WO2021243170A8 - Procédé et système d'isolement d'arn à partir d'échantillons auto-collectés et de petit volume - Google Patents

Procédé et système d'isolement d'arn à partir d'échantillons auto-collectés et de petit volume Download PDF

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Publication number
WO2021243170A8
WO2021243170A8 PCT/US2021/034785 US2021034785W WO2021243170A8 WO 2021243170 A8 WO2021243170 A8 WO 2021243170A8 US 2021034785 W US2021034785 W US 2021034785W WO 2021243170 A8 WO2021243170 A8 WO 2021243170A8
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WO
WIPO (PCT)
Prior art keywords
self
small volume
collected
rna
rna isolation
Prior art date
Application number
PCT/US2021/034785
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English (en)
Other versions
WO2021243170A3 (fr
WO2021243170A2 (fr
Inventor
Robert B. Darnell
Dana ORANGE
Original Assignee
The Rockefeller University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Rockefeller University filed Critical The Rockefeller University
Priority to IL298468A priority Critical patent/IL298468A/en
Priority to CN202180058308.XA priority patent/CN116529371A/zh
Priority to JP2022573608A priority patent/JP2023527562A/ja
Priority to US17/928,424 priority patent/US20230203586A1/en
Priority to AU2021278969A priority patent/AU2021278969A1/en
Priority to EP21812697.7A priority patent/EP4158056A2/fr
Priority to CA3180092A priority patent/CA3180092A1/fr
Priority to KR1020227045593A priority patent/KR20230018435A/ko
Publication of WO2021243170A2 publication Critical patent/WO2021243170A2/fr
Publication of WO2021243170A3 publication Critical patent/WO2021243170A3/fr
Publication of WO2021243170A8 publication Critical patent/WO2021243170A8/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/537—Protease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)

Abstract

La présente invention concerne des procédés d'isolement et de caractérisation d'acide nucléique, en particulier d'ARN, à partir d'échantillons auto-collectés et de petit volume, incluant des échantillons de sang prélevé au bout du doigt, des écouvillons et des échantillons de salive. L'ARN dérivé est intact, de qualité suffisante et en quantité suffisante pour l'analyse de l'ARN, le séquençage d'ARN longitudinal et le typage transcriptomique global.
PCT/US2021/034785 2020-05-29 2021-05-28 Procédé et système d'isolement d'arn à partir d'échantillons auto-collectés et de petit volume WO2021243170A2 (fr)

Priority Applications (8)

Application Number Priority Date Filing Date Title
IL298468A IL298468A (en) 2020-05-29 2021-05-28 A method and system for isolating RNA from self-collected samples in a small volume
CN202180058308.XA CN116529371A (zh) 2020-05-29 2021-05-28 从自收集小体积样品中分离rna的方法和系统
JP2022573608A JP2023527562A (ja) 2020-05-29 2021-05-28 自己採取した小容量サンプルからｒｎａを単離するための方法及びシステム
US17/928,424 US20230203586A1 (en) 2020-05-29 2021-05-28 Method and system for rna isolation from self-collected and small volume samples
AU2021278969A AU2021278969A1 (en) 2020-05-29 2021-05-28 Method and system for RNA isolation from self-collected and small volume samples
EP21812697.7A EP4158056A2 (fr) 2020-05-29 2021-05-28 Procédé et système d'isolement d'arn à partir d'échantillons auto-collectés et de petit volume
CA3180092A CA3180092A1 (fr) 2020-05-29 2021-05-28 Procede et systeme d'isolement d'arn a partir d'echantillons auto-collectes et de petit volume
KR1020227045593A KR20230018435A (ko) 2020-05-29 2021-05-28 자기-수집된 및 작은 부피 샘플로부터의 rna 단리를 위한 방법 및 시스템

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
US202063031861P 2020-05-29 2020-05-29
US63/031,861 2020-05-29
US202063050155P 2020-07-10 2020-07-10
US63/050,155 2020-07-10
US202163135159P 2021-01-08 2021-01-08
US202163135224P 2021-01-08 2021-01-08
US63/135,159 2021-01-08
US63/135,224 2021-01-08
US202163171749P 2021-04-07 2021-04-07
US63/171,749 2021-04-07

Publications (3)

Publication Number Publication Date
WO2021243170A2 WO2021243170A2 (fr) 2021-12-02
WO2021243170A3 WO2021243170A3 (fr) 2021-12-30
WO2021243170A8 true WO2021243170A8 (fr) 2022-02-24

Family

ID=80496090

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2021/034785 WO2021243170A2 (fr) 2020-05-29 2021-05-28 Procédé et système d'isolement d'arn à partir d'échantillons auto-collectés et de petit volume

Country Status (8)

Country Link
US (1) US20230203586A1 (fr)
EP (1) EP4158056A2 (fr)
JP (1) JP2023527562A (fr)
KR (1) KR20230018435A (fr)
AU (1) AU2021278969A1 (fr)
CA (1) CA3180092A1 (fr)
IL (1) IL298468A (fr)
WO (1) WO2021243170A2 (fr)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2471924A1 (fr) * 2004-05-28 2012-07-04 Asuragen, INC. Procédés et compositions impliquant du microARN
US20160186262A1 (en) * 2013-01-23 2016-06-30 Reproductive Genetics And Technology Solutions, Llc Compositions and methods for genetic analysis of embryos
SG11201802946WA (en) * 2015-11-02 2018-05-30 Biofire Diagnostics Llc Sample preparation for difficult sample types

Also Published As

Publication number Publication date
JP2023527562A (ja) 2023-06-29
AU2021278969A1 (en) 2023-01-05
CA3180092A1 (fr) 2021-12-02
WO2021243170A3 (fr) 2021-12-30
EP4158056A2 (fr) 2023-04-05
IL298468A (en) 2023-01-01
US20230203586A1 (en) 2023-06-29
KR20230018435A (ko) 2023-02-07
WO2021243170A2 (fr) 2021-12-02

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