WO2022005142A1 - Dispositif de détection d'acide nucléique comprenant un ensemble de détection de lumière mobile - Google Patents

Dispositif de détection d'acide nucléique comprenant un ensemble de détection de lumière mobile Download PDF

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Publication number
WO2022005142A1
WO2022005142A1 PCT/KR2021/008124 KR2021008124W WO2022005142A1 WO 2022005142 A1 WO2022005142 A1 WO 2022005142A1 KR 2021008124 W KR2021008124 W KR 2021008124W WO 2022005142 A1 WO2022005142 A1 WO 2022005142A1
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WO
WIPO (PCT)
Prior art keywords
light
sample holder
sample
detection assembly
nucleic acid
Prior art date
Application number
PCT/KR2021/008124
Other languages
English (en)
Inventor
Jin Won Kim
Jin Seok Noh
Dong Woo Kang
Soon Joo Hwang
Seung Min Baik
Sang Min Kim
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Seegene, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Seegene, Inc. filed Critical Seegene, Inc.
Priority to KR1020227045143A priority Critical patent/KR20230015434A/ko
Publication of WO2022005142A1 publication Critical patent/WO2022005142A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G01N21/8483—Investigating reagent band

Definitions

  • the present disclosure relate to a nucleic acid device including a light detection assembly for detecting a light signal from a sample.
  • Nucleic acid amplification reaction well known as polynucleotide chain reaction (PCR) includes repeated cycles of doube-stranded DNA denaturation, annealing of the oligonucleotide primers to DNA templates, and extension/elongation of the primers with the DNA polymerase (Mullis et al., U.S. Patent Nos. 4,683,195, 4,683,202, and 4,800,159; Saiki et al., (1985) Science 230, 1350-1354). DNA denaturation is performed at about 95 °C, and anealing and primer elongation are performed at a lower temperature ranging frm 55 °C to 75 °C.
  • the light source emits excitation light to the sample, and the fluorescent material included in the sample excited by an excitation light emits fluorescence.
  • the fluorescent material included in the sample excited by an excitation light emits fluorescence.
  • the position of the light source is fixed, and when a hot lid that provides heat and pressure to a sample reaction vessel comes into contact with a sample holder, the light is irradiated from the light source, so the path of light may be longer depending on the distance between the light source and the hot lid. As the path of light increases, it is difficult to align the optical axis, and there is a problem in that optical interference occurs from the outside or optical loss occurs.
  • the hot lid moves to provide the pressure to the sample reaction vessel.
  • the hot lid may not be accurately positioned to correspond to each well of the sample reaction vessel.
  • the present inventors have tried to develop a light source that irradiates light with a stable light path while maintaining a stable excitation light path without distortion of the light path with respect to the sample holder, and optimize alignment between components receiving the light. As a result, the present inventors have found that the deviation due to distortion of the light path may be reduced by maintaining the stable excitation light path while accurately aligning the excitation light path according to the sample position.
  • the present disclosure may provide a nucleic acid detection device for the light signal detection, including a light detection assembly secured with mobility toward the sample.
  • a nucleic acid detection device comprising: a sample holder for accommodating a sample or a sample reaction vessel and being disposed in a sample holder assembly; a light detection assembly including a light source module comprising a light source for exciting the sample, and a detection module for detecting a signal emitted from the sample, and being disposed over the sample holder assembly; and a power for moving the light detection assembly up and down.
  • the nucleic acid detection device may further comprise a guide module for guiding the vertical movement of the light detection assembly when the light detection assembly moves up and down.
  • the guide module may comprise a plurality of shafts spaced apart from each other and arranged vertically parallel; and a connection member for connecting the light detection assembly to the plurality of shafts, respectively, and slidingly moving along the shafts.
  • the light detection assembly and the sample holder is positioned in an inside space defined by the plurality of shafts.
  • the power descends the light detection assembly such that light is incident toward the sample holder and light emitted from the sample holder is detected.
  • the power ascends the light detection assembly for access to the sample holder.
  • a hot lid providing heat and pressure to the sample reaction vessel or sample holder is positioned between the light detection assembly and the sample holder.
  • the hot lid is connected to a lower portion of the light detection assembly and moves simultaneously with the light detection assembly.
  • the hot lid moves independently of the movement of the light detection assembly.
  • the hot lid comprises a hole through which incident light and emission light pass.
  • the sample holder is horizontally movable, and a moving direction of the sample holder and a moving direction of the light detection assembly are perpendicular to each other.
  • the nucleic acid detection device may further comprise; an alignment pin positioned on a bottom surface of the light detection assembly to maintain alignment with the sample holder when the light detection assembly moves up and down; and an alignment hole positioned on a top surface of the sample holder assembly for accommodating the sample holder and inserted into an alignment pin.
  • the nucleic acid detection device may further comprise; an alignment hole positioned on a bottom surface of the light detection assembly; and an alignment pin positioned on a top surface of the sample holder assembly and inserted into the alignment hole.
  • the light detection assembly including the light source module and the detection module is movable up and down. Therefore, since the light path may be reduced compared to a device that irradiates the light from a fixed position, distortion of the light path until the light reaches the samples may be prevented, thereby securing the stable optical path, irradiating the light and moving the light source module and the detection module to a desired position in order to detect it.
  • the nucleic acid detection appratus includes the light source module for irradiating excitation light to the sample and the detection module for detecting emission light from the sample that is housed in in a box interior space that is shielded up, down, front, rear, left, and right, and a space, thereby blocking interference and light loss from the inside.
  • the nucleic acid detection device may guide the vertical movement of the light detection assembly within a predetermined region in order to prevent bias due to the load of the light detection assembly and change of alignment due to vertical movement, and stably provide the excitation light to the sample by assisting it.
  • FIG. 1 is a perspective view of a nucleic acid detection device for light signal detection including a movable light detection assembly according to an embodiment of the present disclosure.
  • FIG. 2 illustrates a light detection assembly including a light source module and a detection module according to an embodiment of the present disclosure.
  • FIG. 3 is a side view of a nucleic acid detection device according to an embodiment of the present disclosure showing a case in which the light detection assembly is descended.
  • FIG. 4 is a side view of the nucleic acid detection device according to an embodiment of the present disclosure showing a case in which the light detection assembly is ascended.
  • nucleic acid amplification reactions may be performed using a thermal cycler according to the present disclosure.
  • a nucleic acid amplification reaction may be performed by polymerase chain reaction (PCR), ligase chain reaction (LCR; see Wiedmann M, et al., “Ligase chain reaction (LCR)-overview and applications," PCR Methods and Applications 1994 Feb; 3(4):S51-64), gap filling LCR (GLCR; see WO 90/01069, European Patent No.
  • PCR polymerase chain reaction
  • LCR ligase chain reaction
  • GLCR gap filling LCR
  • Q-beta replicase amplification see Cahill P, et al., Clin Chem., 37(9):1482-5(1991), United States Patent No. 5,556,751
  • SDA strand displacement amplification
  • SDA nucleic acid sequence-based amplification
  • NASBA nucleic acid sequence-based amplification
  • TMA transcription-mediated amplification
  • TMA transcription-mediated amplification
  • RCA rolling circle amplification
  • RCA see Hutchison C.A., et al., Proc. Natl Acad. Sci. USA. 102:1733217336(2005)
  • the thermal cycler according to the present disclosure is useful for nucleic acid amplification reactions based on polymerase chain reactions.
  • a variety of nucleic acid amplification methods based on polymerase chain reactions have been known in the art.
  • such nucleic acid amplification methods include quantitative PCR, digital PCR, asymmetric PCR, reverse transcriptase PCR (RT-PCR), differential display PCR (DD-PCR), nested PCR, arbitrary priming PCR (AP-PCR), multiplex PCR, SNP genotyping PCR, and the like.
  • cycle refers to a single repeating unit.
  • a single cycle refers to a reaction including heat denaturation of a nucleic acid, hybridization or annealing of the nucleic acid with a primer, and primer extension.
  • a change in predetermined conditions is an increase in the number of repetitions, and the repeating unit in reactions, including a series of the above-described operations, is set to be a single cycle.
  • FIG. 1 is a perspective view of a nucleic acid detection device for light signal detection including a movable light detection assembly according to an embodiment of the present disclosure.
  • FIG. 2 illustrates a light detection assembly including a light source module and a detection module according to an embodiment of the present disclosure.
  • the nucleic acid detection device 100 is a thermal cycler.
  • the thermal cycler which is the nucleic acid detection device 100 detects an optical signal generated from a sample.
  • the optical signal may be luminescence, phosphorescence, chemiluminescence, fluorescence, polarized fluorescence, or other colored signal.
  • the optical signal generated from the sample may be, for example, a fluorescent signal.
  • the optical signal may be an optical signal generated in response to an optical stimulus applied to the sample.
  • the nucleic acid detection device 100 includes a light detection assembly 110, a sample holder 120, and a power 150.
  • the light detection assembly 110 includes a light source module 112 including a light source for excitation of a sample, and a detection module 118 for detecting a signal emitted from the sample.
  • the light detection assembly 110 is in the form of a box in which up, down, front, back, left and right are shielded so as to prevent interference due to light noise from the outside and to prevent internal light from leaking to the outside.
  • the light source module 112, the detection module 118, and a beam splitter 116 are positioned in an inside space of the box.
  • the shape of the light detection assembly 100 is not limited to the form of the box, and it is possible to be any form in which the up, the down, the front, the back, the left and the right are shielded so as to protect the interference from the outside and the light of the inside.
  • the left and right sides should be.
  • the light detection assembly 110 includes a light source module 111 including a plurality of light source units 112, a filter module 114, and a beam splitter 116, a detection module 118 including a plurality of detection units 121 and a hot lid 180.
  • the light source module 111 emits light to excite an optical label included in the sample.
  • the light source module 111 includes the plurality of light source units 112.
  • the light source module 111 includes the plurality of light source units 112a and 112b to irradiate excitation light to a plurality of sample regions of the sample holder 120 in which the sample is accommodated.
  • the plurality of light source units 112a and 112b may be light source units emitting light having the same wavelength characteristic. This means, for example, that the plurality of light source units 112a and 112b emit light of the same wavelength region, and the amount of light emitted for each wavelength region is the same.
  • the same herein may include not only exactly the same thing but also substantially the same thing. Substantially the same thing means that when light emitted from the two light source units 112a and 112b is irradiated to the same optical label through the same filter, the same type of emission light from the optical label is generated at the same level of light quantity.
  • the plurality of light source units 112a and 112b having substantially the same wavelength characteristics means that the deviation of the amount of light or the wavelength region of the plurality of light source units 112a and 112b may be within 20%, 15%, or 10%, but it is not limited thereto.
  • Each light source unit of the plurality of light source units 112a and 112b may include one or more light source elements.
  • the number of light source elements included in each light source unit may be, for example, one. In this case, one light source element may be one light source unit.
  • the light source unit may include two light source elements. In this case, two light source elements may be one light source unit.
  • the number of light source elements included in the light source unit is not limited to the above-mentioned embodiment.
  • the light source unit of the present disclosure may include 1000, 500, 100, 50, 40, 30, 20 or less light source elements.
  • Each light source unit of the plurality of light source units 112a and 112b is configured to irradiate light to different sample regions 123a and 123b, and each light source unit is assigned to one dedicated sample region.
  • each of the plurality of sample regions of the sample holder 120 is a region divided by irradiation region of an excitation light for each light source unit.
  • the light source module 111 may include the plurality of light source units including a first light source unit 112a and a second light source unit 112b. Alternatively, in the present disclosure, the light source module 111 may include a plurality of light source units including a first light source unit, a second light source unit, a third light source unit, and a fourth light source unit. Alternatively, in the present disclosure, the light source module 111 may include 10, 20, 30, 40, or 50 or less light source units.
  • the light source module 111 including a plurality of such light source units may include a light source unit support 117.
  • the plurality of light source units 112a and 112b may be disposed on the light source unit support 113.
  • one light source unit may be disposed on the light source unit support 113.
  • One or more light source units may be fixed to the light source unit support 117.
  • the shape of the light source unit support 113 may be circular, but it is not limited thereto, and it may have various shapes such as a circle, an ellipse, and a square.
  • the light source module 111 may include one light source unit support 117. Alternatively, the light source module 111 may include two light source unit supports. Alternatively, the light source module 111 may include four light source unit supports. Alternatively, the light source module 111 may include 10 or less light source unit supports 113.
  • the light emitted by the light source unit 112 may be referred to as excitation light.
  • the light emitted by the sample may be referred to as emission light.
  • the path of the excitation light emitted from the light source unit 112 may be referred to as an excitation path.
  • the path of the emission light emitted from the sample may be referred to as an emission path.
  • the light source unit 112 may include a light source element.
  • One light source unit may include one or more light source elements.
  • the light source may be a light emitting diode (LED), including an organic LED, inorganic LED, and quantum dot LED, or a laser unit including a tunable laser, a He-Ne laser, or a Ar laser.
  • the light source may be the LED.
  • the filter module 114 filters the light emitted from the light source unit 112 so that light of a specific wavelength region reaches the sample.
  • the filter module 114 includes a plurality of filter units.
  • the filter module 114 may include one or more filter units 115.
  • the filter module 114 may include two filter units 115a and 115b.
  • the filter module 114 may include four filter units.
  • Each of the filter units 115a and 115b includes a filter.
  • Each of the filter units 115a and 115b includes the filter that passes light in a wavelength region capable of exciting at least one of the optical labels.
  • the filter included in the filter unit may be a bandpass filter.
  • the bandpass filter refers to a filter that selectively transmits light in a predetermined wavelength region.
  • a wavelength region of light passing through the bandpass filter is referred to as a passband of the filter.
  • the passband may be donated in the form of a wavelength region.
  • a filter including a specific passband means a filter that passes light of a wavelength included in the specific passband.
  • the first filter unit 115a may be a first passband filter
  • the second filter unit 115b may be a second passband filter.
  • the first pass band and the second pass band may each include a wavelength region of light capable of exciting a specific optical label.
  • the optical label may be an optical label selected from the group consisting of FAM, CAL Fluor Red 610, HEX, Quasar 670, and Quasar 705.
  • the first filter unit 115a and the second filter unit 115b may pass light capable of exciting different optical labels.
  • the passbands of the first filter unit 115a and the second filter unit 115b may not overlap each other.
  • the filter units 115a and 115b included in the filter module 114 may be arranged to selectively excite different optical labels. Therefore, according to one embodiment of the present disclosure, the passband of each filter unit included in the filter module 114 may be different from each other.
  • the filter module 114 is disposed to be movable so that each of the filter units 115a and 115b may selectively filter the light emitted from the light source unit 112.
  • the filter module 114 may include a filter support 117.
  • the plurality of filter units 115a and 115b may be disposed on the filter support 117.
  • the filter units 115a and 115b may be fixed to the filter support 117.
  • the filter support 117 is disposed to be movable.
  • the filter units 115a and 115b fixed to the filter support 117 move by the movement of the filter support 117.
  • the shape of the filter support 117 is not particularly limited, and may have various shapes such as a circle, an ellipse, and a square.
  • the device 100 may include a moving unit (not shown) capable of moving the plurality of filter units 115a and 115b.
  • the filter support may be configured to be movable by a moving portion.
  • the moving portion may be, for example, a motor.
  • the motor may be, for example, an AC motor, a DC motor, a step motor, a servo motor, or a linear motor, preferably a step motor.
  • the moving portion may move the filter support 117 through, for example, a connecting shaft.
  • the movement may be, for example, a rotational movement that rotates about a connecting shaft.
  • a connection shaft for transmitting the power of the motor to the filter support may be configured to connect the motor and the filter support 117. Both ends of the connecting shaft may be directly connected to the filter support 117 and the motor to transmit power. Alternatively, one end of the connecting shaft may be connected to the filter support 117, and the other end may be indirectly connected to the motor through other power transmission means such as a gear, a belt, and a pulley.
  • the beam splitter 116 reflects and transmits the light incident from the light source unit 112. The light passing through the beam splitter 116 reaches the sample holder 120. The beam splitter 116 reflects and transmits light emitted from the sample. The beam splitter 116 may be configured so that light reflected by the beam splitter 116 may reach the detection module 180.
  • the detection module 118 detects the optical signal by generating an electrical signal according to the strength of the optical signal.
  • the detection module 118 may detect the fluorescence, which is an optical signal generated from the samples.
  • the detection module 118 may include a detection unit.
  • the detection unit may include a detector for detecting light.
  • the detection module 118 may detect the fluorescence emitted from the samples contained in the sample reaction vessels.
  • the fluorescent material is excited by the light source module 111, and the fluorescence is emitted from the fluorescent material.
  • the fluorescence may be received by the detection module 118 via the beam splitter 116.
  • the detection module 118 may detect the fluorescence by generating an electrical signal according to the intensity of the fluorescence.
  • the detection module 118 may be disposed at a distance to cover the fluorescence generated from the sample.
  • the detection module 118 may include a detection unit 121 and a detection filter unit 119.
  • the detection unit 121 may be a plurality of detection units.
  • Each detection unit of the plurality of detection units may include one or more detectors 122a and 122b.
  • the number of detectors included in the detection unit 121 may be, for example, one. In this case, one detector may be one detection unit. Alternatively, the detection unit 121 may include two detectors. In this case, two detectors may be one detection unit. However, the number of detectors included in the detection unit of the present disclosure is not limited to one embodiment.
  • the detection units of the present disclosure may include 1000, 500, 100, 50, 40, 30, 20 or fewer detectors.
  • Each detection unit of the plurality of detection units may be arranged to detect the emission light from different sample regions.
  • the detection filter unit 119 may be disposed in front of the detection unit 121.
  • the detection filter unit 119 may include a detection filter 125, and the detection filter 125 disposed in front of the detection unit 121 may be changed according to the wavelength of the emission light.
  • the detection filter 125 of the detection module 118 is a filter for selectively passing the emission light emitted from the optical label included in the sample. When the detector 122 detects light in a wavelength region other than the emission light emitted from the optical label included in the sample, the optical signal may not be accurately detected.
  • the detection filter 125 may allow the target to be accurately detected by selectively passing the emission light emitted from the optical label.
  • the detection unit 121 may include a detector 122.
  • the detector 122 is configured to detect the emission light emitted from the optical label included in the sample.
  • the detector 122 may detect the amount of light for each wavelength by dividing the wavelength of the light, or detect the total amount of light regardless of the wavelength.
  • the detector 122 may use, for example, a photodiode, a photodiode array, a photo multiplier tube (PMT), a CCD image sensor, a CMOS image sensor, an avalanche photodiode (APD), or the like.
  • the detector 122 is configured to detect the emission light emitted from the optical label included in the sample.
  • the detector 122 may be configured toward the sample holder 120 so that the emission light generated from the sample may directly reach the detector 122, or emitted through a reflector or an optical fiber. It may be directed towards the reflector or the optical fiber so that light may reach the detector.
  • the detector 122 may be configured toward the beam splitter 116 through which the emission light is reflected.
  • the detector 122 may be a plurality of detectors.
  • each of the plurality of detectors may be configured to detect the emission light generated in a predetermined region of the sample holder 120.
  • the first detector 122a is configured to detect the emission light emitted from the first sample region of the sample holder 120
  • the second detector 122b is configured to detect the emission light emitted from the second sample region of the sample holder 120.
  • the nucleic acid detecting device 100 may detect a plurality of signals in a first sample region of the sample holder 120, and also a second sample region of the sample holder 120.
  • the plurality of detectors may be configured in one detection module 118 to detect the emission light emitted from different sample regions, respectively.
  • the light detection assembly 110 may have a hexahedral shape including an top surface, a bottom surface, and front, rear, left, and right sides, and have an inside space through the top surface, bottom surface, and front and rear left and right sides.
  • the light source module 111 and the detection module 118 described above are accommodated and housed in the inside space. In FIG. 1, the light source module 111 and the detection module 118 are omitted for convenience of description.
  • the sample holder 120 is a component directly accommodating a sample or accommodating a reaction vessel including a sample.
  • the sample holder 100 positions the sample at a predetermined position, so that the optical stimulus from the light source module 111 arrives at the sample, and an optical signal generated from the sample arrives at the detection module 118.
  • the sample holder 120 may be mounted on the device when the device is operated, instead of being fixed to the device.
  • the sample holder 120 may accommodate a sample
  • a sample as represented herein may be used to comprehensively refer to a case in which the sample holder 120 directly accommodates a sample or a case in which the sample holder 120 accommodates a reaction vessel including a sample.
  • the heat-generating element may supply heat to the sample holder 120, so that the heat is transferred to the sample directly accommodated in the sample holder 120 or the sample accommodated in the reaction vessel.
  • the reaction vessel may be made of a variety of materials, such as plastic, ceramic, glass, or metal.
  • the sample holder 120 accommodating the reaction vessels may have the shape of a block or a plate.
  • the sample holder 120 accommodating the reaction vessels may include recesses (e.g. wells) to accommodate the reaction vessels or may have a flat surface.
  • the sample holder 120 accommodating the reaction vessels may have a structure by which the positions of the reaction vessels may be guided or the reaction vessels may be fixed.
  • a single sample holder 120 is fabricated such that the single sample holder 120 may accommodate one or more samples.
  • a typical example of the sample holder 120 accommodating the reaction vessels is a thermal block.
  • the thermal block may include a plurality of wells or holes respectively allowing a reaction vessel to be accommodated therein.
  • the sample holder 120 accommodating the reaction vessels may refer to a state in which the reaction vessels are disposed in the plurality of wells of the sample holder 120 or are disposed on assigned positions of the sample holder.
  • the reaction vessels are respectively used to accommodate a sample to be analyzed.
  • Examples of the reaction vessel include a variety of shapes, e.g. a tube, a vial, a strip to which a plurality of single tubes are connected, a plate to which a plurality of tubes are connected, a microcard, a chip, a cuvette, or a cartridge.
  • the sample holder 120 directly accommodating the sample may have the shape of the above-described reaction vessel.
  • the sample holder 120 directly accommodating the sample may be made of the material of the above-described reaction vessel.
  • the sample holder 120 is made of a material having thermal conductivity. When the sample holder 120 is in direct contact with the sample or in contact with the reaction vessels, heat may be transferred from the sample holder 120 to the sample or the sample in the reaction vessel.
  • the sample holder 120 may be made of metal, such as aluminum, gold, silver, nickel, or copper, or made of plastic or ceramic.
  • the sample holder 120 is configured to accommodate the plurality of samples, and a reaction for detection such as the nucleic acid amplification reaction may occur by controlling the temperature of the plurality of samples.
  • a reaction for detection such as the nucleic acid amplification reaction may occur by controlling the temperature of the plurality of samples.
  • the sample holder 120 is a heat block in which a plurality of wells are configured
  • the sample holder 120 is configured as a single heat block, and all wells of the heat block are configured not to be thermally independent from each other.
  • the temperatures of all wells in which the samples are accommodated in the sample holder 120 are the same, and the temperatures of the received samples may not be adjusted according to different protocols.
  • the sample holder 120 may be configured to adjust the temperature of some of the samples accommodated in the sample holder 120 according to different protocols.
  • the sample holder 120 may include two or more thermally independent reaction regions. Each reaction region is thermally independent. No heat is transferred from one reaction region to another. For example, there may be an insulating material or an air gap between the reaction regions.
  • the temperature of each of the reaction regions may be independently controlled.
  • a reaction protocol including temperature and time may be individually set for each of the reaction regions, and each of the reaction regions may perform a reaction according to an independent protocol. Since the reaction proceeds according to an independent protocol in the reaction regions, the time points of light detection in the reaction regions are independent of each other.
  • the sample holder 120 includes a plurality of sample regions.
  • the sample region is a region divided by the irradiation region of the excitation light of the light source unit.
  • the sample holder 120 may include a plurality of sample regions.
  • Each of the plurality of sample regions of the present disclosure may refer to a region on the sample holder 120 in which the samples to which an optical signal detection reaction is performed by the light source unit 112 are located.
  • the sample region of the present disclosure may refer to a group of reaction sites in which the optical signal detection reaction is performed by the same light source unit among a plurality of reaction sites included in the sample holder 120. That is, the sample region is a region divided by the irradiation region of the excitation light of the light source unit 112.
  • the sample holder 120 positions the sample in a predetermined position, such that the optical stimulus arrives at the sample and the optical signal generated from the sample arrives at the detection module 118.
  • the sample holder 120 may perform a process for detecting an optical signal from the sample, such as temperature control of the sample, if necessary.
  • each sample region is not defined over two or more reaction regions, but is included in one reaction region or may be defined over the same as one reaction region.
  • the optical signal detection may be performed by a light source unit and a filter unit different from each other in the two or more thermally independent reaction regions at which light detection time points are independent from each other.
  • the sample holder 120 may include two or more thermally independent reaction regions, and each of the sample regions may be defined to be included in any one of the two or more thermally independent reaction regions.
  • the sample holder 120 is not divided into a plurality of sample regions, but in the present disclosure, the sample holder 120 is divided by the the irradiation region of the excitation light for each light source unit of the plurality of light source units 112.
  • hollow spaces may be provided between the wells to reduce heat capacity.
  • the plurality of wells of the sample holder 120 are regularly arranged.
  • the plurality of wells are arranged in a matrix consisting of columns and rows.
  • the plurality of wells may be provided as, for example, 16 wells having a 4x4 array, 24 wells having a 6x4 array, 32 wells having a 4x8 array, 60 wells having a 5x12 array, 90 wells having a 5x18 array, and 96 wells having an 8x12 array.
  • the 16 wells, the 32 wells, and the 96 wells may generally be used, although the present disclosure is not limited thereto.
  • the shape, size, or the like of the wells may be determined to be adequate to the reaction vessels accommodated therein.
  • the number of the wells of the sample holder 120 is equal to or less than 500, 400, 300, 200, 100, or 50.
  • the number of the wells of the sample holder 120 is equal to or more than 4, 8, 10, 20, 30, or 40.
  • the sample holder 120 may be accommodated in the sample holder assembly 160 and disposed thereon.
  • the sample holder assembly 160 disposes the sample holder 120 thereon, and exposes all or part the sample holder 120 to the outside to accommodate a sample or to facilitate detachment of the sample reaction vessel.
  • the upper portion of the sample holder assembly 160 is a region in which all or part of the sample holder 120 is exposed, and may refer to a region that may face the lower portion of the light detection assembly 110 upon contact with the hot lid 180 and the sample holder 120 providing heat and pressure to the sample reaction vessel.
  • a heat generating element (not shown) for controlling the temperature of the sample holder 120 beneath the sample holder 120, and a heat sink (not shown) disposed thereunder are further included. While the upper portion of the sample holder 120 may be exposed to the outside, the heat generating element and the heat sink positioned below the sample holder 120 are housed inside the case of the sample holder assembly 160.
  • the case refers to a structure surrounding the outside of the sample holder assembly 160.
  • the sample holder assembly 160 may further include a heat cooling fan for dissipating the heat of the heat cooling plate.
  • the heat cooling fan may be located below or on the side of the cooling plate, and according to various embodiments, the cooling fan may be disposed inside or outside the sample holder assembly 160.
  • the sample holder assembly 160 is horizontally movable to the outside of the nucleic acid detection device 100 through an LM guide that provides a horizontal movement path on the bottom surface 20 of the nucleic acid detection device 100.
  • the user since the sample holder assembly 160 is drawn into and out of the nucleic acid detection device 100 like a drawer, the user removes the sample or the sample reaction vessel from the sample holder 120 or holds the sample or the sample reaction vessel in the sample holder 120 to allow access to the sample holder 120 in order to prepare the sample for thermal cycling.
  • the horizontal movement of the sample holder assembly 160 in the same manner as the drawer is possible by receiving power from a motor under the control of a processor (not shown), and the motor may be, for example, a stepper motor.
  • the moving direction of the sample holder assembly 160 and the moving direction of the light detecting assembly 110 are perpendicular or substantially perpendicular to each other.
  • the power 150 moves the light detection assembly 110 up and down in the inside space.
  • the power 150 receives power and operates under the control of a processor (not shown).
  • the power 150 may be any suitable motor known in the art with linear or rotary motion, such as a DC motor, an AC motor, or a step motor.
  • the motor 150 is an electric motor that rotates by a predetermined angle by a pulse-type input voltage, and continuously rotates by a series of input pulse trains.
  • the motor may include a stator and a rotation shaft that is relatively rotated by the stator.
  • the motor 150 may move the light detection assembly 110 up and down by, for example, driving the rotation shaft.
  • the rotational motion of the rotation shaft is converted into a linear reciprocating motion so that the light detection assembly is movable in the vertical direction in the inside space of the nucleic acid detection device 100.
  • the member 155 may be positioned between the light detection assembly 110 and the power 150.
  • One side of the member 155 is connected to the power 150 and the other side to the light detection assembly 110, respectively, to transmit a driving force generated from the power 150 to the light detection assembly 110 connected to the other side of the light detection assembly 110 so that the light detection assembly is movable in the vertical direction in the inside space of the nucleic acid detection device 100.
  • the power 150 contributes to substantially moving the light detection assembly 110 up and down inside the nucleic acid detection device 100 by moving the light detection assembly 110 up and down through the member 155.
  • the power 150 may be positioned on the inner top surface 10 in the inside space.
  • the member 155 connecting the power 150 and the light detection assembly 110 is extended to is connected from an upper portion of the inner top surface 10 to a lower portion of the inner top surface 10 through the through hole 5 of the upper portion.
  • the power 150 positioned on the inner top surface 10 is connected to the light detection assembly 110 positioned below the inner top surface 10 through a member 155 to move the light detection assembly 110 up and down.
  • the nucleic acid detection device 100 of the present disclosure is shown in a structure in which the light detection assembly 110 is movably supported up and down or vertically with respect to the sample holder 120, but the light detection assembly 110 is not limited up and down or vertically in the direction of movement. According to various embodiments, the light detection assembly 110 may move front/rear, left/right, or horizontally with respect to the sample holder 120 , and may move front/rear, left/right or horizontally of the light detection assembly 110. To enable this, the structure of the nucleic acid detection device 100 of the present disclosure may be different from that of FIG. 1.
  • the light detection assembly 100 is disposed over the sample holder 120.
  • the light detection assembly 110 may ascend from the sample holder 120 or descend toward the sample holder 120 while moving up and down according to the driving of the motor 150.
  • the nucleic acid detection device 100 of the present disclosure may further include a guide module 130 for guiding the vertical movement of the light detection assembly 110 when the light detection assembly 110 is moved up and down.
  • the guide module 130 includes a plurality of shafts 132, 134, 136, 138 and a connecting member 133.
  • the plurality of shafts 132, 134, 136 and 138 are spaced apart from each other and vertically parallel to each other.
  • the connecting member 133 connects the light detection assembly 110 to the plurality of shafts 132, 134, 136 and 138, respectively, and moves along the shaft in a sliding manner.
  • the shaft according to an embodiment of the present disclosure substantially defines the longitudinal direction of the nucleic acid detecting device 100 according to the present disclosure, and may be plural.
  • the plurality of shafts 132, 134, 136, 138 provide a movement path of the light detection assembly 110.
  • the plurality of shafts 132, 134, 136 and 138 may have a linear shape elongated in the longitudinal direction, and may be configured to extend from a lower portion to an upper portion of the nucleic acid detection device 100.
  • FIG. 1 four shafts are illustrated as an example, but the present disclosure is provided in more detail, and the shaft of the present disclosure is not limited thereto.
  • each shaft 132, 134, 136 and 138 has both ends fixed to the inner top surface 10 and the inner bottom surface 20 of the nucleic acid detection device 100, respectively, but the scope of the present disclosure is not limited thereto. According to an embodiment, it may be fixedly disposed between the inner top surface 10 and the inner bottom surface 20 by a separate means.
  • the inner top surface 10 and the inner bottom surface 20 are configured inside the housing or case of the nucleic acid detection device 100, and the inner top surface 10 fix the ascending position of the light detection assembly 110. Accordingly, when the light detection assembly 110 comes into contact with the inner top surface 100, it does not move any more.
  • the inner bottom surface 20 supports the sample holder assembly 160 from the lower.
  • the plurality of shafts 132, 134, 136 and 138 are spaced apart from each other to form an inside space 30 defined by a structure arranged in parallel to each other, and the sample holder assembly 130 including the light detection assembly 110 and the sample holder 120 is positioned in the inside space 30.
  • the connecting member 133 may include a slide member 131 that moves along the shaft in a sliding manner, and a moving block 135 that is coupled to one side of the slide member 131 to connect the light detection assembly 110 to the slide member 131.
  • the slide member 131 may reciprocate in a straight line along the linear axis of each shaft 132, 134, 136 and 138.
  • the moving block 135 is coupled to the outside of the slide member 131, and is coupled to and installed on the slide member 131 so that the light detection assembly 110 may move in a sliding manner.
  • the plurality of shafts 132, 134, 136 and 138 provide a movement path for the light detection assembly 110 to move up and down.
  • the plurality of shafts 132, 134, 136 and 138 is also a means for supporting vertical alignment on the movement path of the light detection assembly 110.
  • the light detection assembly 110 performs an alignment operation by moving relative to the sample holder 120 along the plurality of shafts 132, 134, 136 and 138 of the guide module 130.
  • the nucleic acid detection device 100 of the present disclosure includes a chassis 24 on the front and rear, left and right, or front and rear left and right sides of the light detection assembly 110. Since the chassis 24 supports the vertical movement axis of the light detection assembly 110 together with the plurality of shafts 132, 134, 136 and 138, and at the same time, supports the upper portion 10 and the lower portion 20 of the nucleic acid detection device 100, it is possible to prevent the inclination of the light detection assembly 110.
  • the light detection assembly 110 may more precisely align the light path of the light source toward the sample holder 120, and avoid the risk of crosstalk.
  • the sample holder assembly 160 on which the sample holder 120 is disposed horizontally moves in and out of the nucleic acid detection device 100, it is necessary to control the vertical movement of the light detection assembly 110 in consideration of displacement due to external movement from the internal fixed position of the sample holder 120. For example, when the sample holder assembly 160 deviates from the fixed position, the movement of the light detection assembly 10 descending toward the sample holder 120 may be stopped to irradiate light through the controller.
  • the nucleic acid detection device 100 of the present disclosure may further include a plate-shaped vertical support 135 that auxiliaryly supports the vertical alignment of the shafts 132, 134, 136 and 138.
  • the vertical support 135 may have the same height as the shafts 132, 134, 136 and 138, and have a vertical front plate and a vertical rear plate shape in a spaced relationship in front and rear, or inner left and right directions inside the nucleic acid detection device 100. Accordingly, the light detection assembly 110 and the sample holder assembly 160 are positioned in the inside space 30 of the plurality of shafts 132, 134, 136 and 138, and the light detection assembly 110 and a sample holder assembly 160 are located between the vertical front plate and the vertical rear plate.
  • the vertical support is described in the form of a plate, the shape of the vertical support is not limited thereto, and may have various shapes such as a circle and a square.
  • the light detection assembly 110 moves up and down in the inside space 30 of the nucleic acid detection device 100 through the guide module 130 based on the power of the motor 150 while emiting light to excite the optical label included in the sample of the sample holder 120, and detecting the light emitted from the sample.
  • the power 150 descends the light detection assembly 110 so that light is incident toward the sample holder 120 and the light emitted from the sample holder 120 is detected.
  • FIG. 3 is a side view of a nucleic acid detection device according to an embodiment of the present disclosure showing a case in which the light detection assembly is descended.
  • the light detection assembly 110 may be descended so that light is incident on the sample holder 120 and the light emitted from the sample holder 120 is detected.
  • the light detection assembly 110 includes the light source module 111 for excitation of the sample, it is positioned at a movable position toward the sample holder 120 so as to irradiate light to the sample, and the movement of the light detection assembly 110 is controlled through the power 150 so that the light detection assembly 110 may be located at a predetermined position where light may be irradiated to the light.
  • the predetermined position at which the light detection assembly 110 may irradiate light to the sample is a state in which the light detection assembly 110 is in contact with the sample holder 120, as shown in FIG. 3.
  • An operation on the degree of contact of the assembly 110 to the sample holder 120, that is, the predetermined position to which light may be irradiated may be performed on a computer system programmed to precisely control the movement position when the light detection assembly 110 in the nucleic acid detection device 100 of the present disclosur moves up and down.
  • the programmed computer system may be substantially referred to as a controller (not shown) of the nucleic acid detection device 100 of the present disclosure.
  • the controller may be used to control functions of the nucleic acid detection device 100 of the present disclosure.
  • the controller sets the ascending and descending positions of the light detection assembly 110 in the nucleic acid detection device 100 in advance, and when the light detection assembly 110 arrives at a set specific position, the vertical movement of the light detection assembly 110 may be controlled in such a manner as to stop or maintain the driving of the power 150.
  • the controller may control not only the ascending and descending positions of the light detection assembly 110 but also the light detection assembly 110 to move to only a specific distance. For example, when the rotation speed of the motor of the power 150 is measured and a specific rotation speed is reached, the rotation of the motor may be stopped. In this case, since the moving distance may be controlled according to the number of motor rotations in the ascending (or forward) or descending (or reverse) direction, the light detection assembly 110 moves in either direction by temporarily pausing when moving up and down. Since the movement of the light detection assembly 110 in progress is stopped and movement in the opposite direction is immediately possible, the mobility and direction changeability of the light detection assembly 110 are improved.
  • the nucleic acid detection device 100 of the present disclosure may use a proximity sensor as an example.
  • the proximity sensor senses an object by emitting an electromagnetic field or a beam, and the proximity sensor may be used to sense the position of the light detection assembly 110. For example, when the light detection assembly 110 is in a designated position, the proximity sensor senses it and sends feedback to the controller, thereby contributing to the controller to controll the ascending or descending of the light detection assembly 110.
  • sensors such as an inductive sensor or a photo sensor
  • the present disclosure may selectively adopt various types of sensors, and the present disclosure does not limit the types of sensors.
  • FIG. 4 is a side view of the nucleic acid detection device according to an embodiment of the present disclosure showing a case in which the light detection assembly is ascending from the sample holder 120.
  • the power 150 ascends the light detection assembly 110.
  • the nucleic acid detection device 100 of the present disclosure requires user access for loading the sample into the sample holder 120 or for detaching the sample reaction vessel. For this, the sample holder 120 should be withdrawn to the outside of the nucleic acid detection device 100. At this time, when the light detection assembly 110 is located at a position for light incident on the sample holder 120, it is not easy to take out the sample holder 120, so the motor 150 moves the light detection assembly 110 upwardly away from the sample holder 120 to prevent unnecessary friction or contact when the sample holder 120 is withdrawn to the outside.
  • the position of the light detection assembly 110 may be sensed via the proximity sensor. For example, when the light detection assembly 110 moves in the forward direction (ascending direction) to reach a specific position (ascended point), the proximity sensor sends feedback to the controller so that the controller controls the ascending of the light detection assembly 110.
  • a hot lid 180 is positioned between the light detection assembly 110 and the sample holder 120.
  • the hot lid 180 provides heat and pressure to the sample reaction vessel or sample holder 120.
  • the temperature and position of the hot lid 180 are controlled.
  • the nucleic acid detection device 100 raises and maintains the temperature of the hot lid 180, and brings the hot lid 180 into contact with the sample holder 120 or the sample reaction vessel.
  • the nucleic acid detection device 100 heats or cools the sample holder 120 when the hot lid 180 contacts the sample holder 120 to perform the amplification reaction of the samples accommodated in the form the sample holder 120 or the sample reaction vessels 120.
  • the hot lid 180 includes an upper plate 182 and a hot plate 184.
  • the hot lid 180 is positioned on the sample holder 120 and provides pressure to the sample holder 120.
  • the hot lid 180 moves downward under pressure, and the hot lid 180 contacts the sample holder 120 to apply pressure to the sample holder 120 or the sample reaction vessels.
  • the hot plate 184 of the hot lid 180 may have a length or size sufficient to cover the sample holder 120.
  • the upper plate 182 is a portion that receives the pressure
  • the hot plate 184 is a portion that is in contact with or spaced apart from the sample reaction vessels and maintains a high temperature.
  • An elastic member 186 is positioned between the upper plate 182 and the hot plate 184. When the hot plate 134 comes into contact with the sample reaction vessel and the upper plate 182 descends, the elastic member 186 is compressed and pressure is applied to the hot plate 184.
  • the elastic member 186 may be a spring, a rubber, or the like.
  • a packing member 188 may be configured under the heat plate 184 of the hot lid 180 in order to maximize the heat insulation and cooling effect of the hot lid 180.
  • the packing member 188 may be made of rubber. However, the present disclosure is not limited thereto, and various materials may be used as the packing member 188.
  • the packing member 188 may close the gap when in contact with the sample holder 120 to block external heat from flowing into the sample holder 120 and the internal heat from flowing out to the outside to maximize the insulating and the cooling effect.
  • the packing member 188 configured on the hot lid of the present disclosure may be configured to protrude under the hot plate 184 with an appropriate length while maintaining the same width as the width of the hot plate 184.
  • the left and right sides of the packing member 188 are configured to have an inclined surface of an appropriate inclination toward the sample holder 120, so that the hot lid 180 moves downward under pressure and comes into contact with the sample holder 120 to more easily pressure the sample reaction vessels of the sample holder 120.
  • the packing member 188 may have a length or size sufficient to cover the sample holder 120.
  • the width and/or area of the packing member 188 may be longer or larger than the width and/or area of the sample holder 120.
  • the hot lid 180 is connected to the lower portion of the light detection assembly 110 and may move simultaneously with the vertical movement of the light detection assembly 110. Accordingly, the hot lid 180 moves in a direction in which the light detection assembly 110 is contacted with or spaced apart from the sample holder 120 through vertical movement, and at the same time, the hot lid 180 under the light detection assembly 110 is directly contacted with or spaced apart from the sample holder 120.
  • the hot lid 180 may be disposed extending from the inside space 30 of the light detection assembly 110 to the outside.
  • the upper portion of the hot lid 180 may be fixed to the inside space 30 of the light detection assembly 110, and the lower portion may be exposed to the outside of the light detection assembly 110 to contact the sample holder 120.
  • the hot lid 180 may move independently of the movement of the light detection assembly 110.
  • the hot lid 180 and the light detection assembly 110 exist as independent components, and the hot lid 180 may be separately disposed at a position adjacent to the light detection assembly 110 by other fixing means.
  • Movement of the hot lid 180 precedes or follows the light detection assembly 110 via the light detection mechanism of the present disclosure. For example, when light is emitted to excite the optical label included in the sample, the hot lid 180 moves to contact the sample holder 120 prior to the movement of the light detection assembly 110, and when the light detection assembly 110 is ascended, the light detection assembly 110 reaches the ascended position, or the hot lid 180 moves from the sample holder 120 at the same time or during the ascending.
  • the hot lid 180 includes a hole through which the incident light and the emission light may pass.
  • the hot lid 180 includes a plurality of holes.
  • the holes of the hot lid 180 are configured at positions corresponding to the holes of the sample holder 120.
  • the light irradiated from the above-described light source unit and transmitted through the beam splitter 116 passes through the hole of the hot lid 180 and reaches the sample holder 120.
  • the nucleic acid detection device 100 may further include an alignment pin 26 positioned at the lower part of the light detection assembly 110 in order to maintain the alignment with the sample holder 120 when the light detection assembly 110 moves up and down, and an alignment hole 22 positioned on the upper portion of the sample holder assembly 160 for accommodating the sample holder 120 and into which the alignment pin 26 is inserted.
  • the nucleic acid detection device 100 may further include an alignment hole 22 located at the lower portion of the light detection assembly 110 and an alignment pin 26 located on the upper portion of the sample holder assembly 160 and into which the alignment hole 22 is inserted.
  • the alignment pins 26 may be provided on either the lower portion of the light detection assembly 110 or the upper portion of the sample holder assembly 160, respectively, and are disposed on the other one corresponding to the alignment pin 26.
  • the alignment hole 22 into which the alignment pin 26 is inserted may be provided.
  • the alignment pin 26 and the alignment hole 22 corresponding thereto may be provided in order to maintain the alignment of the light detection assembly 110 and the sample holder 120, or to more precisely guide the movement when the light detection assembly 110 descends toward the sample holder 120.
  • the alignment herein refers the optimal arrangement to form an optical path, which is a range through which light passes, so that light irradiated from the light detection assembly 110 passes through each well of the hot lid 180 to reach each sample of the sample holder 120.
  • the upper portion of the sample holder assembly 160 is a region in which all or part of the sample holder 120 is exposed, and the light detection assembly 110 may refer to a region that may face the lower part of the sample holder 120 when the hot lid 180 and the sample holder 120 come into contact with each other.
  • the alignment pin 26 and the alignment hole 22 exist to be paired with each other, and the alignment pin 26 and the alignment hole 22 may consist of two or more.
  • the alignment hole 22 and the alignment pin 26 may prevent tilt that may occur due to a load or a vertical movement of the light detection assembly 110.
  • the alignment pin 26 may be used by selecting any one of a circle, a square, a pentagon, and a hexagon in cross section in a column shape, and the alignment hole 22 is determined according to the shape of the alignment pin 26.
  • the alignment pin 26 is separated from the alignment hole 22 when the light detection assembly 110 is ascended, and is coupled to the alignment hole 22 when the light detection assembly 110 is descended.

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Abstract

Dans un dispositif selon la présente invention, un ensemble de détection de lumière comprenant un module de source de lumière et un module de détection est mobile vers un porte-échantillon. Comme l'ensemble de détection de lumière verticalement mobile peut être aligné avec précision vers le porte-échantillon, la lumière peut être fournie avec précision sans distorsion du trajet de lumière d'excitation irradié vers l'échantillon.
PCT/KR2021/008124 2020-06-29 2021-06-28 Dispositif de détection d'acide nucléique comprenant un ensemble de détection de lumière mobile WO2022005142A1 (fr)

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Citations (5)

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Publication number Priority date Publication date Assignee Title
WO2007049843A1 (fr) * 2005-10-28 2007-05-03 Goodgene Inc. Scanner de biopuces
JP2009074934A (ja) * 2007-09-20 2009-04-09 Miura Sensor Laboratory Inc 試料分析装置
WO2009054647A2 (fr) * 2007-10-25 2009-04-30 Seed Biochips Co., Ltd. Appareil d'analyse portable à base de pcr
KR101214479B1 (ko) * 2009-07-02 2012-12-27 이재수 화학발광 또는 형광 이미지 획득장치
US20190136297A1 (en) * 2003-05-08 2019-05-09 Bio-Rad Laboratories, Inc. Systems and methods for fluorescence detection with a movable detection module

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190136297A1 (en) * 2003-05-08 2019-05-09 Bio-Rad Laboratories, Inc. Systems and methods for fluorescence detection with a movable detection module
WO2007049843A1 (fr) * 2005-10-28 2007-05-03 Goodgene Inc. Scanner de biopuces
JP2009074934A (ja) * 2007-09-20 2009-04-09 Miura Sensor Laboratory Inc 試料分析装置
WO2009054647A2 (fr) * 2007-10-25 2009-04-30 Seed Biochips Co., Ltd. Appareil d'analyse portable à base de pcr
KR101214479B1 (ko) * 2009-07-02 2012-12-27 이재수 화학발광 또는 형광 이미지 획득장치

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