WO2021112172A1

WO2021112172A1 - Cleaning agent, and cleaning method and cleaning device using cleaning agent

Info

Publication number: WO2021112172A1
Application number: PCT/JP2020/045015
Authority: WO
Inventors: 毅小川; 勝宏秋山; 佳松永; 雄貴関矢; 慎介三木
Original assignee: セントラル硝子株式会社
Priority date: 2019-12-06
Filing date: 2020-12-03
Publication date: 2021-06-10
Also published as: TW202128973A

Abstract

Provided are a cleaning agent having adequate cleaning power and a cleaning method and a cleaning device that use a cleaning agent. The cleaning agent according to one embodiment contains a fluorine-based alcohol as a compound that inactivates viruses. The cleaning agent according to one embodiment contains a fluorine-based alcohol as a compound that kills bacteria. In the cleaning agent according to one embodiment, the fluorine-based alcohol is represented by the general formula RfCH2OH or RfRf'CHOH; Rf and Rf' represent a C1-10 perfluoroalkyl group, and Rf and Rf' may be the same or different from each other.

Description

Detergent, cleaning method using detergent and cleaning equipment

The present disclosure relates to a cleaning agent, a cleaning method using a cleaning agent, and a cleaning device.

Social interest in infectious diseases caused by viruses such as influenza virus and norovirus is high, and demand for antiviral products is expanding in various fields in search of a comfortable and clean living environment. Especially in the medical field where cleanliness is required, infection control is very important, and reliable implementation will lead to the protection of the safety of patients and medical staff. For example, medical instruments used for patients are cleaned, disinfected, and sterilized according to the purpose of use of the instruments. These treatment methods are selected based on the risk of infection when using medical devices. With either method, thorough cleaning is of utmost importance. This is because poor cleaning can cause subsequent poor disinfection and sterilization.

Cleaning of medical equipment is roughly divided into three types: manual cleaning (including immersion cleaning), ultrasonic cleaning, and washer-disinfector. The cleaning method is selected in consideration of the facility environment and the characteristics of the medical device. Cleaning agents for medical equipment are mainly classified into three types: alkaline cleaning agents, acidic cleaning agents, and enzyme cleaning agents. Alkaline cleansers have excellent detergency, but have a strong effect on the material and skin. Acid cleaners are suitable for cleaning inorganic substances (rust, scale, scale, oxide film, etc.), but are highly corrosive to metals. Enzyme-based cleaning agents have little effect on the material, but their cleaning power is inferior. Therefore, it is necessary to select an appropriate cleaning agent depending on the characteristics of the medical device and the cleaning method (mechanical cleaning, manual cleaning, immersion cleaning).

Cleaning is important in various fields, and it is necessary to select an appropriate cleaning agent depending on the object to be cleaned and the object to be removed from the object. For example, it is known that a cleaning agent containing 1,1,1,3,3,3-hexafluoro-2-propanol is used in the semiconductor manufacturing process. Patent Document 1 describes side walls of STI, metal gates, contact holes, via holes, capacitors, metal wiring, etc. by utilizing the polymer solubility of 1,1,1,3,3,3-hexafluoro-2-propanol. For peeling of the polymer remaining in the polymer, for removing the resist residue after ion implantation, for peeling the adhered polymer after dry etching in the single damascene or dual damascene process, or for cleaning after CMP in the single damascene or dual damascene process. It is disclosed to use a cleaning composition containing 1,1,1,3,3,3-hexafluoro-2-propanol. Patent Document 2 describes that 1,1,1,3,3,3-hexafluoro-2-propanol is mixed with water in an arbitrary ratio to clean and dry semiconductor wafers in each lithography process. , 1,1,3,3,3-hexafluoro-2-propanol vapor is disclosed.

Japanese Unexamined Patent Publication No. 2015-108041 Japanese Unexamined Patent Publication No. 3-106024

It is an object of the present disclosure to provide a cleaning agent having sufficient detergency, a cleaning method using the cleaning agent, and a cleaning device.

As a result of diligent studies by the present inventors, the

fluoroalcohols

1,1,1,3,3,3-hexafluoro-2-propanol (HFIP), 2,2,2-trifluoroethanol (TFE), And 2,2,3,3,3-pentafluoro-1-propanol were found to have virus inactivating and bactericidal effects.

This disclosure includes the following aspects.

[1] A cleaning agent containing a fluoroalcohol as a compound that inactivates a virus.

[2] Fluoro-based alcohols are represented by the general formula RfCH ₂ OH or RfRf'CHOH, Rf and Rf'represent a perfluoroalkyl group having 1 to 10 carbon atoms, and Rf and Rf'are different or the same. A cleaning agent.

[3] Fluorine-based alcohols are 1,1,1,3,3,3-hexafluoro-2-propanol, 2,2,2-trifluoroethanol and 2,2,3,3,3-pentafluoro-. A cleaning agent that is at least one selected from the group consisting of 1-propanol.

[4] A cleaning agent having a fluorine-based alcohol concentration of 0.1% by mass or more when acting on a virus.

[5] A cleaning agent further containing a solvent.

[6] The virus is at least one cleaning agent selected from the group consisting of influenza virus, hepatitis C virus, rotavirus, norovirus, adenovirus and enterovirus.

[7] A cleaning agent used for cleaning medical instruments.

[8] A cleaning agent used for cleaning soil.

[9] A cleaning method involving the action of a fluoroalcohol on a virus.

[10] Fluoro-based alcohols are represented by the general formula RfCH ₂ OH or RfRf'CHOH, Rf and Rf'show perfluoroalkyl groups having 1 to 10 carbon atoms, and Rf and Rf' are different or the same as each other. Cleaning method.

[11] Fluorine-based alcohols are 1,1,1,3,3,3-hexafluoro-2-propanol, 2,2,2-trifluoroethanol and 2,2,3,3,3-pentafluoro-. A cleaning method that is at least one selected from the group consisting of 1-propanol.

[12] A cleaning method in which the concentration of fluorinated alcohol when acting on a virus is 0.1% by mass or more.

[13] A cleaning method in which a fluoroalcohol is diluted with a solvent.

[14] A cleaning method in which the virus is at least one selected from the group consisting of influenza virus, hepatitis C virus, rotavirus, norovirus, adenovirus and enterovirus.

[15] A cleaning method used for cleaning medical instruments.

[16] A cleaning method used for cleaning soil.

[17] A cleaning device including a cleaning agent supply device that supplies a cleaning agent containing a fluoroalcohol as a compound that inactivates a virus.

[18] Fluorine-based alcohols are represented by the general formula RfCH ₂ OH or RfRf'CHOH, Rf and Rf'represent a perfluoroalkyl group having 1 to 10 carbon atoms, and Rf and Rf'are different or the same. A cleaning device.

[19] Fluorine-based alcohols are 1,1,1,3,3,3-hexafluoro-2-propanol, 2,2,2-trifluoroethanol and 2,2,3,3,3-pentafluoro-. A cleaning device that is at least one selected from the group consisting of 1-propanol.

[20] A cleaning device in which the concentration of fluorinated alcohol when acting on a virus is 0.1% by mass or more.

[21] A cleaning device in which a fluorine-based alcohol is diluted with a solvent.

[22] The virus is at least one cleaning device selected from the group consisting of influenza virus, hepatitis C virus, rotavirus, norovirus, adenovirus and enterovirus.

[23] A cleaning device used for cleaning medical instruments.

[24] A cleaning device used for cleaning soil.

[25] A cleaning agent containing a fluoroalcohol as a compound that kills bacteria.

[26] Fluoro-based alcohols are represented by the general formula RfCH ₂ OH, or RfRf'CHOH, where Rf and Rf'represent perfluoroalkyl groups having 1 to 10 carbon atoms, and Rf and Rf'are different or the same. A cleaning agent.

[27] Fluorine-based alcohols are 1,1,1,3,3,3-hexafluoro-2-propanol, 2,2,2-trifluoroethanol and 2,2,3,3,3-pentafluoro-. A cleaning agent that is at least one selected from the group consisting of 1-propanol.

[28] A cleaning agent having a fluorine-based alcohol concentration of 0.1% by mass or more when acting on bacteria.

[29] A cleaning agent further containing a solvent.

[30] A cleaning agent used for cleaning medical instruments.

[31] Detergent used for sterilization.

[32] A cleaning agent used for cleaning soil.

[33] A cleaning method comprising allowing a fluorinated alcohol to act on bacteria.

[34] Fluoro-based alcohols are represented by the general formula RfCH ₂ OH, or RfRf'CHOH, Rf and Rf'show perfluoroalkyl groups having 1 to 10 carbon atoms, and Rf and Rf'are different or the same. Cleaning method.

[35] Fluorine-based alcohols are 1,1,1,3,3,3-hexafluoro-2-propanol, 2,2,2-trifluoroethanol and 2,2,3,3,3-pentafluoro-. A cleaning method that is at least one selected from the group consisting of 1-propanol.

[36] A cleaning method in which the concentration of fluorinated alcohol when acting on bacteria is 0.1% by mass or more.

[37] A cleaning method in which a fluoroalcohol is diluted with a solvent.

[38] A cleaning method used for cleaning medical instruments.

[39] A cleaning method used for cleaning soil.

[40] A cleaning device including a cleaning agent supply device that supplies a cleaning agent containing a fluoroalcohol as a compound that kills bacteria.

[41] Fluoro-based alcohols are represented by the general formula RfCH ₂ OH or RfRf'CHOH, Rf and Rf'show perfluoroalkyl groups having 1 to 10 carbon atoms, and Rf and Rf'are different or the same. A cleaning device.

[42] Fluorine-based alcohols are 1,1,1,3,3,3-hexafluoro-2-propanol, 2,2,2-trifluoroethanol and 2,2,3,3,3-pentafluoro-. A cleaning device that is at least one selected from the group consisting of 1-propanol.

[43] A cleaning device in which the concentration of fluorinated alcohol when acting on bacteria is 0.1% by mass or more.

[44] A cleaning device in which the fluorine-based alcohol is diluted with a solvent.

[45] A cleaning device used for cleaning medical instruments.

[46] A cleaning device used for cleaning soil.

According to one embodiment of the present disclosure, it is an object of the present invention to provide a cleaning agent having sufficient detergency, a cleaning method using the cleaning agent, and a cleaning device.

It is sectional drawing explaining the structure of the cleaning apparatus which concerns on one Example of this disclosure. It is a figure which shows the evaluation of the virus inactivation of the cleaning agent which concerns on one Example of this disclosure. It is a figure which shows the evaluation of the virus inactivation of the cleaning agent which concerns on one Example of this disclosure. It is a figure which shows the evaluation of the virus inactivation of the cleaning agent which concerns on one Example of this disclosure. It is a figure which shows the evaluation of the virus inactivation of the cleaning agent which concerns on one Example of this disclosure. It is a figure which shows the evaluation of the virus inactivation of the cleaning agent which concerns on one Example of this disclosure. It is a figure which shows the evaluation of the virus inactivation of the cleaning agent which concerns on one Example of this disclosure. It is a figure which shows the evaluation of the virus inactivation of the cleaning agent which concerns on one Example of this disclosure. It is a figure which shows the evaluation of the virus inactivation of the cleaning agent which concerns on one Example of this disclosure. It is a figure which shows the evaluation of the virus inactivation of the cleaning agent which concerns on one Example of this disclosure. It is a figure which shows the evaluation of the virus inactivation of the cleaning agent which concerns on one Example of this disclosure. It is a figure which shows the evaluation of the bactericidal property of the cleaning agent which concerns on one Example of this disclosure. It is a figure which shows the evaluation of the bactericidal property of the cleaning agent which concerns on one Example of this disclosure.

[Washing soap]
The compound contained in the cleaning agent of the present disclosure is a fluorinated alcohol. Fluorine-based alcohols are suitable as compounds that inactivate viruses.

In one embodiment, the cleaning agent contains a fluorinated alcohol as an active ingredient. As used herein, the term "cleaning" means removing contaminants adhering to an object from the object. In addition, "contaminants" include, for example, proteins derived from living organisms such as blood, body fluids, fats, prion proteins (PrP) and infectious amyloid, organic substances such as cell tissues, microorganisms, viruses, bacteria and the like. Say. In one embodiment, the cleaning agent contains two or more selected from fluoroalcohols.

In one embodiment, the fluorinated alcohol of the present disclosure is represented by the following general formula (1) or (2).

RfCH ₂ OH ・・・ (1)
RfRf'CHOH ・・・ (2)

In the general formula, Rf and Rf'represent a perfluoroalkyl group having 1 to 10 carbon atoms.

Among the compounds represented by the general formula (1) or (2), 1,1,1,3,3,3-hexafluoro-2-propanol (hereinafter, may be referred to as HFIP), 2, 2,2-Trifluoroethanol (hereinafter, may be referred to as TFE) and 2,2,3,3,3-pentafluoro-1-propanol can be exemplified. Further, in one embodiment, the fluorinated alcohol of the present disclosure contains 1,1,1-trifluoro-2-propanol (hereinafter, may be referred to as TFIPL). Here, TFIPL is a racemate (hereinafter, may be referred to as TFIPL (racemic)), an S-form (hereinafter, may be referred to as S-TFIPL), and an R-form (hereinafter, may be referred to as R-TFIPL). It may be any aspect of). In particular, HFIP and TFIPL are suitable as compounds that inactivate viruses.

In the present specification, the virus includes both a virus having an envelope (enveloped virus) and a virus having no envelope (non-enveloped virus), and a virus having DNA as a genome (DNA virus) and RNA. Any virus with (RNA virus) is targeted. In the present specification, the virus can exemplify, but is not limited to, influenza virus, hepatitis C virus, rotavirus, norovirus, adenovirus, enterovirus and the like. For example, viral hepatitis, viral meningitis, viral gastroenteritis, viral conjunctivitis, acquired immunodeficiency syndrome (AIDS), adult T-cell leukemia, Ebola hemorrhagic fever, yellow fever, cold syndrome, cytomegalovirus infection, severe Acute respiratory syndrome (SARS), Middle Eastern respiratory syndrome (MERS), progressive multifocal leukoencephalopathy, varicella / herpes zoster, simple herpes, limb mouth disease, dengue fever, Japanese encephalitis, infectious erythema, infectious mononuclear bulb Symptoms, natural hemorrhoids, wind rash, acute gray-white myelitis (porio), measles, pharyngeal conjunctivitis (pool fever), Marburg hemorrhagic fever, nephropathy hemorrhagic fever, lassa fever, epidemic parotid inflammation, Westnile fever, herpanguina , Viruses that are the causative agents of viral infections such as Chikungunia fever can be exemplified. In addition, a substitute virus for the above-mentioned virus can be exemplified. Furthermore, viruses that infect animals other than humans include rabies virus and classical swine fever virus.

In one embodiment, the cleaning agent may contain the fluorinated alcohol of the present disclosure and a solvent. In one embodiment, the cleaning agent may contain a compound represented by the general formula (1) or (2) and a solvent. Examples of the solvent capable of diluting the compound represented by the general formula (1) or (2) include water, physiological saline, borate buffer, phosphate buffer (for example, phosphate buffered physiological saline (also referred to as PBS)). Examples thereof include acetic acid buffer, Tris buffer, HEPES buffer, methanol, ethanol, isopropanol, acetone, toluene, dimethyl sulfoxide, ethylene glycol, diethylene glycol and propylene glycol. These may be one or more, but are not limited thereto. In one embodiment, the cleaning agent comprises a compound represented by the general formula (1) or (2) and the above solvent. HFIP is a colorless and transparent liquid having a melting point of -3.3 ° C and a boiling point of 58.6 ° C. TFE is a colorless and transparent liquid having a melting point of -45.0 ° C. and a boiling point of 78.0 ° C. TFIPL is a colorless and transparent liquid having a melting point of −78 ° C. and a boiling point of 22 ° C. Since HFIP, TFE, and TFIPL are soluble in most solvents, they can be adjusted to any concentration by diluting with the solvent. In one embodiment, the content (% by mass) of the solvent contained in the cleaning agent of the present disclosure is 0% by mass or more and 99.9% by mass or less with respect to the mass of the cleaning agent from the viewpoint of detergency. It is also preferable, 0% by mass or more and 99% by mass or less, 0% by mass or more and 92% by mass or less is particularly preferable, and 0% by mass or more and 71% by mass or less is further preferable.

In one embodiment, the cleaning agent may contain additives in addition to the fluorinated alcohol and solvent of the present disclosure. In one embodiment, the cleaning agent may contain an additive in addition to the compound and solvent represented by the general formula (1) or (2). Additives that can be added to detergents include surfactants, enzymes, chelating agents, enzyme stabilizers, blood coagulation inhibitors, metal corrosion inhibitors, low molecular weight polyols, cleaning aids (builders), defoamers, and pH. Conditioners, fragrances, colorants, antioxidants, preservatives, bleaching agents, bleaching activators, corrosion inhibitors, dispersants, thickeners, viscosity regulators, etc., but are not limited to these. .. The cleaning agent may contain one or more additives. In one embodiment, the cleaning agent can further improve the cleaning power by containing the compound represented by the general formula (1) or (2), the solvent, and the above-mentioned additive.

Surfactants (A) include nonionic surfactants (A-1), anionic surfactants (A-2), cationic surfactants (A-3), and amphoteric surfactants (A-). 4) and biosurfactants (A-5) are included.

Examples of the nonionic surfactant (A-1) include an alkylene oxide-added nonionic surfactant (A-1-1) and a polyhydric alcohol-type nonionic surfactant (A-1-2). Can be mentioned.

As the alkylene oxide adduct nonionic surfactant (A-1-1), a higher alcohol (8 to 18 carbon atoms) alkylene (2 to 4 carbon atoms, preferably 2) oxide adduct (1 active hydrogen) Per addition mole number 1 to 30), alkyl (carbon number 1 to 12) phenol ethylene oxide (hereinafter, ethylene oxide may be referred to as EO) adduct (addition mole number 1 to 30), higher amine (carbon) Numbers 8 to 22) alkylene (2 to 4 carbon atoms, preferably 2) oxide adduct (1 to 40 moles added per active hydrogen), fatty acid (8 to 18 carbon atoms) EO adduct (active hydrogen) 1 to 60 moles added per piece), polypropylene glycol (200 to 4000 molecular weight) EO adduct (1 to 50 moles added per active hydrogen), polyoxyethylene (3 to 30 repeating units) alkyl (6 to 20 carbon atoms) Allyl ether and sorbitan monolaurate EO adduct (1 to 30 moles added per active hydrogen) and sorbitan monooleate EO adduct (1 to 30 moles added per active hydrogen) 30) and other polyhydric (2-8 valent or higher) alcohols (2-30 carbon atoms) fatty acids (8-24 carbon atoms) ester EO adducts (1-30 adducts per active hydrogen) And so on.

Examples of the polyhydric alcohol-type nonionic surfactant (A-1-2) include polyvalent (2 to 8-valent or higher) such as glycerin monostearate, glycerin monooleate, sorbitan monolaurate and sorbitan monoolate. Examples thereof include fatty acid (8 to 24 carbon atoms) esters of alcohols (2 to 30 carbon atoms) and fatty acid alkanolamides such as lauric acid monoethanolamide and lauric acid diethanolamide.

As the anionic surfactant (A-2), an alkyl ether carboxylic acid having 8 to 24 carbon atoms or a salt thereof and an alkyl (poly) oxyethylene ether carboxylic acid having 8 to 24 carbon atoms or a salt thereof [(poly) oxy Ethylene (degree of polymerization = 1 to 100) sodium lauryl ether acetate and (poly) oxyethylene (degree of polymerization = 1 to 100) sodium lauryl sulfosuccinate, etc.], alkyl sulfate ester salts having 8 to 24 carbon atoms and 8 to 8 carbon atoms Twenty-four alkyl (poly) oxyethylene sulfate ester salts [sodium lauryl sulfate, lauryl (poly) oxyethylene (polymerization degree = 1 to 100) sodium sulfate and lauryl (poly) oxyethylene (polymerization degree = 1 to 100) sulfate-tri Ethanolamine salt, etc.], palm oil fatty acid monoethanolamide sodium sulfate sulfonate, alkylphenyl sulfonate with 8 to 24 carbon atoms [sodium dodecylbenzene sulfonate, etc.], alkylphosphate ester salt with 8 to 24 carbon atoms and carbon Alkyl (poly) oxyethylene phosphate ester salts of number 8 to 24 [sodium lauryl phosphate and (poly) oxyethylene (polymerization degree = 1 to 100) sodium lauryl ether phosphate, etc.], fatty acid salts [sodium lauryl sulfate and laurin Acid triethanolamine, etc.], acylated amino acid salt [sodium coconut oil fatty acid methyl taurine, coconut oil fatty acid sarcosin sodium, coconut oil fatty acid sarcosin triethanolamine, N-palm oil fatty acid acyl-L-glutamate triethanolamine, N -Palm oil fatty acid acyl-L-sodium glutamate, sodium lauroylmethyl-β-alanine, etc.].

Examples of the cationic surfactant (A-3) include quaternary ammonium salt type [stearyltrimethylammonium chloride, behenyltrimethylammonium chloride, distearyldimethylammonium chloride and lanolin fatty acid ethyl sulfate aminopropylethyldimethylammonium, etc.] and amine salts. Types [diethylaminoethylamide stearate, dilaurylamine hydrochloride, oleylamine, etc.] and the like can be mentioned.

Examples of the amphoteric tenside agent (A-4) include betaine-type amphoteric tenside agents [coconut oil fatty acid amidopropyldimethylaminoacetic acid betaine, lauryldimethylaminoacetic acid betaine, 2-alkyl-N-carboxymethyl-N-hydroxyethyl imidazole. Nium betaine, lauryl hydroxysulfobetaine, lauroylamide ethyl hydroxyethyl carboxymethyl betaine sodium hydroxypropyl phosphate, etc.], amino acid amphoteric tenside [β-lauryl aminopropionate, etc.] can be mentioned.

Examples of biosurfactant (A-5) include surfactants, ramnolipids, and salts thereof. Examples of the salt include alkali metal salts, alkaline earth metal salts, onium salts and the like.

As the surfactant (A), one type or two or more types can be used. When two or more kinds are used, the combination includes, for example, a nonionic surfactant (A-1) and an anionic surfactant (A-2), a nonionic surfactant (A-1) and a cation. Examples thereof include a combination of an amphoteric surfactant (A-3), a nonionic surfactant (A-1) and an amphoteric surfactant (A-4).

As the surfactant (A), from the viewpoint of detergency, the nonionic surfactant (A-1) can be used alone, and the nonionic surfactant (A-1) and the anionic surfactant (A-1) can be used alone. It is preferable to use it in combination with -2).

As the nonionic surfactant (A-1), an aliphatic alcohol (8 to 24 carbon atoms) ethylene oxide adduct (polymerization degree = 1 to 100) is preferable, and more preferably an aliphatic alcohol, from the viewpoint of detergency. Alcohol (12-18 carbon atoms) ethylene oxide adduct (polymerization degree 4-20), then more preferably aliphatic alcohol (12-15 carbon atoms) ethylene oxide adduct (polymerization degree = 8-12), particularly preferably. Is an 11 mol adduct of lauryl alcohol ethylene oxide.

The anionic surfactant (A-2) includes an alkylphenyl sulfonate having 8 to 24 carbon atoms, a fatty acid salt, an alkyl sulfate ester salt having 8 to 24 carbon atoms, and an alkyl sulfate ester salt having 8 to 24 carbon atoms from the viewpoint of detergency. Alkyl (poly) oxyethylene sulfate ester salt of, more preferably an alkylphenyl sulfonate having 12 to 16 carbon atoms and a fatty acid salt having 8 to 16 carbon atoms, and further preferably dodecylbenzenesulfonic acid monoethanol. Amine salt and sodium laurate.

From the viewpoint of detergency, the content (mass%) of the surfactant (A) contained in the detergent of the present disclosure is preferably 0% by mass or more and 10% by mass or less, more preferably, with respect to the mass of the detergent. Is 0.1% by mass or more and 5% by mass or less.

Enzyme (B) includes protease (B-1), amylase (B-2), lipase (B-3) and cellulase (B-4), aminopeptidase and the like.

In the present disclosure, the protease (B-1) is an enzyme that catalyzes hydrolysis using a peptide or protein as a substrate. The protease (B-1) includes those of animal, plant or microbial origin, and those of microbial origin are preferable from the viewpoint of availability. Also included are chemically or genetically modified variants. The protease (B-1) may be a protease (alkaline protease) having an optimum pH on the neutral to alkaline side, and a plurality of proteases satisfying this condition can be used in combination. Examples of the protease (B-1) include serine protease (B-1-1), aspartic protease (B-1-2), cysteine protease (B-1--3) and metalloprotease (B-1--4). included.

Serine proteases (B-1-1) are proteases that have a serine residue as a catalytic residue, such as chymotrypsin, trypsin, thrombin, plasmin, elastase, subtilisin (subtilisin E, subtilisin BPN'), kexin, and actinomycetes (subtilisin E, subtilisin BPN'). It includes proteases derived from (streptomyces), proteases derived from subtilisin (Bacillus), proteases derived from filamentous fungi (Aspergillus), and the like. Specific examples thereof include trypsin derived from porcine pancreas, subtilisin Novo derived from Bacillus, subtilisin Carlsberg, subtilisin 309, subtilisin 147 and subtilisin 168. Serine protease (B-1-1) is a protease in which a serine residue is involved in the active center, and is inactivated by a drug such as diisopropylfluorophosphate or phenylmethanesulfonylfloride that specifically binds to the serine residue. It has been known. Serine protease (B-1-1) does not require a reducing agent, is not affected by metal chelating agents, and has an optimum pH of enzyme activity near neutrality, and is therefore preferably used in the present disclosure. ..

Commercially available serine proteases (B-1-1) include Alcalase, Sabinase, Evalase, Esperase, Cannase, Ovozyme, Subtilisin A, PEM, PTN, Primase, Durazym manufactured by Novozymes, and Bioplase manufactured by Nagase Biochemical Industry Co., Ltd. , Protease N "Amano" manufactured by Amano Pharmaceutical Co., Ltd., Protease P "Amano", Actinase AS manufactured by Kaken Pharmaceutical Co., Ltd., KAP manufactured by Kao Co., Ltd. Examples thereof include pronase, TrypLE Select manufactured by Invitrogen Co., Ltd., and Accutase manufactured by Chemicon International. Further, the protease described in JP-A-2007-61101 can also be preferably used.

Aspartic protease (B-1-2) is a protease in which aspartic acid is present in the active center, and includes pepsin, cathepsin D, cathepsin E, renin, chymosin, and the like. Specific examples include pepsin derived from the human stomach. Aspartic protease (B-1-2) is a protease generally also called an acidic protease and has enzymatic activity in the acidic region. HFIP is suitable because it is acidic.

Cysteine protease (B-1--3) is a protease in which a thiol group is present in the active center, and includes papain, bromelain, ficin, actinidin, cathepsin B, cathepsin H, cathepsin L, caspase, ginger protease and the like. Since the active center of cysteine protease (B-1--3) is a thiol group, it is preferable to use a reducing agent such as cysteine or thiourea in combination. From the viewpoint of preventing oxidation by oxygen in the air, such a reducing agent is preferably added to the cleaning agent immediately before or during cleaning.

The metalloprotease (B-1--4) is a protease containing a metal ion in the active center, and examples thereof include thermolysin, matrix metalloproteinase, carboxypeptidase A, carboxypeptidase B, dispase, and collagenase. Examples of commercially available metalloproteases (B-1-4) include dispase manufactured by Worthington Biochemical Corporation. In one embodiment, when the metalloproteinase (B-1-4) and the chelating agent (C) described later are used in combination, it is preferable to use a metal-free chelating agent from the viewpoint of maintaining the activity of the metalloproteinase.

Among the above proteases (B-1), serine protease (B-1-1) and aspartic protease (B-1-2) are preferable, and subtilisin and plasmin are more preferable, from the viewpoint of long-lasting effect and detergency. Is. Of these, subtilisins derived from Bacillus Hallodurans and Bacillus clausii are preferable.

By containing the protease (B-1), the cleaning agent of the present disclosure can more efficiently clean the adhered protein stains. The protease (B-1) may be contained in the cleaning agent, but the cleaning agent containing the protease (B-1) may be used in combination with the cleaning agent of the present disclosure. From the viewpoint of enzyme stability, it is preferable to separately prepare a cleaning agent containing protease (B-1) and use it in combination immediately before or during washing.

Amylase (B-2) includes those of bacterial or fungal origin. Also included are chemically or genetically modified variants. Examples of amylase (B-2) are described in detail in British Patent No. 1,296,839. Examples thereof include α-amylase obtained from a special strain of B. licheniformis. Examples of commercially available amylase (B-2) include Duramyl, Termamyl, Fungamyl and BAN manufactured by Novozymes, and Rapidase and Maxamyl P manufactured by Gist-Brocades.

Lipase (B-3) includes those of bacterial or fungal origin. Also included are chemically or genetically modified variants. Examples of lipases include Humicola langinosa lipase (European Patent No. 258 068 and European Patent No. 30 216), Rhizomucor miehei lipase and Candida (Candida). Patent No. 238, 023), C.I. C. ntarctica lipases A and B, Pseudomonas lipases (European Patent No. 214, 761), P. et al. P. pseudoalcaligenes and P. pseudoalcaligenes. Alcaligenes lipase (European Patent No. 218, 272), P. et al. P. cepasia lipase (European Patent No. 331, 376), P. et al. P. stutzeri lipase, P. P. Fluorescence lipase and Bacillus lipase (UK Pat. No. 1,372,034), B.I. B. subtilis lipase (Dartois et al. (1993), Biochemica et Biophysica Acta1131,253-260), B.I. B. stearothermophilus lipase (Japanese Patent Publication No. 64-744922) and B. B. pumilus lipase (International Publication No. 91/16422) can be mentioned.

Examples of commercially available lipase (B-3) include M1 Lipase, Luma fast and Lipomax from Genecore, Lipase and Lipase Ultra from Novozymes, and Lipase P "Amano" from Amano Enzyme.

Cellulase (B-4) includes those of bacterial or fungal origin. Also included are chemically or genetically modified variants. Cellulases include those disclosed in US Pat. No. 4,435,307 as fungal cellulases produced from Humicola insolence.

Examples of commercially available cellulases include Cellulase of Novozymes Co., Ltd. and KAC-500 (B) of Kao Corporation produced by the strain of Humicola insolence.

Of the above enzymes (B), protease (B-1) is preferable from the viewpoint of detergency.

In the present disclosure, the enzyme (B) contained in the cleaning agent may contain two or more types. Examples of the combination containing two or more kinds include a combination containing two or more kinds of protease, protease and amylase, protease and lipase, or protease and amylase and lipase.

The content (% by mass) of the enzyme (B) contained in the detergent of the present disclosure is preferably 0% by mass or more and 10% by mass or less, more preferably 0, with respect to the mass of the cleaning agent from the viewpoint of detergency. It is 0.05% by mass or more and 5% by mass or less, and particularly preferably 0.1% by mass or more and 3% by mass or less.

As the chelating agent (C), any of aminocarboxylic acid type, organic acid type, phosphonic acid type, phosphoric acid type and polycarboxylic acid type can be used. For example, aminopolyacetic acids such as nitrilotriacetic acid, iminodiacetic acid, ethylenediaminetetraacetic acid (EDTA), diethylenetriaminepentaacetic acid, glycol etherdiaminetetraacetic acid, hydroxyethyliminodiacetic acid, triethylenetetraaminehexacetic acid, diencoric acid, etc. Organic acids such as salts, diglycolic acid, oxydisuccinic acid, carboxymethyloxysuccinic acid, citric acid, lactic acid, tartrate acid, oxalic acid, malic acid, gluconic acid, carboxymethyl succinic acid, carboxymethyl tartrate acid, glutamate diacetic acid, etc. Salt, aminotri (methylenephosphonic acid), 1-hydroxyethylidene-1,1-diphosphonic acid, ethylenediaminetetra (methylenephosphonic acid), diethylenetriaminepenta (methylenephosphonic acid) and other phosphonic acids or salts thereof, tripolyphosphate and other phosphorus Examples thereof include an acid or a salt thereof, a polycarboxylic acid such as polyacrylic acid, polymethacrylic acid and polymaleic acid, or a salt thereof. From the viewpoint of versatility, the chelating agent (C) is preferably one or more selected from aminopolyacetic acid and a salt thereof, and more preferably one or two selected from ethylenediaminetetraacetic acid (EDTA) and a salt thereof. That is all. Examples of the counterion of these salts include alkali metals, quaternary amines, alkanolamines, etc., but alkanolamine salts are preferable from the viewpoint of corrosion resistance to medical instruments. Further, a monoethanolamine salt is preferable. These can be used alone or in combination of two or more.

The content (mass%) of the chelating agent (C) contained in the cleaning agent of the present disclosure is 0% by mass or more and 5% by mass or less with respect to the mass of the cleaning agent from the viewpoint of the effect of removing protein stains and the cost. Yes, 0.005% by mass or more and 2% by mass or less is more preferable, and 0.01% by mass or more and 1% by mass or less is further preferable. As the content of the chelating agent (C), an acid equivalent amount is used.

As the enzyme stabilizer (D), a monosaccharide, a polysaccharide or a boron compound can be used. The monosaccharide and polysaccharide may be substituted or unsubstituted, and may be branched or linear. Examples of monosaccharides and polysaccharides include dextrin, glucose, mannose and the like. The boron compound may be substituted or unsubstituted. Examples of the boron compound include boric acid, diboron trioxide, boronic acid, and salts thereof. The content (mass%) of the enzyme stabilizer (D) contained in the detergent of the present disclosure is preferably 0% by mass or more and 10% by mass or less, more preferably 0% by mass or less, based on the mass of the detergent, from the viewpoint of detergency. Is 0.05% by mass or more and 5% by mass or less, and particularly preferably 0.1% by mass or more and 3% by mass or less.

The anticoagulant (E) includes a sugar (E-1), a nonionic surfactant (E-2), an organic acid or a salt thereof (E-3), an inorganic oxo acid or a salt thereof (E-4). , Glycerin (E-5) is included.

The sugar (E-1) includes allose, altrose, mannose, growth, idose, galactose, tarose, monosaccharides having 3 to 5 carbon atoms, ketose having 6 carbon atoms, polysaccharides having disaccharides or more, and 4 carbon atoms. Examples thereof include ~ 12 sugar alcohols. Examples of monosaccharides having 3 to 5 carbon atoms include glyceraldehyde, erythrulose, erythrose, ribose, xylulose, and xylulose. Examples of ketose having 6 carbon atoms include fructose and sorbose. Examples of the disaccharide or higher polysaccharide include sucrose, lactose, trehalose, cellobiose, sophorose, raffinose, maltotriose, carboxylmethylcellulose, starch, pullulan, pectin, glucomannan and the like. Examples of sugar alcohols having 4 to 12 carbon atoms include sorbitol, xylitol, pentaerythritol, maltitol, lactitol, sucralose and the like.

Examples of the non-surface active agent (E-2) include higher alcohol alkylene oxide adduct, alkyl (or alkenyl) phenol alkylene adduct adduct, styrated phenol alkylene oxide adduct, styrated alkyl phenol alkylene oxide adduct, and higher alkyl (or higher alkyl (or alkenyl) adduct. Higher alkenyl) amine alkylene oxide adduct, fatty acid alkylene oxide adduct, fatty acid amide alkylene oxide adduct, polypropylene glycol alkylene oxide adduct, (mono or poly) glycerol fatty acid adduct or alkylene oxide adduct thereof, sucrose fatty acid ester or its Examples thereof include an alkylene oxide adduct, a sorbitan fatty acid ester, or an alkylene oxide adduct thereof.

Here, the higher alcohol is usually a linear or branched unsaturated or saturated higher alcohol having 8 to 24 carbon atoms, and the alkyl or alkenylphenol is usually a linear or branched alkyl group or alkenyl group having 6 to 22 carbon atoms. The higher alkyl or higher alkenylamine is usually a linear or branched higher alkyl or higher alkenylamine having 8 to 24 carbon atoms, and the fatty acid is usually an unsaturated or saturated fatty acid having 8 to 24 carbon atoms. The weight average molecular weight of polypropylene glycol is 900 to 5000.

Examples of the alkyleneoxy group of the alkylene oxide adduct include ethyleneoxy group, propyleneoxy group, butyleneoxy group, and styreneoxy group, which may be used alone or in combination of two or more. When two or more kinds are used, the addition form of the alkylene oxide is not limited, and examples thereof include random addition, block addition, and a method of mixing random and block. The number of moles of the alkylene oxide adduct added is 1 to 1000.

Examples of the organic acid or a salt thereof (E-3) include amino acids, aminocarboxylic acids, keto acids, oxycarboxylic acids, polycarboxylic acids, saturated or unsaturated fatty acids having 1 to 24 carbon atoms, and salts thereof. .. Examples of the organic acid include organic compounds having an acidic group such as a carboxy group, a sulfo group, a phosphoric acid group, a thiol group, and a phenolic hydroxyl group in the molecule, and examples of these salts include alkali metal salts. Examples thereof include ammonium salts and alkanolamine salts.

More specifically, examples of the organic compound having a carboxy group include amino acids, aminocarboxylic acids, keto acids, oxycarboxylic acids, polycarboxylic acids, saturated or unsaturated fatty acids having 1 to 24 carbon atoms, and the like. Examples of the organic compound having a sulfo group include benzenesulfonic acid, linear alkylbenzenesulfonic acid, α-olefin sulfonic acid, sulfuric acid monoester and the like. Examples of the organic compound having a phosphoric acid group include adenylic acid, ethidroic acid, phosphinic acid monoester salt, phosphoric acid monoester, and phosphoric acid diester. Examples of the organic compound having a thiol group include 4-mercaptoacetophenone, thiosalicylic acid, thiobenzoic acid, thioglycolic acid and the like. Examples of the organic compound having a phenolic hydroxyl group include phenol, 2-naphthol, catechol and the like. Examples of the alkali metal salt include sodium salt and potassium salt. Examples of the alkanolamine salt include monoethanolamine salt, diethanolamine salt, triethanolamine salt and the like.

Examples of the inorganic oxoacid or a salt thereof (E-4) include phosphates, hypophosphates, pyrophosphates, polyphosphates, sulfates and the like. Examples of the inorganic oxo acid are oxo acids such as phosphorus, sulfur, nitrogen, boron, chlorine, bromine, iodine and silicon, and examples of these salts include alkali metal salts, ammonium salts and alkanolamine salts.

More specifically, examples of phosphorus oxoacids include hypophosphorous acid, phosphoric acid, phosphoric acid, pyrophosphoric acid, and polyphosphates having a degree of polymerization of 3 to 6. Examples of sulfur oxoacids include hyposulfurous acid, sulfite, sulfuric acid, persulfurous acid, pyrosulfuric acid, disulfurous acid, thiosulfuric acid, sulfamic acid, amidosulfuric acid, and dithionous acid. Examples of the oxo acid of nitrogen include nitrite and nitric acid. Examples of the oxo acid of boron include metaboric acid, boric acid, and perboric acid. Examples of the oxo acid of chlorine include hypochlorous acid, chloric acid, chloric acid, perchloric acid and the like. Examples of the bromic acid of bromine include hypobromous acid, bromous acid, bromic acid, and perbromic acid. Examples of the oxo acid of iodine include hypoiodous acid, iodic acid, and periodic acid. Examples of the oxo acid of silicon include ortho-silicic acid, meta-silicic acid, meta-silicic acid and the like. Examples of the alkali metal salt include sodium salt, potassium salt and the like, and examples of the alkanolamine salt include monoethanolamine salt, diethanolamine salt, triethanolamine salt and the like.

The cleaning agent of the present disclosure can clean blood stains more efficiently by containing the anticoagulant (E). For example, the content (mass%) of glycerin (E-5) contained in the detergent of the present disclosure is 0% by mass with respect to the mass of the detergent from the viewpoint of the blood coagulation preventing effect and the coagulating blood dissolving effect. It is 80% by mass or less.

A silicate can be used as the metal corrosion inhibitor (F). For example, examples of the silicate include alkali metal silicates. As the alkali metal silicate, a compound in which n of _{M 2} O · nSiO _{2 is 0.3 to 5 is used.} Further, a more preferable value of n is 1 to 3. From the viewpoint of light metal corrosion suppression performance, n is preferably 0.3 or more, and from the viewpoint of preventing scale generation derived from silicic acid, n is preferably 5 or less. Examples of the alkali metal silicate include potassium orthosilicate, sodium orthosilicate, sodium sesquisilicate, potassium sesquisilicate, sodium metasilicate, potassium metasilicate, and sodium No. 1 sodium silicate and sodium No. 2 silicate specified in JIS K1408. No. 3 sodium silicate, trade name manufactured by Nippon Kagaku Kogyo Co., Ltd.: 1K potassium silicate, 2K potassium silicate, A potassium silicate (pure content 40%) (molar ratio K ₂ O: SiO ₂ = 1: 3), etc. Be done. The content (mass%) of the metal corrosion inhibitor (F) contained in the cleaning agent of the present disclosure shall be 0% by mass or more and 10% by mass or less with respect to the mass of the cleaning agent from the viewpoint of corrosion suppression performance. Is preferable.

The low molecular weight polyol (G) contains an alcohol compound having at least two hydroxyl groups and having 2 to 30 carbon atoms.

Examples of the low molecular weight polyol (G) include ethylene glycol, diethylene glycol, triethylene glycol, tetraethylene glycol, propylene glycol, dipropylene glycol, tripropylene glycol, propanediol, butanediol, methylpropanediol, pentanediol, and methylbutane. Diol, ethylpropanediol, dimethylpropanediol, hexanediol, methylpentanediol, ethylmethylpropanediol, dimethylbutanediol, heptanediol, propylmethylpropanediol, isopropylmethylpropanediol, octanediol, ethylhexanediol, sec-butylbutyl Propanediol, dimethylhexanediol, trimethylpentanediol, nonanediol, octanediol, ethyl (2-methyl) propylpropanediol, trimethylhexanediol, butylethylpropanediol, diethylpentanediol, methyloctanediol, decanediol, dimethyloctanediol , Undecanediol, ethylmethyloctanediol, dodecanediol, diethyloctanediol, trimethylnonanediol, tetradecanediol, pentadecanediol, hexadecanediol, heptadecanediol, octadecanediol, eicosandiol, docosandiol, tetracosanediol, etc. Examples thereof include saturated diols and condensates thereof.

In addition, butenediol, methylenepropanediol, butinediol, hexenediol, methylpentenediol, hexadienediol, octenediol, dimethylhexenediol, decenediol, dimethyloctenediol, tetradecenediol, hydroxyoctadesenol, pentynediol, Hexindiol, methylpentindiol, heptindiol, dimethylpentindiol, dimethylhexindiol, decinediol, dimethyloctinediol, tetramethyloctinediol, tetramethyldecinediol, tetramethyldodecinediol, tetraisopropyloctinediol , Unsaturated diols such as diethyltetradecine diol and the like.

In addition, aliphatic cyclic diols such as cyclopentanediol, cyclohexanediol, cycloheptandiol, norbornandiol, cyclooctanediol, cyclodecanediol, cyclooctenediol, decalindiol, limonene glycol, terpendiol, bicyclohexanediol, and cyclododecanediol, etc. Can be mentioned.

In addition, glycerin, butanetriol, methylpropanetriol, pentantriol, methylbutanetriol, trimethylolethane, hexanetriol, ethylbutanetriol, trimethylolpropane, propylheptantriol, dimethylpentanetriol, triethanolamine, triisopropanolamine, etc. Triol alcohol and the like can be mentioned.

In addition, erythritol, pentaerythritol, pentatetrol, hexatetrol, pentantetrol, hexanetetrol, diglycerin, sorbitan, N, N, N', N'-tetrakis (2-hydroxypropyl) ethylenediamine, N, N , N', N'-tetrakis (hydroxyethyl) ethylenediamine and other tetrahydric alcohols and the like.

Examples thereof include pentahydric alcohols such as adonitol, arabitol, xylitol and triglycerin, and hexahydric alcohols such as dipentaerythritol, sorbitol, mannitol, iditol, inositol, darcitol, tarose and allose.

In addition, methyl glyceryl ether, ethyl glyceryl ether, propyl glyceryl ether, isopropyl glyceryl ether, butyl glyceryl ether, isobutyl glyceryl ether, pentyl glyceryl ether, hexyl glyceryl ether, heptyl glyceryl ether, octyl glyceryl ether, (2-ethylhexyl) glyceryl ether, Nonyl glyceryl ether, decyl glyceryl ether, undecyl glyceryl ether, dodecyl glyceryl ether, tridecyl glyceryl ether, tetradecyl glyceryl ether, hexadecyl glyceryl ether, octadecyl glyceryl ether, eicosyl glyceryl ether, allyl glyceryl ether, undecyl glyceryl ether , Oleyl glyceryl ether, cyclohexyl glyceryl ether, phenyl glyceryl ether and other glycerin monoethers and the like.

Further, N-substituted diethanolamines such as N-methyldiethanolamine, N-ethyldiethanolamine, N-propyldiethanolamine, N-isopropyldiethanolamine, N-butyldiethanolamine, N-cyclohexyldiethanolamine, N- (2-ethylhexyl) diethanolamine and the like can be mentioned. Be done.

In addition, N-methyldiisopropanolamine, N-ethyldiisopropanolamine, N-propyldiisopropanolamine, N-isopropyldiisopropanolamine, N-butyldiisopropanolamine, N-cyclohexyldiisopropanolamine, N- (2-ethylhexyl) ) N-substituted diisopropanolamines such as diisopropanolamine and the like can be mentioned.

Further, N, N-di-substituted aminopropanediols such as dimethylaminopropanediol, diethylaminopropanediol, dipropylaminopropanediol, diisopropylaminopropanediol and dibutylaminopropanediol can be mentioned. These exemplified low molecular weight polyols (G) may also include position isomer compounds.

The content (mass%) of the low molecular weight polyol (G) contained in the cleaning agent of the present disclosure is preferably 0% by mass or more and 80% by mass or less with respect to the mass of the cleaning agent from the viewpoint of detergency.

Cleaning aids (builders) (H) include polycarboxylic acids (acrylic acid homopolymers, maleate homopolymers, etc.), polyvalent carboxylates (citric acid, malic acid, etc.), and alkaline builders (caustic soda). , Soda ash, ammonia, triethanolamine, sodium tripolyphosphate, sodium silicate, etc.) and the like. The content (% by mass) of the builder (H) contained in the cleaning agent of the present disclosure is preferably 0% by mass or more and 20% by mass or less with respect to the mass of the cleaning agent from the viewpoint of detergency.

Examples of the defoaming agent (I) include silicone-based defoaming agents, polyoxyalkylene-based defoaming agents, and mineral oil-based defoaming agents. The content (mass%) of the defoaming agent (I) contained in the cleaning agent of the present disclosure is preferably 0% by mass or more and 10% by mass or less with respect to the mass of the cleaning agent from the viewpoint of detergency.

Examples of the pH adjuster (J) include sulfuric acid, hydrochloric acid, citric acid, lactic acid, pyruvic acid, formic acid, sodium chloride, potassium chloride, monoethanolamine and diethanolamine. The content (mass%) of the pH adjuster (J) contained in the detergent of the present disclosure is preferably 0% by mass or more and 25% by mass or less, more preferably 25% by mass or less, based on the mass of the detergent, from the viewpoint of detergency. Is 0% by mass or more and 15% by mass or less, and particularly preferably 0% by mass or more and 10% by mass or less. In one embodiment, when the pH is on the alkaline side, a salt derived from the cleaning agent remains on the surface of the article to be washed and dried, and a treatment such as wiping may be required to remove the salt. Therefore, depending on the object to be cleaned, the cleaning agent of the present disclosure may be preferably acidic, neutral, or weakly alkaline, and may be particularly preferable to be acidic or neutral. Specifically, the pH (25 ° C.) of the cleaning agent of the present disclosure may be preferably less than 9.0, and particularly preferably 7.0 or less. The above does not prevent the cleaning agent of the present disclosure from being used outside this range. Here, the above pH (25 ° C.) is a value measured by a method based on JIS Z8802: 2011 “pH measurement method”.

The concentration of the fluoroalcohol when acting on the virus may be 0.1% by mass or more, preferably 1% by mass or more, and more preferably 8% by mass or more. The concentration of the fluoroalcohol when acting on the virus is more preferably 29% by mass or more. The time for the fluoroalcohol to act on the virus is not particularly limited. The time for the fluoroalcohol to act on the virus is effective even for a short time. For example, the time for allowing the fluoroalcohol to act on the virus may be 30 seconds or longer, preferably 1 minute or longer, more preferably 20 minutes or longer, and particularly preferably 30 minutes or longer.

In one embodiment, the cleaning agent may contain the active ingredient of a commercially available disinfectant as an additive. Examples of the active ingredient of a commercially available disinfectant include an active ingredient of a high-level disinfectant (for example, glutarualdehyde, orthophthalaldehyde, peracetic acid or peracetate), and an active ingredient of a medium-level disinfectant (hypochlorite). Sodium, ethanol, povidone iodine), active ingredients of low-level disinfectants (quaternary ammonium, chlorhexidine gluconate). The content (mass%) of these active ingredients contained in the cleaning agent of the present disclosure may be 0% by mass or more and 99.9% by mass or less, and 0% by mass or more and 99 by mass with respect to the mass of the cleaning agent. It is preferably 0% by mass or more, particularly preferably 0% by mass or more and 92% by mass or less, and further preferably 0% by mass or more and 71% by mass or less.

Fluorine-based alcohols contained in the cleaning agents of the present disclosure, especially HFIP, are effective in inactivating viruses. In addition, HFIP can lyse organic substances such as proteins and cell tissues. Therefore, it becomes easy to remove organic substances such as proteins and cell tissues adhering to the object to be cleaned, and the cleaning power can be improved. HFIP is a stable low molecular weight compound having a molecular weight of 168 and has high storage stability. Further, since it has good thermal stability, the cleaning temperature is not limited, and the cleaning power can be further improved. HFIP is less corrosive to metals and has less effect on the material of the object to be cleaned. Furthermore, HFIP is nonflammable and easy to manage safety in use.

In addition, TFIPL is effective in inactivating the virus. In addition, TFIPL can lyse organic substances such as proteins and cell tissues. Therefore, it becomes easy to remove organic substances such as proteins and cell tissues adhering to the object to be cleaned, and the cleaning power can be improved. TFIPL is a stable low molecular weight compound having a molecular weight of 114.07 and has high storage stability. Further, since it has good thermal stability, the cleaning temperature is not limited, and the cleaning power can be further improved. TFIPL has low corrosiveness to metals and has little effect on the material of the object to be cleaned. Furthermore, TFIPL is nonflammable and safety management in use is easy.

[Washing method]
The cleaning agents containing fluoroalcohol of the present disclosure can be used particularly for cleaning medical devices (including, for example, endoscopes), and further include animal medical devices, meat processing tools, and cooking tools. It can be used for cleaning things. However, the present invention is not limited to this, and can be widely applied to virus infection control. For example, it can be used for cleaning an operating room in the medical field, linen such as beds and sheets, and cleaning an object including disinfection of human hands.

The cleaning agents containing fluoroalcohol of the present disclosure include, for example, manual cleaning (including immersion cleaning), ultrasonic cleaning, jet cleaning, shower cleaning, steam cleaning, vacuum cleaning, degassing cleaning, washer-disinfector, or It can be used in any of these two or more combinations of cleaning methods, but is not limited thereto. For example, a cleaning method using a washer-disinfector generally consists of steps of pre-cleaning, main cleaning, rinsing, and disinfection. The cleaning agent containing a fluorinated alcohol of the present disclosure can be used for pre-cleaning and main cleaning, and can also be used in combination with other cleaning agents for pre-cleaning or main cleaning. Here, the concentration of the fluorine-based alcohol contained in the acceptable detergent is the same as the concentration that inactivates the virus, and specifically, it may be 0.1% by mass or more, and 1% by mass or more. Is preferable, 8% by mass or more is more preferable, and 29% by mass or more is most preferable.

By using the cleaning agent containing a microbial alcohol of the present disclosure in the pre-cleaning step and / or the main cleaning step of the cleaning method, blood, body fluid, fat, and prion protein (Prion Protein, PrP) derived from a living body can be efficiently derived from an object. ) And infectious amyloids, organic substances such as cell tissues, microorganisms, viruses, bacteria and the like can be removed.

However, the cleaning agent containing a fluoroalcohol of the present disclosure is not limited to the above-mentioned uses, and may be used in the same manner as known antiviral agents, antibacterial agents, bactericidal agents, disinfectants, fungicides, etc. depending on the object to be cleaned. can do. For example, a method of spraying on an object to be cleaned, a method of applying, a method of impregnating the object, a method of immersing the object, a method of exposing the object to high-pressure steam, and the like, which are usually adopted, can be used as they are. ..

The temperature at which the fluorinated alcohol contained in the cleaning agent of the present disclosure inactivates the virus is not particularly limited, and a temperature of room temperature or higher (for example, 20 ° C or higher) is preferable. The higher the temperature at which the fluoroalcohol is brought into contact with the virus, the easier it is for the virus to be inactivated. Further, the temperature may be higher than the boiling point of the fluoroalcohol, that is, vaporized into vapor and brought into contact with the virus. In addition, the optimum temperature can be selected depending on the solvent and additives contained in the cleaning agent.

[Washing device]
In one embodiment, it is possible to provide a cleaning device including the action of the above-mentioned fluorinated alcohol according to the present disclosure.

For example, the cleaning device 1 may be used for cleaning medical instruments. As shown in FIG. 1, the cleaning device 1 includes a cleaning tank 10 having a storage unit 20 for accommodating an object (medical instrument), and a cleaning agent supply device 40 for supplying a cleaning agent into the cleaning tank 10. Be prepared. The washing tank 10 has a water storage unit 12 that stores wash water under the storage unit 20, and a water supply source and water supply as a washing water supply means for supplying water and hot water sent from the hot water supply source to the water storage unit 12 as wash water. The pipe 14, the hot water supply pipe 16, the cleaning nozzle 22 that injects the cleaning water of the water storage unit 12 onto the object housed in the cleaning tank 10, the cleaning pump 18 that sends the cleaning water of the water storage unit 12 to the cleaning nozzle 22. To be equipped with.

In the cleaning method using the cleaning device 1, after cleaning the medical equipment by the preliminary cleaning step, the main cleaning step and the rinsing cleaning step, the medical equipment is disinfected with boiling water by the disinfection step. The cleaning agent containing a fluorinated alcohol of the present disclosure can be used for pre-cleaning and main cleaning, and can also be used in combination with other cleaning agents for pre-cleaning or main cleaning. Further, when the cleaning agent containing a fluoroalcohol of the present disclosure contains a plurality of components, some of the components or each component can be used for pre-cleaning or main cleaning from separate lines.

When the cleaning process is started, the cleaning device 1 first executes the preliminary cleaning process. The cleaning device 1 opens the water supply valve and starts the water supply treatment in the preliminary cleaning process. As a result, the water from the water supply source is sent out to the water storage unit 12 of the washing tank 10 through the water supply pipe 14. The cleaning device 1 determines whether or not the water in the water storage unit 12 has reached a predetermined water level by detecting the float switch. When the cleaning device 1 determines that the water in the water storage unit 12 has reached a predetermined water level, the cleaning device 1 closes the water supply valve and ends the water supply process. The water in the pre-cleaning step may be at room temperature (eg 20 ° C.).

As a preliminary cleaning process, the cleaning device 1 supplies the cleaning agent into the cleaning tank 10 by the cleaning agent supply device 40, and operates the cleaning pump 18 for a predetermined time. As a result, the cleaning water in the water storage unit 12 is sent to the cleaning nozzle 22 via the circulation pipe, and the cleaning nozzle 22 injects the cleaning water onto the medical equipment while rotating to perform cleaning. The cleaning water containing the cleaning agent in the cleaning tank 10 falls and returns to the water storage unit 12. After the pre-cleaning treatment, the cleaning device 1 operates the drainage pump for a predetermined time as a wastewater treatment. As a result, the washing water in the water storage unit 12 is discharged to the outside of the machine through the drain pipe 34.

When the pre-cleaning process is completed, the cleaning device 1 executes the main cleaning process. In the main cleaning step, the cleaning device 1 opens the water supply valve and the hot water supply valve to start the water supply process in the main cleaning step. As a result, the water from the water supply source and the hot water from the hot water supply source are sent out to the water storage unit 12 of the washing tank 10 through the water supply pipe 14 and the hot water supply pipe 16. The cleaning device 1 determines whether or not the water in the water storage unit 12 has reached a predetermined water level by detecting the float switch. When the cleaning device 1 determines that the water in the water storage unit 12 has reached a predetermined water level, the cleaning device 1 closes the water supply valve and the hot water supply valve to end the water supply process. The water in this washing step is preferably 25 ° C. or higher.

As the main cleaning process, the cleaning device 1 supplies the cleaning agent into the cleaning tank 10 by the cleaning agent supply device 40, and operates the cleaning pump 18 for a predetermined time. As a result, the cleaning water in the water storage unit 12 is sent to the cleaning nozzle 22 via the circulation pipe, and the cleaning nozzle 22 injects the cleaning water onto the medical equipment while rotating to perform cleaning. The cleaning water containing the cleaning agent in the cleaning tank 10 falls and returns to the water storage unit 12. After the main cleaning treatment, the cleaning device 1 operates the drainage pump for a predetermined time as the wastewater treatment. As a result, the washing water in the water storage unit 12 is discharged to the outside of the machine through the drain pipe 34.

This cleaning step may be executed multiple times. In this case, for example, the main cleaning treatment may be performed a plurality of times by using different cleaning agents. When the cleaning agent containing the fluorinated alcohol of the present disclosure is used in the first main cleaning step, the main cleaning treatment may be performed with cleaning water at 60 ° C. or higher. When an enzyme-based cleaning agent is used in the second main cleaning step, the main cleaning treatment may be performed with cleaning water at about 30 to 40 ° C. (for example, 37 ° C.).

When the main cleaning process is completed, the cleaning device 1 executes the rinsing process. In the rinsing washing step, the washing device 1 opens the hot water supply valve and starts the hot water supply process in the rinsing step. As a result, the hot water of the hot water supply source is sent to the water storage unit 12 of the washing tank 10 through the hot water supply pipe 16. The cleaning device 1 determines whether or not the hot water in the water storage unit 12 has reached a predetermined water level by detecting the float switch. When the cleaning device 1 determines that the hot water in the water storage unit 12 has reached a predetermined water level, the cleaning device 1 closes the hot water supply valve and ends the hot water supply process.

The cleaning device 1 operates the cleaning pump 18 for a predetermined time as a rinsing process. As a result, the hot water in the water storage unit 12 is sent to the cleaning nozzle 22 via the circulation pipe, and the cleaning nozzle 22 injects hot water onto the medical equipment while rotating to wash away the cleaning water containing the cleaning agent. After the rinsing treatment, the cleaning device 1 operates the drainage pump for a predetermined time as a wastewater treatment. As a result, the rinse water in the water storage unit 12 is discharged to the outside of the machine through the drain pipe 34.

When the rinsing process is completed, the cleaning device 1 executes the disinfection process. In the disinfection step, the cleaning device 1 opens the hot water supply valve and starts the hot water supply process in the disinfection step. As a result, the hot water of the hot water supply source is sent to the water storage unit 12 of the washing tank 10 through the hot water supply pipe 16. The cleaning device 1 determines whether or not the hot water in the water storage unit 12 has reached a predetermined water level by detecting the float switch. When the cleaning device 1 determines that the hot water in the water storage unit 12 has reached a predetermined water level, the cleaning device 1 closes the hot water supply valve and ends the hot water supply process. The boiling water in the disinfection step is preferably 60 ° C. or higher.

The cleaning device 1 operates the cleaning pump 18 for a predetermined time as a disinfection process. As a result, the hot water in the water storage unit 12 is sent to the cleaning nozzle 22 via the circulation pipe, and the cleaning nozzle 22 injects the hot water into the medical instrument while rotating. After the disinfection treatment, the cleaning device 1 operates the drainage pump for a predetermined time as the wastewater treatment. As a result, the hot water in the water storage unit 12 is discharged to the outside of the machine through the drain pipe 34.

The cleaning device 1 efficiently supplies blood, body fluid, fat, and prion protein (Prion) derived from a living body from an object by supplying the cleaning agent containing the microbial alcohol of the present disclosure into the cleaning tank 10 by the cleaning agent supply device 40. Proteins such as Protein, PrP) and infectious amyloids, organic substances such as cell tissues, microorganisms, viruses, bacteria and the like can be removed. The cleaning device 1 can also use a cleaning agent containing a fluorinated alcohol of the present disclosure and a known cleaning agent in combination.

[Soil cleaner]
The cleaning agent according to the present invention described above can be used as a soil cleaning agent in one embodiment. By using the soil cleaning agent containing the fluoroalcohol of the present disclosure, inactivation of the virus contained in the soil can be expected. In one embodiment, the soil cleaner can also include common additives described as known techniques. These also include pesticides, fertilizers, fungicides, disinfectants, etc. used in soil.

In one embodiment, the soil cleaner may contain the fluorinated alcohol of the present disclosure and a solvent. The solvent can be selected from the substances capable of diluting the fluoroalcohol, and examples thereof include the same solvents as those mentioned in the above-mentioned cleaning agents. These may be one or more, but are not limited thereto.

In one embodiment, the content (% by mass) of the solvent contained in the soil cleaning agent may be 0% by mass or more and 99.9% by mass or less with respect to the mass of the soil cleaning agent from the viewpoint of cleaning property. Often, 0% by mass or more and 99% by mass or less is preferable, 0% by mass or more and 92% by mass or less is particularly preferable, and 0% by mass or more and 71% by mass or less is further preferable.

In one embodiment, the soil cleaning agent may contain additives in addition to the fluorinated alcohol and solvent of the present disclosure. Additives that can be added to soil cleaners include surfactants, enzymes, enzyme stabilizers, metal corrosion inhibitors, low molecular weight polyols, cleaning aids (builders), defoamers, pH regulators, fragrances, and colorants. , Antioxidants, preservatives, bleaching agents, bleaching activators, corrosion inhibitors, dispersants, thickeners, viscosity regulators, etc., but are not limited thereto. The soil cleaner may contain one or more additives. In one embodiment, the soil cleaning agent can further improve the cleaning power by containing a fluorine-based alcohol, a solvent, and the above-mentioned additives.

Examples of the surfactant (A) include the same ones as the surfactant (A) mentioned in the above-mentioned cleaning agent. As the surfactant (A), one kind or two or more kinds can be used.

The content (mass%) of the surfactant (A) contained in the soil detergent is preferably 0% by mass or more and 10% by mass or less, more preferably 0% by mass or less, based on the mass of the soil detergent from the viewpoint of cleanability. It is 0.1% by mass or more and 5% by mass or less.

Examples of the enzyme (B) include those similar to the enzyme (B) mentioned in the above-mentioned cleaning agent. As the enzyme (B), one type or two or more types can be used.

In one embodiment, the content (mass%) of the enzyme (B) contained in the soil cleaning agent is preferably 0% by mass or more and 10% by mass or less with respect to the mass of the soil cleaning agent from the viewpoint of cleanability. More preferably, it is 0.05% by mass or more and 5% by mass or less, and particularly preferably 0.1% by mass or more and 3% by mass or less.

Examples of the enzyme stabilizer (C) include those similar to the enzyme stabilizer (D) mentioned in the above-mentioned cleaning agent. As the enzyme stabilizer (C), one kind or two or more kinds can be used. In one embodiment, the content (mass%) of the enzyme stabilizer (C) contained in the soil detergent is 0% by mass or more and 10% by mass or less with respect to the mass of the soil detergent from the viewpoint of detergency. It is preferable, more preferably 0.05% by mass or more and 5% by mass or less, and particularly preferably 0.1% by mass or more and 3% by mass or less.

Examples of the metal corrosion inhibitor (D) include the same as the metal corrosion inhibitor (F) mentioned in the above-mentioned cleaning agent. As the metal corrosion inhibitor (D), one type or two or more types can be used. In one embodiment, the content (mass%) of the metal corrosion inhibitor (D) contained in the soil cleaning agent is 0% by mass or more and 10% by mass with respect to the mass of the soil cleaning agent from the viewpoint of corrosion suppression performance. The following is preferable.

Examples of the low molecular weight polyol (E) include those similar to the low molecular weight polyol (G) mentioned in the above-mentioned cleaning agent. As the low molecular weight polyol (E), one kind or two or more kinds can be used.

In one embodiment, the content (mass%) of the low molecular weight polyol (E) contained in the soil detergent is 0% by mass or more and 80% by mass or less with respect to the mass of the soil detergent from the viewpoint of detergency. preferable.

Examples of the cleaning aid (builder) (F) include the same cleaning aid (builder) (H) mentioned in the above-mentioned cleaning agent. As the builder (F), one type or two or more types can be used. In one embodiment, the content (% by mass) of the builder (F) contained in the soil cleaning agent is preferably 0% by mass or more and 20% by mass or less with respect to the mass of the soil cleaning agent from the viewpoint of detergency.

Examples of the defoaming agent (G) include those similar to the defoaming agent (I) mentioned in the above-mentioned cleaning agent. As the defoaming agent (G), one kind or two or more kinds can be used. In one embodiment, the content (mass%) of the defoaming agent (G) contained in the soil cleaning agent is 0% by mass or more and 10% by mass or less with respect to the mass of the soil cleaning agent from the viewpoint of detergency. preferable.

Examples of the pH adjuster (H) include those similar to the pH adjuster (J) mentioned in the above-mentioned cleaning agent. As the pH adjuster (H), one type or two or more types can be used. In one embodiment, the content (mass%) of the pH adjuster (H) contained in the soil cleaner is 0% by mass or more and 25% by mass or less with respect to the mass of the soil cleaner from the viewpoint of cleanability. It is preferable, more preferably 0% by mass or more and 15% by mass or less, and particularly preferably 0% by mass or more and 10% by mass or less.

The concentration of the fluoroalcohol when acting on the virus may be 0.1% by mass or more, preferably 1% by mass or more, and more preferably 8% by mass or more. The concentration of the fluoroalcohol when acting on the virus is more preferably 29% by mass or more. The time for the fluoroalcohol to act on the virus is not particularly limited. The time for the fluoroalcohol to act on the virus is effective even for a short time. For example, the time for allowing the fluoroalcohol to act on the virus may be 30 seconds or longer, preferably 1 minute or longer, more preferably 20 minutes or longer, and particularly preferably 30 minutes or longer. In order to increase the effect, it is also preferable to use a plurality of times in stages with intervals of use on a daily or weekly basis or even on a seasonal basis.

In one embodiment, the soil cleaning agent may contain the active ingredient of a commercially available disinfectant as an additive. Examples of the active ingredient of the commercially available disinfectant include the same active ingredients of the commercially available disinfectant mentioned in the above-mentioned cleaning agents. In one embodiment, the content (% by mass) of these active ingredients contained in the soil cleaning agent may be 0% by mass or more and 99.9% by mass or less with respect to the mass of the soil cleaning agent, and is 0. It is preferably 0% by mass or more and 99% by mass or less, particularly preferably 0% by mass or more and 92% by mass or less, and further preferably 0% by mass or more and 71% by mass or less.

In one embodiment, fluoroalcohols contained in the soil cleaning agent, especially HFIP and TFIPL, are effective for cleaning soil contaminated with virus. HFIP and TFIPL are stable low molecular weight compounds and have high storage stability. Further, since it has good thermal stability, the cleaning temperature is not limited, and the cleaning power can be further improved. Furthermore, HFIP and TFIPL are nonflammable, and safety management in use is easy.

[Washing method]
The soil cleaning agent according to the present embodiment can be used to clean soil contaminated with a virus or soil that may be contaminated. Alternatively, the soil may be washed prophylactically with the soil cleaning agent according to the present embodiment. In one embodiment, the soil cleaning agent may be diluted with the above solvent and used for cleaning. In one embodiment, the soil cleaning agent or solvent and the soil cleaning agent according to the present embodiment are added to the soil to be cleaned, mixed, and the washed soil is separated and dried to inactivate the virus and soil. Can be washed.

In one embodiment, at the time of washing, the concentration of the fluorinated alcohol or the compound represented by the general formula (1) may be 0.01% by mass or more, preferably 0.1% by mass or more, and 1% by mass or more. Is particularly preferable. It can be adjusted arbitrarily while considering the degree of soil contamination at the time of use, the time of contamination, the surrounding environment of the soil, and the like.

In one embodiment, the fluoroalcohol or the compound represented by the general formula (1) can also be used as a soil fumigant. For example, the soil can be washed by fumigating the soil suspected of being contaminated with a fluorine-based alcohol or a soil fumigant containing a compound represented by the general formula (1).

[Bactericidal cleaning agent]
The above-mentioned cleaning agent according to the present invention can be used as a sterilizing cleaning agent in one embodiment. The fluorinated alcohol of the present disclosure is suitable as a compound for sterilization. In particular, HFIP and TFE are suitable as bactericidal compounds. By using the bactericidal detergent containing the fluoroalcohol of the present disclosure, a bactericidal effect of bacteria can be expected.

In the present specification, the bacteria can be exemplified by Staphylococcus aureus, Escherichia coli, enterohemorrhagic Escherichia coli, tuberculosis, MRSA, vancomycin-resistant enterococci, multidrug-resistant Pseudomonas aeruginosa, multidrug-resistant Acinetobacter, and the like. It is not limited to these.

In one embodiment, the sterilizing detergent may contain the fluorinated alcohol of the present disclosure and a solvent. The solvent can be selected from the substances capable of diluting the fluoroalcohol, and examples thereof include the same solvents as those mentioned in the above-mentioned cleaning agents. These may be one or more, but are not limited thereto.

In one embodiment, the content (% by mass) of the solvent contained in the sterilizing cleaning agent may be 0% by mass or more and 99.9% by mass or less with respect to the mass of the cleaning agent from the viewpoint of detergency. , 0% by mass or more and 99% by mass or less is preferable, 0% by mass or more and 92% by mass or less is particularly preferable, and 0% by mass or more and 71% by mass or less is further preferable.

In one embodiment, the sterilizing detergent may contain additives in addition to the fluorinated alcohol and solvent of the present disclosure. Additives that can be added to sterilizing detergents include surfactants, enzymes, chelating agents, enzyme stabilizers, blood coagulation inhibitors, metal corrosion inhibitors, low molecular weight polyols, cleaning aids (builders), defoaming agents, etc. Acidity regulators, fragrances, colorants, antioxidants, preservatives, bleaching agents, bleach activators, corrosion inhibitors, dispersants, thickeners, viscosity regulators, etc., but are not limited to these. Absent. The sterilizing detergent may contain one or more additives. In one embodiment, the sterilizing detergent can further improve the cleaning power by containing a fluoroalcohol, a solvent, and the above-mentioned additives.

The content (mass%) of the surfactant (A) contained in the sterilizing detergent is preferably 0% by mass or more and 10% by mass or less with respect to the mass of the sterilizing detergent from the viewpoint of detergency, and more preferably. It is 0.1% by mass or more and 5% by mass or less.

In one embodiment, the content (% by mass) of the enzyme (B) contained in the sterilizing detergent is preferably 0% by mass or more and 10% by mass or less with respect to the mass of the sterilizing detergent from the viewpoint of cleanability. More preferably, it is 0.05% by mass or more and 5% by mass or less, and particularly preferably 0.1% by mass or more and 3% by mass or less.

Examples of the chelating agent (C) include those similar to the chelating agent (C) mentioned in the above-mentioned cleaning agent. As the chelating agent (C), one type alone or two or more types can be used. In one embodiment, the content (mass%) of the chelating agent (C) contained in the sterilizing detergent is 0% by mass or more with respect to the mass of the sterilizing detergent from the viewpoint of the effect of removing protein stains and the cost. It is 0% by mass or less, more preferably 0.005% by mass or more and 2% by mass or less, and further preferably 0.01% by mass or more and 1% by mass or less. As the content of the chelating agent (C), an acid equivalent amount is used.

Examples of the enzyme stabilizer (D) include those similar to the enzyme stabilizer (D) mentioned in the above-mentioned cleaning agent. As the enzyme stabilizer (D), one type alone or two or more types can be used. In one embodiment, the content (mass%) of the enzyme stabilizer (D) contained in the sterilizing detergent is 0% by mass or more and 10% by mass or less with respect to the mass of the sterilizing detergent from the viewpoint of cleanability. It is preferable, more preferably 0.05% by mass or more and 5% by mass or less, and particularly preferably 0.1% by mass or more and 3% by mass or less.

Examples of the anticoagulant (E) include the same as the anticoagulant (E) mentioned in the above-mentioned cleaning agent. As the blood coagulation inhibitor (E), one type alone or two or more types can be used. For example, in one embodiment, the content (% by mass) of glycerin (E-5) contained in the bactericidal detergent is relative to the mass of the bactericidal detergent from the viewpoint of the blood coagulation preventing effect and the coagulating blood dissolving effect. It is 0% by mass or more and 80% by mass or less.

Examples of the metal corrosion inhibitor (F) include the same as the metal corrosion inhibitor (F) mentioned in the above-mentioned cleaning agent. As the metal corrosion inhibitor (F), one type or two or more types can be used. In one embodiment, the content (mass%) of the metal corrosion inhibitor (F) is preferably 0% by mass or more and 10% by mass or less with respect to the mass of the sterilizing detergent from the viewpoint of corrosion suppression performance.

Examples of the low molecular weight polyol (G) include those similar to the low molecular weight polyol (G) mentioned in the above-mentioned cleaning agent. As the low molecular weight polyol (G), one kind or two or more kinds can be used.

In one embodiment, the content (mass%) of the low molecular weight polyol (G) contained in the sterilizing detergent is 0% by mass or more and 80% by mass or less with respect to the mass of the sterilizing detergent from the viewpoint of detergency. preferable.

Examples of the cleaning aid (builder) (H) include the same cleaning aid (builder) (H) mentioned in the above-mentioned cleaning agent. As the builder (H), one type or two or more types can be used. In one embodiment, the content (% by mass) of the builder (H) contained in the sterilizing detergent is preferably 0% by mass or more and 20% by mass or less with respect to the mass of the sterilizing detergent from the viewpoint of detergency.

Examples of the defoaming agent (I) include those similar to the defoaming agent (I) mentioned in the above-mentioned cleaning agent. As the defoaming agent (I), one kind or two or more kinds can be used. In one embodiment, the content (mass%) of the defoaming agent (I) contained in the sterilizing detergent is 0% by mass or more and 10% by mass or less with respect to the mass of the sterilizing detergent from the viewpoint of detergency. preferable.

Examples of the pH adjuster (J) include those similar to the pH adjuster (J) mentioned in the above-mentioned cleaning agent. As the pH adjuster (J), one type or two or more types can be used. In one embodiment, the content (mass%) of the pH adjuster (J) contained in the sterilizing detergent is 0% by mass or more and 25% by mass or less with respect to the mass of the sterilizing detergent from the viewpoint of cleanability. It is preferable, more preferably 0% by mass or more and 15% by mass or less, and particularly preferably 0% by mass or more and 10% by mass or less. In one embodiment, when the pH is on the alkaline side, a salt derived from the cleaning agent remains on the surface of the article to be washed and dried, and a treatment such as wiping may be required to remove the salt. Therefore, depending on the object to be cleaned, the disinfectant cleaning agent of the present disclosure may be preferably acidic, neutral, or weakly alkaline, and may be particularly preferably acidic or neutral. Specifically, the pH (25 ° C.) of the disinfectant detergent of the present disclosure may be preferably less than 9.0, and particularly preferably 7.0 or less. The above does not prevent the use of the disinfectant detergent of the present disclosure outside the pH range. Here, the above pH (25 ° C.) is a value measured by a method based on JIS Z8802: 2011 “pH measurement method”.

The concentration of the fluorinated alcohol when acting on bacteria may be 0.1% by mass or more, preferably 1% by mass or more, further preferably 8% by mass or more, and particularly preferably 29% by mass or more. The time for the fluorinated alcohol to act on the bacteria is not particularly limited. The time for the fluoroalcohol to act on the bacteria is effective even for a short time. For example, the time for allowing the fluorinated alcohol to act on the bacteria may be 30 seconds or longer, preferably 1 minute or longer, more preferably 20 minutes or longer, and particularly preferably 30 minutes or longer.

In one embodiment, the disinfectant detergent may contain the active ingredient of a commercially available disinfectant as an additive. Examples of the active ingredient of the commercially available disinfectant include the same active ingredients of the commercially available disinfectant mentioned in the above-mentioned cleaning agents. In one embodiment, the content (% by mass) of these active ingredients contained in the sterilizing cleaning agent may be 0% by mass or more and 99.9% by mass or less with respect to the mass of the sterilizing cleaning agent, and is 0. It is preferably 0% by mass or more and 99% by mass or less, particularly preferably 0% by mass or more and 92% by mass or less, and further preferably 0% by mass or more and 71% by mass or less.

In one embodiment, the fluoroalcohol contained in the disinfectant detergent can also be used as the disinfectant. Among the fluoroalcohols, HFIP and TFE are particularly suitable. Bacteria in which these fluoroalcohols are effective include, for example, Staphylococcus aureus, Escherichia coli, enterohemorrhagic Escherichia coli, tuberculosis, MRSA, vancomycin-resistant enterococci, multidrug-resistant Pseudomonas aeruginosa, and multidrug-resistant Acinetobacter. However, it is not limited to these.

[Washing method]
The sterilizing detergents containing fluoroalcohols of the present disclosure can be used particularly for cleaning medical devices (including, for example, endoscopes), and further include veterinary medical devices, meat processing tools, and cooking tools. It can be used for cleaning objects. However, the present invention is not limited to this, and can be widely applied to measures against bacterial infection. For example, it can be used for cleaning an operating room in the medical field, linen such as beds and sheets, and cleaning an object including disinfection of human hands.

The sterilizing detergent containing a fluorine-based alcohol of the present disclosure can be used, for example, in the same cleaning method as described in the above-mentioned cleaning method for medical instruments. As the cleaning method, any cleaning method of one type or a combination of two or more types can be used. Here, the concentration of the fluorine-based alcohol contained in the acceptable sterilizing detergent is the same as the concentration at which bacteria are sterilized, and specifically, it may be 0.1% by mass or more, and 1% by mass or more. Is preferable, 8% by mass or more is more preferable, and 29% by mass or more is most preferable.

By using the bactericidal cleaning agent containing a microbial alcohol of the present disclosure in the pre-cleaning step and / or the main cleaning step of the cleaning method, blood, body fluid, fat, prion protein (Prion Protein) derived from a living body can be efficiently derived from an object. Proteins such as PrP) and infectious amyloids, organic substances such as cell tissues, microorganisms, viruses, bacteria and the like can be removed.

However, the disinfectant cleaning agent containing a fluoroalcohol of the present disclosure is not limited to the above-mentioned applications, and may be similar to known antiviral agents, antibacterial agents, disinfectants, disinfectants, fungicides, etc., depending on the object to be cleaned. Can be used. For example, a method of spraying on an object to be cleaned, a method of applying, a method of impregnating the object, a method of immersing the object, a method of exposing the object to high-pressure steam, and the like, which are usually adopted, can be used as they are. ..

The temperature at which the fluorinated alcohol contained in the disinfectant detergent of the present disclosure kills bacteria is not particularly limited, and a temperature of room temperature or higher (for example, 20 ° C. or higher) is preferable. The higher the temperature at which the fluorinated alcohol comes into contact with the bacteria, the easier it is for the bacteria to be sterilized. Further, the temperature may be higher than the boiling point of the fluoroalcohol, that is, vaporized into vapor and brought into contact with bacteria. In addition, the optimum temperature can be selected depending on the solvent and additives contained in the cleaning agent.

[Washing device]
In one embodiment of the cleaning device according to the present invention described above, a sterilizing cleaning agent can be used. By supplying a bactericidal cleaning agent containing the microbial alcohol of the present disclosure, the cleaning apparatus can efficiently remove biological blood, body fluid, fat, prion protein (PrP), infectious amyloid, etc. from the object. Organic substances such as proteins and cell tissues, microorganisms, viruses, bacteria and the like can be removed. The cleaning device can also use a sterilizing cleaning agent containing a fluoroalcohol of the present disclosure and a known cleaning agent in combination.

[Soil cleaner]
The cleaning agent according to the present invention described above can be used as a soil cleaning agent in one embodiment. By using the soil cleaning agent containing the fluoroalcohol of the present disclosure, a bactericidal effect of bacteria contained in the soil can be expected. In one embodiment, the soil cleaner can also include common additives described as known techniques. These also include pesticides, fertilizers, fungicides, disinfectants, etc. used in soil.

The concentration of the fluorinated alcohol when acting on bacteria may be 0.1% by mass or more, preferably 1% by mass or more, further preferably 8% by mass or more, and particularly preferably 29% by mass or more. The time for the fluorinated alcohol to act on the bacteria is not particularly limited. The time for the fluoroalcohol to act on the bacteria is effective even for a short time. For example, the time for allowing the fluorinated alcohol to act on the bacteria may be 30 seconds or longer, preferably 1 minute or longer, more preferably 20 minutes or longer, and particularly preferably 30 minutes or longer. In order to increase the effect, it is also preferable to use a plurality of times in stages with intervals of use on a daily or weekly basis or even on a seasonal basis.

In one embodiment, fluoroalcohols contained in the soil cleaning agent, especially HFIP and TFE, are effective for cleaning soil contaminated with bacteria. HFIP and TFE are stable low molecular weight compounds and have high storage stability. Further, since it has good thermal stability, the cleaning temperature is not limited, and the cleaning power can be further improved. Furthermore, HFIP and TFE are nonflammable, and safety management in use is easy.

[Washing method]
The soil cleaning agent according to the present embodiment can be used to clean soil contaminated with bacteria or soil that may be contaminated. Alternatively, the soil may be washed prophylactically with the soil cleaning agent according to the present embodiment. In one embodiment, the soil cleaning agent may be diluted with the above solvent and used for cleaning. In one embodiment, the soil cleaning agent or solvent and the soil cleaning agent according to the present embodiment are added to the soil to be cleaned, mixed, and the washed soil is separated and dried to sterilize the bacteria and prepare the soil. Can be washed.

Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited to the following Examples as long as the gist of the present invention is not exceeded.

<Cleaning agent>
As a compound that inactivates the virus, HFIP (1,1,1,3,3,3-hexafluoro-2-propanol, Central Glass Co., Ltd., purity 99% or more) is prepared, and EtOH (for disinfection by the Japanese Pharmacopoeia). Ethanol, 80% by volume) compared. In addition, as compounds that inactivate the virus, TFIPL (racemic) (1,1,1-trifluoro-2-propanol, Central Glass Co., Ltd., purity 99% or more), S-TFIPL (1,1,1- Trifluoro-2-propanol, Central Glass Co., Ltd., purity 99% or higher) and R-TFIPL (1,1,1-trifluoro-2-propanol, Central Glass Co., Ltd., purity 99% or higher) were prepared.

<Cells for virus infection>
MDBK cell line (bovine kidney-derived cell line, Madein-Darby bovine kidney cell line, ATCC® CCL-22) as a cell for BVDV infection described later, and CRFK cell line (derived from cat kidney) as a cell for FCV infection described later. Cell line, Crandell Rees feeling kidney cell line, ATCC® CCL-94) and A549 cell line (human alveolar basal epithelial adenocarcinoma-derived cell line, Human Lung) as cells for adenovirus infection, which will be described later.
Epithelial cell line, ATCC® CCL-185) and Vero cell line (monkey kidney-derived cell line, Cercopisecus aethios kidney cell line, ATCC® CCL-81) as cells for enterovirus infection, which will be described later. Four types of cell lines were prepared.

The MDBK cell line uses DMEM (Dulbecco's Modified Eagle's Medium, Sigma Aldrich Japan GK) containing 1% FBS (Fetal Bovine Serum, Sigma Aldrich Japan GK) as a medium. The cells were cultured under humidified conditions of 37 ° C. and 5% CO _2.

The CRFK cell line was humidified using EMEM (Eagle's minimal essential medium, Nissui Pharmaceutical Co., Ltd.) containing 2% FBS as a medium, and cultured under _{37 ° C. and 5% CO 2 conditions.}

The A549 cell line was humidified using DMEM containing 2% FBS as a medium, and cultured under _{37 ° C. and 5% CO 2 conditions.}

Vero cell lines were humidified with EMEM containing 5% FBS as medium and cultured under _{37 ° C. and 5% CO 2 conditions.}

A cell culture dish (AGC Techno Glass Co., Ltd.) and a cell culture microplate (48-well or 96-well, AGC Techno Glass Co., Ltd.) were used as the cell culture container.

MDBK cells or CRFK cells were seeded on a 48-well plate for cell culture, cultured at 37 ° C. _{under 5% CO 2} conditions, and used as BVDV-infected cells or FCV-infected cells.

<Virus>
As RNA viruses having an envelope, bovine viral diarrhea virus (Bovine viral dial virus 1, Strine: NADL, ATCC® VR-534, hereinafter referred to as BVDV), which is an alternative virus to hepatitis C virus, and envelope. As an RNA virus having no virus, a cat-caricivirus (Feline calicivirus, Strine: F-9, ATCC® VR-782, hereinafter referred to as FCV), which is a substitute virus for norovirus, was prepared. Furthermore, as non-enveloped DNA viruses, adenovirus (Human adenovirus 3, Strin: GB, ATCC® VR-3), which is a pathogenic virus of pool fever, and as non-enveloped RNA viruses, are not resistant to drugs. Enterovirus (Human Envelope virus 71, strain: H, ATCC® VR-1432), which is a causative virus of limb and mouth disease, which is said to have high activation resistance, was prepared.

BVDV was infected with the MDBK cell line, and when about 90% or more of the cultured cells showed a cytopathic effect (hereinafter referred to as CPE), the cultured cells were cryopreserved together with the cell culture vessel at −30 ° C. Then, the freeze-thaw operation was performed once, and the supernatant collected by centrifuging at 2380 × g for 10 minutes was used as a preserved virus, and the virus concentrated by the ultrafiltration membrane was used as a preserved virus. The stored virus solution was used as it was as the BVDV test virus solution.

FCV was infected with CRFK cells, and when about 90% or more of the cultured cells showed CPE, the cultured cells were cryopreserved together with the cell culture vessel at −30 ° C. Then, the freeze-thaw operation was performed once, and the supernatant collected by centrifuging at 2380 × g for 10 minutes was concentrated with an ultrafiltration membrane. The virus concentrated by ultracentrifugation using a sucrose cushion was used as a preserved virus. The stored virus solution was diluted 10-fold with DPBS (Dulbecco's Phosphate buffered saline, Nissui Pharmaceutical Co., Ltd.) to prepare an FCV test virus solution.

Adenovirus infects A549 cells, and when about 90% or more of the cultured cells show CPE, the cultured cells were cryopreserved together with the cell culture vessel at −30 ° C. Then, the freeze-thaw operation was performed once, and the supernatant collected by centrifuging at 2380 × g for 10 minutes was concentrated with an ultrafiltration membrane, and the virus prepared by the sucrose density gradient centrifugation method was used as a preserved virus. The stored virus solution was diluted 10-fold with DPBS to prepare an adenovirus test virus solution.

Enterovirus infected Vero cells, and when about 90% or more of the cultured cells showed CPE, the cultured cells were cryopreserved together with the cell culture vessel at −30 ° C. Then, the freeze-thaw operation was performed once, and the supernatant collected by centrifuging at 2380 × g for 10 minutes was used as a preserved virus, and the virus concentrated by the ultrafiltration membrane was used as a preserved virus. The stored virus solution was used as it was as an enterovirus test virus solution.

[Example 1]
HFIP was diluted with distilled water to prepare 100% by mass (stock solution), 80% by mass, 40% by mass, 20% by mass, and 5% by mass of HFIP as test products of Examples 1-1 to 1-5. (In addition, when the volume% is converted into mass% for each test product, it is 100% by mass (stock solution), 86% by mass, 52% by mass, 29% by mass, and 8% by mass, respectively. The same shall apply hereinafter.) 0.9 ml of each of the test products of Examples 1-1 to 1-5 was dispensed into a test tube having a volume of 5 ml, 0.1 ml of the BVDV test virus solution was added and mixed, and the mixture was mixed at room temperature (20 ° C.). It was allowed to act for 30 minutes. The action of the test product was stopped by collecting 0.1 ml from the working solution and adding it to 9.9 ml of SCDLP bouillon medium (Eiken Chemical Co., Ltd.) as an action stopping solution to dilute the test product.

Example 1-1 BVDV test virus solution allowed to act HFIP examples 1-5 from, after 10-fold serial dilutions respectively from 1x to 10 ⁵ fold in DPBS, 2 of 48-well plates seeded with MDBK cells The wells were inoculated with 0.1 ml each (n = 2). After allowing the cells to infect the cells under 37 ° C. and 5% CO ₂ conditions for 1 hour, remove the virus solution and add 0.5 ml of medium per well to 37 ° C. and 5% CO ₂ conditions. Incubated underneath for 4 days.

[Reference example 1]
The procedure was the same as in Example 1 except that ethanol was used as the test product of Reference Example 1 instead of HFIP. Ethanol was diluted with distilled water, and 80% by volume (stock solution) of ethanol was used as the test product of Reference Example 1-1, and 40% by volume of ethanol was used as the test product of Reference Example 1-2.

[Comparative Example 1]
The procedure was the same as in Example 1 except that DPBS was used as the test product of Comparative Example 1 instead of HFIP.

[Example 2]
The procedure was the same as in Example 1 except that FCV and CRFK cells were used as the test virus solution and virus infection cells of Example 2 instead of BVDV and MDBK cells. HFIP was diluted with distilled water to prepare 100% by volume (stock solution), 80% by volume, 40% by volume, 20% by volume, and 5% by volume of HFIP as test products of Examples 2-1 to 2-5. ..

[Reference example 2]
The procedure was the same as in Example 2 except that ethanol was used as the test product of Reference Example 2 instead of HFIP. Ethanol was diluted with distilled water, and 80% by volume (stock solution) of ethanol was used as the test product of Reference Example 2-1 and 40% by volume of ethanol was used as the test product of Reference Example 2-2.

[Comparative Example 2]
The procedure was the same as in Example 2 except that DPBS was used as the test product of Comparative Example 2 instead of HFIP.

[Example 3]
The procedure was the same as in Example 2 except that the action time of the mixture of the HFIP test product of Example 2 and the FCV test virus was shortened from 30 minutes to 1 minute. That is, the only difference between Example 2 and Example 3 is the contact time between HFIP and FCV virus. HFIP was diluted with distilled water to prepare 100% by volume (stock solution), 80% by volume, 40% by volume, 20% by volume, and 5% by volume of HFIP as test products of Examples 3-1 to 3-5. ..

[Reference example 3]
The procedure was the same as in Example 3 except that ethanol was used as the test product of Reference Example 3 instead of HFIP. Ethanol was diluted with distilled water, and 80% by volume (stock solution) of ethanol was used as the test product of Reference Example 3-1 and 40% by volume of ethanol was used as the test product of Reference Example 3-2.

[Comparative Example 3]
The procedure was the same as in Example 3 except that DPBS was used as the test product of Comparative Example 3 instead of HFIP.

[Example 4]
Instead of BVDV and MDBK cells, adenovirus and A549 cells were used as the test virus solution of Example 4 and cells for virus infection, and the same procedure as in Example 1 was carried out except that the action time was 1 minute. HFIP was diluted with distilled water to prepare 100% by volume (stock solution), 80% by volume, 40% by volume, 20% by volume, and 5% by volume of HFIP as test products of Examples 4-1 to 4-5. ..

[Reference example 4]
The procedure was the same as in Example 4 except that ethanol was used as the test product of Reference Example 4 instead of HFIP. Ethanol was diluted with distilled water, and 80% by volume (stock solution) of ethanol was used as the test product of Reference Example 4-1 and 40% by volume of ethanol was used as the test product of Reference Example 4-2.

[Comparative Example 4]
The procedure was the same as in Example 4 except that DPBS was used as the test product of Comparative Example 4 instead of HFIP.

[Example 5]
Instead of BVDV and MDBK cells, enterovirus and Vero cells were used as the test virus solution of Example 5 and cells for virus infection, and the same procedure as in Example 1 was carried out except that the action time was 1 minute. HFIP was diluted with distilled water to prepare 100% by volume (stock solution), 80% by volume, 40% by volume, 20% by volume, and 5% by volume of HFIP as test products of Examples 5-1 to 5-5. ..

[Reference example 5]
The procedure was the same as in Example 5 except that ethanol was used as the test product of Reference Example 5 instead of HFIP. Ethanol was diluted with distilled water, and 80% by volume (stock solution) of ethanol was used as the test product of Reference Example 5-1. Since enterovirus is a virus that is difficult to inactivate, 40% by volume of ethanol was not prepared as a reference example.

[Comparative Example 5]
The procedure was the same as in Example 5 except that DPBS was used as the test product of Comparative Example 5 instead of HFIP.

<Evaluation of virus inactivation by HFIP>
The evaluation of virus inactivation by HFIP was performed by morphological observation using a microscope of cells infected with the virus on which HFIP was allowed to act. Black circles (●) indicate cells in which CPE was confirmed due to virus growth (virus-infected cells), and white circles (○) indicate cells in which CPE could not be confirmed (virus-infected cells), as shown in FIGS. 2 to 6. Shown. Those with cytotoxicity but no CPE due to virus proliferation (cells not infected with virus) are indicated by white triangles (Δ), and cytotoxicity was observed and CPE due to virus proliferation could be confirmed. The cells (virus-infected cells) are indicated by black triangles (▲). Further, "%" in FIGS. 2 to 6 indicates "capacity%".

As shown in FIGS. 2 to 6, the effect of virus inactivation was observed in all the examples and reference examples as compared with the comparative examples.

As shown in FIG. 2, for BVDV, a stronger virus inactivating effect was observed in Examples 1-1 to 1-5 and Reference Example 1-1 as compared with Reference Example 1-2. .. That is, it was shown that HFIP of 5% by volume or more has a stronger effect of BVDV inactivation than 40% by volume EtOH.

As shown in FIG. 3, a stronger virus inactivating effect was observed on FCV in Examples 2-1 to 2-5 and Reference Example 2-1 as compared with Reference Example 2-2. Further, in Examples 2-1 to 2-4, a stronger virus inactivating effect than in Examples 2-5 and Reference Example 2-1 was observed. That is, it was shown that in HFIP of 20% by volume or more, there is a stronger effect of FCV inactivation than in 5% by volume HFIP and 80% by volume EtOH.

As shown in FIG. 4, Examples 3-1 to 3-5 in which the action time of HFIP on FCV was shortened were stronger virus inactivation than Comparative Example 3, Reference Example 3-1 and Reference Example 3-2. It showed an effect. That is, it was shown that in HFIP of 5% by volume or more, there is a stronger effect of FCV inactivation than 40% by volume EtOH and 80% by volume EtOH.

As shown in FIG. 5, for adenovirus, a stronger virus inactivating effect was observed in Examples 4-1 to 4-5 and Reference Example 4-1 as compared with Reference Example 4-2. .. That is, it was shown that HFIP of 5% by volume or more has a stronger adenovirus inactivating effect than 40% by volume EtOH.

As shown in FIG. 6, a stronger virus inactivating effect was observed in Examples 5-1 to 5-5 as compared with Reference Example 5-1 against enterovirus. In particular, a stronger virus inactivating effect was observed in Examples 5-1 to 5-3. That is, in HFIP of 5% by volume or more, there was a stronger adenovirus inactivating effect than in 80% by volume EtOH, and in particular, in HFIP of 40% by volume or more, the inactivating effect was remarkable.

Regarding those in which cytotoxicity was observed in the examples, the presence or absence of cytotoxicity does not affect the cleaning effect, considering that the purpose is to be used for cleaning medical instruments.

[Example 6]
The procedure was the same as in Example 3 except that S-TFIPL was used as the test product of Example 6 instead of HFIP of Example 3. S-TFIPL was diluted with distilled water to prepare 100% by mass (stock solution), 40% by mass, and 20% by mass of S-TFIPL as test products of Examples 6-1 to 6-3 (note that each). When the volume% of the test product is converted to mass%, it is 100% by mass (stock solution), 46% by mass, and 24% by mass, respectively. The same shall apply hereinafter).

[Reference example 6]
The same procedure as in Example 6 was carried out except that ethanol was used as the test product of Reference Example 6 instead of S-TFIPL. Ethanol was diluted with distilled water, and 80% by volume (stock solution) of ethanol was used as the test product of Reference Example 6.

[Comparative Example 6]
The procedure was the same as in Example 6 except that DPBS was used as the test product of Comparative Example 6 instead of S-TFIPL.

[Example 7]
The same procedure as in Example 6 was carried out except that TFIPL (racemic mixture) was used as the test product of Example 7 instead of S-TFIPL. TFIPL (racemic mixture) was diluted with distilled water to prepare 100% by mass (stock solution), 40% by mass, and 20% by mass of TFIPL (racemic mixture) as test products of Examples 7-1 to 7-3. (In addition, when the volume% of each test product is converted into mass%, it is 100% by mass (stock solution), 46% by mass, and 24% by mass, respectively. The same shall apply hereinafter.)

[Reference Example 7]
The same procedure as in Example 7 was carried out except that ethanol was used as the test product of Reference Example 7 instead of TFIPL (racemic mixture). Ethanol was diluted with distilled water, and 80% by volume (stock solution) of ethanol was used as the test product of Reference Example 7.

[Comparative Example 7]
The same procedure as in Example 7 was carried out except that DPBS was used as a test product of Comparative Example 7 instead of TFIPL (racemic mixture).

[Example 8]
S-TFIPL was used as the test product of Example 8 instead of HFIP, and the same procedure as in Example 1 was carried out except that the action time was shortened from 30 minutes to 1 minute. S-TFIPL was diluted with distilled water to prepare 100% by mass (stock solution), 40% by mass, and 20% by mass of S-TFIPL as test products of Examples 8-1 to 8-3 (note that each). When the volume% of the test product is converted to mass%, it is 100% by mass (stock solution), 46% by mass, and 24% by mass, respectively. The same shall apply hereinafter).

[Reference Example 8]
The same procedure as in Example 8 was carried out except that ethanol was used as the test product of Reference Example 8 instead of S-TFIPL. Ethanol was diluted with distilled water, and 80% by volume (stock solution) of ethanol was used as the test product of Reference Example 8.

[Comparative Example 8]
The procedure was the same as in Example 8 except that DPBS was used as the test product of Comparative Example 8 instead of S-TFIPL.

[Example 9]
The same procedure as in Example 8 was carried out except that R-TFIPL was used as the test product of Example 9 instead of S-TFIPL of Example 8. R-TFIPL was diluted with distilled water to prepare 100% by volume (stock solution), 40% by volume, and 20% by volume of R-TFIPL as test products of Examples 9-1 to 9-3.

[Reference example 9]
The same procedure as in Example 9 was carried out except that ethanol was used as the test product of Reference Example 9 instead of R-TFIPL. Ethanol was diluted with distilled water, and 80% by volume (stock solution) of ethanol was used as the test product of Reference Example 9.

[Comparative Example 9]
The procedure was the same as in Example 9 except that DPBS was used as a test product of Comparative Example 9 instead of R-TFIPL.

[Example 10]
The same procedure as in Example 9 was carried out except that TFIPL (racemic mixture) was used as the test product of Example 10 instead of R-TFIPL of Example 9. The TFIPL (racemic mixture) was diluted with distilled water to prepare 100% by volume (stock solution), 40% by volume, and 20% by volume of TFIPL (racemic mixture) as the test products of Examples 10-1 to 10-3.

[Reference Example 10]
The same procedure as in Example 10 was carried out except that ethanol was used as the test product of Reference Example 10 instead of TFIPL (racemic mixture). Ethanol was diluted with distilled water, and 80% by volume (stock solution) of ethanol was used as the test product of Reference Example 10.

[Comparative Example 10]
The same procedure as in Example 10 was carried out except that DPBS was used as a test product of Comparative Example 10 instead of TFIPL (racemic mixture).

<Evaluation of virus inactivation by TFIPL>
The evaluation of virus inactivation by TFIPL was performed by morphological observation using a microscope of cells infected with the virus on which TFIPL was allowed to act. Black circles (●) indicate cells in which CPE was confirmed due to virus growth (virus-infected cells), and white circles (○) indicate cells in which CPE could not be confirmed (virus-infected cells), as shown in FIGS. 7 to 11. Shown. Those with cytotoxicity but no CPE due to virus proliferation (cells not infected with virus) are indicated by white triangles (Δ), and cytotoxicity was observed and CPE due to virus proliferation could be confirmed. The cells (virus-infected cells) are indicated by black triangles (▲). Further, "%" in FIGS. 7 to 11 indicates "capacity%".

As shown in FIGS. 7 to 11, the effect of virus inactivation was observed in all the examples and reference examples as compared with the comparative examples.

As shown in FIG. 7, it was confirmed that Examples 6-1 to 6-3 in which S-TFIPL was allowed to act on FCV had a stronger virus inactivating effect as compared with Reference Example 6. That is, it was shown that S-TFIPL of 20% by volume or more has a stronger effect of FCV inactivation than 80% by volume EtOH.

As shown in FIG. 8, it was confirmed that Examples 7-1 to 7-3 in which TFIPL (racemic mixture) was allowed to act on FCV had a stronger virus inactivating effect as compared with Reference Example 7. In particular, a stronger virus inactivating effect was observed in Examples 7-1 and 7-2. That is, it was shown that in TFIPL (racemic mixture) of 20% by volume or more, there is a stronger effect of FCV inactivation than 80% by volume EtOH.

As shown in FIG. 9, it was confirmed that Examples 8-1 to 8-3 in which S-TFIPL was allowed to act on BVDV had a stronger virus inactivating effect as compared with Comparative Example 8. That is, it was shown that there is an effect of BVDV inactivation in S-TFIPL of 20% by volume or more.

As shown in FIG. 10, it was confirmed that Examples 9-1 to 9-3 in which R-TFIPL was allowed to act on BVDV had a stronger virus inactivating effect as compared with Comparative Example 9. That is, it was shown that there is an effect of BVDV inactivation in R-TFIPL of 20% by volume or more.

As shown in FIG. 11, it was confirmed that Examples 10-1 to 10-3 in which TFIPL (racemic mixture) was allowed to act on BVDV had a stronger virus inactivating effect as compared with Comparative Example 10. That is, it was shown that there is an effect of BVDV inactivation in TFIPL (racemic mixture) of 20% by volume or more.

[Protein solubility test]
HFIP is diluted with distilled water, and using 100% by volume (stock solution), 80% by volume, 40% by volume, 20% by volume, and 5% by volume of HFIP aqueous solution, a cleaning evaluation indicator for medical equipment (TOSI, Matsuyoshi Medical Instruments) Cleaning evaluation of human plasma protein pseudocontamination was carried out using (manufactured by Co., Ltd.). After immersing TOSI in an HFIP aqueous solution of each concentration and performing ultrasonic cleaning at 20 ° C. for 10 minutes, the presence or absence of residual protein on TOSI was confirmed with a residual protein detection solution (manufactured by Saraya Co., Ltd., Power Quick). However, no residual protein was detected when any concentration of HFIP aqueous solution was used. That is, it was found that the pseudo-contamination on TOSI can be removed in HFIP of 5% by volume or more.

[Bactericidal evaluation test]
<Inoculated strain>
As the strain, Escherichia cоli NBRC3972 strain (Escherichia coli) was used.

<Measuring medium>
A trypticase soy agar medium (Becton, Dickinson) was used as the measurement medium.

<Preparation of evaluation solution>
The evaluation sample was diluted with sterile water and adjusted to the desired concentration.

<Preparation of inoculum solution>
After culturing overnight cells in front medium, scraped the cells, so that the 10 ⁸ cfu / mL, using 0.1 wt.% By volume of tryptone containing 0.85 wt% by volume of saline , Inoculated bacterial solution was prepared.

[Example 11-1]
Take 10 μL of the inoculum solution and dispense it into a microtube. Add evaluation sample solution: 80% HFIP to a 190 μL microtube and let it act for 30 seconds, then take 10 μL of the reaction solution and contain 0.1 wt / volume% tryptone. It was diluted 100-fold with .85% by volume of physiological saline to stop the action of the sample (the solution is called a reaction stop solution). The reaction terminator was serially diluted 10-fold to 10,000-fold with 0.85 wt% by weight saline containing 0.1 wt% tryptone. 100 μL was dispensed from each diluted solution and applied to each pre-medium. They were cultured overnight in an incubator at 35 ° C., and the number of colonies grown with the naked eye was measured.

<Bactericidal evaluation method>
The common logarithmic value of the viable cell count (CFU / mL) contained in the inoculated bacterial solution and the average value thereof were determined. The logarithmic reduction value (Log Reduction Value: LR value) was calculated from the average value of this average value and the logarithmic average value of the viable cell count (CFU / mL) after the action of the evaluation sample solution by the following formula.
Log Reduction = AB
A: Average number of viable bacteria (common logarithmic value) contained in the inoculated bacterial solution B: Average value of viable cell count (common logarithmic value) after the action of the evaluation sample solution

[Example 11-2]
The bactericidal evaluation test was carried out in the same procedure as in Example 11-1 except that the evaluation sample solution was 40% HFIP.

[Example 11-3]
The bactericidal evaluation test was carried out in the same procedure as in Example 11-1 except that the evaluation sample solution was 20% HFIP.

[Example 11-4]
The bactericidal evaluation test was carried out in the same procedure as in Example 11-1 except that the evaluation sample solution was 10% HFIP and the reaction terminator solution was diluted up to 1,000 times every 10 times.

[Example 11-5]
The bactericidal evaluation test was carried out in the same procedure as in Example 11-1 except that the evaluation sample solution was 5% HFIP and the reaction terminator solution was diluted up to 1,000 times every 10 times.

[Example 11-6]
The bactericidal evaluation test was carried out in the same procedure as in Example 11-1 except that the evaluation sample solution was 2.5% HFIP and the reaction terminator solution was diluted up to 1,000 times every 10 times.

[Example 12-1]
The bactericidal evaluation test was carried out in the same procedure as in Example 11-1 except that the evaluation sample solution was 80% TFE (85% by mass TFE when the volume% was converted to mass%. The same applies hereinafter). Was carried out.

[Example 12-2]
The bactericidal evaluation test was carried out in the same procedure as in Example 12-1 except that the evaluation sample solution was set to 40% TFE (in addition, when volume% is converted to mass%, it is 48% by mass TFE. The same shall apply hereinafter). Was carried out.

[Example 12-3]
The bactericidal evaluation test was carried out in the same procedure as in Example 12-1 except that the evaluation sample solution was set to 20% TFE (Note that when volume% is converted to mass%, it is 26% by mass TFE. The same applies hereinafter). Was carried out.

[Example 12-4]
The evaluation sample solution was set to 10% TFE (Note that when volume% is converted to mass%, it is 13% by mass TFE. The same applies hereinafter), and the reaction terminator solution was diluted to 1,000 times every 10 times. Except for the above, the bactericidal property evaluation test was carried out in the same procedure as in Example 12-1.

[Comparative Example 11]
The bactericidal evaluation test was carried out in the same procedure as in Example 11-1 except that the evaluation sample solution was a physiological saline solution.

[Reference Example 11-1]
The bactericidal evaluation test was carried out in the same procedure as in Example 11-1 except that the evaluation sample solution was 80% EtOH.

[Reference Example 11-2]
The bactericidal evaluation test was carried out in the same procedure as in Example 11-1 except that the evaluation sample solution was 40% EtOH.

[Reference Example 11-3]
The bactericidal evaluation test was carried out in the same procedure as in Example 11-1 except that the evaluation sample solution was 20% EtOH.

Concentration of inoculum, evaluation sample solution, contact with respect to Examples 11-1 to 11-6, Examples 12-1 to 12-4, Comparative Example 11 and Reference Example 11-1 to 11-3. FIG. 12 shows a summary of the number of colonies after culturing at each dilution ratio over time. In FIG. 12, "-" indicates that it has not been implemented. In addition, FIG. 13 shows the bactericidal efficacy of each evaluation sample solution by LR value (Log Reduction Value). For each evaluation sample solution, those having an LR value of 3.0 or more (the viable cell count decreased by 99.9% or more) were designated as "◎", and those having an LR value of less than 1.0 were designated as "x". Judged.

As shown in FIG. 13, it is clear that HFIP and TFE have a bactericidal effect on Escherichia coli. In particular, from the comparison between Reference Example 11-3 and Example 11-3 and the comparison between Reference Example 11-3 and Example 12-3, it can be seen that HFIP and TFE show stronger bactericidal activity than EtOH. In this example, although it showed a bactericidal effect on Escherichia coli, fluoroalcohols such as HFIP and TFE can be used for other bacteria such as Staphylococcus aureus, Escherichia coli, enterohemorrhagic Escherichia coli, Mycobacterium tuberculosis, MRSA, and vancomycin-resistant enterococci. , Multidrug-resistant Staphylococcus aureus, multidrug-resistant Acinetobacter, etc. are also considered to show the same bactericidal effect.

The cleaning agent that inactivates the virus in the present disclosure is usefully used for cleaning medical instruments and the like.

1 ... cleaning device, 10 ... cleaning tank, 12 ... water storage unit, 14 ... water supply pipe, 16 ... hot water supply pipe, 18 ... cleaning pump, 20 ... storage unit, 22・・・ Cleaning nozzle, 34 ・・・ Drain pipe, 40 ・・・ Cleaning agent supply device

Claims

A cleaning agent containing a fluorine-based alcohol as a compound that inactivates viruses.
The fluorinated alcohol is represented by the general formula RfCH 2 OH or RfRf'CHOH.
Rf and Rf'represent a perfluoroalkyl group having 1 to 10 carbon atoms.
The cleaning agent according to claim 1, wherein Rf and Rf'are different from each other or are the same.
The fluoroalcohols are 1,1,1,3,3,3-hexafluoro-2-propanol, 2,2,2-trifluoroethanol and 2,2,3,3,3-pentafluoro-1-. The cleaning agent according to claim 1, which is at least one selected from the group consisting of propanol.
The cleaning agent according to claim 1, wherein the concentration of the fluoroalcohol when acting on the virus is 0.1% by mass or more.
The cleaning agent according to claim 1, further comprising a solvent.
The cleaning agent according to claim 1, wherein the virus is at least one selected from the group consisting of influenza virus, hepatitis C virus, rotavirus, norovirus, adenovirus and enterovirus.
The cleaning agent according to claim 1, which is used for cleaning medical instruments.
The cleaning agent according to claim 1, which is used for cleaning soil.
A cleaning method that involves the action of fluorine-based alcohol on the virus.
Claim that the fluoroalcohol is represented by the general formula RfCH 2 OH or RfRf'CHOH, Rf and Rf'represent a perfluoroalkyl group having 1 to 10 carbon atoms, and Rf and Rf'are different from each other or are the same. Item 9. The cleaning method according to Item 9.
The fluoroalcohols are 1,1,1,3,3,3-hexafluoro-2-propanol, 2,2,2-trifluoroethanol and 2,2,3,3,3-pentafluoro-1-. The cleaning method according to claim 9, which is at least one selected from the group consisting of propanol.
The cleaning method according to claim 9, wherein the concentration of the fluorinated alcohol when acting on the virus is 0.1% by mass or more.
The cleaning method according to claim 9, wherein the fluorinated alcohol is diluted with a solvent.
The cleaning method according to claim 9, wherein the virus is at least one selected from the group consisting of influenza virus, hepatitis C virus, rotavirus, norovirus, adenovirus and enterovirus.
The cleaning method according to claim 9, which is used for cleaning medical instruments.
The cleaning method according to claim 9, which is used for cleaning soil.
A cleaning device including a cleaning agent supply device that supplies a cleaning agent containing a fluoroalcohol as a compound that inactivates a virus.
Claim that the fluoroalcohol is represented by the general formula RfCH 2 OH or RfRf'CHOH, Rf and Rf'represent a perfluoroalkyl group having 1 to 10 carbon atoms, and Rf and Rf'are different from each other or are the same. Item 17. The cleaning device according to item 17.
The fluoroalcohols are 1,1,1,3,3,3-hexafluoro-2-propanol, 2,2,2-trifluoroethanol and 2,2,3,3,3-pentafluoro-1-. The cleaning device according to claim 17, which is at least one selected from the group consisting of propanol.
The cleaning device according to claim 17, wherein the concentration of the fluorinated alcohol when acting on the virus is 0.1% by mass or more.
The cleaning device according to claim 17, wherein the fluorinated alcohol is diluted with a solvent.
The cleaning device according to claim 17, wherein the virus is at least one selected from the group consisting of influenza virus, hepatitis C virus, rotavirus, norovirus, adenovirus and enterovirus.
The cleaning device according to claim 17, which is used for cleaning medical instruments.
The cleaning device according to claim 17, which is used for cleaning soil.
A cleaning agent containing fluoroalcohol as a compound that kills bacteria.
The fluorinated alcohol is represented by the general formula RfCH 2 OH or RfRf'CHOH.
Rf and Rf'represent a perfluoroalkyl group having 1 to 10 carbon atoms.
25. The cleaning agent according to claim 25, wherein Rf and Rf'are different from each other or are the same.
The fluoroalcohols are 1,1,1,3,3,3-hexafluoro-2-propanol, 2,2,2-trifluoroethanol and 2,2,3,3,3-pentafluoro-1-. The cleaning agent according to claim 25, which is at least one selected from the group consisting of propanol.
The cleaning agent according to claim 25, wherein the concentration of the fluoroalcohol when acting on bacteria is 0.1% by mass or more.
The cleaning agent according to claim 25, further comprising a solvent.
The cleaning agent according to claim 25, which is used for cleaning medical instruments.
The cleaning agent according to claim 25, which is used as a disinfectant.
The cleaning agent according to claim 25, which is used for cleaning soil.
A cleaning method that involves the action of fluorinated alcohol on bacteria.
Claim that the fluoroalcohol is represented by the general formula RfCH 2 OH or RfRf'CHOH, Rf and Rf'represent a perfluoroalkyl group having 1 to 10 carbon atoms, and Rf and Rf'are different from each other or are the same. Item 33. The cleaning method according to Item 33.
The fluoroalcohols are 1,1,1,3,3,3-hexafluoro-2-propanol, 2,2,2-trifluoroethanol and 2,2,3,3,3-pentafluoro-1-. The cleaning method according to claim 33, which is at least one selected from the group consisting of propanol.
The cleaning method according to claim 33, wherein the concentration of the fluorinated alcohol when acting on the bacteria is 0.1% by mass or more.
The cleaning method according to claim 33, wherein the fluorinated alcohol is diluted with a solvent.
The cleaning method according to claim 33, which is used for cleaning medical instruments.
The cleaning method according to claim 33, which is used for cleaning soil.
A cleaning device including a cleaning agent supply device that supplies a cleaning agent containing a fluoroalcohol as a compound that kills bacteria.
Claim that the fluoroalcohol is represented by the general formula RfCH 2 OH or RfRf'CHOH, Rf and Rf'represent a perfluoroalkyl group having 1 to 10 carbon atoms, and Rf and Rf'are different from each other or are the same. Item 40. The cleaning device according to item 40.
The fluoroalcohols are 1,1,1,3,3,3-hexafluoro-2-propanol, 2,2,2-trifluoroethanol and 2,2,3,3,3-pentafluoro-1-. The cleaning device according to claim 40, which is at least one selected from the group consisting of propanol.
The cleaning device according to claim 40, wherein the concentration of the fluorinated alcohol when acting on the bacteria is 0.1% by mass or more.
The cleaning device according to claim 40, wherein the fluorinated alcohol is diluted with a solvent.
The cleaning device according to claim 40, which is used for cleaning medical instruments.
The cleaning device according to claim 40, which is used for cleaning soil.