WO2021244467A1

WO2021244467A1 - Air purification assembly having coronavirus killing function and application thereof

Info

Publication number: WO2021244467A1
Application number: PCT/CN2021/097165
Authority: WO
Inventors: 王丹; 丁浩旻; 王洁欣; 孙敏; 孙宝昌; 曾晓飞; 初广文; 陈建峰
Original assignee: 北京化工大学; 北京鼎卫科技服务有限公司
Priority date: 2020-06-01
Filing date: 2021-05-31
Publication date: 2021-12-09
Also published as: CN111729119A

Abstract

Disclosed are an air purification assembly having a coronavirus killing function and an application thereof. The air purification assembly comprises a first support layer, a second support layer, and a functional material layer disposed between the first support layer and the second support layer; the functional material layer comprises at least one organic-inorganic composite copper fiber material layer and at least one particulate matter filtering layer. The present invention further provides the application of the air purification assembly in manufacturing of an air purification device.

Description

An air purification component with the function of killing coronavirus and its application

Technical field

The invention relates to the cross-technology field of materials, chemical engineering and life sciences. More specifically, it relates to an air purification component with a function of killing coronavirus and its application.

Background technique

During the 2020 novel coronavirus pneumonia epidemic, how to effectively block the spread of the virus between people and the environment has become an important issue for epidemic prevention and control.

Masks worn by ordinary people, air purifiers and air conditioners used in indoor places can all play a role in purifying the air. Such air purification products can filter out pathogenic microorganisms, dust, smoke, fog and other particulate matter. The components are usually fibrous materials with a porous structure. Through the electrostatic adsorption of the fibers and the tiny gaps in the arrangement of the fibers, the barriers to pathogenic microorganisms and other particles are realized. However, because the coronavirus can survive for 2-3 days on the surface of high molecular polymer plastics, the coronavirus that is intercepted on the surface of the filter materials of end products such as masks, air purifiers, air conditioners, etc., may desorb and spread again Into the environment, it brings potential biological risks to users. Therefore, air purification materials that can effectively kill the coronavirus are expected to reduce the risk of virus accumulation during long-term use of air purification products, and provide safer air purification and virus protection effects.

At present, a study published in the "New England Journal of Medicine" shows that the new coronavirus (SARS-CoV-2) can survive up to 4 hours on metallic copper surfaces, which is significantly smaller than other solid surfaces (such as cardboard surfaces for up to 24 hours). Hours, it can survive for up to 2-3 days on plastic and stainless steel surfaces). In fact, the inhibitory effect of copper on bacteria and microorganisms has long attracted the attention of academia and industry. Li Shizhen’s "Compendium of Materia Medica" uses patina (green basic copper carbonate produced by the reaction of copper with oxygen, carbon dioxide, and water vapor in the air, also known as bronze) as a type of traditional Chinese medicine, which can be used to treat malnutrition and malnutrition. Bacterial infections such as bayberry sore, ecthyma and stubborn ringworm. Modern science has proposed a variety of scientific explanations for the antibacterial mechanism of copper ions. For example, copper can accept and release electrons to ^{convert between Cu +} and Cu ²⁺ , and can be used as a catalyst to generate reactive oxygen species (ROS), which can lead to the survival of bacteria. Oxidative damage to important cellular components such as proteins, nucleic acids and lipids (including cell membranes). Copper ions may also compete with Zn ²⁺ or other metal ions for protein binding sites. This wrong combination leads to changes in protein conformation, which leads to protein dysfunction and causes bacterial cell apoptosis.

In recent years, by combining metal ion copper materials with polymer fibers, the prepared copper ion fiber materials have been used in textiles such as towels, socks, underwear, and mattresses. Copper fibers can kill bacteria that are concentrated on the surface of such textiles or penetrate into the interior, and have the effects of antibacterial, antibacterial, and sterilization. However, the inventor found that the prior art has at least the following problems: the existing copper fiber sterilization materials used in towels, socks and other textiles are difficult to meet the integration and compatibility with air purification end products such as masks, air purifiers, and air conditioners. Effective barrier and killing of coronavirus. At the biological level, copper nanofibers can catalyze the production of reactive oxygen species or change protein conformation and other process mechanisms, so that the coronavirus loses its infection function. However, because the diameter of the coronavirus is generally within 0.1 μm, in application scenarios such as masks, air purifiers, and air conditioners, such small-sized virus particles usually pass through the copper fiber material with the airflow at a certain speed. It is difficult to be adsorbed on the surface of copper fiber by means of mechanical filtration such as sedimentation, inertial collision, interception, diffusion, etc., and the doping of copper ions weakens the electrostatic adsorption capacity of polymer fibers, which makes it difficult for the filter efficiency of 0.1 micron particles to meet the requirements of masks. And other air purification products require the filtration efficiency of the filter material. Therefore, although copper fiber products can effectively adsorb and kill large-sized bacteria in relatively static use scenarios (such as towels, underwear, socks, and mattresses), their adsorption and absorption of ultra-small-sized microorganisms such as coronaviruses is very effective. The killing is restricted, and it is difficult to apply to air purification end products such as masks, air purifiers, and air conditioners.

Therefore, the present invention provides an air purification component with the function of killing coronavirus and its application, so as to solve at least one of the above-mentioned problems.

Summary of the invention

An object of the present invention is to provide an air purification module with the function of killing the coronavirus.

Another object of the present invention is to provide an application of an air purification module with the function of killing the coronavirus.

In order to achieve the above objectives, the present invention adopts the following technical solutions:

In a first aspect, the present invention provides an air purification assembly with a function of killing coronaviruses, including a first support layer, a second support layer, and a functional material layer arranged between the first support layer and the second support layer; The functional material layer includes at least one organic-inorganic composite copper fiber material layer and at least one particulate filter layer.

Preferably, the particulate filter layer is provided between the organic-inorganic composite copper fiber material layer and the first support layer, or between the organic-inorganic composite copper fiber material layer and the second support layer .

Preferably, the functional material layer includes multiple organic-inorganic composite copper fiber material layers and at least one particulate filter layer.

Preferably, the particulate filter layer is arranged between multiple layers of organic-inorganic composite copper fiber material.

Preferably, the number of layers of the organic-inorganic composite copper fiber material layer is two.

Preferably, the number of layers of the particulate filter layer is one layer.

Preferably, the functional material layer includes two organic-inorganic composite copper fiber material layers and a particulate filter layer arranged between the two organic-inorganic composite copper fiber material layers.

Preferably, the average fiber diameter of the organic-inorganic composite copper fiber material in the organic-inorganic composite copper fiber material layer is 0.01-20 μm.

Preferably, the organic-inorganic composite copper fiber material is a composite fiber material prepared by a composite method of polymer and copper; wherein the composite method includes nano-master batch method, melt blending method, solution blending method, spray steaming One or more of plating method, surface finishing method and graft modification method; the polymer is preferably polyethylene terephthalate, polypropylene, polyurethane, polytetrafluoroethylene, polyvinylidene fluoride and One or more of cotton fiber.

Preferably, the mass fraction of copper in the organic-inorganic composite copper fiber material is 0.01-10%.

Preferably, the material of the organic-inorganic composite copper fiber material layer includes polyethylene terephthalate composite copper fiber cloth, polypropylene composite copper fiber cloth, polyurethane composite copper fiber cloth, and polytetrafluoroethylene composite copper fiber cloth One or more of composite copper fiber cloth with cotton.

Preferably, the material of the particulate filter layer includes polypropylene meltblown non-woven fabric, polyurethane nanofiber membrane, polytetrafluoroethylene nanofiber membrane, polyvinylidene fluoride nanofiber membrane, polypropylene nanofiber membrane and polyterephthalene. One or more of ethylene formate nanofiber membranes.

Preferably, the materials of the first support layer and the second support layer respectively include one of polypropylene fiber cloth, cotton fiber cloth, polyurethane fiber cloth, polyvinylidene fluoride fiber cloth and polytetrafluoroethylene fiber cloth Or multiple.

In the second aspect, the present invention provides an application of the above-mentioned air purification assembly with the function of killing coronaviruses in the preparation of air purification devices.

In a third aspect, the present invention provides an air purification device including the above-mentioned air purification assembly with the function of killing the coronavirus.

The air purification device described in the present invention includes a mask, an air purifier or an air conditioner, etc.

The beneficial effects of the present invention are as follows:

The air purification component provided by the present invention can simultaneously realize the functions of air purification and killing the coronavirus, and provide technical means for realizing the high performance of air purification terminal products such as masks, air purifiers, and air conditioners.

Description of the drawings

In order to explain the technical solutions in the embodiments of the present invention more clearly, the following will briefly introduce the accompanying drawings used in the description of the embodiments. Obviously, the accompanying drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative work.

Figure 1 shows one of the structural schematic diagrams of the air purification module with the function of killing coronaviruses provided by the present invention;

Figure 2 shows one of the structural schematic diagrams of the air purification assembly with the function of killing the coronavirus provided by the present invention;

Figure 3 shows one of the structural schematic diagrams of the air purification module with the function of killing the coronavirus provided by the present invention;

Figure 4 shows a scanning electron micrograph of a polyethylene terephthalate composite copper fiber cloth provided by the present invention;

Among them, 1-first support layer, 2-functional material layer, 21-organic-inorganic composite copper fiber material layer, 22-particle filter layer, 3-second support layer.

detailed description

In order to make the technical solutions and advantages of the present invention clearer, the following further describes the embodiments of the present invention in detail with reference to the accompanying drawings.

The raw materials used in the present invention can be obtained from publicly disclosed commercial channels unless otherwise specified.

In view of the shortcomings of the existing copper ion fiber materials for blocking and killing coronaviruses, the present invention proposes a multi-layer functional film laminate structure component with a particulate filter layer, an organic-inorganic composite copper fiber material layer and a support layer, as a mask, The filter elements of products such as air purifiers and air conditioners can filter, adsorb and kill coronavirus particles in a dynamic environment.

On the one hand, an embodiment of the present invention provides an air purification assembly with a function of killing coronaviruses, as shown in FIG. 1, including:

The first supporting layer 1, the second supporting layer 3, and the functional material layer 2 arranged between the first supporting layer 1 and the second supporting layer 3; wherein the first supporting layer 1 and the second supporting layer 3 are both Used to fix the structure of the air purification component, the functional material layer 2 has the functions of killing the coronavirus and purifying the air;

The functional material layer 2 includes at least one organic-inorganic composite copper fiber material layer 21 and at least one particulate filter layer 22;

The organic-inorganic composite copper fiber material layer 21 has the functions of inactivating the new coronavirus and filtering particles;

The particulate filter layer 22 has the function of intercepting and/or adsorbing viral particles and other particulates, and its filtration efficiency for ^{sodium chloride particles with a size of 0.055-0.095μm at a concentration of 0.001-200mg/m 3 air flow is} 90-99.9%;

The organic-inorganic composite copper fiber material layer 21 and the particulate filter layer 22 cooperate with each other and work together to not only kill the coronavirus, but also further improve the degree of air purification.

In a specific embodiment of the present invention, as shown in Figures 1 and 2, the particulate filter layer 22 is provided between the organic-inorganic composite copper fiber material layer 21 and the first support layer 1, or is provided Between the organic-inorganic composite copper fiber material layer 21 and the second supporting layer 3. In addition, the particulate filter layer 22 and the organic-inorganic composite copper fiber material layer 21 shown in FIGS. 1 and 2 are both single layers. It should be understood that the particulate filter layer 22 and the organic-inorganic composite copper fiber material layer 21 can both be single layers. The layer may also be multiple layers, which does not limit the protection scope of the present invention.

In order to further enhance the effect of killing the coronavirus, the functional material layer 2 includes multiple organic-inorganic composite copper fiber material layers 21 and at least one particulate filter layer 22. The invention has been confirmed by research that the multi-layer organic-inorganic composite copper fiber material layer has a better killing effect on the coronavirus.

In a specific embodiment of the present invention, the particulate filter layer 22 is provided between the multiple organic-inorganic composite copper fiber material layers 21. It should be understood that the particulate filter layer may be integrally provided between certain two organic-inorganic composite copper fiber material layers in the multilayer organic-inorganic composite copper fiber material layer, or it may be a multilayer particulate filter layer and a multilayer organic-inorganic composite copper fiber material layer. Layers of copper fiber material are alternately arranged. The present invention has been confirmed by research that the outside air first passes through the organic-inorganic composite copper fiber material layer to kill viruses and particulate filtration, then passes through the particulate filter layer, and then passes through the organic-inorganic composite copper fiber material layer to kill and filter, and the final virus kills The killing effect and air purification effect are the best compared with other solutions.

Further, the number of layers of the organic-inorganic composite copper fiber material layer 21 is two.

Further, the number of layers of the particulate filter layer 22 is one layer.

Considering the killing of coronavirus, air purification, gas stuffiness, and manufacturing cost, as a more preferred embodiment, as shown in FIG. 3, the functional material layer 2 includes two organic-inorganic composite copper fiber material layers 21 and equipment. A particulate filter layer 22 between the two organic-inorganic composite copper fiber material layers 21.

As a preferred embodiment of the present invention, the organic-inorganic composite copper fiber material in the organic-inorganic composite copper fiber material layer is an organic-inorganic composite fiber material doped with copper, and the average fiber diameter of the fiber is 0.01-20 μm. The filtration efficiency of sodium chloride particles with a concentration of 0.001 to 200 mg/m ^{3 and an} air flow rate of 0.055 to 0.095 μm is 30 to 99.9%.

As a preferred embodiment of the present invention, the organic-inorganic composite copper fiber material is a composite fiber material prepared by a composite method of polymer and copper; wherein the composite method includes nano master batch method, melt blending method, solution One or more of blending method, spray evaporation method, surface finishing method, and graft modification method; it should be understood that the aforementioned composite method may be a conventional method, and will not be repeated here.

Further, the polymer is selected from one or more of polyethylene terephthalate, polypropylene, polyurethane, polytetrafluoroethylene, polyvinylidene fluoride and cotton fiber.

In addition, after analyzing the effect and cost of killing the coronavirus, the mass fraction of copper in the organic-inorganic composite copper fiber material is preferably 0.01-10%.

As a preferred embodiment of the present invention, the material of the organic-inorganic composite copper fiber material layer includes polyethylene terephthalate composite copper fiber cloth, polypropylene composite copper fiber cloth, polyurethane composite copper fiber cloth, and polytetrafluoroethylene composite copper fiber cloth. One or more of fluoroethylene composite copper fiber cloth and cotton composite copper fiber cloth.

As a preferred embodiment of the present invention, the material of the particulate filter layer includes polypropylene meltblown non-woven fabric, polyurethane nanofiber film, polytetrafluoroethylene nanofiber film, polyvinylidene fluoride nanofiber film, polypropylene nanofiber One or more of membranes and polyethylene terephthalate nanofiber membranes; particle filtration test results show that this type of preferred material has a filtration efficiency of more than 90% for 0.1 micron sodium chloride particles, which can effectively block The spread of particulate matter.

As a preferred embodiment of the present invention, the materials of the first support layer and the second support layer respectively include polypropylene fiber cloth, cotton fiber cloth, polyurethane fiber cloth, polyvinylidene fluoride fiber cloth and polytetrafluoroethylene One or more of the fiber cloths provide structural support for air purification components.

In the second aspect, an embodiment of the present invention provides an application of the above-mentioned air purification component with the function of killing coronaviruses in the preparation of an air purification device.

In a third aspect, an embodiment of the present invention provides an air purification device including the above-mentioned air purification component with the function of killing coronavirus.

The air purification device described in the embodiment of the present invention includes a mask, an air purifier, an air conditioner, and the like.

In summary, the support layer in the present invention provides structural support for air purification components and has high mechanical strength and porosity; the particulate filter layer plays a role in intercepting and adsorbing virus particles; the organic-inorganic composite copper fiber material layer is mainly used for Coronavirus inactivation; the three layers of materials are organically combined and applied to air purification terminal products such as masks, air purifiers, and air conditioners to achieve air purification and the elimination of coronaviruses.

The present invention also provides the following examples of testing the air purification component and the air purification device containing the same to kill the coronavirus.

Example 1

This embodiment provides an air purification assembly with a function of killing coronaviruses. As shown in FIG. 3, it includes a first support layer 1, a second support layer 3, and are arranged on the first support layer 1 and the second support layer 3 between the functional material layer 2; wherein, the functional material layer 2 includes two organic-inorganic composite copper fiber material layers 21 and a particulate filter layer 22 arranged between the two organic-inorganic composite copper fiber material layers 21 ；

The material of the particulate filter layer 22 is polypropylene meltblown non-woven fabric, which is used to intercept and adsorb coronavirus particles;

The material of the organic-inorganic composite copper fiber material layer 21 is a polyethylene terephthalate composite copper fiber cloth with ^{a copper content of about 350 mg/m 2.} The diameter is 13 microns, which is used to inactivate the coronavirus;

The first supporting layer and the second supporting layer are respectively polypropylene fiber membranes, which have structural stability and provide structural support for the air purification component.

Example 2

This embodiment provides a method for in vitro experiments against the H1N1 virus, in which the air purification component with the function of killing coronaviruses provided in embodiment 1 is used as the test material, and the polyterephthalic acid in embodiment 1 is used as the test material. The air purification component made by replacing the ethylene glycol ester composite copper fiber cloth with pure polyethylene terephthalate fiber cloth without copper is used as a control material, which specifically includes the following steps:

(1) Sterile 96-well culture plate, add 100μl ^{MDCK cells at a concentration of 5×10 4} cell/ml to each well, and incubate at 37°C with 5% CO _{2 for} 24 hours;

(2) The test material and the control material are sterilized by ultraviolet for 30 minutes, and the area of the test material and the control material are respectively cut to 20mm×20mm, and then cut into 3mm×3mm fragments and placed in a 15ml sterile centrifuge tube. The control group 2 and 9 in the experimental group.

(3) Prepare 10 ⁷ TCID ₅₀ /ml virus solution, and add 2 ml of each to 18 centrifuge tubes with different filter materials. The virus liquid was incubated with the material at 25°C, and the action time was 0, 1 and 18h, respectively. Three tubes were set up for each time point of the test material and the control material.

(4) After the action time, transfer the mixed material liquid to a 50ml centrifuge tube, add MEM medium (containing 2% bi-antibody and 2μg/ml trypsin) to 20ml, and shake to elute virus particles;

(5) Use MEM medium (containing 2% double antibody and 2μg/ml trypsin) to perform a 10-fold gradient dilution of the eluate, and the dilutions are respectively original times, 10 ^-1 , 10 ^-2 , ... 10 ^-7 A total of 8 dilutions;

(6) Discard the cell culture medium in the 96-well culture plate, add the diluted virus eluate, 100μ1 per well, 8 replicate wells per concentration, and then add 100μ1 MEM medium (2% double antibody and 2μg/well) to each well. ml trypsin);

(7) Establish cell control and virus control at the same time; cell control: each well of 200μ1 MEM medium (containing 2% double antibody and 2μg/ml trypsin) is added to the monolayer of the 96-well culture plate of MDCK cells, a total of 4 Multiple holes. Virus control: each well of 100μ1 MEM medium (containing 2% double antibody and 2μg/ml trypsin), then add an equal volume of 100TCID ₅₀ virus, a total of 4 multiple wells, 37 ℃ for 1h, after 1h discarded in the 96-well culture plate Cell culture medium, add the above-mentioned virus liquid.

(8) Incubate in a 37°C 5% CO ₂ incubator for 3 days;

(9) After 3 days, 50 μl of the cell culture supernatant per well was added to a 96-well V bottom plate, and an equal volume of 1% turkey blood was added to each well for detection. After 30 minutes of adsorption, the 96-well plate was erected at 90 degrees. If the blood cells did not flow down or were consistent with the virus control well, the results were judged as positive, and the blood cells flow down or the results consistent with the cell control wells were judged as negative.

Anti-virus effect calculation:

1) Calculation of reduction value M:

M=lg(Va/Vb);

In the formula, Va represents the average value of the virus titer of the control material 0h eluted virus liquid;

Vb represents the average value of the virus titer of the eluted virus solution after the control material has been in contact for a certain period of time.

2) Calculation of antiviral activity value Mv:

Mv=lg(Vb/Vc);

In the formula, Vb represents the average value of the virus titer of the eluted virus solution after the control material has been in contact for a certain period of time; Vc represents the average value of the virus titer of the eluted virus solution after the test material has been in contact for a certain period of time.

3) Calculation of killing rate:

Killing rate = (Vb-Vc)/Vb×100%;

The test results are shown in Table 1 and Table 2:

Table 1 1h antiviral activity and killing rate of materials

Table 2 18h antiviral activity and killing rate of materials

The results show that the air purification module provided by the present invention has a killing rate of >50% for the H1N1 virus after acting for 1 hour, and has a killing rate of over 99% for the H1N1 virus after acting for 18 hours.

Example 3

This embodiment provides a method for in vitro experiments against coronavirus GX_P2V, in which the air purification component with the function of killing the coronavirus provided in embodiment 1 is used as the test material, and the polyterephthalene in embodiment 1 is used as the test material. The air purification component made by replacing the ethylene formate composite copper fiber cloth with pure polyethylene terephthalate fiber cloth without copper is used as the control material, which specifically includes the following steps:

(1) After UV sterilization of the two materials for 30 minutes, cut the test material and the control material 20mm×20mm, then cut into 3mm×3mm fragments and put them in a 15ml sterile centrifuge tube, of which 9 in the control material group , The numbers are: C1, C2, C3, C4, C5, C6, C7, C8, C9; 6 test material groups, the numbers are: D1, D2, D3, D4, D5, D6.

(2) Prepare 106PFU/ml GXP2V virus suspension, and add 2ml of each to 15 centrifuge tubes with different filter materials. Incubate the virus suspension with the filter material at room temperature, and the action time is as follows: control material group C1C2C3-15min, control material group C4C5C6-18h, control material group C7C8C9-instantaneous incubation (<10s), test material group D1D2D3-15min, test material Group C4C5C6-18h.

(3) Configure SCDLP liquid medium and autoclave. After the virus and the filter material are incubated for the specified time, add 20ml of SCDLP medium to each tube, shake and wash the virus particles, and perform a ten-fold gradient of the SCDLP medium containing virus particles with the high-sugar DMEM medium containing 10% FBS Dilute to obtain working fluids with six dilutions of ^{10 -1} , 10 ^-2 , 10 ^-3 , 10 ^-4 , 10 ^-5 , and 10 ^-6.

(4) Prepare Vero E6 cells in a 96-well plate. After changing the cells, add 100ul of different dilutions of different groups of working solutions, incubate at 37°C with 5% CO _{2 for 2} hours, continue to incubate for 72 hours after changing the medium, observe the CPE under a microscope, and perform qPCR detection after nucleic acid extraction. .

(5) Prepare a 6-well plate with Vero E6 cells. After the cells are exchanged, add 1ml of working solutions of different dilutions of different groups, incubate at 37°C with 5% CO _{2 for 2} hours, and continue to incubate for 72 hours after changing the medium. Observe the plaques by staining with crystal violet.

The test results are shown in Table 3:

Table 3 Action time and result of control material and test material

The results show that: the test material has 15 minutes of treatment, the coronavirus GX_P2V killing rate is >99%, and the inactivation log value is ≥3; the test material is used for 18 hours, the coronavirus GX_P2V killing rate is >99%, and the inactivation log value is ≥3; The logarithmic reduction of infection for 15min and 18h after the material was applied was ≤1.

Example 4

This example provides a method for in vitro experiments against the new coronavirus SARS-CoV-2 virus, in which the air purification component with the function of killing the coronavirus provided in Example 1 is used as the test material, and the test material is used in Example 1. The air purification components made by replacing the polyethylene terephthalate composite copper fiber cloth with pure polyethylene terephthalate fiber cloth without copper are used as the control material, which specifically includes the following steps:

(1) Sterile 96-well culture plate, add 100μl of ^{Vero E6 cells at a concentration of 5×10 4} cell/ml to each well, and incubate at 37°C with 5% CO _{2 for} 24 hours;

(4) After the action time, transfer the mixed material liquid to a 50ml centrifuge tube, add DMEM medium (containing 2% bi-antibody and 16μg/ml trypsin) to 20ml, and shake to elute virus particles;

(5) Use DMEM medium (containing 2% double antibody and 16μg/ml trypsin) to perform a 10-fold gradient dilution of the eluate, and the dilutions are respectively original times, 10 ^-1 , 10 ^-2 , ... 10 ^-6 A total of 7 dilutions;

(6) Discard the cell culture medium in the 96-well culture plate, add the diluted virus eluate, 100μ1 per well, 8 replicate wells per concentration, and then add 100μ1 DMEM medium (2% double antibody and 16μg/well) to each well. ml trypsin);

(7) Establish cell control and virus control at the same time; cell control: each well of 200μ1 DMEM medium (containing 2% double antibody and 16μg/ml trypsin) was added to the single layer of Vero E6 cell 96-well culture plate, a total of 4 Multiple holes. Virus control: each well of 100μ1 DMEM medium (containing 2% double antibody and 16μg/ml trypsin), then add an equal volume of 100TCID ₅₀ virus, a total of 4 multiple wells, 37 ℃ for 1h, after 1h discarded in the 96-well culture plate Cell culture medium, add the above-mentioned virus liquid.

(8) Incubate in a 37°C 5% CO ₂ incubator for 3 days;

(9) Observe cytopathic (CPE) under an optical microscope. Cells with CPE changes are recorded as "+", cells without CPE changes or normal cell morphology are recorded as "-".

Anti-virus effect calculation:

1) Calculation of reduction value M:

M=lg(Va/Vb);

2) Calculation of antiviral activity value Mv:

Mv=lg(Vb/Vc);

3) Calculation of killing rate:

Killing rate = (Vb-Vc)/Vb×100%;

The test results are shown in Table 4 and Table 5:

Table 4 1h antiviral activity and killing rate of materials

Table 5 Antiviral activity and killing rate of materials for 18h

The test results show:

The test material has a killing rate of >99% against the new coronavirus SARS-CoV-2 after acting for 1 hour; and the killing rate against the new coronavirus SARS-CoV-2 virus is over 99% after acting for 18 hours.

Example 5

This embodiment provides a method for detecting the filtration efficiency of NaCl particulate matter tested in accordance with the requirements of 6.3 of the national standard GB2626-2006. The detection object is a mask made by using the air purification component with the function of killing the coronavirus provided in Example 1. , The main technical parameters are as follows:

(1) The concentration of NaCl particles is not more than 200mg/m ³ , the median diameter (CMD) is (0.075±0.020) μm, and the geometric standard deviation of the particle size distribution is not more than 1.86;

(2) The dynamic range of the particle toughness detector is (0.001～200) mg/m ³ , and the accuracy is 1%;

(3) The detection flow range is (30～100) L/min, and the accuracy is 2%;

(4) The detection range of filtration efficiency is 0～99.999%;

(5) There should be a device capable of neutralizing the charge of the generated particulate matter.

The test results show that the filtering efficiency of the mask provided in this embodiment for salty particulate matter is ≥95%.

In summary, the present invention tests the air purification module with the function of killing coronavirus, and the result shows that its ^{filtration efficiency for sodium chloride particles with a size of 0.075 μm under the condition of a concentration of 200 mg/m 3} air flow is 95%; In addition, it was incubated with the two types of coronaviruses for 1 hour, and the results showed that the air purification component with the function of killing the coronaviruses had the killing rate of the two types of coronaviruses (GX_P2V and SARS-CoV-2) greater than 99%; For comparison, the polyethylene terephthalate composite copper fiber cloth in the air purification component with the function of killing the coronavirus is replaced with a pure polyethylene terephthalate fiber cloth without copper. , And incubated it with the two types of coronaviruses for 1 hour, and the results showed that there was no obvious killing effect on the coronavirus.

Obviously, the above-mentioned embodiments of the present invention are merely examples to clearly illustrate the present invention, and are not intended to limit the implementation of the present invention. For those of ordinary skill in the art, they can also do on the basis of the above description. In addition to other different forms of changes or changes, it is not possible to list all the implementations here. Any obvious changes or changes derived from the technical solutions of the present invention are still within the protection scope of the present invention.

Claims

An air purification component with the function of killing coronavirus, which is characterized by comprising a first supporting layer, a second supporting layer, and a functional material layer arranged between the first supporting layer and the second supporting layer; wherein the The functional material layer includes at least one organic-inorganic composite copper fiber material layer and at least one particulate filter layer.
The air purification component with the function of killing coronavirus according to claim 1, wherein the particulate filter layer is provided between the organic-inorganic composite copper fiber material layer and the first support layer, or is provided Between the organic-inorganic composite copper fiber material layer and the second support layer.
The air purification component with the function of killing coronavirus according to claim 1, wherein the functional material layer comprises multiple organic-inorganic composite copper fiber material layers and at least one particulate filter layer.
The air purification component with the function of killing coronavirus according to claim 3, wherein the particulate filter layer is arranged between multiple layers of organic-inorganic composite copper fiber material.
The air purification component with the function of killing the coronavirus according to claim 1, wherein the functional material layer comprises two layers of organic-inorganic composite copper fiber material and a layer located between the two organic-inorganic composite copper fiber material layers. A particulate filter layer between.
The air purification component with the function of killing coronavirus according to claim 1, wherein the average fiber diameter of the organic-inorganic composite copper fiber material in the organic-inorganic composite copper fiber material layer is 0.01-20 μm.
The air purification component with the function of killing coronavirus according to claim 1 or 6, wherein the mass fraction of copper in the organic-inorganic composite copper fiber material is 0.01-10%.
The air purification component with the function of killing coronavirus according to claim 1 or 7, wherein the organic-inorganic composite copper fiber material is a composite fiber material made by a composite method of polymer and copper; wherein The composite method includes one or more of nano master batch method, melt blending method, solution blending method, spray evaporation method, surface finishing method and graft modification method.
The air purification component with the function of killing coronavirus according to claim 1 or 8, wherein the material of the organic-inorganic composite copper fiber material layer comprises polyethylene terephthalate composite copper fiber cloth, One or more of polypropylene composite copper fiber cloth, polyurethane composite copper fiber cloth, polytetrafluoroethylene composite copper fiber cloth and cotton composite copper fiber cloth.
The air purification module with the function of killing coronavirus according to claim 1, wherein the material of the particulate filter layer includes polypropylene meltblown non-woven fabric, polyurethane nanofiber membrane, and polytetrafluoroethylene nanofiber membrane One or more of polyvinylidene fluoride nanofiber membranes, polypropylene nanofiber membranes and polyethylene terephthalate nanofiber membranes.
The air purification assembly with the function of killing coronavirus according to claim 1, wherein the materials of the first support layer and the second support layer respectively comprise polypropylene fiber cloth, cotton fiber cloth, and polyurethane fiber One or more of cloth, polyvinylidene fluoride fiber cloth and polytetrafluoroethylene fiber cloth.
An application of the air purification component with the function of killing coronaviruses according to any one of claims 1 to 11 in the preparation of an air purification device.
The application according to claim 12, wherein the air purification device comprises a mask, an air purifier or an air conditioner.
An air purification device comprising the air purification assembly with the function of killing coronaviruses according to any one of claims 1-11.