WO2022075297A1

WO2022075297A1 - Food and drink, anti-fatigue agent, and endurance improver

Info

Publication number: WO2022075297A1
Application number: PCT/JP2021/036738
Authority: WO
Inventors: 浩彦中村; 史子齋藤; 梨絵小池
Original assignee: 森永乳業株式会社
Priority date: 2020-10-05
Filing date: 2021-10-05
Publication date: 2022-04-14
Also published as: JPWO2022075297A1

Abstract

Provided is a novel food and drink which can continuously increase the lactic acid concentration in the blood.　This food and drink contains a lactic acid compound and fructose, wherein the content of the lactic acid compound is 3-75 g in terms of lactic acid, and the content of the fructose is 7-150 g.

Description

Food and drink, anti-fatigue agents, and endurance improvers

The present invention relates to foods and drinks useful for anti-fatigue or endurance improvement, and also relates to anti-fatigue agents and endurance improving agents.

As foods and drinks to be ingested during or before and after exercise, various foods and drinks containing an active ingredient having a function of assisting activation of muscle activity and reduction of muscle fatigue are known. For example, Patent Document 1 discloses a food composition for recovering muscle fatigue associated with exercise, which contains hesperidins and a milk fat globules as active ingredients.

In recent years, attention has been paid to the action of lactic acid in blood. Non-Patent Document 1 discloses that lactic acid produced in fast muscle is carried to slow muscle and used for ATP production under oxidative conditions. Non-Patent Document 2 reports that high-intensity training increases blood lactate concentration and promotes mitochondrial adaptation, that is, improvement of metabolism. Non-Patent Document 3 reports that lactic acid in blood causes muscle hypertrophy.

The above-mentioned actions as described in Non-Patent Documents 1 to 3 brought about by an increase in lactic acid in blood are useful for activating muscle activity and reducing muscle fatigue. In addition, Non-Patent Document 4 discloses that when a large amount of fructose is ingested, the blood lactate concentration increases.

Japanese Unexamined Patent Publication No. 2019-182803

An object of the present invention is to provide a new food or drink, an anti-fatigue agent, and an endurance improving agent capable of continuously increasing the blood lactic acid concentration.

The present invention relates to [1] to [14] exemplified below.
[1] A food or drink containing a lactic acid compound and fructose, wherein the content of the lactic acid compound is 3 g or more and 75 g or less in terms of lactic acid, and the content of the fructose is 7 g or more and 150 g or less.

[2] The food or drink according to [1], wherein the lactic acid compound is at least one selected from the group consisting of lactic acid, potassium lactate, calcium lactate, iron lactate, and sodium lactate.

[3] The food or drink according to [1] or [2], which is a beverage or a powdered beverage prepared and used as a beverage.

[4] The food or drink according to [3], which is a packaged beverage having a total volume of 100 mL or more and 500 mL or less.

[5] Contains lactic acid compounds and fructose,
The content of the lactic acid compound is 0.6% by mass or more and 30% by mass or less in terms of lactic acid.
A food or drink composition having a fructose content of 1.4% by mass or more and 60% by mass or less.

[6] An agent for increasing blood lactic acid concentration, which contains a lactic acid compound and fructose as active ingredients.
[7] An agent which is an anti-fatigue agent or an endurance improving agent containing a lactic acid compound and fructose as active ingredients.

[8] The agent according to [7], which is a muscle activity activator or a muscle fatigue reducing agent.
[9] The agent according to [7], which is an anti-fatigue agent for physical fatigue.

[10] The agent according to [7], which is an anti-fatigue agent for mental fatigue.
[11] Any one of [7] to [10], wherein the lactic acid compound is used in a dose of 3 g or more and 75 g or less and fructose is used in a dose of 7 g or more and 150 g or less per adult. The agent described in the section.

[12] Use of lactic acid compounds and fructose in the production of anti-fatigue compositions or endurance-enhancing compositions.

[13] An anti-fatigue method comprising a step of administering a lactic acid compound and fructose to a mammal.
[14] A method for improving endurance, which comprises a step of administering a lactic acid compound and fructose to a mammal.

According to the present invention, it is possible to provide a new food or drink, an anti-fatigue agent, an endurance improving agent, a muscle activity improving agent, and a muscle fatigue recovering agent capable of continuously increasing the blood lactic acid concentration. ..

<Food and drink>
The food or drink according to the present invention (hereinafter, also simply referred to as “food or drink”) contains a lactic acid compound and fructose, the content of the lactic acid compound is 3 g or more and 75 g or less in terms of lactic acid, and the content of fructose is 7 g or more. It is 150 g or less. The food and drink preferably has a lactic acid compound content of 5 g or more and 50 g or less, and a fructose content of 20 g or more and 100 g or less. The content of the lactic acid compound in the food and drink is more preferably 15 g or more and 20 g or less in terms of lactic acid, and the fructose content is more preferably 30 g or more and 50 g or less. According to the food and drink according to the present invention, the blood lactic acid concentration of the ingested individual can be continuously increased.

The food and drink may be ingested per meal, may be ingested once, or may be ingested in multiple times. When ingested multiple times, it is preferable that at least 3 g of lactic acid compound and at least 7 g of fructose are ingested into the body at the same timing from the viewpoint of increasing the blood lactate concentration more continuously. .. As the same timing as used herein, for example, within 10 minutes is preferable, and within 5 minutes is more preferable. Food and drink may be provided in individual packages.

The food or drink composition according to the present invention (hereinafter, also simply referred to as “food and drink composition”) has a lactic acid compound content of 0.6% by mass or more and 30% by mass or less in terms of lactic acid, and a fructose content of 1. It is 4% by mass or more and 60% by mass or less. The fructose content is preferably 30% by mass or less, more preferably 15% by mass or less. According to the food and drink composition according to the present invention, the blood lactic acid concentration of the ingested individual can be continuously increased. The food and drink composition can be used, for example, to prepare the above-mentioned food and drink according to the present invention.

The lactic acid compound contained in the food or drink and the food or drink composition is preferably one or more selected from the group consisting of lactic acid, potassium lactate, calcium lactate, iron lactate, and sodium lactate. The lactic acid compound contained in the food or drink and the food or drink composition may be lactic acid, lactate, or one added and contained as a material containing these.

The fructose contained in the food and drink and the food and drink composition may be fructose or one added and contained as a material containing fructose. Examples of the material containing fructose include high fructose syrup, isomerized sugar such as fructose-glucose syrup, and fruit juice.

The food and drink and the food and drink composition according to the present invention can continuously increase the blood lactic acid concentration of the ingested individual. To continuously increase the blood lactate concentration of an ingested individual means to increase the blood lactate concentration of the ingested individual at a specific time after ingestion as compared with the case of not ingesting. It means that it has at least one effect of being able to, or being able to maintain a high value for a certain period of time after ingestion. Therefore, the food and drink and the food and drink composition according to the present invention can also be used as a lactic acid concentration increasing agent. According to the food and drink and the food and drink composition according to the present invention, the blood lactic acid concentration of the ingested individual achieves a value of, for example, 2 mmol / L or more at rest, or, for example, 2 mmol / L or more. The time to maintain the value can be lengthened, and in the case of exercise, for example, a value of 4 mmol / L or more can be achieved, or the time to maintain a value of, for example, 4 mmol / L or more can be lengthened. The effect of can be expected.

Lactic acid in blood is known to act effectively on activating muscle activity and reducing muscle fatigue. Therefore, the food and drink and the food and drink composition according to the present invention can be used both as a muscle activity activator that promotes activation of muscle activity in exercise such as sports and training, and as a muscle fatigue reducing agent that reduces muscle fatigue due to exercise. Can be used.

In the present specification, human "exercise" means muscles such as sports, walking, jogging, aerobic exercise, swimming, cycling, etc., which are intentionally and intentionally carried out for health, physical fitness, and enjoyment. It is an activity that uses. Further, in the present specification, "exercise" includes physical activities in daily life such as labor and housework. Activation of muscle activity can improve the amount of exercise, the duration of exercise, and the quality of exercise. That is, when muscle activity is activated, the time of exercise becomes longer instead of fatigue, the distance traveled by exercise increases, the speed during exercise increases, and the movement and sharpness of the body during exercise increase. do. When muscle fatigue is reduced, the degree of fatigue is reduced even if exercise is performed, and recovery is quick even if fatigue is caused by exercise.

Foods and drinks and food and drink compositions are preferably taken before, after, or during exercise because they have the effect of activating muscle activity and reducing muscle fatigue.

The food and drink and the food and drink composition according to the present invention have an anti-fatigue effect and an endurance-enhancing effect, and when a human takes them, they exhibit an anti-fatigue effect and / or an endurance-improving effect.

"Fatigue" refers to a phenomenon in which the function of exercise and mental activity declines as a result of exercise and mental activity, and is broadly classified into physical fatigue and mental fatigue. The anti-fatigue action according to the present invention may be an anti-fatigue action against physical fatigue or an anti-fatigue action against mental fatigue. As used herein, human "exercise" is as described above. As used herein, the term "mental activity" in humans refers to mental activity that accompanies human logical comprehension. Specific contents of human mental activities include thinking work, calculation work, computer work, operation of smartphones, and the like. Examples of human mental activities include work and study. The fatigue associated with the above-mentioned mental activity of a human is, for example, a state after one to two hours of continuous work on a personal computer, although there are individual differences.

The "anti-fatigue action" is an action of reducing such a "fatigue" state and an action of promoting recovery from the "fatigue" state by eating and drinking the food or drink or the food or drink composition according to the present invention. Alternatively, it refers to the action of preventing "fatigue" and making it less tiring.

The "anti-fatigue effect" on physical fatigue is evaluated by confirming, for example, that, as a result of the subject's exercise, the motor function does not decrease or the motor function improves, as in the examples described later. Can be done. The "anti-fatigue effect" on physical fatigue can, for example, activate muscle activity and reduce muscle fatigue as described above. It can also improve exercise performance.

The "anti-fatigue effect" on mental activity can be evaluated by, for example, performing a Kraepelin test or ATMT (Advanced Trail Making Test) as in the examples described later. The "anti-fatigue effect" on mental activity can, for example, reduce subjective fatigue. In addition, attention, concentration, and memory can be suppressed, maintained, and improved.

The Kraepelin test is a continuous addition work originally used for the purpose of mental examination, specifically, it is a work of adding side-by-side single-digit numbers in order, and is a work of advanced mental activity. It can be positioned as work that causes fatigue (mental fatigue, cerebral fatigue, etc.). Here, the Kraepelin test can be used for quantification as a model system for computational power, computer work, continuous thinking, and the like.

ATMT is originally used as a mental function test method, and is a visual search task in which the target numbers presented on the touch panel are pressed in order. The search reaction time for each target can be recorded, and the target is re-targeted for each reaction. It can be placed or newly generated. Therefore, it is also possible to measure the working memory and the degree of fatigue during task execution.

"Endurance" refers to the ability to continue to perform certain exercises and mental activities, and "endurance improving action" refers to the action to enhance the ability to continue. The "endurance improving effect" can be confirmed, for example, by extending a certain degree of luck or duration of mental activity, and by confirming that the quality of continuous exercise or mental activity is above a certain level. can.

The "endurance improving effect" can be evaluated by confirming that a constant exercise state can be continuously performed as a result of the subject's exercise, for example, as in the examples described later. The "endurance improving effect" on exercise allows, for example, to maintain and improve the performance of continuous exercise. The "endurance improving effect" on mental activity can suppress, maintain, and improve attention, concentration, and memory deterioration in continuous mental activity.

The food or drink and the food or drink composition may consist only of a lactic acid compound and fructose, and may contain other components that are permitted to be contained in the food or drink in addition to the lactic acid compound and fructose. The food or drink and the food or drink composition may be in the form of liquid, gel, paste, solid, powder or the like, and may also include tablets, liquid foods and the like. Foods and drinks and food and drink compositions include, for example, flour products, instant foods, processed agricultural products, processed marine products, processed livestock products, milk and dairy products, fats and oils, basic seasonings, complex seasonings and foods, and frozen foods. , Confectionery, gelled foods, beverages, commercial products other than these, and the like.

Examples of wheat flour products include bread, macaroni, spaghetti, noodles, cake mix, fried chicken flour, bread crumbs and the like. Examples of instant foods include instant noodles, cup noodles, retort / cooked foods, canned foods, microwave foods, instant soups / stews, instant miso soups / soups, canned soups, freeze / dry foods, and other instant foods. .. Examples of processed agricultural products include canned agricultural products, canned fruits, jams and marmalades, pickles, boiled beans, dried agricultural products, cereals (processed grain products) and the like. Examples of processed fishery products include canned fishery products, fish hams and sausages, fish paste products, fishery delicacies, and tsukudani. Examples of processed livestock products include canned livestock and pastes, livestock ham and sausage. Examples of milk and dairy products include fermented milk, processed milk, milk drinks, yogurts, lactic acid bacteria drinks, cheese, ice creams, prepared milk powders, creams, and other dairy products. Examples of fats and oils include butter, margarines, vegetable oils and the like. Examples of the basic seasoning include soy sauce, miso, sauces, processed tomato seasonings, mirin, vinegar and the like. Examples of complex seasonings / foods include cooking mixes, curry ingredients, sauces, dressings, noodle soups, spices, and other complex seasonings. Examples of the frozen food include raw material frozen food, semi-cooked frozen food, and cooked frozen food. Examples of confectionery include caramel, candy, chewing gum, chocolate, cookies, biscuits, cakes, pies, snacks, crackers, Japanese confectionery, rice confectionery, bean confectionery, dessert confectionery, and other confectionery. Examples of the gel-like food include foods that utilize the thermal coagulation property of proteins (pudding, chawanmushi, etc.), and jelly-like foods in which a mixed solution containing an aqueous raw material is made into a jelly by a gelling agent. Examples of the jelly-like food include jelly-like foods such as fruit flavor, coffee flavor, milk flavor, green tea flavor, and almond jelly flavor, jelly-like sports beverage, jelly-like oral rehydration solution, and nutritional supplement jelly. Beverages include, for example, carbonated beverages, natural fruit juices, fruit juice beverages, refreshing beverages containing fruit juice, fruit meat beverages, fruit beverages containing fruit grains, vegetable beverages, soy milk, soy milk beverages, coffee beverages, tea beverages, powdered beverages, concentrated beverages, sports. Beverages, oral rehydration solutions, nutritional beverages, alcoholic beverages, other favorite beverages and the like can be mentioned. Examples of commercially available foods other than the above include baby foods, sprinkles, and ochazuke paste.

The food and drink and the food and drink composition can be produced by a method known to those skilled in the art, and may be produced by adding a lactic acid compound and fructose to the known food and drink, or in the raw material of the food and drink. It can also be mixed to produce new foods and drinks and food and drink compositions. In the production of foods and drinks and food and drink compositions, in addition to the steps of adding and mixing lactic acid compounds and fructose, molding steps, sterilization steps, fermentation steps, baking steps, drying steps, cooling steps, granulation steps, packaging steps, etc. Can be combined as appropriate.

Preferred forms of food and drink include sports drinks taken during exercise, before or after exercise, drinks such as oral rehydration solutions, and powdered drinks prepared and used as drinks.

In addition to lactic acid compounds and fructose, various components usually contained in beverages, such as sweeteners other than fructose, acidulants, vitamins such as vitamin A, vitamin B, and vitamin E, and antioxidants are included in beverages. , Preservatives such as sodium benzoate, thickeners, emulsifiers, sodium, calcium, magnesium, potassium, zinc, iron, manganese, copper and other minerals, sodium glutamate, lysine hydrochloride, carnitine citrulin, arginine, ornithine, etc. Amino acids and their salts, herbal fragrances, apple, grape, grapefruit, pineapple, peach, lemon, plum, orange and other fruit juices and their concentrated juices, royal jelly, galana extract, caffeine, garlic extract, maca extract, raw medicine, A seaweed extract or the like can be arbitrarily added as long as the effect of the present invention is not impaired.

Examples of sweeteners other than fructose include sugars such as sucrose, glucose, granulated sugar, lactose, and maltose, and low-sweetness sweeteners such as xylitol and D-sorbitol. Examples of the acidulant include citrates such as trisodium citrate, anhydrous citric acid, adipic acid, gluconic acid, succinic acid, tartrate acid, fumaric acid, malic acid, phytic acid, ascorbic acid and salts thereof.

The pH of the beverage at a temperature of 20 ° C. is preferably 3.5 to 4.6, more preferably 3.7 to 4.2, and even more preferably 3.8 to 4.1. This makes it possible to maintain a good aftertaste. The pH can be adjusted, for example, by adjusting the type and amount of the lactic acid compound, or by using a pH adjusting agent.

Beverages can be obtained, for example, by uniformly mixing lactic acid compounds, fructose, and other components added as needed with water according to a conventional method. The beverage may be a packaged beverage that has been sterilized by heating and packed in a container. Examples of the packaged beverage include a packaged beverage having a total volume of 100 mL or more and 500 mL or less. The timing of blending each component is not limited to before the sterilization treatment, and a specific component, for example, an aroma component having a relatively high volatility may be blended after the sterilization treatment.

Examples of the container include a sealed container made of a single substance such as glass, paper, plastic (polyethylene terephthalate, etc.), aluminum, and steel, or a composite material or laminated material thereof. The type of container is not particularly limited, and examples thereof include PET bottles, aluminum cans, steel cans, paper packs, chilled cups, and bottles. From the viewpoint of handleability, distribution, portability, etc., the container is preferably a paper pack or a PET bottle.

Foods and drinks and food and drink compositions can be provided and sold as foods and drinks and food and drink compositions labeled with health uses such as activation of muscle activity, reduction of muscle fatigue, anti-fatigue, and improvement of endurance. Is. Such indications are not particularly limited, but for example, "improve exercise performance", "reduce the feeling of fatigue caused by exercise", "prevent muscle cramps during and after exercise, and leg cramps (so-called" fishing "). "Reduce and improve", "Reduce the feeling of fatigue caused by work and study", "Maintain / improve memory", "Maintain / improve attention", "Maintain / improve concentration", etc. Be done. The act of "displaying" includes all actions for informing the consumer of the above-mentioned use, and if the expression is such that the above-mentioned use can be recalled or inferred, the purpose of the display, the content of the display, and the display are used. Regardless of the object, medium, etc., all fall under the "display" act of the present invention.

"Indication" also includes labeling as health foods, functional foods, enteral nutrition foods, special purpose foods, health functional foods, specified health foods, nutritional functional foods, functionally labeled foods, non-medicinal products, etc. Can be mentioned. Among these, in particular, labeling approved by the Consumer Affairs Agency, for example, a system related to foods for specified health use, nutritionally functional foods, or foods with functional claims, or labeling approved by a system similar to these can be mentioned. Specifically, labeling as a food for specified health use, labeling as a food for specified health use with conditions, labeling as a food with functional claims, labeling to the effect that it affects the structure and function of the body, labeling for reducing disease risk, science The display of functionality based on the scientific basis can be mentioned.

<Agent>
The agent according to the present invention is a blood lactate concentration increasing agent, an anti-fatigue agent, an endurance improving agent, a muscle activity activator, or a muscle fatigue reducing agent.

The blood lactic acid concentration increasing agent (hereinafter, also simply referred to as "blood lactic acid concentration increasing agent") according to the present invention contains a lactic acid compound and fructose as active ingredients. Here, including as an active ingredient means that it is contained in an effective amount that can increase the lactic acid concentration in blood. As for the lactic acid compound and fructose, the above description of the lactic acid compound and fructose contained in the food and drink is applied.

Increasing the blood lactate concentration means that the blood lactate concentration of the administered individual can be increased at a specific time after administration, or the value can be increased at a certain period after administration. It means having at least one action of being able to maintain. The blood lactate concentration increasing agent may further contain other components known to increase the blood lactate concentration.

Lactic acid in blood is known to act effectively on activating muscle activity and reducing muscle fatigue. Therefore, the blood lactate concentration increasing agent can be used both as a muscle activity activator that promotes activation of muscle activity in exercise such as sports and training, and as a muscle fatigue reducing agent that reduces muscle fatigue due to exercise.

The anti-fatigue agent according to the present invention (hereinafter, also simply referred to as "anti-fatigue agent") contains a lactic acid compound and fructose as active ingredients. Here, including as an active ingredient means that it is contained in an effective amount capable of exerting an anti-fatigue effect. As for the lactic acid compound and fructose, the above description of the lactic acid compound and fructose contained in the food and drink is applied. As for the anti-fatigue effect, the above description of the anti-fatigue effect in foods and drinks is applied. The anti-fatigue agent according to the present invention may be the blood lactate concentration increasing agent itself according to the present invention, or may further contain a blood lactate concentration increasing agent and other components, and may be used as other components. Includes other ingredients known to have anti-fatigue activity.

The endurance improving agent according to the present invention (hereinafter, also simply referred to as "endurance improving agent") contains a lactic acid compound and fructose as active ingredients. Here, including as an active ingredient means that it is contained in an effective amount capable of exerting an endurance improving effect. As for the lactic acid compound and fructose, the above description of the lactic acid compound and fructose contained in the food and drink is applied. As for the endurance improving action, the above-mentioned explanation of the endurance improving action in foods and drinks is applied. The endurance improving agent according to the present invention may be the blood lactate concentration increasing agent itself according to the present invention, may further contain a blood lactate concentration increasing agent and other components, and may be another component. Examples include other components known to have an endurance-enhancing effect.

The muscle activity activator according to the present invention (hereinafter, also simply referred to as “muscle activity activator”) is the blood lactate concentration increasing agent, the anti-fatigue agent, or the endurance improving agent itself according to the present invention. It may further contain a blood lactate concentration increasing agent and other components, and other components include other components known to activate muscle activity.

The muscle fatigue reducing agent according to the present invention (hereinafter, also simply referred to as “muscle fatigue reducing agent”) may be the blood lactate concentration increasing agent or the anti-fatigue agent itself according to the present invention, and may be the blood lactate concentration. It may further contain an elevating agent and other components, and other components include other components known to suppress muscle fatigue.

Examples of the route of administration of the blood lactic acid concentration increasing agent, anti-fatigue agent, endurance improving agent, muscle activity activator, and muscle fatigue reducing agent include parenteral administration such as oral administration and enteral administration. Examples of the dosage form of the blood lactic acid concentration increasing agent include sprays, capsules, tablets, granules, syrups, emulsions, suppositories, injections, ointments, tapes and the like. The doses of lactic acid compounds and fructose, which are the active ingredients of blood lactate concentration-increasing agents, anti-fatigue agents, endurance enhancers, muscle activity activators, and muscle fatigue-reducing agents, are determined by the dosage form, symptoms to be administered, and age. In order to effectively increase the blood lactic acid concentration, the dose of lactic acid compound should be 3 g or more and 75 g or less and lactic acid should be 7 g or more and 150 g or less per adult. It is preferable to have. Mammals are exemplified as the administration target. Examples of mammals include humans, cows, sheep, goats, pigs, dogs, cats, horses and the like.

For formulation, additives such as excipients, binders, disintegrants, lubricants, stabilizers, flavoring agents, diluents, and solvents for injections can be used. In the case of formulation, the content of each of the lactic acid compound and fructose in the formulation is preferably 1 to 50% by mass, particularly preferably 3 to 30% by mass, although it depends on the dosage form.

Examples of excipients include cellulose derivatives such as crystalline cellulose, hydroxypropyl cellulose, hydroxypropylmethyl cellulose, carboxymethyl cellulose, and carboxymethyl cellulose calcium; gum arabic; dextran; purulan; light anhydrous silicic acid, synthetic aluminum silicate, magnesium aluminometasilicate. And the like; silicate derivatives such as calcium phosphate; carbonate derivatives such as calcium carbonate; sulfate derivatives such as calcium sulfate.

Examples of the binder include gelatin; polyvinylpyrrolidone; tunagor and the like in addition to the above-mentioned excipients.

Examples of the disintegrant include, in addition to the above-mentioned excipients, chemically modified starch or cellulose derivatives such as croscarmellose sodium, carboxymethyl starch sodium, and crosslinked polyvinylpyrrolidone.

Examples of the lubricant include talc; stearic acid; stearic acid metal salts such as calcium stearate and magnesium stearate; colloidal silica; waxes such as bee gum and gay wax; boric acid; glycol; carboxylic acids such as fumaric acid and adipic acid. Carboxylic acid sodium salt such as sodium benzoate; Sulfate salt such as sodium sulfate; Leucine; Lauryl sulfate such as sodium lauryl sulfate and magnesium lauryl sulfate; Silic acid such as anhydrous silicic acid and silicic acid hydrate; starch derivative etc. Can be mentioned.

Examples of the stabilizer include paraoxybenzoic acid esters such as methylparaben and propylparaben; alcohols such as chlorobutanol, benzyl alcohol and phenylethyl alcohol; benzalkonium chloride; acetic anhydride; sorbic acid and the like.

Examples of the flavoring agent include sweeteners, acidulants, and fragrances.
Examples of the solvent for injection include water, ethanol, glycerin and the like.

<Use of lactic acid compounds and fructose in the production of compositions and compositions>
The composition according to the present invention is a composition for increasing blood lactate concentration, a composition for anti-fatigue, a composition for improving endurance, a composition for activating muscle activity, or a composition for reducing muscle fatigue. Lactic acid compounds and fructose are used in the production of the composition according to the present invention. As for the lactic acid compound and fructose, the above description of the lactic acid compound and fructose contained in the food and drink is applied.

The composition for increasing the blood lactate concentration according to the present invention contains the above-mentioned blood lactate concentration increasing agent, and may be the above-mentioned blood lactate concentration increasing agent itself. The anti-fatigue composition according to the present invention contains the above-mentioned anti-fatigue agent, and may be the above-mentioned anti-fatigue agent itself. The composition for improving endurance according to the present invention contains the above-mentioned endurance improving agent, and may be the above-mentioned endurance improving agent itself. The composition for activating muscle activity according to the present invention contains the above-mentioned muscle activity activator, and may be the above-mentioned muscle activity activator itself. The composition for reducing muscle fatigue according to the present invention contains the above-mentioned muscle fatigue reducing agent, and may be the above-mentioned muscle fatigue reducing agent itself.

Examples of the form of the composition according to the present invention include a pharmaceutical composition, a food and drink composition, and a feed composition. In the case of a pharmaceutical composition, it is preferable to prepare the pharmaceutical composition by adding any additive such as a pharmaceutically acceptable excipient together with a lactic acid compound and fructose to formulate the desired dosage form. In the case of a food or drink composition, the above description of the form of the food or drink composition is applied.

Examples of the feed composition include pet food, livestock feed, fish feed and the like. The feed composition is prepared by mixing a lactic acid compound and fructose with a general feed or its raw materials such as grains, lees, brans, fish meal, bone meal, fats and oils, skim milk powder, whey, mineral feed, and yeasts. It can be manufactured by doing so. The produced feed composition can be orally administered to general mammals, livestock, fish farms, pet animals and the like.

<Method of increasing blood lactic acid concentration, anti-fatigue method, endurance improvement method, muscle activity activation method, muscle fatigue reduction method>
The method for increasing the blood lactic acid concentration, the anti-fatigue method, the endurance improving method, the method for activating muscle activity, and the method for reducing muscle fatigue according to the present invention are to administer a lactic acid compound and fructose to a mammal. Including the process of When the lactic acid compound and fructose are administered and the lactic acid compound and fructose are present in the body at the same timing, the concentration of lactic acid in the blood can be increased, the anti-fatigue effect can be exhibited, and the endurance is enhanced. Can be lactic acid, can activate muscle activity, or can reduce muscle fatigue. Examples of mammals include humans, cows, sheep, goats, pigs, dogs, cats, horses and the like.

Hereinafter, the present invention will be described in more detail with reference to examples, but the present invention is not limited thereto.

<Test Example A>
[Beverage]
Lactic acid compounds and fructose were added to water to prepare beverages 1 to 7 having a total amount of 200 ml containing the amounts of lactic acid (total amount of lactic acid compounds in terms of lactic acid) and fructose shown in Table 1. As raw materials for lactic acid compounds, lactic acid powder (manufactured by Corbion Japan Co., Ltd.), calcium lactate powder (manufactured by Corbion Japan Co., Ltd.), sodium lactate solution (manufactured by Corbion Japan Co., Ltd.), potassium lactate solution (co. Bion Japan Co., Ltd.) was used, and these were appropriately used so that the total amount in terms of lactic acid was the value shown in Table 1 and the pH of the beverage was 3.7 or more and 4.0 or less. The total amount in terms of lactic acid may be simply referred to as the content of "lactic acid" below. Pure fructose (manufactured by Kato Chemical Co., Ltd.) was used as a raw material for fructose.

[Test Examples 1 to 7]
In Test Examples 1 to 7, the subjects were allowed to ingest the beverages 1 to 7, respectively, and the effect of oral ingestion of the beverage according to the present invention on the blood lactate concentration was confirmed. Three healthy men and women (ages 30 to 60 years) were used as subjects, and the same three subjects were used in Test Examples 1 to 7. Test Examples 1 to 7 were performed on different days.

(Test Example 1)
In a measuring room with a temperature of 24 ° C. and a relative humidity of 50%, the subject received the measurement while sitting. After fasting for 4 hours, the subject collected blood from the fingertips and measured the blood lactate concentration with a measuring device manufactured by ARKRAY (product name: Lactate Pro 2 LT-1730). After that, the subject ingested the beverage 1. Then, the blood lactate concentration was measured every 10 minutes after ingestion of the beverage 1 until 60 minutes had passed by the same method as described above. The subject was fasted until 60 minutes after ingesting the beverage 1. Based on the measurement results, the time (in 10-minute increments) at which the blood lactate concentration was maintained at 2 mol / L or higher was calculated for each subject, and the average time of the three subjects was calculated. The results are shown in Table 2.

(Test Examples 2 to 7)
In Test Examples 2 to 7, the same tests as in Test Example 1 were carried out using beverages 2 to 7, respectively, instead of the beverage 1 used in Test Example 1. Then, the average time of the three subjects was calculated in the same manner as in Test Example 1. The results are shown in Table 2.

From the results shown in Table 2, when the beverages 2 to 5 containing 7 g or more of fructose and 3 g or more of lactic acid were ingested, the beverage 1 containing no lactic acid was ingested, and the beverage 6 containing no lactic acid was ingested. And, as compared with the case of ingesting the beverage 7 having a fructose content of 5.0 g, the time when the lactic acid concentration in the blood is 2 mmol / L or more is longer, and there is an excellent effect in increasing the lactic acid concentration in the blood. I understand.

[Test Examples 8 to 10]
In Test Examples 8 to 10, the subjects were ingested the beverages 1, 3 and 6, respectively, and then exercise was applied, and the effect of oral administration of the beverage according to the present invention on the blood lactic acid concentration was confirmed. The subjects were three healthy men and women (ages 30 to 60 years). In Test Examples 8 to 10, the same three subjects were used. Test Examples 8 to 10 were performed on different days.

(Test Example 8)
The measurement was performed in a measurement room having a temperature of 24 ° C. and a relative humidity of 50%, and the subject received the measurement while sitting. After fasting for 2 hours or more, the subject collected blood from the fingertips and measured the blood lactate concentration with a measuring device manufactured by ARKRAY (product name: Lactate Pro 2 LT-1730). The subject was then ingested Beverage 1 and subjected to low intensity bicycle ergometer exercise for 60 minutes. Then, the blood lactate concentration was measured every 10 minutes after ingestion of the beverage 1 until 60 minutes had passed by the same method as described above. Based on the measurement results, the time (in 10-minute increments) at which the blood lactate concentration was maintained at 4 mmol / L or higher was calculated for each subject, and the average time of the three subjects was calculated. The results are shown in Table 3.

(Test Examples 9 and 10)
In Test Examples 9 and 10, the same test as in Test Example 8 was performed using beverages 3 and 6, respectively, instead of the beverage 1 used in Test Example 8. Then, the average time of the three subjects was calculated in the same manner as in Test Example 8. The results are shown in Table 3.

From the results shown in Table 3, when the beverage 3 containing fructose and lactic acid was ingested, the lactic acid in the blood during exercise was compared with the case where the beverage 1 without lactic acid and the beverage 6 without fructose were ingested. It was found that the concentration was 4 mmol / L or more for a long time, and that it had an excellent effect on increasing the blood lactate concentration during exercise.

<Test Example B>
In Test Example B, the subjects were allowed to ingest the three types of beverages B1, B2, and B3 shown in Table 4, respectively, to obtain an anti-fatigue effect against fatigue caused by oral ingestion of the beverage according to the present invention and an effect of improving endurance in exercise. evaluated. Powdered water candy (M-SPD-M manufactured by Showa Sangyo Co., Ltd.) was used to prepare beverage B2, and lactic acid powder (fermented by Corbion Japan Co., Ltd.) was used as a raw material for lactic acid compounds to prepare beverage B3. Lactate powder 60), calcium lactate powder (fermented calcium lactate manufactured by Corbion Japan Co., Ltd.), sodium lactate solution (60% fermented sodium lactate manufactured by Corbion Japan Co., Ltd.), potassium lactate solution (Corbion Japan Co., Ltd.) ), Puresal (registered trademark) HiPure P Plus) was used, and pure lactate (manufactured by Kato Chemical Co., Ltd.) was used as a raw material for lactate.

Four healthy men and women (ages 25 to 60 years old) were used as subjects, and each subject took a crossover method in which they ingested the three types of beverages shown in Table 4 in a random order. The test was carried out in a measuring room having a temperature of 24 ° C. and a relative humidity of 50%. After fasting for 2 hours or more, the subject measured the maximum exerted power (hereinafter, also referred to as "P1") with a bicycle ergometer (PowerMax VII manufactured by Combi, anoxic power test), and 10 minutes later, PWC (maximum load). (Amount) 75% intensity, constant intensity bicycle exercise at 60 rpm was loaded up to all-out or up to 60 minutes. Immediately after the exercise was completed, the test drink was given as a rest, and 60 minutes later, the maximum exerted power (hereinafter, also referred to as “P2”) was measured again. Table 4 shows the average value of the ratio of P2 to P1 of the three different test beverages.

In Table 4, ^* p <0.1 is represented, and the p value is the result of comparison with the beverage B3. From the results shown in Table 4, the intake of beverage B3 has a smaller decrease in maximum exerted power after 60 minutes of exercise and a reduction in muscle fatigue compared to the intake of water (beverage B1) and a beverage containing dextrin (beverage B2). It is clear that the decrease in exercise performance is suppressed, and it is found that the effect of anti-fatigue against fatigue caused by exercise and the effect of improving endurance in exercise are exhibited.

<Test Example C>
In Test Example C, the subjects were allowed to ingest the three types of beverages C1, C2, and C3 shown in Table 5, respectively, and the anti-fatigue effect against fatigue due to mental activity due to oral ingestion of the beverage according to the present invention was evaluated. Lactic acid powder, calcium lactate, sodium lactate, and potassium lactate (all manufactured by Corbion Japan Co., Ltd.) are used as raw materials for lactic acid compounds, and pure fructose (Kato Kagaku (Kato Kagaku)) is used as a raw material for fructose to prepare beverages C2 and C3. Co., Ltd.) was used.

Seven healthy men and women (ages 25 to 60 years old) were the subjects, and each subject ingested three types of beverages in a random order in a crossover manner. The test was carried out in a measuring room having a temperature of 24 ° C. and a relative humidity of 50%. After fasting for 2 hours or more, the subjects were asked to perform the Uchida-Kraepelin test for 15 minutes and ATMT performed on a personal computer for 15 minutes as cognitive tasks, and measured the correct number and number of tasks, respectively (measured values before ingestion). .. Five minutes later, the test beverage was ingested, and then the Uchida-Kraepelin test and ATMT were performed again for 15 minutes each, and the correct number and number of each task were measured (measured values after ingestion). Table 5 shows the average value of the change in the measured value after ingestion from the measured value before ingestion of 7 subjects.

In addition, questions (VAS (Visual Analog Scale)) were asked before the measurement of the cognitive task before ingestion of the test beverage and after the measurement of the cognitive task after ingestion of the test beverage, and self-evaluation was performed. In the VAS, the current state is written by a vertical line on a line segment of 100 mm, and 0 mm is not related to the state at all, and 100 mm is the maximum that can be experienced. Table 5 shows the average value of changes in self-evaluation before and after the cognitive task for the three items of fatigue, attention, and concentration.

In Table 5, ^* p <0.1 is represented, and the p value is the result of comparison with the beverage C3. In the results shown in Table 5, the beverage C3 has a small increase in the degree of fatigue due to mental activity with respect to the intake of water (beverage C1) and the beverage containing lactic acid but not fructose (beverage C2). The decrease in concentration was suppressed, and the increase in task performance was large in both the Uchida Kleperin test and ATMT. From the results shown in Table 5, it was found that the beverage C3 exerts an anti-fatigue effect and an endurance improving effect.

Claims

Contains lactic acid compounds and fructose,
The content of the lactic acid compound is 3 g or more and 75 g or less in terms of lactic acid.
A food or drink having a fructose content of 7 g or more and 150 g or less.
The food or drink according to claim 1, wherein the lactic acid compound is at least one selected from the group consisting of lactic acid, potassium lactate, calcium lactate, iron lactate, and sodium lactate.
The food or drink according to claim 1 or 2, which is a beverage or a powdered beverage prepared and used as a beverage.
The food or drink according to claim 3, which is a packaged beverage having a total volume of 100 mL or more and 500 mL or less.
Contains lactic acid compounds and fructose,
The content of the lactic acid compound is 0.6% by mass or more and 30% by mass or less in terms of lactic acid.
A food or drink composition having a fructose content of 1.4% by mass or more and 60% by mass or less.
A blood lactic acid concentration increasing agent containing a lactic acid compound and fructose as active ingredients.
An agent that is an anti-fatigue agent or an endurance improving agent that contains a lactic acid compound and fructose as active ingredients.
The agent according to claim 7, which is a muscle activity activator or a muscle fatigue reducing agent.
The agent according to claim 7, which is an anti-fatigue agent for physical fatigue.
The agent according to claim 7, which is an anti-fatigue agent for mental fatigue.
The agent according to any one of claims 7 to 10, wherein the lactic acid compound is used in a dose of 3 g or more and 75 g or less and fructose is 7 g or more and 150 g or less per adult. ..
Use of lactic acid compounds and fructose in the production of anti-fatigue compositions or endurance-enhancing compositions.
An anti-fatigue method comprising the step of administering a lactic acid compound and fructose to a mammal.
A method for improving endurance, which comprises a step of administering a lactic acid compound and fructose to a mammal.