WO2021195023A3 - Compositions and methods for enhancing detection of rna - Google Patents

Compositions and methods for enhancing detection of rna Download PDF

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Publication number
WO2021195023A3
WO2021195023A3 PCT/US2021/023589 US2021023589W WO2021195023A3 WO 2021195023 A3 WO2021195023 A3 WO 2021195023A3 US 2021023589 W US2021023589 W US 2021023589W WO 2021195023 A3 WO2021195023 A3 WO 2021195023A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
rna
compositions
enhancing detection
target rna
Prior art date
Application number
PCT/US2021/023589
Other languages
French (fr)
Other versions
WO2021195023A2 (en
Inventor
Michael Springer
Original Assignee
President And Fellows Of Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by President And Fellows Of Harvard College filed Critical President And Fellows Of Harvard College
Publication of WO2021195023A2 publication Critical patent/WO2021195023A2/en
Publication of WO2021195023A3 publication Critical patent/WO2021195023A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • AIDS & HIV (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The technology described herein is directed to methods, kits, and systems for detecting a target RNA, such as a small amount of viral RNA. In one aspect, described herein are methods of detecting the target RNA. In other aspects, described herein are kits and systems suitable to practice the methods described herein to detect the target RNA.
PCT/US2021/023589 2020-03-23 2021-03-23 Compositions and methods for enhancing detection of rna WO2021195023A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US202062993521P 2020-03-23 2020-03-23
US62/993,521 2020-03-23
US202063031120P 2020-05-28 2020-05-28
US63/031,120 2020-05-28

Publications (2)

Publication Number Publication Date
WO2021195023A2 WO2021195023A2 (en) 2021-09-30
WO2021195023A3 true WO2021195023A3 (en) 2021-11-04

Family

ID=77892628

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2021/023589 WO2021195023A2 (en) 2020-03-23 2021-03-23 Compositions and methods for enhancing detection of rna

Country Status (1)

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WO (1) WO2021195023A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113913557B (en) * 2021-11-10 2023-08-25 中国人民解放军东部战区疾病预防控制中心 Kit for rapidly detecting Hantavirus SEOV-S4 subtype and detection method thereof
WO2023095018A1 (en) * 2021-11-24 2023-06-01 D-Nome Private Limited Rapid method for room temperature reverse transcription loop-mediated isothermal amplification (rt-lamp) and reagent kit
CN114231664B (en) * 2021-11-30 2023-07-28 华南农业大学 Epinephelus akaara nerve necrosis virus RGNNV detection kit and detection method

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017106790A1 (en) * 2015-12-18 2017-06-22 Clear Gene, Inc. Methods, compositions, kits and devices for rapid analysis of biological markers
US20190256898A1 (en) * 2016-10-17 2019-08-22 Arizona Board Of Regents On Behalf Of Arizona State University Unimolecular aptamer-based sensors for pathogen detection

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017106790A1 (en) * 2015-12-18 2017-06-22 Clear Gene, Inc. Methods, compositions, kits and devices for rapid analysis of biological markers
US20190256898A1 (en) * 2016-10-17 2019-08-22 Arizona Board Of Regents On Behalf Of Arizona State University Unimolecular aptamer-based sensors for pathogen detection

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
QIAN JASON, BOSWELL SARAH A., CHIDLEY CHRISTOPHER, LU ZHI-XIANG, PETTIT MARY E., GAUDIO BENJAMIN L., FAJNZYLBER JESSE M., INGRAM R: "An enhanced isothermal amplification assay for viral detection", NATURE COMMUNICATIONS, vol. 11, no. 1, 1 December 2020 (2020-12-01), XP055870470, DOI: 10.1038/s41467-020-19258-y *

Also Published As

Publication number Publication date
WO2021195023A2 (en) 2021-09-30

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