WO2012141055A1 - 細胞培養装置、及び搬送装置 - Google Patents
細胞培養装置、及び搬送装置 Download PDFInfo
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- WO2012141055A1 WO2012141055A1 PCT/JP2012/059224 JP2012059224W WO2012141055A1 WO 2012141055 A1 WO2012141055 A1 WO 2012141055A1 JP 2012059224 W JP2012059224 W JP 2012059224W WO 2012141055 A1 WO2012141055 A1 WO 2012141055A1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/14—Bags
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/50—Means for positioning or orientating the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
Definitions
- the present invention relates to a cell culture apparatus for culturing cells, and particularly to an automatic culture technique for efficiently culturing cells with aseptic cleanliness.
- Patent Document 1 provides an example in which a culture container transport operation, a medium exchange, and the like are processed by an articulated robot manipulator.
- This articulated robot manipulator presents a configuration that can sterilize itself.
- Patent Document 2 provides a method of treating the culture vessel and the flow path itself in a closed system. This is a device that cultivates chondrocytes under high pressure. The culture vessel and flow channel are sealed, and the culture vessel and partly plugged flow channel are taken out so as not to touch the outside air after the culture is completed. Means for recovering aseptically generated cartilage tissue.
- Patent Document 2 since it is a closed system, a flow path is attached while maintaining the sterility of the inside, and a medium (culture solution) or a cell (cell suspension) is placed in the flow path. The problem is whether to inject it. In addition, when carrying out complicated culture work, it is a problem whether the sealed flow path can be easily installed in the apparatus and the driving force is efficiently given.
- a system in which the culture vessel itself for automatic culture has a closed system structure, the inside of which can be sterilized, and a cell is cultured by supplying driving force from the outside is preferable.
- the configuration of the flow path is essential, minimizing the manufacturing error of this closed system flow path, and making the closed system flow path efficient, safe and aseptic. It will be a problem whether to install it in
- a problem is how to simplify and increase the efficiency of the mechanism for performing culture processing and controlling the culture medium and cells aseptically.
- an object of the present invention is to provide a cell culture apparatus provided with a mechanism for preventing a mistake and efficiently installing a closed system flow path in a drive system aseptically.
- another object of the present invention is to provide a transport device for aseptically supplying a cell fluid or the like into a closed channel of the cell culture device, and to inject a liquid such as a sterile cell fluid or the like. It is to provide a method.
- a cell culture apparatus for culturing cells using a culture medium, wherein the culture medium and cells are supplied from a plurality of bags having the culture medium and cells, and a part of the plurality of bags.
- a first module having a first flow path group for discharging the culture medium and cells to the other part of the plurality of bags, a culture container for culturing the cells, supplying the culture medium and cells to the culture container, and culturing
- a second module having a second flow path group for discharging the culture medium and cells from the container, and the culture medium and cells flowing in from the flow path in the first flow path group are retained, and the retained culture medium and cells are cultured.
- the infusion tank that flows out to the flow path in the second flow path group, and the medium and cells that are discharged from the culture container and flow in from the flow path in the second flow path group are held and held. Drain the cultured media and cells to the other part of the multiple bags Therefore, a tank portion having a waste liquid tank that flows out to the flow path in the first flow path group, and the amount of the medium and cell feeding in the flow paths in the first and second flow path groups, And a third module having a pump unit that controls the amount of the culture medium and the cells held in the tank unit.
- a cell culture apparatus for culturing cells using a medium, a plurality of bags having the medium and cells, and the medium and cells from a part of the plurality of bags.
- a first module that has a first flow path group for discharging the culture medium and cells to the other part of the plurality of bags, is held by the holding base, a culture vessel for culturing the cells, and culture
- a second module that has a second flow path group for supplying the culture medium and cells to the container and discharging the culture medium and the cells from the culture container, and the flow path in the first flow path group;
- the injection tank that flows out to the flow path in the second flow path group and the culture container are discharged from the culture container.
- a tank unit having a waste liquid tank that flows out to the flow path in the first flow path group, and the first and second flow path groups
- a third module that has a pump unit that controls the amount of the medium and cells fed inside the flow path and the amount of the medium and cells held inside the tank unit, and is held by the holding base;
- a cell culture apparatus having a configuration comprising:
- the closed system flow path is composed of three module groups.
- This module group includes cell modules and media that need to be refrigerated, medium modules using washing liquid and waste liquid, pump modules that pump these liquids into the culture chamber and heat them in the tank, and cell solutions and culture media from the tank to the culture vessel
- It consists of a culture vessel module that feeds into the internal culture space and performs culture processing. There is a microscope around the culture container module, and the cells on the culture surface can be imaged.
- Each module is installed in a holder called a holding base and having a drive mechanism in part.
- the closed system flow path can be integrated and removed from the inside of the culture apparatus by the transport jig capable of detaching the holding base. All the modules are connected to the tank, and the modules are not connected directly.
- a transport device that can transport and detach a plurality of bags of a culture medium and cells.
- a first holder for holding a medium base provided with a first flow path group for supplying cells and discharging the medium and cells to another part of the plurality of bags, a culture container for culturing the cells, and a culture container
- a transport device having a third holding tool for holding a drive base provided with a pump unit for controlling the amount of medium and cell feeding in a path.
- a liquid injection method for injecting cells into a cell bag that is transported to and detached from the cell culture device, and a plurality of bags including the cell bag A culture medium base including a first flow path group for supplying culture medium and cells from a part of the plurality of bags and discharging the culture medium and cells to the other part of the plurality of bags, a culture container for culturing the cells, and culture A culture container base including a second flow path group for supplying the culture medium and cells to the container and discharging the culture medium and the cells from the culture container, and the culture medium and cells inside the flow paths in the first and second flow path groups
- a transport device equipped with a holder that holds a drive base with a pump unit that controls the amount of liquid to be fed is transported to the vicinity of the cell direct treatment area, and the cell bag connected to the flow path is directly transferred from the medium base to the cell. Move to the treatment area Directly at the processing zone, the cells were injected into
- the present invention it is easy to install a closed flow channel having a culture vessel, prevents mistakes during flow channel production and installation, and further sterilizes cells with aseptic cleanliness at an appropriate production standard level. It is possible to realize a cell culturing apparatus that can efficiently cultivate.
- the culture vessel and the flow channel itself can be a closed system structure, and a transport device for aseptically supplying cell fluid, medium, etc. to the flow channel can be provided. Furthermore, it is possible to provide a method for injecting a liquid such as a sterile cell solution or culture medium into the culture vessel and the channel.
- FIG. 1 shows the whole schematic structure of the automatic culture apparatus which installed the drive base based on a 1st Example. It is a front view which shows the automatic culture apparatus which opened the door based on a 1st Example. It is a side view which shows the inside of the automatic culture apparatus based on a 1st Example. It is a block diagram which shows the state before connecting a sealing mechanism to the nail
- the closed system flow path is mainly composed of three modules.
- the interior of the automatic culture device consists of a culture room, a refrigerator room, and an intermediate room, each with a door.
- the refrigerator compartment maintains approximately 4 ° C.
- the culture chamber interior maintains a state close to 37 ° C., 5% carbon dioxide concentration, and humidity 100%. It has a waste liquid recovery port with a check valve in the cell compartment, cell suspension, medium, washing liquid and waste liquid.
- an intermediate chamber is provided between them, and the area other than the tube for feeding liquid is sealed with a rubber plug or other seal. Can be divided.
- a fan with a filter is provided in the intermediate chamber, and the environment outside the automatic culture apparatus can be maintained. As a result, although the environment is completely different in the same apparatus, the best environment for the cells can be installed, and space saving is possible.
- the base is moved to the vicinity of the safety cabinet (or clean bench) with the transfer jig, the empty bag in the closed system flow path that has been sterilized inside is sterilized and placed inside the safety cabinet, and the cell suspension (
- the inside of the closed system flow path can be maintained in a sterile state regardless of the environment outside the closed system flow path by injecting a medium, a washing solution, or the like) into the bag and sealing the injection port.
- the drive mechanism is installed outside the closed system flow path. While keeping the inside sterile, it is possible to perform automatic cell culture operations such as cell seeding, medium exchange, microscopic observation, and collection of waste liquid for inspection.
- the present embodiment is merely an example for realizing the present invention and does not limit the technical scope of the present invention.
- the same reference numerals are assigned to common configurations.
- the terms culture vessel base and medium base are used.
- the term “holding base” and “culture container” are used as a holding base because it has a function as a holder.
- the first, second, and third holders of the transfer device that holds the base and the drive base may be collectively referred to as a holder.
- FIG. 1 is an overall schematic diagram of an automatic culture apparatus 10 according to the first embodiment.
- FIG. 2 is a front view of the automatic culture apparatus 10.
- FIG. 3 is a side view of the automatic culture apparatus 10.
- 4A and 4B are schematic diagrams relating to the seal 33 between the cell culture chamber 13 and the refrigerator 14.
- FIG. 5 is a schematic diagram regarding the configuration of the flow path 40.
- FIG. 6 is a schematic view relating to the tank 25 which is one of the components of the flow path 40.
- FIG. 7A, 7B, 7C, 7D, and 7E are schematic diagrams relating to the pump 23 that is one of the components of the flow path 40.
- FIG. 8A and 8B are schematic diagrams relating to the supply of aseptic cell solution and medium into the flow path 40.
- FIG. 9 is a block diagram showing a circuit for operating the automatic culture apparatus 10.
- cell modules and media that need to be refrigerated, medium modules such as washing liquid and waste liquid, and such liquids are pumped into the culture chamber and stored in a tank.
- a pump module for heating, and a culture container module for sending a cell solution and a medium from a tank to a culture space inside the culture container and performing a culture process are provided.
- Each module is connected to the tank, and basically the modules are not connected to each other.
- it is possible to manufacture a flow channel in units of modules and install it on each holding base.
- each module is independent, it is possible to organize the flow channel and prevent erroneous connection and space saving.
- the overall configuration of the automatic culture apparatus 10 will be described with reference to FIGS. 1, 2, 3, 4 ⁇ / b> A, and 4 ⁇ / b> B.
- the automatic culture apparatus 10 includes a cell culture chamber 13, a refrigerator 14, a control unit 15, and an intermediate chamber 16 as basic components. Each basic configuration will be described below.
- the automatic culture apparatus 10 as a whole is composed of a cell culture chamber 13, a refrigerator 14 constituting a refrigeration unit, a control unit 15, and an intermediate chamber 16. It is possible to access the inside of the automatic culture apparatus 10 by opening the door 12 and the intermediate chamber door 17.
- a holding base for holding and setting the culture container 20 a culture container base 21 that is a holder, a rotating mechanism 22 that rotates by being connected thereto, and a microscope 28 that captures a cell image in the culture container 20.
- a drive base 27 as a holding base that has a drive mechanism such as a stage 29, a pump 23, and a valve 24 and is connected to a flow path 40 having a closed system structure including a tank 25.
- the rotation mechanism 22 is fixed to one end of the drive base 27, and the culture vessel base 21 and the drive base 27 are connected via the rotation mechanism 22. Furthermore, there is a connector board 30 that is connected to all the internal drive mechanisms and wired to the control unit 15. The wiring between the control unit 15 and the connector board 30 is not shown.
- the inside of the cell culture chamber 13 maintains an environment close to a temperature of 37 ° C., humidity of 100%, and 5% carbon dioxide. However, only the peripheral part including the culture vessel 20 can be configured to maintain the aforementioned environment as necessary.
- the culture medium base 32 can be placed in the refrigerator 14, and the cell culture chamber 13 and the refrigerator compartment are provided with a seal 33 that allows only the tube 41 of the flow path 40 to pass while the three sections of the cell culture chamber 13, the refrigerator 14, and the intermediate chamber 16 are independent. It is possible to keep the temperature inside 14 and prevent condensation.
- the intermediate chamber 16 has a fan 50 with a filter 51.
- the filter 51 is installed at the exhaust port. It is possible to prevent an increase in humidity by causing movement of the airflow by the fan 50.
- a seal mechanism that shuts off the inside of the cell culture chamber 13 and the inside of the refrigerator 14 and flows only the culture solution is used at the entrance of the intermediate chamber 16 described in detail later.
- the culture medium base 32 is connected to the drive base 27 by a tube 41 that passes through the seal 33.
- the control unit 15 is independent of other compartments and is installed below the cell culture 13 and the intermediate chamber 16. As a result, the temperature, humidity, and carbon dioxide in the cell culture chamber 13 are blocked to protect the internal electrical equipment.
- the control unit 15 includes a fan 52 that allows the intake heat 53 and the exhaust filter 54 to dissipate internal heat to the outside aseptically.
- the fan 52 and the filters 53 and 54 constitute a cooling unit of the control unit 15.
- Reference numeral 57 denotes a control panel of the control unit 15, which includes various buttons, a display unit, and the like, similar to a normal operation panel, and is used for the operation of the control unit 15.
- the control unit 15 includes a central processing unit (CPU) that is a processing unit (not shown), a memory that is a storage unit that stores control programs and data, and the like.
- CPU central processing unit
- memory that is a storage unit that stores control programs and data, and the like.
- FIG. 2 shows the arrangement of each component device viewed from the front of the automatic culture apparatus 10 with the cell culture chamber door 11 and the refrigerator door 12 opened. Centering around the drive base 27 inside the cell culture chamber 13, the culture vessel 20 is configured to be rotated in the horizontal direction, and the refrigerator 14 is provided with a culture medium base 32 containing the cell solution and culture medium in the vertical direction. .
- the space (first space) in which the drive base 27 is installed is the first quadrant, the first space and the refrigeration unit.
- the intermediate chamber 16 that partitions the refrigerator 14 is positioned in the fourth quadrant, and the culture container base 21 that is a holding member on which the culture container 20 is held and placed is positioned in the second quadrant or the third quadrant by rotation.
- a space-saving configuration with a short distance between each device is obtained, and the medium stored at around 4 ° C. in the refrigerator 14 located in the fourth quadrant is placed on the drive base 27 located in the first quadrant. It can be warmed in the tank 25 and quickly supplied to the culture space located in the second quadrant, and cell damage due to movement can be minimized.
- the microscope 28 for imaging the cells is configured in the vertical direction from the culture vessel 20 located in the second quadrant as viewed from the front.
- the microscope 28 is fixed to a stage 29 for movement and focusing.
- the driving base 27 is provided with a driving mechanism such as a pump 23 for feeding the liquid in the flow path 40, a valve 24 for switching a liquid feeding circuit, and a tank 25 for warming the medium and changing the flow direction of the liquid. Details will be described later.
- a connector board 30 is provided on the back surface of the drive base 27 and can be connected to the connector 31 of the drive base 27. Wiring from the connector board 30 to the control unit 15 is possible.
- the connector board 30 has a heat insulation / waterproof structure in order to connect the wiring of the connector 31 to the external control unit 15 while maintaining the environment inside the cell culture chamber 13.
- the control unit 15 is described in the vertical direction of the cell culture chamber 13 in this figure, for example, when used on a tabletop, it may be installed in a horizontal direction or in an extra space.
- FIG. 3 shows the arrangement of each component device as viewed from the side of the automatic culture apparatus 10.
- the cell culture chamber 13 and the refrigerator 14 having completely different environments such as temperature and humidity are arranged in the same apparatus, and the intermediate chamber 16 and the seal 33 are installed in order to connect the flow path 40.
- the cell culture chamber 13 generally has a temperature of 37 ° C. and 100% humidity, and the refrigerator 14 generally holds the interior at 4 to 5 ° C. Therefore, when the cell culture chamber 13 is installed close to the cell culture chamber 13, condensation occurs. In addition, temperature unevenness inside the cell culture chamber 13 occurs.
- General incubators (cell culture rooms) and refrigerators are designed to be used at room temperature and indoor humidity.
- the culture medium, the washing liquid, and the cell suspension in the refrigerator 14 can be fed into the cell culture chamber 13 through the tube 41 that constitutes the flow path 40.
- the space of the refrigerator 14, the intermediate chamber 16, and the cell culture chamber 13 is separated by inserting the tube 41 into the groove 42 of the seal 33 as shown in FIGS.
- the suspension can be sent from the refrigerator 14 to the cell culture chamber 13.
- the claws 34 are respectively provided on a wall 13 ⁇ / b> A that partitions the cell culture device 13 and the intermediate chamber 16, and a wall 14 ⁇ / b> A that partitions the refrigerator 14 and the intermediate chamber 16.
- the refrigerator 14 has a cooler 18 for cooling the inside thereof. This discharges the cooled heat to the outside of the refrigerator 14.
- air that has passed through the filter 51 from the outside of the automatic culture apparatus 10 is sucked by the fan 50 and applied to the cooler 18, and is discharged to the outside of the automatic culture apparatus 10 through the filter 51. It was set as the structure to do. That is, the cooler 18, the fan 50, and further the filter 51 constitute a cooling unit for the intermediate chamber.
- the air flow is shown by the arrow 55 in FIG. Thereby, the air temperature and humidity inside the intermediate chamber 16 can maintain the same state as the outside of the automatic culture apparatus 10.
- air outside the control unit 15 is introduced by the fan 52 through the intake filter 53, and returned to the outside of the control unit 15 through the exhaust filter 54.
- the air flow 56 is shown by the arrows in FIG. Thereby, for example, dust can be prevented from being scattered in a clean area of a clean room, and the cleanliness can be maintained.
- FIG. 5 is a schematic diagram showing the overall configuration of the flow path 40, the tank 25, and the drive base 27.
- FIG. 6 is a front view showing the configuration of the tank 25.
- 7A shows a top view of a state where the syringe 43 and the pump 23 are separated
- FIG. 7B shows a top view of a state where the syringe 43 and the pump 23 are integrated
- FIG. 7C shows a state where the syringe 43 and the pump 23 are separated.
- FIG. 7D shows a side view of the syringe 43 set in the pump 23, and FIG. 7E shows a side view of the syringe 43 and the pump 23 integrated into the syringe pump 44.
- the syringe 43 can translate on the same axis.
- a syringe pump is used as the driving pump.
- other pumps such as a tube pump can be used as the driving pump.
- the flow path 40 is exchangeable with the tank 25 as a center.
- the flow path 40 rotates around the rotary shaft 22a from the horizontal direction located in the second quadrant to the vertical direction located in the third quadrant, the valve 24 that changes the flow direction as a driving mechanism, the pump 23 that feeds the liquid.
- the rotation mechanism 22 that eliminates bubbles in the culture vessel 20
- cell seeding and culture medium exchange by feeding cell fluid or culture medium into the culture vessel 20 and channel washing with a washing solution can be performed.
- the rotation around the rotation axis 22a from the second quadrant to the third quadrant of the culture vessel base 21 as a holding base on which the culture vessel 20 is installed refers to the culture vessel base 21 from the horizontal position shown in FIG. Can be realized by rotating it downward by the rotation mechanism 22 counterclockwise.
- the drive mechanism is disposed on the drive base 27, and all wiring and mechanisms that cannot be exposed to humidity and the like can be placed inside the drive base 27 and installed in a high humidity environment.
- the drive base 27 has a waterproof connector 31.
- the drive mechanism is operated by connecting the connector 31 to the connector 31. Can do.
- the culture container module 35 which is a second module for feeding the culture container 20 around the tank 25, and the first module for feeding the medium in the refrigerator 14 to the tank 25.
- the medium module 36 is divided into three independent modules: a pump module 37 which is a third module for controlling the flow of gas (direction and flow velocity) for feeding the liquid inside the flow path 40.
- This modularization can improve the optimal arrangement of the drive mechanism and the ease of installation of the flow path.
- the flow paths 40 formed by using the tubes 41 connected from the tank 25 to the first, second, and third modules are respectively designated as the first, second, and third flow path groups. Sometimes called. Note that first, second, and third valve groups each including a valve 24 are formed on the drive base 27 in the middle of the tubes 41 that constitute the first, second, and third flow path groups. .
- the tank 25 serving as the center of each of the first, second, and third modules of this embodiment will be described.
- the tank 25 has an injection tank 65 and a waste liquid tank 66 for the culture container 20. Further, there is a fitting 46 having different roles depending on the height. It is divided into a first fitting 67, a second fitting 68, and a third fitting 69 from the bottom.
- the injection tank 65 side is a liquid supply port to the culture container 20
- the waste liquid tank 66 side is a liquid supply port to the waste liquid bag 63 and the collection bag 64.
- the cell suspension, medium, and washing solution sent from the medium module 36 are injected into the injection tank 65 on the injection tank 65 side, and the liquid waste from the culture vessel 20 is transferred to the waste liquid tank 65 on the waste liquid tank 66 side. It is an inlet to the inside.
- the liquid that can enter the tank 25 has a capacity that does not reach the second fitting 68.
- the third fitting 69 is connected to the pump module 37 and is used for air flow control for liquid feeding in the flow path 40.
- the tank 25 has a taper 47 so that cells do not remain on the wall surface during liquid feeding. By this inclination, it is possible to prevent the cells in the cell suspension 25 from remaining in the tank 25.
- the liquid is fed into the culture container 20 for cell seeding or medium exchange, it can be once warmed to the ambient temperature in the tank 25. It is also possible to install a heater in the injection tank 65.
- the syringe pump 44 includes the motor 70, the ball screw 71, the encoder 72, the drive base 73, the syringe stopper 74, and the fastener 75 in the state shown in FIGS. 7A and 7C.
- Two syringes 43 connected to a valve 76 that makes the gas flow inside the syringe 43 one side are installed on the syringe stopper 74 and the drive base 73 so as to face each other.
- the fastener 75 is pulled up and rotated as shown in FIG. 7D, and the syringe 43 is fixed as shown in FIGS. 7B and 7E.
- the cell suspension or culture medium is sent to the injection tank 65 side of the tank 25 by the syringe pump 44.
- the liquid in the tube 41 is removed through the gas from the filter 45, or a process of returning the liquid to the origin position in the opposite direction is performed to enable accurate liquid delivery next time.
- the solution is fed to the culture vessel 20 by the syringe pump 44 through the valve 24.
- the gas and waste liquid in the culture vessel 20 are sent to the waste liquid tank 66 side in the tank 25.
- the valve 24 of the pump module 37 is switched, and the waste liquid is sent to the waste liquid bag 63 or the recovery bag 64.
- the valve 24 of the pump module 37 is switched, and the cleaning liquid in the cleaning bag 62 is sent to the injection tank 65 side of the tank 25.
- the liquid in the tube 41 is removed through the gas from the filter 45, or a process of returning the liquid to the origin position in the opposite direction is performed to enable accurate liquid delivery next time.
- the cleaning liquid is sent to the waste liquid tank 66 side in the tank 25 so as not to send the washing liquid to the culture vessel 20 via the valve 24.
- the valve 24 of the pump module 37 is switched to send the waste liquid to the waste bag 66. The above operation is repeated a predetermined number of times during the culture period. In the flow path 40, particularly in the culture vessel module 35, the liquid flows in one way.
- FIG. 8A shows the state of the drive base 27 and the culture medium base 32 in which the cell suspension 48 is not injected into the flow path 40
- FIG. 8B shows that the cell suspension 48 is injected into the cell bag 60 of the flow path 40. It shows the state.
- the drive base 27 and the culture medium base 32 in which the flow path 40 is installed are installed in the transport jig 19 before being installed in the automatic culture apparatus 10.
- the transfer jig 19 can install and remove the drive base 27 and the like inside the automatic culture apparatus 10, and after the installation, only the transfer jig 19 can be taken out.
- An empty cell bag 60 is contained in the medium base 32.
- the transfer jig 19 can be placed in a space 77 of class 10,000.
- GMP Good Manufacturing Practice
- the cell direct processing area is only the class 100 space 78 inside the safety cabinet 79 (including the clean bench). Therefore, only the cell bag 60 that has been carefully sterilized with ethanol or the like is placed in the safety cabinet 79.
- the cell suspension 48 processed in the safety cabinet 79 is injected into the cell bag 60 from the injection port 89 with a syringe or the like. After injection, the inlet 89 is sealed to maintain the closed system of the flow path 40.
- the cell bag 60 containing the cell suspension 48 is taken out of the class 100 space 78 and placed in the medium base 32. Since the flow path 40 holds the class 100 space 78 by sterilization in advance, even if the flow path 40 exists in the class 10,000 space 77 as shown in FIG. 8A, the inside of the flow path 40 can hold the class 100 space 78. . After that, even if the automatic culture apparatus 10 is installed, the drive mechanism is not connected to the inside of the flow path 40. Therefore, regardless of the installation space of the automatic culture apparatus 10, the inside of the closed-system flow path 40 is always a class. 100 spaces 78 can be maintained.
- FIG. 9 is a block diagram showing a configuration of a control system circuit for controlling internal devices in the automatic culture apparatus 10 in the present embodiment.
- the control system circuit of the automatic culture apparatus 10 includes an input unit (keyboard, mouse, etc.) 81 for inputting data and instructions, a control unit 82 for controlling each operation of the automatic culture apparatus 10, and the control status to the user.
- a display unit 80 a ROM 85 for storing programs and parameters, a RAM 86 for temporarily storing data and processing results, a memory 83 for performing operations such as a cache for performing operations such as a cache, and communication.
- Unit 84 a sterilization process, a heater, a fan, a carbon dioxide supply, a water supply, and the like, and a drive unit including a rotation mechanism 22 connected to the drive base 27 and an environment holding device 87 using a sensor for monitoring these conditions 59 and a refrigerator control 88 for controlling the environment in the refrigerator.
- the control unit 82 sterilizes the inside of the automatic culture device 10 by the sterilization function of the environment holding device 87 according to the culture preparation program stored in the ROM 85. Then, after the treatment is completed, the culture environment holding treatment is advanced to a temperature of 37 ° C., a 5% carbon dioxide concentration, a humidity of 100%, and a clean environment field. At the same time, the refrigerator control 88 is performed. Then, according to the automatic culture program stored in the ROM 85, the control unit 82 senses the set of the drive base 27 with the position sensor, and when the installation of the connector 31 is sensed, the rotation mechanism 22 and the drive base 27 cause the culture vessel 20 to move.
- the cell culture treatment is performed.
- the display unit 80 and the communication unit 84 can indicate the processing status to the user.
- the display unit 80 and the communication unit 84 indicate the completion to the user.
- the control unit 82 performs the termination process according to the termination program stored in the ROM 85. As described above, a series of cell culture processes by the automatic culture apparatus 10 can be realized.
- a description will be given of an embodiment of a transport device for aseptically supplying a cell solution or the like into a closed system flow path of a cell culture device and a method for injecting a liquid such as a sterile cell solution.
- a transport device for aseptically supplying a cell solution or the like into a closed system flow path of a cell culture device and a method for injecting a liquid such as a sterile cell solution.
- FIG. 8A shows the state of the drive base 27 and the culture medium base 32 in which the cell suspension 48 is not injected into the flow path 40
- FIG. The state where the suspension 48 is being injected is shown.
- 8C shows the configuration of the transport jig 19 having a plurality of holders, which is the transport apparatus according to the present embodiment.
- the automatic culture apparatus is used by using the transport jig 19. A method for installing the flow path 40 and the like will be described.
- 8D shows a state before the flow path 40 and the like are installed in the automatic culture apparatus 10
- FIG. 8E shows a state where the flow path 40 and the like are installed in the automatic culture apparatus 10
- FIG. 8F shows the automatic culture apparatus.
- 109 shows a state after the holding base including the flow path 40 is installed.
- FIG. 8C shows a detailed configuration of the transport jig 19 including a plurality of holders of the present embodiment.
- 8A shows a side view
- FIG. 8C shows a top view of FIG. 8B.
- the lift 98 which is a third holder that holds the drive base itself, includes a conveyance jig side pin 90 that is a fixing portion, a second holder 92 that holds the culture vessel base, and a first holder that holds the culture base. 93, and the drive base 27, the culture vessel base 21, and the culture medium base 32 described above can be installed respectively.
- the lift 98 can be moved in the vertical movement direction 96 by a handle 99 that functions as a vertical drive mechanism.
- a counterweight 97 that functions as a center-of-gravity stabilizing portion is installed in the conveying jig 19, and when a heavy object is installed on the lift 98, stability can be maintained when stationary or moving.
- an electric vertical drive mechanism such as a motor may be employed.
- the transfer jig 19 that is in the state after the processing described in FIG. 8B and is located on the floor surface 200 that is the installation surface of the cell culture device 10 includes the culture vessel base 21, the drive base 27, The medium base 32 and the flow path 40 are installed, and the height is adjusted to a height that can be inserted into the automatic culture apparatus 10 by a height adjusting mechanism such as a handle 99.
- the automatic culture apparatus 10 has a drive base guide 94 and a device side pin 91 that adjust the position as a guide portion when the drive base 27 is inserted into the cell culture chamber 13, and when the medium base 32 is inserted into the refrigerator 14, There is a drive base guide 95 that adjusts the position as a guide portion.
- the position is adjusted by the drive base guide 94 and the medium base guide 95 which are guide portions.
- the position adjustment for installing the culture vessel base 21 on the rotation mechanism 22 is also performed at the same time.
- a state in which the second holder 92 that holds the culture vessel base and the culture vessel base claw 100 shown in FIG. 1 are separated, a state in which the pin 90 is separated from the drive base 27 and is connected to the pin 91, and a medium base is held.
- the handle 98 moves the lift 98, which is the third holder for holding the drive base 27, in the movement direction 96.
- the culture vessel base 21 is moved to the rotation mechanism 22 and the drive base 27 is cell culture as shown in FIG. 8F.
- the culture medium base 32 is installed in the refrigerator 13 at the same time.
- the seal 33 is installed on the claw 34 (see FIG. 4A).
- the cell culture chamber door 11, the refrigerator door 12, and the intermediate chamber door 17 are closed to perform automatic culture processing. 8F, 8E, and 8D after the completion of the automatic culture process, the culture vessel base 21, the drive base 27, and the culture medium base 32 can be taken out from the automatic culture apparatus 11 by the transport jig 19 held by the corresponding holders. it can.
- the present invention is not limited to the above-described embodiments, and includes various modifications. As described above, various modifications such as using a tube pump instead of a syringe pump as a driving pump are possible. Further, the above-described embodiments have been described in detail for better understanding of the present invention, and are not necessarily limited to those having all the configurations described above.
- each configuration, function, processing unit, and the like of the control mechanism of the automatic culture apparatus described above may be realized by hardware by designing a part or all of them with, for example, an integrated circuit.
- the program may be realized by creating a program that is executed by a CPU that is a processing unit for all or part of the program.
- Item 1 A liquid injection method for injecting a cell solution into a cell bag to be conveyed to a cell culture device, A plurality of bags including the cell bag; and a first flow path group for supplying the medium and cells from a part of the plurality of bags and discharging the medium and cells to the other part of the plurality of bags.
- a culture medium base comprising: a culture container for culturing the cells; a culture container base provided with a second flow path group for supplying the culture medium and cells to the culture container and discharging the culture medium and cells from the culture container; Direct cell processing of a transport device including a holder that holds a drive base including a pump unit that controls the amount of the culture medium and the liquid feeding of the cells inside the channels in the first and second channel groups. Transport to the vicinity of the area, From the medium base, the cell bag connected to the flow path is moved to the cell direct treatment area, and in the cell direct treatment area, the cells are injected into the cell bag, After the cell bag after injecting the cells is placed on the medium base, it is transported to the cell culture device.
- a liquid injection method characterized by the above.
- the liquid injection method according to Item 1 wherein The cell bag is provided with an injection port for injecting the cell, and the cell is injected through the injection port.
- Item 3 Item 2.
- Item 4 Item 2.
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Abstract
Description
本発明の他の目的は、上記の課題を解決するため、細胞培養装置の閉鎖系流路内へ細胞液等を無菌的に供給するための搬送装置、及び無菌的な細胞液等の液体注入方法を提供することにある。
図1は、第一の実施例に係る自動培養装置10の全体概略図である。図2は自動培養装置10の正面図である。図3は自動培養装置10の側面図である。図4A及び4Bは細胞培養室13と冷蔵庫14間のシール33に関する概略図である。図5は流路40の構成に関する概略図である。図6は流路40の構成部品の一つであるタンク25に関する概略図である。図7A、7B、7C、7D、及び7Eは流路40の構成部品の一つであるポンプ23に関する概略図である。図8A,及び8Bは流路40内へ無菌的な細胞液や培地の供給に関する概略図である。図9は自動培養装置10を動作させるための回路を示すブロック図である。
図1、図2、図3及び図4A、図4Bを用いて、自動培養装置10の全体構成について説明する。自動培養装置10は基本的な構成要素として、細胞培養室13、冷蔵庫14、制御部15、中間室16によって構成されている。以下、それぞれの基本構成について説明する。
図5、図6、及び図7A-7Eを用いて、本実施例の細胞培養装置における流路40の構成とその駆動方法について説明する。図5は流路40とタンク25と駆動ベース27の全体構成を示した概略図である。図6はタンク25の構成を示した正面図である。図7Aはシリンジ43とポンプ23が分離した状態の上面図を示し、図7Bはシリンジ43とポンプ23が一体になった状態の上面図を示し、図7Cはシリンジ43とポンプ23が分離した状態の側面図を示し、図7Dはシリンジ43をポンプ23にセットしている状態の側面図を示し、図7Eはシリンジ43とポンプ23が一体でシリンジポンプ44となった状態の側面図を示している。シリンジ43は同一軸上を並進することができる。なお、本実施例において、駆動用のポンプとしてシリンジポンプを用いて説明するが、駆動用のポンプとして、チューブポンプなどの他のポンプを用いることもできる。
、培養容器20が設置される保持ベースとしての培養容器ベース21の第二象限から第三象限への回転軸22aの周りでの回転とは、図2に見る水平位置から培養容器ベース21を回転機構22により、反時計方向回りに下方に回転させることで実現できる。
<培養容器への細胞懸濁液もしくは培地の注入に関する一連の動作>
以上の各部の構成に基づき、本実施例の自動培養装置の一連の細胞培養動作の一例について説明する。細胞懸濁液もしくは培地をシリンジポンプ44でタンク25の注入タンク65側へ送液する。所定量入ったらフィルタ45から気体を通してチューブ41内の液体を排除したり、また逆向きに送り液体を原点位置まで戻す処理を施して、次回も正確に送液を可能とする。注入タンク65で細胞懸濁液もしくは培地を温める。弁24を介して、シリンジポンプ44で培養容器20へ送液する。その際、培養容器20内の気体や廃液はタンク25内の廃液タンク66側に送られる。ポンプモジュール37の弁24を切り替え、廃液を廃液バッグ63もしくは回収バック64に送る。ポンプモジュール37の弁24を切り替え、洗浄バッグ62内の洗浄液をタンク25の注入タンク65側へ送液する。所定量入ったらフィルタ45から気体を通してチューブ41内の液体を排除したり、また逆向きに送り液体を原点位置まで戻す処理を施して、次回も正確に送液を可能とする。弁24を介して、培養容器20へ洗浄液を送液しないようタンク25内の廃液タンク66側に送る。ポンプモジュール37の弁24を切り替え、廃液を廃液バッグ66に送る。以上の動作を培養期間中、所定の回数繰り返す。流路40内部特に培養容器モジュール35では一方通行で液が流れる。それにより、廃液が培養容器20内の培養空間と培地バッグ61類の回路に戻らずに細胞には常に清潔で新鮮な培地が供給されるため、菌等が入らず清浄環境を保持することを可能にする。
<閉鎖系流路内部への液体注入>
図8A,8Bにおいて、本実施例における閉鎖系構造を有した流路40内へ細胞懸濁液48の無菌的な注入方法について説明する。図8Aは流路40内部に細胞懸濁液48を注入していない駆動ベース27と培地ベース32の状態を示し、図8Bは流路40の細胞バッグ60内部に細胞懸濁液48を注入している状態を示す。
。細胞懸濁液48が入った細胞バッグ60をクラス100空間78の外に出し、培地ベース32の中に入れる。事前に流路40は滅菌処理により、クラス100空間78を保持しているため、流路40は図8Aのようにクラス10,000空間77で存在しても、その内部はクラス100空間78を保持できる。この後、自動培養装置10内へ設置をしても、駆動機構は流路40内部とは接続しないため、自動培養装置10の設置空間に関わらず、閉鎖系構造の流路40内部は常にクラス100空間78を保持することが可能である。
図9は、本実施例における自動培養装置10における内部機器を制御するための制御系回路の構成を示すブロック図である。
図8A~図8Fを用いて、実施例2における、搬送装置、閉鎖系流路内部への液体注入方法の具体例を説明する。具体的には、一例として、閉鎖系構造を有した流路40内へ細胞懸濁液48の無菌的な注入方法について説明する。細胞懸濁液48以外の液体についても同様の注入法を利用することが出来る。
*項1
細胞培養装置に搬送する細胞バッグに細胞液を注入する液体注入方法であって、
前記細胞バッグを含む複数のバッグと、前記複数のバッグの一部から前記培地及び細胞を供給し、前記複数のバッグの他の一部へ前記培地及び細胞を排出する第一の流路群を備える培地ベースと、前記細胞を培養する培養容器と、前記培養容器に前記培地及び細胞を供給し、前記培養容器から前記培地及び細胞を排出する第二の流路群を備える培養容器ベースと、前記第一、第二の流路群内の流路の内部における前記培地及び細胞の送液の量を制御するポンプ部を備える駆動ベースを保持する保持具を備えた搬送装置を、細胞直接処理区域近傍に搬送し、
前記培地ベースから、前記流路に接続された前記細胞バッグを前記細胞直接処理区域に移動し、前記細胞直接処理区域にて、前記細胞バッグに前記細胞を注入し、
前記細胞を注入後の前記細胞バッグを前記培地ベースに設置した後、前記細胞培養装置に搬送する、
ことを特徴とする液体注入方法。
*項2
項1に記載の液体注入方法であって、
前記細胞バッグは、前記細胞を注入する注入口を備え、当該注入口を介して前記細胞を注入することを特徴とする液体注入方法。
*項3
項1に記載の液体注入方法であって、
前記搬送装置を前記細胞培養装置に搬送した後、前記保持具から、前記培地ベース、前記培養容器ベース、及び前記駆動ベースを取り外し、前記細胞培養装置内に設置することを特徴とする液体注入方法。
*項4
項1に記載の液体注入方法であって、
前記細胞直接処理区域はクラス100空間であり、前記細胞培養装置が設置される場所は、クラス10000空間であることを特徴とする液体注入方法。
11 細胞培養室扉
12 冷蔵庫扉
13 細胞培養室
14 冷蔵庫
15 制御部
16 中間室
17 中間室扉
18 冷却器
19 搬送治具
20 培養容器
21 培養容器ベース
22 回転機構
23 ポンプ
24 弁
25 タンク
27 駆動ベース
28 顕微鏡
29 ステージ
30 コネクタボード
31 コネクタ
32 培地ベース
33 シール
34 ツメ
35 培養容器モジュール(第2のモジュール)
36 培地モジュール(第1のモジュール)
37 ポンプモジュール(第3のモジュール)
40 流路
41 チューブ
42 溝
43 シリンジ
44 シリンジポンプ
45 フィルタ
46 フィッティング
47 テーパー
48 細胞懸濁液
50 ファン(中間室)
51 フィルタ(中間室)
52 ファン(制御部)
53 吸気用フィルタ
54 排気用フィルタ
55 空気の流れ(中間室)
56 空気の流れ(制御部)
57 制御パネル(制御部)
59 駆動部
60 細胞バッグ
61 培地バッグ
62 洗浄液バッグ
63 廃液バッグ
64 回収バッグ
65 注入タンク
66 廃液タンク
67 第1のフィッティング
68 第2のフィッティング
69 第3のフィッティング
70 モーター
71 ボールねじ
72 エンコーダ
73 駆動台
74 シリンジストッパ
75 留め具
76 弁
77 クラス10,000の空間
78 クラス100の空間
79 安全キャビネット
80 表示部
81 入力部
82 制御部
83 メモリ
84 通信部
85 ROM
86 RAM
87 環境保持装置
88 冷蔵庫制御
89 注入口
90 ピン(搬送治具側)
91 ピン(細胞培養装置側)
92 培養容器ベース保持具
93 培地ベース保持具
94 駆動ベースガイド
95 培地ベースガイド
96 移動方向(上下)
97 カウンターウェイト(おもり)
98 リフト
99 ハンドル
100 培養容器ベースツメ
200 床面
Claims (16)
- 培地を用いて細胞を培養する細胞培養装置であって、
前記培地及び細胞を有する複数のバッグと、前記複数のバッグの一部から前記培地及び細胞を供給し、前記複数のバッグの他の一部へ前記培地及び細胞を排出する第一の流路群を有する第一のモジュールと、
前記細胞を培養する培養容器と、前記培養容器に前記培地及び細胞を供給し、前記培養容器から前記培地及び細胞を排出する第二の流路群を有する第二のモジュールと、
前記第一の流路群内の流路から流入する前記培地及び細胞を保持し、保持した前記培地及び細胞を、前記培養容器へ供給するため、前記第二の流路群内の流路へ流出する注入タンクと、前記培養容器から排出され、前記第二の流路群内の流路から流入する前記培地及び細胞を保持し、保持した前記培地及び細胞を、前記複数のバッグの他の一部へ排出するため、前記第一の流路群内の流路へ流出する廃液タンクを有するタンク部と、
前記第一、第二の流路群内の流路の内部における前記培地及び細胞の送液の量と、前記タンク部の内部に保持される前記培地及び細胞の量を制御するポンプ部を有する第三のモジュールと、を備える
ことを特徴とする細胞培養装置。 - 請求項1に記載の細胞培養装置であって、
前記第一のモジュールと、前記第二のモジュールと、前記第三のモジュールとを載置する保持ベースを有する
ことを特徴とする細胞培養装置。 - 請求項1に記載の細胞培養装置であって、
前記第一のモジュールと、前記第二のモジュールと、前記第三のモジュールとが、前記細胞培養装置に着脱可能である
ことを特徴とする細胞培養装置。 - 請求項2に記載の細胞培養装置であって、
前記保持ベースは、前記細胞培養装置に着脱可能である
ことを特徴とする細胞培養装置。 - 請求項2に記載の細胞培養装置であって、
前記保持ベースは、前記複数のバッグ、前記培養容器、前記ポンプ部をそれぞれ保持する、培地ベース、培養容器ベース、駆動ベースからなり、前記駆動ベースは、前記第一、第二流路群内の前記培地及び細胞の流量と流れる方向とを切り替える弁を更に保持する
ことを特徴とする細胞培養装置。 - 請求項5に記載の細胞培養装置であって、
前記ポンプ部及び前記弁を制御する制御部を更に有する
ことを特徴とする細胞培養装置。 - 培地を用いて細胞を培養する細胞培養装置であって、
前記培地及び細胞を有する複数のバッグと、前記複数のバッグの一部から前記培地及び細胞を供給し、前記複数のバッグの他の一部へ前記培地及び細胞を排出する第一の流路群を有し、保持ベースに保持される第一のモジュールと、
前記細胞を培養する培養容器と、前記培養容器に前記培地及び細胞を供給し、前記培養容器から前記培地及び細胞を排出する第二の流路群を有し、前記保持ベースに保持される第二のモジュールと、
前記第一の流路群内の流路から流入する前記培地及び細胞を保持し、保持した前記培地及び細胞を、前記培養容器へ供給するため、前記第二の流路群内の流路へ流出する注入タンクと、前記培養容器から排出され、前記第二の流路群内の流路から流入する前記培地及び細胞を保持し、保持した前記培地及び細胞を、前記複数のバッグの他の一部へ排出するため、前記第一の流路群内の流路へ流出する廃液タンクを有するタンク部と、
前記第一、第二の流路群内の流路の内部における前記培地及び細胞の送液の量と、前記タンク部の内部に保持される前記培地及び細胞の量を制御するポンプ部を有し、前記保持ベースに保持される第三のモジュールと、
を備えることを特徴とする細胞培養装置。 - 請求項7に記載の細胞培養装置であって、
前記保持ベースは、前記複数のバッグ、前記培養容器、前記ポンプ部をそれぞれ保持する、培地ベース、培養容器ベース、駆動ベースからなり、前記培地ベース、前記培養容器ベース、及び前記駆動ベースは、前記細胞培養装置に着脱可能である
ことを特徴とする細胞培養装置。 - 請求項7に記載の細胞培養装置であって、
前記第2の流路群は、前記培養容器に形成された供給口と排出口に接続されることを特徴とする細胞培養装置。 - 請求項7に記載の細胞培養装置であって、
前記ポンプ部はシリンジポンプを備えることを特徴とする細胞培養装置。 - 細胞培養装置に培地及び細胞の複数のバッグを搬送及び着脱可能な搬送装置であって、
前記複数のバッグと、前記複数のバッグの一部から前記培地及び細胞を供給し、前記複数のバッグの他の一部へ前記培地及び細胞を排出する第一の流路群を備える培地ベースを保持する第一の保持具と、
前記細胞を培養する培養容器と、前記培養容器に前記培地及び細胞を供給し、前記培養容器から前記培地及び細胞を排出する第二の流路群を備える培養容器ベースを保持する第二の保持具と、
前記第一、第二の流路群内の流路の内部における前記培地及び細胞の送液の量を制御するポンプ部を備える駆動ベースを保持する第三の保持具と、を備えることを特徴とする搬送装置。 - 請求項11に記載の搬送装置であって、
前記第三の保持具は、前記駆動ベースが保持される位置を固定する固定部を有することを特徴とする搬送装置。 - 請求項11に記載の搬送装置であって、
前記第一、第三の保持具は、前記細胞培養装置内に形成された案内部に沿って、前記細胞培養装置への前記培地ベース及び前記駆動ベースの着脱を行う形状を有することを特徴とする搬送装置。 - 請求項11に記載の搬送装置であって、
前記第一、第二及び第三の保持具と、当該搬送装置の接地面との間の距離を変化させる駆動機構を更に備え、
前記駆動機構は、
当該搬送装置が前記細胞培養装置内に挿入された場合、前記接地面との間の距離を変化させ、前記第一、第二及び第三の保持具から、前記培地ベース、前記培養容器ベース、前記駆動ベースを取り外し、前記細胞培養装置内に設置させることを特徴とする搬送装置。 - 請求項11に記載の搬送装置であって、
当該搬送装置の立脚を安定させる重心安定部を有することを特徴とする搬送装置。 - 請求項11に記載の搬送装置であって、
前記第二の保持具は前記回転機構を介して第三の保持具に接続されていることを特徴とする搬送装置。
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KR101589356B1 (ko) | 2016-01-27 |
US9951305B2 (en) | 2018-04-24 |
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