WO2021122770A1 - Procédé de réduction de la propension à l'auto-échauffement d'un agpi-lc microbien comportant une biomasse - Google Patents

Procédé de réduction de la propension à l'auto-échauffement d'un agpi-lc microbien comportant une biomasse Download PDF

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WO2021122770A1
WO2021122770A1 PCT/EP2020/086455 EP2020086455W WO2021122770A1 WO 2021122770 A1 WO2021122770 A1 WO 2021122770A1 EP 2020086455 W EP2020086455 W EP 2020086455W WO 2021122770 A1 WO2021122770 A1 WO 2021122770A1
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microbial cells
composition
vegetable oil
heating
self
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PCT/EP2020/086455
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English (en)
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Martin Heining
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Dsm Ip Assets B.V.
Evonik Operations Gmbh
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Publication of WO2021122770A1 publication Critical patent/WO2021122770A1/fr

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/115—Fatty acids or derivatives thereof; Fats or oils
    • A23L33/12—Fatty acids or derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
    • A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]

Definitions

  • the present invention relates to a method of reducing the self-heating propensity of a microbial biomass which contains significant amount of polyunsaturated fatty acids.
  • Polyunsaturated fatty acids (PUFAs) containing lipids are of high interest in the feed, food and pharmaceutical industry.
  • Fatty acids are classified based on the length and saturation characteristics of the carbon chain.
  • Fatty acids are termed short chain, medium chain, or long chain fatty acids based on the number of carbons present in the chain.
  • Fatty acids are termed saturated fatty acids when no double bonds are present between the carbon atoms.
  • Fatty acids are termed unsaturated fatty acids when double bonds are present.
  • Unsaturated long chain fatty acids are monounsaturated when only one double bond is present.
  • Unsaturated long chain fatty acids are polyunsaturated when more than one double bond is present.
  • PUFAs can be produced by microorganisms in a fermentation process.
  • the biomass of the PUFA-containing microorganism is collected before being processed to extract the PUFA oil contained within.
  • the biomass of the PUFA-containing microorganism can also be used directly as a product, particularly in the feed industry.
  • PUFA-containing compositions are susceptible to self heating. For example, during storage or transportation, the temperature of the biomass in the container or package can increase spontaneously, some will ultimately result in unexpected explosions and fires.
  • a composition comprising more than 30 wt% vegetable oil and at least 50 wt% microbial cells, wherein said microbial cells comprise one or more type of polyunsaturated fatty acid (PUFA) having at least 20 carbon atoms and at least three double bonds, wherein the microbial cells has at least 20 wt% PUFAs, and wherein the composition’s self-heating onset temperature is at least 250 °C.
  • the microbial cells comprise at least 30 wt% PUFAs.
  • the microbial cells comprise at least 40 wt% PUFAs.
  • the microbial cells comprise at least 50 wt% PUFAs.
  • the PUFA is an w-3 or an w-6 PUFA.
  • the microbial cells are ruptured.
  • composition comprises more than 40 wt% vegetable oil.
  • the composition comprises more than 30 wt% vegetable oil and remainder wt% microbial cells.
  • the vegetable oil is canola oil.
  • the microbial cells are of the genus Mortierella , Schizochytrium , Thraustochytrium , Aurantiochytrium , or Crypthecodinium.
  • the onset of composition’s self-heating is measured by heating the composition packed in a 6cm 3 sample tube in a Grewe-Oven with a heating rate of lK/min and an airflow of 2L/min.
  • the microbial cells are in the form of a biomass.
  • the microbial cells are a single strain of microorganism.
  • the composition’s self-heating onset temperature is at least 270 °C.
  • the composition’s self-heating onset temperature is at least 290 °C.
  • the composition’s self-heating onset temperature is at least 300 °C.
  • the microbial cells are of the genus Mortierella, Schizochytrium , Thraustochytrium , Aurantiochytrium , or Crypthecodinium.
  • the onset of composition’s self-heating is measured by heating the composition packed in a 6cm 3 sample tube in a Grewe- Oven with a heating rate of lK/min and an airflow of 2L/min.
  • the microbial cells are in the form of a biomass.
  • the microbial cells are a single strain of microorganism.
  • the composition’s self-heating onset temperature is at least 270 °C.
  • the composition’s self-heating onset temperature is at least 290 °C.
  • the composition’s self-heating onset temperature is at least 300 °C.
  • the patent or application file contains at least one drawing executed in color.
  • Fig. 1 shows the flowability of four compositions which are made of a mixture of ruptured microbial cell biomass and vegetable oil at different ratios, ranging from 30%:70% to 70%:30% (biomass: vegetable oil).
  • Fig. 2 shows the temperature change curves which were measured over time both inside the 6 cm 2 testing vessel and in the Grewe-oven and the onset temperature of the first self heating event.
  • the testing vessel contains 50% microbial cell biomass and 50% vegetable oil.
  • Fig. 6 shows the temperature change curves which were measured over time both inside the 6 cm 2 testing vessel and in the Grewe-oven and the onset temperature of the first self- heating event.
  • the testing vessel contains 30% microbial cell biomass and 70% vegetable oil.
  • Fig. 7 shows the temperature change curves which were measured over time both inside the 6 cm 2 testing vessel and in the Grewe-oven and the onset temperature of the first self- heating event.
  • the testing vessel contains 100% vegetable oil.
  • Fig. 8 shows the changes of the onset temperature of the first self-heating event in connection with the changes of the wt% of microbial cell biomass.
  • Dried PUFA-containing oleaginous biomass is known to undergo oxidization thus is susceptible to spontaneous self-heating.
  • Such self-heating problem is especially significant in microbial cells which contains long chain polyunsaturated fatty acids (LC-PUFA).
  • LC-PUFA long chain polyunsaturated fatty acids
  • the higher PUFA content there is in a composition the more likely that the composition is susceptible to spontaneous self-heating.
  • PUFAs with 20 or more carbon atoms have higher susceptibility to self-heating.
  • the susceptibility of a biomass increases with higher number of double bonds of the PUFAs.
  • PUFAs with 3 or more double bonds have higher susceptibility to self-heating.
  • One indicator of the self-heating propensity of a composition is the onset temperature for a spontaneous temperature increase to occur when the composition is heated up at a linear rate.
  • a sudden increase (“spike”) of temperature indicates that the composition heats up spontaneously by itself instead of by the external heating source.
  • the higher of this onset temperature is for a composition, the less susceptible that the composition is to self-heating.
  • the lower of this onset temperature is for a composition, the more susceptible that the composition is to self-heating. Any process which can significantly increase the onset temperature of a composition and thus cause self-heating to occur at a higher temperature is considered an effective method for reducing the risk of self-heating,
  • the onset temperature for self-heating of a PUFA-containing oleaginous biomass can be rendered significantly higher by rupturing the biomass and mixing the resulting cell debris/ PUFA oil combination with at least 40 wt% vegetable oil.
  • the resulting composition has a self-heating onset temperature which is about 140 °C higher than onset temperature of 150 °C that is normally observed in the same biomass before the treatment.
  • the self-heating onset temperature is determined by a test described in VDI-
  • a glass vessel with a 6 cm 2 volume is used.
  • the sample size is 100% of the volume of the vessel.
  • the vessel is closed with a rubber stopper, tightened to prevent air intake.
  • a thermocouple is inserted in the vessel through a hole in the center of the stopper.
  • the vessel containing the sample, which has an initial temperature of 20 °C, is placed in a Grewe oven.
  • a thermocouple is placed in the oven for monitoring the temperature increase within the oven.
  • the oven is heated in a way to maintain a heating rate of lK/min with an airflow rate of 2L/min. The heating is stopped when the oven temperature reaches 450 °C.
  • the microbial cells according to the invention have an oil content and PUFA as described below.
  • the microbial cells of the invention have self-heating propensity before treatment because it contains a reasonable level of polyunsaturated fatty acids.
  • the microbial cells comprise between 20-50 wt.%, 20-30 wt.%, 20-40 wt.%, 20-50 wt.%, 20-60 wt.%, between 30-70 wt.%, between 40-60 wt.%, or between 45-55 wt.% PUFAs.
  • the weight of the microbial cells is referred to as the dry cell weight of a biomass. Such biomass can be algal cells or any other PUFA-containing microbial cells.
  • the composition comprises PUFA, specially
  • the composition comprises a biomass. In another embodiment, the composition comprises a dried biomass. In another embodiment, the composition comprises the dried biomass of microbial cells. In another embodiment, the composition comprises the dried biomass of algal cells. In one embodiment, the microbial cells or algal cells are ruptured. The cells are considered ruptured when the oil contained in the cells, such as PUFA oil, is released from the cells.
  • the composition comprises at least 35 wt% vegetable oil and at least 50 wt% microbial cells. In an embodiment of the invention, the composition comprises at least 40 wt% vegetable oil and at least 50 wt% microbial cells. In another embodiment, the composition comprises between 30 wt% to 90 wt% vegetable oil and between 10 wt% and 70 wt% microbial cells. In another embodiment, the composition comprises between 40 wt% to 90 wt% vegetable oil and between 10 wt% and 60 wt% microbial cells. In another embodiment, the composition comprises between 30 wt% to 80 wt% vegetable oil and between 20 wt% and 70 wt% microbial cells.
  • the composition comprises between 40 wt% to 70 wt% vegetable oil and between 30 wt% and 60 wt% microbial cells. In another embodiment, the composition comprises between 40 wt% to 60 wt% vegetable oil and between 40 wt% and 60 wt% microbial cells. In another embodiment, the composition comprises between 40 wt% to 50 wt% vegetable oil and between 50 wt% and 60 wt% microbial cells. In another embodiment, the composition comprises between at least 30 wt% vegetable oil and the remainder wt% microbial cells. In another embodiment, the composition comprises between at least 35 wt% vegetable oil and the remainder wt% microbial cells.
  • the composition comprises between at least 40 wt% vegetable oil and the remainder wt% microbial cells.
  • the composition comprises between 40 wt% vegetable oil and the 60 wt% microbial cells, or comprises between 35 wt% vegetable oil and the 65 wt% microbial cells, or comprises between 45 wt% vegetable oil and the 55 wt% microbial cells, or comprises between 45 wt% vegetable oil and the 55 wt% microbial cells, or comprises between 50 wt% vegetable oil and the 50 wt% microbial cells, or comprises between 60 wt% vegetable oil and the 40 wt% microbial cells, or comprises between 70 wt% vegetable oil and the 30 wt% microbial cells, or comprises between 80 wt% vegetable oil and the 20 wt% microbial cells, or comprises between 90 wt% vegetable oil and the 10 wt% microbial cells.
  • the invention is also directed to a method for increasing the onset self-heating temperature of a composition which comprises microbial cells that is rich in LC-PUFA. It is surprisingly found in this invention that by mixing vegetable oil with LC-PUFA containing microbial cells, the onset self-heating temperature of the microbial cells is rendered significantly higher than before the mixing. The onset temperature increases when more than 30 wt% vegetable oil is included in the microbial cells/vegetable oil blend. In one embodiment, unruptured microbial cells are mixed with more than 30% vegetable oil. In another embodiment, the microbial cells is ruptured to release the PUFA oils it contains and thus together with the added vegetable oil to create a homogenized, fluid form of mixture.
  • the invention is directed to a method for increasing the self heating onset temperature of a composition to at least 250 °C, wherein the composition comprises more than 30 wt% vegetable oil and at least 50 wt% microbial cells, wherein said microbial cells comprise one or more polyunsaturated fatty acid (PUFA) having at least 20 carbon atoms and at least three double bonds, wherein the microbial cells has at least 20 wt% PUFAs, and therein the method comprises mixing the microbial cells with the vegetable oil and rupturing the microbial cells.
  • the composition comprises more than 35 wt% vegetable oil and at least 50 wt% microbial cells.
  • the composition comprises more than 40 wt% vegetable oil and at least 50 wt% microbial cells.
  • the microbial cells recited in the method above comprise at least 20 wt.%, for instance at least 25 wt.%, for instance at least 30 wt.%, for instance at least 35 wt.%, for instance at least 40 wt.%, for instance at least 45 wt.%, for instance at least 50 wt.%, for instance at least 55 wt.%, for instance at least 60 wt.%, for instance at least 65 wt.%, for instance at least 70 wt.%, for instance at least 75 wt.%, for instance at least 80 wt.%, for instance at least 90 wt.%, for instance at least 95 wt.% PUFA.
  • the composition recited in the method described above comprises at least 35 wt% vegetable oil and at least 50 wt% microbial cells.
  • the composition comprises at least 40 wt% vegetable oil and at least 50 wt% microbial cells.
  • the composition comprises between 30 wt% to 90 wt% vegetable oil and between 10 wt% and 70 wt% microbial cells.
  • the composition comprises between 40 wt% to 90 wt% vegetable oil and between 10 wt% and 60 wt% microbial cells.
  • the composition comprises between 30 wt% to 80 wt% vegetable oil and between 20 wt% and 70 wt% microbial cells. In another embodiment, the composition comprises between 30 wt% to 70 wt% vegetable oil and between 30 wt% and 70 wt% microbial cells. In another embodiment, the composition comprises between 30 wt% to 60 wt% vegetable oil and between 40 wt% and 70 wt% microbial cells. In another embodiment, the composition comprises between 30 wt% to 50 wt% vegetable oil and between 50 wt% and 70 wt% microbial cells.
  • the composition comprises between 40 wt% to 80 wt% vegetable oil and between 20 wt% and 60 wt% microbial cells. In another embodiment, the composition comprises between 40 wt% to 70 wt% vegetable oil and between 30 wt% and 60 wt% microbial cells. In another embodiment, the composition comprises between 40 wt% to 60 wt% vegetable oil and between 40 wt% and 60 wt% microbial cells. In another embodiment, the composition comprises between 40 wt% to 50 wt% vegetable oil and between 50 wt% and 60 wt% microbial cells. In another embodiment, the composition comprises between at least 30 wt% vegetable oil and the remainder wt% microbial cells.
  • the composition comprises between at least 35 wt% vegetable oil and the remainder wt% microbial cells. In another embodiment, the composition comprises between at least 40 wt% vegetable oil and the remainder wt% microbial cells. In some specific embodiments, the composition comprises between 40 wt% vegetable oil and the 60 wt% microbial cells, or comprises between 35 wt% vegetable oil and the 65 wt% microbial cells, or comprises between 45 wt% vegetable oil and the 55 wt% microbial cells, or comprises between 45 wt% vegetable oil and the 55 wt% microbial cells, or comprises between 50 wt% vegetable oil and the 50 wt% microbial cells, or comprises between 60 wt% vegetable oil and the 40 wt% microbial cells, or comprises between 70 wt% vegetable oil and the 30 wt% microbial cells, or comprises between 80 wt% vegetable oil and the 20 wt% microbial cells, or comprises between 90 wt% vegetable oil and the 10
  • the microbial cells are ruptured. In another embodiment, the microbial cells are unruptured. In another embodiment, the microbial cells biomass.
  • the microbial cells may be of the genus Mortierella , Schizochytrium , Thraustochytrium , Aurantiochytrium , or Crypthecodinium .
  • the above described PUFAs is an w-3 or an w-6 PUFA.
  • the above described PUFAs is one or more PUFA selected from selected from dihomo-y-linolenic acid (DGLA, 20:3 w-6), arachidonic acid (ARA, 20:4 w-6), eicosapentaenoic acid (EPA, 20:5 w-3), docosahexaenoic acid (DHA: 22:6 w-3), docosapentaenoic acid (DPA 22:5 w-3, or DPA 22:5, w-6).
  • DGLA dihomo-y-linolenic acid
  • ARA arachidonic acid
  • EPA eicosapentaenoic acid
  • DHA docosahexaenoic acid
  • DPA 22:5 w-3 docosapentaenoic acid
  • LC-PUFAs described in this application are fatty acids that contain at least 3 double bonds and have a chain length of 20 or more carbons.
  • Polyunsaturated fatty acids (PUFAs) are classified based on the position of the first double bond from the methyl end of the fatty acid; omega-3 (n-3) fatty acids contain a first double bond at the third carbon, while omega-6 (n-6) fatty acids contain a first double bond at the sixth carbon.
  • a "cell” refers to an oil-containing biomaterial, such as biomaterial derived from oleaginous microorganisms. Oil produced by a microorganism or obtained from a microbial cell is referred to as "microbial oil”. In one embodiment, microbial oil refers to a crude oil extracted from the biomass of the microorganism with or without further processing. Oil produced by algae and/or fungi is also referred to as algal and/or fungal oil, respectively.
  • a "microorganism” refers to organisms such as algae, bacteria, fungi, yeast, protist, and combinations thereof, e.g., unicellular organisms.
  • a microbial cell is a eukaryotic cell.
  • a microbial cell includes, but is not limited to, golden algae (e.g., microorganisms of the kingdom Stramenopiles ); green algae; diatoms; dinoflagellates (e.g., microorganisms of the order Dinophyceae including members of the genus Crypthecodinium such as, for example, Crypthecodinium cohnii or C.
  • cohnii microalgae of the order Thraustochytriales
  • yeast Ascomycetes or Basidiomycetes
  • fungi of the genera Mucor Mortierella, including but not limited to Mortierella alpina and Mortierella sect , schmuckeri , and Pythium , including but not limited to Pythium insidiosum.
  • the microorganisms are from the genus Mortierella , genus
  • the microbial cells are from Crypthecodinium cohnii. In yet an even further embodiment, the microbial cells are selected from Crypthecodinium cohnii , Mortierella alpina , genus Thraustochytrium , genus Schizochytrium , and mixtures thereof.
  • ARA is obtained from microbial cells from the genus Mortierella , which includes, but is not limited to, Mortierella elongata, Mortierella exigua, Mortierella hygrophila , Mortierella alpina , Mortierella schmuckeri , and Mortierella minutissima.
  • the microbial cells are from Mortierella alpina.
  • the microbial cells are from microalgae of the order
  • Thraustochytriales which includes, but is not limited to, the genera Thraustochytrium (species include arudimentale , aureum , benthicola , globosum, kinnei , motivum , multirudimentale , pachydermum , proliferum , roseum, striatum ); the genera Schizochytrium (species include aggregation, limnaceum , mangrovei, minutum , octosporum ); the genera Ulkenia (species include amoeboidea , kerguelensis , minuta , profunda , radiate , sailens , sarkariana, schizochytrops , visurgensis, yorkensis ); the genera Aurantiacochytrium the genera ()blongichytrium the genera Sicyoidochytiunr, the genera Parientichytrium ; the genera Botryochytrium ; and combinations
  • the microbial cells are from the order Thraustochytriales. In yet another embodiment, the microbial cells are from Thraustochytrium. In still a further embodiment, the microbial cells are from Schizochytrium. In a still further embodiment, the microbial cells are chosen from genus Mortierella , Schizochytrium , Thraustochytrium , Aurantiochytrium , Crypthecodinium , or mixtures thereof.
  • the vegetable oil used in the present invention can be any vegetable oil or a blend of different vegetable oils.
  • the vegetable oil is canola oil.
  • the vegetable oil is selected from a group consisting of canola oil, soybean oil, sunflower seed oil, peanut oil, flaxseed oil, sesame seed oil, corn oil, or a combination of the above.
  • sample mixtures A through G containing different weight ratio of microbial biomass and vegetable oil were made. See Table 1.
  • the microbial biomass used in this experiment was Schizochytrium strain No. ATCC-20888. It was purchased from DSM Nutritional Products LLC. The biomass is also called DHAgold ® .
  • the total amount of long chain polyunsaturated fatty acids (PUFA) which have at least 20 carbon atoms and at least three double bonds in the microbial biomass is about 32 wt% of the biomass.
  • the vegetable oil is a food grade Kroger brand pure canola oil purchased from a supermarket. Table 1
  • Example 1 was measured and compared. Specifically, the onset temperature of self-heating for the different mixtures were measured.
  • the onset temperature of self-heating was measured using a Gewer Oven test.
  • the sample mixtures which were prepared in Example 1 were filled into a 6cm 3 test tube.
  • the oven was heated at a rate such that the oven temperature increased linearly at a rate of lK/min
  • the airflow of the oven was set to 2L/min.
  • the test protocol described in VDI-Guideline 2263 was followed.
  • the onset temperature of self-heating is the first temperature at which the sample heats up faster inside the test vessel than the pre-heated air in the oven. Such “spike”, like the one shown Fig. 2 at the time point of a little over 2 hours, indicated the first occurrence of spontaneous self-heating of the material in the test tube.
  • the onset temperature of the first self-heating event of mixtures A, B, D, E, F, and G was measured and is shown in Table 2.
  • the onset temperature of self-heating was maintained at about 150 °C.
  • the DHAgold ® microbial biomass was lowered to 70% and the canola oil was increased to more than 30%, the onset temperature of self-heating increased about 150 °C to about 300 °C. Further increasing the wt% of canola oil and decreasing the wt% of DHAgold ® microbial biomass did not significantly change the onset temperature.

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Abstract

La présente invention concerne une composition de biomasse AGPI-LC qui présente une propension à l'auto-échauffement réduite. L'invention concerne également un procédé de fabrication d'une telle composition de biomasse. Il a été observé que la température de début d'auto-échauffement d'un AGPI-LC contenant une biomasse oléagineuse peut devenir significativement plus élevée par rupture de la biomasse et mélange de l'huile AGPI/des débris cellulaires obtenus en association avec au moins 40 % en poids d'huile végétale.
PCT/EP2020/086455 2019-12-20 2020-12-16 Procédé de réduction de la propension à l'auto-échauffement d'un agpi-lc microbien comportant une biomasse WO2021122770A1 (fr)

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US201962952175P 2019-12-20 2019-12-20
US62/952,175 2019-12-20

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WO2011054800A1 (fr) 2009-11-03 2011-05-12 Dsm Ip Assets B.V. Composition contenant des cellules et un acide gras polyinsaturé comportant au moins 20 atomes de carbone (lc-pufa)
WO2018005856A1 (fr) 2016-07-01 2018-01-04 Terravia Holdings, Inc. Ingrédients d'aliments comprenant des cellules microbiennes lysées
WO2018109059A1 (fr) * 2016-12-15 2018-06-21 Dsm Ip Assets B.V. Formulation de mélange comprenant du silicate et des cellules microbiennes et/ou végétales comprenant un acide gras polyinsaturé ayant au moins 20 atomes de carbone (agpi-lc)
WO2019185888A1 (fr) * 2018-03-29 2019-10-03 Dsm Ip Assets B.V. Nouvelle utilisation de tocophérols

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