WO2022097018A1 - Composition de nettoyage et désinfectante écologique - Google Patents

Composition de nettoyage et désinfectante écologique Download PDF

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Publication number
WO2022097018A1
WO2022097018A1 PCT/IB2021/060121 IB2021060121W WO2022097018A1 WO 2022097018 A1 WO2022097018 A1 WO 2022097018A1 IB 2021060121 W IB2021060121 W IB 2021060121W WO 2022097018 A1 WO2022097018 A1 WO 2022097018A1
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WIPO (PCT)
Prior art keywords
cum
composition
disinfectant
cleaner
water
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PCT/IB2021/060121
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English (en)
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Chetana Umesh Virkud
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Chetana Umesh Virkud
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Publication of WO2022097018A1 publication Critical patent/WO2022097018A1/fr

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Classifications

    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/382Vegetable products, e.g. soya meal, wood flour, sawdust
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/58Meliaceae (Chinaberry or Mahogany family), e.g. Azadirachta (neem)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/77Sapindaceae (Soapberry family), e.g. lychee or soapberry
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/02Inorganic compounds ; Elemental compounds
    • C11D3/04Water-soluble compounds
    • C11D3/046Salts
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/50Perfumes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/16Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
    • A61L2/18Liquid substances or solutions comprising solids or dissolved gases

Definitions

  • the invention described herein relates to the field of naturally occurring ingredients mixed to form a composition which can be used as a cleaner cum disinfectant having antibacterial, antimicrobial and antifungal properties.
  • PBTs toxic chemicals
  • Disinfectants are chemical agents designed to inactivate or destroy microorganisms on inert surfaces. Disinfection does not necessarily kill all microorganisms, especially resistant bacterial spores; it is less effective than sterilization, which is an extreme physical or chemical process that kills all types of life.
  • Disinfectants work by destroying the cell wall of microbes or interfering with their metabolism. It is also a form of decontamination and can be defined as the process whereby physical or chemical methods are used to reduce the amount of pathogenic microorganisms on a surface.
  • Disinfectants kill more germs than sanitizers. Disinfectants are frequently used in hospitals, dental surgeries, kitchens, and bathrooms to kill infectious organisms. Sanitizers are mild compared to disinfectants and are used majorly to clean things which are in human contact whereas disinfectants are concentrated and are used to clean surfaces like floors and building premises etc. COVID-19 Impact on Global Surface Disinfectants
  • COVID-19 is an infectious disease caused by the most recently discovered novel coronavirus.
  • the World Health Organization (WHO) declared COVID-19 as a pandemic on March 11, 2020.
  • the pandemic has caused disruption in healthcare systems all over the world, with hospitals being overwhelmed due to the increasing influx of patients.
  • Temporary hospitals are also being set up to deal with increasing disease incidence.
  • the spread of COVID-19 has spurred a surge in the demand for cleaning and disinfection products due to the growing awareness about hygiene, health, and safety.
  • Increasing healthcare expenditure and an increase in the number of temporary hospitals are some of the major factors driving the demand and uptake of antiseptics and disinfectants.
  • the surface disinfectants market based on composition, is segmented into alcohols, chlorine compounds, quaternary ammonium compounds, hydrogen peroxide and other compositions.
  • the alcohols segment dominated the market in 2019.
  • the peracetic acid segment is expected to grow at a higher growth rate.
  • the large of the alcohols segment is attributed to the high use of alcohols on hard surfaces in hospitals and laboratories.
  • the surface disinfectants market is segmented into surface disinfection, instrument disinfection, and other applications.
  • the surface disinfection segment was projected to account for the largest share of the market.
  • the large share of this segment is attributed to the high prevalence of hospital-acquired infections across the globe and the higher volume of disinfectants required to clean these surfaces.
  • the end-users for surface disinfectants are hospital settings, diagnostic laboratories, research laboratories, and pharmaceutical & biotechnology companies.
  • a great many of the current antimicrobial compositions contain antimicrobial agents which are not naturally occurring.
  • Typical antimicrobial agents used in sanitizers and disinfectants include chemical disinfectants such as phenolic compounds, quaternary ammonium compounds, formaldehyde and halogen-containing compounds. Such materials are not of natural origin (i.e. not found in nature) and are prepared through chemical processing and synthesis. A great many of these “synthetic” disinfectants cause undesirable effects on both the environment and on human health.
  • additional chemical agents will generally have an effect on the pH and surface activity of the formulated product once in solution. It is well established that a number of simple disinfectants demonstrate their optimum activity at a specific pH (i.e. acidity or alkalinity). The ability of the disinfectant solution to make complete and even contact with the surface to be treated is also of great importance. This can generally be achieved by the addition of a surfactant or detergent to the formulation.
  • Disinfectants play a vital role in any biosecurity system, both in the process of terminal disinfection and in the ongoing hygiene maintenance. Apart from relatively minor changes and improvements in formulations, there has been little innovation in livestock disinfectant and large-surface disinfectant development for some fifteen to twenty years.
  • the toxicity of a specific substance depends on a variety of factors, including how much of the substance a person is exposed to, how they are exposed, and for how long.
  • Quaternary ammonium salts are used as disinfectants, surfactants, fabric softeners, and as antistatic agents (e.g. in shampoos). Quaternary ammonium compounds have also been shown to have antimicrobial activity. Certain quaternary ammonium compounds, especially those containing long alkyl chains, are used as antimicrobials and disinfectants. Examples are benzalkonium chloride, benzethonium chloride, methylbenzethonium chloride, etc.,
  • Quaternary ammonium compounds are believed to act by disrupting the cell membrane or viral envelope. Quaternary ammonium compounds are lethal to a wide variety of organisms except for endospores, Mycobacterium tuberculosis and non-enveloped viruses.
  • Quaternary ammonium compounds are cationic detergents, as well as disinfectants, and as such can be used to remove organic material.
  • Quaternary ammonium compounds can display a range of health effects, amongst which are mild skin and respiratory irritation up to severe caustic burns on the skin and the gastrointestinal wall (depending on concentration), gastrointestinal symptoms (e.g., nausea and vomiting), coma, convulsions, hypotension and death.
  • this substance causes severe skin burns and eye damage, may cause fire or explosion (a strong oxidiser), is harmful if swallowed and is harmful if inhaled. Also, it is observed that this substance is harmful to aquatic life with long-lasting effects and may cause respiratory irritation.
  • this invention has been arrived out of a need to find out the effective alternatives to these highly toxic, irritating and carcinogenic synthetic cleaners, disinfectants which are easily available in the market and for that the composition made up of natural ingredients has turn up this patented composition.
  • Plant-derived surfactants as an alternative to synthetic surfactants: surface and antioxidant activities by Lenka Tmakova, Stanislav Sekretar, Stefan Schmidt. Article in Chemical Papers - Slovak Academy of Sciences, January 2015 -
  • US Patent No. 8691292B discloses an aqueous disinfectant formulation comprising at least one phenolic compound of natural origin; a surfactant sufficient to form a solution or dispersion of the essential oil in an aqueous carrier; a solvent, and sufficient water.
  • the said invention relates to aqueous formulations suitable for disinfecting and cleaning large surfaces and comprising one or more phenolic compounds of natural origin.
  • US Patent No. 5403587A discloses an aqueous antimicrobial composition containing one or more essential oils which exhibit antimicrobial properties and which can be combined with a water carrier and a solubilizing or dispersing agent to form a solution or a dispersion of the essential oil in the water carrier, said essential oil exhibiting antimicrobial properties when incorporated in said water carrier which can be used as sanitizers, disinfectants and disinfectant cleaner wherein said solubilizing or dispersing agent comprises chemicals like ethanol and lauryl dimethyl amine oxide.
  • US4414128A discloses liquid detergent compositions, particularly for use as hard surface cleaners, comprising of synthetic chemicals like l%-20% surfactant, 0.5%-10% mono- or sesquiterpenes, and 0.5%- 10% of a polar solvent having a solubility in water of from 0.2% to 10%, preferably benzyl alcohol.
  • W02001084936A1 discloses an antimicrobial composition and for sanitizing and disinfecting surfaces including leather, wood, metal, plastic and fabric, comprises a mixture of essential oils capable of being dissolved or dispersed by a solvent in a water carrier.
  • the mixture of essential oils preferably comprises thyme, lemon grass, clove and eucalyptus and solvent comprises of ethanol.
  • the inventor claims that the product of the invention is completely natural and non-toxic to adults and children even if swallowed.
  • US 6010993A displays disinfecting compositions comprising a peroxygen bleach, glutaraldehyde, an antimicrobial essential oil and an amphoteric surfactant, preferably useful in combination with an amine oxide surfactant.
  • the compositions are especially for cleaning and disinfecting hard surfaces.
  • ES2437759T3 disclosed disinfectant formulation relates to new combinations of active ingredients that contain at least acertain substituted enamino carbonyl compound and azadirachtin and are very suitable for combating 5 animal parasites such as insects and unwanted saccharides.
  • An object of this invention is to make (provide) eco-friendly and economical composition using all the natural ingredients as a cleaner cum disinfectant having a low carbon footprint.
  • Another object of this invention is to provide antibacterial cleaner cum disinfectant compositions.
  • Another object of this invention is to provide safe cleaner cum disinfectant compositions, which do not cause side effects to human beings.
  • Another object of this invention is to provide cleaner cum disinfectant compositions, which do not accumulate in the human system.
  • Another object of this invention is to provide cleaner cum disinfectant compositions, which is biodegradable.
  • Another object of this invention is to provide cleaner cum disinfectant compositions, which do not cause any adverse effect on the surface.
  • Another object of this invention is to provide cleaner cum disinfectant compositions, which is easy to manufacture.
  • Another object of this invention is to provide cleaner cum disinfectant compositions, which are economical.
  • Another object of this invention is to provide cleaner cum disinfectant compositions with easily available raw materials.
  • Another object of this invention is to provide cleaner cum disinfectant compositions wherein less equipment is needed for their manufacture.
  • composition of dried Soap nut fruits, Neem leaf powder, salt, essential oil and water in the ratio of 100:50:10:10 and fresh tap water to make it to 1 Itr. composition.
  • composition comprising dried Soap nut fruits, Neem leaf powder, salt, essential oil and fresh tap water in the ratio of 100:50:10:10 and fresh tap water to make it to 1 Itr.
  • composition is prepared by a process comprising of the steps of procuring dried soap nut fruits, neem leaf powder along with salt and essential oil from reliable sources and mixing the same by carrying out different steps as mentioned in the detailed specification of the invention.
  • the present invention relates to disinfectant formulations comprising of all the compounds of natural origin.
  • the present invention relates to aqueous formulations suitable for disinfecting and cleaning large surfaces and comprising of all the compounds of natural origin.
  • the present invention relates to an aqueous disinfectant formulation
  • an aqueous disinfectant formulation comprising: all ingredients are of natural origin; a natural surfactant sufficient to form a solution or dispersion in an aqueous carrier (dried Soap nuts) a natural preservative (Salt) a natural antibacterial, antimicrobial, antiviral agent ( Neem leaf powder) a natural fragrance oil sufficient tap water to make it to 1000 ml. solution.
  • Microporous filter membrane can be used or any conventional ultra filtration membrane which has a mean actual pore size according to neem leaf powder mesh size can be used for filtration.
  • the aforesaid ingredients are mixed together in a homogenous aqueous mixture as described in the detailed description to obtain a cleaner cum disinfectant composition, which can be used to clean hard surfaces.
  • the concentrated composition can be used to remove hard stains like oil on wooden, marble, granite surfaces etc.
  • the natural ingredients used in the cleaner cum disinfectant composition of the present invention are as follows:
  • Soapnut Natural surfactants and emulsifiers
  • the chemical structure of saponins may be transformed during storage or processing.
  • the linkages between the sugarchain and the aglycones as well as between the sugar residues can undergo hydrolysis during acid or base treatment, hydrothermolysis or enzymatic/microbial transformations, resulting in the formation of a glycones, prosapogenins (partially hydrolyzed saponins), and sugar residues. Therefore, the selection of methods appropriate to the storage of plant material is a key part of each efficient technology.
  • Saponins due to the presence of a lipid-soluble a glycone and water-soluble sugar chain, show amphiphilic nature. In this way, foam formation (with liquid-gaseous phases), an emulgator effect (with liquid-liquid phases) and dispersion abilities (with liquid-solid phases) are achieved. Saponins with one sugar chain have the best foaming characteristics. The compounds with two or three sugar chains show decreasing of foaming ability. Some saponins without foaming character have also been observed.
  • aqueous solution saponin molecules align themselves vertically on the surface with their hydrophobic ends oriented away from the water. This has the effect of reducing the surface tension of the water, causing it to foam.
  • surfactants form micelles above a critical concentration called critical micelle concentration (CMC). Below this concentration, molecules remain unassociated. Micelles have a lipophilic centre, and this creation of a fat-loving compartment explains why detergents can dissolve grease and oil.
  • micelles are dependent on the type of saponin.
  • the micelle shapes depend on the saponinmolecule. For example, micelles formed by Saponaria and Quillaya saponins are elongated or even filamentous, while those formed by saponins of G. max are rather circular. Probably, the reason for these differences is the chemical structure of a glycone.
  • carboxylic acid in the saponin molecule may strongly influence the surface activity. Additionally, the location of this acid in the molecule is particularly important.
  • G. max saponin contains -COOH group in its hydrophilic part. The carboxylic group dissociates in aqua phase and forms free carboxyl anion, responsible for increasing the solubility of saponin in the water environment.
  • saponins of Sapindusmukorossi also contain the carboxylic groups but they attach to the hydrophobic a glycone. In consequence of this mechanism, the dissociation level of -COOH groups is very low.
  • Saponins can be used to enhance both the effectiveness of cleaning/disinfection processes. They are considered natural detergents and are used as additives in washing powders, and additives for liquid/powder cleaning. The addition of a small amount of saponin to an aqueous environment provides a product that is an effective water clarifier and solid surface cleanser. These compositions may be used to clean metals, metal-plated surfaces, ceramics, wood, glass, etc. The use of natural plant products as detergents could provide cheaper, safer and more consumer-acceptable alternatives to synthetic compounds. Soapnut possesses a soap-like attribute, it is used to prepare cleansing lotion, shampoo, protein shampoo as well as protein shampoo added with conditioner. This fruit encloses elevated amounts of saponins.
  • Soapnut is pulverized into a powder and used for cleansing hair, skin as well as washing laundry.
  • the soapnut fruit pulp encloses elevated amounts of natural foaming substances and often used in the form of a surfactant.
  • saponins are considered to be environment friendly and also safe for use.
  • Natural surfactants have great advantages as an eco-friendly alternative to synthetic surfactants. Studies showed that the surface-active properties of these nonionic surfactants are better than the synthetic surfactants like SLS. (Sodium Lauryl Sulphate)
  • Compostable - Used shells can be thrown in your compost.
  • Soap nut has natural anti-fungal properties
  • Leaf extracts Active constituents of neem leaf extract include isomeldenin, nimbin, nimbinene, 6-desacetyllnimbinene, nimbandiol, immobile, nimocinol, quercetin, and beta-sitosterol.
  • Neem leaf extracts have a powerful antiseptic, antifungal, antiviral and antibacterial effects. Unlike synthetic chemicals that often produce side effects such as allergic reactions, rashes etc., neem is gentle and does not create any complications. Unlike neem seed oil, neem leaves have a pleasant odour.
  • neem leaves are now known to contain Nimbin, nimbinenedesacetylnimbinase, nimbandial, nimbolide and quercetin. Neem leaves have shown potential in the following areas: Studies indicate that tender leaves are effective in Parasitic infections. A 10% aqueous extract of tender leaves has been found to possess anti-viral properties. The essential oil from fresh leaves has a mild fungicidal action.
  • Sea salt is salt that is produced by the evaporation of seawater. It is used as a seasoning in foods, cooking, cosmetics and for preserving food. It is also called bay salt, solar salt, or salt. Like mined rock salt, production of sea salt has been dated to prehistoric times.
  • sea salts on the market today vary widely in their chemical composition. Although the principal component is sodium chloride, the remaining portion can range from less than 0.2 to 10% of other salts. These are mostly calcium, potassium, and magnesium salts of chloride and sulfate with substantially lesser amounts of many trace elements found in natural seawater. Though the composition of commercially available salt may vary.
  • Table salt is an ionic compound, which breaks into its component ions or dissociates in water. These ions are Na+ and C1-. The sodium and chlorine atoms are present in equal amounts (1:1 ratio), arranged to form a cubic crystal lattice.
  • the molecular formula of table salt — sodium chloride — is NaCl.
  • each ion is surrounded by six ions having an opposite electrical charge.
  • the arrangement forms a regular octahedron.
  • the chloride ions are much larger than the sodium ions.
  • the chloride ions are arranged in a cubicarray with respect to one another, while the small sodium cations fill the gaps between the chloride anions.
  • Sodium chloride is vital for living organisms and important for industry. Most of the salinity of seawater is due to sodium chloride. Table salt is used to preserve food and enhance flavour.
  • the herbal ingredient i.e. soap nuts and neem leaf powder required for the cleaner cum disinfectant compositions are procured from APMC market and local shops and rest of the ingredients are procured from the local market and online sources.
  • the oil used for the preparation is acquired from reputed online suppliers.
  • the other raw materials such as salt are procured from a local shop.
  • the water used for soaking the dried soap nut fruits preparation is fresh, clean tap water.
  • the undiluted composition removes heavy oil stains easily from surfaces like wood, marble and tiles.
  • E.coli shows 5 log reduction - KS 929:2015
  • Staphylococcus aureus shows 5 log reduction - KS 929:2015
  • Salmonella shows 5 log reduction - KS 929:2015 Observed Shelf life: Recommended to use within 60 days of manufacturing subject to proper filtration, packing & storage.
  • Waste Being Biodegradable, waste material decomposes.
  • this Biodegradable waste material can be used as a raw material for the preparation of another natural product.
  • the mixture is then kept as it is for 48 hrs, which is again filtered through very fine cotton cloth (if required multiple filtrations done till getting clear liquid) and got the clear greenish-brown liquid to that added;
  • Staphylococcus aureus shows 5 log reduction - KS 929:2015
  • Salmonella shows 5 log reduction - KS 929:2015
  • Shelf life Recommended to use within 60 days of manufacturing subject to proper filtration, packing and storage.
  • Packing Being a natural composition, store in an amber coloured glass bottle, for better shelf life and efficacy, protect it from direct sunlight.
  • Waste Being Biodegradable, waste material decomposes.
  • this Biodegradable waste material can be used as a raw material for the preparation of another natural product.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Organic Chemistry (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Veterinary Medicine (AREA)
  • Medical Informatics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Inorganic Chemistry (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Detergent Compositions (AREA)

Abstract

La présente invention porte sur une formulation de nettoyage et désinfectante écologique comprenant un mélange de noix de lavage séchées, de poudre de feuille de neem séchée, de sel, d'huile essentielle et d'eau de robinet fraîche et sur son procédé de préparation. Les compositions de nettoyage et désinfectantes de la présente invention présentent une forme aqueuse et sont écologiques, faciles à préparer et économiques. La composition de nettoyage et désinfectante est biodégradable et possède des propriétés antibactériennes, antimicrobiennes et antifongiques.
PCT/IB2021/060121 2020-11-05 2021-11-02 Composition de nettoyage et désinfectante écologique WO2022097018A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN202021048374 2020-11-05
IN202021048374 2020-11-05

Publications (1)

Publication Number Publication Date
WO2022097018A1 true WO2022097018A1 (fr) 2022-05-12

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Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE TKDL 17 March 2010 (2010-03-17), "Ritha/bundaqulhindi, knowledge known since 1000 years", XP055939167, retrieved from http://www.tkdl.res.in Database accession no. AH1/40 *
DOWN TO EARTH: "Make floor cleaners and dish-wash at home using reetha or soapnuts with Kirti Negi Bajoria", YOUTUBE, 18 February 2018 (2018-02-18), XP055939093, Retrieved from the Internet <URL:https://www.youtube.com/watch?v=3otEjPI14UY> *
JEROBIN JAYAKUMAR, POOJA MAKWANA, RS SURESH KUMAR, RAJIV SUNDARAMOORTHY, AMITAVA MUKHERJEE, NATARAJAN CHANDRASEKARAN: "Antibacterial activity of neem nanoemulsion and its toxicity assessment on human lymphocytes in vitro", INTERNATIONAL JOURNAL OF NANOMEDICINE, vol. 10, no. 1 Suppl, 1 October 2015 (2015-10-01), pages 77 - 86, XP055939171 *
SUKUMARAN PREETHI: "The right mix", THE HINDU, 27 May 2016 (2016-05-27) *

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