WO2021120238A1 - 微生物自动检验设备 - Google Patents

微生物自动检验设备 Download PDF

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Publication number
WO2021120238A1
WO2021120238A1 PCT/CN2019/127784 CN2019127784W WO2021120238A1 WO 2021120238 A1 WO2021120238 A1 WO 2021120238A1 CN 2019127784 W CN2019127784 W CN 2019127784W WO 2021120238 A1 WO2021120238 A1 WO 2021120238A1
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Prior art keywords
dish
culture
temperature
area
inspection equipment
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PCT/CN2019/127784
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English (en)
French (fr)
Inventor
李晓
Original Assignee
李晓
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Publication of WO2021120238A1 publication Critical patent/WO2021120238A1/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/02—Filters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control

Definitions

  • the present invention relates to the technical field of microbial cultivation, in particular to an automatic microbial inspection equipment.
  • microorganisms In the inspection of microorganisms, the microorganisms need to be cultured. After the microorganisms are cultured for a certain period of time under suitable temperature and humidity conditions, the types and numbers of microorganisms can be observed and counted. In the process of cultivating microorganisms, it is usually necessary to cultivate multiple microorganisms simultaneously. At present, incubators with multiple temperature zones have also appeared on the market, but these incubators basically require manual operation of petri dishes in order to observe and count microorganisms.
  • microbial inspection automation equipment for the above-mentioned functions is also available on the market. Similar to the French Intel Scientific Company, in order to realize the automation of petri dish retrieval, basically all petri dishes are placed on a petri dish storage turntable, and the equipment transfers the petri dishes in time intervals according to the set program for observation and counting.
  • the petri dish storage turntable, retrieval mechanism, and imaging device are all set in the same temperature range.
  • such automated microbiological testing equipment has high accuracy for large-scale microbial culture testing at a single temperature.
  • the efficiency of the experiment can only be reduced and the experiment can be carried out in time intervals.
  • the main purpose of the present invention is to provide an automatic microbial inspection equipment to solve the technical problem that the automatic microbial inspection equipment in the prior art cannot meet the simultaneous inspection of microorganisms with multiple temperature requirements.
  • the present invention provides an automatic microbial inspection equipment.
  • the automatic microbiological inspection equipment includes: a cabin body in which an observation zone and a culture zone are formed, the observation zone and the culture zone are relatively insulated, and the culture zone is provided with many A relatively insulated culture space, the culture space is used to place the culture dishes; the imaging device is set in the observation area, and is used for image acquisition of the culture dishes; the dish-taking mechanism is set in the cabin and is used to retrieve from the culture space Petri dish; a plurality of temperature adjustment devices, respectively set in the observation area and a plurality of culture spaces, used to adjust the temperature in the observation area and the temperature in the culture space; the controller, respectively electrically connected to the plurality of temperature adjustment devices, The temperature used to control the observation area is the same as the temperature of the culture space where the culture dish to be taken out by the dish taking mechanism is located.
  • the automatic microbial inspection equipment further includes: a setter, which is electrically connected to the controller, and is used to set the temperature of each culture space.
  • the automatic microbial inspection equipment further includes: a plurality of temperature detectors, the plurality of temperature detectors are respectively arranged in the observation area and the plurality of culture spaces, and the temperature sensor arranged in the observation area is used to detect the temperature of the observation area. Temperature, a temperature sensor set in the culture space is used to detect the temperature of the culture space, and the controller is electrically connected to a plurality of temperature detectors, and is used to control the temperature of the observation area and the dish-taking mechanism according to the temperature detected by the temperature detectors. The temperature of the culture space where the petri dishes are located is the same.
  • the temperature adjustment device includes a refrigeration element and a heating element, the refrigeration element is used for cooling, and the heating element is used for heating.
  • the refrigeration element is a semiconductor refrigeration element.
  • the heating element is an electric heating element.
  • the temperature adjusting device further includes an air duct assembly, the air duct assembly is communicated with the cultivation space, the refrigeration element and the heating element are arranged on the air duct assembly, and the refrigeration element/heating element is used for the air duct assembly.
  • the airflow is used for cooling/heating, and the air duct components are used to exchange heat for the gas in the culture space.
  • the temperature adjusting device further includes a humidifying element, which is arranged on the air duct assembly and used for humidifying the airflow in the air duct assembly.
  • the temperature adjustment device further includes an ultraviolet disinfection element, which is arranged on the air duct assembly, and is used to sterilize the airflow in the air duct assembly.
  • the temperature adjusting device further includes a filter element, which is arranged on the air duct assembly and used for filtering the airflow in the air duct assembly.
  • the culture space is formed with a dish-taking mouth, and the dish-taking mouth is provided with an opening/closing door.
  • the incubation area further includes a door driver, and the door driver is connected to the opening/closing door to drive the opening/closing of the opening/closing door.
  • the plurality of culture spaces includes a first culture space and a second culture space that are arranged side by side, and the first culture space is formed with a first dish-taking opening, and the first dish-taking opening is provided with an openable/closable first culture space.
  • a door is opened and closed, a second dish-taking port is formed on the second culture space, and a second opening/closing door is provided on the second dish-taking port.
  • the door driving part includes a first door driving part and a second door driving part, The first door driver is used for driving the first opening and closing door to open above the second incubation space, and the second door driving member is used for driving the second opening and closing door to open above the first incubation space.
  • the automatic microbial inspection equipment includes: a basket, which is used to hold a culture dish, the basket is placed in the culture space, and the dish picking mechanism transfers the basket from the culture space, and grabs the culture dish from the basket.
  • the dish-taking mechanism includes: a first dish-taking mechanism, the first dish-taking mechanism is arranged at the culture area, and is used to transfer the basket in the culture space to the observation area; the second dish-taking mechanism is arranged At the observation area, it is used to take the petri dish in the basket to the imaging device for image acquisition.
  • the first dish-taking mechanism includes: a first horizontal rail, the first horizontal rail extending from the culture area to the observation area; a first vertical rail, which is movably arranged on the first horizontal rail; and a second horizontal rail.
  • the guide rail, the second horizontal guide rail is movably arranged on the first vertical guide rail; the first dish taking claw is movably installed on the second horizontal guide rail, and the first dish taking claw is used for grasping and placing the basket.
  • an area to be inspected is provided in the observation area, and the first dish-taking mechanism is used to take the basket in the culture space to the area to be inspected.
  • the second dish-taking mechanism includes; a third horizontal rail, which extends from the area to be inspected to the imaging device; a second vertical rail, which is movably arranged on the third horizontal rail; Four horizontal guide rails, the second horizontal guide rail is movably arranged on the second vertical guide rail; the second dish claw is movably installed on the fourth horizontal guide rail, and the second dish claw is used for grasping and placing the culture dish.
  • a sampling area is provided in the observation area
  • the dish-taking mechanism further includes: a third dish-taking mechanism.
  • the third dish-taking mechanism is arranged at the observation area and is used to take the petri dish in the sampling area to the waiting area.
  • the inspection area is waiting to be taken away.
  • a post-inspection area is also provided in the observation area, and the second dish-taking mechanism is also used to take the petri dish after image collection to the post-inspection area.
  • a plurality of relatively adiabatic culture spaces are provided in the culture area, and a temperature adjustment device is respectively provided in the plurality of culture spaces, so that the plurality of culture spaces can be used to meet the temperature of different types of microorganisms. Demand.
  • a temperature adjustment device is also provided in the observation area.
  • the temperature of the observation area can be controlled at the same The temperature of the culture space where the culture dish to be taken out by the dish-taking mechanism is the same, to avoid abnormal production of microorganisms in the culture dish caused by abnormal temperature in the observation area.
  • the temperature in the observation area is controlled to be the same as the temperature of the culture space where the petri dish to be taken out by the dish-taking mechanism, and the petri dish is transferred to different areas of the same temperature to avoid fogging or fogging on the petri dish. Condensate water droplets to ensure the validity of the experiment.
  • Fig. 1 shows a three-dimensional schematic diagram of an embodiment of an automatic microbial inspection device according to the present invention
  • FIG. 2 shows a schematic diagram of the three-dimensional structure of the cabin door of the embodiment of the automatic microbial inspection equipment of FIG. 1 without the cabin body;
  • Fig. 3 shows a schematic top view of the structure of the automatic microbial inspection equipment of Fig. 2;
  • Figure 4 shows a schematic front view of the structure of the automatic microbial inspection equipment of Figure 2;
  • Fig. 5 shows a three-dimensional schematic diagram of the temperature adjusting device of the automatic microbial inspection equipment according to the present invention
  • Fig. 6 shows a three-dimensional structural schematic diagram of the door driving part and the door opening and closing of the automatic microbiological inspection equipment according to the present invention
  • Fig. 7 shows a schematic diagram of the left side view of the door drive and the door opening and closing of Fig. 6.
  • FIGS 1 and 2 show an embodiment of the automatic microbiological inspection equipment of the present invention.
  • the automatic microbiological inspection equipment includes a cabin 10, an imaging device 20, a dish-taking mechanism, a plurality of temperature adjustment devices 30, and a controller.
  • An observation area 11 and a culture area 12 are formed in 10, the observation area 11 and the culture area 12 are relatively insulated, and a plurality of relatively insulated culture spaces 121 are provided in the culture area 12, and the culture spaces 121 are used for placing petri dishes.
  • the imaging device 20 is arranged in the observation area 11 and is used for image acquisition of the petri dish.
  • the dish taking mechanism is arranged in the cabin 10 and is used to retrieve the culture dish from the culture space 121.
  • a plurality of temperature adjustment devices 30 are respectively disposed in the observation area 11 and the plurality of cultivation spaces 121 for adjusting the temperature in the observation area 11 and adjusting the temperature in the cultivation space 121.
  • the controller is electrically connected to a plurality of temperature adjustment devices 30, and is used to control the temperature of the observation area 11 to be the same as the temperature of the culture space 121 where the culture dish to be taken out by the dish taking mechanism.
  • a plurality of relatively insulated cultivation spaces 121 are provided in the cultivation area 12, and the plurality of cultivation spaces 121 are respectively provided with temperature adjustment devices 30, so that the plurality of cultivation spaces 121 can be used to satisfy different types of The need for temperature for the cultivation of microorganisms.
  • the observation area 11 is also provided with a temperature adjustment device 30, and then when the imaging device 20 is about to collect the image of the petri dish in a certain culture space 121, the observation area 11
  • the temperature is controlled to be the same as the temperature of the culture space 121 where the culture dish to be taken out by the dish-taking mechanism, so as to avoid abnormal production of microorganisms in the culture dish caused by abnormal temperature in the observation area 11.
  • the temperature in the culture space 121 is different from the temperature in the observation area 11, once the petri dish at a low temperature gets the observation area 11 at a higher temperature, the petri dish
  • the image information collected by the imaging device 20 can easily calculate the water droplets or water mist as colonies in the subsequent image calculation process, which may cause errors and reduce the effectiveness of the experiment.
  • the temperature of the observation area 11 is controlled to be the same as the temperature of the culture space 121 where the petri dish to be taken out by the dish-taking mechanism is located, and the petri dish is transferred in different areas of the same temperature to avoid the raising of the petri dish. Fog or condensed water droplets ensure the validity of the experiment.
  • the cabin body 10 includes a bottom cabin and a cabin door that can be opened on the bottom cabin, an imaging device 20, a dish-taking mechanism, a plurality of temperature adjustment devices 30 and a control
  • the containers are all set on the bottom compartment, and the culture dish can be placed in the culture area 12 after the compartment door is opened.
  • the automatic microorganism inspection equipment further includes a setter, which is electrically connected with the controller, and is used to set the temperature of each culture space 121.
  • the automatic microbial inspection equipment further includes a plurality of temperature detectors, the plurality of temperature detectors are respectively arranged in the observation area 11 and the plurality of incubation spaces 121, and the temperature sensor arranged in the observation area 11 is used to detect the observation area.
  • the temperature of 11, a temperature sensor set in the culture space 121 is used to detect the temperature of the culture space 121.
  • the controller is electrically connected to a plurality of temperature detectors, and the temperature of the observation area 11 is controlled according to the temperature detected by the temperature detector to be the same as the temperature of the culture space 121 where the culture dish to be taken out by the dish taking mechanism.
  • the temperature adjusting device 30 includes a refrigerating element 31 and a heating element 32, the refrigerating element 31 is used for cooling, and the heating element 32 is used for heating.
  • the cooling element 31 can cool the culture space 121 to reduce the temperature of the culture space 121
  • the heating element 32 can heat the culture space 121 to increase the temperature of the culture space 121.
  • the temperature of the cultivation space 121 is maintained at a temperature that meets the requirements for the cultivation of microorganisms.
  • the refrigeration element 31 is a semiconductor refrigeration element, and specifically, a Peltier element can be selected to cooperate with a fan.
  • the heating element 32 is an electric heating element, and the electric heating element can be an electric heating tube or a heat radiating element for heating.
  • the temperature adjustment device 30 further includes an air duct assembly 33.
  • the air duct assembly 33 is in communication with the cultivation space 121, and the refrigeration element 31 and the heating element 32 are arranged in the air duct assembly. 33, the refrigeration element 31/heating element 32 is used to cool/heat the air flow in the air duct assembly 33, and the air duct assembly 33 is used to exchange heat for the air in the culture space 121.
  • the air duct assembly 33 includes an airflow processing air duct 331, an air inlet pipe 332 and an air outlet pipe 333 respectively connected to the airflow processing air duct 331, and an air inlet rectifier disposed on the air inlet pipe 332 and the air outlet pipe 333, respectively.
  • an air inlet fan 336 is provided between the air inlet pipe 332 and the air flow processing air duct 331, and an air outlet fan 337 is provided between the air outlet pipe 333 and the air flow processing air duct 331.
  • the temperature adjusting device 30 further includes a humidifying element 34, which is arranged on the air duct assembly 33, and is used to humidify the airflow in the air duct assembly 33, so that the cultivation space 121 can be humidified.
  • the humidity is maintained at a humidity that meets the requirements of microbial cultivation.
  • the temperature adjustment device 30 further includes an ultraviolet disinfection element 35 which is arranged on the air duct assembly 33 and is used to disinfect the airflow in the air duct assembly 33.
  • the ultraviolet disinfection element 35 can promptly dispose of the impurity microorganisms existing in the culture space 121, and prevent impurity microorganisms from affecting the accuracy of the experiment.
  • the temperature adjustment device 30 further includes a filter element 36 disposed on the air duct assembly 33 for filtering the air flow in the air duct assembly 33 to remove small particulate matter that may affect the accuracy of the experiment.
  • the culture space 121 is formed with a dish-taking mouth 1211, and the dish-taking mouth 1211 is provided with a switch door 1212 that can be opened/closed.
  • the opening and closing door 1212 can be opened from the opening 1211 of the dish, and then it can be closed again.
  • the cultivation area 12 further includes a door driving member 1213, and the door driving member 1213 is drivingly connected with the opening/closing door, and is used for driving the opening/closing of the opening/closing door.
  • the plurality of culture spaces 121 includes a first culture space and a second culture space that are arranged side by side.
  • a first dish-taking port is formed on the first culture space, and a first opening/closing door 1212a is provided on the first dish-taking port.
  • a second dish-taking port is formed on the second culture space, and a second opening/closing door 1212b is provided on the second dish-taking port.
  • the door driving member 1213 includes a first door driving member 1213a and a second door driving member 1213b, The first door driver 1213a is used for driving the first opening and closing door 1212a to open upwards of the second cultivation space, and the second door driving member 1213b is used for driving the second opening and closing doors 1212b to open upwards of the first cultivation space.
  • the way of driving the opening and closing door 1212 is the most space-saving way, which can reduce the space occupation caused by opening/closing the opening and closing door 1212.
  • the first open and close door 1212a and the second open and close door 1212b are relatively spaced in the vertical direction, and the first open and close door 1212a and the second open and close door 1212a are relatively spaced apart in the vertical direction.
  • At least one of the 1212b has a sealing baffle matched with the other one, and the vertical gap between the first open/close door 1212a and the second open/close door 1212b can be sealed by the sealing baffle.
  • the first opening and closing door 1212a and the second opening and closing door 1212b are driven to move in the horizontal direction by the first door driving member 1213a and the second door driving member 1213b, respectively.
  • the door driver includes a motor 12131, a timing belt 12132, a timing wheel 12133, a mounting plate 12134 and a sliding rail 12135.
  • the opening and closing door is fixedly connected to the timing belt 12132 through the mounting plate 12134, and is slidably installed on the sliding rail.
  • the rotation of the motor 12131 drives the rotation of the timing belt 12132 and the timing wheel 12133, and at the same time drives the opening and closing door to translate in the horizontal direction.
  • the automatic microbial inspection equipment includes a basket 40.
  • the basket 40 is used to hold a culture dish.
  • the basket 40 is placed in the culture space 121 to take the dish.
  • the mechanism transfers the basket 40 from the culture space 121 and grabs the culture dish from the basket 40.
  • the petri dishes can be placed in the basket 40 first, and then the basket 40 can be placed in the culture space 121 to facilitate storage and retrieval of a certain number of petri dishes at the same time.
  • the dish-taking mechanism includes a first dish-taking mechanism 50 and a second dish-taking mechanism 60.
  • the first dish-taking mechanism 50 is arranged at the culture area 12 and is used to transfer the basket 40 in the culture space 121 to the observation area 11, and the second dish-taking mechanism 60 is arranged at the observation area 11 and is used to place the basket 40 in the observation area 11.
  • the petri dish is taken to the imaging device 20 for image collection.
  • the first dish-taking mechanism 50 includes a first horizontal guide rail 51, a first vertical guide rail 52, a second horizontal guide rail 53, and a first dish-catching claw 54.
  • the first horizontal guide rail 51 extends from the culture area 12 to the observation area 11.
  • the first vertical rail 52 is movably installed on the first horizontal rail 51
  • the second horizontal rail 53 is movably installed on the first vertical rail 52
  • the first dish claw 54 is movably installed on the second On the horizontal rail 53
  • the first claw taking claw 54 is used for grasping and placing the basket 40.
  • the first claw taking claw 54 is movable on the second horizontal guide rail 53
  • the second horizontal guide rail 53 is movable on the first vertical guide rail 52
  • the first vertical guide rail 52 is on the first horizontal guide rail 51. It is movable, so that the first claw-taking claw 54 can move arbitrarily in the space.
  • the first horizontal guide rail 51 and the first vertical guide rail 52 are both two, which are respectively arranged in parallel, so that the first dish-taking mechanism 50 is similar to a gantry crane structure, so that the grasping and placing basket 40 is more stable.
  • the observation area 11 is provided with an area to be inspected 11a, and the first dish-taking mechanism 50 is used to carry the basket 40 in the culture space 121 After being taken to the area to be inspected 11a, the second dish taking mechanism 60 can take the petri dish in the basket 40 to the imaging device 20 for image collection.
  • the second dish taking mechanism 60 includes a third horizontal guide rail 61, a second vertical guide 62, a fourth horizontal guide 63, and a second dish taking claw 64.
  • the third horizontal guide rail 61 extends from the inspection area 11a to the imaging area.
  • the second vertical rail 62 is movably installed on the third horizontal rail 61
  • the second horizontal rail 53 is movably installed on the second vertical rail 62
  • the second dish claw 64 is movably installed On the fourth horizontal guide rail 63, the second dish claw 64 is used to hold and place the culture dish.
  • the second dish-catching claw 64 when in use, is movable on the fourth horizontal guide rail 63, the fourth horizontal guide rail 63 is movable on the second vertical guide rail 62, and the second vertical guide rail 62 is movable on the third horizontal guide rail 61, so that the second claw taking claw 64 can move arbitrarily in the space.
  • the observation area 11 is provided with a sampling area 11b
  • the dish-taking mechanism further includes a third dish-taking mechanism 70
  • the third dish-taking mechanism 70 is arranged at the observation area 11, Then take the petri dish in the sample injection area 11b to the to-be-tested area 11a and wait for it to be taken away.
  • a post-inspection area 11c is also provided in the observation area 11, and the second dish-taking mechanism 60 is also used to take the petri dish after image collection to the post-inspection area 11c.
  • the structure of the third dish taking mechanism 70 may be similar to that of the first dish taking mechanism 50 or the second dish taking mechanism 60, so as to facilitate the retrieval and retrieval of the basket 40.
  • a certain number of petri dishes may be stored by the staff, so as to wait for the third dish taking mechanism 70 to inject the petri dishes.
  • the basket 40 in the post-inspection area 11c can be removed by the staff after a certain number of petri dishes have been accumulated.
  • spatially relative terms can be used here, such as “above”, “above”, “above the surface”, “above”, etc., to describe as shown in the figure Shows the spatial positional relationship between one device or feature and other devices or features. It should be understood that the spatially relative terms are intended to encompass different orientations in use or operation in addition to the orientation of the device described in the figure. For example, if the device in the drawing is turned upside down, then a device described as “above other devices or structures” or “above other devices or structures” will then be positioned as “below the other devices or structures” or “on Under other devices or structures”. Thus, the exemplary term “above” may include both orientations “above” and “below”. The device can also be positioned in other different ways (rotated by 90 degrees or in other orientations), and the relative description of the space used here will be explained accordingly.
  • orientation words such as “front, back, up, down, left, right", “horizontal, vertical, vertical, horizontal” and “top, bottom”, etc. indicate the orientation Or positional relationship is usually based on the position or positional relationship shown in the drawings, which is only for the convenience of describing the present invention and simplifying the description. Unless otherwise stated, these positional words do not indicate or imply the pointed device or element It must have a specific orientation or be constructed and operated in a specific orientation, so it cannot be understood as a limitation of the protection scope of the present invention; the orientation word “inside and outside” refers to the inside and outside relative to the contour of each component itself.

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Abstract

一种微生物自动检验设备。该微生物自动检验设备包括舱体、成像装置、取皿机构、多个温度调节装置和控制器，其中舱体内形成有观察区和培养区，观察区和培养区相对绝热，培养区中设置有多个相对绝热的培养空间，培养空间用于放置培养皿。成像装置设置在观察区中，取皿机构设置在舱体内。多个温度调节装置分别设置在观察区和多个培养空间中。控制器与多个温度调节装置分别电连接，控制观察区的温度与取皿机构将要取出的培养皿所在的培养空间的温度相同。

Description

微生物自动检验设备 技术领域
本发明涉及微生物培养技术领域，具体而言，涉及一种微生物自动检验设备。
背景技术
在微生物的检验中需要对微生物进行培养，微生物在合适的温度和湿度条件下培养一定培养时间后，才能观察和统计微生物的类型和数量。在微生物的培养过程中，通常需要对多种微生物进行同时培养。目前，市面上也出现了具有多个温区的培养箱，但该培养箱基本都需要人工手动操作培养皿，才能实现观察和统计微生物。
此外，为了实现微生物培养、观察的自动化，市场上也出了针对上述功能的微生物检验自动化设备。类似于法国的英特科学公司，为了实现培养皿调取的自动化，基本所有的培养皿都是放置在一个培养皿储存转盘上，设备依据设定程序分时段调取培养皿进行观察和计数。
但是，上述的微生物检验自动化设备，为了实现恒温控制下的精确检测，培养皿储存转盘、调取机构以及成像装置，都是设置在同一个温度区域内。虽然，这样的微生物检验自动化设备针对单一温度下的微生物大量培养检测具有较高的准确性。但面对多温度需求的微生物培养检测时，除非增设更多数量的微生物检验自动化设备同时进行检验，不然只能降低实验效率分时段来进行实验了。
发明内容
本发明的主要目的在于提供一种微生物自动检验设备，以解决现有技术中的微生物检验自动化设备无法满足多温度需求的微生物同时进行检验的技术问题。
为了实现上述目的，本发明提供了一种微生物自动检验设备，该微生物自动检验设备包括：舱体，舱体内形成有观察区和培养区，观察区和培养区相对绝热，培养区中设置有多个相对绝热的培养空间，培养空间用于放置培养皿；成像装置，设置在观察区中，用于对培养皿进行图像采集；取皿机构，设置在舱体内，用于从培养空间中调取培养皿；多个温度调节装置，分别设置在观察区和多个培养空间中，用于调节观察区中的温度以及调节培养空间中的温度；控制器，与多个温度调节装置分别电连接，用于控制观察区的温度与取皿机构将要取出的培养皿所在的培养空间的温度相同。
在一个实施方式中，微生物自动检验设备还包括：设定器，与控制器电连接，用于设定各个培养空间的温度。
在一个实施方式中，微生物自动检验设备还包括：多个温度检测器，多个温度检测器分别设置在观察区和多个培养空间中，设置在观察区中的温度传感器用于检测观察区的温度， 设置在培养空间中的温度传感器用于检测培养空间的温度，控制器分别与多个温度检测器电连接，用于根据温度检测器检测的温度控制观察区的温度与取皿机构将要取出的培养皿所在的培养空间的温度相同。
在一个实施方式中，温度调节装置包括：制冷元件和制热元件，制冷元件用于制冷，制热元件用于制热。
在一个实施方式中，制冷元件为半导体制冷件。
在一个实施方式中，制热元件为电加热元件。
在一个实施方式中，温度调节装置还包括风道组件，风道组件与培养空间相连通，制冷元件和制热元件设置在风道组件上，制冷元件/制热元件用于对风道组件内的气流进行制冷/制热，风道组件用于对培养空间内的气体进行换热。
在一个实施方式中，温度调节装置还包括加湿元件，加湿元件设置在风道组件上，用于对风道组件内的气流进行加湿。
在一个实施方式中，温度调节装置还包括紫外消毒元件，紫外消毒元件设置在风道组件上，用于对风道组件内的气流进行消毒。
在一个实施方式中，温度调节装置还包括过滤元件，过滤元件设置在风道组件上，用于对风道组件内的气流进行过滤。
在一个实施方式中，培养空间上形成有取皿口，取皿口上设置有可打开/关闭的开关门。
在一个实施方式中，培养区还包括门驱动件，门驱动件与开关门驱动连接，用于驱动开关门打开/关闭。
在一个实施方式中，多个培养空间包括并排设置的第一培养空间和第二培养空间，第一培养空间上形成有第一取皿口，第一取皿口上设置有可打开/关闭的第一开关门，第二培养空间上形成有第二取皿口，第二取皿口上设置有可打开/关闭的第二开关门，门驱动件包括第一门驱动件和第二门驱动件，第一门驱动件用于驱动第一开关门朝向第二培养空间的上方打开，第二门驱动件用于驱动第二开关门朝向第一培养空间的上方打开。
在一个实施方式中，微生物自动检验设备包括：提篮，提篮用于盛放培养皿，提篮放置在培养空间内，取皿机构从培养空间中调取提篮，并从提篮上抓取培养皿。
在一个实施方式中，取皿机构包括：第一取皿机构，第一取皿机构设置在培养区处，用于将培养空间内的提篮调取至观察区处；第二取皿机构，设置在观察区处，用于将提篮中的培养皿取至成像装置处进行图像采集。
在一个实施方式中，第一取皿机构包括：第一水平导轨，第一水平导轨从培养区延伸至观察区；第一竖直导轨，可移动地设置在第一水平导轨上；第二水平导轨，第二水平导轨可 移动地设置在第一竖直导轨上；第一取皿爪，可移动地安装在第二水平导轨上，第一取皿爪用于抓放提篮。
在一个实施方式中，观察区中设置有待检区域，第一取皿机构用于将培养空间内的提篮取至待检区域。
在一个实施方式中，第二取皿机构包括；第三水平导轨，第三水平导轨从待检区域延伸至成像装置处；第二竖直导轨，可移动地设置在第三水平导轨上；第四水平导轨，第二水平导轨可移动地设置在第二竖直导轨上；第二取皿爪，可移动地安装在第四水平导轨上，第二取皿爪用于抓放培养皿。
在一个实施方式中，观察区中设置有进样区域，取皿机构还包括：第三取皿机构，第三取皿机构设置在观察区处，用于将进样区域的培养皿取至待检区域等待取走。
在一个实施方式中，观察区中还设置有检后区域，第二取皿机构还用于将图像采集后的培养皿取至检后区域。
应用本发明的技术方案，在培养区中设置有多个相对绝热的培养空间，并且多个培养空间中分别设置有温度调节装置，使得多个培养空间可以用于满足不同种类的微生物培养对温度的需求。同时，为了依然实现恒温控制下的精确检测，在观察区也设置有温度调节装置，再即将对某一个培养空间内的培养皿进行成像装置的图像采集时，可以将观察区的温度控制在与取皿机构将要取出的培养皿所在的培养空间的温度相同，避免在观察区由于温度异常所造成的培养皿中的微生物生产异常的情况。
此外，还有一个至关重要的原因是，当培养空间中的温度与观察区中的温度不同时，处于低温下的培养皿一旦拿到了处于较高温度的观察区后，培养皿上就会起雾、甚至凝结水滴，成像装置图像采集到的图像信息在后续的图像计算过程中就很容易将水滴或者水雾计算为菌落，进而容易出现误差，降低实验有效性。在本发明的技术方案中，将观察区的温度控制在与取皿机构将要取出的培养皿所在的培养空间的温度相同，培养皿在相同温度的不同区域转移就可以避免培养皿上起雾或者凝结水滴，保证实验有效性。
除了上面所描述的目的、特征和优点之外，本发明还有其它的目的、特征和优点。下面将参照图，对本发明作进一步详细的说明。
附图说明
构成本发明的一部分的说明书附图用来提供对本发明的进一步理解，本发明的示意性实施例及其说明用于解释本发明，并不构成对本发明的不当限定。在附图中：
图1示出了根据本发明的微生物自动检验设备的实施例的立体结构示意图；
图2示出了图1的微生物自动检验设备的实施例省去舱体的舱门的立体结构示意图；
图3示出了图2的微生物自动检验设备的俯视结构示意图；
图4示出了图2的微生物自动检验设备的主视结构示意图；
图5示出了根据本发明的微生物自动检验设备的温度调节装置的立体结构示意图；
图6示出了根据本发明的微生物自动检验设备的门驱动件及开关门的立体结构示意图；
图7示出了图6的门驱动件及开关门的左视结构示意图。
具体实施方式
需要说明的是，在不冲突的情况下，本发明中的实施例及实施例中的特征可以相互组合。下面将参考附图并结合实施例来详细说明本发明。
为了使本技术领域的人员更好地理解本发明方案，下面将结合本发明实施例中的附图，对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分的实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都应当属于本发明保护的范围。
需要说明的是，本发明的说明书和权利要求书及上述附图中的术语“第一”、“第二”等是用于区别类似的对象，而不必用于描述特定的顺序或先后次序。应该理解这样使用的术语在适当情况下可以互换，以便这里描述的本发明的实施例。此外，术语“包括”和“具有”以及他们的任何变形，意图在于覆盖不排他的包含，例如，包含了一系列步骤或单元的过程、方法、系统、产品或设备不必限于清楚地列出的那些步骤或单元，而是可包括没有清楚地列出的或对于这些过程、方法、产品或设备固有的其它步骤或单元。
需要注意的是，这里所使用的术语仅是为了描述具体实施方式，而非意图限制根据本申请的示例性实施方式。如在这里所使用的，除非上下文另外明确指出，否则单数形式也意图包括复数形式，此外，还应当理解的是，当在本说明书中使用术语“包含”和/或“包括”时，其指明存在特征、步骤、操作、器件、组件和/或它们的组合。
图1和图2示出了本发明的微生物自动检验设备的实施例，该微生物自动检验设备包括舱体10、成像装置20、取皿机构、多个温度调节装置30和控制器，其中舱体10内形成有观察区11和培养区12，观察区11和培养区12相对绝热，培养区12中设置有多个相对绝热的培养空间121，培养空间121用于放置培养皿。成像装置20设置在观察区11中，用于对培养皿进行图像采集。取皿机构设置在舱体10内，用于从培养空间121中调取培养皿。多个温度调节装置30分别设置在观察区11和多个培养空间121中，用于调节观察区11中的温度以及调节培养空间121中的温度。控制器与多个温度调节装置30分别电连接，用于控制观察区11的温度与取皿机构将要取出的培养皿所在的培养空间121的温度相同。
应用本发明的技术方案，在培养区12中设置有多个相对绝热的培养空间121，并且多个培养空间121中分别设置有温度调节装置30，使得多个培养空间121可以用于满足不同种类的微生物培养对温度的需求。同时，为了依然实现恒温控制下的精确检测，在观察区11也设置有温度调节装置30，再即将对某一个培养空间121内的培养皿进行成像装置20的图像采集 时，可以将观察区11的温度控制在与取皿机构将要取出的培养皿所在的培养空间121的温度相同，避免在观察区11由于温度异常所造成的培养皿中的微生物生产异常的情况。
此外，还有一个至关重要的原因是，当培养空间121中的温度与观察区11中的温度不同时，处于低温下的培养皿一旦拿到了处于较高温度的观察区11后，培养皿上就会起雾、甚至凝结水滴，成像装置20图像采集到的图像信息在后续的图像计算过程中就很容易将水滴或者水雾计算为菌落，进而容易出现误差，降低实验有效性。在本发明的技术方案中，将观察区11的温度控制在与取皿机构将要取出的培养皿所在的培养空间121的温度相同，培养皿在相同温度的不同区域转移就可以避免培养皿上起雾或者凝结水滴，保证实验有效性。
如图1所示，在本实施例的技术方案中，舱体10包括底舱和可打开地设置在底舱上的舱门，成像装置20、取皿机构、多个温度调节装置30和控制器都设置在底舱上，舱门打开后可以往培养区12放置培养皿。
可选的，在本实施例的技术方案中，微生物自动检验设备还包括设定器，设定器与控制器电连接，用于设定各个培养空间121的温度。通过设定器，用户可以根据实际的培养需求，针对不同种类的微生物培养设定不同的温度。更为优选的，微生物自动检验设备还包括多个温度检测器，多个温度检测器分别设置在观察区11和多个培养空间121中，设置在观察区11中的温度传感器用于检测观察区11的温度，设置在培养空间121中的温度传感器用于检测培养空间121的温度。在实际使用时，控制器分别与多个温度检测器电连接，根据温度检测器检测的温度控制观察区11的温度与取皿机构将要取出的培养皿所在的培养空间121的温度相同。
如图5所示，在本实施例的技术方案中，温度调节装置30包括制冷元件31和制热元件32，制冷元件31用于制冷，制热元件32用于制热。在使用时，通过制冷元件31可以对培养空间121制冷以降低培养空间121的温度，通过制热元件32可以对培养空间121制热以提高培养空间121的温度，通过上述两个部件就可以将培养空间121的温度维持在符合微生物培养需求的温度。作为一种可选的，制冷元件31为半导体制冷件，具体的可以选择帕尔贴元件配合风扇。可选的，制热元件32为电加热元件，电加热元件可以选择电加热管或者热辐射元件进行加热。
如图5所示，在本实施例的技术方案中，温度调节装置30还包括风道组件33，风道组件33与培养空间121相连通，制冷元件31和制热元件32设置在风道组件33上，制冷元件31/制热元件32用于对风道组件33内的气流进行制冷/制热，风道组件33用于对培养空间121内的气体进行换热。具体的，风道组件33包括气流处理风道331、分别与气流处理风道331相连的进风管332和出风管333、分别设置在进风管332和出风管333上的进风整流罩335和出风整流罩334。优选的，在进风管332和气流处理风道331之间设置有进风风机336，在出风管333和气流处理风道331之间设置有出风风机337。
作为一种更为优选的实施方式，温度调节装置30还包括加湿元件34，加湿元件34设置在风道组件33上，用于对风道组件33内的气流进行加湿，进而可以将培养空间121的湿度 维持在符合微生物培养需求的湿度。更为优选的，温度调节装置30还包括紫外消毒元件35，紫外消毒元件35设置在风道组件33上，用于对风道组件33内的气流进行消毒。通过紫外消毒元件35可以及时处理掉存在于培养空间121中的杂质微生物，避免杂质微生物影响实验的精确性。更为优选的，温度调节装置30还包括过滤元件36，过滤元件设置在风道组件33上，用于对风道组件33内的气流进行过滤，以去除可能影响实验精确性的小颗粒物质。
如图2和图3所示，在本实施例的技术方案中，培养空间121上形成有取皿口1211，取皿口1211上设置有可打开/关闭的开关门1212，在放置或者调取培养皿时，就可以将开关门1212从取皿口1211上打开，之后再关闭即可。更为优选的，培养区12还包括门驱动件1213，门驱动件1213与开关门驱动连接，用于驱动开关门打开/关闭。多个培养空间121包括并排设置的第一培养空间和第二培养空间，第一培养空间上形成有第一取皿口，第一取皿口上设置有可打开/关闭的第一开关门1212a，第二培养空间上形成有第二取皿口，第二取皿口上设置有可打开/关闭的第二开关门1212b，门驱动件1213包括第一门驱动件1213a和第二门驱动件1213b，第一门驱动件1213a用于驱动第一开关门1212a朝向第二培养空间的上方打开，第二门驱动件1213b用于驱动第二开关门1212b朝向第一培养空间的上方打开。该驱动打开开关门1212的方式为最节省空间的方式，可以减少为了打开/关闭的开关门1212而造成的空间占用。
具体的，在本实施例的技术方案中，如图6和图7所示，第一开关门1212a和第二开关门1212b在竖直方向上相对间隔，第一开关门1212a和第二开关门1212b中的至少一个具有与另一个相配合的密封挡板，通过密封挡板可以密封第一开关门1212a和第二开关门1212b在竖直方向上的间隙。在使用时，第一开关门1212a和第二开关门1212b分别通过第一门驱动件1213a和第二门驱动件1213b驱动在水平方向上移动。如图7所示，门驱动件包括电机12131、同步带12132、同步轮12133、安装板12134和滑轨12135，开关门通过安装板12134与同步带12132固定连接，并且可滑动地安装在滑轨12135上，电机12131转动带动同步带12132和同步轮12133转动，同时带动开关门在水平方向上平移。
更为优选的，如图2和图4所示，在本发明的技术方案中，微生物自动检验设备包括提篮40，提篮40用于盛放培养皿，提篮40放置在培养空间121内，取皿机构从培养空间121中调取提篮40，并从提篮40上抓取培养皿。在实际放置培养皿时，可以先将培养皿放置到提篮40中，再将提篮40放置到培养空间121中，以方便同时存放和调取一定数量的培养皿。
如图2、图3和图4所示，在本实施例的技术方案中，取皿机构包括第一取皿机构50和第二取皿机构60。第一取皿机构50设置在培养区12处，用于将培养空间121内的提篮40调取至观察区11处，第二取皿机构60设置在观察区11处，用于将提篮40中的培养皿取至成像装置20处进行图像采集。具体的，第一取皿机构50包括第一水平导轨51、第一竖直导轨52、第二水平导轨53以及第一取皿爪54，第一水平导轨51从培养区12延伸至观察区11，第一竖直导轨52可移动地设置在第一水平导轨51上，第二水平导轨53可移动地设置在第一竖直导轨52上，第一取皿爪54可移动地安装在第二水平导轨53上，第一取皿爪54用于抓放提篮40。在使用时，第一取皿爪54在第二水平导轨53上可移动，第二水平导轨53在第一竖直导 轨52上可移动，第一竖直导轨52又在第一水平导轨51上可移动，从而可以实现第一取皿爪54在空间内任意移动。可选的，第一水平导轨51和第一竖直导轨52均为两根，分别平行设置，使得第一取皿机构50类似于龙门吊结构，使得抓放提篮40更加稳定。
更为优选的呢，如图2和图3所示，在本实施例的技术方案中，观察区11中设置有待检区域11a，第一取皿机构50用于将培养空间121内的提篮40取至待检区域11a，之后第二取皿机构60就可以将提篮40中的培养皿取至成像装置20出进行图像采集。
可选的，第二取皿机构60包括第三水平导轨61、第二竖直导轨62、第四水平导轨63以及第二取皿爪64，第三水平导轨61从待检区域11a延伸至成像装置20处，第二竖直导轨62可移动地设置在第三水平导轨61上，第二水平导轨53可移动地设置在第二竖直导轨62上，第二取皿爪64可移动地安装在第四水平导轨63上，第二取皿爪64用于抓放培养皿。与第一取皿机构50相似，在使用时，第二取皿爪64在第四水平导轨63上可移动，第四水平导轨63在第二竖直导轨62上可移动，第二竖直导轨62又在第三水平导轨61上可移动，从而可以实现第二取皿爪64在空间内任意移动。
可选的，在本实施例的技术方案中，观察区11中设置有进样区域11b，取皿机构还包括第三取皿机构70，第三取皿机构70设置在观察区11处，用于将进样区域11b的培养皿取至待检区域11a等待取走。更为优选的，在本实施例的技术方案中，观察区11中还设置有检后区域11c，第二取皿机构60还用于将图像采集后的培养皿取至检后区域11c。可选的，第三取皿机构70的结构可以和第一取皿机构50或第二取皿机构60相似，以实现方便调取提篮40为宜。可选的，进样区域11b中的提篮40，可以由工作人员存放一定数量培养皿，以等待第三取皿机构70进行培养皿的进样。检后区域11c中的提篮40，可以在积攒一定数量的培养皿后，再由工作人员取走提篮40。
除非另外具体说明，否则在这些实施例中阐述的部件和步骤的相对布置、数字表达式和数值不限制本发明的范围。同时，应当明白，为了便于描述，附图中所示出的各个部分的尺寸并不是按照实际的比例关系绘制的。对于相关领域普通技术人员已知的技术、方法和设备可能不作详细讨论，但在适当情况下，所述技术、方法和设备应当被视为授权说明书的一部分。在这里示出和讨论的所有示例中，任何具体值应被解释为仅仅是示例性的，而不是作为限制。因此，示例性实施例的其它示例可以具有不同的值。应注意到：相似的标号和字母在下面的附图中表示类似项，因此，一旦某一项在一个附图中被定义，则在随后的附图中不需要对其进行进一步讨论。
为了便于描述，在这里可以使用空间相对术语，如“在……之上”、“在……上方”、“在……上表面”、“上面的”等，用来描述如在图中所示的一个器件或特征与其他器件或特征的空间位置关系。应当理解的是，空间相对术语旨在包含除了器件在图中所描述的方位之外的在使用或操作中的不同方位。例如，如果附图中的器件被倒置，则描述为“在其他器件或构造上方”或“在其他器件或构造之上”的器件之后将被定位为“在其他器件或构造下方”或“在其他器件或构造之下”。因而，示例性术语“在……上方”可以包括“在……上方”和“在…… 下方”两种方位。该器件也可以其他不同方式定位(旋转90度或处于其他方位)，并且对这里所使用的空间相对描述作出相应解释。
在本发明的描述中，需要理解的是，方位词如“前、后、上、下、左、右”、“横向、竖向、垂直、水平”和“顶、底”等所指示的方位或位置关系通常是基于附图所示的方位或位置关系，仅是为了便于描述本发明和简化描述，在未作相反说明的情况下，这些方位词并不指示和暗示所指的装置或元件必须具有特定的方位或者以特定的方位构造和操作，因此不能理解为对本发明保护范围的限制；方位词“内、外”是指相对于各部件本身的轮廓的内外。
以上所述仅为本发明的优选实施例而已，并不用于限制本发明，对于本领域的技术人员来说，本发明可以有各种更改和变化。凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。

Claims (20)

  1. 一种微生物自动检验设备，其特征在于，包括：
    舱体(10)，所述舱体(10)内形成有观察区(11)和培养区(12)，所述观察区(11)和所述培养区(12)相对绝热，所述培养区(12)中设置有多个相对绝热的培养空间(121)，所述培养空间(121)用于放置培养皿；
    成像装置(20)，设置在所述观察区(11)中，用于对培养皿进行图像采集；
    取皿机构，设置在所述舱体(10)内，用于从所述培养空间(121)中调取培养皿；
    多个温度调节装置(30)，分别设置在所述观察区(11)和多个所述培养空间(121)中，用于调节所述观察区(11)中的温度以及调节所述培养空间(121)中的温度；
    控制器，与所述多个温度调节装置(30)分别电连接，用于控制所述观察区(11)的温度与所述取皿机构将要取出的培养皿所在的培养空间(121)的温度相同。
  2. 根据权利要求1所述的微生物自动检验设备，其特征在于，所述微生物自动检验设备还包括：设定器，与所述控制器电连接，用于设定各个所述培养空间(121)的温度。
  3. 根据权利要求1所述的微生物自动检验设备，其特征在于，所述微生物自动检验设备还包括：多个温度检测器，所述多个温度检测器分别设置在所述观察区(11)和多个所述培养空间(121)中，设置在所述观察区(11)中的温度传感器用于检测所述观察区(11)的温度，设置在所述培养空间(121)中的温度传感器用于检测所述培养空间(121)的温度，所述控制器分别与多个温度检测器电连接，用于根据所述温度检测器检测的温度控制所述观察区(11)的温度与所述取皿机构将要取出的培养皿所在的培养空间(121)的温度相同。
  4. 根据权利要求1所述的微生物自动检验设备，其特征在于，所述温度调节装置(30)包括：制冷元件(31)和制热元件(32)，所述制冷元件(31)用于制冷，所述制热元件(32)用于制热。
  5. 根据权利要求4所述的微生物自动检验设备，其特征在于，所述制冷元件(31)为半导体制冷件。
  6. 根据权利要求4所述的微生物自动检验设备，其特征在于，所述制热元件(32)为电加热元件。
  7. 根据权利要求4所述的微生物自动检验设备，其特征在于，所述温度调节装置(30)还包括风道组件(33)，所述风道组件(33)与所述培养空间(121)相连通，所述制冷元件(31)和所述制热元件(32)设置在所述风道组件(33)上，所述制冷元件(31)/所述制热元件(32)用于对所述风道组件(33)内的气流进行制冷/制热，所述风道组件(33)用于对所述培养空间(121)内的气体进行换热。
  8. 根据权利要求7所述的微生物自动检验设备，其特征在于，所述温度调节装置(30)还包括加湿元件(34)，所述加湿元件(34)设置在所述风道组件(33)上，用于对所述风道组件(33)内的气流进行加湿。
  9. 根据权利要求7所述的微生物自动检验设备，其特征在于，所述温度调节装置(30)还包括紫外消毒元件(35)，所述紫外消毒元件(35)设置在所述风道组件(33)上，用于对所述风道组件(33)内的气流进行消毒。
  10. 根据权利要求7所述的微生物自动检验设备，其特征在于，所述温度调节装置(30)还包括过滤元件(36)，所述过滤元件设置在所述风道组件(33)上，用于对所述风道组件(33)内的气流进行过滤。
  11. 根据权利要求1所述的微生物自动检验设备，其特征在于，所述培养空间(121)上形成有取皿口(1211)，所述取皿口(1211)上设置有可打开/关闭的开关门(1212)。
  12. 根据权利要求11所述的微生物自动检验设备，其特征在于，所述培养区(12)还包括门驱动件(1213)，所述门驱动件(1213)与所述开关门(1212)驱动连接，用于驱动所述开关门(1212)打开/关闭。
  13. 根据权利要求12所述的微生物自动检验设备，其特征在于，多个所述培养空间(121)包括并排设置的第一培养空间和第二培养空间，所述第一培养空间上形成有第一取皿口，所述第一取皿口上设置有可打开/关闭的第一开关门(1212a)，所述第二培养空间上形成有第二取皿口，所述第二取皿口上设置有可打开/关闭的第二开关门(1212b)，所述门驱动件(1213)包括第一门驱动件(1213a)和第二门驱动件(1213b)，所述第一门驱动件(1213a)用于驱动第一开关门(1212a)朝向所述第二培养空间的上方打开，所述第二门驱动件(1213b)用于驱动所述第二开关门(1212b)朝向所述第一培养空间的上方打开。
  14. 根据权利要求1所述的微生物自动检验设备，其特征在于，所述微生物自动检验设备包括：提篮(40)，所述提篮(40)用于盛放培养皿，所述提篮(40)放置在所述培养空间(121)内，所述取皿机构从所述培养空间(121)中调取提篮(40)，并从所述提篮(40)上抓取所述培养皿。
  15. 根据权利要求14所述的微生物自动检验设备，其特征在于，所述取皿机构包括：
    第一取皿机构(50)，所述第一取皿机构(50)设置在所述培养区(12)处，用于将所述培养空间(121)内的提篮(40)调取至所述观察区(11)处；
    第二取皿机构(60)，设置在所述观察区(11)处，用于将所述提篮(40)中的培养皿取至成像装置(20)处进行图像采集。
  16. 根据权利要求15所述的微生物自动检验设备，其特征在于，所述第一取皿机构(50)包括：
    第一水平导轨(51)，所述第一水平导轨(51)从所述培养区(12)延伸至所述观察 区(11)；
    第一竖直导轨(52)，可移动地设置在所述第一水平导轨(51)上；
    第二水平导轨(53)，所述第二水平导轨(53)可移动地设置在所述第一竖直导轨(52)上；
    第一取皿爪(54)，可移动地安装在所述第二水平导轨(53)上，所述第一取皿爪(54)用于抓放提篮(40)。
  17. 根据权利要求16所述的微生物自动检验设备，其特征在于，所述观察区(11)中设置有待检区域(11a)，所述第一取皿机构(50)用于将所述培养空间(121)内的提篮(40)取至所述待检区域(11a)。
  18. 根据权利要求17所述的微生物自动检验设备，其特征在于，所述第二取皿机构(60)包括；
    第三水平导轨(61)，所述第三水平导轨(61)从所述待检区域(11a)延伸至所述成像装置(20)处；
    第二竖直导轨(62)，可移动地设置在所述第三水平导轨(61)上；
    第四水平导轨(63)，所述第二水平导轨(53)可移动地设置在所述第二竖直导轨(62)上；
    第二取皿爪(64)，可移动地安装在所述第四水平导轨(63)上，所述第二取皿爪(64)用于抓放培养皿。
  19. 根据权利要求18所述的微生物自动检验设备，其特征在于，所述观察区(11)中设置有进样区域(11b)，所述取皿机构还包括：
    第三取皿机构(70)，所述第三取皿机构(70)设置在所述观察区(11)处，用于将所述进样区域(11b)的培养皿取至所述待检区域(11a)等待取走。
  20. 根据权利要求19所述的微生物自动检验设备，其特征在于，所述观察区(11)中还设置有检后区域(11c)，所述第二取皿机构(60)还用于将图像采集后的培养皿取至所述检后区域(11c)。
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