WO2022117695A1 - Système et procédé d'imagerie optique à super-résolution - Google Patents
Système et procédé d'imagerie optique à super-résolution Download PDFInfo
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- WO2022117695A1 WO2022117695A1 PCT/EP2021/083882 EP2021083882W WO2022117695A1 WO 2022117695 A1 WO2022117695 A1 WO 2022117695A1 EP 2021083882 W EP2021083882 W EP 2021083882W WO 2022117695 A1 WO2022117695 A1 WO 2022117695A1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Optics & Photonics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
L'invention concerne un système d'imagerie (1) et un procédé d'imagerie à super-résolution de champ lointain microscopique ayant une résolution optique, le système d'imagerie (1) comprenant au moins les composants suivants : - Une source de lumière d'excitation (2) configurée pour fournir une lumière d'excitation à l'intérieur d'une plage de longueurs d'onde d'excitation prédéfinie, - un premier objectif (3) agencé et configuré pour éclairer un échantillon (4) dans un espace d'échantillon (SP) du système d'imagerie (1), l'échantillon (4) comprenant des émetteurs (506) qui sont excitables avec la lumière d'excitation et qui émettent une lumière d'émission lors de l'excitation avec une lumière d'excitation, - un support d'échantillon (5) agencé et configuré pour supporter l'échantillon (4) dans l'espace d'échantillon (SP), le porte-échantillon (5) comprenant une première fenêtre transparente (6) pour supporter l'échantillon (4), le porte-échantillon (5) étant agencé de telle sorte que la lumière d'excitation provenant du premier objectif (3) se propage à travers la première fenêtre (6) du porte-échantillon (6) avant d'atteindre l'espace d'échantillon (SP), - une optique de détection de champ lointain (3, 7) configuré et agencé de telle sorte que, dans le système, une lumière d'émission est collectée à partir de l'espace d'échantillon (SP) et se propage vers un espace d'image le long d'un trajet de détection, - un détecteur (9) disposé dans l'espace d'image et configuré pour enregistrer la lumière d'émission provenant de l'échantillon (4), - une suspension colloïdale (10, 503) comprenant des particules (11, 502), les particules (11, 502) présentant des propriétés de diffusion de la lumière, d'absorption et de plasmonique, la suspension (10, 503) étant disposée dans l'espace d'échantillon (SP) ou entre la première fenêtre (6) du porte-échantillon (5) et le premier objectif (3), de telle sorte que l'excitation des émetteurs (506) avec une lumière d'excitation varie de manière aléatoire à la fois dans l'espace et dans le temps aux positions des émetteurs (506), en raison des propriétés de diffusion, d'absorption et de plasmonique des particules (11, 502) subissant un mouvement brownien dans la suspension (10, 503), de telle sorte que la lumière émise par les émetteurs (506) varie de manière aléatoire.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP20211355 | 2020-12-02 | ||
| EP20211355.1 | 2020-12-02 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2022117695A1 true WO2022117695A1 (fr) | 2022-06-09 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/EP2021/083882 WO2022117695A1 (fr) | 2020-12-02 | 2021-12-02 | Système et procédé d'imagerie optique à super-résolution |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO2022117695A1 (fr) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090116024A1 (en) * | 2006-04-07 | 2009-05-07 | Limo Patentverwaltung Gmbh & Co. Kg | Method for obtaining a high resolution image |
-
2021
- 2021-12-02 WO PCT/EP2021/083882 patent/WO2022117695A1/fr active Application Filing
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090116024A1 (en) * | 2006-04-07 | 2009-05-07 | Limo Patentverwaltung Gmbh & Co. Kg | Method for obtaining a high resolution image |
Non-Patent Citations (14)
| Title |
|---|
| A. LIUTKUSD. MARTINAS. POPOFFG. CHARDONO. KATZG. LEROSEYS. GIGANL. DAUDETI. CARRON: "Imaging With Nature: Compressive Imaging Using a Multiply Scattering Medium", SCI. REP., vol. 4, 2014, pages 5552 |
| B. O'DONOGHUEE. CHUN. PARIKHS. BOYD: "Conic Optimization via Operator Splitting and Homogeneous Self-Dual Embedding", J. OPTIM. THEORY APPL., 2016 |
| BABCOCK HAZEN P. ET AL: "Fast compressed sensing analysis for super-resolution imaging using L1-homotopy", OPTICS EXPRESS, vol. 21, no. 23, 13 November 2013 (2013-11-13), pages 28583, XP055801314, DOI: 10.1364/OE.21.028583 * |
| F. HUANGT. M. P. HARTWICHF. E. RIVERA-MOLINAY. LINW. C. DUIMJ. J. LONGP. D. UCHILJ. R. MYERSM. A. BAIRDW. MOTHES: "Video-rate nanoscopy using sCMOS camera-specific single-molecule localization algorithms", NAT. METHODS., 2013, pages 10 |
| I. RISHG. GRABARNIK: "2009 47th Annual Allerton Conference on Communication, Control, and Computing (Allerton", 2009, IEEE |
| ILOVITSH TALI ET AL: "Superresolved nanoscopy using Brownian motion of fluorescently labeled gold nanoparticles", APPLIED OPTICS, vol. 56, no. 5, 6 February 2017 (2017-02-06), US, pages 1365, XP055800868, ISSN: 0003-6935, DOI: 10.1364/AO.56.001365 * |
| J. J. SCHMIEDA. GIETLP. HOLZMEISTERC. FORTHMANNC. STEINHAUERT. DAMMEYERP. TINNEFELD: "Fluorescence and super-resolution standards based on DNA origami", NAT. METHODS., vol. 9, 2012, pages 1133 - 1134, XP037547643, DOI: 10.1038/nmeth.2254 |
| J. J. SCHMIEDM. RAABC. FORTHMANNE. PIBIRIB. WUNSCHT. DAMMEYERP. TINNEFELD: "DNA origami-based standards for quantitative fluorescence microscopy", NAT. PROTOC., vol. 9, 2014, pages 1367 - 1391 |
| J. LOFBERG, PROCEEDINGS OF THE IEEE INTERNATIONAL SYMPOSIUM ON COMPUTER-AIDED CONTROL SYSTEM DESIGN, 2004 |
| MIKLYAEV YU V ET AL: "Superresolution microscopy in far-field by near-field optical random mapping nanoscopy", APPLIED PHYSICS LETTERS, A I P PUBLISHING LLC, US, vol. 105, no. 11, 15 September 2014 (2014-09-15), XP012189908, ISSN: 0003-6951, [retrieved on 19010101], DOI: 10.1063/1.4895922 * |
| R. DIEKMANNK. TILLM. MULLERM. SIMONISM. SCHUTTPELZT. HUSER: "Characterization of an industry-grade CMOS camera well suited for single molecule localization microscopy - high performance super-resolution at low cost", SCI. REP., vol. 7, 2017, pages 14425, XP055525087, DOI: 10.1038/s41598-017-14762-6 |
| S. CULLEYD. ALBRECHTC. JACOBSP. M. PEREIRAC. LETERRIERJ. MERCERR. HENRIQUES: "Quantitative mapping and minimization of super-resolution optical imaging artifacts", NAT. METHODS, 2018 |
| YANIC HEER: "Exploiting Sparsity in Super-resolution and Single Particle Tracking Microscopy", 1 January 2017, DISSERTATION, UNIVERSITÄT ZÜRICH, CH, PAGE(S) 1 - 92, XP009527909 * |
| Z. YANGC. ZHANGL. XIE: "Robustly Stable Signal Recovery in Compressed Sensing With Structured Matrix Perturbation", IEEE TRANS. SIGNAL PROCESS, vol. 60, 2012, pages 4658 - 4671 |
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