WO2022035719A1 - Système et méthodes de traitement de cellules cancéreuses avec des champs magnétiques à polarité alternée - Google Patents

Système et méthodes de traitement de cellules cancéreuses avec des champs magnétiques à polarité alternée Download PDF

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Publication number
WO2022035719A1
WO2022035719A1 PCT/US2021/045132 US2021045132W WO2022035719A1 WO 2022035719 A1 WO2022035719 A1 WO 2022035719A1 US 2021045132 W US2021045132 W US 2021045132W WO 2022035719 A1 WO2022035719 A1 WO 2022035719A1
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Prior art keywords
magnetic field
cancer cells
cells
frequency
body area
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PCT/US2021/045132
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English (en)
Inventor
Vivek K. SHARMA
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Sharma Vivek K
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Priority claimed from US17/308,019 external-priority patent/US11344739B2/en
Priority claimed from US17/396,608 external-priority patent/US11344740B2/en
Application filed by Sharma Vivek K filed Critical Sharma Vivek K
Priority to IL303111A priority Critical patent/IL303111A/en
Priority to EP21759494.4A priority patent/EP4208252A1/fr
Publication of WO2022035719A1 publication Critical patent/WO2022035719A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N2/00—Magnetotherapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N2/00—Magnetotherapy
    • A61N2/004—Magnetotherapy specially adapted for a specific therapy

Definitions

  • the present invention involves treating rapidly proliferating or dividing cells, such as cancer cells, and more specifically to systems and methods for selectively inhibiting or destroying rapidly dividing cells by applying an alternating magnetic field having defined characteristics to a target area of a patient’s body.
  • Some embodiments of the invention provide a wearable system capable of providing an ambulatory therapy to a non- stationary patient by applying a magnetic field to inhibit or destroy rapidly dividing cells to the target body area.
  • Cell division is a reproductive process in all living systems, including without limitation simple one-celled organisms such as bacteria and protozoa, as well as more complex organisms such as algae, plants, and animals, including humans.
  • the cell division cycle involves a series of events within the cell that leads to a duplication of the DNA of the cell, with one of the duplicate DNA sequences going to each of two daughter cells.
  • Prokaryotic cells are one-celled organisms that lack an enclosed nucleus and reproduce by a cell division process known as fission. More complex organisms with enclosed nuclei are called eukaryotes, whose cells asexually reproduce by a three-part cell division process involving periods known as interphase, mitosis, and cytokinesis. In the reproduction of sexual cells (i.e. , egg and sperm) of more complex organisms, mitosis is replaced by meiosis.
  • Mitosis involves separation of the duplicated DNA in the nucleus of the eukaryotic cell into two nuclei, each having a complete copy of the duplicated DNA.
  • cytokinesis the cytoplasm, organelles & cell membrane are divided, forming two daughter cells of roughly equal size.
  • the process of mitosis is further divided into the stages of prophase, prometaphase, metaphase, anaphase, and telophase.
  • prophase the DNA duplicated during interphase condenses into discrete long, thin chromosomes having two chromatids joined by a centromere.
  • Each cell has two centrioles, which move to opposite poles of the cell during prophase.
  • Microtubules radiate from near the two centrioles toward the center of the cell, including some which extend to the chromatids and help to separate the two chromatids into separate daughter chromatids.
  • the chromosomes move toward the cell equator and align in the metaphase plane (or equatorial plane).
  • the daughter chromatids separate from each other at the equator during early anaphase by moving along the microtubule spindle fibers toward the centromeres at opposite poles of the cell, a process which elongates the cell.
  • the daughter chromosomes In late anaphase the daughter chromosomes each reach their opposite poles of the cell, and the cell membrane begins to pinch to form the two daughter cells, which is part of cytokinesis, or the process by which the daughter cells are separated.
  • the microtubules continue to lengthen and a new nuclear envelope forms around each of the separated daughter chromosomes, each of which has an identical set of chromosomes, and cytokinesis proceeds with further pinching of the two daughter cells toward becoming separate entities.
  • the microtubule spindles disappear.
  • the daughter cells fully separate, completing cytokinesis.
  • Cancer cells and some non-cancerous cells proliferate or grow in an uncontrolled manner in contrast to normal cells. In addition to the extra space such tumors or cells occupy, they may also damage nearby normal cells. Cancer cells may also metastasize, traveling to other locations in the body, where they continue to hyperproliferate and may form new tumors. The rapid growth of tumors and cancer cells results from their rapid rate of cell division compared to normal cells.
  • One type of electrical therapy involves applying an electrical current through body tissue separated by two or more conductive electrodes.
  • This type of therapy may be used, for example to stimulate or excite muscle or nerve tissue (e.g., pacemakers, defibrillators, neurostimulators) or to generate heat within a desired body tissue (e.g., thermal therapies to remodel collagen or to ablate tissue).
  • Electrical therapies involving conductive electrodes may involve direct current or alternating current at a wide range of frequencies (e.g., less than 1 Hz to above 1 MHz). The energy from electrical currents is delivered to tissue based on the electrical conductive characteristics (e.g., resistance, capacitance) of the tissue.
  • conductive electrodes may be used to stimulate muscle or nerve tissue to activate muscle or nerve fibers.
  • stimulation with conductive electrodes is too rapid for stimulation signals to propagate through such tissue and the signals are “shorted.”
  • Another medical use of electrical energy involves the use of insulated electrodes to deliver high frequency electrical energy radiatively or inductively to target tissue.
  • radio frequency (RF) or microwave energy may be applied radiatively to tissue through the air or another electrically insulating material separating the electrodes from the tissue being treated.
  • RF radio frequency
  • the effect of this type of electrical energy on living tissue is based on the dielectric properties of the tissue rather than their conductive characteristics.
  • insulated electrodes have been used to treat cancer cells and other rapidly proliferating cells by applying AC electric fields at frequencies of 50-500 kHz and electric field strengths of about 10 - 1000 V/m to a target body area that includes such cells.
  • Such therapy is often referred to as TC (“tumor curing”) field or TTF (“tumor treatment field”) therapy.
  • TTF therapy also involves the use of relatively high voltages. For this reason, patients must be careful in performing everyday activities having a risk of water exposure (e.g., showering, exercise (sweating), or even exposure to rain).
  • Electrodes in direct contact with the patient’s skin presents a risk of burning or heating of tissue adjacent to the electrodes. Because of this risk (and buildup of dirt, oils, etc.), the electrodes in TTF therapy systems typically require frequent replacement (e.g., twice each week). Patients wearing TTF electrodes on the scalp reported headaches related to wearing the electrodes 24 hours a day.
  • TTF electrodes must also be placed by trained users (e.g., technicians or physicians). Because the treatment is highly localized (i.e., between the electrodes), precise location of the cancer/tumor must first be performed, and the electrodes must be placed with a high degree of accuracy to create an electric field that passes through it. If the electrodes are slightly off of optimal placement, the treatment may result in suboptimal results.
  • the ‘289 patent discloses ambulatory embodiments (i.e., embodiments in which the patent can wear and use the system in performing at least some ordinary non-stationary life activities such as walking, driving, shopping, etc.), in practice the power requirements (e.g., high voltages) for generating appropriate electric fields (e.g., at least 10 V/m) result in bulky and/or heavy electronics boxes that must be coupled to the electrodes and thus carried by the patient.
  • appropriate electric fields e.g., at least 10 V/m
  • One clinical study showed a relatively high rate of falls in patients carrying these cumbersome TTF electronics boxes.
  • TTF systems there is a need for safer therapies that may be applied for longer durations to destroy cancer or other rapidly-dividing cells.
  • the present invention provides a system for treating cancer cells in a target body area of a patient comprising: an alternating polarity (AP) magnetic field generator; one or more AP electromagnetic coils coupled to the AP magnetic field generator and to a target body area, wherein the one or more electromagnetic coils are energized by an electrical signal from the AP magnetic field generator to generate an AP magnetic field having at least a first frequency and a first field strength; and a controller for controlling one or more of the AP magnetic field generator and the one or more AP electromagnetic coils to generate and apply to the target body area an AP magnetic field having a frequency of 0.1 - 500 kHz and a field strength of 0.05-5 mT, wherein the AP magnetic field selectively affects the cancer cells to achieve at least one of damaging the cancer cells, inhibiting the growth of the cancer cells, reducing tumor size, inhibiting angiogenesis, eliciting an immune response to the cancer cells, increasing tumor immunogenicity, decreasing immunosuppressive activity of the cancer cells, recruiting
  • AP magnetic field selective
  • the present invention provides a system for treating cancer cells in a target area of a patient’s body comprising: at least one electromagnetic coil coupled to a target body area; and a controller for controlling the at least one electromagnetic coil to generate and apply to the target body area an alternating polarity (AP) magnetic field having a frequency of 0.1 - 500 kHz and a field strength of 0.05 - 5 mT, wherein the AP magnetic field selectively affects the cancer cells to achieve at least one of damaging the cancer cells, inhibiting the growth of the cancer cells, reducing tumor size, inhibiting angiogenesis, eliciting an immune response to the cancer cells, increasing tumor immunogenicity, decreasing immunosuppressive activity of the cancer cells, recruiting one of antigen-presenting cells and immune effector cells to the tumor microenvironment (TME), or preventing metastasis of the cancer cells, while leaving non-cancer cells substantially unharmed.
  • AP alternating polarity
  • the present invention provides a system for treating cancer cells in a target body area of a patient comprising: an alternating polarity (AP) magnetic field generator; one or more AP electromagnetic coils coupled to the AP magnetic field generator and to a target body area, wherein the one or more electromagnetic coils are energized by an electrical signal from the AP magnetic field generator to generate an AP magnetic field having at least a first frequency and a first field strength; a controller for controlling the AP magnetic field generator and the one or more AP electromagnetic coils to generate and apply to the target body area an AP magnetic field having a frequency of 0.1 - 500 kHz and a field strength of 0.1 -5 mT, wherein the AP magnetic field modifies the cancer microenvironment (TME) by at least one of: increasing the number of CD8+ lymphocytes in the TME; increasing the ratio of CD8+ to total lymphocytes in the TME; increasing the number of CD4+ lymphocytes; increasing the ratio of CD4+ to total lymphocytes
  • TME cancer microenviron
  • the present invention provides a system for treating cancer cells in a target body area of a patient comprising: an alternating polarity (AP) magnetic field generator; at least one electromagnetic coil coupled to the AP magnetic field generator and to a target body area, wherein the at least one electromagnetic coil is energized by an electrical signal from the AP magnetic field generator to generate an AP magnetic field having at least a first frequency and a first field strength; and a controller for controlling the at least one electromagnetic coil and power supply to generate and apply to the target body area an AP magnetic field having a frequency of 0.1 - 500 kHz and a field strength of 0.1 -5 mT, wherein the AP magnetic field modifies the cancer microenvironment (TME) by at least one of: modulating the blood vessels surrounding the cancer cells; modulating the presence of fibroblasts proximate to the cancer cells; modulating immune cell signaling molecules proximate to the cancer cells; modulating the extracellular matrix surrounding the cancer cells; modulating resident host cells; modulating infil
  • TME cancer microenviron
  • the present invention provides a method of treating cancer cells in a target body area of a patient, comprising: providing a magnetic field therapy system comprising: an alternating polarity (AP) magnetic field generator; one or more AP electromagnetic coils coupled to the AP magnetic field generator, wherein the one or more AP electromagnetic coils are energized by an electrical signal from the AP magnetic field generator to generate an AP magnetic field having at least a first frequency and a first field strength; and a controller to control at least one of the first frequency and the first field strength of the AP magnetic field generated by the one or more AP electromagnetic coils; coupling the one or more AP electromagnetic coils to the target body area; generating an AP magnetic field having a first frequency of 0.1 -500 kHz and a field strength of 0.2-5 mT using the one or more AP electromagnetic coils; applying the generated AP magnetic field to the target body area using the one or more AP electromagnetic coils, wherein the AP magnetic field modifies the tumor microenvironment
  • the present invention comprises a method of treating cancer cells in a target body area of a patient, comprising: providing a magnetic field therapy system comprising: an alternating polarity (AP) magnetic field generator; one or more AP electromagnetic coils coupled to the AP magnetic field generator, wherein the one or more AP electromagnetic coils are energized by an electrical signal from the AP magnetic field generator to generate an AP magnetic field having at least a first frequency and a first field strength; and a controller to control at least one of the first frequency and the first field strength of the AP magnetic field generated by the one or more AP electromagnetic coils; coupling the one or more AP electromagnetic coils to the target body area; generating an AP magnetic field having a first frequency of 0.1 -500 kHz and a field strength of 0.2-5 mT using the one or more AP electromagnetic coils; applying the generated AP magnetic field to the target body area using the one or more AP electromagnetic coils, wherein the AP magnetic field modifies the tumor microenvironment to
  • Figure 1 is a schematic block diagram of a system for providing an alternating polarity (AP) magnetic field to a target body area of a patient’s body, according to one embodiment for selectively destroying cancer cells.
  • AP alternating polarity
  • Figure 2 is a front view of a retaining element comprising a bra having one or more AP electromagnetic coils for providing an AP magnetic field to breast tissue, according to one embodiment of the invention.
  • Figure 3 is a front view of a retaining element comprising a hat including one or more AP electromagnetic coils for providing an AP magnetic field to brain tissue, according to one embodiment of the invention.
  • Figure 4 is a front view of a retaining element comprising a shirt having one or more AP electromagnetic coils for providing an AP magnetic field to thoracic or abdominal tissue, according to one embodiment of the invention.
  • Figure 5 is a front view of a retaining element comprising a neck cuff or collar having one or more AP electromagnetic coils for providing an AP magnetic field to a target area of a patient’s body, according to one embodiment of the invention.
  • Figure 6 is a front view of a retaining element comprising a bandage having one or more AP electromagnetic coils for providing an AP magnetic field to a target area of a patient’s body, according to one embodiment of the invention.
  • Figure 7A is a photograph at 10X magnification of untreated B16F10 mouse melanoma cells incubated for 24 hours.
  • Figure 7B is a photograph of B16F10 mouse melanoma cells exposed to an AP magnetic field for 24 hours according to one embodiment of the invention.
  • Figure 8 is a bar graph showing the reduction in cell counts of B16F10 mouse melanoma cells treated with an AP magnetic field for 24 hours compared to untreated controls.
  • Figure 9 is a graph of tumor volume as a function of time from the start of a magnetic field tumor therapy in an experiment according to one embodiment of the invention.
  • Figure 10 is a schematic block diagram of one embodiment of a system for providing an alternating polarity (AP) magnetic field to a target body area of a patient’s body for treating cancer cells.
  • AP alternating polarity
  • Figure 11 is a table summarizing the results of a number of experiments on cancer cells using alternating polarity (AP) magnetic field tumor (MFT) therapy.
  • AP alternating polarity
  • MFT magnetic field tumor
  • Figure 12 is a graph showing the increase in tumor volume over time for breast cancer cells in an animal model experiment involving MFT therapy.
  • Figure 13 is a graph showing the increase in tumor volume over time for colon cancer cells in animals treated with an immunotherapy alone or combined with MFT therapy.
  • Figure 14 is a graph showing relative metastasis of breast cancer cells in animals treated with MFT therapy, an immunotherapy, or combined MFT therapy and immunotherapy.
  • Figure 15 is a graph showing relative changes of CD8+ T cells in the tumor microenvironment of breast cancer cells in animals treated with MFT therapy, an immunotherapy, or combined MFT therapy and immunotherapy.
  • Figure 16 is a graph showing relative changes of myeloid-derived suppressor cells (MDSCs) in the tumor microenvironment of breast cancer cells in animals treated with MFT therapy, an immunotherapy, or combined MFT therapy and immunotherapy.
  • MDSCs myeloid-derived suppressor cells
  • Figure 17 is a graph showing relative changes of dendritic cells (DCs) in the tumor microenvironment of breast cancer cells in animals treated with MFT therapy, an immunotherapy, or combined MFT therapy and immunotherapy.
  • DCs dendritic cells
  • the invention provides apparatus and methods for treating a patient having cancer or other rapidly dividing cells (e.g., bacterial infection) in a target body area using alternating polarity (AP) magnetic fields at specified frequencies to destroy or inhibit the proliferation of the rapidly dividing cells.
  • AP alternating polarity
  • the use of electric fields, including without limitation TTF systems, to treat patients having cancer or other diseases characterized by rapidly-dividing cells has a number of limitations that make treatment for some patients difficult, ineffective, painful, or unsafe.
  • Embodiments of the present invention overcome one or more of these limitations by using AP magnetic fields to treat rapidly-dividing or hyperproliferating cells.
  • MFT therapy and “MFTT” refer to systems and methods for treating cancer or other rapidly-dividing cells with AP magnetic fields at specified frequencies and magnetic field strengths to destroy or inhibit the proliferation of such cells.
  • the present invention may be used to treat one or more cancers such as throat cancer, thyroid cancer, mouth cancer, nose cancer, salivary gland cancer, lung cancer, lung carcinoid tumors, thymic malignancies, tracheal tumors, pancreatic cancer, liver cancer, stomach cancer, kidney cancer, ovarian cancer, prostate cancer, colon cancer and rectal cancer.
  • duty cycle refers to the fraction of a time period in which an alternating polarity magnetic field is applied to the target body area.
  • the MFT therapy is applied in repeating cycles of on time followed by off time.
  • an 80% duty cycle may involve 8 hrs in which an alternating polarity magnetic field is applied to the target body area, followed by 2 hrs in which no magnetic field is applied. It will be appreciated that the same duty cycle can be achieved over different time frames, for example 8 minutes on time followed by 2 minutes off time, or 8 days on time followed by 2 days off time.
  • FIG. 1 is a simplified schematic block diagram illustrating certain components of an MFT therapy system 100 according to an embodiment of the invention.
  • the MFT therapy system 100 includes an alternating polarity magnetic field generator (APMFG) 110 to generate an electrical signal to energize one or more alternating polarity (AP) electromagnetic coils 120 to produce an AP magnetic field having specified frequency and field strength characteristics.
  • the AP electromagnetic coils 120 may have sizes and shapes adapted to engage one or more target body areas of a patient (e.g., torso, breast, head, neck, throat) for treatment of cancer or hyperproliferating cells in the target body area.
  • the electrical signals generated by APMFG 110 and applied to AP electromagnetic coils 120 is controlled by a controller 130, which specifies the parameters of the magnetic fields to be generated by APMFG 110 and AP electromagnetic coils 120, and controls the function and operation of the system 100.
  • An interface 140 is provided to allow a user to specify treatment parameters to be programmed or communicated to the controller 130, and to receive information from the controller relating to the operation and status of the MFT therapy system 100.
  • a power supply 150 provides power to MFT therapy system 100.
  • Power supply 150 may be selected from a variety of known power supplies, and may comprise, in various embodiments, a battery such as a disposable or rechargeable battery, or a power source such as a standard 120V, 60 Hz electrical power outlet in the US, together with circuitry for regulating the power at appropriate currents and voltages for each of the APMFG 110, AP electromagnetic coils 120, controller 130, and interface 140.
  • a battery such as a disposable or rechargeable battery
  • a power source such as a standard 120V, 60 Hz electrical power outlet in the US, together with circuitry for regulating the power at appropriate currents and voltages for each of the APMFG 110, AP electromagnetic coils 120, controller 130, and interface 140.
  • Controller 130 may include circuitry and other components (e.g., microcontrollers, resistors, registers, memory, firmware, software, etc.) to direct and control the operations of the APMFG 110, AP electromagnetic coils 120, and interface 140.
  • Figure 1 illustrates an embodiment in which the AP electromagnetic coils 120 are energized directly from the magnetic field generator.
  • controller 130 may communicate directly with each of the one or more AP electromagnetic coils 120 to control their operation in whole or in part (e.g., by switches that enable or disable each AP electromagnetic coil 120).
  • Controller 130 includes a timing control module 112 for controlling the timing of the MFT therapy delivered by AP electromagnetic coil(s) 120 to one or more target body areas or tissues.
  • timing control module 112 may cause the AP magnetic field generator 110 and AP electromagnetic coils 120 to provide MFT therapy for a programmed duration such as 1 -100 hours or other treatment period, or the timing of and between a plurality of therapy treatment periods.
  • the timing control module 112 may implement a first therapy for a first time period (e.g., during waking hours of the patient) at a first frequency and field strength, followed by a second time period in which no therapy is applied, followed by a third time period in which a second therapy is implemented at a second frequency and second field strength.
  • Timing control module 112 may also control the timing of changes in other treatment parameters, such as changes in the frequency or field strength of the MFT therapy applied to the patient.
  • a frequency control module 114 controls the frequency of the AP magnetic fields delivered by AP electromagnetic coil(s) 120 to the one or more target body areas or tissues.
  • Frequency control module 114 may control the frequency of the AP magnetic field at a programmed frequency of 0.5 - 500 kHz.
  • the frequency control module 114 may control frequency changes to the AP magnetic fields generated by the APMFG 110 and the AP electromagnetic coils 120 at a programmed rate of change or according to specific frequency step changes.
  • a magnetic field strength control module 116 controls the field strength of the AP magnetic fields applied to the one or more target body areas.
  • Magnetic field strength control module 116 may control the field strength at a programmed magnetic field strength of 0.05 - 5 mT, and may control changes in the field strength according to a programmed rate of change or programmed step changes in field strength.
  • Controller 130 may include programming logic, timers, and other circuitry to accomplish the functions of the timing control module 112, frequency control module 114, and magnetic field strength control module 116. It will be appreciated in alternative embodiments, the functions of all or portions of timing control module 112, frequency control module 114, and magnetic field strength control module 116 may be combined into one or more submodules, or implemented by controller 130 as a whole.
  • interface 140 may include a user input, such as a keyboard or buttons, to allow a user to input or receive data from controller 130.
  • interface 140 may be located within controller 130 and may comprise a transceiver to communicate with a separate user device (not shown) such as a cell phone, tablet, or other computing device to program the MFT therapy system 100 and receive data therefrom (e.g., operating and alarm status flags, programmed parameters, treatment time, etc.).
  • a separate user device such as a cell phone, tablet, or other computing device to program the MFT therapy system 100 and receive data therefrom (e.g., operating and alarm status flags, programmed parameters, treatment time, etc.).
  • interface 140 may be omitted, or may be incorporated as part of a single unit having some or all of the functions of AP magnetic field generator 110, controller 130, and interface 140.
  • the APMFG 110 may provide an electrical signal to cause each of the one or more AP electromagnetic coils 120 to generate magnetic fields having one or more fixed or variable AP frequencies. Although shown in the simplified schematic diagram of Figure 1 as coupled to APMFG 110 by a single wire, it will be understood that each of AP electromagnetic coils 120 will generally be coupled to APMFG 110 by a pair of wires (not shown) to provide a complete circuit.
  • APMFG 110 may cause each of the one or more AP electromagnetic coils 120 to generate a magnetic field having a single frequency or a plurality of frequencies either continuously or intermittently according to a defined duty cycle (e.g., having a programmable on-time during which the magnetic field is emitted from AP electromagnetic coils 120, followed by an off-time during which no field is emitted).
  • the APMFG 110 may also cause the one or more AP electromagnetic coils 120 to generate AP magnetic fields having a variety of waveforms, e.g., sinusoidal, triangular, trapezoidal etc.
  • the APMFG 110 may cause the one or more AP electromagnetic coils 120 to generate AP magnetic fields having a pre-defined number of waveforms of a specified first frequency, and repeat this pattern at a second specified frequency (burst mode).
  • the APMFG 100 may cause the one or more AP electromagnetic coils 120 to generate a magnetic field having a waveform which uses a fixed frequency or a combination of frequencies coupled with amplitude modulation.
  • the frequency (or frequencies) of the AP magnetic fields generated by each AP electromagnetic coil 120 are preferably frequencies below about 1 MHz, and more preferably are frequencies within a range selected from 0.1 -500 kHz, 0.2-400 kHz, 0.5-300 kHz, 1 -200 kHz, 5-150 kHz, 10-100 kHz, or 25-100 kHz.
  • the MFT therapy system 100 may comprise at least two AP electromagnetic coils 120, each having a fixed or variable frequency within a different frequency range to provide magnetic fields at multiple frequencies to a target body area or tissue.
  • APMFT 110 may generate a first electrical signal to cause a first AP electromagnetic coil 120 to generate an AP magnetic field with a first fixed frequency or a variable first frequency within a first frequency range, and a second electrical signal to cause a second AP electromagnetic coil 120 to generate an AP magnetic field with a second fixed frequency or a variable second frequency within a second frequency range, where both the first frequency range and the second frequency range are ranges within the range of 0.1 - 500 kHz, 0.2-400 kHz, 0.5-300 kHz, 1 -200 kHz, 5-150 kHz, 10-100 kHz, or 25-100 kHz.
  • the first frequency range may be a low- frequency range (e.g., 1 - 5 kHz) and the second frequency range may be a higher- frequency range (e.g., 50 kHz - 300 kHz).
  • AP magnetic fields within a plurality of frequency sub-ranges within the range of 0.5 - 500 kHz may affect different aspects of the reproduction cycle of rapidly-dividing cells, and that each such aspect may be more strongly affected by AP magnetic fields within a particular frequency sub-range within the broader range of 0.5 - 500 kHz.
  • the interruption of angiogenesis by extremely low frequency AP magnetic fields has been reported for AP magnetic fields having a frequency of 50 Hz (Monache et al., “Inhibition of Angiogenesis Mediate by Extremely Low-Frequency Magnetic Fields (ELF-MFs),” PLOS One, 8:1 1 (Nov 2013).
  • ELF-MFs Extremely Low-Frequency Magnetic Fields
  • Different types of cells including without limitation different types of cancer cells, may require different frequencies for interruption of angiogenesis.
  • an MFT therapy having a bimodal magnetic field frequency distribution may be applied to the target body area.
  • the APMFT 110 may generate a first electrical signal to cause a first AP electromagnetic coil 120 to generate a first variable AP magnetic field distribution that varies the magnetic field frequency over a first time period (e.g., 1 second, 1 minute, 10 minutes, 1 hr) between a first lower limit (e.g., 0.5 kHz) and a first upper limit (e.g., 5 kHz) to broadly interrupt a first metabolic process (e.g., angiogenesis) in a target cell population, as defined by frequency control module 114.
  • a first time period e.g., 1 second, 1 minute, 10 minutes, 1 hr
  • a first lower limit e.g., 0.5 kHz
  • a first upper limit e.g., 5 kHz
  • the APMFT 110 may also generate a second electrical signal to cause the same or a second AP electromagnetic coil 120 to generate a second variable AP magnetic field distribution that varies the magnetic field frequency over a second time period (e.g., 1 second, 1 minute, 10 minutes, 1 hr) between a second lower limit (e.g., 50 kHz) and a second upper limit (e.g., 400 kHz) to broadly interrupt a second metabolic process (e.g., the mitosis cycle) of rapidly-dividing cells.
  • Additional coils may produce different fixed or variable-frequency AP magnetic fields having different frequencies or frequency ranges to interrupt still other aspects of the reproduction cycle of rapidly-dividing cells.
  • a single AP electromagnetic coil 120 may be used to sequentially deliver AP magnetic fields within two different AP frequency ranges (e.g., 1 - 5 kHz for a first treatment period, followed by 50 - 400 kHz for a second treatment period).
  • variable-frequency embodiments many different ways of implementing a changing frequency are possible, and enumeration herein of specific embodiments of varying frequencies is illustrative and is not intended to be limiting. It will be appreciated that additional variable-frequency embodiments may be implemented in view of the present disclosure.
  • a magnetic field may be generated having a single frequency that varies from a lower frequency (e.g., 25 kHz) to an upper frequency (e.g., 150 kHz) in a uniform manner (i.e., non-varying rate of frequency change) within a defined frequency range time period or at a desired (e.g., programmed) frequency change rate.
  • the frequency may vary in a non-uniform manner such as stepwise changes in frequency or different rates of change (e.g., rates of change of frequency are highest near the mid-point between the upper and lower frequency limits).
  • the frequency may vary continuously or intermittently, with variable-frequency periods alternating with non-variable frequency periods.
  • a field having two different frequencies may simultaneously be applied to the target body area (emitted, e.g., by a single coil or by two different coils).
  • MFT therapies having a desired frequency distribution (e.g., random, Gaussian, or non-Gaussian) either sequentially or simultaneously may be applied to one or more target areas.
  • MFT therapy systems 100 of the present invention may use relatively low magnetic field strengths to destroy or impair rapidly-proliferating cells.
  • MFT therapy fields in systems 100 of the present invention have field strengths of less than 5 milliTesla (i.e. , 5,000 pT), such as field strengths within a range selected from 0.1 -5 mT, 0.2-4 mT, 0.4-3 mT, 0.5-2 mT, or 0.8-1 .6 mT.
  • the field strengths are within the range of 0.2 - 4 mT, and more preferably within the range of 0.5 - 2 mT.
  • Table 1 summarizes frequency and field strength ranges according to various embodiments of the invention. In various embodiments, additional frequency ranges can be provided using upper or lower boundaries from different ranges provided in the table.
  • the magnetic field may have a single, non-varying field strength.
  • different cell sizes e.g., different types of cancers
  • the AP magnetic fields may have a single, non-varying field strength either continuously or intermittently according to a defined duty cycle as defined by, e.g., timing control module 112 and magnetic field strength control module 116.
  • variable-field-strength embodiments many different ways of varying the field strength can be envisioned, similar to the variations described above respecting frequency changes. As with frequency, enumeration herein of specific embodiments of varying field strength is illustrative, not limiting. Additional variable-field-strength embodiments may be implemented (e.g., by magnetic field strength control module 116) in view of this disclosure.
  • a magnetic field may have a field strength that varies from a lower limit (e.g., 0.05 mT) to an upper limit (e.g., 1 mT) in a uniform manner (i.e. , with a non-varying rate of change) within a defined field strength range time period.
  • the field strength may vary in a non-uniform manner such as stepwise changes in field strength or with a swept field strength variation with accelerating or decelerating field strength variation (e.g., rates of change of field strength are highest near the upper and lower limits of the field strength range).
  • magnetic field strength may vary continuously or intermittently, with variable-field-strength periods alternating with non-variable-field-strength periods.
  • AP magnetic fields at two different frequencies, each having a different field strength may simultaneously be applied to the target body area (emitted, e.g., by two different AP electromagnetic coils 120).
  • MFT therapies having a desired frequency and magnetic field strength distribution (e.g., random, Gaussian, or non-Gaussian), either sequentially or simultaneously may be applied to the target body area.
  • the therapy is suspended for certain periods. This may involve, for example, providing MFT therapy continuously with defined alternating on-time (e.g., a time period withing a range of 1 sec - 24 hr) and off-time (e.g., 1 sec - 24 hr) periods according to a defined treatment duty cycle as defined by timing control module 112.
  • on-time and off-time periods may be a time period within a range of 1 second - 1 week, 1 sec - 24 hr, 1 minute - 12 hr, etc.).
  • the MFT therapy is provided continuously at a 10:1 duty cycle by generating and applying the MFT therapy fields for ten (10) minutes, followed by 1 minute in which no therapy is applied, with the process repeated until a predefined total treatment duration (e.g., 2 weeks) is complete.
  • a predefined total treatment duration e.g. 2 weeks
  • the same 10:1 duty cycle may be administered by applying the MFT therapy fields for ten hours, followed by a one-hour suspension of therapy, and repeating the process until the total treatment period is complete.
  • MFT therapy according to a defined treatment duty cycle comprising on-time and off-time periods may be administered for a defined treatment duration (e.g., 1 minute, 1 hr, 6 hr, 8 hr, 24 hr) after which no further treatment is applied.
  • the MFT therapy may be administered according to the patient’s circadian rhythms (e.g., continuously at night or when the patient is sleeping, and according to a defined duty cycle for defined periods during the day such as morning hours, afternoon hours, or evening hours). It will be appreciated that other duty cycles and treatment durations may be used, and that the therapy may involve, as previously discussed, constant or variable magnetic field frequencies and field strengths.
  • MFT therapy may be applied according to a defined treatment duty cycle of on-time and off-time periods, with the magnetic field strength varying according to a defined field strength duty cycle. This may involve, for example, a 10:1 treatment duty cycle combined with a 4:1 field strength duty cycle. As a specific example, the MFT therapy may be provided for a 24 hr treatment duration, at a 10:1 treatment duty cycle with AP magnetic fields applied to a target body area for 10 minutes, followed by 1 minute in which no AP magnetic fields are applied.
  • 8 minutes may involve variable frequency treatment within a first field strength range of 3.0 - 4.0 mT, followed by 2 minutes of treatment within a second field strength range of 0.5 - 1 .5 mT, providing a 4:1 field strength duty cycle.
  • the AP magnetic fields in a mode known as “pulse mode” or “burst mode” in which pulses at a defined low frequency (the “pulse frequency”) and pulse duration are applied to a target body area, but each pulse comprises a higher frequency magnetic field signal at a frequency within a frequency range and field strength range previously described.
  • pulse mode or “burst mode” in which pulses at a defined low frequency (the “pulse frequency”) and pulse duration are applied to a target body area, but each pulse comprises a higher frequency magnetic field signal at a frequency within a frequency range and field strength range previously described.
  • AP magnetic field pulses at a pulse frequency of 50 Hz with each pulse having a pulse duration of 10 msec, are applied in which each 10 msec pulse comprises pulses having a higher frequency of 100 kHz.
  • Table 2 summarizes the exemplary pulse frequency and pulse durations for burst mode operation. Frequencies and field strengths within each pulse of Table 2 would be as shown in Table 1 .
  • MFT therapy fields may be applied according to the patient’s circadian rhythms, or according to specific times of day.
  • the MFT therapy fields may be applied to the target body area only during daytime hours; only during nighttime hours; during all daytime hours except during mealtime hours; during all daytime hours except when the patient is exercising (as detected by, e.g., an activity monitor); during specific hours of the day (e.g., 9:00AM-noon and 6:00PM-5:00AM).
  • specific hours of the day e.g., 9:00AM-noon and 6:00PM-5:00AM.
  • Most magnetic field-generating coils are constructed so as to generate a magnetic field having an axis along which the magnetic field lines are directed.
  • multiple AP magnetic coils 120 can be spatially aligned in such a manner that a desired magnetic field distribution is generated in area of interest, such as the entirety or a portion of the target body area.
  • one or more parameters defining the MFT therapy may be determined based on the results of certain tests.
  • an imaging procedure may be performed to identify the type and location of the target rapidly-dividing cells (e.g., cancer or tumor cells).
  • the imaging procedure may be an imaging procedure using one or more of an MRI system, a CT scan system, a PET scan system, and an X-ray system.
  • a healthcare provider such as a physician may select one or more parameters of the MFT therapy, including without limitation, the frequency/frequencies of the magnetic field(s), the field strength(s), the positioning of one or more coils, a coil size, a type of retaining element (e.g., a garment type) to maintain the coils in position relative to the target body area, a duty cycle or schedule for applying therapy, etc.
  • parameters of the MFT therapy including without limitation, the frequency/frequencies of the magnetic field(s), the field strength(s), the positioning of one or more coils, a coil size, a type of retaining element (e.g., a garment type) to maintain the coils in position relative to the target body area, a duty cycle or schedule for applying therapy, etc.
  • the foregoing and other parameters may also be selected based on other tests, e.g., a pathological analysis of the cancer cells such as a microscopic analysis of a biopsy, a chemical test, a genetic test, etc.
  • the results of an imaging procedure prior to the MFT therapy may identify a target body area to which MFTT is to be directed. Based on the location of the target body area, in some embodiments a retaining element may be necessary to retain the magnetic coils in a desired position relative to the target treatment area or tissue.
  • Various retaining elements may be used to unobtrusively and securely maintain the magnetic coils in a desired position relative to a desired target area of the patient’s body.
  • a bra may be used to house the magnetic coils for treatment of breast cancer cells.
  • a hat may be used to retain magnetic coils in position to treat brain cancer.
  • a neck cuff, collar, or scarf may retain one or more magnetic coils for treatment of esophageal cancer
  • a shirt may be used to retain one or more magnetic coils to treat lung cancer
  • the retaining element may be a bandage such as an adhesive bandage capable of adhering to the target body area or to skin adjacent thereto.
  • the retaining elements may include pouches or pockets for securely retaining the coils in position with a comfortable and biocompatible lining placed between the coil and the skin or target treatment area.
  • the AP electromagnetic coils 120 are completely integrated within the retaining element during manufacturing (e.g., the coils are completely integrated inside a garment such as a bra, hat, shirt, bandage, etc.)
  • the retaining element may include a lead wire for coupling each of the one or more coils 120 to the AP magnetic field generator 110 and/or controller 130.
  • a direct electrical coupling e.g., a snap fit
  • the electronics package may include one or more of the power supply 150, controller 130, APMFG 110, and interface 140.
  • FIG. 2 illustrates a bra 200 that acts as a retaining element for one or more magnetic coils 220 for applying one or more magnetic fields to a target body area to treat cancer cells or other rapidly-dividing cells in breast tissue.
  • Magnetic coils 220 may be the same as coils 120 described in Figure 1 , but may be adapted for placement in bra 200 (e.g., with a size, geometry, etc., for treatment of breast tissue).
  • Bra 200 may in many aspects be constructed similarly to existing bras available at retail clothing outlets, and may include cups 210 for holding breast tissue and retaining coils 220 in position relative to a target body area comprising breast tissue.
  • Straps 230 may be provided to secure the bra 200 to the shoulders of the patient, and side straps or bands 240 for securing the bra to the patient’s torso.
  • AP electromagnetic coils 220 may be integrated into bra 200, or may be removably coupled thereto.
  • One or more cables or wires 250 may be provided to couple each of the coils 220 to an electronics box 260, which may house the remaining components of the MFT therapy system 100 of Figure 1 such as APMFG 110, controller 130, power supply 150, and in some embodiments interface 140.
  • APMFG 110, controller 130, power supply 150, or interface 140 may be provided separately from the electronics box 260.
  • interface 140 may comprise a mobile phone app that communicates directly with one or more of APMFG 110, controller 130, power supply 150, etc., as well as receiving and displaying information from one or more of the foregoing system components.
  • the mobile phone app interface may allow the patient or a healthcare provider to program one or more treatment parameters for the MFT therapy system 100, and may display information relating to the MFT therapy or system 100 status (e.g., displaying how long the MFT therapy has been applied, whether a magnetic field is currently being applied to the target tissue from each of the coils 220, the frequency and/or field strength of the currently-provided magnetic fields, remaining battery life, etc.).
  • information relating to the MFT therapy or system 100 status e.g., displaying how long the MFT therapy has been applied, whether a magnetic field is currently being applied to the target tissue from each of the coils 220, the frequency and/or field strength of the currently-provided magnetic fields, remaining battery life, etc.
  • Figure 3 illustrates a hat 300 that acts as a retaining element for one or more AP electromagnetic coils 320 for applying one or more magnetic fields to the treatment of cancer or other rapidly-dividing cells in a target body area comprising brain tissue.
  • Magnetic coils 320 are, in one embodiment, similar to AP electromagnetic coils 120 described in Figure 1 , but may be adapted for placement in hat 300. This may include changes in the size, geometry, or other characteristics to enable effective placement in hat 300 for treatment of brain tissue.
  • methods and systems of the present invention may be used to treat a variety of brain cancers, including without limitation astrocytomas, glioblastoma multiforme, meningioma, and pituitary tumors.
  • hat 300 may be a skullcap of an appropriate size to fit closely on the head of the patient, and the magnetic coils 320 may be have a concave shape adapted for location or placement in the cap, e.g., inside the hat or in a pocket between an inner and outer layer thereof.
  • AP electromagnetic coils 320 may be integrated into hat 300, or may be removably coupled thereto.
  • One or more cables or wires 350 may be provided to couple each of the AP coils 320 to an electronics box 360, which may house the remaining components of the MFT therapy system 100 of Figure 1 such as APMFG 110, controller 130, power supply 150, and in some embodiments interface 140.
  • APMFG 110, controller 130, power supply 150, or interface 140 may be provided separately from the electronics box 360.
  • a separate interface 140 may be provided as a mobile phone app that communicates directly with one or more of APMFG 110, controller 130, power supply 150, etc.
  • Such an app-based interface may also provide information on the MFT therapy to the patient or a healthcare provider (e.g., displaying how long the MFT therapy has been applied, whether a magnetic field is currently being applied to the target tissue from each of the coils 320, the frequency and/or field strength of the currently-provided magnetic fields, remaining battery life, etc.).
  • a healthcare provider e.g., displaying how long the MFT therapy has been applied, whether a magnetic field is currently being applied to the target tissue from each of the coils 320, the frequency and/or field strength of the currently-provided magnetic fields, remaining battery life, etc.
  • Figure 4 illustrates a shirt 400 that acts as a retaining element for one or more AP electromagnetic coils 420 for applying one or more magnetic fields to the treatment of cancer or other rapidly-dividing cells in a target tissue in a patient’s thoracic or abdominal region.
  • This may include, without limitation and depending on the placement of the one or more AP electromagnetic coils 420, treatment of lung cancer, liver cancer, pancreatic cancer, or cancers or tumors in other thoracic or abdominal organs or structures.
  • AP electromagnetic coils 420 are, in one embodiment, similar to coils 120 described in Figure 1 , but may be adapted for placement in shirt 400 based on the target tissue.
  • shirt 400 may include changes in the coil size, geometry, or other characteristics to enable effective placement in shirt 400 and for treatment of the particular target tissue.
  • shirt 400 may comprise other garments that may cover the thoracic or abdominal region of a patient, including sweaters, jackets, coats, etc., although in preferred embodiments a shirt that fits tightly to the patient’s body is used to better retain the AP electromagnetic coils 420 in a more precise or controlled placement relative to the target tissue.
  • AP electromagnetic coils 420 may be adapted for location or placement on the inside, outside or in a pocket of shirt 400, and may be integrated into or removably coupled thereto.
  • One or more cables or wires 450 may be provided to couple each of the coils 420 to an electronics box 460, which may house the remaining components of the MFT therapy system 100 of Figure 1 such as APMFG 110, controller 130, power supply 150, and in some embodiments interface 140.
  • APMFG 110, controller 130, power supply 150, or interface 140 may be provided separately from the electronics box 460.
  • a separate interface 140 may be provided as a mobile phone app that communicates with one or more of APMFG 110, controller 130, power supply 150, etc.
  • Such an app-based interface may also provide information on the treatment therapy to the patient or a healthcare provider (e.g., displaying how long the therapy has been applied, whether a magnetic field is currently being applied to the target tissue from each of the coils 420, the frequency and/or field strength of the currently-provided magnetic fields, remaining battery life, etc.).
  • a healthcare provider e.g., displaying how long the therapy has been applied, whether a magnetic field is currently being applied to the target tissue from each of the coils 420, the frequency and/or field strength of the currently-provided magnetic fields, remaining battery life, etc.
  • Figure 5 illustrates a neck cuff or collar 500 that acts as a retaining element for one or more AP electromagnetic coils 520 for applying one or more magnetic fields to the treatment of cancer or other rapidly-dividing cells in a target tissue in a patient’s neck area, including without limitation esophageal cancer, laryngeal cancer, etc.
  • AP electromagnetic coils 520 may be similar to coils 120 described in Figure 1 , but may be adapted for placement in neck cuff or collar 500 based on the target treatment area or tissue. This may include changes in the size, geometry, or other characteristics to enable effective placement in neck cuff 500 and for treatment of the particular target tissue.
  • Neck cuff or collar 500 preferably includes a securing and/or adjustment tab 530 (e.g., Velcro) to adjust the cuff or collar to the patient’s size and to secure it in a fixed position relative to the patient’s neck.
  • a neck scarf may be used as a retaining element.
  • AP electromagnetic coils 520 may be adapted for location or placement on the inside, outside or in a pocket of neck cuff or collar 500, and may be integrated into or removably coupled thereto.
  • One or more cables or wires 550 may be provided to couple each of the coils 520 to an electronics box 560, which may house the remaining components of the MFT therapy system 100 of Figure 1 such as APMFG 110, controller 130, power supply 150, and in some embodiments interface 140.
  • APMFG 110, controller 130, power supply 150, or interface 140 may be provided separately from the electronics box 560.
  • a separate interface may be provided as a mobile phone app that communicates with one or more of APMFG 110, controller 130, power supply 150, etc.
  • Such an app-based interface may also provide information on the treatment therapy to the patient or a healthcare provider (e.g., displaying how long the therapy has been applied, whether a magnetic field is currently being applied to the target tissue from each of the coils 520, the frequency and/or field strength of the currently-provided magnetic fields, remaining battery life, etc.).
  • a healthcare provider e.g., displaying how long the therapy has been applied, whether a magnetic field is currently being applied to the target tissue from each of the coils 520, the frequency and/or field strength of the currently-provided magnetic fields, remaining battery life, etc.
  • Figure 6 illustrates a bandage 600 that acts as a retaining element for one or more magnetic coils 620 for applying one or more magnetic fields to the treatment of cancer or other rapidly-dividing cells in a target tissue anywhere on the body.
  • Magnetic coils 620 may be similar to those described in Figure 1 , but may be adapted for placement in bandage 600 based on the target tissue. This may include changes in the size, geometry, or other characteristics to enable effective placement in bandage 600 and for treatment of any of a variety of different target tissues.
  • Magnetic coils 620 may be adapted for location or placement on the inside, outside or in a pocket of bandage 600, and may be integrated into or removably coupled thereto.
  • One or more cables or wires 650 may be provided to couple each of the coils 620 to an electronics box 660, which may house the remaining components of the MFT therapy system 100 of Figure 1 such as APMFG 110, controller 130, power supply 150, and in some embodiments interface 140.
  • APMFG 110, controller 130, power supply 150, or interface 140 may be provided separately from the electronics box 560.
  • a separate interface may be provided as a mobile phone app that communicates with one or more of APMFG 110, controller 130, power supply 150, etc.
  • Such an app-based interface may also provide information on the treatment therapy to the patient or a healthcare provider (e.g., displaying how long the therapy has been applied, whether a magnetic field is currently being applied to the target tissue from each of the coils 620, the frequency and/or field strength of the currently-provided magnetic fields, remaining battery life, etc.).
  • a healthcare provider e.g., displaying how long the therapy has been applied, whether a magnetic field is currently being applied to the target tissue from each of the coils 620, the frequency and/or field strength of the currently-provided magnetic fields, remaining battery life, etc.
  • the retaining element may also provide additional features to enable the MFT therapy to be conveniently delivered to the target body area or tissue.
  • the retaining element may have integrated magnetic coils 120, APMFG 110, and controller 130, either as separate items in the retaining element or as a single unit.
  • a wire (not shown) may be provided to couple the power supply to one or more of the APMFG 110, coils 120, controller 130, and interface 140.
  • the power supply 150 provides power to the controller, which includes circuitry (e.g., rectifiers, converters, transformers, etc.) to modify the electrical power received from the power supply to provide electrical power to controller 130, which in turn distributes power to the APMFG 110, AP electromagnetic coils 120, and interface 140.
  • a power supply e.g., a battery
  • the patient e.g., in a pocket in the patient’s trousers or a jacket.
  • the MFT therapy may be provided to a patient in combination with one or more other therapies such as an anti-cancer drug, radiation therapy, or TTF therapy (e.g., therapy as described in US 6,868,289 or US 8,019,414).
  • the MFT therapy system 100 preferably permits the other (i.e., non-MFT) therapy to be provided at a lower dosage than would be administered in the absence of the MFT therapy to the target body area or tissue, or at a reduced frequency than would be administered in the absence of the MFT therapy, or both.
  • the co-therapy applied with the MFT therapy may be a drug selected from a chemotherapy drug, a hormone receptor drug, targeted therapy drugs, immunotherapy, angiogenesis inhibitor drugs, a checkpoint inhibitor drug, and a HER2 receptor drug.
  • the co-therapy may be a radiation therapy selected from an internal radiation therapy and an external beam radiation therapy.
  • the co-therapy applied with the MFT therapy may be a TFT therapy involving the application of electrical fields to the target tissue.
  • the combination therapy may comprise administering MFT therapy with an anti-cancer drug, radiation, or TTF therapy either simultaneously or sequentially.
  • an optional magnetic field shield (not shown) may be provided to shield the non-target areas from the effects of the magnetic fields.
  • highly localized shields may be provided to shield specific structures within the target body area of the patient, such as specific blood vessels or organ structures that are adjacent to the target rapidly-dividing cells.
  • TTF therapies begin to inhibit tumor cell growth at a field strength of about 100 V/m at frequencies of 50 - 250 kHz.
  • MFT therapies showed surprising results with a similar inhibition of tumor cell growth as reported for TTF therapies but at a fraction (e.g., less than 3%) of typical TTF therapy electric field strengths.
  • the experimental results discussed hereinafter suggest that efficacious frequencies for TTF and MFT therapies are different as well.
  • Mouse melanoma cells (B16F10 cell line, obtained from the University of California-Berkeley) were incubated in Dulbecco's Modified Eagle Medium (DMEM) in 36 middle wells (5.0 mm diameter) of a 96 well plate for 24 hours at 37°C. The cells in each of the 36 treatment wells were then exposed for 24 hours to an alternating magnetic field at a frequency of 150 kHz and a magnetic field strength of approximately 0.8 mT using a Helmholtz coil, maintained at a temperature of 37°C. Control wells were not exposed to the alternating magnetic field and were incubated at 37°C for the same time period.
  • DMEM Dulbecco's Modified Eagle Medium
  • Figures 7A and 7B are illustrative of the differences between typical control and treatment wells. Controls exhibited a significantly higher cell count per well as shown by gross comparison. In addition, control cells maintained a typically angular morphological structure as indicating in Figure 7A. Magnetic field-treated cells showed significantly decreased cell count and in addition demonstrated rounded morphology indicating cell stress, as shown in Figure 7B.
  • Figure 8 provides a bar chart comparison summarizing the results of treatments performed on two different plates with 36 wells in each plate. Normalizing the cell counts of the control wells as 100, the treated cells showed a reduction of approximately 31 %.
  • treatment by magnetic fields and electrical fields e.g., TTF therapy
  • coils vs electrodes and generating primarily magnetic vs. electric fields are fundamentally different (e.g., using coils vs electrodes and generating primarily magnetic vs. electric fields)
  • it is possible to calculate the strength of the induced electric field from the coils used in Experiment 1 using the equation r dB F - n c 2 dt
  • Equation 1 Using equation 1 yields a maximum inducted voltage of 2.34 V/m, or less than 3% of the electric field strengths reported as required for inhibitory activity in TTF therapy. Because Experiment 1 indicates that MFT therapy exhibits effects on cancer cells at such a small fraction of the electrical field strength of TTF therapy, it enables therapies having significant advantages over TTF therapies, including without limitation ambulatory therapies that allow patients to continue many ordinary day-to-day activities without interruption, and minimal encumbrance or burden.
  • MFT therapies Part of the advantage of MFT therapies over TTF therapies stems from the different hardware configurations of the two systems. While TTF therapies use insulated (e.g., ceramic coated) electrodes, the use of coils instead of electrodes in MFT therapy confers a number of benefits. Because MFT therapy coils — in contrast to the insulated electrodes of TTF therapies — do not need to be in direct contact with the body, MFT coils can be separated from target issue by one or more clothing layers (e.g., a garment or undergarment). By applying magnetic fields through clothing, MFT therapies provide increased patient comfort and a less cumbersome patient experience.
  • clothing layers e.g., a garment or undergarment
  • MFT therapies can be implemented with significantly less risk to the patient than TTF therapies involving electrodes.
  • the use of coils instead of electrodes results in only a de minimis induced electrical current during MFT therapies, and thus the risk of electrical shorting and consequent uncontrolled heating of patient body tissue is negligible.
  • MFT therapies involve coils that can be made relatively small and with minimal current flow through the patient’s body
  • systems for MFT therapies can allow long treatment periods to target cancer and other hyperproliferating cells with little inconvenience to the patient.
  • mice were kept in plastic cages with free access to food and water and under standardized light/dark cycle conditions. Mice were inoculated subcutaneously with 5x10 5 4T1 murine breast carcinoma cells and one week later were randomly divided into the control and therapy groups.
  • mice in the therapy group were exposed to therapy on the same day as they were randomized, which is designated as do.
  • the length, width, and height of the tumor were measured with a fine caliper twice each week for the study duration.
  • the tumor volume was expressed as 0.5 x length x width x height of the tumor.
  • Antitumor activity was evaluated over the study duration, and the tumor growth inhibition ratio (IR) was calculated at the study end date (dt) using the equation
  • Figure 9 shows the measured tumor volume over the study duration for the control and therapy groups. The inhibition ratio (IR) in the therapy group was 25.9%.
  • Figure 9 suggests that the cancerinhibiting effects of MFT therapy may be measurable as early as 4 days after the start of the therapy, and that the effects of the therapy become more significant over time, as illustrated by the difference in tumor volume at the end of the study (day 31 ).
  • Therapy was provided as an alternating polarity magnetic field having a field strength of 0.8 - 1 mT (all therapy groups) and frequencies of 26 kHz ( ⁇ 1 khz), 51 khz ( ⁇ 1 khz) and 97khz ( ⁇ 1 khz), respectively, for therapy groups 1 , 2 and 3.
  • Therapy was provided continuously for 28 days, at which time the animals were sacrificed. The tumor was measured twice every week and the tumor volume was estimated form these measurements.
  • Figure 12 shows the tumor growth across all 4 groups.
  • Figure 12 suggests that, at least for the 4T1 murine breast carcinoma cells under study, there appears to be a frequency-dependent response, with greatest efficacy at approximately 50 kHz.
  • therapies for various cancer cell types may be characterized by a frequency distribution that describes an optimal therapy response. It is also hypothesized that an optimum frequency or frequency distribution may be experimentally determinable for different types of cancer cells, and that therapy may be provided as a single (optimum) frequency that provides the maximum inhibition ratio, or as a frequency distribution including the optimum frequency.
  • alternating polarity magnetic fields at multiple individual frequencies or multiple overlapping or non-overlapping frequency distributions varying in a random, non-random or time-dependent distribution (e.g., a Gaussian distribution) may be used to treat cancers of various types.
  • a random, non-random or time-dependent distribution e.g., a Gaussian distribution
  • mice weight was typically measured twice a week throughout Experiments 2 and 3, and there was no significant difference in mice weight between the therapy and control arms at therapy end (df).
  • the invention comprises a method for treating cancer cells by applying to a target body area or body region an alternating polarity magnetic field having a field strength of from 0.05 - 5 mT and a frequency within the range of 1 kHz - 100 kHz, where the magnetic field selectively affects the cancer cells to achieve at least one of damaging the cancer cells, inhibiting their growth, reducing tumor size, inhibiting angiogenesis, eliciting an immune response to the cancer cells, increasing tumor immunogenicity, decreasing immunosuppressive activity of the cancer cells, recruiting one of antigen-presenting cells and immune effector cells to the tumor microenvironment, or preventing metastasis of the cancer cells.
  • the therapy leaves non-cancer cells substantially unharmed.
  • the method may comprise applying to cancer cells in a target body area or region an alternating polarity magnetic field having a frequency withing a frequency range selected from 0.2-400 kHz, 0.5-300 kHz, 1 -200 kHz, 5-150 kHz, 10-100 kHz, and 25-100 kHz.
  • the AP magnetic fields may be applied to the body of the patient using one or more coils, the operation of which may be controlled by a controller that controls the parameters of the magnetic field therapy, which may include frequency, field strength, duty cycle, etc.
  • the controller may also control the operation of various sensors such as body parameter sensors (e.g., temperature sensors, neural activity sensors, etc.) and sensors adapted to detect one or more effects of the MFT therapy, such as ultrasound sensors to detect tumor size, e.g., ultrasound waves delivered to the patient using one or more ultrasound generating elements such as a piezoelectric ultrasound element.
  • body parameter sensors e.g., temperature sensors, neural activity sensors, etc.
  • sensors adapted to detect one or more effects of the MFT therapy such as ultrasound sensors to detect tumor size, e.g., ultrasound waves delivered to the patient using one or more ultrasound generating elements such as a piezoelectric ultrasound element.
  • Figure 10 illustrates a system according to one embodiment of the invention using ultrasound to image the cancer cells in the target body area simultaneously or sequentially with the delivery of a magnetic field tumor therapy (MFTT).
  • MFTT magnetic field tumor therapy
  • Certain components of the system are similar to like components in the system of Figure 1 .
  • coils 1020, magnetic field generator 1010, magnetic field controller 103 (including timing control module 1012, frequency control module 1014, and magnetic field strength control module 1016), interface 1040, and power supply 1050 provide similar functions to those of coils 120, magnetic field generator 110, magnetic field controller 130, timing control module 112, frequency control module 114, magnetic field strength control module 116, interface 1040, and power supply 1050 of Figure 1.
  • discussion of those elements of Figure 10 is simplified herein.
  • the invention comprises delivering an MFT therapy system comprising at least one body-worn electromagnetic coil 1120 and one or more ultrasound elements 1060 coupled to the patient (e.g., in an article of clothing and/or an ultrasound sensor retaining element), and periodically imaging the tumor using the ultrasound elements 1060 (which may comprise, e.g., ultrasound delivery elements and ultrasound sensors).
  • the system may comprise an ultrasound generator 1065 coupled to the ultrasound elements (e.g., piezoelectric probes and sensors to detect reflected ultrasound energy from body tissue) and to a controller 1070 having software to analyze the ultrasound data and/or images and using the images to interrupt, restart, or adjust therapy, thereby providing a closed-loop therapy.
  • ultrasound controller 1070 includes a timing control module 1072 for controlling the timing of ultrasound images (e.g., obtaining images at a frame rate of 1 -500 frames per second for a defined time periods and/or timepoints (such as once every 10 minutes, 5 images every 30 minutes, etc.), an ultrasound probe control module 1074 for controlling the application of ultrasound energy to the target body area and the detection of reflected ultrasound energy from the target via ultrasound elements 1060, and an ultrasound image generation and analysis module 1076 for processing the ultrasound energy reflected from the patient’s body to produce ultrasound images of the cancer cells (e.g., a tumor).
  • a timing control module 1072 for controlling the timing of ultrasound images (e.g., obtaining images at a frame rate of 1 -500 frames per second for a defined time periods and/or timepoints (such as once every 10 minutes, 5 images every 30 minutes, etc.)
  • an ultrasound probe control module 1074 for controlling the application of ultrasound energy to the target body area and the detection of reflected ultrasound energy from the target via ultrasound elements 1060
  • the MFT therapy system of Figure 10 further comprises a power supply 1080 and one or more interfaces 1090 for a user (e.g., a patient and/or caregiver), although it will be appreciated that power supply 1080 may be combined with power supply 1050 into a single power supply (which may comprise one or more of a battery or standard interface to an AC system such as a wall socket).
  • interfaces 1090 and 1040 may be combined into a single interface for a particular user (e.g., patient, caregiver) that provides information on both the MFT therapy and the ultrasound portion of the system (e.g., providing ultrasound images for display to the patient or caregiver).
  • a particular user e.g., patient, caregiver
  • the ultrasound portion of the system e.g., providing ultrasound images for display to the patient or caregiver.
  • the system of Figure 10 may provide a closed-loop therapy in which the magnetic field controller 1030 uses ultrasound data (e.g., ultrasound images from image generation/analysis module 1076) to automatically change a parameter of the MFT therapy (e.g., the magnetic field strength, the magnetic field frequency, duty cycle, on time, off time, etc.).
  • the magnetic field controller may use the ultrasound data to take additional actions, such as to communicate a message to the patient or a caregiver, to recommend a change in a therapy parameter of the MFTT or a drug therapy, to turn the MFTT therapy on or off, or to turn an adjunctive therapy (e.g., a TTF therapy or a nanoparticle hyperthermia therapy) on or off, etc.
  • ultrasound data e.g., ultrasound images from image generation/analysis module 1076
  • the magnetic field controller may use the ultrasound data to take additional actions, such as to communicate a message to the patient or a caregiver, to recommend a change in a therapy parameter of the MFTT or a drug therapy, to turn
  • ultrasound images of cancer cells and/or a tumors in the target body area may be automatically captured (e.g., using ultrasound elements 1060, ultrasound generator 1065, and ultrasound image generation and analysis module 1076) and transmitted (e.g., automatically via a body-worn computing device coupled to a wireless or cloud-based network) to one or both of the patient and a healthcare provider, who may analyze the images and transmit instructions to a body-worn therapy system to change one or more therapy parameters.
  • the invention comprises delivering an MFTT using a system comprising at least one body-worn electromagnetic coil and one or more heating or cooling elements to deliver a magnetic field therapy to a target body area or region of the patient simultaneously or sequentially with a hyperthermia or hyperthermia therapy to heat or cool cancer cells (e.g., a tumor).
  • a hyperthermia or hyperthermia therapy to heat or cool cancer cells (e.g., a tumor).
  • Hyperthermia therapy in which tumors are heated, has been shown to inhibit tumors. Without being bound by theory, it is hypothesized that providing both MFTT and hyperthermia therapy may result in more efficacious therapies for treating cancer cells.
  • one or more ultrasound delivery elements 1060 e.g. , piezoelectric elements, not shown
  • the ultrasound elements may be used for both imaging and for providing hyperthermia elements. In other embodiments, separate ultrasound elements by be provided for imaging and for hyperthermia therapy.
  • the invention comprises providing an MFTT to a patient and using nanoparticles to apply a hyperthermia therapy to a target body area or region comprising cancer cells.
  • nanoparticles may be delivered to a tumor, and heated by the MFTT system by applying one or more of an alternating polarity magnetic field, ultrasound energy, or another heat source (not shown) to the nano particles.
  • the alternating polarity magnetic field used to heat the nanoparticles may have a field strength and frequency as previously described, or may have different field strength and frequency parameters than those used to provide the MFT therapy.
  • MFTT operates in at least one mode of action by favorably altering the tumor microenvironment (TME) and can work synergistically with other anti-cancer therapies, including without limitation immunotherapies.
  • TME tumor microenvironment
  • Tumor microenvironment or TME refers to the area (which may more accurately comprise a volume of tissue in the patient’s body) surrounding the cancer cells/tumor that encompasses surrounding blood vessels, fibroblasts, immune cells, signaling molecules, the extracellular matrix, resident and infiltrating host cells, secreted factors, proteins, tumor vasculature and lymphatics, pericytes and sometimes adipocytes.
  • the TME is a complex ecosystem where subtle interactions profoundly impact tumor progression by influencing processes that lead to tumor eradication, increased metastasis, or the establishment of dormant micrometastases.
  • the tumor microenvironment can also shape therapeutic responses and resistance, justifying the recent impetus to target components of the tumor microenvironment, which is best exemplified by the success of immunotherapies in the clinic.
  • cancer cells may be present in the body outside of a tumor
  • the more generic term “cancer microenvironment” may also be used to clarify that methods and systems of the present invention may be used to modulate one or more aspects of the environment surrounding cancer cells.
  • the terms are interchangeable, and “cancer microenvironment,” “CME,” “tumor microenvironment,” and “TME” as used herein are each intended to refer to the area as described above, regardless of whether the cancer cells are part of a tumor.
  • Immune cells in the TME are fundamental determinants of the invasive and metastatic activity of the cancer cells, and of the tumor’s fate.
  • a dominant anti-tumor immune cell is the CD8+ T lymphocyte, which can recognize tumor cells in an antigenspecific manner and secrete cytotoxic molecules to kill them directly.
  • CD8+ T lymphocyte A dominant anti-tumor immune cell
  • Studies have shown that cancer patients with high CD8+ levels have significantly better recurrence-free survival and overall survival rates.
  • CD8+ T cells must be primed and “educated” by antigen presenting cells (APCs), which are dendritic cells.
  • APCs antigen presenting cells
  • MDSCs myeloid-derived suppressor cells
  • TME myeloid-derived suppressor cells
  • MDSCs are a heterogeneous population of various types of immature myeloid cells that recent studies have shown to cause immune suppression and cancer progression through a variety of mechanisms. Studies have established that elevated MDSC levels in cancer patients are associated with shorter overall patient survival and poor disease-free survival and/or recurrence-free survival.
  • cells such as the CD8+ T cells and MDSCs show a consistent anti-cancer or pro-cancer activity
  • other immune cells demonstrate plasticity in the MTE, and are capable of either tumor-promoting or tumor-inhibiting activity, depending upon other factors in the TME.
  • M1 macrophages
  • M2 can promote immunosuppression, again depending upon the TME.
  • MFT therapy was able to disrupt cell growth in multiple cell lines, but also that the outcome depends on exposure frequency and field strength. In particular, exposure above a certain frequency of approximately 500 kHz does not result in a statistically significant reduction in the rate of cancer cell growth.
  • FIG. 11 summarizes the results of a number of experiments treating the MB231 cell line of triple negative breast cancer (TNBC), a highly aggressive cancer, and for the B16F10 melanoma cell line.
  • TNBC cells do not have estrogen or progesterone receptors and produce little HER2 protein. Consequently, treatment options for these cancers are limited, primarily to chemotherapies. Moreover, though TNBC may respond to chemotherapy initially, recurrences of TNBC after such response is more frequent that in other breast cancers.
  • Each row in the table of Figure 11 shows the results of a series of experiments in which a number of wells having the cell line were exposed to MFT therapy at a particular frequency and field strength.
  • 20 wells on 96 well plates of the MB231 TNBC cells were exposed for 4 days to magnetic fields at a frequency of 525 kHz and 78 pT (0.078 mT).
  • MFT therapy-exposed cells showed similar cell growth as control cells.
  • 20 wells on 96 well plates of the MB231 TNBC cells were exposed for 4 days to magnetic fields at a frequency of 745 kHz and 36 pT (0.036 mT).
  • MFT therapy-treated cells showed similar cell growth as control cells. Both lines 1 and 2 suggest that MFT at frequencies exceeding approximately 500 kHz might not be effective.
  • Line 4 of Table 11 indicates that the MFT-exposed cells showed a statistically significant reduction in cell growth compared to the control group. This corresponded to a 31 % reduction of the MFT-exposed cells vs. controls, with a statistically significant p-value of less than 0.0001 .
  • MFT therapy has shown promising results in initial preclinical studies on two different animal models covering breast (4T1 ) and colon cancers (CT26), including inhibiting tumor growth rate ( Figures 12 and 13), and reducing tumor metastasis to distant organs ( Figure 14). These studies also indicate that using MFT therapy in conjunction with an immunotherapy (checkpoint inhibitor anti-PD1 drug) further improves therapy efficacy.
  • Figure 12 is a graph showing the increase in tumor volume over a 30-day period for breast cancer cells (4T1 cell line) in an animal model involving in which control and MFT therapy-treated animals were tested as described in connection with Experiments 2 and 3 above. Treatment animals were exposed continuously for 30 days to an AP magnetic field at 26 kHz, 50 kHz, or 100 kHz frequencies and a field strength of 1 mT. Control animals were not exposed to magnetic field therapy. As shown in the graph of Figure 12, animals treated with MFT at frequencies of 26 and 50 kHz showed a diminished growth in tumor volume at sacrifice at 30 days, while animals treated at a frequency of 100 kHz showed an increase in tumor growth relative to control. This study suggests that the effects of MFT are dependent upon frequency.
  • Figure 13 is a graph showing the increase in tumor volume over a 30-day period for colon cancer cells (CT6 cell line) in an animal model comparing the combination of MFT therapy (50 kHz, 1 mT) and a checkpoint inhibitor anti-PD1 drug (an immunotherapy drug) to the checkpoint inhibitor drug alone, and to isotype lgG2a and control (no exposure to MFT or any drug) animals.
  • MFT therapy 50 kHz, 1 mT
  • an immunotherapy drug an immunotherapy drug
  • the graph of Figure 13 shows that animals treated with immunotherapy showed a diminished growth in tumor volume after 30 days compared to control and isotype animals.
  • animals treated with the combination of MFT therapy and the immunotherapy drug show even further diminished growth compared to the immunotherapy drug alone, suggesting a synergistic effect of MFT therapy and immunotherapy.
  • FIG. 14 is a bar graph showing relative metastasis of breast cancer cells (4T1 cell line) in an animal lung model.
  • MFT therapy 50 kHz, 1 mT
  • the anti-PDF checkpoint inhibitor drug were each used alone in separate animals, and in a third cohort animals were treated both MFT therapy and the anti-PDF checkpoint inhibitor drug.
  • balb/c female mice kept in plastic cages with free access to food and water under standardized light/dark cycle conditions were subcutaneously inoculated with 5x105 4T1 murine breast carcinoma cells, then randomly divided into 4 groups one week later. The animals were sacrificed 28 days after initiation of therapy and the metastatic lung metastases were visually counted.
  • the first bar in Figure 14 shows the average number of lung metastasis nodules in the control group.
  • the second bar shows the number of lung metastasis nodules in animals exposed to MFT therapy alone, and shows a significant reduction compared to control animals.
  • the third bar shows that animals treated with the anti-PDF checkpoint inhibitor drug alone show a decrease in metastatic lung nodules relative to control but not as significant as MFT therapy alone.
  • the fourth bar shows that animals treated with both MFT therapy and immunotherapy exhibited a dramatic decrease in metastatic lung nodules relative to control, MFT alone, and immunotherapy alone.
  • Figure 14 shows that MFT therapy and immunotherapy appear to be strongly synergistic for inhibiting metastasis for at least some cancers.
  • Figure 15 is a bar graph showing CD8+ T cells as a percentage of all cells in the TME in a breast cancer cell (4T1 cell line) animal model. Concentrations of CD8_ cells were measured using flow cytometry. As stated earlier the CD8+ T cells exhibit an anticancer effect. Treated animals were exposed to either MFT therapy (50 kHz, 1 mT) alone; the checkpoint inhibitor anti-PD1 drug alone; or both MFT therapy and the checkpoint inhibitor anti-PD1 drug. The first bar shows control animals having a very low presence of CD8+ cells.
  • Figure 16 is a bar graph showing the relative concentration of MDSC cells as a percentage of all cells in the TME in the same breast cancer animal model as Figure 15 (4T1 cell line). MDSCs, as previously noted, correlate with poor patient survival. As in Figure 15, treated animals were exposed to either MFT therapy (50 kHz, 1 mT) alone; the checkpoint inhibitor anti-PD1 drug alone; or both MFT therapy and the checkpoint inhibitor anti-PD1 drug.
  • MFT therapy 50 kHz, 1 mT
  • the checkpoint inhibitor anti-PD1 drug alone
  • both MFT therapy and the checkpoint inhibitor anti-PD1 drug both MFT therapy and the checkpoint inhibitor anti-PD1 drug.
  • Control animals showed, as expected, a relatively high concentration of MDSCs, as did animals treated with the checkpoint inhibitor anti-PD1 drug alone (Bar 3).
  • Animals exposed to MFT therapy 50 kHz, 1 mT) showed a significantly diminished relative concentration of MDSCs than control or drug-treated animals.
  • Animals treated with both MFT therapy and the checkpoint inhibitor anti-PD1 drug showed an intermediate concentration of MDSCs, between the MFT therapy animals and the control or drug- treated animals.
  • Figured 15 and 16 suggest that MFT shows strong enhancement of certain anti-cancer cells and/or reduction of pro-cancer cells in the TME, an effect that is somewhat reduced by co-therapy with the drug.
  • Figure 17 is a bar graph showing the relative concentration of dendritic cells (DCs) as a percentage of all cells in the TME in the same breast cancer animal model as Figures 15 and 16 (4T1 cell line). DCs exhibit an anti-cancer/antitumor effect and correlate with improved patient survival. As in Figures 15 and 16, treated animals were exposed to either MFT therapy (50 kHz, 1 mT) alone; the checkpoint inhibitor anti-PD1 drug alone; or both MFT therapy and the checkpoint inhibitor anti-PD1 drug.
  • MFT therapy 50 kHz, 1 mT
  • the checkpoint inhibitor anti-PD1 drug alone
  • both MFT therapy and the checkpoint inhibitor anti-PD1 drug both MFT therapy and the checkpoint inhibitor anti-PD1 drug.
  • Control animals showed a relatively low concentration of dendritic cells.
  • Animals treated with MFT therapy alone showed an improvement in the concentration of dendritic cells relative to control animals.
  • Bar 3 shows a small increase in dendritic cell concentration relative to MFT-treated animals in animals treated with immunotherapy alone.
  • Animals treated with both MFT therapy and the checkpoint inhibitor anti-PD1 drug showed a further increase in the concentration of dendritic cells relative to control, MFT-treated animals, and drug-treated animals.
  • the present invention relates to the subject matter of the following numbered paragraphs.
  • a method of treating cancer cells in a target body area of a patient comprising: providing a magnetic field therapy system comprising: an alternating polarity (AP) magnetic field generator; one or more AP electromagnetic coils coupled to the AP magnetic field generator, wherein the one or more AP electromagnetic coils are energized by an electrical signal from the AP magnetic field generator to generate an AP magnetic field having at least a first frequency and a first field strength; and a controller to control at least one of the first frequency and the first field strength of the AP magnetic field generated by the one or more AP electromagnetic coils; coupling the one or more AP electromagnetic coils to the target body area; generating an AP magnetic field having a frequency of 0.1 -500 kHz and a field strength of 0.1 -500 kHz and a field strength of 0.2-5 mT using the one or more AP electromagnetic coils; applying the generated AP magnetic field to the target body area using the one or more AP electromagnetic coils, wherein the AP magnetic field selectively
  • generating an AP magnetic field comprises generating an AP magnetic field having a frequency within a frequency range selected from 0.2-400 kHz, 0.5-300 kHz, 1 -200 kHz, 5-150 kHz, 10-100 kHz, and 25-100 kHz; and a field strength within a field strength range selected from of 0.2-5 mT, 0.2-4.0 mT, 0.4-3.0 mT, 0.5-2.0 mT, and 0.1 -1.6 mT.
  • 104 The method of numbered paragraph 101 , wherein the AP magnetic field selectively affects the cancer cells to achieve at least one of damaging the cancer cells, inhibiting the growth of the cancer cells, reducing tumor size, inhibiting angiogenesis, eliciting an immune response to the cancer cells, increasing tumor immunogenicity, decreasing immunosuppressive activity of the cancer cells, recruiting one of antigen- presenting cells and immune effector cells to the tumor microenvironment, or preventing or decreasing metastasis of the cancer cells, while leaving non-cancer cells substantially unharmed.
  • coupling the one or more AP electromagnetic coils to the target body area comprises using a retaining element to maintain the AP electromagnetic field generator in a desired position proximate to the target body area, and wherein the retaining element is selected from a garment and a bandage.
  • cancer cells are brain cancer cells selected from astrocytomas, glioblastoma multiforme, meningioma, and pituitary tumors
  • the retaining element is a head covering selected from a hat, a helmet, and a garment head covering.
  • the cancer cells comprise one of: breast cancer cells, and the retaining element is a wearable garment selected from a bra, a shirt, a vest, and a jacket; a cancer selected from lung cancer, lung carcinoid tumors, thymic malignancies, tracheal tumors, pancreatic cancer, liver cancer, stomach cancer, kidney cancer, ovarian cancer, colon cancer and rectal cancer, and the retaining element is a wearable garment selected from a bra, a shirt, a vest, and a jacket; a lower-body cancer selected from prostate cancer, ovarian cancer, colon cancer, and rectal cancer, and the retaining element is an undergarment; a skin cancer, and the retaining element is one of an adhesive bandage and a nonadhesive bandage; a throat, thyroid, mouth, nose, and salivary gland cancer, and the retaining element is selected from a neck cuff, a neck collar, and a scarf; and a brain
  • coupling the one or more AP electromagnetic coils to the target body area comprises coupling a plurality of coils to the target body area, wherein each coil in said plurality of coils is oriented so as to apply a desired AP magnetic field distribution in the target body area.
  • coupling the one or more AP electromagnetic coils to the target body area comprises coupling a plurality of AP electromagnetic coils to the target body area, the method further comprising: selecting one or more coils from the plurality of coils to activate to apply the AP magnetic field based on one of: the result of an imaging procedure selected from an MRI scan, a CT scan, a PET scan, and an X-ray, and a pathological analysis of the cancer cells selected from a microscopic analysis of a cell biopsy and a chemical test performed on the cancer cells; and wherein applying the generated AP magnetic field to the target body area comprises applying the generated AP magnetic field to the target body area using the selected one or more AP electromagnetic coils.
  • applying the generated AP magnetic field to the target body area comprises one or more of: applying the AP magnetic field continuously for a first treatment period; applying the AP magnetic field intermittently for a second treatment period in alternating on time periods in which the AP magnetic field is applied to the target body area, followed by off time periods in which the AP magnetic field is not applied to the target body area; applying the AP magnetic field intermittently for one or more circadian treatment periods based on circadian rhythms of the patient; and applying the AP magnetic field intermittently for one or more third treatment periods at defined times of day.
  • applying the generated AP magnetic field to the target body area comprises one or more of: applying an AP magnetic field having a single frequency of 0.5 - 150 kHz to the target body area; applying an AP magnetic field for a defined time period having a frequency of 0.5 - 150 kHz that varies in a defined pattern; and applying an AP magnetic field having multiple simultaneous frequencies of 0.5 - 150 kHz.
  • applying an AP magnetic field for a defined time period having a frequency of 0.5 - 150 kHz that varies in a defined pattern comprises: applying an AP magnetic field in which the frequency changes at a defined rate; applying an AP magnetic field in which the frequency varies in a Gaussian distribution in one or more ranges within the range of 0.5 - 150 kHz; and applying an AP magnetic field in which the frequency varies in a non-Gaussian distribution in one or more ranges within the range of 0.5 - 150 kHz.
  • applying the generated AP magnetic field to the target body area comprises applying the generated AP magnetic field to the target body area one of continuously or intermittently according to a defined duty cycle comprising alternating on time periods in which the generated AP magnetic field is applied to the target body area followed by off time periods in which no magnetic field is applied to the target body area, the method further comprising: coupling at least one ultrasound sensing element to the patient; obtaining at least one ultrasound image of the target body area using the ultrasound sensing element; and taking a responsive action based on the at least one ultrasound image, wherein the at least one responsive action is selected from: analyzing the at least one ultrasound image; terminating the applying of the AP magnetic field to the target body area; adjusting at least one of the field strength, the frequency, and the defined duty cycle of the AP magnetic field applied to the target body area; transmitting the at least one ultrasound image to a healthcare provider; and transmitting the at least one ultrasound image to the patient.
  • applying the generated AP magnetic field to the target body area comprises applying the generated AP magnetic field to the target body area one of continuously or intermittently according to a defined duty cycle to the target body area for a first treatment period, the method further comprising: coupling at least one of a heating element and a cooling element to the target body area of the patient; and providing a thermal action selected from heating the at least one target body area to provide a hyperthermia to the cancer cells and cooling the target body area to provide a hypothermia to the cancer cells during at least a portion of the first treatment period.
  • generating an AP magnetic field comprises generating an AP magnetic field having a frequency within a frequency range selected from 0.2-400 kHz, 0.5-300 kHz, 1 -200 kHz, 5-150 kHz, 10-100 kHz, and 25-100 kHz, and a field strength within a field strength range selected from 0.2- 5 mT; 0.2-4 mT; 0.4-3 mT; 0.5-2 mT. and 0.1 -1.6 mT.
  • applying an AP magnetic field to the target body area comprises applying to the target body area an AP magnetic field comprising a series of pulse bursts, wherein each pulse in the pulse burst occurs at a pulse frequency of 0.01 -1000 Hz and has a pulse duration of 0.1 -5000 msec, and wherein each pulse in the pulse burse comprises a frequency of 0.1 - 500 kHz and a field strength of 0.1 -5 mT
  • a method of treating cancer cells in a target body area of a patient comprising: providing a magnetic field therapy system comprising: an alternating polarity (AP) magnetic field generator; one or more AP electromagnetic coils coupled to the AP magnetic field generator, wherein the one or more AP electromagnetic coils are energized by an electrical signal from the AP magnetic field generator to generate an AP magnetic field having at least a first frequency and a first field strength; and a controller to control at least one of the first frequency and the first field strength of the AP magnetic field generated by the one or more AP electromagnetic coils; coupling the one or more AP electromagnetic coils to the target body area; generating an AP magnetic field having a first frequency of 0.1 -500 kHz and a field strength of 0.2-5 mT using the one or more AP electromagnetic coils; applying the generated AP magnetic field to the target body area using the one or more AP electromagnetic coils, wherein the AP magnetic field modifies the cancer microenvironment to achieve at least one of:

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Abstract

L'invention concerne des systèmes et une méthode de destruction ou d'inhibition de cellules cancéreuses et d'autres cellules à division rapide qui consistent à appliquer des champs magnétiques AP ayant une fréquence définie de 5 Hz à 500 kHz et une intensité de champ de 0,1 à 5000 μΤ sur une zone corporelle cible atteinte par le cancer ou d'autres cellules à division rapide, et à modifier le cancer ou le micro-environnement tumoral pour augmenter la présence de cellules éliminant le cancer ou diminuer la présence de cellules favorisant le cancer. Dans divers modes de réalisation, les systèmes et les méthodes peuvent consister à ajuster la thérapie sur la base d'une imagerie ultrasonore des cellules cancéreuses pendant l'application des champs magnétiques AP ou pendant un arrêt d'application des champs magnétiques AP.
PCT/US2021/045132 2020-08-07 2021-08-07 Système et méthodes de traitement de cellules cancéreuses avec des champs magnétiques à polarité alternée WO2022035719A1 (fr)

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