WO2021261647A1 - Virus detecting device using dielectric spectroscopy and detecting method therefor - Google Patents

Abstract

The present invention relates to a virus detecting device using dielectric spectroscopy and a detecting method therefor and, more specifically, to a device for detecting specific viruses by measuring complex permittivity spectra and a detecting method therefor. The virus detecting device and the detecting method according to the present invention can detect specific viruses quickly and accurately from even a small amount of samples by measuring complex permittivity spectra of viruses through electrochemical methods.

Description

Virus detection apparatus using genome spectroscopy and detection method therefor

The present invention relates to an apparatus for detecting a virus using genome spectroscopy and a method for detecting the same, and more particularly, to an apparatus and method for detecting a specific virus by measuring the complex dielectric constant spectra of the virus.

Viruses are infectious agents that are smaller than bacteria. It is composed of the genetic material RNA or DNA and the protein surrounding the genetic material. Viruses can be classified into plant viruses, animal viruses, and bacterial viruses (phages) according to the type of host. However, in most cases, according to the type of nucleic acid, it is divided into DNA virus subfamily and RNA virus subfamily, which are further subdivided into classes, orders, and families.

Viruses are divided into three types of high pathogenicity, low pathogenicity, and non-pathogenicity according to their pathogenicity. is being studied extensively.

These highly pathogenic viruses may include not only infectious viruses such as avian influenza or foot-and-mouth disease that cause disease in animals, but also infectious viruses such as corona, MERS, SARS or swine flu that cause acute and serious diseases in the human body. Since these infectious viruses spread to a large number of individuals within a short period of time due to population densification and an increase in group breeding, causing a lot of damage, it is desirable to prevent further spread through a prompt response as possible when these viruses appear.

Various studies are being conducted to detect the onset of these highly pathogenic viruses at an early stage. Specifically, there are many cases of checking whether an individual is infected using a kit, etc., but in this case, there is a problem in that it is difficult to simultaneously detect a large area by directly collecting a sample of blood or the like from the individual to be detected.

Furthermore, when collecting from such an individual, the individual is infected with the disease and can be detected after the onset in most cases.

Currently, the diagnosis of these highly pathogenic viruses is performed by virus isolation and serological tests. Among these, serological tests include hemagglutination inhibitory reaction (HI), agar sedimentation reaction (AGP), and enzyme immunoassay (ELISA).

Among them, the ELISA (Enzyme Immunoassay) method has the disadvantage of requiring laboratory equipment such as a spectrophotometer. Although it is very sensitive, it has the disadvantage of low specificity.

The present inventor completed the present invention by confirming that a specific virus can be detected by measuring the complex permittivity spectra of the genome while studying genome spectroscopy for virus detection.

Dielectric spectroscopy is a type of impedance method and measures the dielectric properties of an electrolyte over a high frequency region of several tens of GHz. Through this, dielectric spectroscopy can provide information on the types and concentrations of various polar chemical species present in the electrolyte, ie, free solvents and ion pairs having a dipole moment.

In order to solve the above problems, the present invention aims to provide an apparatus and a method for detecting a specific virus quickly and accurately by measuring the complex permittivity spectra of the virus through an electrochemical method even with a small amount of sample, and a method for detecting the same do.

In order to achieve the above object, a virus detection apparatus according to the present invention includes a sample unit 100 for injecting a sample to be measured; a probe unit 200 for irradiating a broadband electromagnetic wave to the sample; a measuring unit 300 for measuring the complex permittivity of the sample with respect to the irradiated broadband electromagnetic wave; and a detection unit 400 for detecting a virus from the complex dielectric constant.

Here, the probe unit 200 may include an open coaxial line probe.

Here, the frequency of the broadband electromagnetic wave may be within the range of 0.1 to 50 GHz.

Here, the measurement unit 300 may include a vector network analyzer.

Here, the measurement unit 300 may measure a real part value, an imaginary part value, and a dielectric relaxation frequency with respect to the complex dielectric constant of the sample.

Here, the detection unit 400 may detect the virus in the sample through a real part, an imaginary part, and a dielectric relaxation frequency for the complex dielectric constant of the sample.

Here, the display unit for displaying the detected virus on the display screen; may further include.

A virus detection method according to the present invention includes a first step of injecting a sample to be measured; a second step of irradiating a broadband electromagnetic wave to the sample; a third step of measuring the complex permittivity of the sample with respect to the irradiated broadband electromagnetic wave; and a fourth step of detecting the virus from the complex dielectric constant.

Here, the frequency of the broadband electromagnetic wave may be within the range of 0.1 to 50 GHz.

Here, a fifth step of displaying the detected virus on a display screen may be further included.

The virus detection apparatus and the detection method of the present invention according to the above-described method can quickly and accurately detect a specific virus by measuring the complex dielectric constant spectra of the virus through an electrochemical method even with a small amount of sample.

1 schematically shows each configuration of a virus detection apparatus according to an embodiment of the present invention.

2 is a view showing an overall configuration in which each configuration of the virus detection apparatus according to an embodiment of the present invention is organically combined.

3 is a photograph showing the actual state of the virus detection apparatus of the present invention including an open coaxial probe corresponding to the probe unit of the present invention and a vector network analyzer corresponding to the measuring unit of the present invention.

4 is a flowchart illustrating a virus detection method according to an embodiment of the present invention for each step.

5 is a diagram illustrating an experiment for measuring the complex permittivity of a sample in an aqueous solution state by using the virus detection apparatus according to an embodiment of the present invention.

6 is a graph showing the scattering parameter response characteristics of a sample in an aqueous solution state using the virus detection apparatus according to an embodiment of the present invention.

7 is a graph showing the complex dielectric constant spectra of a sample in an aqueous solution state by using the virus detection apparatus according to an embodiment of the present invention.

A virus detection apparatus according to the present invention includes a sample unit 100 for injecting a sample to be measured; a probe unit 200 for irradiating a broadband electromagnetic wave to the sample; a measuring unit 300 for measuring the complex permittivity of the sample with respect to the irradiated broadband electromagnetic wave; and a detection unit 400 for detecting a virus from the complex dielectric constant.

The terms used in this application are only used to describe specific examples. Therefore, for example, a singular expression includes a plural expression unless the context clearly requires it to be singular. In addition, terms such as “include” or “include” as used in the present application are used to clearly indicate that the features, steps, functions, components, or combinations thereof described in the specification exist, and other features It should be noted that it is not intended to preliminarily exclude the existence of elements, steps, functions, components, or combinations thereof.

On the other hand, unless otherwise defined, all terms used herein should be regarded as having the same meaning as commonly understood by those of ordinary skill in the art to which the present invention belongs. Accordingly, unless explicitly defined herein, specific terms should not be construed in an unduly idealistic or formal sense.

As used herein, the term "sample" can be of any type as long as it can contain a virus. In addition, the sample can be used in a non-purified or non-concentrated state before being put into the sample unit 100 according to the present invention, and if necessary, it is dissolved in a solution or buffer such as purified water to adjust the concentration or to make it into a liquid phase so that detection is easy. It may be desirable to pre-treat for ease. On the other hand, when the particles are too large depending on the sample, for example, when particles having a size of several tens or hundreds of μm or more are included, the large particles can be removed by filtering using an appropriate filter.

The present inventors have devised the following invention as a result of research in order to solve the above-mentioned problem.

<Virus detection device of the present invention>

The present specification includes a sample unit 100 for injecting a sample to be measured; a probe unit 200 for irradiating a broadband electromagnetic wave to the sample; a measuring unit 300 for measuring the complex permittivity of the sample with respect to the irradiated broadband electromagnetic wave; and a detection unit 400 for detecting a virus from the complex dielectric constant. Matters related to the virus detection method of the present invention are in accordance with the <Virus detection method of the present invention> described below. Hereinafter, in relation to the virus detection device of the present invention, it will be described in detail by configuration of each device.

1 schematically shows each configuration of a virus detection apparatus according to an embodiment of the present invention. Referring to FIG. 1 , the virus detection apparatus 10 according to an embodiment of the present invention includes a sample unit 100 for injecting a sample to be measured, a probe unit 200 for irradiating a broadband electromagnetic wave to the sample, and the irradiation. It can be seen that the measuring unit 300 for measuring the complex permittivity of the sample with respect to the broadband electromagnetic wave and the detecting unit 400 for detecting the virus from the complex permittivity are included.

In the virus detection device of the present invention, the sample unit 100 is responsible for injecting a sample to be measured, and while a specific virus (dielectric) in the sample to be detected is stored in the sample unit 100 for a certain period of time, a broadband electromagnetic wave is generated. It may be to provide a space to be irradiated.

The shape of the sample unit 100 of the present invention may be a cylinder, a plate, a cube, a cuboid, a regular polyhedron, a polygonal prism, or a sphere, but is not limited thereto.

In the virus detection apparatus of the present invention, the probe unit 200 plays a role of irradiating a broadband electromagnetic wave to the sample injected into the sample unit 100 of the present invention.

The probe unit 200 of the present invention may include an open-ended coaxial probe. The open coaxial line probe of the present invention can measure the reflection coefficient at the interface between the coaxial line probe and the sample, and more specifically, the complex permittivity spectra of a virus (dielectric) submerged in a liquid or liquid state. It has the advantage of being useful in some cases.

The diameter of the open coaxial probe of the present invention is preferably within the range of 1.0 to 10.0 mm, more preferably within the range of 1.5 to 3.0 mm. However, the diameter of the open coaxial line probe of the present invention may be changed according to the characteristics of the sample and is not limited to the above numerical range.

In addition, the length of the open coaxial line probe of the present invention is preferably within the range of 100 to 500 mm, but is not limited thereto.

Meanwhile, a wide frequency band of a radio-frequency band may be applied to the probe unit 200 of the present invention. More specifically, the frequency band of the broadband electromagnetic wave may be applied to the open coaxial line probe submerged in the sample.

The frequency of the broadband electromagnetic wave irradiated to the sample host to the sample unit 100 of the present invention is preferably within the range of 0.1 to 50 GHz, and more preferably within the range of 0.1 to 26.5 GHz.

In the virus detection apparatus of the present invention, the measuring unit 300 serves to measure the complex permittivity of the sample with respect to the irradiated broadband electromagnetic wave. More specifically, in the virus detection device of the present invention, the measuring unit 300 measures the complex permittivity spectra of the virus (dielectric) in the sample for a specific frequency band from the scattering parameter response (S-parameter response) characteristic. It may be to provide a space to be That is, in the measuring unit 300 of the present invention, the complex permittivity spectra of the virus (genome) may be extracted from the scattering parameter response characteristics. Since each specific virus (genome) has its own scattering parameter response characteristics, this means that each specific virus (genome) has its own complex permittivity spectra.

The measuring unit 300 of the present invention may include a vector network analyzer (VNA). A broadband electromagnetic wave irradiated to the sample is generated by the vector network analyzer, and the vector network analyzer may measure a scattering parameter (S-parameter) in a high frequency region. Here, the scattering parameter refers to a ratio of an input voltage to an output voltage at a specific frequency, and thus, physical properties such as a reflection coefficient and a transmission coefficient of a sample can be measured through a vector network analyzer. The vector network analyzer of the present invention may use a 1-port network system or a 2-port network system, but is not limited thereto.

2 is a view showing an overall configuration in which each configuration of the virus detection apparatus according to an embodiment of the present invention is organically combined. More specifically, FIG. 2A shows an open-ended coaxial probe corresponding to the probe unit 200 of the present invention and a vector network analyzer (VNA) corresponding to the measuring unit 300 of the present invention. ) shows the virus detection apparatus of the present invention, and FIG. 2B shows a detailed configuration of an open-ended coaxial probe corresponding to the probe unit 200 of the present invention.

Referring to FIG. 2A , in the virus detection device of the present invention, a broadband electromagnetic wave is irradiated to a sample from an open coaxial line probe connected to a vector network analyzer, scattering parameter response of a specific virus (genome) in the sample, complex permittivity spectra, etc. It can be confirmed that it can be measured.

In addition, referring to FIG. 2B , it can be seen that the detailed configuration of the open coaxial line probe of the present invention and the broadband electromagnetic wave from the open coaxial line probe of the present invention are irradiated to the sample to form an electric field.

3 is a photograph showing the actual state of the virus detection apparatus of the present invention including an open coaxial probe corresponding to the probe unit 200 of the present invention and a vector network analyzer corresponding to the measuring unit 300 of the present invention. will be.

3, in the virus detection device of the present invention, a broadband electromagnetic wave is irradiated to a sample from an open coaxial line probe connected to a vector network analyzer, scattering parameter response of a specific virus (genome) in the sample, complex permittivity spectra, etc. It can be confirmed that it can be measured.

Also, the measuring unit 300 of the present invention may measure a real part value, an imaginary part value, and a dielectric relaxation frequency for the complex dielectric constant of the sample. Here, the complex permittivity of the sample can be considered to mean the complex permittivity spectra of the virus (genome) in the sample.

The complex permittivity spectra of the virus (genome) in the sample measured by the measuring unit 300 of the present invention can be theoretically obtained from the following Equation (1).

where ε _r,0 is the static relative permittivity at frequency 0, ε _r,∞ is the relative permittivity at ω=∞, τ is the relaxation time, and α is the distribution (relaxation time) parameter. .

The real part value of the complex permittivity of the sample measured by the measuring unit 300 of the present invention is a value representing the degree of polarization of the virus (dielectric) in the sample when irradiating the sample with a broadband electromagnetic wave. If the sample is not irradiated with electromagnetic waves, the atoms of the virus (dielectric) maintain a spherical shape as the nucleus and electrons are equidistant from the sample. will be done In addition, in the polarization process, a dipole moment occurs and a torque is generated inside the atoms of the virus (dielectric). Since electrons are shared between the atoms of the virus (dielectric), friction occurs during the polarization process between them. do. The imaginary part value for the complex permittivity is a numerical value representing the loss of virus (dielectric) due to friction. Since the real part value and the imaginary part value for the complex permittivity have different values for each virus (genome), they can be used as a means of discriminating a specific virus (genome).

On the other hand, the dielectric relaxation frequency (f _c ) with respect to the complex permittivity is a value measured for the mobility of the virus (dielectric). When irradiating a broadband electromagnetic wave to a sample, when moving from low frequency to high frequency, the imaginary part of the complex permittivity increases and decreases based on the peak value. This frequency is called dielectric relaxation frequency. That is, when the value of the imaginary part of the complex permittivity is maximum, the corresponding frequency can be regarded as the dielectric relaxation frequency.

As a measurement value indicating the dielectric relaxation frequency and mobility of dipoles generated in the polarization process, the correlation between relaxation time (τ) is as shown in Equation 2 below. Similarly, since the dielectric relaxation frequency for the complex permittivity also has a different value for each virus (genome), it can be used as a means for discriminating a specific virus (genome).

Here, τ is the relaxation time (seconds), and f _c is the dielectric relaxation frequency (Hz).

The detection unit 400 of the present invention may detect the virus in the sample through a real part value, an imaginary part value, and a dielectric relaxation frequency for the complex dielectric constant of the sample. Here, the complex permittivity of the sample can be considered to mean the complex permittivity spectra of the virus (genome) in the sample.

Since the real part value, the imaginary part value, and the dielectric relaxation frequency of the complex permittivity of a specific virus (genome) are determined as intrinsic values, the known complex permittivity from the complex permittivity of the sample measured by the virus detection device of the present invention is compared. Virus (genome) can be specified. At this time, the dielectric relaxation frequency of the known complex permittivity can be obtained through Equation 2 above.

On the other hand, the display unit for displaying the virus in the sample detected by the detection unit 400 of the present invention on the display screen; may further include. The display unit of the present invention may display a detected specific virus together with a predetermined value or may display a currently set mode.

<Virus detection method of the present invention>

On the other hand, the present specification is a first step of injecting a sample to be measured; a second step of irradiating a broadband electromagnetic wave to the sample; a third step of measuring the complex permittivity of the sample with respect to the irradiated broadband electromagnetic wave; And a fourth step of detecting the virus from the complex dielectric constant; it further discloses a virus detection method comprising a. Matters overlapping with those relating to the virus detection device of the present invention will be omitted. Hereinafter, in relation to the virus detection method of the present invention, each method will be described in detail by step.

4 is a flowchart illustrating a virus detection method according to an embodiment of the present invention for each step. Referring to FIG. 4 , the virus detection method of the present invention includes a first step of injecting a sample to be measured (S100), a second step of irradiating the sample with a broadband electromagnetic wave (S200), and a complex sample with respect to the irradiated broadband electromagnetic wave. It can be seen that the third step (S300) of measuring the dielectric constant and the fourth step (S400) of detecting the virus from the complex dielectric constant are included.

In the first step of injecting a sample to be measured according to the present invention, a specific virus (genome) to be determined may be included in the sample.

In the second step of irradiating the broadband electromagnetic wave to the sample of the present invention, the broadband electromagnetic wave may be irradiated to the sample through the probe unit 200 of the present invention. Here, the probe unit 200 of the present invention may include an open-ended coaxial probe.

A wide frequency band of a radio-frequency band may be applied to the probe unit 200 of the present invention. More specifically, the frequency band of the broadband electromagnetic wave may be applied to the open coaxial line probe submerged in the sample.

The frequency of the broadband electromagnetic wave irradiated to the sample host to the sample unit 100 of the present invention is preferably within the range of 0.1 to 50 GHz, and more preferably within the range of 0.1 to 26.5 GHz.

In the third step of measuring the complex permittivity of the sample with respect to the irradiated broadband electromagnetic wave of the present invention, the complex permittivity spectra of the virus (dielectric) for a specific frequency band may be measured from the scattering parameter response.

In addition, in the third step of measuring the complex permittivity of the sample with respect to the irradiated broadband electromagnetic wave of the present invention, a real part value, an imaginary part value, and a dielectric relaxation frequency for the complex dielectric constant of the sample may be measured. Here, the complex permittivity of the sample can be seen as meaning the complex permittivity spectra of the virus (genome) in the sample.

The real part value of the complex dielectric constant of the sample measured in the third step of measuring the complex dielectric constant of the sample with respect to the irradiated broadband electromagnetic wave of the present invention is the polarization of the virus (dielectric) in the sample when the sample is irradiated with broadband electromagnetic waves ) is a numerical value indicating the degree. If the sample is not irradiated with electromagnetic waves, the atoms of the virus (dielectric) maintain a spherical shape as the nucleus and electrons are equidistant from the sample. will be done In addition, in the polarization process, a dipole moment occurs and a torque is generated inside the atoms of the virus (dielectric). Since electrons are shared between the atoms of the virus (dielectric), friction occurs during the polarization process between them. do. The imaginary part value for the complex permittivity is a numerical value representing the loss of virus (dielectric) due to friction. Since the real part value and the imaginary part value for the complex permittivity have different values for each virus (genome), they can be used as a means of discriminating a specific virus (genome).

On the other hand, the dielectric relaxation frequency (f _c ) for the complex permittivity is a value that measures the mobility of the virus (dielectric). When irradiating a broadband electromagnetic wave to the sample, the peak value is As a reference, the imaginary part of the complex permittivity increases and decreases. At this time, the frequency at which the reference peak value is located is called the dielectric relaxation frequency. That is, when the value of the imaginary part of the complex dielectric is maximum, the frequency can be regarded as a dielectric relaxation frequency.

In the fourth step of detecting the virus from the complex dielectric constant of the present invention, the virus in the sample can be detected through the real part value, the imaginary part value, and the dielectric relaxation frequency of the complex dielectric constant of the sample measured in the third step. Here, the complex permittivity of the sample can be considered to mean the complex permittivity spectra of the virus (genome) in the sample.

Since the real part value, the imaginary part value, and the dielectric relaxation frequency of the complex permittivity of a specific virus (genome) are determined as intrinsic values, from the complex permittivity of the sample measured in the virus detection method of the present invention, the known complex permittivity is compared. Virus (genome) can be specified. At this time, the dielectric relaxation frequency of the known complex permittivity can be obtained through Equation 2 above.

In addition, the virus detection method of the present invention may further include; a fifth step of displaying the detected virus on a display screen (not shown). The fifth step in the virus detection method of the present invention may include displaying a detected specific virus with a predetermined value or displaying a currently set mode.

Hereinafter, with reference to the accompanying drawings and embodiments will be described in more detail with respect to what the present specification claims. However, the drawings and examples presented in this specification may be modified in various ways by those skilled in the art to have various forms, and the description of the present specification is not intended to limit the present invention to a specific disclosed form. It should be considered to include all equivalents or substitutes included in the spirit and scope of the present invention. In addition, the accompanying drawings are presented to help those of ordinary skill in the art to more accurately understand the present invention, and may be exaggerated or reduced than in reality.

{Examples and Evaluation}

Experimental Example 1. Measurement of complex permittivity using the virus detection device of the present invention

The nucleoprotein gene of RSV (Respiratory syncytial virus) was cloned, and the cloned RSV nucleoprotein gene was expressed in Escherichia coli and purified at a concentration of 100 μg/mL in DI water (hereinafter referred to as 'RSV rNP'). ).

A monoclonal antibody targeting RSV rNP was bound to RSV rNP and purified at a concentration of 100 μg/mL in DI water (hereinafter referred to as 'RSV rNP + Antibody')

Bovine serum albumin (BSA), which corresponds to a negative control, was purified in DI water at a concentration of 100 μg/mL (hereinafter referred to as 'BSA').

The purified RSV rNP, RSV rNP + Antibody, and BSA are each injected into the sample unit 100 of the present invention in the state of an aqueous solution, and the frequency of the broadband electromagnetic wave has an operating bandwidth within the range of 0.1 to 26.5 GHz of the present invention The complex permittivity of each sample was measured in a virus detection device. At this time, the broadband electromagnetic wave was irradiated through the probe unit 200 of the present invention, and the complex permittivity of each sample with respect to the irradiated broadband electromagnetic wave was measured through the measuring unit 300 of the present invention. In addition, the virus was detected from the complex dielectric constant of each sample measured through the detection unit 400 of the present invention.

Evaluation 1. Detection of virus through the virus detection device of the present invention

5 is a diagram illustrating an experiment for measuring the complex permittivity of a sample in an aqueous solution state by using the virus detection apparatus according to an embodiment of the present invention.

5, RSV rNP, RSV rNP + Antibody and BSA purified according to Experimental Example 1 were injected into the sample unit 100 of the present invention in an aqueous solution state, respectively, corresponding to the probe unit 200 of the present invention. It can be seen that a broadband electromagnetic wave is irradiated through an open coaxial line probe.

6 is a graph showing the scattering parameter response characteristics of a sample in an aqueous solution state using the virus detection apparatus according to an embodiment of the present invention.

Referring to FIG. 6 , it can be seen that the magnitude and phase characteristics of the scattering parameter responses to RSV rNP, RSV rNP + Antibody, and BSA purified according to Experimental Example 1 are significantly different from each other.

As described above, the complex permittivity spectra of the virus (genome) can be extracted from the scattering parameter response characteristics. Since each specific virus (genome) has its own scattering parameter response characteristics, this means that each specific virus (genome) has its own complex permittivity spectra.

7 is a graph showing the complex dielectric constant spectra of a sample in an aqueous solution state by using the virus detection apparatus according to an embodiment of the present invention. More specifically, FIG. 7a is a graph showing the measurement of the real part value of the complex permittivity according to the frequency of broadband electromagnetic waves for RSV rNP, RSV rNP + Antibody, and BSA purified according to Experimental Example 1, and FIG. 7b is a graph showing the The imaginary part of the complex permittivity according to the frequency of broadband electromagnetic waves for RSV rNP, RSV rNP + Antibody, and BSA purified according to Experimental Example 1 was measured and shown as a graph. For reference, FIG. 7 includes complex permittivity spectra of DI water.

Referring to FIG. 7 , it can be seen that the complex permittivity spectra of RSV rNP, RSV rNP + Antibody, and BSA purified according to Experimental Example 1 are significantly different from each other.

First, comparing the complex permittivity spectra of BSA and DI water, the real part value (ε _r ') of the complex permittivity spectra of BSA is 63 at 1 GHz, and this value is 14 smaller than the value of DI water at the same frequency. This result is because the degree of polarization was reduced by the BSA molecule. _{In addition, from the result of the imaginary part value (ε r} ") of the complex permittivity spectra of BSA in the frequency range of interest, it can be confirmed that the loss due to friction caused by the polarization process is smaller than that of DI water. Furthermore, the dielectric relaxation of BSA the frequency (f _c) is 20.9 GHz, and the dielectric relaxation frequency of DI water (f _c) and the value is very similar, but the complex dielectric constant of the imaginary part values of the spectra of the BSA in a 20.9 GHz frequency (ε _r ") is about 32 , and this value is smaller by 4 compared to the value of DI water at the same frequency.

Comparing the complex dielectric constant spectra of RSV rNP with BSA, 'and 66, the complex dielectric constant real part values of the spectra of BSA (ε _r at 1GHz real part value of the complex dielectric constant spectra of RSV rNP (ε _r)') is 63 . Referring to FIG. 7A , it can be seen that the difference between the real part values of the complex permittivity spectra remains the same in the frequency band of 1 to 26.5 GHz.

_{In addition, the real part value (ε r} ') of the complex permittivity spectra of RSV rNP + Antibody is 78 at 1 GHz, which is 12 higher than the value of RSV rNP at the same frequency. Referring to FIG. 7A , it can be seen that the difference between the real part values of the complex permittivity spectra remains the same in the frequency band of 1 to 26.5 GHz. The reason that the real part value of the complex permittivity spectra of RSV rNP + Antibody (ε _r ') is larger than the real part value of the complex permittivity spectra of RSV rNP (ε _r ') is the antigen-antibody interaction between RSV rNP antigen and RSV rNP antibody. This is because the action produced another biological material as a by-product. _{On the other hand, the low-frequency dispersion of the real part value (ε r} ') of the complex permittivity spectra of RSV rNP and RSV rNP + Antibody in the frequency band below 0.6 GHz is caused by the different permittivity and conductivity of the bio-particles and the surrounding medium.

On the other hand, through FIG. 7B, _{not only the real part value (ε r} ') of the complex permittivity spectra, but also the imaginary part values (ε _r ") of the complex permittivity spectra of RSV rNP, RSV rNP + Antibody and BSA are significantly distinguished. As described above, the imaginary part value (ε _r ") of the complex permittivity spectra is a value representing the loss due to friction caused by the polarization process of the virus (dielectric) when the sample is irradiated with broadband electromagnetic waves, There are two types of dielectric loss mechanisms: dipole loss (ε _rd ") and ionic loss (ε _{rσ ").} The total dielectric loss can be calculated simply by adding the dipole loss and the ion loss as shown in Equation 3 below.

where ε _r ″ is the total dielectric loss, ε _rd ″ is the dipole loss, and ε _rσ ″ is the ion loss.

Referring to Figure 7b, RSV rNP, RSV rNP + Antibody _{can confirm the predominance of ionic loss (ε rσ} ") of less than 1 GHz. This dominant tendency is a nucleic acid (DNA or RNA) ), which is typically observed in biological substances related to , that is, from this, it can be confirmed that RSV rNP is structurally related to nucleic acids.

On the other hand, BSA has negligible ionic loss (ε _rσ "), but dipole loss (ε _rd ") contributes to the total dielectric loss of BSA. The reason BSA has little ionic loss (ε _rσ ") is that BSA is composed of oval protein molecules containing 607 amino acids and alanine.

_{This tendency of} ionic loss (ε rσ ") and dipole loss (ε _rd ") is that viral materials cause ion losses due to NPs, whereas non-viral materials cause little ion loss and dipole losses. _{It can be confirmed that the imaginary part value (ε r} ") of the complex permittivity spectra of the virus (genome) also serves as a major indicator for virus detection.

Therefore, it can be confirmed that RSV rNP, RSV rNP + antibody can be distinguished from BSA in _{ionic loss (ε rσ} ") from these two tendencies of ion loss and dipole loss.

In addition, as described above, since each virus (genome) has a unique dielectric relaxation frequency (f _c ), three substances from each of the inducing agent relaxation frequencies of RSV rNP, RSV rNP + Antibody and BSA in Table 1 below can also be distinguished.

sample material	Dielectric relaxation frequency (f c , GHz)
RSV rNP + Antibody	18.6
RSV rNP	22.7
BSA	20.9

As described above, the dielectric relaxation frequency (f _c ) is a measure indicating the mobility of dipoles generated in the polarization process of a virus (genome) and is related to the relaxation time (τ). Referring to FIG. 7b , it can be confirmed that the peak value of RSV rNP is reduced than the peak value of DI water. This is presumably because a significant amount of water molecules bind to NPs, which are strongly charged nucleic acids. According to this tendency, the dielectric relaxation frequency (f _c ) and relaxation time (τ) have different values for each virus (genome). That is, the genome relaxation frequency (f _c ) of the virus (genome) can also serve as a key indicator for virus detection.

As such, the virus detection apparatus of the present invention using genome spectroscopy is a technology using the principle that each virus (genome) has its own complex permittivity spectra, and unlike fluorescent antibody labeling technology, it does not go through a separate labeling process. It has the effect of increasing the convenience and speed of the

The virus detection apparatus and the detection method of the present invention according to the present invention can detect a specific virus quickly and accurately by measuring the complex dielectric constant spectra of the virus through an electrochemical method even with a small amount of sample.

The above description is merely illustrative of the technical spirit of the present invention, and various modifications and variations will be possible without departing from the essential characteristics of the present invention by those skilled in the art to which the present invention pertains.

Accordingly, the embodiments disclosed in the present invention are not intended to limit the technical spirit of the present invention, but to explain, and the scope of the technical spirit of the present invention is not limited by these embodiments. The protection scope of the present invention should be construed by the following claims, and all technical ideas within the scope equivalent thereto should be construed as being included in the scope of the present invention.

Claims (10)

1. a sample unit for injecting a sample to be measured;

   a probe unit irradiating a broadband electromagnetic wave to the sample;

   a measuring unit measuring the complex permittivity of the sample with respect to the irradiated broadband electromagnetic wave; and

   A virus detection device comprising a; a detection unit for detecting the virus from the complex dielectric constant.
2. The method of claim 1,

   The probe unit includes an open-ended coaxial probe (open-ended coaxial probe), virus detection device.
3. The method of claim 1,

   The frequency of the broadband electromagnetic wave is characterized in that within the range of 0.1 to 50 GHz, virus detection device.
4. According to claim 1,

   The measuring unit comprises a vector network analyzer (Vector network analyzer), virus detection device.
5. According to claim 1,

   Wherein the measuring unit measures a real part value, an imaginary part value, and a dielectric relaxation frequency with respect to the complex permittivity of the sample, the virus detection device.
6. According to claim 1,

   The detection unit, the virus detection device, characterized in that for detecting the virus in the sample through a real part, an imaginary part, and a dielectric relaxation frequency with respect to the complex dielectric constant of the sample.
7. According to claim 1,

   The virus detection device further comprising; a display unit for displaying the detected virus on a display screen.
8. A first step of injecting a sample to be measured;

   a second step of irradiating a broadband electromagnetic wave to the sample;

   a third step of measuring the complex permittivity of the sample with respect to the irradiated broadband electromagnetic wave; and

   A virus detection method comprising a; a fourth step of detecting the virus from the complex dielectric constant.
9. 9. The method of claim 8,

   The frequency of the broadband electromagnetic wave is characterized in that within the range of 0.1 to 50 GHz, virus detection method.
10. 9. The method of claim 8,

    A fifth step of displaying the detected virus on a display screen; further comprising, a virus detection method.

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