WO2021203539A1 - Biological antimicrobial agent and use thereof in keeping fresh cordyceps sinensis fresh - Google Patents

Biological antimicrobial agent and use thereof in keeping fresh cordyceps sinensis fresh Download PDF

Info

Publication number
WO2021203539A1
WO2021203539A1 PCT/CN2020/093021 CN2020093021W WO2021203539A1 WO 2021203539 A1 WO2021203539 A1 WO 2021203539A1 CN 2020093021 W CN2020093021 W CN 2020093021W WO 2021203539 A1 WO2021203539 A1 WO 2021203539A1
Authority
WO
WIPO (PCT)
Prior art keywords
polylysine
biological
bacteriostatic agent
natamycin
fresh
Prior art date
Application number
PCT/CN2020/093021
Other languages
French (fr)
Chinese (zh)
Inventor
李光荣
刘欢
张文祥
郑东城
梁关海
邱仕奇
汤玉兰
邱健健
李文佳
刘仁香
Original Assignee
东莞市东阳光冬虫夏草研发有限公司
宜昌山城水都冬虫夏草有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 东莞市东阳光冬虫夏草研发有限公司, 宜昌山城水都冬虫夏草有限公司 filed Critical 东莞市东阳光冬虫夏草研发有限公司
Publication of WO2021203539A1 publication Critical patent/WO2021203539A1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A23L3/3562—Sugars; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/26—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by irradiation without heating
    • A23L3/263—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by irradiation without heating with corpuscular or ionising radiation, i.e. X, alpha, beta or omega radiation
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A23L3/3526—Organic compounds containing nitrogen
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/36—Freezing; Subsequent thawing; Cooling
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof

Definitions

  • the invention relates to the field of preservation, in particular to a biological type bacteriostatic agent and its application in the preservation of fresh cordyceps.
  • Patent document CN110628633A discloses a method for preserving fresh Cordyceps sinensis and fresh Cordyceps sinensis products. It adopts a modified atmosphere packaging method and is filled with a gas ratio of 50-100% by volume of oxygen, 0-40% by volume of carbon dioxide and 0-50% by volume of nitrogen. Keep fresh under refrigeration at 0-10°C.
  • Patent document CN109717346A discloses a method of radiation preservation of Cordyceps sinensis, which is first soaked with a preservative, then packaged in a modified atmosphere, and finally sterilized by irradiation, and kept fresh under refrigerated conditions at 0-4°C. It can be seen that the prior art generally adopts modified atmosphere packaging or bacteriostatic agent combined with modified atmosphere packaging to keep fresh Cordyceps under refrigerated conditions for a longer period of time. However, the cost of modified atmosphere packaging equipment is relatively high and there are many operations, which is not conducive to Commercial promotion.
  • the first objective of the present invention is to overcome the defects of the prior art and provide a biological bacteriostatic agent that is healthy, safe, non-toxic, no peculiar smell, good bacteriostatic effect, and convenient to use.
  • the active ingredients of the biological bacteriostatic agent provided by the present invention include chitosan lactate, ⁇ -polylysine or ⁇ -polylysine salt, and natamycin.
  • the active ingredient of the biological bacteriostatic agent is composed of chitosan lactate, ⁇ -polylysine or ⁇ -polylysine salt, and natamycin.
  • the ⁇ -polylysine salt in the present invention refers to the organic or inorganic acid salt of ⁇ -polylysine, which is a salt formed by an acceptable non-toxic acid, including, but not limited to, an inorganic acid salt such as a salt Acid salts, hydrobromide, phosphate, sulfate, perchlorate, and organic acid salts such as acetate, lactate, oxalate, maleate, tartrate, citrate, succinic acid Salt, malonate, or other methods described in books and literature such as ion exchange to obtain these salts.
  • an inorganic acid salt such as a salt Acid salts, hydrobromide, phosphate, sulfate, perchlorate, and organic acid salts such as acetate, lactate, oxalate, maleate, tartrate, citrate, succinic acid Salt, malonate, or other methods described in books and literature such as ion exchange to obtain these salts.
  • the ⁇ -polylysine salt is ⁇ -polylysine hydrochloride, ⁇ -polylysine lactate or ⁇ -polylysine acetate.
  • the mass ratio of chitosan lactate, ⁇ -polylysine or ⁇ -polylysine salt and natamycin is (1-15 ): (0.1 ⁇ 2): (0.01 ⁇ 0.2).
  • the mass ratio of chitosan lactate, ⁇ -polylysine or ⁇ -polylysine salt and natamycin is (3-10 ): (0.1 ⁇ 1): (0.02 ⁇ 0.15).
  • the mass ratio of chitosan lactate, ⁇ -polylysine or ⁇ -polylysine salt and natamycin is 6:1: 0.1.
  • the biological antibacterial agent provided by the present invention may also contain conventional auxiliary materials in the art to form a specific dosage form.
  • the biological bacteriostatic agent is a solution, preferably an aqueous solution.
  • the solution of the biological bacteriostatic agent contains the following components in mass concentration: chitosan lactate 0.1-1.5%, ⁇ -polylysine or ⁇ -polylysine Salt 0.01 ⁇ 0.2%, Natamycin 0.002 ⁇ 0.02%.
  • the solution of the biological bacteriostatic agent contains the following components in mass concentration: chitosan lactate 0.3-1%, ⁇ -polylysine or ⁇ -polylysine Salt 0.01 ⁇ 0.1%, Natamycin 0.002 ⁇ 0.015%.
  • the biological bacteriostatic agent solution contains the following components in mass concentration: chitosan lactate 0.6%, ⁇ -polylysine or ⁇ -polylysine salt 0.1 %, natamycin 0.01%.
  • the solution of the biological antibacterial agent provided by the present invention may contain conventional excipients in other solvent formulations, or may not contain any other excipients.
  • the solution of the biological antibacterial agent is composed of the following components in mass concentration: chitosan lactate 0.1 to 1.5%, ⁇ -polylysine or ⁇ -polylysine salt 0.01 ⁇ 0.2%, natamycin 0.002 ⁇ 0.015%, the balance is solvent.
  • the solution of the biological antibacterial agent is composed of the following components in mass concentration: chitosan lactate 0.3-1%, ⁇ -polylysine or ⁇ -polylysine salt 0.01 ⁇ 0.1%, natamycin 0.002 ⁇ 0.015%, the balance is solvent.
  • the solution of the biological antibacterial agent is composed of the following components in mass concentration: chitosan lactate 0.6%, ⁇ -polylysine or ⁇ -polylysine 0.1%, Natamycin is 0.01%, the remainder is solvent.
  • the ⁇ -polylysine salt is ⁇ -polylysine hydrochloride.
  • the biological bacteriostatic agent is a bacteriostatic agent for preservation of fresh Cordyceps sinensis.
  • the fresh Cordyceps sinensis in the present invention refers to fresh Cordyceps sinensis that has not undergone drying, heating or other processing treatments.
  • the bio-type bacteriostatic agent provided by the invention is healthy, safe, non-toxic and has no peculiar smell.
  • the preparation method of the bacteriostatic agent is simple; when preparing a solution-type bacteriostatic agent, all components only need to be dissolved in water.
  • the biological bacteriostatic agent has excellent effects on the preservation of fresh Cordyceps sinensis.
  • the second object of the present invention is to provide the application of the biological antibacterial agent in the preservation of fresh Chinese medicinal materials.
  • the fresh Chinese medicinal material is fresh Cordyceps sinensis.
  • the preservation refers to preservation at low temperature, such as preservation at a temperature of 0-8°C.
  • the third object of the present invention is to provide a method for preserving fresh Cordyceps sinensis, the method comprising using the bio-type bacteriostatic agent provided by the present invention to treat the fresh Cordyceps sinensis.
  • the preservation method includes: first treating the fresh Cordyceps sinensis with the biological bacteriostatic agent, then sterilizing it, and then storing it at 0-8°C.
  • the preservation method includes: immersing the fresh cordyceps in the biological bacteriostatic agent solution, preferably soaking for 3-15 minutes, taking it out, draining, and sterilizing, Then store at 0-8°C.
  • the sterilization in the present invention is preferably irradiation sterilization, such as using 1.8 KGy electron beam irradiation sterilization.
  • Adopting the method provided by the present invention to keep fresh Cordyceps sinensis can ensure that Staphylococcus aureus, Salmonella, Escherichia coli, coliforms and total colony counts in fresh Cordyceps are in line with pathogenic bacteria in food when stored for 60 days
  • the limit standard of, the cold storage shelf life of fresh Cordyceps can reach more than 60 days.
  • the fresh-keeping method provided by the present invention has a simple processing process, does not require modified atmosphere packaging, has low cost, and is easy to popularize.
  • the fresh Cordyceps sinensis to be treated comes from Yidu Shancheng Shuidu Cordyceps Co., Ltd.; chitosan lactate, with a degree of deacetylation of over 85%, purchased from Qingdao Honghai Technology Co., Ltd.; ⁇ -polylysine salt Acetate and natamycin were purchased from Zhengzhou Binafo Bioengineering Co., Ltd.; tea polyphenols and sodium chloride were purchased from Zhejiang Yinuo Biotechnology Co., Ltd.; parabens were purchased from Zhejiang Shengxiao Chemical Co., Ltd. company.
  • the bacteriostatic agent is an aqueous solution, which is composed of components with the following concentrations: chitosan lactate 3g/kg, ⁇ -polylysine hydrochloride 0.1g/kg, and natamycin 0.02g/kg.
  • the bacteriostatic agent is an aqueous solution, which is composed of components with the following concentrations: chitosan lactate 10g/kg, ⁇ -polylysine hydrochloride 1g/kg, and natamycin 0.15g/kg.
  • the bacteriostatic agent is an aqueous solution, which consists of the following components at a concentration of 6 g/kg of chitosan lactate, 1 g/kg of ⁇ -polylysine hydrochloride, and 0.1 g/kg of natamycin.
  • This comparative example provides a biological type bacteriostatic agent. Compared with Example 3, the only difference is that chitosan hydrochloride is used instead of the chitosan lactate.
  • This comparative example provides a biological type bacteriostatic agent. Compared with Example 3, the only difference is that chitosan acetate is used instead of the chitosan lactate.
  • This comparative example provides a biological type bacteriostatic agent. Compared with Example 3, the only difference is that chitosan is used instead of the chitosan lactate.
  • the bacteriostatic agent is an aqueous solution, which is composed of the following components: chitosan 6g/kg, ⁇ -polylysine hydrochloride 1g/kg, natamycin 0.1g/kg, p-hydroxybenzyl Acid ester 0.05%.
  • the bacteriostatic agent is an aqueous solution, which is composed of the following components: chitosan 6g/kg, ⁇ -polylysine hydrochloride 1g/kg, natamycin 0.1g/kg, sodium chloride 1 %.
  • the bacteriostatic agent is an aqueous solution, which is composed of the following components: chitosan 6g/kg, ⁇ -polylysine hydrochloride 1g/kg, natamycin 0.1g/kg, acetic acid 0.2%, Tea polyphenols 0.03%, glucose oxidase 0.05%.
  • the biological bacteriostatic agent provided by this comparative example is a single-component aqueous solution, specifically:
  • Comparative Example 7-1 Chitosan hydrochloride with a concentration of 6g/kg
  • Comparative Example 7-2 Chitosan lactate with a concentration of 6g/kg
  • Comparative Example 7-3 Chitosan acetate with a concentration of 6g/kg
  • Comparative Example 7-4 ⁇ -polylysine hydrochloride with a concentration of 1g/kg
  • Comparative example 7-5 Natamycin with a concentration of 0.1g/kg
  • Comparative Example 7-6 Chitosan with a concentration of 6g/kg.
  • Example 2 Take the biological bacteriostatic agent solution provided in Example 1, put 120 cleaned fresh Cordyceps into it for 3 minutes, take it out and drain, put them into 120 glass tubes, and irradiate them with 1.8KGy electron beam , Placed in the refrigerator at 0 °C preservation. Take out 20 roots every 15 days to determine the microbial content. The results are shown in Table 2.
  • Example 3 Take the biological bacteriostatic agent solution provided in Example 3, put 120 cleaned fresh Cordyceps into it for 5 minutes, take it out and drain, put them into 120 glass tubes, and irradiate them with 1.8KGy electron beam , Placed in a refrigerator at 4°C for preservation. Take out 20 roots every 15 days to determine the microbial content.
  • Example 3 As a control, first 120 cleaned fresh Cordyceps were sterilized by 1.8KGy electron beam irradiation, and then put into the bio-type bacteriostatic agent solution provided in Example 3 (which had been sterilized) for 5 minutes, and then removed. Drain them, put them into 120 glass tubes, and store them in a refrigerator at 4°C. Take out 20 roots every 15 days to determine the microbial content.
  • Example 3 the washed fresh Cordyceps was sterilized by 1.8KGy electron beam irradiation, and then put into the sterilized biological bacteriostatic solution provided in Example 3 and Comparative Examples 1-7 (each type of bacteriostatic Put 120 pieces in each of the reagent solution) soak for 5min, take it out and drain, put them into glass tubes, and place them in a refrigerator at 4°C for storage. From the 21st day, 20 roots were taken from each bacteriostatic solution every 7 days to determine the microbial content. The results are shown in Table 5.
  • the sterilized fresh Cordyceps sinensis was treated with the bacteriostatic agent provided in Comparative Example 1, Comparative Example 2 and Comparative Example 3 and then stored under refrigeration.
  • the indicators began to be unqualified on the 35th day (Once unqualified, No further testing), the shelf life can only reach 28 days.
  • the sterilized fresh Cordyceps sinensis was treated with the bacteriostatic agent provided in Comparative Example 4, Comparative Example 5, and Comparative Example 6 and stored under refrigeration.
  • the indicators began to be unqualified on the 35th day (Once unqualified, No further testing), the shelf life can only reach 28 days.
  • the parabens in Comparative Example 4 are not safe enough as chemical preservatives.
  • the sodium chloride in Comparative Example 5 and the acetic acid in Comparative Example 6 will change the taste of fresh Cordyceps sinensis.
  • Comparative Example 7 In comparison, after sterilization of fresh Cordyceps sinensis treated by Comparative Example 7 (6 groups of bacteriostatic agents with a single active ingredient), the shelf life of cold storage is not longer than 28 days; Among them, Comparative Examples 7-6, that is, the concentration of 6g/kg The shelf life of chitosan aqueous solution can only reach 21 days in cold storage.
  • the biological bacteriostatic agent provided by the present invention combines chitosan lactate with ⁇ -polylysine hydrochloride and natamycin, and the effect is much better than that of chitosan hydrochloride or shell.
  • the fresh-keeping effect of the formula of polysaccharide acetate or chitosan, ⁇ -polylysine hydrochloride and natamycin is also far better than that of chitosan, ⁇ -polylysine hydrochloride and natamycin
  • the fresh-keeping effect of the preservative formula composed of vitamins and other preservatives or antioxidants, with fewer components and simpler composition, has achieved a more excellent fresh-keeping effect.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Nutrition Science (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Plasma & Fusion (AREA)
  • Toxicology (AREA)
  • Mycology (AREA)
  • Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention belongs to the field of freshness preservation, and in particular relates to a biological antimicrobial agent. Active ingredients of the antimicrobial bacteriostatic agent comprise chitosan lactate, epsilon-polylysine or epsilon-polylysine salt and natamycin. The biological antimicrobial agent provided by the present invention is healthy, safe, non-toxic and odorless, and the preparation method therefor is simple. When the biological antimicrobial agent is used for keeping fresh Cordyceps sinensis fresh, the refrigeration shelf life of the fresh Cordyceps sinensis can reach 60 days or above, without modified atmosphere packaging being necessary, and the biological antibacterial agent is simple to operate, low cost and easily popularized.

Description

一种生物型抑菌剂及其在鲜冬虫夏草保鲜中的应用A biological type bacteriostatic agent and its application in the preservation of fresh Cordyceps sinensis 技术领域Technical field
本发明涉及保鲜领域，具体涉及一种生物型抑菌剂及其在鲜冬虫夏草保鲜中的应用。The invention relates to the field of preservation, in particular to a biological type bacteriostatic agent and its application in the preservation of fresh cordyceps.
背景技术Background technique
新鲜冬虫夏草含水量高，在冷藏条件下很难抑制腐败微生物的生长，导致品质很快下降。专利文献CN110628633A公开了一种鲜冬虫夏草的保鲜方法及鲜冬虫夏草制品，采用气调包装方式，通过填充50-100体积％氧气、0-40体积％二氧化碳和0-50体积％氮气的气体比例，在0-10℃的冷藏条件下保鲜。专利文献CN109717346A公开了一种辐照法保鲜冬虫夏草的方法，先用保鲜剂浸泡，再气调包装，最后辐照灭菌，在0～4℃的冷藏条件下保鲜。可见，现有技术一般是采用气调包装或者抑菌剂结合气调包装的方式，才能让鲜冬虫夏草在冷藏条件下保存较长的时间，但是气调包装设备成本较高，操作繁多，不利于商业推广。Fresh Cordyceps has a high water content, and it is difficult to inhibit the growth of spoilage microorganisms under cold storage conditions, resulting in a rapid decline in quality. Patent document CN110628633A discloses a method for preserving fresh Cordyceps sinensis and fresh Cordyceps sinensis products. It adopts a modified atmosphere packaging method and is filled with a gas ratio of 50-100% by volume of oxygen, 0-40% by volume of carbon dioxide and 0-50% by volume of nitrogen. Keep fresh under refrigeration at 0-10℃. Patent document CN109717346A discloses a method of radiation preservation of Cordyceps sinensis, which is first soaked with a preservative, then packaged in a modified atmosphere, and finally sterilized by irradiation, and kept fresh under refrigerated conditions at 0-4°C. It can be seen that the prior art generally adopts modified atmosphere packaging or bacteriostatic agent combined with modified atmosphere packaging to keep fresh Cordyceps under refrigerated conditions for a longer period of time. However, the cost of modified atmosphere packaging equipment is relatively high and there are many operations, which is not conducive to Commercial promotion.
目前，亟待开发一种无需进行气调包装就能实现优异效果的抑菌剂以及保鲜方法。At present, there is an urgent need to develop a bacteriostatic agent and a fresh-keeping method that can achieve excellent effects without the need for modified atmosphere packaging.
发明内容Summary of the invention
本发明的第一目的是克服现有技术的缺陷，提供一种健康、安全、无毒、无异味，且抑菌效果好、使用方便的生物型抑菌剂。The first objective of the present invention is to overcome the defects of the prior art and provide a biological bacteriostatic agent that is healthy, safe, non-toxic, no peculiar smell, good bacteriostatic effect, and convenient to use.
具体而言，本发明提供的生物型抑菌剂的活性成分包含：壳聚糖乳酸盐，ε-聚赖氨酸或ε-聚赖氨酸盐，以及纳他霉素。Specifically, the active ingredients of the biological bacteriostatic agent provided by the present invention include chitosan lactate, ε-polylysine or ε-polylysine salt, and natamycin.
作为本发明的优选方案，所述生物型抑菌剂的活性成分由壳聚 糖乳酸盐，ε-聚赖氨酸或ε-聚赖氨酸盐，以及纳他霉素组成。As a preferred embodiment of the present invention, the active ingredient of the biological bacteriostatic agent is composed of chitosan lactate, ε-polylysine or ε-polylysine salt, and natamycin.
本发明中的ε-聚赖氨酸盐指ε-聚赖氨酸的有机酸盐或无机酸盐，为可接受的无毒的酸形成的盐，包括但并不限于，无机酸盐如盐酸盐，氢溴酸盐，磷酸盐，硫酸盐，高氯酸盐，和有机酸盐如乙酸盐，乳酸盐，草酸盐，马来酸盐，酒石酸盐，柠檬酸盐，琥珀酸盐，丙二酸盐，或通过书籍文献上所记载的其他方法如离子交换法来得到这些盐等。The ε-polylysine salt in the present invention refers to the organic or inorganic acid salt of ε-polylysine, which is a salt formed by an acceptable non-toxic acid, including, but not limited to, an inorganic acid salt such as a salt Acid salts, hydrobromide, phosphate, sulfate, perchlorate, and organic acid salts such as acetate, lactate, oxalate, maleate, tartrate, citrate, succinic acid Salt, malonate, or other methods described in books and literature such as ion exchange to obtain these salts.
作为本发明的优选方案，ε-聚赖氨酸盐为ε-聚赖氨酸盐酸盐，ε-聚赖氨酸乳酸盐或ε-聚赖氨酸乙酸盐。As a preferred embodiment of the present invention, the ε-polylysine salt is ε-polylysine hydrochloride, ε-polylysine lactate or ε-polylysine acetate.
作为本发明的优选方案，所述生物型抑菌剂中，壳聚糖乳酸盐、ε-聚赖氨酸或ε-聚赖氨酸盐以及纳他霉素的质量比为(1～15)：(0.1～2)：(0.01～0.2)。As a preferred embodiment of the present invention, in the biological bacteriostatic agent, the mass ratio of chitosan lactate, ε-polylysine or ε-polylysine salt and natamycin is (1-15 ): (0.1～2): (0.01～0.2).
作为本发明的优选方案，所述生物型抑菌剂中，壳聚糖乳酸盐、ε-聚赖氨酸或ε-聚赖氨酸盐以及纳他霉素的质量比为(3～10)：(0.1～1)：(0.02～0.15)。As a preferred embodiment of the present invention, in the biological bacteriostatic agent, the mass ratio of chitosan lactate, ε-polylysine or ε-polylysine salt and natamycin is (3-10 ): (0.1~1): (0.02~0.15).
作为本发明的优选方案，所述生物型抑菌剂中，壳聚糖乳酸盐、ε-聚赖氨酸或ε-聚赖氨酸盐以及纳他霉素的质量比为6:1:0.1。As a preferred solution of the present invention, in the biological bacteriostatic agent, the mass ratio of chitosan lactate, ε-polylysine or ε-polylysine salt and natamycin is 6:1: 0.1.
本发明提供的生物抑菌剂中，除了所述活性成分外，还可以包含本领域常规的辅料，以形成特定的剂型。In addition to the active ingredients, the biological antibacterial agent provided by the present invention may also contain conventional auxiliary materials in the art to form a specific dosage form.
作为本发明的优选方案，所述生物抑菌剂为溶液，优选为水溶液。As a preferred embodiment of the present invention, the biological bacteriostatic agent is a solution, preferably an aqueous solution.
作为本发明的优选方案，所述生物型抑菌剂的溶液中，含有如下质量浓度的组分：壳聚糖乳酸盐0.1～1.5％，ε-聚赖氨酸或ε-聚赖氨酸盐0.01～0.2％，纳他霉素0.002～0.02％。As a preferred solution of the present invention, the solution of the biological bacteriostatic agent contains the following components in mass concentration: chitosan lactate 0.1-1.5%, ε-polylysine or ε-polylysine Salt 0.01～0.2%, Natamycin 0.002～0.02%.
作为本发明的优选方案，所述生物型抑菌剂的溶液中，含有如下质量浓度的组分：壳聚糖乳酸盐0.3～1％，ε-聚赖氨酸或ε-聚赖氨酸盐0.01～0.1％，纳他霉素0.002～0.015％。As a preferred solution of the present invention, the solution of the biological bacteriostatic agent contains the following components in mass concentration: chitosan lactate 0.3-1%, ε-polylysine or ε-polylysine Salt 0.01～0.1%, Natamycin 0.002～0.015%.
作为本发明的优选方案，所述生物型抑菌剂的溶液中，含有如下质量浓度的组分：壳聚糖乳酸盐0.6％，ε-聚赖氨酸或ε-聚赖氨酸盐0.1％，纳他霉素0.01％。As a preferred solution of the present invention, the biological bacteriostatic agent solution contains the following components in mass concentration: chitosan lactate 0.6%, ε-polylysine or ε-polylysine salt 0.1 %, natamycin 0.01%.
本发明提供的生物抑菌剂的溶液中，除了所述活性成分以及溶剂外，可以包含其它溶剂剂型中的常规辅料，也可以不包含其它任何辅料。In addition to the active ingredient and the solvent, the solution of the biological antibacterial agent provided by the present invention may contain conventional excipients in other solvent formulations, or may not contain any other excipients.
作为本发明的优选方案，所述生物抑菌剂的溶液由如下质量浓度的组分组成：壳聚糖乳酸盐0.1～1.5％，ε-聚赖氨酸或ε-聚赖氨酸盐0.01～0.2％，纳他霉素0.002～0.015％，余量为溶剂。As a preferred solution of the present invention, the solution of the biological antibacterial agent is composed of the following components in mass concentration: chitosan lactate 0.1 to 1.5%, ε-polylysine or ε-polylysine salt 0.01 ~0.2%, natamycin 0.002 ~ 0.015%, the balance is solvent.
作为本发明的优选方案，所述生物抑菌剂的溶液由如下质量浓度的组分组成：壳聚糖乳酸盐0.3～1％，ε-聚赖氨酸或ε-聚赖氨酸盐0.01～0.1％，纳他霉素0.002～0.015％，余量为溶剂。As a preferred solution of the present invention, the solution of the biological antibacterial agent is composed of the following components in mass concentration: chitosan lactate 0.3-1%, ε-polylysine or ε-polylysine salt 0.01 ~0.1%, natamycin 0.002 ~ 0.015%, the balance is solvent.
作为本发明的优选方案，所述生物抑菌剂的溶液由如下质量浓度的组分组成：壳聚糖乳酸盐0.6％，ε-聚赖氨酸或ε-聚赖氨酸盐0.1％，纳他霉素0.01％，余量为溶剂。As a preferred solution of the present invention, the solution of the biological antibacterial agent is composed of the following components in mass concentration: chitosan lactate 0.6%, ε-polylysine or ε-polylysine 0.1%, Natamycin is 0.01%, the remainder is solvent.
作为本发明的一种具体实施方式，上述优选方案中，所述ε-聚赖氨酸盐为ε-聚赖氨酸盐酸盐。As a specific embodiment of the present invention, in the above preferred embodiment, the ε-polylysine salt is ε-polylysine hydrochloride.
作为本发明的优选方案，所述生物抑菌剂是用于鲜冬虫夏草保鲜的抑菌剂。本发明所述鲜冬虫夏草是指未经过干燥、加热或其它加工处理的新鲜冬虫夏草。As a preferred embodiment of the present invention, the biological bacteriostatic agent is a bacteriostatic agent for preservation of fresh Cordyceps sinensis. The fresh Cordyceps sinensis in the present invention refers to fresh Cordyceps sinensis that has not undergone drying, heating or other processing treatments.
本发明提供的生物型抑菌剂健康、安全、无毒、无异味。且所述抑菌剂的制备方法简单；在制备溶液型抑菌剂时，只需将各组分溶于水即可。该生物抑菌剂对于鲜冬虫夏草的保鲜，具有优异的效果。The bio-type bacteriostatic agent provided by the invention is healthy, safe, non-toxic and has no peculiar smell. In addition, the preparation method of the bacteriostatic agent is simple; when preparing a solution-type bacteriostatic agent, all components only need to be dissolved in water. The biological bacteriostatic agent has excellent effects on the preservation of fresh Cordyceps sinensis.
本发明的第二目的是提供所述生物抑菌剂在鲜中药材保鲜中的应用。The second object of the present invention is to provide the application of the biological antibacterial agent in the preservation of fresh Chinese medicinal materials.
作为本发明的优选方案，所属鲜中药材为鲜冬虫夏草。As a preferred solution of the present invention, the fresh Chinese medicinal material is fresh Cordyceps sinensis.
作为本发明的优选方案，所述保鲜是指低温保鲜，如在0～8℃温度条件下的保鲜。As a preferred solution of the present invention, the preservation refers to preservation at low temperature, such as preservation at a temperature of 0-8°C.
本发明的第三目的是提供一种鲜冬虫夏草的保鲜方法，所述方法包括使用本发明提供的生物型抑菌剂对鲜冬虫夏草进行处理。The third object of the present invention is to provide a method for preserving fresh Cordyceps sinensis, the method comprising using the bio-type bacteriostatic agent provided by the present invention to treat the fresh Cordyceps sinensis.
作为本发明的优选方案，所述保鲜方法包括：先用所述生物型抑菌剂对鲜冬虫夏草进行处理，再对其进行灭菌，然后置于0～8℃保藏。As a preferred solution of the present invention, the preservation method includes: first treating the fresh Cordyceps sinensis with the biological bacteriostatic agent, then sterilizing it, and then storing it at 0-8°C.
当所述生物型抑菌剂为溶液时，所述保鲜方法包括：将所述鲜冬虫夏草置于所述生物型抑菌剂的溶液中浸泡，优选浸泡3～15min，取出沥干，灭菌，然后置于0～8℃保藏。When the biological bacteriostatic agent is a solution, the preservation method includes: immersing the fresh cordyceps in the biological bacteriostatic agent solution, preferably soaking for 3-15 minutes, taking it out, draining, and sterilizing, Then store at 0-8°C.
本发明所述灭菌优选为辐照灭菌，如采用1.8KGy电子束辐照灭菌。The sterilization in the present invention is preferably irradiation sterilization, such as using 1.8 KGy electron beam irradiation sterilization.
采用本发明提供的方法对鲜冬虫夏草进行保鲜，可以确保在储存到60天时鲜冬虫夏草中的金黄色葡萄球菌、沙门氏菌、大肠埃希氏菌、大肠菌群以及总菌落数都符合食品中致病菌的限量标准，使鲜冬虫夏草的冷藏保质期达到60天以上。且本发明提供的保鲜方法处理过程简单，无需进行气调包装，成本低、易推广。Adopting the method provided by the present invention to keep fresh Cordyceps sinensis can ensure that Staphylococcus aureus, Salmonella, Escherichia coli, coliforms and total colony counts in fresh Cordyceps are in line with pathogenic bacteria in food when stored for 60 days The limit standard of, the cold storage shelf life of fresh Cordyceps can reach more than 60 days. In addition, the fresh-keeping method provided by the present invention has a simple processing process, does not require modified atmosphere packaging, has low cost, and is easy to popularize.
具体实施方式Detailed ways
以下实施例用于说明本发明，但不用来限制本发明的范围。实施例中未注明具体技术或条件的，按照本领域内的文献所描述的技术或条件或者按照产品说明书进行。所用试剂或仪器未注明生产厂商者，均为可以通过市购获得的常规产品。The following examples are used to illustrate the present invention, but not to limit the scope of the present invention. Where specific techniques or conditions are not indicated in the examples, the procedures shall be carried out in accordance with the techniques or conditions described in the literature in the field or in accordance with the product specification. The reagents or instruments used without the manufacturer's indication are all conventional products that can be purchased commercially.
以下具体实施方式参考《DB43/T 470-2009净菜通则》凉菜类净菜和《GB29921-2013食品中致病菌限量》即食果蔬制品类标准要求，并结合工艺及产品自身特点，制定了微生物检测项目及标准。如表1所示。The following specific implementation methods refer to the standard requirements of "DB43/T 470-2009 Clean Vegetables General Rules" Cold Vegetables Clean Vegetables and "GB29921-2013 Limits of Pathogenic Bacteria in Foods" Ready-to-eat Fruit and Vegetable Products Standards, and combine the process and product characteristics to develop microbiological testing items And standards. As shown in Table 1.
表1：微生物检测项目及标准Table 1: Microbiological testing items and standards
检测项目Test items 可接受标准Acceptable standard 检验方法Testing method
金黄色葡萄球菌Staphylococcus aureus ≤100CFU/g≤100CFU/g GB4789.10-2016第二法GB4789.10-2016 Second Law
沙门氏菌salmonella 不得检出/25gNot detectable/25g GB4789.4-2016GB4789.4-2016
大肠埃希氏菌O157：H7Escherichia coli O157: H7 不得检出/25gNot detectable/25g GB4789.36-2016GB4789.36-2016
大肠菌群Coliform ≤90MPN/100g≤90MPN/100g GB4789.3-2003GB4789.3-2003
菌落总数Total number of colonies ≤104CFU/g≤104CFU/g GB4789.2-2016GB4789.2-2016
以下具体实施方式中，处理对象新鲜冬虫夏草来源于宜都山城水都冬虫夏草有限公司；壳聚糖乳酸盐，脱乙酰度85％以上，购于青岛弘海科技有限公司；ε-聚赖氨酸盐酸盐、纳他霉素购于郑州拜纳佛生物工程股份有限公司；茶多酚、氯化钠购于浙江一诺生物科技有限公司；对羟基苯甲酸酯购于浙江圣效化学品有限公司。In the following specific embodiments, the fresh Cordyceps sinensis to be treated comes from Yidu Shancheng Shuidu Cordyceps Co., Ltd.; chitosan lactate, with a degree of deacetylation of over 85%, purchased from Qingdao Honghai Technology Co., Ltd.; ε-polylysine salt Acetate and natamycin were purchased from Zhengzhou Binafo Bioengineering Co., Ltd.; tea polyphenols and sodium chloride were purchased from Zhejiang Yinuo Biotechnology Co., Ltd.; parabens were purchased from Zhejiang Shengxiao Chemical Co., Ltd. company.
以下具体实施方式中，各种仪器、工具都提前进行灭菌处理。In the following specific embodiments, various instruments and tools are sterilized in advance.
实施例1Example 1
本实施例提供了一种生物型抑菌剂。所述抑菌剂为水溶液，其由如下浓度的组分组成：壳聚糖乳酸盐3g/kg，ε-聚赖氨酸盐酸盐0.1g/kg，纳他霉素0.02g/kg。This embodiment provides a biological bacteriostatic agent. The bacteriostatic agent is an aqueous solution, which is composed of components with the following concentrations: chitosan lactate 3g/kg, ε-polylysine hydrochloride 0.1g/kg, and natamycin 0.02g/kg.
实施例2Example 2
本实施例提供了一种生物型抑菌剂。所述抑菌剂为水溶液，其由如下浓度的组分组成：壳聚糖乳酸盐10g/kg，ε-聚赖氨酸盐酸盐1g/kg，纳他霉素0.15g/kg。This embodiment provides a biological bacteriostatic agent. The bacteriostatic agent is an aqueous solution, which is composed of components with the following concentrations: chitosan lactate 10g/kg, ε-polylysine hydrochloride 1g/kg, and natamycin 0.15g/kg.
实施例3Example 3
本实施例提供了一种生物型抑菌剂。所述抑菌剂为水溶液，其由如下浓度的组分组成：壳聚糖乳酸盐6g/kg，ε-聚赖氨酸盐酸盐1g/kg，纳他霉素0.1g/kg。This embodiment provides a biological bacteriostatic agent. The bacteriostatic agent is an aqueous solution, which consists of the following components at a concentration of 6 g/kg of chitosan lactate, 1 g/kg of ε-polylysine hydrochloride, and 0.1 g/kg of natamycin.
对比例1Comparative example 1
本对比例提供了一种生物型抑菌剂。与实施例3相比，区别仅在于：用壳聚糖盐酸盐代替所述壳聚糖乳酸盐。This comparative example provides a biological type bacteriostatic agent. Compared with Example 3, the only difference is that chitosan hydrochloride is used instead of the chitosan lactate.
对比例2Comparative example 2
本对比例提供了一种生物型抑菌剂。与实施例3相比，区别仅在于：用壳聚糖乙酸盐代替所述壳聚糖乳酸盐。This comparative example provides a biological type bacteriostatic agent. Compared with Example 3, the only difference is that chitosan acetate is used instead of the chitosan lactate.
对比例3Comparative example 3
本对比例提供了一种生物型抑菌剂。与实施例3相比，区别仅在于：用壳聚糖代替所述壳聚糖乳酸盐。This comparative example provides a biological type bacteriostatic agent. Compared with Example 3, the only difference is that chitosan is used instead of the chitosan lactate.
对比例4Comparative example 4
本对比例提供了一种生物型抑菌剂。所述抑菌剂为水溶液，其由如下浓度的组分组成：壳聚糖6g/kg、ε-聚赖氨酸盐酸盐1g/kg、纳他霉素0.1g/kg、对羟基苯甲酸酯0.05％。This comparative example provides a biological type bacteriostatic agent. The bacteriostatic agent is an aqueous solution, which is composed of the following components: chitosan 6g/kg, ε-polylysine hydrochloride 1g/kg, natamycin 0.1g/kg, p-hydroxybenzyl Acid ester 0.05%.
对比例5Comparative example 5
本对比例提供了一种生物型抑菌剂。所述抑菌剂为水溶液，其由如下浓度的组分组成：壳聚糖6g/kg、ε-聚赖氨酸盐酸盐1g/kg、纳他霉素0.1g/kg、氯化钠1％。This comparative example provides a biological type bacteriostatic agent. The bacteriostatic agent is an aqueous solution, which is composed of the following components: chitosan 6g/kg, ε-polylysine hydrochloride 1g/kg, natamycin 0.1g/kg, sodium chloride 1 %.
对比例6Comparative example 6
本对比例提供了一种生物型抑菌剂。所述抑菌剂为水溶液，其由如下浓度的组分组成：壳聚糖6g/kg、ε-聚赖氨酸盐酸盐1g/kg、纳他霉素0.1g/kg、醋酸0.2％、茶多酚0.03％、葡萄糖氧化酶0.05％。This comparative example provides a biological type bacteriostatic agent. The bacteriostatic agent is an aqueous solution, which is composed of the following components: chitosan 6g/kg, ε-polylysine hydrochloride 1g/kg, natamycin 0.1g/kg, acetic acid 0.2%, Tea polyphenols 0.03%, glucose oxidase 0.05%.
对比例7Comparative example 7
本对比例提供的生物型抑菌剂为单组份水溶液，具体为：The biological bacteriostatic agent provided by this comparative example is a single-component aqueous solution, specifically:
对比例7-1：浓度为6g/kg的壳聚糖盐酸盐；Comparative Example 7-1: Chitosan hydrochloride with a concentration of 6g/kg;
对比例7-2：浓度为6g/kg的壳聚糖乳酸盐；Comparative Example 7-2: Chitosan lactate with a concentration of 6g/kg;
对比例7-3：浓度为6g/kg的壳聚糖乙酸盐；Comparative Example 7-3: Chitosan acetate with a concentration of 6g/kg;
对比例7-4：浓度为1g/kg的ε-聚赖氨酸盐酸盐；Comparative Example 7-4: ε-polylysine hydrochloride with a concentration of 1g/kg;
对比例7-5：浓度为0.1g/kg的纳他霉素；Comparative example 7-5: Natamycin with a concentration of 0.1g/kg;
对比例7-6：浓度为6g/kg的壳聚糖。Comparative Example 7-6: Chitosan with a concentration of 6g/kg.
实验例1Experimental example 1
取实施例1提供的生物型抑菌剂溶液，将120根清洗干净的新 鲜冬虫夏草放入其中浸泡处理3min，取出后沥干，分别装入120支玻璃管中，并用1.8KGy电子束辐照后，放置0℃冰箱中保藏。每隔15天取出20根测定微生物含量。结果如表2所示。Take the biological bacteriostatic agent solution provided in Example 1, put 120 cleaned fresh Cordyceps into it for 3 minutes, take it out and drain, put them into 120 glass tubes, and irradiate them with 1.8KGy electron beam , Placed in the refrigerator at 0 ℃ preservation. Take out 20 roots every 15 days to determine the microbial content. The results are shown in Table 2.
表2：微生物含量测定结果Table 2: Microbial content determination results
检测项目Test items 0d0d 15d15d 30d30d 45d45d 60d60d 75d75d
金黄色葡萄球菌Staphylococcus aureus 合格qualified 合格qualified 合格qualified 合格qualified 合格qualified 合格qualified
沙门氏菌salmonella 合格qualified 合格qualified 合格qualified 合格qualified 合格qualified 合格qualified
大肠埃希氏菌O157：H7Escherichia coli O157: H7 合格qualified 合格qualified 合格qualified 合格qualified 合格qualified 合格qualified
大肠菌群Coliform 合格qualified 合格qualified 合格qualified 合格qualified 合格qualified 不合格Unqualified
菌落总数Total number of colonies 合格qualified 合格qualified 合格qualified 合格qualified 合格qualified 不合格Unqualified
由表2结果可知，经实施例1提供的生物抑菌剂处理的鲜冬虫夏草再辐照灭菌，冷藏保质期可以达到60天(各项检测指标都合格)。From the results in Table 2, it can be seen that the fresh Cordyceps sinensis treated with the biological antibacterial agent provided in Example 1 is irradiated and sterilized, and the shelf life of cold storage can reach 60 days (all test indicators are qualified).
实验例2Experimental example 2
取实施例2提供的生物型抑菌剂溶液，将120根清洗干净的新鲜冬虫夏草放入其中浸泡处理15min，取出后沥干，分别装入120支玻璃管中，并用1.8KGy电子束辐照后，放置8℃冰箱中保藏。每隔15天取出20根测定微生物含量。结果如表3所示。Take the biological bacteriostatic agent solution provided in Example 2, put 120 cleaned fresh Cordyceps into it and soak for 15min, take it out and drain, put them into 120 glass tubes, and irradiate them with 1.8KGy electron beam , Placed in a refrigerator at 8 ℃ preservation. Take out 20 roots every 15 days to determine the microbial content. The results are shown in Table 3.
表3：微生物含量测定结果Table 3: Microbial content determination results
检测项目Test items 0d0d 15d15d 30d30d 45d45d 60d60d 75d75d
金黄色葡萄球菌Staphylococcus aureus 合格qualified 合格qualified 合格qualified 合格qualified 合格qualified 合格qualified
沙门氏菌salmonella 合格qualified 合格qualified 合格qualified 合格qualified 合格qualified 合格qualified
大肠埃希氏菌O157：H7Escherichia coli O157: H7 合格qualified 合格qualified 合格qualified 合格qualified 合格qualified 合格qualified
大肠菌群Coliform 合格qualified 合格qualified 合格qualified 合格qualified 合格qualified 不合格Unqualified
菌落总数Total number of colonies 合格qualified 合格qualified 合格qualified 合格qualified 合格qualified 合格qualified
由表3结果可知，经实施例2提供的生物抑菌剂处理的鲜冬虫夏草再辐照灭菌，冷藏保质期可以达到60天(各项检测指标都合格)。From the results in Table 3, it can be seen that the fresh Cordyceps sinensis treated with the biological bacteriostatic agent provided in Example 2 is irradiated and sterilized, and the shelf life of cold storage can reach 60 days (all test indicators are qualified).
实验例3Experimental example 3
取实施例3提供的生物型抑菌剂溶液，将120根清洗干净的新 鲜冬虫夏草放入其中浸泡处理5min，取出后沥干，分别装入120支玻璃管中，并用1.8KGy电子束辐照后，放置4℃冰箱中保藏。每隔15天取出20根测定微生物含量。Take the biological bacteriostatic agent solution provided in Example 3, put 120 cleaned fresh Cordyceps into it for 5 minutes, take it out and drain, put them into 120 glass tubes, and irradiate them with 1.8KGy electron beam , Placed in a refrigerator at 4℃ for preservation. Take out 20 roots every 15 days to determine the microbial content.
作为对照，先将120根洗净的新鲜冬虫夏草经1.8KGy电子束辐照灭菌，然后放入实施例3提供的生物型抑菌剂溶液(已经过灭菌处理)中浸泡处理5min，取出后沥干，分别装入120支玻璃管中，放置4℃冰箱中保藏。每隔15天取出20根测定微生物含量。As a control, first 120 cleaned fresh Cordyceps were sterilized by 1.8KGy electron beam irradiation, and then put into the bio-type bacteriostatic agent solution provided in Example 3 (which had been sterilized) for 5 minutes, and then removed. Drain them, put them into 120 glass tubes, and store them in a refrigerator at 4°C. Take out 20 roots every 15 days to determine the microbial content.
测定结果如表4所示。The measurement results are shown in Table 4.
表4：微生物含量测定结果Table 4: Microbial content determination results
Figure PCTCN2020093021-appb-000001
Figure PCTCN2020093021-appb-000001
由表4的结果可知，经实施例3提供的生物抑菌剂处理的鲜冬虫夏草再辐照灭菌，冷藏保质期可以达到60天(各项检测指标都合格)。而先灭菌处理再用抑菌剂浸泡处理的方式在第45天就开始出现指标不合格(一旦出现不合格，后续不再检测)，保质期仅可达到30天。可见，先经抑菌剂浸泡处理再进行灭菌处理的方式，抑菌效果显著优于先灭菌处理再用抑菌剂浸泡处理的方式。From the results in Table 4, it can be seen that the fresh Cordyceps sinensis treated with the biological antibacterial agent provided in Example 3 is irradiated and sterilized, and the shelf life of refrigeration can reach 60 days (all test indicators are qualified). However, the method of sterilizing first and then immersing the bacteriostatic agent in the index began to appear unqualified on the 45th day (Once unqualified, subsequent testing will not be performed), and the shelf life can only reach 30 days. It can be seen that the method of first immersing the bacteriostatic agent and then performing the sterilization treatment has a significantly better antibacterial effect than the method of first sterilizing and then immersing the bacteriostatic agent.
实验例4Experimental example 4
先将洗净的新鲜冬虫夏草经1.8KGy电子束辐照灭菌，然后分别放入实施例3以及对比例1～7提供的已经过灭菌处理的生物型抑菌剂溶液中(每种抑菌剂溶液中各放120根)浸泡处理5min，取出后沥干，分别装入玻璃管中，放置4℃冰箱中保藏。第21天起，每隔7天分别从每种抑菌剂溶液中各取出20根测定微生物含量。结果如表5所示。First, the washed fresh Cordyceps was sterilized by 1.8KGy electron beam irradiation, and then put into the sterilized biological bacteriostatic solution provided in Example 3 and Comparative Examples 1-7 (each type of bacteriostatic Put 120 pieces in each of the reagent solution) soak for 5min, take it out and drain, put them into glass tubes, and place them in a refrigerator at 4°C for storage. From the 21st day, 20 roots were taken from each bacteriostatic solution every 7 days to determine the microbial content. The results are shown in Table 5.
表5：微生物含量测定结果Table 5: Microbial content determination results
Figure PCTCN2020093021-appb-000002
Figure PCTCN2020093021-appb-000002
Figure PCTCN2020093021-appb-000003
Figure PCTCN2020093021-appb-000003
Figure PCTCN2020093021-appb-000004
Figure PCTCN2020093021-appb-000004
Figure PCTCN2020093021-appb-000005
Figure PCTCN2020093021-appb-000005
由表5的结果可知，灭菌后的鲜冬虫夏草经实施例3提供的抑菌剂处理后冷藏保存，冷藏保质期可达到42天(各项检测指标都合格)。It can be seen from the results in Table 5 that the sterilized fresh Cordyceps sinensis is treated with the bacteriostatic agent provided in Example 3 and stored in a cold storage, and the cold storage shelf life can reach 42 days (all test indicators are qualified).
相比较而言，灭菌后的鲜冬虫夏草经对比例1、对比例2以及对比例3提供的抑菌剂处理后冷藏保存，分别在第35天就开始出现指标不合格(一旦出现不合格，后续不再检测)，保质期仅可达到28天。In comparison, the sterilized fresh Cordyceps sinensis was treated with the bacteriostatic agent provided in Comparative Example 1, Comparative Example 2 and Comparative Example 3 and then stored under refrigeration. The indicators began to be unqualified on the 35th day (Once unqualified, No further testing), the shelf life can only reach 28 days.
相比较而言，灭菌后的鲜冬虫夏草经对比例4、对比例5以及对比例6提供的抑菌剂处理后冷藏保存，分别在第35天就开始出现指标不合格(一旦出现不合格，后续不再检测)，保质期仅可达到28天。且对比例4中对羟基苯甲酸酯是化学防腐剂不够安全，对比例5中的氯化钠以及对比例6中的醋酸会改变鲜冬虫夏草的滋味。In comparison, the sterilized fresh Cordyceps sinensis was treated with the bacteriostatic agent provided in Comparative Example 4, Comparative Example 5, and Comparative Example 6 and stored under refrigeration. The indicators began to be unqualified on the 35th day (Once unqualified, No further testing), the shelf life can only reach 28 days. Moreover, the parabens in Comparative Example 4 are not safe enough as chemical preservatives. The sodium chloride in Comparative Example 5 and the acetic acid in Comparative Example 6 will change the taste of fresh Cordyceps sinensis.
相比较而言，灭菌后的鲜冬虫夏草经对比例7(6组单一活性成分的抑菌剂)处理后，冷藏保质期不长于28天；其中对比例7-6，即浓度为6g/kg的壳聚糖水溶液处理，冷藏保质期仅能达到21天。In comparison, after sterilization of fresh Cordyceps sinensis treated by Comparative Example 7 (6 groups of bacteriostatic agents with a single active ingredient), the shelf life of cold storage is not longer than 28 days; Among them, Comparative Examples 7-6, that is, the concentration of 6g/kg The shelf life of chitosan aqueous solution can only reach 21 days in cold storage.
综上所述，本发明提供的生物型抑菌剂将壳聚糖乳酸盐与ε-聚赖氨酸盐酸盐和纳他霉素合用，效果远优于壳聚糖盐酸盐或壳聚糖乙酸盐或壳聚糖与ε-聚赖氨酸盐酸盐和纳他霉素配方的保鲜效果，也远优于壳聚糖与ε-聚赖氨酸盐酸盐和纳他霉素以及其他防腐剂或抗氧化剂等一起组成的保鲜剂配方的保鲜效果，在组分更少、组成更简单的情况下，取得了更加优异的保鲜效果。In summary, the biological bacteriostatic agent provided by the present invention combines chitosan lactate with ε-polylysine hydrochloride and natamycin, and the effect is much better than that of chitosan hydrochloride or shell. The fresh-keeping effect of the formula of polysaccharide acetate or chitosan, ε-polylysine hydrochloride and natamycin is also far better than that of chitosan, ε-polylysine hydrochloride and natamycin The fresh-keeping effect of the preservative formula composed of vitamins and other preservatives or antioxidants, with fewer components and simpler composition, has achieved a more excellent fresh-keeping effect.
虽然，上文中已经用一般性说明、具体实施方式及试验，对本发 明作了详尽的描述，但在本发明基础上，可以对之作一些修改或改进，这对本领域技术人员而言是显而易见的。因此，在不偏离本发明精神的基础上所做的这些修改或改进，均属于本发明要求保护的范围。Although the general description, specific embodiments and experiments have been used to describe the present invention in detail, some modifications or improvements can be made on the basis of the present invention, which is obvious to those skilled in the art. . Therefore, these modifications or improvements made without departing from the spirit of the present invention all belong to the scope of the present invention.

Claims (10)

  1. 一种生物型抑菌剂，其特征在于，其活性成分包含：壳聚糖乳酸盐，ε-聚赖氨酸或ε-聚赖氨酸盐，以及纳他霉素。A biological antibacterial agent, characterized in that its active ingredients include chitosan lactate, ε-polylysine or ε-polylysine salt, and natamycin.
  2. 根据权利要求1所述的生物型抑菌剂，其特征在于，其活性成分由壳聚糖乳酸盐、ε-聚赖氨酸或ε-聚赖氨酸盐，以及纳他霉素组成。The biological bacteriostatic agent according to claim 1, characterized in that its active ingredient is composed of chitosan lactate, ε-polylysine or ε-polylysine salt, and natamycin.
  3. 根据权利要求1或2所述的生物型抑菌剂，其特征在于，所述ε-聚赖氨酸盐为ε-聚赖氨酸盐酸盐，ε-聚赖氨酸乳酸盐或ε-聚赖氨酸乙酸盐。The biological bacteriostatic agent according to claim 1 or 2, wherein the ε-polylysine salt is ε-polylysine hydrochloride, ε-polylysine lactate or ε-polylysine lactate. -Polylysine acetate.
  4. 根据权利要求1～3任意一项所述的生物型抑菌剂，其特征在于，所述壳聚糖乳酸盐、ε-聚赖氨酸或ε-聚赖氨酸盐以及纳他霉素的质量比为(1～15)：(0.1～2)：(0.01～0.2)，优选为(3～10)：(0.1～1)：(0.02～0.15)，更优选为6:1:0.1。The biological bacteriostatic agent according to any one of claims 1 to 3, wherein the chitosan lactate, ε-polylysine or ε-polylysine salt and natamycin The mass ratio is (1-15): (0.1-2): (0.01-0.2), preferably (3-10): (0.1-1): (0.02-0.15), more preferably 6:1:0.1 .
  5. 根据权利要求1～4任意一项所述的生物型抑菌剂，其特征在于，所述生物抑菌剂为溶液，优选为水溶液。The biological bacteriostatic agent according to any one of claims 1 to 4, wherein the biological bacteriostatic agent is a solution, preferably an aqueous solution.
  6. 根据权利要求1～5任意一项所述的生物型抑菌剂，其特征在于，所述生物型抑菌剂的溶液中，含有如下质量浓度的组分：壳聚糖乳酸盐0.1～1.5％，ε-聚赖氨酸或ε-聚赖氨酸盐0.01～0.2％，纳他霉素0.002～0.02％；The biological bacteriostatic agent according to any one of claims 1 to 5, wherein the solution of the biological bacteriostatic agent contains the following components in mass concentration: chitosan lactate 0.1 to 1.5 %, ε-polylysine or ε-polylysine salt 0.01～0.2%, natamycin 0.002～0.02%;
    优选地，含有如下质量浓度的组分：壳聚糖乳酸盐0.3～1％，ε-聚赖氨酸或ε-聚赖氨酸盐0.01～0.1％，纳他霉素0.002～0.015％；Preferably, it contains the following components in mass concentration: chitosan lactate 0.3 to 1%, ε-polylysine or ε-polylysine salt 0.01 to 0.1%, and natamycin 0.002 to 0.015%;
    更优选地，含有如下质量浓度的组分：壳聚糖乳酸盐0.6％，ε-聚赖氨酸或ε-聚赖氨酸盐0.1％，纳他霉素0.01％。More preferably, it contains the following components in mass concentration: chitosan lactate 0.6%, ε-polylysine or ε-polylysine salt 0.1%, and natamycin 0.01%.
  7. 根据权利要求1～6任意一项所述的生物型抑菌剂，其特征在于，所述生物抑菌剂的溶液由如下质量浓度的组分组成：壳聚糖乳酸盐0.1～1.5％，ε-聚赖氨酸或ε-聚赖氨酸盐0.01～0.2％，纳他霉素0.002～0.02％，余量为溶剂；The biological bacteriostatic agent according to any one of claims 1 to 6, characterized in that the solution of the biological bacteriostatic agent is composed of the following components in mass concentration: chitosan lactate 0.1 to 1.5%, ε-polylysine or ε-polylysine salt 0.01～0.2%, natamycin 0.002～0.02%, the balance is solvent;
    优选地，所述生物抑菌剂的溶液由如下质量浓度的组分组成：壳 聚糖乳酸盐0.3～1％，ε-聚赖氨酸或ε-聚赖氨酸盐0.01～0.1％，纳他霉素0.002～0.015％，余量为溶剂；Preferably, the solution of the biological antibacterial agent is composed of the following components in mass concentration: chitosan lactate 0.3-1%, ε-polylysine or ε-polylysine salt 0.01-0.1%, Natamycin 0.002～0.015%, the balance is solvent;
    更优选地，所述生物抑菌剂的溶液由如下质量浓度的组分组成：壳聚糖乳酸盐0.6％，ε-聚赖氨酸或ε-聚赖氨酸盐0.1％，纳他霉素0.01％，余量为溶剂。More preferably, the solution of the biological antibacterial agent is composed of the following components in mass concentration: chitosan lactate 0.6%, ε-polylysine or ε-polylysine salt 0.1%, natamycin 0.01%, the balance is solvent.
  8. 权利要求1～7任意一项所述生物抑菌剂在鲜中药材保鲜中的应用；优选地，所述鲜中药材为鲜冬虫夏草。Application of the biological bacteriostatic agent according to any one of claims 1 to 7 in the preservation of fresh Chinese medicinal materials; preferably, the fresh Chinese medicinal materials are fresh Cordyceps sinensis.
  9. 一种鲜冬虫夏草的保鲜方法，其特征在于，所述方法包括使用权利要求1～7任意一项所述生物抑菌剂对鲜冬虫夏草进行处理；A method for preserving fresh Cordyceps sinensis, characterized in that the method comprises using the biological antibacterial agent according to any one of claims 1 to 7 to treat fresh Cordyceps sinensis;
    优选地，先用所述生物型抑菌剂对鲜冬虫夏草进行处理，再对其进行灭菌，然后置于0～8℃保藏。Preferably, the fresh Cordyceps sinensis is first treated with the biological bacteriostatic agent, then sterilized, and then stored at 0-8°C.
  10. 根据权利要求9所述的保鲜方法，其特征在于，将所述鲜冬虫夏草置于所述生物型抑菌剂的溶液中浸泡，优选浸泡3～15min，取出沥干，灭菌，然后置于0～8℃保藏。The preservation method according to claim 9, wherein the fresh Cordyceps is soaked in the solution of the biological bacteriostatic agent, preferably soaked for 3-15 minutes, taken out, drained, sterilized, and then placed in 0 Store at ~8°C.
PCT/CN2020/093021 2020-04-10 2020-05-28 Biological antimicrobial agent and use thereof in keeping fresh cordyceps sinensis fresh WO2021203539A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202010280871.9 2020-04-10
CN202010280871.9A CN111493266B (en) 2020-04-10 2020-04-10 Biological bacteriostatic agent and application thereof in fresh keeping of fresh cordyceps sinensis

Publications (1)

Publication Number Publication Date
WO2021203539A1 true WO2021203539A1 (en) 2021-10-14

Family

ID=71848236

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2020/093021 WO2021203539A1 (en) 2020-04-10 2020-05-28 Biological antimicrobial agent and use thereof in keeping fresh cordyceps sinensis fresh

Country Status (2)

Country Link
CN (1) CN111493266B (en)
WO (1) WO2021203539A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114468082A (en) * 2021-12-23 2022-05-13 潍坊科技学院 Tea preservative and preparation method thereof
CN115777775A (en) * 2022-11-28 2023-03-14 兰州大学 Postharvest fresh-keeping storage method for fresh pilose asiabell roots

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11196765A (en) * 1998-01-20 1999-07-27 Okayama Oyo Kagaku:Kk Agent for maintaining freshness
CN101911967A (en) * 2010-08-20 2010-12-15 山东商业职业技术学院 Young garlic shoot preservative
CN102188034A (en) * 2010-03-03 2011-09-21 天津科技大学 Compound biological antiseptic and preservation agent for food
CN104082830A (en) * 2014-06-11 2014-10-08 南通昊友食品添加剂有限公司 Composite biological antiseptic antistaling agent
CN104542961A (en) * 2015-01-05 2015-04-29 天津商业大学 Method for preserving fresh walnut kernels
CN110122730A (en) * 2019-06-21 2019-08-16 广东东阳光药业有限公司 The fresh jelly liquid of one kind, its preservation method and its application

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20180193209A1 (en) * 2015-07-07 2018-07-12 Singapore Health Services Pte Ltd. A polymer product and preparation therof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11196765A (en) * 1998-01-20 1999-07-27 Okayama Oyo Kagaku:Kk Agent for maintaining freshness
CN102188034A (en) * 2010-03-03 2011-09-21 天津科技大学 Compound biological antiseptic and preservation agent for food
CN101911967A (en) * 2010-08-20 2010-12-15 山东商业职业技术学院 Young garlic shoot preservative
CN104082830A (en) * 2014-06-11 2014-10-08 南通昊友食品添加剂有限公司 Composite biological antiseptic antistaling agent
CN104542961A (en) * 2015-01-05 2015-04-29 天津商业大学 Method for preserving fresh walnut kernels
CN110122730A (en) * 2019-06-21 2019-08-16 广东东阳光药业有限公司 The fresh jelly liquid of one kind, its preservation method and its application

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114468082A (en) * 2021-12-23 2022-05-13 潍坊科技学院 Tea preservative and preparation method thereof
CN115777775A (en) * 2022-11-28 2023-03-14 兰州大学 Postharvest fresh-keeping storage method for fresh pilose asiabell roots
CN115777775B (en) * 2022-11-28 2024-04-05 兰州大学 Postharvest fresh-keeping storage method for codonopsis pilosula with fresh lines

Also Published As

Publication number Publication date
CN111493266B (en) 2021-09-28
CN111493266A (en) 2020-08-07

Similar Documents

Publication Publication Date Title
CN107624859B (en) Crayfish compound preservative and crayfish preservation treatment method
WO2021203539A1 (en) Biological antimicrobial agent and use thereof in keeping fresh cordyceps sinensis fresh
CN107136200A (en) Vegetable cold chain logistics preservation method
BR112017010669B1 (en) ANTIMICROBIAL COMPOSITION, METHOD TO REDUCE THE NUMBER OF PATHOGENS ON A FOOD SURFACE AND METHOD TO REDUCE THE NUMBER OF PATHOGENS IN A VOLUME OF A LIQUID FOOD PRODUCT
US20070237671A1 (en) Novel method of sanitizing target items using a moist sanitizing gas
JPH08289768A (en) Antimicrobial agent for food
CN111357802A (en) Preparation method of fresh conditioning chicken product and natural water-retention preservative thereof
Kim et al. Calcinated calcium and mild heat treatment on storage quality and microbial populations of fresh-cut iceberg lettuce
CN107484816A (en) A kind of Chinese toon storage fresh-keeping and processing method
CN101828590A (en) Method for refreshing and conditioning fresh meat product by using edible gelatine bacteriostatic coating
KR101761241B1 (en) Method of processing vegetables enabling long-term storage and seasoning liquid used in the process
CN110367328B (en) Preparation method of egg white liquid with long shelf life
Ingram Combination processes
KR100576333B1 (en) Method for keeping kimchi from overfermenting
CN112137010A (en) Biological color-protecting and fresh-keeping method for fresh green peppers
CN112515084A (en) Irradiation sterilization method for frozen product
CN111034781A (en) Composite preservative for meat products and application thereof
Shi et al. Ultrasound treatment alleviates external pericarp browning and improves fruit quality of pomegranate during storage
CN114747614B (en) Biological safety preservative paper for fruits and vegetables and preparation method thereof
CN113508882B (en) Sterilizing process of coconut water
Glevitzky et al. Studies regarding the use of preservatives on soft drinks stability
WO2024130722A1 (en) Method for synergistically treating fresh-cut fruit and vegetables by means of plasma-activated water and tartaric acid and preserving freshness of same
KR950008869B1 (en) Processing of kimchi content
CN114258940A (en) Method for purifying and preserving chilled meat
CN107318945A (en) A kind of production method of cold fresh goose

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 20929930

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 20929930

Country of ref document: EP

Kind code of ref document: A1

32PN Ep: public notification in the ep bulletin as address of the adressee cannot be established

Free format text: NOTING OF LOSS OF RIGHTS PURSUANT TO RULE 112(1) EPC (EPO FORM 1205 DATED 20/04/2023)

122 Ep: pct application non-entry in european phase

Ref document number: 20929930

Country of ref document: EP

Kind code of ref document: A1