WO2022117740A3 - Genetically modified streptomyces bacteria - Google Patents

Genetically modified streptomyces bacteria Download PDF

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Publication number
WO2022117740A3
WO2022117740A3 PCT/EP2021/084001 EP2021084001W WO2022117740A3 WO 2022117740 A3 WO2022117740 A3 WO 2022117740A3 EP 2021084001 W EP2021084001 W EP 2021084001W WO 2022117740 A3 WO2022117740 A3 WO 2022117740A3
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WO
WIPO (PCT)
Prior art keywords
genetically modified
streptomyces bacteria
modified streptomyces
bacterium
bacteria
Prior art date
Application number
PCT/EP2021/084001
Other languages
French (fr)
Other versions
WO2022117740A2 (en
Inventor
Lucie CREPIN
Caroline SCHIAVON
Original Assignee
Pili
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pili filed Critical Pili
Publication of WO2022117740A2 publication Critical patent/WO2022117740A2/en
Publication of WO2022117740A3 publication Critical patent/WO2022117740A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P15/00—Preparation of compounds containing at least three condensed carbocyclic rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to recombinant Streptomyces bacterium wherein said bacterium has been genetically modified to delete a part of the actinorhodin gene cluster. It further relates to the use of said recombinant Streptomyces bacterium to produce 3,8- dihydroxy-l-methyl-anthraquinone-3-carboxylic acid and aloesaponarin II.
PCT/EP2021/084001 2020-12-03 2021-12-02 Genetically modified streptomyces bacteria WO2022117740A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP20306494 2020-12-03
EP20306494.4 2020-12-03

Publications (2)

Publication Number Publication Date
WO2022117740A2 WO2022117740A2 (en) 2022-06-09
WO2022117740A3 true WO2022117740A3 (en) 2022-07-14

Family

ID=73856523

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2021/084001 WO2022117740A2 (en) 2020-12-03 2021-12-02 Genetically modified streptomyces bacteria

Country Status (2)

Country Link
TW (1) TW202237817A (en)
WO (1) WO2022117740A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1568774A2 (en) * 1993-09-20 2005-08-31 The Leland Stanford Junior University Recombinant production of novel polyketides

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1568774A2 (en) * 1993-09-20 2005-08-31 The Leland Stanford Junior University Recombinant production of novel polyketides

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BARTEL P L ET AL: "Biosynthesis of anthraquinones by interspecies cloning of actinorhodin biosynthesis genes in streptomycetes: clarification of actinorhodin gene functions", JOURNAL OF BACTERIOLOGY (PRINT), AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 172, no. 9, 1 September 1990 (1990-09-01), pages 4816 - 4826, XP002332286, ISSN: 0021-9193 *
LU Z. ET AL: "Promotion of markerless deletion of the actinorhodin biosynthetic gene cluster in Streptomyces coelicolor", ACTA BIOCHIMICA ET BIOPHYSICA SINICA, vol. 42, no. 10, 1 October 2010 (2010-10-01), US, pages 717 - 721, XP055804911, ISSN: 1672-9145, DOI: 10.1093/abbs/gmq080 *

Also Published As

Publication number Publication date
TW202237817A (en) 2022-10-01
WO2022117740A2 (en) 2022-06-09

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