.
PCT/JP2021/045714 2020-12-24 2021-12-10 Casier de stockage de lames de verre, dispositif de stockage de lames et système d'imagerie de lames en verre WO2022138254A1 (fr)

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US20040251796A1 (en) * 2003-06-12 2004-12-16 Wood Nathan P. Apparatus for removably retaining a slide within a cassette
JP2005506537A (ja) * 2001-10-19 2005-03-03 モノジェン インコーポレイテッド 汎用顕微鏡スライドカセット
WO2006098441A1 (fr) * 2005-03-17 2006-09-21 Hamamatsu Photonics K.K. Cassette a verres de preparation microscopique et etui a cassette
JP2007084111A (ja) * 2005-09-22 2007-04-05 Olympus Corp スライドガラスカセット
JP2009530667A (ja) * 2006-03-13 2009-08-27 アイコニシス インコーポレーテッド 顕微鏡スライド自動読み取りシステム
CN202522452U (zh) * 2012-03-20 2012-11-07 福州金域医学检验所有限公司 荧光玻片保存架
JP2014526712A (ja) * 2011-09-09 2014-10-06 ベンタナ メディカル システムズ， インコーポレイテッド イメージングシステム、カセッテ、およびこれを使用する方法
WO2019109020A1 (fr) * 2017-11-30 2019-06-06 Leica Biosystems Imaging, Inc. Rideau de lumière de sécurité pour désactiver une rotation de carrousel

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005506537A (ja) * 2001-10-19 2005-03-03 モノジェン インコーポレイテッド 汎用顕微鏡スライドカセット
US20040251796A1 (en) * 2003-06-12 2004-12-16 Wood Nathan P. Apparatus for removably retaining a slide within a cassette
WO2006098441A1 (fr) * 2005-03-17 2006-09-21 Hamamatsu Photonics K.K. Cassette a verres de preparation microscopique et etui a cassette
JP2007084111A (ja) * 2005-09-22 2007-04-05 Olympus Corp スライドガラスカセット
JP2009530667A (ja) * 2006-03-13 2009-08-27 アイコニシス インコーポレーテッド 顕微鏡スライド自動読み取りシステム
JP2014526712A (ja) * 2011-09-09 2014-10-06 ベンタナ メディカル システムズ， インコーポレイテッド イメージングシステム、カセッテ、およびこれを使用する方法
CN202522452U (zh) * 2012-03-20 2012-11-07 福州金域医学检验所有限公司 荧光玻片保存架
WO2019109020A1 (fr) * 2017-11-30 2019-06-06 Leica Biosystems Imaging, Inc. Rideau de lumière de sécurité pour désactiver une rotation de carrousel

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