WO2021128036A1 - Kit permettant de construire une bibliothèque de séquençage de tcr monocellulaire humain et son utilisation - Google Patents
Kit permettant de construire une bibliothèque de séquençage de tcr monocellulaire humain et son utilisation Download PDFInfo
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- WO2021128036A1 WO2021128036A1 PCT/CN2019/128162 CN2019128162W WO2021128036A1 WO 2021128036 A1 WO2021128036 A1 WO 2021128036A1 CN 2019128162 W CN2019128162 W CN 2019128162W WO 2021128036 A1 WO2021128036 A1 WO 2021128036A1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
- C40B50/18—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support using a particular method of attachment to the solid support
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- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
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- Molecular Biology (AREA)
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- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
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- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Structural Engineering (AREA)
- Medicinal Chemistry (AREA)
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- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
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Abstract
L'invention concerne un kit permettant de construire une bibliothèque de séquençage de TCR monocellulaire humain et son utilisation. L'invention concerne également une méthode basée sur un kit destinée à construire une bibliothèque de séquençage de TCR monocellulaire humain, comprenant : l'utilisation d'une puce microfluidique pour effectuer une étape de capture et d'encapsulation de cellules isolées, puis la réalisation d'étapes telles qu'une transcription inverse d'ARN, une pré-amplification d'ADNc, une première amplification, une seconde amplification, une fragmentation, une amplification et une purification. Lorsque le kit et la bibliothèque de séquençage de TCR monocellulaire humain sont appliqués au séquençage du répertoire immunitaire de cellules immunitaires, de 500 à 30000 cellules peuvent être séparées en une seule expérience.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2019/128162 WO2021128036A1 (fr) | 2019-12-25 | 2019-12-25 | Kit permettant de construire une bibliothèque de séquençage de tcr monocellulaire humain et son utilisation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2019/128162 WO2021128036A1 (fr) | 2019-12-25 | 2019-12-25 | Kit permettant de construire une bibliothèque de séquençage de tcr monocellulaire humain et son utilisation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2021128036A1 true WO2021128036A1 (fr) | 2021-07-01 |
Family
ID=76575050
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/CN2019/128162 WO2021128036A1 (fr) | 2019-12-25 | 2019-12-25 | Kit permettant de construire une bibliothèque de séquençage de tcr monocellulaire humain et son utilisation |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO2021128036A1 (fr) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107747134A (zh) * | 2017-10-20 | 2018-03-02 | 重庆天科雅生物科技有限公司 | 一种构建人TCRalphaCDR3区文库的方法 |
| CN107893068A (zh) * | 2017-10-20 | 2018-04-10 | 重庆天科雅生物科技有限公司 | 一种构建人TCRbetaCDR3区文库的方法 |
| CN108473984A (zh) * | 2015-11-04 | 2018-08-31 | 阿特雷卡公司 | 用于分析与单个细胞相关联的核酸的核酸条形码的组合组 |
| CN109851711A (zh) * | 2018-12-29 | 2019-06-07 | 苏州绘真生物科技有限公司 | 可溶性水凝胶微球及其制备方法与在单细胞检测中的应用 |
-
2019
- 2019-12-25 WO PCT/CN2019/128162 patent/WO2021128036A1/fr active Application Filing
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108473984A (zh) * | 2015-11-04 | 2018-08-31 | 阿特雷卡公司 | 用于分析与单个细胞相关联的核酸的核酸条形码的组合组 |
| CN107747134A (zh) * | 2017-10-20 | 2018-03-02 | 重庆天科雅生物科技有限公司 | 一种构建人TCRalphaCDR3区文库的方法 |
| CN107893068A (zh) * | 2017-10-20 | 2018-04-10 | 重庆天科雅生物科技有限公司 | 一种构建人TCRbetaCDR3区文库的方法 |
| CN109851711A (zh) * | 2018-12-29 | 2019-06-07 | 苏州绘真生物科技有限公司 | 可溶性水凝胶微球及其制备方法与在单细胞检测中的应用 |
Non-Patent Citations (3)
| Title |
|---|
| AUDE I. SEGALINY, GUIDENG LI, LINGSHUN KONG, CI REN, XIAOMING CHEN, JESSICA K. WANG, DAVID BALTIMORE, GUIKAI WU, WEIAN ZHAO: "Functional TCR T cell screening using single-cell droplet microfluidics", LAB ON A CHIP, ROYAL SOCIETY OF CHEMISTRY, UK, vol. 18, no. 24, 4 December 2018 (2018-12-04), UK, pages 3733 - 3749, XP055578872, ISSN: 1473-0197, DOI: 10.1039/C8LC00818C * |
| MARCO DE SIMONE, GRAZISA ROSSETTI, MASSIMILIANO PAGANI: "Single Cell T Cell Receptor Sequencing: Techniques and Future Challenges", FRONTIERS IN IMMUNOLOGY, vol. 9, XP055698378, DOI: 10.3389/fimmu.2018.01638 * |
| SATPATHY ANSUMAN T.; SALIGRAMA NARESHA; BUENROSTRO JASON D.; WEI YUNING; WU BEIJING; RUBIN ADAM J.; GRANJA JEFFREY M.; LAREAU CALE: "Transcript-indexed ATAC-seq for precision immune profiling", NATURE MEDICINE, NATURE PUB. CO, NEW YORK, vol. 24, no. 5, 23 April 2018 (2018-04-23), New York, pages 580 - 590, XP036901064, ISSN: 1078-8956, DOI: 10.1038/s41591-018-0008-8 * |
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