WO2021147400A1 - 一种微型孔板 - Google Patents

一种微型孔板 Download PDF

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Publication number
WO2021147400A1
WO2021147400A1 PCT/CN2020/122332 CN2020122332W WO2021147400A1 WO 2021147400 A1 WO2021147400 A1 WO 2021147400A1 CN 2020122332 W CN2020122332 W CN 2020122332W WO 2021147400 A1 WO2021147400 A1 WO 2021147400A1
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micro
orifice plate
film
gas
upper substrate
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PCT/CN2020/122332
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English (en)
French (fr)
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张秀莉
邓权峰
罗勇
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苏州大学
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Publication of WO2021147400A1 publication Critical patent/WO2021147400A1/zh

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor

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  • the invention relates to the technical field of biochemical detection, in particular to a micro-orifice plate.
  • the 96-well plate is now a general platform for biomedical laboratories, mainly used for biochemical analysis, such as immunoassay.
  • the 96 plate currently has two main disadvantages: (1) The volume of each well is relatively large, so the sample consumption is relatively large; (2) The liquid exchange operation of the wells requires manual or large-scale automated operation equipment, which is inconvenient and expensive. .
  • the technical problem to be solved by the present invention is to provide a micro-orifice plate, which can realize the programmed and automatic flow of liquid between the holes, thereby realizing automatic biochemical analysis.
  • the present invention provides a micro-orifice plate, which includes an upper substrate, a lower substrate and an intermediate film; the upper substrate is provided with a plurality of micropores, and at least some of the micropores are connected by flow channels. And there is at least one break point in the flow channel;
  • the lower substrate is provided with cavities corresponding to the breakpoints one-to-one, and at least part of the cavities are connected through gas channels; the lower substrate is also provided with a gas interface for connecting an external air pressure controller, and at least Part of the cavity is communicated with the gas interface through a gas channel;
  • the intermediate layer film is sandwiched between the upper substrate and the lower substrate, a plurality of non-connected liquid channels are enclosed between the upper substrate and the intermediate film, and the lower substrate and the intermediate film are enclosed and connected ⁇ gas channel;
  • the pressure in the cavity is controlled by the air pressure controller to separate or contact the intermediate film and the break point, so that the plurality of liquid channels are communicated or disconnected, and the liquid is mutually connected between the plurality of micropores. Circulation.
  • the upper substrate, the intermediate film and the lower substrate are fixed by bolts and nuts, locking structure, self-locking structure, shell, glue or tape.
  • the materials of the intermediate layer film, the upper substrate and the lower substrate are polyethylene, polyvinyl chloride, polystyrene, polycarbonate, polymethyl phthalate, polytetrafluoroethylene, polyoxymethylene, and polystyrene.
  • Ether polyurethane, polyphenylene sulfide, polyamide, polybutylene terephthalate, polyethylene terephthalate, ethylene-vinyl acetate copolymer, acrylonitrile-butadiene Ene-styrene copolymer, polypropylene or polydimethylsiloxane.
  • micropore communicates with the flow channel through a communication hole.
  • the number of the micropores, breakpoints and gas interfaces is 1 to 10,000.
  • breakpoint is integrally formed with the upper substrate, and the gas interface and the lower substrate.
  • volume of the micropores is 1 to 1000 microliters.
  • the micro-orifice plate further includes an adhesive film, and the adhesive film is attached to the surface of the upper substrate.
  • the adhesive film makes the micropores on the upper substrate form a sealed structure for storing reagents.
  • the adhesive film is a waterproof and breathable film, a porous film or a closed film.
  • micro-orifice plate further includes an air pressure chip, and the air pressure chip is used to control the air pressure of an external air pressure controller.
  • the intermediate film and the lower substrate are assembled, they are connected to an external air pressure controller through a gas interface, reagents are filled in the micropores, and a viscous film is attached and sealed.
  • the liquid channels, cavities and break points of the micro-orifice plate form micro valves, and the liquid flow state in the liquid channels is controlled by the micro valves.
  • the opening and closing state of the microvalve is controlled by the pressure of the cavity. When the pressure is negative, the microvalve is opened; when the pressure is positive, the microvalve is closed. Further, the pressure range of the positive pressure or the negative pressure of the micro-orifice plate is between -10000KPa and 10000KPa.
  • the external air pressure controller can control the size of the gas pressure and realize the instantaneous switching of positive pressure and negative pressure.
  • the output of the external air pressure controller is negative pressure
  • the cavity connected to the gas interface through the gas channel is negative pressure, attracting the middle layer film to move downwards.
  • the middle layer film is separated from the break point, and the middle layer film is separated from the lower layer.
  • There is a negative pressure between the substrates the liquid is sucked in, the pressure is balanced, and the liquid channels on both sides of the breakpoint are connected.
  • the microvalve is in the open state. In the same way, when the output of the external air pressure controller is positive pressure, the state of the microvalve is closed.
  • every three or more microvalves can form a micropump; the following uses "front valve”, “middle valve”, and “rear valve” to describe the working principle of the micropump: when the current valve is under negative pressure , The front valve is opened; at this time, the middle valve is supplied with negative pressure, the negative pressure in the middle valve sucks in the liquid, the front valve is supplied with positive pressure, the front valve is closed; the rear valve is supplied with negative pressure, and the rear valve is opened. At this time, The middle valve enters a positive pressure, and the positive pressure squeezes out the liquid so that the liquid can move from the front valve to the rear valve.
  • the pressure in the cavity is controlled by an external air pressure controller to separate or contact the intermediate film and the breakpoint, thereby connecting or disconnecting a plurality of liquid channels to each other, and realizes that the liquid is in multiple The micropores communicate with each other.
  • the micro-orifice plate of the present invention can realize the programmed and automated transmission of liquid between the wells and the automated biochemical analysis by programmatically controlling a plurality of micro-pumps and micro-valves on the micro-orifice plate.
  • FIG. 1 is a schematic structural diagram of a micro-orifice plate according to an embodiment of the present invention
  • FIG. 2 is a schematic diagram of the structure of a micro-orifice plate according to another embodiment of the present invention.
  • Figure 3 is a schematic cross-sectional view of the micro valve on the micro orifice plate (the micro valve is closed);
  • Figure 4 is a schematic cross-sectional view of the micro valve on the micro orifice plate (the micro valve is in an open state);
  • Figure 5 is the working flow of the micro pump on the micro orifice plate
  • the manufacturing technology and use method of the micro-orifice plate are conventional technologies and methods in the field of biochemical analysis.
  • This embodiment provides a micro-orifice plate, which includes an upper substrate 100, a lower substrate 200, and an intermediate film 300.
  • the upper substrate 100 is provided with a plurality of micropores 110 arranged in a 5 ⁇ 3 matrix, and the bottom of each micropore 110 is provided with a communicating hole 120, two adjacent to each other laterally.
  • the communicating holes 120 are connected by a flow channel, and at least one break point 140 is provided in the flow channel.
  • the break point 140 functions to block the flow of liquid.
  • the lower substrate 200 is provided with cavities 230 corresponding to the break points 140 in a one-to-one manner, so that the cavities 230 form a 5 ⁇ 4 matrix arrangement.
  • each row of cavities 230 is provided with a gas interface 210 which communicates with the outermost cavity 230 through a gas channel 220.
  • the gas interface 210 is used to connect an external air pressure controller.
  • the intermediate film 300 is sandwiched between the upper substrate 100 and the lower substrate 200, wherein a plurality of disconnected liquid channels 130 are enclosed between the upper substrate 100 and the intermediate film 300, and between the lower substrate 200 and the intermediate film 300 Enclose a connected gas channel 220. Under the condition that the upper substrate 100 and the lower substrate 200 exert a force, the intermediate film 300 can be closely attached to the upper substrate 100 and the lower substrate 200 without leaking air or water.
  • Figure 2 shows a schematic structural view of a micro-orifice plate according to another embodiment of the present invention.
  • the upper substrate 100 is provided with a plurality of micro-holes 110 arranged in a 3 ⁇ 4 matrix, and each micro-hole 110 is The bottom of the hole is provided with a communication hole 120, two horizontally adjacent communication holes 120 are connected by a flow channel, and the leftmost row of vertically adjacent two communication holes 120 are connected by a flow channel. There are three break points in each flow channel.
  • the lower substrate 200 is provided with cavities 230 corresponding to the breakpoint 140 one-to-one.
  • each row of cavities 230 is provided with a gas interface 210, and the gas interface 210 is connected to it through a gas channel 220.
  • the outermost cavity 230 communicates with each other.
  • each cavity 230 is connected to a gas interface 210 through a gas channel 220.
  • the liquid channel 130, the cavity 230 and the break point 140 of the micro-orifice plate form a "micro valve", and the liquid flow state in the liquid channel 130 can be controlled by the micro valve.
  • the opening and closing state of the microvalve is controlled by the pressure of the cavity 230. When the pressure is negative, the microvalve is opened; when the pressure is positive, the microvalve is closed. Further, the pressure range of the positive pressure or the negative pressure of the micro-orifice plate is between -10000KPa and 10000KPa.
  • the external air pressure controller can control the size of the gas pressure, and realize the instantaneous switching of positive pressure and negative pressure.
  • the output of the external air pressure controller is negative pressure
  • the cavity 230 connected to the gas interface 210 through the gas channel 220 is negative pressure, attracting the intermediate film 300 to move downwards.
  • the intermediate film 300 is separated from the break point 140 , There is a negative pressure between the intermediate film 300 and the lower substrate 200, the liquid is sucked in, the pressure is balanced, and the liquid channels 130 on both sides of the break point 140 are connected.
  • the microvalve is in an open state.
  • the output of the external air pressure controller is positive pressure
  • the microvalve state is closed.
  • every three or more microvalves can form a micropump; the following uses "front valve”, “middle valve”, and “rear valve” to describe the working principle of the micropump:
  • front valve When the front valve is in negative pressure, the front valve opens; at this time, the middle valve is in negative pressure, the negative pressure in the middle valve sucks in the liquid, the front valve is in positive pressure, the front valve is closed; the rear valve is in negative pressure, The rear valve opens.
  • the middle valve is supplied with positive pressure, and the positive pressure squeezes out the liquid, so that the liquid can move from the front valve to the rear valve.
  • the upper substrate 100, the intermediate film 300 and the lower substrate 200 can be fixed by bolts and nuts, locking structure, self-locking structure, shell, glue or tape.
  • the material of the intermediate layer film 300, the upper substrate 100 and the lower substrate 200 may be polyethylene, polyvinyl chloride, polystyrene, polycarbonate, polymethyl phthalate, polytetrafluoroethylene, and polyoxymethylene.
  • the number of the micropores 110, the breakpoints 140 and the gas interfaces 210 can be 1 to 10,000, the volume of the micropores 110 can be 1 to 1000 microliters, and the breakpoints 140 are connected to the upper substrate 100 and the gas interface 210. It is integrally formed with the lower substrate 200. .
  • the micro-orifice plate further includes an adhesive film (not shown), and the adhesive film is attached to the surface of the upper substrate 100.
  • the adhesive film makes the micropores 110 on the upper substrate 100 form a sealed structure for storing reagents.
  • the adhesive film is a waterproof and breathable film, a porous film or a closed film.
  • the micro-well plate of the present invention When the micro-well plate of the present invention is used, after the upper substrate 100, the intermediate film 300, and the lower substrate 200 are assembled, they are connected to the external air pressure controller through the gas interface 210, the reagent is filled in the microwell 110, and the adhesive film is attached to seal it. That's it.
  • the micro-orifice plate of the present invention can realize the programmed and automatic transmission of liquid between the holes and the holes through programmed control of a plurality of micro-pumps and micro-valves on the micro-orifice plate, thereby realizing automatic biochemical analysis.

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Abstract

一种微型孔板，包括上层基板（100）、下层基板（200）和中间层薄膜（300）；上层基板（100）上设有多个微孔（110），至少部分微孔（110）之间通过流道（130）连接（130），且流道（130）中设有至少一个断点（140）；下层基板（200）上设有与断点（140）一一对应的空腔（230），至少部分空腔（230）之间通过气体通道（220）连通；下层基板（200）上还设有气体接口（210），且至少部分空腔（230）通过气体通道（220）与气体接口（210）连通；中间层薄膜（300）夹设于上层基板（100）与下层基板（200）之间，上层基板（100）与中间层薄膜（300）之间围成多个互不连通的液体通道（130），下层基板（200）与中间层薄膜（300）之间围成连通的气体通道（220）。微型孔板，可以实现孔与孔之间液体的程序化、自动化流动，从而实现自动化的生化分析。

Description

一种微型孔板 技术领域
本发明涉及生化检测技术领域，具体涉及一种微型孔板。
背景技术
96孔板现今是生物医学实验室的一种通用平台，主要用于生化分析，譬如免疫分析。96板目前主要有两个缺点：(1)每个孔的体积较大，因此样品消耗量就比较大；(2)孔的换液操作需要依赖人工或者大型自动化操作仪器，不方便也比较昂贵。
针对这两个缺点，我们试图将96孔板的孔缩小，同时将生化分析所有需要用到的试液均预装在96孔板的孔中，然后孔与孔相互联通，通过压力控制孔与孔之间的液体的流动，从而实现自动化的免疫分析。
发明内容
本发明要解决的技术问题是提供一种微型孔板，该微型孔板可以实现孔与孔之间液体的程序化、自动化流动，从而实现自动化的生化分析。
为了解决上述技术问题，本发明提供了一种微型孔板，包括上层基板、下层基板和中间层薄膜；所述上层基板上设有多个微孔，至少部分微孔之间通过流道连接，且所述流道中设有至少一个断点；
所述下层基板上设有与所述断点一一对应的空腔，至少部分空腔之间通过气体通道连通；所述下层基板上还设有用于连接外部气压控制器的气体接口，且至少部分所述空腔通过气体通道与所述气体接口连通；
所述中间层薄膜夹设于上层基板与下层基板之间，所述上层基板与中间层薄膜之间围成多个互不连通的液体通道，所述下层基板与中间层薄膜之间围成连通的气体通道；
通过所述气压控制器控制空腔内的压力，使所述中间层薄膜与断点分离或接触，从而使多个所述液体通道相互连通或者断开，实现液体在多个微孔之间相互流通。
进一步地，所述上层基板、中间层薄膜和下层基板之间通过螺栓螺母、锁紧结构、自锁结构、外壳、胶水或胶带固定。
进一步地，所述中间层薄膜、上层基板和下层基板的材料为聚乙烯、聚氯乙烯、聚苯乙烯、聚碳酸酯、聚甲基苯烯酸酯、聚四氟乙烯、聚甲醛、聚苯醚、聚氨基甲酸乙酯、聚亚苯基硫醚、聚酰胺、聚对苯二甲酸丁酯、聚对苯二甲酸乙二醇酯、乙烯-醋酸乙烯酯共聚合物、丙烯腈-丁二烯-苯乙烯共聚合物、聚丙烯或聚二甲基硅氧烷。
进一步地，所述微孔通过一连通孔与所述流道连通。
进一步地，所述微孔、断点和气体接口的数目为1～10000个。
进一步地，所述断点与上层基板、气体接口与下层基板一体成型。
进一步地，所述微孔的体积为1～1000微升。
进一步地，所述微型孔板还包括粘性薄膜，所述粘性薄膜贴覆于上层基板的表面。所述粘性薄膜使得上层基板上的微孔形成密封结构，用于储存试剂。进一步地，所述粘性薄膜为防水透气膜、多孔膜或者密闭膜。
进一步地，所述微型孔板还包括气压芯片，所述气压芯片用于控制外部气压控制器的气压。
本发明的微型孔板，上层基板、中间层薄膜、下层基板组装完毕后，通过气体接口接入外部气压控制器，微孔中装入试剂，贴上粘性薄膜密封即可。
本发明中，所述微型孔板的液体通道、空腔与断点形成微阀，通过所述微阀来控制液体通道中的液体流动状态。微阀的开合状态由空腔的压力控制，当压力为负压时，微阀开启；当压力为正压时，微阀关闭。进一步地，所述微型孔板正压或者负压的压力范围在-10000KPa到10000KPa之间。
本发明中，外部气压控制器可以控制气体压力的大小，实现正压和负压的瞬间切换。当外部气压控制器输出为负压时，通过气体通道与气体接口相接的空腔为负压，吸引中间层薄膜向下运动，此时，中间层薄膜与断点分离，中间层薄膜与下层基板之间为负压，液体吸入，压力平衡，断点两侧液体通道连通，此时，微阀状态为开启状态。同理，当外部气压控制器输出为正压时微阀状态为关闭状态。
本发明中，每三个以及三个以上的微阀可以组成一个微泵；以下以“前阀”、“中阀”、“后阀”描述微泵的工作原理：当前阀通入负压时，前阀打开；这时，中阀通入负压，中阀中的负压将液体吸入，前阀通入正压，前阀关闭；后阀通入负压，后阀打开，这时，中阀通入正压，正压将液体挤出，这样液体就可以从前阀移动到后阀。
本发明的有益效果：
1.本发明的微型孔板，通过外部气压控制器控制空腔内的压力，使中间层薄膜与断点分离或接触，从而使多个液体通道相互连通或者断开，实现了液体在多个微孔之间相互流通。
2.本发明的微型孔板，通过程序化控制微型孔板上的多个微泵和微阀，就可以实现孔与孔之间液体程序化和自动化的传输，从而实现自动化生化分析。
附图说明
图1是本发明一实施例的微型孔板的结构示意图；
图2是本发明另一实施例的微型孔板的结构示意图；
图3是微型孔板上微阀的截面示意图(微阀关闭状态)；
图4是微型孔板上微阀的截面示意图(微阀开启状态)；
图5是微型孔板上微泵的工作流程；
图中标号说明：100、上层基板；110、微孔；120、连通孔；130、液体通道；140、断点；200、下层基板；210、气体接口；220、气体通道；230、空腔；300、中间层薄膜；
A到G是阀门开关顺序；H是微泵的俯视图。
具体实施方式
下面结合附图和具体实施例对本发明作进一步说明，以使本领域的技术人员可以更好地理解本发明并能予以实施，但所举实施例不作为对本发明的限定。
下述实施例中所使用的实验方法如无特殊说明，均为常规方法。
下述实施例中所用的材料、试剂等，如无特殊说明，均可从商业途径得到。
下述实施例中，微型孔板制作技术和使用方法均为生化分析领域的常规技术和方法。
以下实施例中，术语“横向”、“纵向”、“左侧”等仅相对于附图1-2中的方位所说，并不代表绝对方位。
实施例1
本实施例提供了一种微型孔板，包括上层基板100、下层基板200和中间层薄膜300。
参照图1，3-4所示，上层基板100上设有按5×3矩阵式排列的多个微孔110，每个微孔110的孔底均设有一连通孔120，横向相邻的两连通孔120之间通过流道连接，且所述流道中设有至少一个断点140。本实施例中，横向的两流道中各设有1和3个断点140。该断点140起到了阻断液体流动的功能。
下层基板200上设有与所述断点140一一对应的空腔230，从而所述空腔230形成了5×4的矩阵式排列。纵向排列的4列空腔230中，每列空腔230均设有一气体接口210，该气体接口210通过气体通道220与其中最外侧的一空腔230连通。所述气体接口210用于连接外部的气压控制器。
中间层薄膜300夹设于上层基板100与下层基板200之间，其中上层基板100与中间层薄膜300之间围成多个互不连通的液体通道130，下层基板200与中间层薄膜300之间围成连通的气体通道220。在上层基板100和下层基板200施加作用力的条件下，中间层薄膜300能够与上层基板100与下层基板200紧密贴合，不漏气、不漏水。
实施例2
图2示出了本发明另一实施例的微型孔板的结构示意图，该实施例中，上层基板100上设有按3×4矩阵式排列的多个微孔110，每个微孔110的孔底均设有一连通孔120，横向相邻的两连通孔120之间通过流道连接，且最左侧的一列竖向相邻的两连通孔120之间通过流道连接。每个流道中均设有三个断点。
下层基板200上设有与断点140一一对应的空腔230，纵向排列的9列空腔230中，每列空腔230均设有一气体接口210，该气体接口210通过气体通道220与其中最外侧的一空腔230连通。最左侧的一列空腔230中，每个空腔230均通过气体通道220与一气体接口210连通。
本发明中，微型孔板的液体通道130、空腔230与断点140形成“微阀”，通过微阀即可控制液体通道130中的液体流动状态。微阀的开合状态由空腔230 的压力控制，当压力为负压时，微阀开启；当压力为正压时，微阀关闭。进一步地，所述微型孔板正压或者负压的压力范围在-10000KPa到10000KPa之间。
本发明中，外部的气压控制器可以控制气体压力的大小，实现正压和负压的瞬间切换。当外部气压控制器输出为负压时，通过气体通道220与气体接口210相接的空腔230为负压，吸引中间层薄膜300向下运动，此时，中间层薄膜300与断点140分离，中间层薄膜300与下层基板200之间为负压，液体吸入，压力平衡，断点140两侧液体通道130连通，此时，微阀状态为开启状态。同理，当外部气压控制器输出为正压时微阀状态为关闭状态。
参见图3-5，本实施例中，每三个以及三个以上的微阀可以组成一个微泵；以下以“前阀”、“中阀”、“后阀”描述微泵的工作原理：当前阀通入负压时，前阀打开；这时，中阀通入负压，中阀中的负压将液体吸入，前阀通入正压，前阀关闭；后阀通入负压，后阀打开，这时，中阀通入正压，正压将液体挤出，这样液体就可以从前阀移动到后阀。
本发明中，上层基板100、中间层薄膜300和下层基板200之间可通过螺栓螺母、锁紧结构、自锁结构、外壳、胶水或胶带等方式固定。
本发明中，中间层薄膜300、上层基板100和下层基板200的材料可为聚乙烯、聚氯乙烯、聚苯乙烯、聚碳酸酯、聚甲基苯烯酸酯、聚四氟乙烯、聚甲醛、聚苯醚、聚氨基甲酸乙酯、聚亚苯基硫醚、聚酰胺、聚对苯二甲酸丁酯、聚对苯二甲酸乙二醇酯、乙烯-醋酸乙烯酯共聚合物、丙烯腈-丁二烯-苯乙烯共聚合物、聚丙烯或聚二甲基硅氧烷。
本发明中，所述微孔110、断点140和气体接口210的数目可为1～10000个，微孔110的体积可为1～1000微升，断点140与上层基板100、气体接口210与下层基板200一体成型。。
本发明中，微型孔板还包括粘性薄膜(图未示)，所述粘性薄膜贴覆于上 层基板100的表面。所述粘性薄膜使得上层基板100上的微孔110形成密封结构，用于储存试剂。进一步地，所述粘性薄膜为防水透气膜、多孔膜或者密闭膜。
本发明的微型孔板使用时，将上层基板100、中间层薄膜300、下层基板200组装完毕后，通过气体接口210接入外部气压控制器，微孔110中装入试剂，贴上粘性薄膜密封即可。
本发明的微型孔板，通过程序化控制微型孔板上的多个微泵和微阀，就可以实现孔与孔之间液体程序化和自动化的传输，从而实现自动化生化分析。
以上所述实施例仅是为充分说明本发明而所举的较佳的实施例，本发明的保护范围不限于此。本技术领域的技术人员在本发明基础上所作的等同替代或变换，均在本发明的保护范围之内。本发明的保护范围以权利要求书为准。

Claims (10)

  1. 一种微型孔板，其特征在于，包括上层基板、下层基板和中间层薄膜；所述上层基板上设有多个微孔，至少部分微孔之间通过流道连接，且所述流道中设有至少一个断点；
    所述下层基板上设有与所述断点一一对应的空腔，至少部分空腔之间通过气体通道连通；所述下层基板上还设有用于连接外部气压控制器的气体接口，且至少部分所述空腔通过气体通道与所述气体接口连通；
    所述中间层薄膜夹设于上层基板与下层基板之间，所述上层基板与中间层薄膜之间围成多个互不连通的液体通道，所述下层基板与中间层薄膜之间围成连通的气体通道；
    通过外部气压控制器控制空腔内的压力，使所述中间层薄膜与断点分离或接触，从而使多个所述液体通道相互连通或者断开，实现液体在多个微孔之间相互流通。
  2. 如权利要求1所述的微型孔板，其特征在于，所述上层基板、中间层薄膜和下层基板之间通过螺栓螺母、锁紧结构、自锁结构、外壳、胶水或胶带固定。
  3. 如权利要求1所述的微型孔板，其特征在于，所述中间层薄膜、上层基板和下层基板的材料为聚乙烯、聚氯乙烯、聚苯乙烯、聚碳酸酯、聚甲基苯烯酸酯、聚四氟乙烯、聚甲醛、聚苯醚、聚氨基甲酸乙酯、聚亚苯基硫醚、聚酰胺、聚对苯二甲酸丁酯、聚对苯二甲酸乙二醇酯、乙烯-醋酸乙烯酯共聚合物、丙烯腈-丁二烯-苯乙烯共聚合物、聚丙烯和聚二甲基硅氧烷中的一种。
  4. 如权利要求1所述的微型孔板，其特征在于，所述微孔通过一连通孔与所述流道连通。
  5. 如权利要求1所述的微型孔板，其特征在于，所述微孔、断点和气体接口的数目为1～10000个。
  6. 如权利要求1所述的微型孔板，其特征在于，所述断点与上层基板、气体接口与下层基板一体成型。
  7. 如权利要求1所述的微型孔板，其特征在于，所述微孔的体积为1～1000微升。
  8. 如权利要求1所述的微型孔板，其特征在于，所述微型孔板还包括粘性薄膜，所述粘性薄膜贴覆于上层基板的表面。
  9. 如权利要求8所述的微型孔板，其特征在于，所述粘性薄膜为防水透气膜、多孔膜或者密闭膜。
  10. 如权利要求1所述的微型孔板，其特征在于，所述微型孔板还包括气压芯片，所述气压芯片用于控制外部气压控制器的气体压力。
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