WO2022124138A1 - Beautification method for promoting skin collagen generation - Google Patents

Abstract

The objective of the present invention is to provide a beautification method for promoting skin collagen generation.　Provided is a beautification method for promoting skin collagen generation, said method comprising applying a pulse current to the skin of a subject.

Description

A beauty method for promoting collagen production in the skin

The present invention relates to a cosmetological method for promoting collagen production in the skin.

In recent years, devices have been developed to achieve cosmetic effects such as collagen production using ultrasonic waves. Patent Document 1 is an ultrasonic beauty treatment apparatus having an electric function in the main body of the apparatus for generating an ultrasonic excitation mode that changes between the maximum output and the minimum output, and the DC output voltage from a DC power supply is variable. Disclose a device configured to allow it. Patent Document 2 is a treatment device including a high-frequency signal generation circuit that continuously and repeatedly changes the oscillation output of a high-frequency signal between a maximum value and a minimum value with the passage of time to give a high-frequency signal to an ultrasonic vibrator. To disclose. Patent Document 3 has a magnetic field generating portion that generates a magnetic field that irradiates the fibroblasts and collagen tissue inside the epidermis on the outside of the epidermis, and irradiates the fibroblasts and collagen tissue inside the epidermis with the magnetic field through the epidermis. By doing so, the collagen tissue inside the epidermis is contracted, and a beauty device having an anti-wrinkle effect is disclosed.

Japanese Patent No. 3312112 Japanese Patent No. 46333795 Japanese Patent No. 5605927

The present inventor has surprisingly found that application of a pulsed current to a skin cell promotes the collagen-producing ability of the cell. Therefore, the present invention provides a cosmetological method for promoting collagen production in the skin.

The present invention includes the following inventions.
(1) A cosmetic method for promoting collagen production in the skin, which comprises applying a pulse current to the target skin.
(2) The beauty method of (1), wherein the pulse current has a frequency in the range of 200 Hz to 5000 Hz.
(3) The beauty method of (2), wherein the current value of the pulse current is in the range of 100 to 1000 μA.
(4) The beauty method according to any one of (1) to (3), wherein a pulse current is applied to the target skin via a conductive material.
(5) The conductive material is between a flexible base layer, an adhesive layer attached to one side surface of the base layer and having adhesiveness to the user's skin, and the base layer and the adhesive layer. The beauty method according to (4), wherein the conductive layer is composed of a conductive layer arranged in the above, and the conductive layer is electrically coupled to an electrode to which a current is applied from a current generating portion.
(6) The beauty method according to (5), wherein the drug layer is further arranged between the conductive layer and the adhesive layer or on the surface of the adhesive layer.
(7) The cosmetological method of (5) or (6), wherein the adhesive layer contains a drug.
(8) The beauty method according to any one of (5) to (7), wherein microneedles are applied to the surface of the adhesive layer.
(9) The beauty method according to any one of (1) to (8) for promoting moisturization of the skin.
(10) The beauty method according to any one of (1) to (9) for improving wrinkles on the skin.
(11) The beauty method according to any one of (1) to (10), wherein the step of applying the pulse current to the target skin is performed for 1 to 120 minutes.
(12) The conductive material has first and second sheet-shaped electrodes that are independent of each other, and each of the first and second sheet-shaped electrodes is connected to a current generating portion through a conductive cable. The beauty method according to any one of (5) to (11).
(13) The conductive material includes one sheet-shaped electrode including a first electrode portion, a second electrode portion, and an insulating portion provided between the first and second electrode portions. The single sheet-shaped electrode has first and second terminals electrically connected to each of the first electrode portion and the second electrode portion, and the first and second terminals are provided. The beauty method according to any one of (5) to (11), wherein the terminal is connected to the current generating portion.
(14) The beauty method is carried out by using a beauty device, and the beauty device is
It is equipped with a device unit and a sheet-shaped skin electrode that contacts the user's skin and gives electrical stimulation.
The device unit is a current generation unit for generating a pulse current, a control unit for controlling the current generation unit, and a skin electrode connection electrode for electrically connecting the current generation unit and the skin electrode. And a ground electrode for electrically connecting between the current generating part and the skin,
The skin electrode is made of a conductive material that applies the current from the current generating part to the skin via the sheet connection electrode.
When the beauty device is attached to the user's skin, the ground electrode and skin electrode come into contact with the user's skin, and when a pulse current is generated from the power generation unit by operating the control unit, the ground electrode and skin electrode (1) to (11), which are electrically conducted through the user's skin and a pulse current is applied to the user's skin to promote collagen production in the skin of the wearing portion. Any beauty method.
(15) Further provided with a hollow housing for accommodating the current generation unit, the control unit, the current generation unit, and the power supply for supplying electric power to the control unit.
The hollow housing has a flat plate-shaped bottom wall, and the sheet connecting electrode and the ground electrode are arranged on the outer surface of the bottom wall.
The beauty method according to (14), wherein the sheet connection electrode and the ground electrode are in direct contact with the skin electrode and the user's skin when worn on the user.
(16) The cosmetic method of (15), wherein the hollow housing comprises a bottom wall and a closure coupled along an outer peripheral edge of the bottom wall.

By giving a pulse wave with a predetermined frequency, collagen production is promoted, and it becomes possible to moisturize and improve wrinkles.

Example 1 of an apparatus applied to the target skin via a conductive material. Example 2 of an apparatus applied to the target skin via a conductive material. It is a perspective view which shows the state at the time of wearing the beauty apparatus by a preferable embodiment of this invention. It is a side view of the device body of a beauty device. It is a bottom view of the mounting body of FIG. It is a schematic diagram of the inside of a mounting body which shows the closed part of a housing opened. It is sectional drawing of the mounting body along the arrow line-of-sight line VV of FIG. It is a schematic diagram which shows an example of the laminated structure of a sheet electrode. It is a schematic diagram which shows another example of the laminated structure of a sheet electrode. It is a schematic diagram which shows still another example of the laminated structure of a sheet electrode. Similar to FIG. 1, which shows a state in which the first electrode, which is the electrode for connecting the sheet, is brought into contact with the second portion 50b of the sheet electrode 50, and the second electrode, which is the electrode for skin, is brought into contact with the skin of the user. It is a perspective view. It is a chart which shows an example of the pulse wave generated by the pulse wave generation part. It is a perspective view which shows the state at the time of wearing the beauty apparatus by another preferable embodiment of this invention. It is a figure which shows the collagen amount (ng / cell) in Example 1. FIG. It is a figure which shows the change (au) of the water content of the skin in Example 2. FIG. It is a figure which shows the change of the wrinkle in Example 2. FIG. The figure below is an actual photograph of the skin taken with an ANTERA 3D camera, and the figure above is an image of the surface morphology of the skin portion corresponding to the figure below. It is a figure which shows the change of the depression index (Indentation Index) in Example 3. FIG. Shows the effect of reducing wrinkles on facial facial devices from other companies on the market. The comparison test result between the apparatus of this invention and the product of another company is shown.

The present invention relates to a method of applying a pulse wave having a predetermined frequency to the skin. By applying the cosmetological method of the present invention, it becomes possible to produce collagen in the skin, moisturize the skin, and improve wrinkles. When collagen production of the skin is promoted, moisturized, and wrinkles are improved, the skin is effective for moisturizing, maintaining elasticity and elasticity.

In one embodiment of the present invention, there is provided a cosmetic method comprising a step of applying an electrical stimulus to the skin by a pulse wave having a frequency in the range of 100 to 5000 Hz, more preferably 200 to 5000 Hz, still more preferably 500 to 2000 Hz. .. This cosmetological method may be performed by using a cosmetological device designed to apply a pulsed current to the skin.

The method of the present invention promotes the production of skin collagen. The measurement of the ability to produce skin collagen may be performed by a well-known method and is not particularly limited. For example, the amount of collagen in fibroblasts can be measured using, for example, a commercially available collagen measurement kit. For example, it can be measured by utilizing the fact that 3,4-DHPAA selectively binds to a peptide having glycine at the N-terminal and emits fluorescence, as in the collagen quantification kit of Cosmo Bio Co., Ltd. described in Examples. .. Collagen constituting the skin includes type I, type III, type IV, type V, type XII, type XIV and the like, and is not particularly limited in the present invention. good. The beauty method of the present invention is applied, for example, 1 to 480 minutes, 1 to 240 minutes, 1 to 180 minutes, 1 to 120 minutes, 20 to 180 minutes, 20 to 120 minutes, 60 to 120 minutes, and fibroblasts. The amount of collagen in skin cells such as cells is, for example, 1.1 times, 1.2 times, 1.3 times, 1.4 times, 1.5 times, 2.0 times, as compared with the case where nothing is applied. The effect of increasing by 3.0 times, 6.0 times, or more is achieved. Further, since the beauty method of the present invention can be applied for a long time, it is easy to apply a period longer than the above time. Further, such electrical stimulation may be performed a plurality of times.

The collagen production promoting effect of the present invention is achieved by applying a pulse wave to the skin at a constant frequency. The pulse wave is 100 Hz to 5000 Hz, preferably 200 Hz to 5000 Hz, and more preferably 500 Hz to 2000 Hz. The microcurrent generated by commercially available cosmetic devices and devices for wounds is about 0.1 Hz to 10 Hz, but the inventors of the present application apply skin collagen by applying a specific frequency higher than these. Was found to promote the production of.

In addition, commercially available facial equipment, massage equipment, and the like include those using electromagnetic waves having a frequency of about 0.3 to 300 MHz such as RF waves, and those using ultrasonic waves having a frequency of about 1 MHz to 7 MHz. Compared with these frequencies, the frequency adopted by the beauty method of the present invention is extremely low.

The current used to generate the pulse wave, for example, the alternating current can be arbitrarily selected from 100 μA to 1000 μA, 150 μA to 1000 μA, 200 μA to 500 μA, and the like. It is preferably 200 μA to 500 μA. Since the current value in such a range is equivalent to the current value in human skin, there is little adverse effect on the skin. The voltage can be arbitrarily selected from 1.0 V to 10.0 V, etc., 5.0 V to 10.0 V, etc., 1.0 V to 5.0 V, etc., within a range that does not adversely affect the skin. The pulse wave and voltage waveform can be arbitrarily set such as a pulse wave, a square wave, a sine wave, a triangular wave, and a sawtooth wave.

In the present invention, the pulse wave applied to the skin is 1 to 480 minutes, 1 to 240 minutes, 1 to 120 minutes, 5 to 120 minutes, 20 to 180 minutes, 20 to 120 minutes, 60 to 120 minutes, 20 to 60 minutes. It can be arbitrarily selected within a range that does not adversely affect the skin, such as minutes, 20 to 40 minutes. It is preferably 1 to 120 minutes. By applying the pulse wave of the present invention to the skin for the above time, collagen production in the skin is promoted, moisturization is promoted, and wrinkles are improved. In the beauty method of the present invention, the stimulus for the above time can be applied a plurality of times, such as twice, three times, four times, etc., or the stimulus for a time longer than the above time can be applied. A higher effect is expected by applying the stimulus for a longer time or multiple times. The frequency is daily, 2 days, 3 days, 4 days, 5 days, 6 days, 7 days, once every 1, 2, 3, 4 weeks, etc., continuously or intermittently, regularly or irregularly. It may be done in a targeted manner.

In one aspect, the cosmetological method of the present invention has the effect of promoting moisturizing of the skin. The moisturizing promoting effect can be measured, for example, the amount of water in the skin, the amount of transepidermal water loss (TEWL), or the like using an arbitrary skin water content checker such as a corneometer. For example, in the beauty method of the present invention, the pulse wave specified in the present invention is subjected to 1 to 480 minutes, 1 to 240 minutes, 1 to 120 minutes, 20 to 120 minutes, 60 to 120 minutes, 20 to 60 minutes, 20 to 40 minutes. When applied, for example, even if 15 minutes, 30 minutes, 45 minutes, 60 minutes, 90 minutes, 120 minutes, 480 minutes, 24 hours, 36 hours or more have passed after application, for example, before application of this device. The moisturizing promoting effect such that the water content of 35 au, 30 au, 25 au, 20 au, 15 au or more can be continuously maintained as compared with the value is exhibited. Further, since the beauty method of the present invention can be applied for a long time, further moisturizing promoting effect is expected.

In one aspect, the beauty method of the present invention has a wrinkle improving effect. The wrinkle improvement effect is obtained by visual inspection, judgment by a trained measurer, or image analysis, for example, image analysis of a photographic drawing taken by an Intera 3D camera (Gaderius) specifically shown in the embodiment of the present application. ) Can be measured. For example, when the beauty method of the present invention is applied for 1 to 480 minutes, 1 to 240 minutes, 1 to 120 minutes, 20 to 120 minutes, 60 to 120 minutes, 20 to 60 minutes, 20 to 40 minutes, 2 hours after application, Wrinkle improvement effect of 5 hours, 7 hours, 12 hours, 24 hours, 36 hours, 48 hours, 60 hours, 72 hours or more can be expected. For example, the beauty method of the present invention is applied for 20 minutes and repeated once, or twice, three times or more at specific time intervals such as 6 hours, 12 hours, 24 hours and 48 hours. Therefore, for example, according to the dent index by image analysis using an Antera 3D camera, a wrinkle improving effect such as a reduction of 5.0, 6.0, 7.0, 8.0, 9.0 or more can be achieved. Further, since the beauty method of the present invention can be applied for a long time, further wrinkle improving effect is expected.

The subject to which the method of the present invention is applied is a subject to which collagen deficiency, water content deficiency, and wrinkles are objectively or subjectively recognized, but it is desired to prevent collagen deficiency, water content deficiency, and wrinkles. It may be the desired object. For example, the subject may be a subject judged to be deficient in collagen or water content or deeply wrinkled, or may be a subject to be concerned about, for example, dry skin, wrinkles, swelling, or decreased elasticity. The amount of collagen, the amount of water, wrinkles, elasticity, elasticity and the like can be determined by using a known index such as visual judgment, subjective or objective evaluation, a cute meter value, or the above-mentioned method.

The collagen production promoting effect, moisturizing promoting effect, and wrinkle improving effect of the present invention can be measured not only by the above-mentioned methods but also by any method such as in vivo, in vitro, ex vivo, in situ.

The pulsed current can be applied to the skin of interest, for example via a conductive material.
In one embodiment, the conductive material has first and second sheet-like electrodes that are independent of each other, and each of the first and second sheet-like electrodes is connected to a current generating portion through a conductive cable. A pulsed current can be applied to the skin. An example thereof is shown in FIG.
In FIG. 1, the conductive material has first and second sheet-shaped electrodes 72a and 72b. The first and second sheet-shaped electrodes 72a and 72b are independent of each other and can be attached to a desired portion of the skin S by the user. The first and second sheet-shaped electrodes 72a and 72b are electrically connected to the current generating unit 70 by the cable 76. In the example shown in FIG. 1, the cable 76 has a first and second branched cables 76a and 76b that are bifurcated. The first and second branch cables 76a and 76b are connected to the first and second sheet-shaped electrodes 72a and 72b, respectively. The cable 76 has a common pin jack or pin plug 76c at the other end for connecting to the current generating unit 70.

In another embodiment, the conductive material is a single sheet-shaped electrode including a first electrode portion, a second electrode portion, and an insulating portion provided between the first and second electrode portions. The one sheet-shaped electrode has first and second terminals electrically connected to each of the first electrode portion and the second electrode portion, and the first and second terminals are provided. The terminal 2 can be connected to the current generator and the pulsed current can be applied to the skin. An example thereof is shown in FIG.
In FIG. 2, the conductive material has a single sheet-like electrode 82. The sheet-shaped electrode 82 includes a first electrode portion 82a and a second electrode portion 82b, and an insulating portion 82c is provided between them. The first and second electrode portions 82a and 82b and the insulating portion 82c are formed as a single sheet-like member. The sheet-shaped electrode 82 has first and second electrode- side terminals 84a and 84b connected to the first and second electrode portions 82a and 82b. The current generating unit 80 includes first and second power supply side terminals (not shown) that can be hooked or snapped to and separated from the first and second electrode side terminals 84a and 84b. By connecting the first and second electrode side terminals 84a and 84b to the first and second electrode side terminals, the sheet-shaped electrode 82 is connected to the current generation unit 80 without using a cable. At this time, the current generating unit 80 is attached to the sheet-shaped electrode 82.

The material of the conductive material is arbitrary, such as a conductive polymer having flexibility so as to be able to follow the unevenness of the skin, carbon black, resin, a dielectric elastomer actuator (DEA), an IPMC actuator, a PVC gel, and the like. Further, it may be thin so that it can be attached to the user's skin and transparent so that the wearing is not conspicuous, or it may be an embodiment matching the color of the user's skin. The conductive material may be reusable or disposable, and a make-up or sunscreen may be applied over it.

The conductive material itself may have adhesiveness, and an adhesive layer for attaching the pulse wave having the frequency of the present invention to the skin of the user may be provided to the extent that the pulse wave can be applied to the skin. Further, the attachment of the conductive material to the skin may be achieved by providing an adhesive layer on the device portion or by providing the device with a mounting portion such as a belt, or may be achieved by the adhesiveness of the sheet electrode. A chemical layer may be arranged between the conductive layer and the adhesive layer. Alternatively, in addition to the conductive layer and the adhesive layer 56, a microneedle layer having microneedles made of hyaluronic acid or the like can be included. The microneedle layer can be provided on the adhesive layer on the opposite side of the conductive layer, that is, on the surface in contact with the user's skin.

The adhesive layer used for the electrode may be any material as long as it has a holding force when it adheres to the skin and does not cause pain when peeled off, and is applied, impregnated, adhered, or cast on one surface of the support material. It is provided by means such as. Preferred examples of the adhesive component include various water-soluble polymers, oil-soluble polymers, and gel agents obtained by partially modifying these by means such as cross-linking. The type of cross-linking agent, compounding agent, and compounding condition can be selected from known methods.

Examples of such water-soluble polymers include polyacrylic acid, polyacrylic acid salt, polymethacrylic acid, polymethacrylate, polyvinyl alcohol, polyvinylpyrrolidone, polyacrylamide, polyacrylamide salt, polymethacrylamide, polymethacrylate salt, and methacryloyl. Oxyethyltrimethylammonium chloride, methacrylamidepropyltrimethylammonium chloride, N-n-butoxymethylacrylamide, N-iso-butoxyacrylamide, t-butylacrylamide sulfonic acid, t-butylacrylamide sulfonate, dimethylaminopropylmethacrylamide, methoxyethylene anhydrous Examples thereof include succinic acid copolymers, dimethylaminoammonium chloride or copolymers of these polymerizable monomer raw materials and other polymerizable monomers, carboxymethyl cellulose salts, alginates, carrageenan, xanthan gum, agar or cationized guar gum.

Examples of the oil-soluble polymer include a pressure-sensitive adhesive composed of a vinyl acetate / crotonic acid copolymer, a vinyl propionate / crotonic acid copolymer, a vinyl acetate resin, a trimethylsiloxysilicate polymer, a silicon resin or an acrylic polymer. Rubber-based pressure-sensitive adhesives such as styrene-isoprene-styrene block copolymer, styrene-butadiene-styrene block copolymer, polyisoprene, poloisobutylene, and polybutadiene, silicone-based pressure-sensitive adhesives such as silicone rubber, dimethylsiloxane base, and diphenylsiloxane base. , Vinyl ether-based pressure-sensitive adhesives such as polyvinyl methyl ether, polyvinyl ethyl ether, polyvinyl isobutyl ether, vinyl ester-based pressure-sensitive adhesives such as vinyl acetate-ethylene copolymer, carboxylic acid components such as dimethyl terephthalate, dimethyl isophthalate, and dimethyl phthalate, and ethylene. Polyester-based pressure-sensitive adhesive composed of polyhydric alcohol components such as glycol, various rubber-based polymers, acrylic-modified silicon resin, acrylic-modified silicon resin, epoxy resin, polyacrylonitrile, or co-polymerization of these polymerizable monomer raw materials with other polymerizable monomers. Examples include polymers.

The material for forming the microneedles that can be applied to the adhesive layer may be any material that exhibits a certain degree of hardness so as to pierce the skin and cause irritation, and the material itself is not particularly limited. However, since it is applied to the skin for a long time, it needs to be a biocompatible material, and is preferably formed of a material that can be dissolved or swollen in the living body. Examples of such a material include polysaccharides such as hyaluronic acid, chitosan, maltose, arginate, amylose and agarose, celluloses such as carboxymethyl cellulose and hydroxypropyl cellulose, starch and the like, which are formed by each material alone. However, it may be formed from a mixture in which two or more kinds of materials are appropriately blended. A particularly preferred material is hyaluronic acid. Alternatively, it may be formed of a resin that does not dissolve or swell in the living body and does not affect the living body. For example, although it is not used as a material, polymethylmethacrylate, cellulose acetate, ethyl cellulose, polyethylene resin, polypropylene resin, ethylene. -Propylene copolymer, ethylene-vinyl acetate copolymer, vinyl chloride resin, vinylidene chloride resin, vinyl acetate-vinyl chloride copolymer, polyamide resin, polyester resin, ABS resin, SIS resin, SEBS resin, urethane resin , Silicon resin, aluminum and the like.

The drug layer may be arranged between the conductive layer and the adhesive layer or on the surface of the adhesive layer, or the adhesive layer or the microneedle itself may contain the drug. The drug is, for example, a water-soluble drug and a component of cosmetics conventionally used as a transdermal absorption preparation, for example, ascorbyl palmitate, kodiic acid, lucinol, tranexamic acid, potassium 4-methoxysalicylate, oily licorice extract, vitamin. Whitening ingredients such as A derivatives; Anti-wrinkle ingredients such as retinol, retinoic acid, retinol acetate, retinol palmitate; Blood circulation promoting ingredients such as tocopherol acetate, capsine, and vanillylamide norylate; Diet ingredients such as raspberry ketone, evening primrose extract, and seaweed extract; Antibacterial components such as isopropylmethylphenol, photosensitizer, and zinc oxide; may be medicinal components such as vitamins such as vitamin D2, vitamin D3, and vitamin K.

One aspect of the present invention relates to a beauty device. Since the beauty device of the present invention, preferably a wearable beauty device, can be used hands-free, it can be used in various scenes such as wearing while working. Moreover, since the beauty device of the present invention is small and light, it is easy to carry.

Hereinafter, the beauty device according to the preferred embodiment of the present invention will be described with reference to the accompanying drawings.
The beauty device 100 includes a device main body 10 and a sheet electrode 50. In the beauty device 100, the sheet electrode 50 forming the sheet portion is detachably attached to the surface of the user's skin S, and the device main body 10 as the device portion comes into contact with both the user's skin S and the sheet electrode 50. It is designed to be arranged and used like this. That is, the beauty device 100 constitutes a beauty device used by being attached to the surface of the human body, particularly the skin S.

In the present embodiment, the apparatus main body 10 includes a housing main body portion 12 and a lid closing portion 14 that form a hollow housing. The lid closing portion 14 is detachably attached to the housing main body portion 12. The housing main body portion 12 is a bottomed hollow member having one end opened and the other end closed by a bottom wall 12a. In the present embodiment, both the housing main body portion 12 and the lid closing portion 14 are cylindrical, but in another preferred embodiment, the housing main body portion 12 has a disk-shaped bottom wall and a lid closing portion as shown in FIG. 14 may constitute a housing with, for example, a dome-shaped closure coupled along the outer peripheral edge of the bottom wall. The shape of the closed lid is not particularly limited and can be arbitrarily changed depending on the mounting site and form. For example, it may be a dome shape, a circle, an ellipse, a triangle, a square, a rectangle, or a polygon, and is curved according to the mounting site. You may be. In the housings 12 and 14, a circuit board 20 constituting a pulse wave generation unit for generating a pulse wave and a control unit for controlling the pulse wave generation unit is arranged. The control unit controls the frequency, waveform, amplitude, etc. of the pulse wave of the pulse wave generation unit. In the housings 12 and 14, a battery 28 is further housed as a power source for supplying electric power to the pulse wave generation unit and the control unit. The battery 28 can be a rechargeable rechargeable battery.

The device main body 10 also has a switch 22 for turning on / off the device main body 10, particularly the pulse wave generation unit on the circuit board 20. A part of the switch 22 protrudes from the side surface of the housing main body portion 12 or the lid closing portion 14, and is pushed in in the radial direction to perform an on / off operation. In another preferred embodiment, as shown in FIG. 13, the switch 22 may be provided on the upper part of the dome-shaped closing portion 14 and the switch may be pushed downward to perform an on / off operation. When the battery 28 is a rechargeable battery, the apparatus main body 10 can include terminals 24 and 26 for connecting a charger (not shown) for charging the battery 28. The terminals 24 and 26 are arranged so that the cord of the charger (not shown) can be connected from the side surface of the housing main body portion 12 or the lid closing portion 14. In the present embodiment, the terminals 24 and 26 are separate types in which the positive pole and the negative pole are separated, but may be a coaxial type terminal having a positive pole and a negative pole. Alternatively, the charging terminal may be a terminal for connecting a Type-A or Type-C USB cable, a Lightning (registered trademark) cable, or the like.

The circuit board 20 can also include a wireless communication port (not shown) capable of wireless communication with an external operating device. In this case, the control unit can control the frequency and voltage of the pulse wave generated by the transmitter based on the signal from the operating device. As an example, the operating device can be formed by a dedicated wireless operating device (not shown). In this case, the operating device can be wirelessly connected to the wireless communication port by analog or digital communication of a specific frequency. Alternatively, the operating device may be a commercially available handheld device such as a smartphone or tablet. In this case, the operation application can be installed on the handheld device, and the operation device can be wirelessly connected to the wireless communication port via WIFI or Bluetooth (registered trademark).

In one embodiment, a data receiving unit is provided instead of the switch 22, and the control unit may be remotely operated wirelessly by, for example, an operating device that uses data stored in a mobile phone, cloud, or the like. ..

The first and second electrodes 16 and 18 are arranged on the outer side of the housing of the bottom wall 12a of the housing main body portion 12. In the circuit board 20, the first and second output terminals 30 and 32 connected to the first and second electrodes 16 and 18 are provided on the inner side of the housing of the bottom wall 12a. In the present embodiment, the circular first electrode 16 is a sheet connecting electrode for contacting the sheet electrode 50 and electrically connecting the pulse wave generating portion and the sheet electrode 50. A substantially semicircular second electrode 18 larger than the electrode 16 serves as a skin electrode for contacting the surface of the user's skin S and electrically connecting the pulse wave generating portion and the skin S. ing. Here, the shapes of the first and second electrodes are arbitrary as long as the desired pulse wave can reach the skin S.

The sheet electrode 50 is made of a conductive material that applies a pulse wave from a pulse wave generating portion to the skin S via a first electrode 16 which is a sheet connecting electrode. The material of the sheet electrode 50 is arbitrary, such as a conductive polymer having flexibility so as to be able to follow the unevenness of the skin, carbon black, resin, a dielectric elastomer actuator (DEA), an IPMC actuator, a PVC gel, and the like. A first portion 50a, which is a main part to be attached to the surface of the user's skin S and a pulse wave is applied to the skin S, and a second portion, which is a protruding portion extending outward from the first portion 50a. It has a portion 50b of. The first portion 50a can have an area sufficient to effectively and effectively promote collagen production for the skin S in the desired region when the pulse wave is applied to the skin S of the user. The sheet electrode 50 can take any shape. As shown in FIG. 1, the first portion 50a may have a circular shape, or may match the shape of a desired sticking portion such as a cheek. The shape of the second portion 50b is also arbitrary as long as it has a sufficient length so that the apparatus main body 10 can be arranged apart from the first portion 50a.

The sheet electrode 50 may be thin so that it can be attached to the user's skin, and may be transparent so that the wearing is not conspicuous, or may be an embodiment that matches the color of the user's skin. The sheet electrode 50 may be used repeatedly or may be disposable, and it may be possible to apply make-up or a sunscreen agent on the sheet electrode.

In one embodiment, the sheet electrode 50 itself may have adhesiveness, and the sheet electrode 50 may be adhered to the user's skin S to the extent that the pulse wave having the frequency of the present invention can be applied to the skin. A layer may be provided. Further, the attachment of the apparatus main body 10s to the skin may be achieved by providing an adhesive layer on the device portion or by providing the device with a mounting portion such as a belt, or may be achieved by the adhesiveness of the sheet electrode.

Next, the configuration of the sheet electrode 50 will be described with reference to FIGS. 8 to 10.
Referring to FIG. 8, the sheet electrode 50 can have a laminated structure composed of three layers as an example. The sheet electrode 50 can have, for example, a flexible and stretchable stretchable layer 52, a conductive layer 54, and an adhesive layer 56. The elastic layer 52 can be formed from, for example, a non-woven fabric. The conductive layer 54 can be formed of, for example, a conductive polymer, carbon black, resin, a dielectric elastomer actuator (DEA), an IPMC actuator, a PVC gel, or the like.

Further, the sheet electrode 50 may have four layers. As shown in FIG. 9, the sheet electrode 50 can include a drug layer 58 arranged between the conductive layer 54 and the adhesive layer 56 in addition to the elastic layer 52, the conductive layer 54 and the adhesive layer 56. Alternatively, the sheet electrode 50 can include a microneedle layer 60 having microneedles made of hyaluronic acid or the like, in addition to the elastic layer 52, the conductive layer 54 and the adhesive layer 56. The microneedle layer 60 can be provided on the opposite side of the conductive layer 64 in the adhesive layer 56, that is, on the surface in contact with the user's skin when the sheet electrode 50 is attached to the user's skin.

When using the beauty device 100, first, the sheet electrode 50 is attached to a desired position on the user's skin S. At that time, the orientation of the second portion 50b is determined so that the apparatus main body 10 can be arranged at an appropriate position on the surface of the user's skin S. Next, the bottom wall 12a of the housing main body 12 is made to face the user's skin S, and as shown in FIG. 11, the first electrode 16 is brought into contact with the second portion 50b of the sheet electrode 50, and the second electrode is used. The device body 10 is attached to the surface of the user's skin S by arranging the 18 so as to be in contact with the user's skin S. Next, the switch 22 is turned on to apply a pulse wave to the user's skin S. As an example of the pulse wave, it may be a pulse-shaped pulse wave as shown in FIG. 120. However, the pulse wave waveform shown in FIG. 12 is an example, and the present invention is not limited to this, and a square wave, a sine wave, a triangular wave, a sawtooth wave, or the like can be arbitrarily set.

According to the present embodiment, when the beauty device 100 is attached to the user's skin S, the first electrode 16 and the sheet electrode 50, which are skin electrodes, come into contact with the user's skin S, and the apparatus main body 10 is operated. When the pulse wave is applied to the skin S from the pulse wave generation portion, the first electrode 16 and the sheet electrode 50 are electrically conducted through the user's skin S, and the pulse wave is applied to the user's skin S. By doing so, in the skin S in contact with the first portion 50a of the sheet electrode 50, which is the mounting site, collagen production is promoted, moisturization is promoted, and wrinkles are improved.

The beauty device of the present invention is provided with additional functions such as ion introduction function, ion derivation function, EMS, sound wave vibration, radio wave generation function, LED generation function, heating function, cooling function and the like, if necessary. May be good. In addition, the cosmetology device / method of the present invention may be used in combination with other treatments. Other treatments include, but are not limited to, application of various stimuli such as the above-mentioned light stimulus and physical stimulus, application of stimulus by human hand, application of cosmetics, and the like.

The effect of the skin electrode portion of the present invention is remarkable even if it is directly attached to the skin. However, in order to further enhance the beauty effect, any cosmetics such as gel, cream, lotion, hyaluronic acid, collagen, vitamins, and moisturizers that promote the beauty effect are used in combination with the skin, and the present invention is applied on the skin. You may apply the beauty equipment by wearing the sheet.

The method of the present invention is intended for beauty and may not be a treatment method used by doctors and medical professionals. The present invention also provides a cosmetological counseling method that supports a cosmetological act of the subject, including presenting the cosmetological method / device of the present invention to the subject.

All documents referred to herein are incorporated herein by reference in their entirety.

The examples of the present invention described below are for illustration purposes only and do not limit the technical scope of the present invention. The technical scope of the invention is limited only by the description of the claims. Modifications of the present invention, for example, addition, deletion and replacement of the constituent elements of the present invention may be made on condition that the gist of the present invention is not deviated.

Hereinafter, the present invention will be described in more detail based on examples, but these are not intended to limit the present invention in any way.

<Example 1>
<Collagen production promoting action by electrical stimulation>
METHODS: A cell reactor was prepared by the method described in N. Tandon et al., Nat. Protocol. 4 (2009) 155-173. A resistance of 1 kΩ was applied to human fibroblasts (Normal Human Thermal Fiberblast manufactured by ATCC in the United States) in a cell reactor using a voltage divider, and a pulse wave generating device was used to generate 1 Hz, 500 Hz, 2000 Hz pulse waves (5 V, 5 μA). The stimulus was given for 2 hours. FIG. 120 shows a waveform at 2000 Hz. Even in the case of 1 Hz and 500 Hz, they have the same pulse-like waveform except that only the frequency is different.
The amount of collagen in fibroblasts was measured as follows using a Sircol ™ soluble collagen assay kit (Biocolor Ltd.).
1.0 ml of Sircol ™ dye reagent is added to a tube containing 100 μl of the above fibroblast supernatant. Cover the tube and mix manually at 5 minute intervals for a total of 30 minutes. The tube is then transferred to a microcentrifuge to remove unbound dye by suction. After adding 750 μl of ice-cold acid-salt wash reagent, transfer the tube to a microcentrifuge and centrifuge at 12,000 rpm for 10 minutes. The acid-salt cleaning reagent is removed by suction and 250 μl of alkaline reagent is added to each tube. The collagen-binding pigment is released into the solution using a vortex mixture. Then, 200 μl of the solution in each tube is transferred to the wells of the 96-well plate. Read at 555 nm using a plate reader (Thermo Electron Corporation). The quantification is performed by triplicate, and the case where no electrical stimulation is applied to the cells is used as a control and contrasted. The concentration of collagen produced is determined by contrasting with the calibration curve and standardized by the number of cells lysed by the assay.

The results are shown in FIG. When the frequencies were 500 Hz and 2000 Hz, high collagen production promoting effects were observed, about 2.0 times and about 6.0 times, respectively, as compared with the subject. In particular, remarkable collagen production is observed when the frequency is 2000 Hz, and it can be seen that the higher the frequency, the more the collagen production is promoted. The apparatus of the present invention has the effect of promoting collagen production in skin cells simply by applying a skin electrode to the skin and applying a pulse wave at a high frequency.

<Example 2>
<Moisturizing effect by electrical stimulation>
Subject: 10 healthy men in their 40s and 60s who have no skin problems Equipment used:
-Device of the present invention (self-made)
・ Omron skin gel pad (conductive electrode (carbon black) adhesive gel, model number: HVLLPAD) 2 cm x 2 cm
-Courage + Khazaka Corneometer (model number: CM825)
Method: The experiment was performed at a temperature of 24 ° C. and a humidity of 45%. After washing the skin with soap and water to remove water, the skin water content was measured with a corneometer. The gel pad was attached to the skin. The gel pad was connected to the device of the present invention, and the skin was subjected to electrical stimulation of a pulse wave of 5 V, 250 μA, 500 Hz for 20 minutes. The pad was removed from the skin, and at 15, 30, 45, and 60 minutes, the water content of the skin was measured over time with a corneometer. As a control, the water content was also measured when the cosmetic was applied for the same time without the pulse wave and when neither the pulse wave nor the cosmetic was given.

The result of skin water content is shown in FIG. It can be seen that after 20 minutes of application, a water content of 20 au or more is maintained for 60 minutes or more after application. The effect was remarkable even when compared with the control using only the cosmetics. The device of the present invention can increase the water content of the skin and moisturize the skin simply by applying a skin electrode to the skin and applying a pulse wave at a high frequency.

<Example 3>
<Wrinkle improvement effect by pulse wave stimulation>
The same electrical stimulation was applied to the same subjects as in Example 2 except that wrinkles were measured by the following method instead of water content measurement, and the wrinkle improvement effect was investigated. Skin wrinkles were imaged using an ANTERA 3D camera for skin analysis.

The results are shown in FIG. The figure below shows an actual photograph of the skin taken with an ANTERA 3D camera, and the figure above is an image of the surface morphology of the part of the skin corresponding to the figure below. It can be seen that the wrinkle improving effect is maintained even after 7 hours have passed and 48 hours have passed since the stimulus was applied for 20 minutes. The device of the present invention has an effect of improving wrinkles on the skin simply by applying a skin electrode to the skin and applying a pulse wave at a high frequency.

<Example 4>
<Wrinkle improving effect by multiple electrical stimuli>
The same subjects as in Examples 2 and 3 were given the same 20-minute electrical stimulation except that the electrical stimulation was applied three times after 0 hour, 24 hours, and 48 hours. Skin wrinkles were imaged using an ANTERA 3D camera and the wrinkle depth was quantified by determining the Indentation Index (μm) using Miravex 1 software.

The result of wrinkle measurement is shown in FIG. It decreased by about 6.0 by the first stimulus, but then returned to the original level, but decreased by about 2.0 by the second stimulus and further decreased by the third stimulus. It was found that the wrinkle improving effect was further enhanced by applying a plurality of stimuli in this way as compared with a single stimulus.

From the above results, it was found that the beauty device of the present invention has the effects of promoting collagen production in the skin, moisturizing, and improving wrinkles. When the collagen production of the skin is promoted, moisturized, and wrinkles are improved, it is effective for the elasticity and elasticity of the skin.

<Example 5>
For the purpose of demonstrating that the device of the present invention exerts an excellent cosmetic effect, the effect was compared with a commercially available facial device. As a commercially available facial device, another company's product was used. The device activates the skin by passing a weak electric current through the skin. The wrinkle reducing effect was investigated by determining the indentation index (μm) and quantifying the wrinkle depth in the same manner as in Example 4.
The result is shown in FIG.
“Before application” in measurement 1 on the left side of the figure indicates the dent index of the application site immediately before application by another company's product.
“Gel application” in measurement 2 indicates the dent index of the skin area to which only gel application is applied. When using other companies' products, it is necessary to apply gel to the application area before applying current stimulation. Therefore, in order to eliminate the beauty effect of the gel application itself, the dent index in which only the gel application was applied was measured.
“After application” in measurement 3 indicates the dent index at the location where the gel was applied and the current stimulus was applied. The current stimulation was performed by giving the current stimulation to the skin for 5 minutes according to the instruction manual of the product of another company.
Since there is no difference in the dent index between measurements 2 and 3, it is considered that the effect seen by applying the products of other companies is not the effect of electrical stimulation but the effect of gel application. Therefore, it can be seen that the wrinkle reduction effect of the current stimulation of other companies' products cannot be expected so much if it is limited to the effect using the dent index as an index.
The right side of FIG. 18 shows the amount of change in the depression index with respect to “before application” of measurement 1. The amount of change "after application" is the value obtained by subtracting the amount of change in <gel application only> from the amount of change in <gel application + current stimulation>, and the effect of using the dent index as an index is the current stimulation by other companies' products. It is clear from this figure that we cannot expect it.

A direct comparison test was conducted between the apparatus of the present invention and a product of another company under the same environment. The result is shown in FIG. 197.
"Before application" on the left side of the figure shows the dent index of the part where only the gel is applied in the case of another company's product, and in the case of the present invention, shows the dent index of the skin in the state where no treatment is applied.
“After application” indicates the dent index of the portion where the gel is applied and the current stimulus is applied in the case of another company's product, and in the case of the present invention, the dent index measured after applying a 0.5 kHz pulse for 20 minutes is indicated. In the case of the device of the present invention, no cosmetics such as gel is used. The right side of FIG. 19 shows the amount of change in the depression index with respect to “before application”.
From both figures, the wrinkle improvement effect using the dent index as an index cannot be expected with the current stimulation by the products of other companies, but the clear effect was confirmed by the apparatus of the present invention.

The embodiment of the present invention has been described above. However, the present invention is not limited to these, and the members, materials thereof, and the like can be appropriately changed without departing from the spirit of the invention.

10 Device body 12 Housing body 12a Bottom wall 14 Closed lid 16 First electrode 18 Second electrode 20 Circuit board 22 Switch 24 terminal 26 terminal 28 Battery 30 First output terminal 32 Second output terminal 50 Sheet electrode 50a 1st part 50b 2nd part 100 Beauty equipment 61 Controller 62 Conductive gel 64 Skin 71 Controller 72 Conductive gel 73 Insulation part 74 Skin

Claims (16)

1. A cosmetological method for promoting collagen production in the skin, including applying a pulse current to the target skin.
2. The beauty method according to claim 1, wherein the pulse current has a frequency in the range of 200 Hz to 5000 Hz.
3. The beauty method according to claim 2, wherein the current value of the pulse current is in the range of 100 to 1000 μA.
4. The beauty method according to any one of claims 1 to 3, wherein a pulse current is applied to the target skin via a conductive material.
5. The conductive material is arranged between the flexible base layer, the adhesive layer attached to one side surface of the base layer and having adhesiveness to the user's skin, and the base layer and the adhesive layer. The beauty method according to claim 4, wherein the conductive layer is composed of a conductive layer, and the conductive layer is electrically coupled to an electrode that applies a current from a current generating portion.
6. The beauty method according to claim 5, wherein a drug layer is further arranged between the conductive layer and the adhesive layer or on the surface of the adhesive layer.
7. The beauty method according to claim 5 or 6, wherein the adhesive layer contains a drug.
8. The beauty method according to any one of claims 5 to 7, wherein microneedles are applied to the surface of the adhesive layer.
9. The beauty method according to any one of claims 1 to 8 for promoting skin moisturization.
10. The beauty method according to any one of claims 1 to 9 for improving skin wrinkles.
11. The beauty method according to any one of claims 1 to 10, wherein the step of applying the pulse current to the target skin is carried out for 1 to 120 minutes.
12. 5. The conductive material has first and second sheet-shaped electrodes that are independent of each other, and each of the first and second sheet-shaped electrodes is connected to a current generating portion through a conductive cable. The beauty method according to any one of 11 to 11.
13. The conductive material comprises one sheet-like electrode including a first electrode portion, a second electrode portion, and an insulating portion provided between the first and second electrode portions. The sheet-shaped electrode has first and second terminals electrically connected to each of the first electrode portion and the second electrode portion, and the first and second terminals are currented. The beauty method according to any one of claims 5 to 11, which is connected to the generator.
14. The beauty method is carried out by using a beauty device, and the beauty device is
    It is equipped with a device unit and a sheet-shaped skin electrode that contacts the user's skin and gives electrical stimulation.
    The device unit is a current generation unit for generating a pulse current, a control unit for controlling the current generation unit, and a skin electrode connection electrode for electrically connecting the current generation unit and the skin electrode. And a ground electrode for electrically connecting between the current generating part and the skin,
    The skin electrode is made of a conductive material that applies the current from the current generating part to the skin via the sheet connection electrode.
    When the beauty device is attached to the user's skin, the ground electrode and skin electrode come into contact with the user's skin, and when a pulse current is generated from the power generation unit by operating the control unit, the ground electrode and skin electrode 1. The beauty method described in item 1.
15. Further, a hollow housing for accommodating the current generation unit, the control unit, the current generation unit, and the power supply for supplying electric power to the control unit is provided.
    The hollow housing has a flat plate-shaped bottom wall, and the sheet connecting electrode and the ground electrode are arranged on the outer surface of the bottom wall.
    The beauty method according to claim 14, wherein the sheet connection electrode and the ground electrode are brought into direct contact with the skin electrode and the user's skin when attached to the user.
16. The cosmetological method of claim 15, wherein the hollow housing comprises a bottom wall and a closure coupled along an outer peripheral edge of the bottom wall.

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