WO2022026933A3 - Différenciation in vitro de cellules endocrines pancréatiques - Google Patents

Différenciation in vitro de cellules endocrines pancréatiques Download PDF

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Publication number
WO2022026933A3
WO2022026933A3 PCT/US2021/044081 US2021044081W WO2022026933A3 WO 2022026933 A3 WO2022026933 A3 WO 2022026933A3 US 2021044081 W US2021044081 W US 2021044081W WO 2022026933 A3 WO2022026933 A3 WO 2022026933A3
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WO
WIPO (PCT)
Prior art keywords
cells
pancreatic endocrine
endocrine cells
methods
vitro differentiation
Prior art date
Application number
PCT/US2021/044081
Other languages
English (en)
Other versions
WO2022026933A2 (fr
Inventor
George Harb
Chunhui XIE
Original Assignee
Vertex Pharmaceuticals Incorporated
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vertex Pharmaceuticals Incorporated filed Critical Vertex Pharmaceuticals Incorporated
Priority to CA3186968A priority Critical patent/CA3186968A1/fr
Priority to AU2021315814A priority patent/AU2021315814A1/en
Priority to US18/018,787 priority patent/US20240271100A1/en
Priority to EP21848634.8A priority patent/EP4189066A4/fr
Publication of WO2022026933A2 publication Critical patent/WO2022026933A2/fr
Publication of WO2022026933A3 publication Critical patent/WO2022026933A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0677—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

L'invention concerne des compositions et des méthodes se rapportant à la différenciation de cellules souches en cellules endocrines pancréatiques. Selon certains aspects, les méthodes de l'invention concernent la génération de cellules β pancréatiques, de cellules α, de cellules δ et de cellules EC in vitro. Selon certains aspects, l'invention concerne des compositions pharmaceutiques comprenant les cellules générées selon les méthodes décrites ici, ainsi que des méthodes de traitement et d'utilisation de celles-ci.
PCT/US2021/044081 2020-07-31 2021-07-30 Différenciation in vitro de cellules endocrines pancréatiques WO2022026933A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA3186968A CA3186968A1 (fr) 2020-07-31 2021-07-30 Differenciation in vitro de cellules endocrines pancreatiques
AU2021315814A AU2021315814A1 (en) 2020-07-31 2021-07-30 In vitro differentiation of pancreatic endocrine cells
US18/018,787 US20240271100A1 (en) 2020-07-31 2021-07-30 In vitro differentiation of pancreatic endocrine cells
EP21848634.8A EP4189066A4 (fr) 2020-07-31 2021-07-30 Différenciation in vitro de cellules endocrines pancréatiques

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063059563P 2020-07-31 2020-07-31
US63/059,563 2020-07-31

Publications (2)

Publication Number Publication Date
WO2022026933A2 WO2022026933A2 (fr) 2022-02-03
WO2022026933A3 true WO2022026933A3 (fr) 2022-04-07

Family

ID=80036824

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2021/044081 WO2022026933A2 (fr) 2020-07-31 2021-07-30 Différenciation in vitro de cellules endocrines pancréatiques

Country Status (5)

Country Link
US (1) US20240271100A1 (fr)
EP (1) EP4189066A4 (fr)
AU (1) AU2021315814A1 (fr)
CA (1) CA3186968A1 (fr)
WO (1) WO2022026933A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3833365A4 (fr) 2018-08-10 2022-05-11 Vertex Pharmaceuticals Incorporated Différenciation d'ilot dérivé de cellules souches
JP2023537327A (ja) 2020-07-31 2023-08-31 バーテックス ファーマシューティカルズ インコーポレイテッド 膵内分泌細胞の分化
IL311636A (en) 2021-11-01 2024-05-01 Vertex Pharma Differentiation of stem cell-derived pancreatic islets
CN115011544B (zh) * 2022-05-30 2023-11-17 广州国家实验室 体外诱导获得胰岛δ细胞的方法及其应用
WO2024020401A1 (fr) * 2022-07-19 2024-01-25 Lung Biotechnology Pbc Procédés de différenciation de cellules épithéliales ou basales

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150218522A1 (en) * 2013-06-11 2015-08-06 President And Fellows Of Harvard College Sc-beta cells and compositions and methods for generating the same
WO2020033879A1 (fr) * 2018-08-10 2020-02-13 Semma Therapeutics, Inc. Différenciation d'ilot dérivé de cellules souches

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030138951A1 (en) * 2001-10-18 2003-07-24 Li Yin Conversion of liver stem and progenitor cells to pancreatic functional cells
CA2460602A1 (fr) * 2004-03-05 2005-09-05 Raewyn Seaberg Cellules progenitrices multipotentes du pancreas
EP3024926B1 (fr) * 2013-07-26 2021-03-03 The J. David Gladstone Institutes, A Testamentary Trust Established under The Will of J. David Gladstone Génération de cellules de l'endoderme définitif et de cellules progénitrices pancréatiques
CA3092842A1 (fr) * 2018-03-02 2019-09-06 Vertex Pharmaceuticals Incorporated Procedes d'amelioration de la differenciation de cellules souches en cellules beta

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150218522A1 (en) * 2013-06-11 2015-08-06 President And Fellows Of Harvard College Sc-beta cells and compositions and methods for generating the same
WO2020033879A1 (fr) * 2018-08-10 2020-02-13 Semma Therapeutics, Inc. Différenciation d'ilot dérivé de cellules souches

Also Published As

Publication number Publication date
US20240271100A1 (en) 2024-08-15
EP4189066A2 (fr) 2023-06-07
WO2022026933A2 (fr) 2022-02-03
CA3186968A1 (fr) 2022-02-03
EP4189066A4 (fr) 2024-10-23
AU2021315814A1 (en) 2023-03-02

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