WO2021235158A1 - 蛍光指紋画像取得装置 - Google Patents

蛍光指紋画像取得装置 Download PDF

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Publication number
WO2021235158A1
WO2021235158A1 PCT/JP2021/016112 JP2021016112W WO2021235158A1 WO 2021235158 A1 WO2021235158 A1 WO 2021235158A1 JP 2021016112 W JP2021016112 W JP 2021016112W WO 2021235158 A1 WO2021235158 A1 WO 2021235158A1
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Prior art keywords
excitation light
wavelength
fluorescence
light
fingerprint image
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PCT/JP2021/016112
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English (en)
French (fr)
Japanese (ja)
Inventor
雄樹 小野
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コニカミノルタ株式会社
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Priority to CN202180035646.1A priority Critical patent/CN115667888A/zh
Priority to JP2022524339A priority patent/JPWO2021235158A1/ja
Publication of WO2021235158A1 publication Critical patent/WO2021235158A1/ja

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence

Definitions

  • the present invention relates to a fluorescence fingerprint image acquisition device, and in particular, when acquiring a fluorescence fingerprint image based on a fluorescence fingerprint (fluorescence characteristic) of an object, the fluorescence spectrum data can be compressed to increase the speed, and the apparatus can be used.
  • the present invention relates to a fluorescent fingerprint image acquisition device that can be simplified in configuration and can be realized at low cost.
  • the conventional spectroscopic fluorometer uses a xenon lamp as the excitation light source, selects a specific wavelength by spectroscopy, and irradiates the target.
  • a xenon lamp as the excitation light source
  • selects a specific wavelength by spectroscopy and irradiates the target.
  • Increasing the resolution of wavelength selection has a problem that the amount of excitation light that selectively uses only a part of the emitted wavelengths decreases. There is a trade-off between the resolution of wavelength selection and the amount of light.
  • Patent Document 1 when the fluorescence spectrum data of the object is acquired as two-dimensional data by photographing with a camera, the fluorescence spectrum data per measurement becomes enormous, so that the production process or the like There is a problem that quality monitoring cannot be performed in real time. Moreover, it is not suitable as a configuration for continuous measurement at high speed.
  • the present invention has been made in view of the above problems and situations, and the problem is that when acquiring a fluorescence fingerprint image based on the fluorescence fingerprint of an object, the fluorescence spectrum data can be compressed and speeded up. Moreover, it is to provide a fluorescent fingerprint image acquisition device which can simplify the configuration of the device and realize low cost.
  • the present inventor uses a one-dimensional line sensor as a measuring unit in the process of examining the cause of the above problems, thereby compressing the fluorescence spectrum data to enable high speed and speeding up.
  • a fluorescent fingerprint image acquisition device that can reduce the cost with a device having a simple configuration, and have arrived at the present invention. That is, the above-mentioned problem according to the present invention is solved by the following means.
  • a fluorescent fingerprint image acquisition device based on the measurement of the fluorescent fingerprint of an object.
  • the object is irradiated with an excitation light source that emits light having a specific excitation light wavelength.
  • the fluorescence from the object excited by the light of the excitation light wavelength is received by a measuring unit having a one-dimensional line sensor, and the fluorescence is received.
  • a fluorescent fingerprint image acquisition device that continuously acquires a fluorescent fingerprint image by making the relative positions of the measuring unit and the object variable.
  • the excitation light wavelength, the fluorescence wavelength, and the fluorescence intensity are measured in advance as the fluorescence fingerprint of the object, and a plurality of excitation light wavelengths effective for the measurement are selected. From the excitation light source that emits the light of the plurality of selected excitation light wavelengths, the light of the plurality of selected excitation light wavelengths is sequentially emitted for each wavelength, and the object is irradiated with the light.
  • the fluorescent fingerprint image acquisition device according to item 1, wherein the measuring unit receives fluorescence from the object excited by light having each excitation light wavelength.
  • an optical filter that absorbs or reflects light having a wavelength shorter than that of the excitation light and cuts the light is provided.
  • the fluorescent fingerprint image acquisition device according to any one of items 1 to 3, wherein the optical filter is switched in synchronization with the wavelength switching of the excitation light source.
  • a plurality of the one-dimensional line sensors corresponding to the excitation light wavelength of the excitation light source are provided.
  • an optical filter that absorbs or reflects light having a wavelength shorter than the excitation light wavelength and cuts the light is provided.
  • the fluorescent fingerprint image acquisition device according to any one of items 1 to 4, wherein the one-dimensional line sensor receives light in synchronization with the switching of the excitation light source.
  • the fluorescence spectrum data can be compressed to increase the speed, and the configuration of the apparatus can be simplified, resulting in low cost.
  • a possible fluorescent fingerprint image acquisition device can be provided.
  • the present invention is based on the fact that a one-dimensional line sensor receives light emitted by sequentially irradiating an object with light having a specific excitation light wavelength at a relative position, and continuously acquires a fluorescence fingerprint image. It is a characteristic feature. That is, in the present invention, since the one-dimensional line sensor is used instead of acquiring the spectrum data to be measured as two-dimensional data by camera photography or the like, continuous processing with high resolution is easy as compared with the area sensor. .. For example, in the case of sheet-shaped defect inspection, when inspecting continuously flowing objects, it is difficult to synchronize with the area sensor, whereas with the one-dimensional line sensor, it is output for each scan. Therefore, continuous processing becomes easy.
  • the object is sequentially irradiated with light having a specific excitation light wavelength, the fluorescence from the object is received by a measuring unit having a one-dimensional line sensor, and a fluorescent fingerprint image is continuously acquired.
  • a measuring unit having a one-dimensional line sensor it is possible to compress the fluorescence spectrum data to increase the speed, and it is possible to realize cost reduction with a device having a simple configuration.
  • FIG. 1 Flow chart showing the process of selecting multiple excitation light wavelengths that are effective for user measurement
  • Schematic diagram of the fluorescent fingerprint image acquisition device of the present invention A flowchart showing a control procedure by a processing unit executed by a fluorescent fingerprint image acquisition device.
  • the fluorescent fingerprint image acquisition device of the present invention is a fluorescent fingerprint image acquisition device based on the measurement of the fluorescent fingerprint of the object, and is a fluorescent fingerprint image acquisition device based on the measurement of the fluorescent fingerprint of the object.
  • Is irradiated from an excitation light source that emits light having a specific excitation light wavelength, and fluorescence from the object excited by the light having the excitation light wavelength is received by a measuring unit having a one-dimensional line sensor, and the measuring unit receives the fluorescence. It is characterized in that the relative position of the object is variable and fluorescent fingerprint images are continuously acquired. This feature is a technical feature common to or corresponding to each of the following embodiments.
  • the excitation light wavelength, the fluorescence wavelength, and the fluorescence intensity are measured in advance as the fluorescence fingerprint of the object, a plurality of excitation light wavelengths effective for the measurement are selected, and the plurality of selected excitation light wavelengths are selected. From the excitation light source that emits the light of the excitation light wavelength, the light of the plurality of selected excitation light wavelengths is sequentially emitted for each wavelength, the object is irradiated, and the light excited by the light of each of the excitation light wavelengths is used. It is preferable that the fluorescence from the object is received by the measuring unit. Therefore, first, in the measurement for acquiring the target fluorescent fingerprint image, it is not necessary to scan the entire wavelength range for the measurement, so that the fluorescent fingerprint information can be obtained by the measurement in a short time.
  • the excitation light source is a super-continue light source or an LED in terms of increasing the amount of light, eliminating the need for an optical filter switching mechanism in the light source unit, and simplifying and downsizing the device. ..
  • Stray light can be removed by providing an optical filter in front of the one-dimensional line sensor that absorbs or reflects and cuts light having a wavelength shorter than that of the excitation light, and switching the optical filter in synchronization with the wavelength switching of the excitation light source. It is preferable in terms of points.
  • An optic having a plurality of the one-dimensional line sensors corresponding to the excitation light wavelength of the excitation light source and absorbing or reflecting and cutting light having a wavelength shorter than the excitation light wavelength in front of each one-dimensional line sensor. It is preferable to provide a filter and receive light in synchronization with the switching of the excitation light source because the switching of the optical filter becomes unnecessary, the light receiving unit is simplified and downsized, and the measurement time is shortened. ..
  • the fluorescence fingerprint image acquisition device of the present invention is a fluorescence fingerprint image acquisition device based on the measurement of the fluorescence fingerprint of an object, and the object is irradiated with light of a specific excitation light wavelength from an excitation light source to emit the light.
  • the fluorescence from the object excited by the light of the excitation light wavelength is received by the measuring unit having a one-dimensional line sensor, the relative position between the measuring unit and the object is made variable, and the fluorescence fingerprint image is continuously obtained. get.
  • the excitation light wavelength, the fluorescence wavelength and the fluorescence intensity are measured in advance as the fluorescence fingerprint of the object, a plurality of excitation light wavelengths effective for the measurement are selected, and the plurality of selected excitation lights are selected. From an excitation light source that emits light of a wavelength, a plurality of selected lights of the excitation light wavelength are sequentially emitted for each wavelength, the object is irradiated with the light, and the object is excited by the light of each excitation light wavelength. It is preferable that the fluorescence from the above is received by the measuring unit.
  • Applications of the fluorescent fingerprint image acquisition device of the present invention include, for example, quality control of sheet-shaped products, detection of foreign substances, guarantee of producers of agricultural products, and authenticity determination of branded products (printed on tags using special paint). , Environmental inspection (water quality, soil, pesticides), agricultural use (disease judgment), etc.
  • Examples of the object include an object according to the above-mentioned use, and examples thereof include sheet-like products such as paper, film, and printed matter, agricultural products, branded products, and samples for environmental inspection and agriculture.
  • FIG. 1 is a flowchart showing a process of measuring a fluorescent fingerprint of an object in advance and selecting a plurality of excitation light wavelengths effective for the measurement.
  • the wavelength is changed in advance in a wide wavelength range, and the excitation light wavelength, the fluorescence wavelength, and the fluorescence intensity are measured as the fluorescence fingerprint (EEM) of the object (step S1).
  • EEM fluorescence fingerprint
  • a general spectrofluorescence fluorometer can be used, for example, a spectrofluorescence fluorometer FP-8000 (manufactured by Nippon Spectral Co., Ltd.) and a spectrofluorescence fluorometer F-7100 (Hitachi).
  • FIGS. 2A and 2B are graphs before and after heat is applied to a certain resin material, and a difference can be seen in the fluorescent fingerprints due to the heat applied.
  • the fluorescent fingerprint data is analyzed by principal component analysis (step S2).
  • the analysis is a multivariate analysis and is processed by a general personal computer.
  • a plurality of effective excitation light wavelengths used for determination are selected (step S3).
  • the contribution of the fluorescence wavelength can be obtained.
  • the fluorescence of a certain wavelength ⁇ 1 is detected, the substance A is included, and when the fluorescence of a certain wavelength ⁇ 2 is detected, the substance B is included.
  • the fluorescence spectrum becomes characteristic, and the result can be used to determine the inclusion of foreign matter. From the contribution of such fluorescence wavelengths, the user selects a plurality of desired excitation light wavelengths effective for measurement.
  • a fluorescent fingerprint image is acquired using the fluorescent fingerprint image acquisition device shown in FIG. 1
  • FIG. 3 is a schematic diagram of the fluorescent fingerprint image acquisition device of the present invention.
  • the fluorescent fingerprint image acquisition device 100 of the present invention is a device that acquires a fluorescent fingerprint image based on the fluorescent fingerprint of the object 200, and is at least an excitation light source 101, a measuring unit 102, an optical filter 103, and a carrier. It includes a mechanism 104 and a processing unit 105.
  • the excitation light source irradiates the object with light having a plurality of excitation light wavelengths selected in advance in the wavelength selection step to generate fluorescence from the components of the object.
  • it is a super-continue light source (a wideband pulse light source that emits strong light with a uniform phase over a very wide wavelength range by utilizing the non-linear effect of an optical fiber, and is also called an "SC light source") or an LED light source. And so on.
  • SC light source wideband pulse light source that emits strong light with a uniform phase over a very wide wavelength range by utilizing the non-linear effect of an optical fiber, and is also called an "SC light source”
  • LED light source LED light source.
  • the specific range of the excitation light wavelength is preferably within the range of visible light.
  • the wavelength of the excitation light emitted from the excitation light source can be arbitrarily changed by inputting a plurality of excitation light wavelengths effective for the measurement selected in advance by the user using the keyboard, mouse, or the like of the processing unit. It is possible.
  • the transport mechanism may have a mechanism capable of transporting an object, for example, an endless belt may be moved around by a roller, a measured object may be directly moved by a roller, or a roller may be used. May also move the object to be measured along the surface of a large cylindrical rotating drum.
  • the measuring unit receives the fluorescence of the pattern peculiar to the component emitted from the object by being irradiated with the excitation light, measures the excitation light wavelength, the fluorescence wavelength and the fluorescence intensity from the received fluorescence, and measures the fluorescence fingerprint data. Is sent to the processing unit.
  • the measuring unit includes a one-dimensional line sensor and an optical filter provided in front of the one-dimensional line sensor to absorb or reflect and cut light having a wavelength shorter than that of the excitation light.
  • a plurality of image pickup devices are arranged over the width of an object. Then, by moving the object in the transport direction, the one-dimensional line sensor can take a two-dimensional image on the object.
  • the one-dimensional line sensor examples include a CCD sensor (Charge Coupled Device) and a CMOS sensor (Complementary Metal Oxide Semiconductor).
  • the one-dimensional line sensor performs an image pickup operation in each image pickup device, which outputs a charge amount and a voltage according to the amount of fluorescence light input to the light receiving element from the surface of the object via an optical system (lens).
  • the one-dimensional line sensor is capable of imaging in a plurality of wavelength bands, and transmits the captured fluorescent fingerprint data to the processing unit.
  • the optical filter is controlled to switch to a desired optical filter that absorbs or reflects and cuts light having a wavelength shorter than the excitation light wavelength emitted from the excitation light source in synchronization with the wavelength switching of the excitation light source.
  • the optical filter include a cutoff filter, a bandpass filter (BPF, an interference filter), an acoustic optical wavelength variable filter (AOTF), a liquid crystal tunable filter and the like.
  • BPF bandpass filter
  • AOTF acoustic optical wavelength variable filter
  • a plurality of the one-dimensional line sensors may be provided corresponding to the excitation light wavelength of the excitation light source, and an optical filter may also be provided in front of the one-dimensional line sensor.
  • the processing unit is a device that acquires a fluorescent fingerprint image by processing abnormality detection and accompanying warnings and occurrence records from the fluorescent fingerprint data acquired by the measurement unit, and also performs the entire operation of the fluorescent fingerprint image acquisition device. Is centrally controlled.
  • a processing unit for example, a general personal computer or the like is used.
  • a dedicated integrated circuit ASIC: ASIC
  • the processing unit includes a memory, a control unit, a calculation processing unit, and the like.
  • the user can input measurement processing conditions and the like into the processing unit using the keyboard and mouse.
  • FIG. 4 is a flowchart showing a control procedure by the processing unit executed by the fluorescent fingerprint image acquisition device.
  • the excitation light source is switched to irradiate the first excitation light wavelength (step S11).
  • the optical filter of the measuring unit is also switched to an optical filter that cuts light having a wavelength shorter than the wavelength of the excitation light emitted from the excitation light source (step S12).
  • the object is irradiated with the excitation light from the switched excitation light source (step S13).
  • the irradiation time, irradiation intensity, etc. of the excitation light are appropriately set according to the object. Further, at this time, it is preferable that the object is transported so as to be irradiated at a desired position by the transport mechanism.
  • the object is irradiated with excitation light, the fluorescence from the object excited by the light having the excitation light wavelength is received by the measuring unit (step S14), and the wavelength and intensity of the received fluorescence are measured.
  • the fluorescence fingerprint data consisting of the fluorescence wavelength, the fluorescence intensity and the excitation light wavelength is transmitted to the processing unit.
  • step S15 If the irradiation of the set plurality of excitation light wavelengths is not completed (step S15; No), the light source is switched to the excitation light source that emits the next excitation light wavelength among the selected plurality of excitation light wavelengths (step S11). For example, when a plurality of selected excitation light wavelengths are two wavelengths of ⁇ 1 and ⁇ 2, a cycle of first irradiating with an excitation light source having a wavelength of ⁇ 1 and then irradiating with an excitation light source having a wavelength of ⁇ 2 is performed.
  • step S15 when all the irradiation of the set plurality of excitation light wavelengths is completed (step S15; Yes), the following determination process is performed based on the fluorescent fingerprint image created from the fluorescent fingerprint data transmitted to the processing unit, and the determination process is performed. Record the result (step S16).
  • the determination process includes, for example, the patterns of the determination process shown in the following (1) to (3), but the determination process is not limited thereto.
  • (1) It is determined whether or not a specific fluorescence wavelength is detected when a certain excitation light wavelength is irradiated. This is a case where the fluorescence characteristics peculiar to the substance to be detected are known in advance by a pattern simply determined by the presence or absence of a specific fluorescence wavelength.
  • (2) When a certain excitation light wavelength is irradiated, a specific fluorescence wavelength is detected, and it is determined whether or not the amount of light is equal to or greater than the threshold value.
  • step S17; No After performing the determination process as described above, if no abnormality is detected (step S17; No), the process ends.
  • an abnormality is detected (step S17; Yes)
  • a warning is issued by display or voice (step S18)
  • an abnormality occurrence record such as the place and time when the abnormality occurred and what kind of abnormality is taken is taken (step S18).
  • Step S19 the process is terminated.
  • the abnormality include cases where the fluorescence intensity ratio exceeds the set range, or fluorescence is generated from a place where fluorescence is not originally generated.
  • the fluorescent fingerprint of the object is measured in advance, a plurality of excitation light wavelengths effective for the measurement are selected from the measurement results, and the light is selected from the excitation light sources that emit light of the selected plurality of excitation light wavelengths. It was said that light with multiple excitation light wavelengths would be emitted sequentially for each wavelength and received by a one-dimensional line sensor to continuously acquire fluorescent fingerprint images, but effective excitation by measuring the fluorescent fingerprint of the object in advance. Without performing the step of selecting the light wavelength, the light of a specific excitation light wavelength is simply emitted to the object, the fluorescence from the excited object is received by the one-dimensional line sensor, and the fluorescence fingerprint is continuously fluorescent.
  • a transport mechanism for transporting the object is provided and the object is moved with respect to the measurement unit.
  • a transport mechanism for transporting the measurement unit is provided and the measurement unit is provided. May be configured to move with respect to the object.
  • the fluorescent fingerprint image acquisition device of the present embodiment when acquiring a fluorescent fingerprint image based on the fluorescent fingerprint of the object, a plurality of effective wavelengths related to the quality of the object are selected in advance. Moreover, by using a one-dimensional line sensor as a measuring unit, it is possible to compress spectral data to increase the speed, and it is possible to reduce the cost with a device having a simple configuration.
  • a resin molded product A to which an ultraviolet absorber was added and a resin molded product B to which an ultraviolet absorber was not added were prepared. Then, for each of these resin molded products A and B, the fluorescent fingerprints before and after heating were measured using the fluorescent fingerprint image acquisition device of the present invention, and the results of principal component analysis of the fluorescent fingerprint data are shown in FIG.
  • the excitation light wavelength at the time of measurement was 250 to 700 nm in 10 nm increments. As shown in FIG. 5, it can be seen that the presence / absence of the ultraviolet absorber and the presence / absence of heating can be separated by the axes of the main component 2 and the main component 3.
  • the fluorescence spectrum data when acquiring a fluorescence fingerprint image based on a fluorescence fingerprint (fluorescence characteristic) of an object, the fluorescence spectrum data can be compressed to increase the speed, and the configuration of the apparatus can be simplified, resulting in low cost. It can be used in a feasible fluorescent fingerprint image acquisition device.

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  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
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  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
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PCT/JP2021/016112 2020-05-19 2021-04-21 蛍光指紋画像取得装置 WO2021235158A1 (ja)

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CN202180035646.1A CN115667888A (zh) 2020-05-19 2021-04-21 荧光指纹图像取得装置
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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05215688A (ja) * 1991-07-10 1993-08-24 Asahi Glass Co Ltd 透光性物体中の散乱体検出方法及びその装置
JP2013108855A (ja) * 2011-11-21 2013-06-06 National Agriculture & Food Research Organization 判別フィルタ設計方法、判別方法、判別フィルタセット、判別装置、および、プログラム
JP2015130864A (ja) * 2009-05-15 2015-07-23 ビオメリュー・インコーポレイテッド 試料内の微生物因子の迅速な同定および／または特徴付けのためのシステムおよび方法
JP2018136151A (ja) * 2017-02-20 2018-08-30 日清オイリオグループ株式会社 油脂組成物の判定方法及び油脂組成物の製造方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05215688A (ja) * 1991-07-10 1993-08-24 Asahi Glass Co Ltd 透光性物体中の散乱体検出方法及びその装置
JP2015130864A (ja) * 2009-05-15 2015-07-23 ビオメリュー・インコーポレイテッド 試料内の微生物因子の迅速な同定および／または特徴付けのためのシステムおよび方法
JP2013108855A (ja) * 2011-11-21 2013-06-06 National Agriculture & Food Research Organization 判別フィルタ設計方法、判別方法、判別フィルタセット、判別装置、および、プログラム
JP2018136151A (ja) * 2017-02-20 2018-08-30 日清オイリオグループ株式会社 油脂組成物の判定方法及び油脂組成物の製造方法

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