WO2021209108A1 - Cleaning composition with disinfectant properties - Google Patents

Cleaning composition with disinfectant properties Download PDF

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Publication number
WO2021209108A1
WO2021209108A1 PCT/DK2021/050108 DK2021050108W WO2021209108A1 WO 2021209108 A1 WO2021209108 A1 WO 2021209108A1 DK 2021050108 W DK2021050108 W DK 2021050108W WO 2021209108 A1 WO2021209108 A1 WO 2021209108A1
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Prior art keywords
composition
cfu
lactic acid
amount
test
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PCT/DK2021/050108
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French (fr)
Inventor
Casper ERFURT KÜCKEN AAEN
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Panzerglass A/S
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N55/00—Biocides, pest repellants or attractants, or plant growth regulators, containing organic compounds containing elements other than carbon, hydrogen, halogen, oxygen, nitrogen and sulfur
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids

Definitions

  • the present invention relates to a cleaning composition with disinfectant properties comprising low amounts of lactic acid and sodium dodecyl sulphate.
  • the present in vention also relates to a method of producing a cleaning composition with disinfectant properties comprising low amounts of lactic acid and sodium dodecyl sulphate, as well as the use of the composition as a cleaning and a disinfectant agent.
  • compositions are well known in the prior art. Common to the compositions previously known is that they very often comprise alcohol. The problem with alcohol is that it is a hazardous chemical, and when used as a cleaning and disinfectant agent it has the tendency to dry the skin of human hands when ap plied.
  • the present invention relates to composition
  • composition comprising 0.01 - 1.0% w/w lactic acid, 0.01 - 1.0% w/w sodium dodecyl sulphate and 98.0 - 99.98% w/w water.
  • the present invention relates to a method of producing a composition comprising lactic acid and sodium dodecyl sulphate comprising the steps of a. Dissolving lactic acid and sodium dodecyl sulphate in water to obtain a solution b. Mixing the solution obtained in step a) to a homogenous composition c. Adjusting the pH of the composition obtained in step b) with a base to pH 3.0-5.0 d. obtaining a composition wherein the amount of lactic acid is 0.01 - 1.0% w/w of the composition and the amount of sodium dodecyl sul phate is 0.01 - 1.0% w/w of the composition.
  • the present invention relates to a composition comprising lactic acid and sodium dodecyl sulphate for use as a cleaning composition.
  • the present invention relates to a composition comprising lactic acid and sodium dodecyl sulphate for use as a disinfectant.
  • composition comprising low amounts of lactic acid (2-Hydroxypropanoic acid, C 3 H 6 O 3 , CAS NO.: 79-33-4 (L-Lactic Acid) and/or CAS NO.: 50-21-5 (racemic mixture of L- and D- Lactic acid)) and sodium dodecyl sulphate (NaC ⁇ LLsSO ⁇ CAS NO.: 151-21-3) has a very effective cleaning effect while at the same time being a very potent disinfectant towards a plethora of microorganisms.
  • lactic acid (2-Hydroxypropanoic acid, C 3 H 6 O 3 , CAS NO.: 79-33-4 (L-Lactic Acid) and/or CAS NO.: 50-21-5 (racemic mixture of L- and D- Lactic acid)
  • sodium dodecyl sulphate NaC ⁇ LLsSO ⁇ CAS NO.: 151-21-3
  • SDS sodium dodecyl sulphate
  • SLS sodium lauryl sulphate
  • the composition of the present invention further comprises a base.
  • the base may be any base (a Lewis base and/or a Bronsted-Lowry base) known to the skilled person in the art for raising the pH of a solution. Suitable bases may be mono-, di-, or trivalent metal or alkali metal hydroxides. In a preferred embodiment of the present invention the base is sodium hydroxide, NaOH.
  • composition of the present invention may comprise salts of lactic acid, such as for example sodium lactate.
  • the composition is consisting of 0.01 - 1.0% w/w lactic acid, 0.01 - 1.0% w/w sodium dodecyl sulphate and 98.0 - 99.98% w/w water.
  • the composition comprises or is consisting of a higher amount of lactic acid, such as more than 1.0% w/w of the total composition, such as 1.2% w/w, such as 1.5% w/w, for example 1.7% w/w, such as 2.0% w/w of the total composition.
  • the composition of the present invention may be prepared by a method comprising the steps of a. Dissolving lactic acid and sodium dodecyl sulphate in water to obtain a solution b. Mixing the solution obtained in step a) to a homogenous composition c. Adjusting the pH of the composition obtained in step b) with a base to pH 3.0-5.0 d. obtaining a composition wherein the amount of lactic acid is 0.01 - 1.0% w/w of the composition and the amount of sodium dodecyl sul phate is 0.01 - 1.0% w/w of the composition.
  • composition of the present invention is useful for cleaning and disinfectant pur poses, especially cleaning and disinfecting of surfaces.
  • Surfaces that benefit from the cleaning and disinfectant properties of the present invention include, but are not lim- ited to glass surfaces, plastic surfaces, metal surfaces, composite surfaces, silicone surfaces and the like.
  • composition according to the invention is suitable for cleaning and disinfecting the surface of hand-held computing devices such as mobile phones, tablets, touch screens, keyboards, computer keyboards, and any form of computer input devices. Also cleaning and disinfecting the surface of tel evisions, watches and glasses is within the scope of the present invention.
  • composition according to the invention is suitable for cleaning the surfaces of mobile phones, tablets and touch screens.
  • composition of the present invention is at the same time a very potent disinfectant agent.
  • composition of the present invention is capable of reducing, eliminating and/or preventing the growth and/or spread of microorganisms belonging to the group of mi croorganisms consisting of Clostridium, Pseudomonas aeruginosa, Proteus, Aer- omonas, Flavobacterium, Xanthomonas, Acinetobacter, Entero-viruses, salmonella, Listeria, Sars virus, Mers virus, Coronavirus, E-coli, Staph aureus, As.niger, Pe.fimiculosum, Ch.globosum, Cl.cladosporioides, Au.pullulans, G.
  • mi croorganisms consisting of Clostridium, Pseudomonas aeruginosa, Proteus, Aer- omonas, Flavobacterium, Xanthomonas, Acinetobacter, Entero-viruses, salmonella, Listeria, Sars virus, Mers virus,
  • virens Pae.variotii, MRSA, S.Enteritidis (NBRC-3313), V.Parahaemolyticus (NBRC-12711), B.Sutilis (NBRC-3134), Streptococcus and Staphylococcus.
  • the composition according to the invention is a bactericide capable of reducing, eliminating, and/or preventing the growth and/or spread of the bacteria belonging to the group of Pseudomonas aeru ginosa ATCC 15442, DSM 939, Escherichia coli ATCC 10536, DSM 682, Staphylo coccus aureus ATCC 6538, DSM 799 and Enterococcus hirae ATCC 10541, DSM 3320.
  • composition of the present invention composition is able to reduce the number of viable microorganisms by a factor of more than 1 x 10 5 CFU, such as more than 1.5 x 10 5 CFU, for example more than 2 x 10 5 CFU, such as 3 x 10 5 CFU, and even as much as 5 x 10 5 CFU as compared to a reference solution not comprising the composition of the present invention.
  • Example 1 Efficacy Test of the composition of the present invention comprising L- Lactic acid (CAS NO.: 79-33-4)
  • the product When tested in accordance with the test method under simulated clean conditions and under the required test conditions, the product shall demonstrate > log 5 reductions in viable counts for the four bacteria test strains in order to fulfil the requirements for DS/EN 1276:2019 for a bactericidal effect.
  • DS/EN 1276:2019 is a quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas.
  • test suspension was mixed with the interfering substance and the composition of the present invention at the given concentration. Following the contact time of 5 min. a subsample was mixed with a neutralizer to stop the effect. Subsamples and dilution series were plated, incubated and evaluated for the number of colony-forming-units (cfu). Validation tests were performed to check the effect of the experimental conditions, the toxicity of the neutralizer and the efficacy of the neutral- izer.
  • Test temperature Room temperature (20 - 25°C)
  • compositions of the present invention were tested: Table 1.
  • the products were diluted to 80% during the test, so e.g. a 125% concentrated batch of the product was used to achieve the 100% concentration in the test.
  • composition of the present invention fulfilled the requirements for bactericidal activity under simulated clean conditions according to DS/EN 1276:2019. At 200% concentration the composition also fulfilled the requirement for bactericidal activity under simulated clean conditions according to DS/EN 1276:2019 while the 20% concentration met the requirements for a bactericidal activity towards P. aeru ginosa, S. aureus and E. hirae.
  • Example 1A
  • Test organism Pseudomonas aeruginosa, ATCC 15442, DSM 939
  • N is the number of cfu per ml in the test suspension.
  • Nv is the number of cfu per ml in the validation suspension.
  • A, B and C is the number of cfu per ml following the tests for the experimental con ditions (A), the neutralizer control (B) and the method validation (C).
  • Na is the number of cfu in the test mixture at the end of the contact time.
  • R is the ratio between the starting concentra- tion of cfu and the concentration of cfu following the exposure time. Only cfu counts between
  • Test organism Staphylococcus aureus, ATCC 6538, DSM 799
  • Log(FF) is the log-value of the dilution factor.
  • N is the number of cfu per ml in the test suspension.
  • NO is the number of cfu per ml in the test mixture at the beginning of the contact time.
  • NO N/l 0.
  • Nv is the number of cfu per ml in the validation suspension.
  • A, B and C is the number of cfu per ml following the tests for the experimental conditions (A), the neutralizer control (B) and the method validation (C).
  • Na is the number of cfu in the test mix ture at the end of the contact time.
  • R is the ratio between the starting concentration of cfu and the concentration of cfu following the exposure time. Only cfu counts between 14 and 330 were used for calculations. ⁇ 14 cfu/plate was used for calculations in case of the undiluted samples showing below 14 cfu/plate. WMC is the weighted mean count.
  • Test organism Staphylococcus aureus, ATCC 6538, DSM 799
  • Log(FF) is the log-value of the dilution factor.
  • N is the number of cfu per ml in the test suspension.
  • NO is the number of cfu per ml in the test mixture at the beginning of the con- tact time.
  • NO N/l 0.
  • Nv is the number of cfu per ml in the validation suspension.
  • Nv/10 is the number of cfu in the validation mixtures A, B and C at the beginning of the con tact time.
  • A, B and C is the number of cfu per ml following the tests for the experimental con ditions (A), the neutralizer control (B) and the method validation (C).
  • Na is the number of cfu in the test mixture at the end of the contact time.
  • R is the ratio between the starting concentra- tion of cfu and the concentration of cfu following the exposure time. Only cfu counts between
  • Test organism Enterococcus hirae, ATCC 10541, DSM 3320
  • Log(FF) is the log-value of the dilution factor.
  • N is the number of cfu per ml in the test suspension.
  • NO is the number of cfu per ml in the test mixture at the beginning of the con- tact time.
  • NO N/l 0.
  • Nv is the number of cfu per ml in the validation suspension.
  • Nv/10 is the number of cfu in the validation mixtures A, B and C at the beginning of the con tact time.
  • A, B and C is the number of cfu per ml following the tests for the experimental con ditions (A), the neutralizer control (B) and the method validation (C).
  • Na is the number of cfu in the test mixture at the end of the contact time.
  • R is the ratio between the starting concentra- tion of cfu and the concentration of cfu following the exposure time. Only cfu counts between
  • the product When tested in accordance with the test method under simulated clean conditions and under the required test conditions, the product shall demonstrate > log 5 reductions in viable counts for the four bacteria test strains in order to fulfil the requirements for DS/EN 1276:2019 for a bactericidal effect.
  • DS/EN 1276:2019 is a quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas.
  • test suspension was mixed with the interfering substance and the composition of the present invention at the given concentration. Following the contact time of 5 min. a subsample was mixed with a neutralizer to stop the effect. Subsamples and dilution series were plated, incubated and evaluated for the number of colony-forming-units (cfu). Validation tests were performed to check the effect of the experimental conditions, the toxicity of the neutralizer and the efficacy of the neutral izer.
  • Test organisms Pseudomonas aeruginosa ATCC 15442, DSM 939
  • Test temperature Room temperature (20 - 25°C) Incubation of bacteria: (37 ⁇ 1) °C for (48 ⁇ 2) hours
  • TSA Tryptone soya agar
  • Table 7 The products were diluted to 80% during the test, so e.g. a 125% concentrated batch of the product was used to achieve the 100% concentration in the test.
  • composition of the present invention fulfilled the requirements for bactericidal activity under simulated clean conditions according to DS/EN 1276:2019.
  • composition also fulfilled the requirement for bactericidal activity under simulated clean conditions according to DS/EN 1276:2019 while the 20% concentration met the requirements for a bactericidal activity towards P. aeru ginosa, S. aureus and E. hirae.
  • Test organism Pseudomonas aeruginosa, ATCC 15442, DSM 939
  • Log(FF) is the log-value of the dilution factor.
  • N is the number of cfu per ml in the test suspension.
  • NO is the number of cfu per ml in the test mixture at the beginning of the con tact time.
  • NO N/l 0.
  • Nv is the number of cfu per ml in the validation suspension.
  • A, B and C is the number of cfu per ml following the tests for the experimental con ditions (A), the toxicity of the neutralizer (B) and the efficacy of the neutralizer (C).
  • Na is the number of cfu in the test mixture at the end of the contact time.
  • R is the ratio between the starting concentration of cfu and the concentration of cfu following the exposure time. Only cfu counts between 14 and 330 were used for calculations. ⁇ 14 cfu/plate was used for calcu lations in case of the undiluted samples showing below 14 cfu/plate.
  • WMC is the weighted mean count.
  • Test organism Escherichia coli, ATCC 10536, DSM 682
  • Log(FF) is the log-value of the dilution factor.
  • N is the number of cfu per ml in the test suspension.
  • NO is the number of cfu per ml in the test mixture at the beginning of the con tact time.
  • NO N/l 0.
  • Nv is the number of cfu per ml in the validation suspension.
  • A, B and C is the number of cfu per ml following the tests for the experimental conditions (A), the toxicity of the neutralizer (B) and the efficacy of the neutralizer (C).
  • Na is the number of cfu in the test mixture at the end of the contact time.
  • R is the ratio between the starting concentra tion of cfu and the concentration of cfu following the exposure time. Only cfu counts between 14 and 330 were used for calculations. ⁇ 14 cfu/plate was used for calculations in case of the undiluted samples showing below 14 cfu/plate.
  • WMC is the weighted mean count.
  • Test organism Enterococcus hirae, ATCC 10541, DSM 3320
  • Log(FF) is the log-value of the dilution factor.
  • N is the number of cfu per ml in the test suspension.
  • NO is the number of cfu per ml in the test mixture at the beginning of the con- tact time.
  • NO N/l 0.
  • Nv is the number of cfu per ml in the validation suspension.
  • Nv/10 is the number of cfu in the validation mixtures A, B and C at the beginning of the con tact time.
  • A, B and C is the number of cfu per ml following the tests for the experimental con ditions (A), the toxicity of the neutralizer (B) and the efficacy of the neutralizer (C).
  • Na is the number of cfu in the test mixture at the end of the contact time.
  • R is the ratio between the starting concentration of cfu and the concentration of cfu following the exposure time. Only cfu counts between 14 and 330 were used for calculations. ⁇ 14 cfu/plate was used for calcu lations in case of the undiluted samples showing below 14 cfu/plate.
  • WMC is the weighted mean count.
  • Test organism Staphylococcus aureus, ATCC 6538, DSM 799
  • Log(FF) is the log-value of the dilution factor.
  • N is the number of cfu per ml in the test suspension.
  • NO is the number of cfu per ml in the test mixture at the beginning of the con- tact time.
  • NO N/l 0.
  • Nv is the number of cfu per ml in the validation suspension.
  • Nv/10 is the number of cfu in the validation mixtures A, B and C at the beginning of the con tact time.
  • A, B and C is the number of cfu per ml following the tests for the experimental con ditions (A), the toxicity of the neutralizer (B) and the efficacy of the neutralizer (C).
  • Na is the number of cfu in the test mixture at the end of the contact time.
  • R is the ratio between the starting concentration of cfu and the concentration of cfu following the exposure time. Only cfu counts between 14 and 330 were used for calculations. ⁇ 14 cfu/plate was used for calcu lations in case of the undiluted samples showing below 14 cfu/plate.
  • WMC is the weighted mean count.

Abstract

The present invention relates to a composition comprising low amounts of lactic acid and sodium dodecyl sulphate. The invention is useful for cleaning and disinfecting surfaces such as glass and plastic surfaces, in particular the surface of screens of computing devices such as hand-held mobile devices.

Description

Cleaning composition with disinfectant properties Field of the Invention
The present invention relates to a cleaning composition with disinfectant properties comprising low amounts of lactic acid and sodium dodecyl sulphate. The present in vention also relates to a method of producing a cleaning composition with disinfectant properties comprising low amounts of lactic acid and sodium dodecyl sulphate, as well as the use of the composition as a cleaning and a disinfectant agent.
Background of the Invention
Cleaning and disinfectant compositions are well known in the prior art. Common to the compositions previously known is that they very often comprise alcohol. The problem with alcohol is that it is a hazardous chemical, and when used as a cleaning and disinfectant agent it has the tendency to dry the skin of human hands when ap plied.
Object of the Invention
Therefore it is an object of the present invention to provide a cleaning composition with disinfectant properties that eliminates the problems of similar compositions know in the prior art. In particular it is an object of the present invention to provide a com position that is a very effective cleaning and disinfectant composition, while at the same time being both environmentally-friendly and non-hazardous towards animals, including human beings, and does not dry the skin when applied.
Description of the Invention
Hence, in a first aspect the present invention relates to composition comprising 0.01 - 1.0% w/w lactic acid, 0.01 - 1.0% w/w sodium dodecyl sulphate and 98.0 - 99.98% w/w water.
In another aspect the present invention relates to a method of producing a composition comprising lactic acid and sodium dodecyl sulphate comprising the steps of a. Dissolving lactic acid and sodium dodecyl sulphate in water to obtain a solution b. Mixing the solution obtained in step a) to a homogenous composition c. Adjusting the pH of the composition obtained in step b) with a base to pH 3.0-5.0 d. obtaining a composition wherein the amount of lactic acid is 0.01 - 1.0% w/w of the composition and the amount of sodium dodecyl sul phate is 0.01 - 1.0% w/w of the composition. In yet another aspect the present invention relates to a composition comprising lactic acid and sodium dodecyl sulphate for use as a cleaning composition.
In a further aspect the present invention relates to a composition comprising lactic acid and sodium dodecyl sulphate for use as a disinfectant.
Detailed Description of the Invention
The inventors of the present invention have surprisingly found that a composition comprising low amounts of lactic acid (2-Hydroxypropanoic acid, C3H6O3 , CAS NO.: 79-33-4 (L-Lactic Acid) and/or CAS NO.: 50-21-5 (racemic mixture of L- and D- Lactic acid)) and sodium dodecyl sulphate (NaC^LLsSO^ CAS NO.: 151-21-3) has a very effective cleaning effect while at the same time being a very potent disinfectant towards a plethora of microorganisms.
In the context of the present invention “sodium dodecyl sulphate” (SDS) is inter- changeable with “sodium dodecyl sulfate” and which also is known as “sodium lauryl sulphate” (SLS) interchangeable with “sodium lauryl sulfate” sometimes written “so dium lauril sulphate” or “sodium lauril sulfate”.
In fact the inventor of the present invention has found that only small amounts of lac- tic acid in combination with sodium dodecyl sulphate is needed to obtain a highly ef ficient cleaning composition while at the same time have disinfectant effect against bacteria and other microorganisms. The composition of the present invention further comprises a base. The base may be any base (a Lewis base and/or a Bronsted-Lowry base) known to the skilled person in the art for raising the pH of a solution. Suitable bases may be mono-, di-, or trivalent metal or alkali metal hydroxides. In a preferred embodiment of the present invention the base is sodium hydroxide, NaOH.
Further, the composition of the present invention may comprise salts of lactic acid, such as for example sodium lactate. In an embodiment of the present invention the composition is consisting of 0.01 - 1.0% w/w lactic acid, 0.01 - 1.0% w/w sodium dodecyl sulphate and 98.0 - 99.98% w/w water.
However, it is also within the scope of the present invention that the composition comprises or is consisting of a higher amount of lactic acid, such as more than 1.0% w/w of the total composition, such as 1.2% w/w, such as 1.5% w/w, for example 1.7% w/w, such as 2.0% w/w of the total composition.
The composition of the present invention may be prepared by a method comprising the steps of a. Dissolving lactic acid and sodium dodecyl sulphate in water to obtain a solution b. Mixing the solution obtained in step a) to a homogenous composition c. Adjusting the pH of the composition obtained in step b) with a base to pH 3.0-5.0 d. obtaining a composition wherein the amount of lactic acid is 0.01 - 1.0% w/w of the composition and the amount of sodium dodecyl sul phate is 0.01 - 1.0% w/w of the composition.
The composition of the present invention is useful for cleaning and disinfectant pur poses, especially cleaning and disinfecting of surfaces. Surfaces that benefit from the cleaning and disinfectant properties of the present invention include, but are not lim- ited to glass surfaces, plastic surfaces, metal surfaces, composite surfaces, silicone surfaces and the like.
In an embodiment of the present invention the composition according to the invention is suitable for cleaning and disinfecting the surface of hand-held computing devices such as mobile phones, tablets, touch screens, keyboards, computer keyboards, and any form of computer input devices. Also cleaning and disinfecting the surface of tel evisions, watches and glasses is within the scope of the present invention.
In a preferred embodiment of the present invention the composition according to the invention is suitable for cleaning the surfaces of mobile phones, tablets and touch screens.
Besides being an effective cleaning agent, the composition of the present invention is at the same time a very potent disinfectant agent.
The composition of the present invention is capable of reducing, eliminating and/or preventing the growth and/or spread of microorganisms belonging to the group of mi croorganisms consisting of Clostridium, Pseudomonas aeruginosa, Proteus, Aer- omonas, Flavobacterium, Xanthomonas, Acinetobacter, Entero-viruses, salmonella, Listeria, Sars virus, Mers virus, Coronavirus, E-coli, Staph aureus, As.niger, Pe.fimiculosum, Ch.globosum, Cl.cladosporioides, Au.pullulans, G. virens, Pae.variotii, MRSA, S.Enteritidis (NBRC-3313), V.Parahaemolyticus (NBRC-12711), B.Sutilis (NBRC-3134), Streptococcus and Staphylococcus.
In a certain embodiment of the present invention the composition according to the invention is a bactericide capable of reducing, eliminating, and/or preventing the growth and/or spread of the bacteria belonging to the group of Pseudomonas aeru ginosa ATCC 15442, DSM 939, Escherichia coli ATCC 10536, DSM 682, Staphylo coccus aureus ATCC 6538, DSM 799 and Enterococcus hirae ATCC 10541, DSM 3320.
The composition of the present invention composition is able to reduce the number of viable microorganisms by a factor of more than 1 x 105 CFU, such as more than 1.5 x 105 CFU, for example more than 2 x 105 CFU, such as 3 x 105 CFU, and even as much as 5 x 105 CFU as compared to a reference solution not comprising the composition of the present invention.
Examples
Example 1. Efficacy Test of the composition of the present invention comprising L- Lactic acid (CAS NO.: 79-33-4)
The efficacy of the composition of the present invention at three different concentra tions was tested according to DS/EN 1276:2019.
When tested in accordance with the test method under simulated clean conditions and under the required test conditions, the product shall demonstrate > log 5 reductions in viable counts for the four bacteria test strains in order to fulfil the requirements for DS/EN 1276:2019 for a bactericidal effect.
DS/EN 1276:2019 is a quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas.
Test procedure
For each test organism, the test suspension was mixed with the interfering substance and the composition of the present invention at the given concentration. Following the contact time of 5 min. a subsample was mixed with a neutralizer to stop the effect. Subsamples and dilution series were plated, incubated and evaluated for the number of colony-forming-units (cfu). Validation tests were performed to check the effect of the experimental conditions, the toxicity of the neutralizer and the efficacy of the neutral- izer.
Experimental conditions Test organisms: Pseudomonas aeruginosa ATCC 15442, DSM 939 Escherichia coli ATCC 10536, DSM 682 Staphylococcus aureus ATCC 6538, DSM 799 Enterococcus hirae ATCC 10541, DSM 3320
Contact time: 5 min. ± lOsec.
Test temperature: Room temperature (20 - 25°C)
Incub ation of b acteri a : (37 ± 1) °C for (48 ±2) hours Growth media for bacteria: Tryptone soya agar (TSA)
Interfering substances: 0.3 g/L bovine serum albumin
(simulated clean conditions) Neutralizer: Saponin 30 g/L
Lecithin 3 g/L Polysorbate 80 30 g/L Na-thio sulphate 5 g/L L-histidin 1 g/L Diluted in 0.25 mmol/L phosphate buffer
Composition Different concentrations of the composition of the present invention were tested:
Figure imgf000007_0001
Table 1. The products were diluted to 80% during the test, so e.g. a 125% concentrated batch of the product was used to achieve the 100% concentration in the test.
Overall results of the composition of the present invention (Examples 1A-1D show detailed individual results)
Log reduction
Figure imgf000008_0001
Table 2. Log reduction. Individual detailed results are disclosed in Examples 1A-1D.
Conclusion
The composition of the present invention fulfilled the requirements for bactericidal activity under simulated clean conditions according to DS/EN 1276:2019. At 200% concentration the composition also fulfilled the requirement for bactericidal activity under simulated clean conditions according to DS/EN 1276:2019 while the 20% concentration met the requirements for a bactericidal activity towards P. aeru ginosa, S. aureus and E. hirae. Example 1A
Test organism: Pseudomonas aeruginosa, ATCC 15442, DSM 939
Figure imgf000008_0002
Figure imgf000009_0001
Table 3 .Log(FF) is the log-value of the dilution factor. N is the number of cfu per ml in the test suspension. NO is the number of cfu per ml in the test mixture at the beginning of the con- tact time. NO = N/l 0. Nv is the number of cfu per ml in the validation suspension. NvO = Nv/10 is the number of cfu in the validation mixtures A, B and C at the beginning of the con tact time. A, B and C is the number of cfu per ml following the tests for the experimental con ditions (A), the neutralizer control (B) and the method validation (C). Na is the number of cfu in the test mixture at the end of the contact time. R is the ratio between the starting concentra- tion of cfu and the concentration of cfu following the exposure time. Only cfu counts between
14 and 330 were used for calculations. <14 cfu/plate was used for calculations in case of the undiluted samples showing below 14 cfu/plate. WMC is the weighted mean count.
Example IB
Test organism: Staphylococcus aureus, ATCC 6538, DSM 799
Figure imgf000010_0001
Figure imgf000010_0002
Table 4. Log(FF) is the log-value of the dilution factor. N is the number of cfu per ml in the test suspension. NO is the number of cfu per ml in the test mixture at the beginning of the contact time. NO = N/l 0. Nv is the number of cfu per ml in the validation suspension. NvO = Nv/10 is the number of cfu in the validation mixtures A, B and C at the beginning of the contact time. A, B and C is the number of cfu per ml following the tests for the experimental conditions (A), the neutralizer control (B) and the method validation (C). Na is the number of cfu in the test mix ture at the end of the contact time. R is the ratio between the starting concentration of cfu and the concentration of cfu following the exposure time. Only cfu counts between 14 and 330 were used for calculations. <14 cfu/plate was used for calculations in case of the undiluted samples showing below 14 cfu/plate. WMC is the weighted mean count.
Example 1C
Test organism: Staphylococcus aureus, ATCC 6538, DSM 799
Figure imgf000011_0001
Figure imgf000011_0002
Table 5. Log(FF) is the log-value of the dilution factor. N is the number of cfu per ml in the test suspension. NO is the number of cfu per ml in the test mixture at the beginning of the con- tact time. NO = N/l 0. Nv is the number of cfu per ml in the validation suspension. NvO =
Nv/10 is the number of cfu in the validation mixtures A, B and C at the beginning of the con tact time. A, B and C is the number of cfu per ml following the tests for the experimental con ditions (A), the neutralizer control (B) and the method validation (C). Na is the number of cfu in the test mixture at the end of the contact time. R is the ratio between the starting concentra- tion of cfu and the concentration of cfu following the exposure time. Only cfu counts between
14 and 330 were used for calculations. <14 cfu/plate was used for calculations in case of the undiluted samples showing below 14 cfu/plate. WMC is the weighted mean count.
*All the validations were above the min. count of 14 cfu/plate so they could still be evaluated. Example ID
Test organism: Enterococcus hirae, ATCC 10541, DSM 3320
Figure imgf000012_0001
Figure imgf000012_0002
Table 6. Log(FF) is the log-value of the dilution factor. N is the number of cfu per ml in the test suspension. NO is the number of cfu per ml in the test mixture at the beginning of the con- tact time. NO = N/l 0. Nv is the number of cfu per ml in the validation suspension. NvO =
Nv/10 is the number of cfu in the validation mixtures A, B and C at the beginning of the con tact time. A, B and C is the number of cfu per ml following the tests for the experimental con ditions (A), the neutralizer control (B) and the method validation (C). Na is the number of cfu in the test mixture at the end of the contact time. R is the ratio between the starting concentra- tion of cfu and the concentration of cfu following the exposure time. Only cfu counts between
14 and 330 were used for calculations. <14 cfu/plate was used for calculations in case of the undiluted samples showing below 14 cfu/plate. WMC is the weighted mean count. Example 2. Efficacy Test of the composition of the present invention comprising a racemic mixture of L-Lactic Acid and D- Lactic Acid (CAS NO. : 50-21-5)
The efficacy of the composition of the present invention at three different concentra tions was tested according to DS/EN 1276:2019.
When tested in accordance with the test method under simulated clean conditions and under the required test conditions, the product shall demonstrate > log 5 reductions in viable counts for the four bacteria test strains in order to fulfil the requirements for DS/EN 1276:2019 for a bactericidal effect.
DS/EN 1276:2019 is a quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas.
Test procedure
For each test organism, the test suspension was mixed with the interfering substance and the composition of the present invention at the given concentration. Following the contact time of 5 min. a subsample was mixed with a neutralizer to stop the effect. Subsamples and dilution series were plated, incubated and evaluated for the number of colony-forming-units (cfu). Validation tests were performed to check the effect of the experimental conditions, the toxicity of the neutralizer and the efficacy of the neutral izer.
Experimental conditions
Test organisms: Pseudomonas aeruginosa ATCC 15442, DSM 939
Escherichia coli ATCC 10536, DSM 682 Staphylococcus aureus ATCC 6538, DSM 799 Enterococcus hirae ATCC 10541, DSM 3320
Contact time: 5 min. ± lOsec.
Test temperature: Room temperature (20 - 25°C) Incubation of bacteria: (37 ± 1) °C for (48 ±2) hours
Growth media for bacteria: Tryptone soya agar (TSA) Interfering substances: 0.3 g/L bovine serum albumin
(simulated clean conditions)
Neutralizer: Saponin 30 g/L Lecithin 3 g/L Polysorbate 80 30 g/L Na-thio sulphate 5 g/L L-histidin 1 g/L
Diluted in 0.25 mmol/L phosphate buffer
Composition
Different concentrations of the composition of the present invention were tested:
Figure imgf000014_0001
Table 7 The products were diluted to 80% during the test, so e.g. a 125% concentrated batch of the product was used to achieve the 100% concentration in the test.
Overall results of the composition of the present invention (Examples 2A-2D show detailed individual results)
Log reduction
Figure imgf000015_0001
Table 8. Log reduction. Individual detailed results are disclosed in Examples 2A-2D.
Conclusion The composition of the present invention fulfilled the requirements for bactericidal activity under simulated clean conditions according to DS/EN 1276:2019.
At 200% concentration the composition also fulfilled the requirement for bactericidal activity under simulated clean conditions according to DS/EN 1276:2019 while the 20% concentration met the requirements for a bactericidal activity towards P. aeru ginosa, S. aureus and E. hirae.
Example 2 A
Test organism: Pseudomonas aeruginosa, ATCC 15442, DSM 939
Figure imgf000015_0002
Figure imgf000015_0003
Figure imgf000016_0001
Table 9. Log(FF) is the log-value of the dilution factor. N is the number of cfu per ml in the test suspension. NO is the number of cfu per ml in the test mixture at the beginning of the con tact time. NO = N/l 0. Nv is the number of cfu per ml in the validation suspension. NvO = Nv/10 is the number of cfu in the validation mixtures A, B and C at the beginning of the con tact time. A, B and C is the number of cfu per ml following the tests for the experimental con ditions (A), the toxicity of the neutralizer (B) and the efficacy of the neutralizer (C). Na is the number of cfu in the test mixture at the end of the contact time. R is the ratio between the starting concentration of cfu and the concentration of cfu following the exposure time. Only cfu counts between 14 and 330 were used for calculations. <14 cfu/plate was used for calcu lations in case of the undiluted samples showing below 14 cfu/plate. WMC is the weighted mean count.
Example 2B
Test organism: Escherichia coli, ATCC 10536, DSM 682
Figure imgf000017_0001
Figure imgf000017_0002
Table 10. Log(FF) is the log-value of the dilution factor. N is the number of cfu per ml in the test suspension. NO is the number of cfu per ml in the test mixture at the beginning of the con tact time. NO = N/l 0. Nv is the number of cfu per ml in the validation suspension. NvO = Nv/10 is the number of cfu in the validation mixtures A, B and C at the beginning of the contact time.
A, B and C is the number of cfu per ml following the tests for the experimental conditions (A), the toxicity of the neutralizer (B) and the efficacy of the neutralizer (C). Na is the number of cfu in the test mixture at the end of the contact time. R is the ratio between the starting concentra tion of cfu and the concentration of cfu following the exposure time. Only cfu counts between 14 and 330 were used for calculations. <14 cfu/plate was used for calculations in case of the undiluted samples showing below 14 cfu/plate. WMC is the weighted mean count.
Example 2C
Test organism: Enterococcus hirae, ATCC 10541, DSM 3320
Figure imgf000018_0001
Figure imgf000018_0002
Table 11. Log(FF) is the log-value of the dilution factor. N is the number of cfu per ml in the test suspension. NO is the number of cfu per ml in the test mixture at the beginning of the con- tact time. NO = N/l 0. Nv is the number of cfu per ml in the validation suspension. NvO =
Nv/10 is the number of cfu in the validation mixtures A, B and C at the beginning of the con tact time. A, B and C is the number of cfu per ml following the tests for the experimental con ditions (A), the toxicity of the neutralizer (B) and the efficacy of the neutralizer (C). Na is the number of cfu in the test mixture at the end of the contact time. R is the ratio between the starting concentration of cfu and the concentration of cfu following the exposure time. Only cfu counts between 14 and 330 were used for calculations. <14 cfu/plate was used for calcu lations in case of the undiluted samples showing below 14 cfu/plate. WMC is the weighted mean count. Example 2D
Test organism: Staphylococcus aureus, ATCC 6538, DSM 799
Figure imgf000019_0001
Figure imgf000019_0002
Table 12. Log(FF) is the log-value of the dilution factor. N is the number of cfu per ml in the test suspension. NO is the number of cfu per ml in the test mixture at the beginning of the con- tact time. NO = N/l 0. Nv is the number of cfu per ml in the validation suspension. NvO =
Nv/10 is the number of cfu in the validation mixtures A, B and C at the beginning of the con tact time. A, B and C is the number of cfu per ml following the tests for the experimental con ditions (A), the toxicity of the neutralizer (B) and the efficacy of the neutralizer (C). Na is the number of cfu in the test mixture at the end of the contact time. R is the ratio between the starting concentration of cfu and the concentration of cfu following the exposure time. Only cfu counts between 14 and 330 were used for calculations. <14 cfu/plate was used for calcu lations in case of the undiluted samples showing below 14 cfu/plate. WMC is the weighted mean count.

Claims

Claims
1. A composition comprising 0.01 - 1.0% w/w lactic acid, 0.01 - 1.0% w/w sodi um dodecyl sulphate and 98.0 - 99.98% w/w water.
2. The composition according to claim 1 wherein the amount of lactic acid is 0.02 - 0.80% w/w of the composition, such as 0.05 - 0.70% w/w, such as 0.1 - 0.6% w/w, such as 0.3 - 0.5% w/w of the composition.
3. The composition according to claim 1 or 2 wherein the amount of lactic acid is 0.4 % w/w of the composition.
4. The composition according to any one of the preceding claims wherein the amount of sodium dodecyl sulphate is 0.02 - 0.80% w/w of the composition, such as 0.05 - 0.75% w/w, such as 0.10 - 0.70% w/w, such as 0.30 - 0.60% w/w of the composition.
5. The composition according to any one of the preceding claims wherein the amount of sodium dodecyl sulphate is 0.5% w/w of the composition.
6. The composition according to any one of the preceding claims further com prising a base, preferably sodium hydroxide.
7. The composition according to any one of the preceding claims wherein the pH of the composition is 3.0 - 5.0, preferably approximately 4.0.
8. The composition according to any one of the preceding claims further com prising
9. The composition according to any one of the preceding claims wherein said composition is able to reduce the amount of viable microorganisms by a factor of more than 1 x 105 cfu as compared to a reference solution not comprising the composition of the present invention.
10. A method of producing a composition comprising lactic acid and sodium do- decyl sulphate comprising the steps of a. Dissolving lactic acid and sodium dodecyl sulphate in water to obtain a solution b. Mixing the solution obtained in step a) to a homogenous composition c. Adjusting the pH of the composition obtained in step b) with a base to pH 3.0-5.0 d. Obtaining a composition wherein the amount of lactic acid is 0.01 - 1.0% w/w of the composition and the amount of sodium dodecyl sul phate is 0.01 - 1.0% w/w of the composition.
11. The method according to claim 10 wherein the composition obtained in step d) comprises an amount of lactic acid of 0.4% w/w of the composition and an amount of sodium dodecyl sulphate of 0.5% w/w, and wherein the composi tion has a pH of approximately 4.0.
12. Use of a composition according to claims 1-9 as a cleaning agent.
13. Use of a composition according to claims 1-9 as a disinfectant agent.
14. The use according to claim 13 wherein said composition has disinfectant prop erties against microorganisms such as bacteria, viruses, and yeast.
15. The use according to claim 14 as disinfectant agent against the bacteria P. ae ruginosa, S. aureus, E. hirae and E. coli.
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