WO2021104499A1

WO2021104499A1 - Sample adding and mixing method and apparatus, and non-transitory computer readable storage medium

Info

Publication number: WO2021104499A1
Application number: PCT/CN2020/132465
Authority: WO
Inventors: 赖华忠
Original assignee: 深圳市帝迈生物技术有限公司
Priority date: 2019-11-29
Filing date: 2020-11-27
Publication date: 2021-06-03
Also published as: CN112871029B; CN112871029A

Abstract

A sample adding and mixing method, a sample adding and mixing apparatus, and a non-transitory computer readable storage medium. The sample adding and mixing method comprises the following steps: placing a sample setting part (2) at a suspended position (3); adding substances to be added into the sample setting part (2) by a sample adding part (5); and before the sample setting part (2) reaches a test position (4), performing a mixing operation on the sample setting part (2) by means of a transfer part (6), so that the substances added into the sample setting part (2) are well mixed to obtain a substrate to be tested. The method can effectively shorten the time from a mixing step to a test step.

Description

Sample adding and mixing method and device, and non-temporary computer readable storage medium

【Technical Field】

This application relates to the technical field of medical inspection, and specifically relates to a sample adding and mixing method and device, and a non-temporary computer-readable storage medium.

【Background technique】

In the field of medical inspection technology, it is a common application scenario to add samples and reagents to the sample setting part for reaction, detection, processing and/or analysis, and to mix them. Among them, the mixing operation is a key link in the above process.

[Summary of the invention]

In response to the above problems, this application develops a sample adding and mixing method that can effectively shorten the time from the mixing link to the detection link. At the same time, it also provides a sample adding and mixing device that can realize the above method, which is non-temporary. Computer readable storage medium.

One of the technical means adopted in this application is to provide a sample adding and mixing method, which includes the following steps: placing the sample placing part on a suspended position; adding the sample adding part to the sample placing part; Before the sample portion reaches the detection position, the sample placement portion is mixed by the transfer portion, so that the additives in the sample placement portion are mixed uniformly to obtain the test object.

Another technical means adopted in this application is to provide a sample adding and mixing device, comprising: a sample adding part and a transplanting part, wherein the sample adding part is used when the sample placing part is in a suspended position, Add objects to be added to the sample setting part; the transfer part is used to move the sample setting part from the placing position to the suspended position, and perform a mixing operation on the sample setting part to make the sample setting part The additives in the part are mixed to obtain the test object, and the sample placement part is moved to the detection position to detect the test object; wherein, the distance between the placement position and the detection position Greater than the distance between the suspended position and the detection position.

Another technical means adopted in this application is to provide a non-transitory computer-readable storage medium, wherein the non-transitory computer-readable storage medium stores a computer program, and when the computer program is executed by a processor, The processor executes the following steps: placing the sample placing part in a suspended position through the transplanting part; adding the sample adding part to the sample placing part; before the sample placing part reaches the detection position, by transferring The part performs a mixing operation on the sample setting part, so that the additives in the sample setting part are mixed uniformly to obtain the test object.

In the above technical solution, the sample setting part is first placed in a suspended position, and then the object to be added is added to the sample setting part, and before the sample setting part reaches the detection position, the sample setting part is mixed by the transfer part. So that the additives in the sample setting part are mixed evenly. Therefore, there is no need to wait for the sample setting part to perform the mixing operation after reaching the detection position, thereby shortening the time from the mixing step to the detection step.

【Explanation of the drawings】

In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings that need to be used in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only These are some embodiments of the present application. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative work.

among them:

Figure 1 is a flow chart of a sample adding and mixing method in an embodiment;

Figure 2 is a flow chart of a sample adding and mixing method in an embodiment;

Figure 3 is a flow chart of a sample adding and mixing method in an embodiment;

Fig. 4 is a flow chart of the steps of the sample adding part adding sample to the sample placing part in an embodiment;

Figure 5 is a partial flow chart of a sample adding and mixing method in an embodiment;

Fig. 6A, Fig. 6B, Fig. 6C, Fig. 6D are diagrams of implementation examples of the sample adding and mixing method;

Figure 7 is a partial flow chart of a sample adding and mixing method in an embodiment;

Figure 8 is a diagram of an implementation example of moving the sample setting part to the detection position;

Figure 9 is a partial flow chart of a sample adding and mixing method in an embodiment;

Figure 10 is a diagram showing an example of an implementation of moving the sample setting part from the placement position to the suspended position;

Figure 11 is a structural block diagram of a sample adding and mixing device in an embodiment;

Figure 12 is a structural block diagram of a sample adding and mixing device in an embodiment;

Fig. 13 is a structural block diagram of a non-transitory computer-readable storage medium in an embodiment.

【Detailed ways】

In order to make the purpose of the invention, technical solutions and technical effects of the present application clearer, the following further describes the present application in detail with reference to the accompanying drawings and specific implementations. It should be understood that the specific implementations described in this specification are only for explaining the application, not for limiting the application. In the case of no conflict, the embodiments in the application and the features in the embodiments can be combined with each other.

This application provides a sample adding and mixing method, as shown in FIG. 1. In one embodiment, the sample adding method may include the following steps:

Step S12: Place the sample setting part in a suspended position.

Wherein, when the sample setting part is in a suspended position, the sample setting part is in a suspended state.

The sample placement part refers to a container in which samples or reagents can be placed, and it can be a sample container, a reagent container, such as a reaction cup, a test tube, and the like. The specific type and form of the sample setting part have no influence on the sample adding and mixing method claimed in this application. The suspended state of the sample placing part means that the bottom of the sample placing part is not placed on any bearing surface, and the sample placing part in the suspended position can be clamped or fixed by the transfer part to keep the sample placing part suspended. The transfer part may be a part or structure with a fixing function or a clamping function such as a manipulator, a finger cylinder, a gripper, etc.; in one embodiment, the suspended position may be located above the placement position, above the detection position, or between the placement position and the detection position. between. The placement position may be the position where the sample placement part is placed before being placed in the suspended position. For example, in sample analysis, the placement position can be a position for placing an empty sample placement part, or it can be used as a pre-sampling position, that is, first add one or part of a sample or reagent to the sample placement part at the placement position. The sample placement part where the sample or reagent has been added to the pre-loading position can also be incubated before moving to the suspended position. The suspended position can be a position that is convenient for the sample application part to reach and move the sample setting part to the detection position. For example, the selection of the suspension position can be based on the distance between the suspended position and the detection position, the distance between the suspended position and the initial position of the sampling part, and the suspended position The distance from the initial position of the sample setting part is determined, specifically, it may be the suspended position corresponding to the case where the sum of the above three distances is the shortest. The detection position can be a position where the sample setting part can be placed and the test object in the sample setting part can be detected. The test object can be the object to be added by the sample adding part in the following step S14, or it can be in the following step S14 The samples and reagents placed in the pre-sample section may also be substances obtained by reacting the object to be added with other samples and reagents in the pre-sample section.

Step S14: the sample adding part adds the object to be added into the sample placing part.

The sample adding part can be a sample needle, or other parts capable of aspirating samples or reagents. In step S14, the substance to be added by the sample adding part to the sample placing part can be only the sample, or only the reagent, or both the sample and the reagent are added. If only the sample is added, the reagent needs to perform the following step S16 Add at the time or before. If only the reagent is added, the sample needs to be added before step S14. For example, the sample can be added to the sample setting part in the setting area of the sample setting part, and then the sample setting part can be moved to the suspension position. Of course, the way that the sample setting part moves in the front and the sample application part is loaded after is compared to the method where the sample setting part is moved before and the sample setting part moves behind, which reduces the movement of the sample setting part that has already been loaded. Time helps reduce the occurrence of sample contamination. When the sample addition and mixing method is applied to sample analysis, the sample can be blood-related samples, such as blood, blood components, etc., blood components can be plasma, blood cells, etc.; reagents can be fibrinolysis, antifibrinolysis, coagulation, and anticoagulation And other detection reagents. In addition, the sample adding section can add objects to be added to the sample placing section in a moving state, or add objects to be added to the sample placing section in a suspended state, that is, when the sample adding section adds objects to be added to the sample placing section, the two People can be in relative motion or relatively static state.

Step S16: Before the sample setting part reaches the detection position, a mixing operation is performed on the sample setting part through the transfer part, so that the additives in the sample setting part are mixed uniformly.

Wherein, under the action of the transfer part, the sample setting part has at least one of a moving state and a mixing state.

As mentioned above, the transfer part can be a part or structure with a clamping function, such as a manipulator, a finger cylinder, or a clamping jaw. It can drive the sample setting part to move, for example, transfer the sample setting part to the suspended position, transfer to the detection position, or drive the sample setting part to be in a state of mixing, and then face the objects to be added in the sample setting part. Shaking is performed, for example, the sample and/or reagent are mixed, for example, the blood-related sample and the detection reagent can be shaken. Of course, under the action of the transfer part, the sample placement part can be in both a moving state and a mixing state, for example, mixing while moving. Under the action of the transfer part, the sample setting part can also be kept in the suspended position for a predetermined time, and the predetermined time can be the time required to implement step S14 or step S16.

In an embodiment, the position where the sample setting part is in the mixing state may be a suspended position, or any position that the sample setting part can pass during the process of moving from the suspended position to the detection position. The specific mixing position of the sample setting part can be the suspended position. In this case, the sample addition part also directly adds the substance to be added to the sample setting part located in the suspended position, and directly adjusts the sample in the suspended position after the sample is added. The sample setting part performs a mixing operation to mix the additives in the sample setting part evenly. The position where the sample setting part is specifically mixed can be any position that can be passed during the process of moving the sample setting part from the suspended position to the detection position, that is, the sample setting part can be transferred from the suspended position to the detection position in the transfer part During the process of mixing the sample setting part, there can be multiple moving paths from the suspended position to the detection position, as long as the sample setting part can be moved from the suspended position to the detection position. In addition, the sample setting part can keep moving during the moving process, of course, it can also pause after moving a certain path, and then continue to enter the moving state after pausing for a certain period of time. The moving path and the state of the sample setting part during the moving process can be set according to requirements. For example, the sample setting part can reach the detection position through moving path 1 from the suspended position, or reach the detection position through moving path 2, or reach the detection position through other moving paths. Any point on any moving path can be opposite. The position where the sample part performs the mixing operation, of course, this position may be above the detection position where the sample part can be accommodated. The implementation position of the mixing operation can be determined according to the best detection time of the object to be added this time.

In one embodiment, when the sample setting part is mixed by the transfer part, the sample setting part clamped by the transfer part can be used to apply a driving force that enables the sample setting part to perform the mixing action.

The driving force includes at least one of a rotation force, a swing force and a vibration force, and the sample setting part realizes rotation mixing, swing mixing, vibration mixing, or other mixing actions. Of course, the rotational force, swing force, and vibration force applied by the transfer part can appear superimposed or appear in sequence, for example, the sample placement part sometimes performs rotation mixing and sometimes vibration mixing. At the same time, the duration of the mixing action can also be adjusted according to the actual situation.

It should be pointed out that in related technologies, samples can be mixed in the following two ways.

One is that the mixing mechanism is set in the placement area of the sample placement part. After the sample and reagent loading operations are completed, they are directly mixed at the placement location, and then the sample is detected at the detection location. Since the sample placement part needs to move from the placement area to the detection position after mixing, it takes a long time and it is easy to miss the optimal detection time of the sample, especially for the quick response sample detection process. This mixing method has an adverse effect on the inspection speed and performance.

The other is that the mixing mechanism is set at the detection position of the sample, and the sample and reagent are transferred to the detection position after the sample and reagent addition operation is completed, and the mixing is performed at the detection position. The structure of this mixing method is relatively complicated, and for the unified detection and analysis of multiple samples, a mixing mechanism needs to be added to each detection channel, which significantly increases the cost. In the sample adding and mixing method in this embodiment, the sample placing part is first placed in a suspended position, and then the object to be added is added to the sample placing part, and then the sample placing part is mixed by the transfer part. The position where the sample setting part is mixed can be the position where the sample setting part is in the mixing state, such as a suspended position, or any position that the suspended position can pass during the process of moving to the detection position, so that the sample addition and mixing method can be realized. An additional mixing mechanism is provided in the placement area or detection position of the sample placement part, which can effectively reduce costs; at the same time, the sample placement part located in the suspended position is mixed and moved after sample addition, which is convenient to reduce the movement of the sample placement part to the detection position. Time, also avoids the problem of missing the best detection time after mixing, which is beneficial to improve the detection efficiency and the accuracy of the detection results. When applied to sample analysis equipment, such as chemiluminescence analyzers, coagulation analyzers and other clinical testing equipment, it can effectively avoid the problem of missing the best sample analysis and detection time on the basis of cost control, which is conducive to obtaining More accurate sample analysis results. In the case where the position where the sample setting part is in the mixing state is a suspended position, or a position between the suspended position and the detection position, because the sample setting part is suspended and mixed, it can also effectively avoid the sample setting part and its bearing surface. The problem of collision.

In one embodiment, as shown in Figures 2 and 3, after the step of adding the object to be added to the sample placing part by the sample adding part, the sample adding and mixing method may further include the following steps:

Step S20: The transfer part moves the sample setting part to the detection position.

Specifically, as shown in FIG. 2, step S20 can be performed after step S16, that is, the sample setting part transferred to the detection position has completed the operations of adding the object to be added and mixing the object to be added, then the sample setting part is directly Transfer to the detection position for sample detection. Specifically, as shown in FIG. 3, step S20 can also be performed between step S14 and step S16, that is, the sample placement part that has been transferred to the detection position has passed the sample addition of the object to be added but has not performed the mixing operation, and is transferred to After detecting the position, firstly mix the sample placement part through the transfer part, and then perform the sample detection. The two methods can be selected according to the actual sample reaction and analysis requirements.

In one embodiment, referring to FIG. 4, the method for adding and mixing samples may further include the following steps:

Step S32: Detect whether the sample setting part is placed on the transfer part.

Specifically, a sensor, a sensing element, a detection component, etc. can be used to detect whether the sample placement part is clamped on the transfer part.

Step S34: When the sample setting part is placed on the transfer part, the current position of the sample setting part is acquired.

For example, it is known that the sample setting part is currently located in the suspended position, the detection position, etc. Specifically, the current position of the sample setting part can be obtained by detecting the specific position of the transfer part holding the sample setting part. When the sample setting part is not placed on the transfer part, the transfer part can be controlled to perform the clamping operation of the sample setting part.

Step S36: According to the current position of the sample setting part, the state of the sample setting part is controlled by the transfer part.

Through the above detection and acquisition results, it is convenient to control and adjust the state of the sample setting part, such as controlling the sample setting part to be in a suspended state, a moving state, a mixing state, and so on. For example, when the sample setting part is clamped on the transfer part but has not reached the suspended position, the transfer part can be used to control the sample setting part to be in a moving state. When the sample setting part is clamped on the transfer part and reaches the suspended position, the sample setting part can be controlled to be in a suspended state, and at the same time, the sample adding part can be controlled to perform the sample adding work. After a predetermined time, the sample setting part can be controlled to enter the mixed position. Uniform state or mobile state, etc.

In an embodiment, as shown in FIG. 5, the step S14 of adding the object to be added by the sample adding part to the sample placing part may include:

Step S142: the sample adding part and the sample setting part are close to each other.

The sample setting part before sample addition can be an empty sample setting part, that is, before sample addition, there is no liquid such as sample or reagent in the sample setting part, and the sample setting part before sample addition can also be filled with liquid in advance, that is, to the existing liquid Add samples or reagents to the sample setting section. Before adding samples, you can control the sample adding part to move to the position of the sample setting part, the position of the sample setting part can be a suspended position; you can also control the sample setting part to move to the sample part, and you can also control the sample adding part and the sample setting part. Both move towards each other.

Step S144: the sample adding part adds the object to be added to the sample placing part at the sample adding position.

The sample adding position refers to the position where the sample adding part is when adding the object to be added, and the sample adding position can be set in advance.

The objects to be added can be samples, reagents, etc., if the sample adding part is a sample needle, the objects to be added can be discharged from the sample ejection port of the sample needle.

Step S146: After the sample application is completed, the sample application part and the sample setting part are far away from each other.

After the sample is added, you can control the sample adding part to move away from the sample placing part, you can also control the sample placing part to move away from the sample adding part, and you can also control both the sample adding part and the sample placing part to move away from each other.

As shown in FIG. 6B and FIG. 6C, the sample adding part 5 moves to the sample placing part 2 placed on the suspended position 3 to add samples.

In one embodiment, after the objects to be added are discharged, the first state or the second state may be satisfied between the sample adding portion 5 at the sample adding position and the liquid level in the sample placing portion 2. Among them, the first state is that the liquid contact position of the sample adding section 5 just touches the liquid surface in the sample placing section 2, that is, the two are at the same height level; the second state is that the liquid contact position of the sample adding section 5 is located at the sample placing section At a certain distance above the liquid surface in 2 and the distance from the liquid surface in the sample placement part 2 is less than a preset distance. As shown in Figure 6B, after the sample adding part 5 discharges the objects to be added, the liquid contact position of the sample adding part 5 is located above the liquid surface 7 in the sample placing part 2 and the distance from the liquid surface 7 in the sample placing part 2 If the distance is less than the preset distance, the liquid level 7 in the sample adding portion 5 and the sample placing portion 2 is in the second state; as shown in Fig. 6C, the sample adding portion 5 touches the liquid after the object to be added is discharged. The position just touches the liquid surface 7 in the sample setting part 2, that is, the liquid surface 7 in the sample setting part 2 is in the first state between the sample application part 5 and the liquid surface 7 in the sample setting part 2.

The liquid contact position of the sample adding part can be any position between the front end of the sample adding part and the discharge port of the sample adding part, and the front end is close to the discharge port. Specifically, it can be the front end, the discharge port, or the front end. And the middle position between the sample port and so on.

The preset distance can be the height of a naturally falling sample drop formed when the sample dispensing part performs the sample discharge operation. The preset distance can be obtained through prior experiments. For example, the sample dispensing part is used to perform the sample drop operation, and the formation is obtained by measurement. The height value of a sample droplet that falls naturally can be used as the value of the preset distance. In practical applications, the preset distance can take a value of 0 to 1 mm, for example, 0 mm, 0.5 mm, and 1 mm. Regardless of the first state or the second state, the sample droplet formed on the sample loading part can be brought into contact with the liquid surface in the sample loading part, thereby achieving viscous force between the sample droplet and the liquid surface in the sample loading part, and at the same time The surface tension of the liquid in the sample placement part will also have an effect on the sample droplets. Furthermore, as the sample adding part moves upward, the liquid viscosity and surface tension in the sample placing part, as well as the gravity of the sample droplet itself, fall into the sample placing part, which avoids the occurrence of sample hanging and residue, and improves The accuracy of sample addition can improve the accuracy of sample analysis results when applied to sample analysis.

In an embodiment, before the step S32 in which the sample adding part approaches the sample placing part, the sample adding and mixing method may further include the following step: moving the sample adding part above the sample placing part.

After the sample application part has absorbed the sample, it can form an upper and lower positional relationship with the sample setting part in the vertical direction. Specifically, the sample application part can be moved above the sample setting part. Of course, the sample setting part can also be When the operation is placed in the suspended position, directly move the sample setting part below the sample application part, then the execution of this step can be omitted.

In an embodiment, the step of approaching the sample application part to the sample placement part and the step of placing the sample placement part in a suspended position can be performed at the same time.

If the initial position of the sample loading part is set so that it also needs to move to the vicinity of the suspended position, in order to save the time of part transfer, you can simultaneously control the movement of the sample loading part while performing the operation of placing the sample loading part in the suspended position, so that It moves to above the sample placement part placed in the suspended position.

In one embodiment, the step of placing the sample placement part in the suspended position may be: the transfer part moves the sample placement part from the placement position to the suspended position. Wherein, the distance between the placement position and the detection position is greater than the distance between the suspended position and the detection position, and moving from the suspended position to the detection position can save the moving path of the transfer part and reduce the movement time of the transfer part.

The placement position can be the placement area of the sample placement section, for example, multiple placement sections are placed in the placement section box located on the placement area, or the placement section is placed on the placement area, and the placement area provides a bearing for the placement section. The supporting surface at the bottom of the sample placement section. In the sample adding method in the related art, the sample setting part usually performs the sample adding operation in the placing area, and after the mixing is completed, the sample setting part is moved to the detection position, which takes a lot of time. As shown in FIG. 6A, the sample setting part 2 can be moved from the placement position 1 to the suspended position 3 first, and then from the suspended position 3 to the detection position 4. The placing and moving operation of the sample setting part can be carried out through the transfer part. The transfer part clamps and grabs the sample setting part, and drives the sample setting part to move. After reaching the corresponding position, if there is no supporting surface at the position, such as the above-mentioned suspended position, the transfer part holds and fixes the sample setting part Keep it at the corresponding position. If there is a supporting surface at the position, such as the detection position, the transfer part releases the sample placement part and places it in the corresponding position.

As shown in FIG. 6D, the sample placement part 2 placed on the detection position 4 has completed the sample addition and mixing operations at this time, and the detection operations of the analyte, such as the mixed sample and/or reagent, can be performed.

In one embodiment, referring to FIG. 7, when the position where the sample setting part is in the mixing state is a suspended position, the step S20 of moving the sample setting part to the detection position by the transfer part may include:

S21: The transfer part transfers the sample placement part from the suspended position to the detection position.

S22: The transfer part releases the sample setting part and places the sample setting part at the detection position.

As shown in Figure 8, the transfer part 6 is switched from the state of holding and fixing the sample placing part 2 in the suspended position 3 to the state of driving the sample placing part 2 to move, and moves from the suspended position 3 to the detection position 4, and After reaching the detection position 4, the sample setting part 2 is released, and the movement process is smooth and smooth.

In one embodiment, referring to FIG. 9, the step of moving the sample placement part from the placement position to the suspended position by the transfer part may include:

Step S41: the transfer part clamps the sample placement part located at the placement position;

Step S42: The transfer part transfers the sample setting part to the suspended position.

As shown in FIG. 10, the transfer part 6 clamps the sample part 2 and moves from the placement position 1 to the suspended position 3.

The application also provides a sample analysis method, which includes the sample adding and mixing method of any one of the foregoing embodiments. The sample analysis method can be applied to a coagulation analyzer to perform functional analysis of blood such as fibrinolysis, antifibrinolysis, coagulation, and anticoagulation. The sample may be a blood sample, a blood test reagent, etc., such as blood, blood components, fibrinolysis, antifibrinolysis, coagulation, anticoagulation, and other test reagents, and the blood components may be plasma, blood cells, and the like.

The application also provides a sample adding and mixing device. In one embodiment, as shown in FIG. 11, the sample adding and mixing device may include: a sample adding portion 11, a first sample adding driving portion 12, and a second sample adding portion. The drive unit 13, the transfer unit 14 and the control unit 15. The sample adding part 11 is used for sucking the object to be added and adding the sample into the sample placing part, which can be a sample needle; the first sample adding driving part 12 is used for driving the movement of the sample adding part 11; the first sample adding driving 12 can be It is a power element such as an XYZ three-axis motion platform, a rotary motion platform, and a motor. Of course, it can also be the transfer part 14 shared with the sample setting part; the second sample adding driving part 13 is used to drive the sample adding part 11 to realize the adding The operation of sucking or discharging the substance; the second sample feeding driving part 13 can be a syringe; the working principle of the syringe can be that the plunger in the syringe moves up and down under the drive of the driving motor, which causes the volume of the sealed cavity to change to form different vacuum degrees. To achieve the suction and discharge of liquid. The second sample adding driving part 13 is connected to the sample adding part 11 and can be used to drive the sample adding part 11 to realize the suction or discharge operation of the sample or the reagent. The transfer part 14 is provided with a clamping mechanism for clamping and releasing the sample setting part 11 and a mixing mechanism for mixing the sample setting part; the control part 15 is used for controlling the first sample driving part 12 and the second The sample loading drive unit 13 and the transfer unit 14 are controlled, and may include a processor 151 and a memory 152. The memory 152 stores a computer program. When the computer program is executed by the processor 151, the processor 151 can execute the operation as described in any of the above embodiments. Add sample and mix method. The control unit 15 can drive the movement of the transfer unit 14 through the transfer unit drive unit; the transfer unit drive unit can be an XYZ three-axis motion platform, an air cylinder, a motor, and the like.

Those skilled in the art can understand that the above-mentioned sample adding and mixing method can be made into a control chip or a memory chip and then applied to various types of analysis and detection/analysis instruments, thereby expanding its application scope and application scenarios, and also improving The convenience of its application.

In one embodiment, referring to FIG. 12, the sample adding and mixing device may further include: a sample placing part 16 for placing the object to be added.

It should be pointed out that the components of the above-mentioned sample adding and mixing device can cooperate with each other to realize the aforementioned sample adding and mixing method.

Referring to FIG. 13, the present application also provides a non-transitory computer-readable storage medium that stores a computer program 21. When the computer program 21 is executed by the processor, the processor executes the sample mixing and mixing as described in any of the above-mentioned embodiments. Even method.

A person of ordinary skill in the art can understand that all or part of the processes in the above-mentioned embodiment methods can be implemented by instructing relevant hardware through a computer program. The computer program 21 can be stored in a non-volatile computer readable storage medium. Here, when the program is executed, it may include the procedures of the above-mentioned method embodiments. Wherein, any reference to memory, storage, database, or other media used in the embodiments provided in this application may include non-volatile and/or volatile memory. Non-volatile memory may include read only memory (ROM), programmable ROM (PROM), electrically programmable ROM (EPROM), electrically erasable programmable ROM (EEPROM), or flash memory. Volatile memory may include random access memory (RAM) or external cache memory. As an illustration and not a limitation, RAM is available in many forms, such as static RAM (SRAM), dynamic RAM (DRAM), synchronous DRAM (SDRAM), double data rate SDRAM (DDRSDRAM), enhanced SDRAM (ESDRAM), synchronous chain Channel (Synchlink) DRAM (SLDRAM), memory bus (Rambus) direct RAM (RDRAM), direct memory bus dynamic RAM (DRDRAM), and memory bus dynamic RAM (RDRAM), etc.

The application also provides a sample analysis device, which includes the sample adding and mixing device of any one of the above embodiments. The sample analysis device may be a coagulation analyzer, which is used to perform functional analysis of blood such as fibrinolysis, antifibrinolysis, coagulation, and anticoagulation. The sample may be a blood sample, a blood test reagent, etc., such as blood, blood components, fibrinolysis, antifibrinolysis, coagulation, anticoagulation, and other test reagents, and the blood components may be plasma, blood cells, and the like.

The above are only preferred specific implementations of this application, but the protection scope of this application is not limited to this. Anyone familiar with the technical field within the technical scope disclosed in this application, according to the technical solution of this application and its The equivalent replacement or change of the inventive concept shall be covered by the protection scope of this application. In addition, although some specific terms are used in this specification, these terms are only for convenience of description and do not constitute any limitation to this application.

Claims

A method for adding and mixing samples, including:

Place the sample setting part in a suspended position;

The sample adding part adds an object to be added into the sample placing part;

Before the sample setting part reaches the detection position, a mixing operation is performed on the sample setting part through the transfer part, so that the additives in the sample setting part are mixed uniformly to obtain the test object.
The sample adding and mixing method according to claim 1, wherein the position where the sample setting part is in the mixing state is the suspended position, or the sample setting part moves from the suspended position to the detection position Any position that can be passed during the process;

The detection position is a position where the sample setting part is placed and the test object in the sample setting part is detected.
The sample adding and mixing method according to claim 1, wherein after the step of adding the sample to the sample placing portion by the sample adding portion, the method further comprises:

The transfer part moves the sample placement part to a detection position;

Wherein, the detection position is a position where the sample setting part is placed and the test object in the sample setting part is detected.
The sample adding and mixing method according to claim 3, wherein when the position where the sample setting part is in the mixing state is the suspended position, the transfer part moves the sample setting part The steps to the detection position include:

The transfer part transfers the sample placement part from the suspended position to the detection position;

The transfer part releases the sample setting part, and places the sample setting part on the detection position.
The sample adding and mixing method according to claim 1, wherein when in the suspended position, the sample placement part is in a suspended state under the action of the transfer part.
The sample adding and mixing method according to claim 5, further comprising:

Detecting whether the sample placement part is placed on the transfer part;

When the sample setting part is placed on the transfer part, acquiring the current position of the sample setting part;

According to the current position of the sample setting part, the state of the sample setting part is controlled by the transfer part.
The sample adding and mixing method according to claim 5, wherein the state of the sample setting part is one of the following states: the moving state, the mixing state, the suspended state, It is in the state of the moving state and the mixing state at the same time, and in the state of the mixing state and the suspended state at the same time.
The sample adding and mixing method according to claim 1, wherein the object to be added is a sample and/or a reagent;

The step of adding an object to be added by the sample adding part to the sample placing part includes:

The sample adding part and the sample placing part are close to each other;

The sample adding part adds the object to be added to the sample placing part at the sample adding position;

After the sample application is completed, the sample application part and the sample placement part are far away from each other.
The sample adding and mixing method according to claim 8, wherein the step of bringing the sample adding part and the sample placing part close to each other is performed simultaneously with the step of placing the sample placing part in a suspended position.
The sample adding and mixing method according to claim 8, wherein after the sample adding part at the sample adding position adds the object to be added to the sample placing part, the liquid level in the sample placing part is The sample adding parts are in the first state or the second state;

The first state is that the liquid contact position of the sample application part and the liquid surface in the sample placement part are at the same height level and are in contact with each other; the second state is that the liquid contact position of the sample application part is arranged at intervals It is above the liquid surface in the sample placing part and the distance from the liquid surface in the sample placing part is less than a predetermined distance.
The sample adding and mixing method according to claim 1, wherein the step of placing the sample setting part on a suspended position comprises:

The transfer part moves the sample placement part from the placement position to the suspended position;

Wherein, the distance between the placement position and the detection position is greater than the distance between the suspended position and the detection position.
The sample adding and mixing method according to claim 11, wherein the suspended position is set such that the distance between the placement position and the detection position, the distance between the suspended position and the detection position, and The sum of the distances between the placement position and the suspended position is the shortest.
The sample adding and mixing method according to claim 11, wherein the step of the transferring part to move the sample placing part from the placing position to the suspended position comprises:

The transfer part clamps the sample placement part located at the placement position;

The transfer part transfers the sample placement part to the suspended position.
The sample adding and mixing method according to claim 13, wherein the transfer part is at least one of a manipulator, a finger cylinder, and a clamping jaw.
The sample adding and mixing method according to claim 11, wherein the suspended position is located above the placement position, above the detection position, or between the placement position and the detection position;

The placement position is the position where the sample placement part is placed before being placed in the suspended position.
The sample adding and mixing method according to claim 1, wherein the step of performing a mixing operation on the sample setting part by a transfer part is:

The transfer part applies a driving force to the clamped sample setting part that enables the sample setting part to perform a mixing action;

The driving force includes at least one of rotation force, swing force, and vibration force.
A sample adding and mixing device, wherein the sample adding and mixing device comprises:

The sample adding part is used for adding objects to be added into the sample placing part when the sample placing part is in the suspended position;

The transfer part is used to move the sample setting part from the placing position to the suspended position, and perform a mixing operation on the sample setting part, so that the additives in the sample setting part are mixed uniformly to obtain the The object to be tested, and move the sample placement part to the detection position to detect the object to be tested;

Wherein, the distance between the placement position and the detection position is greater than the distance between the suspended position and the detection position.
The sample adding and mixing device according to claim 17, further comprising:

The sample setting part is used to place the object to be added.
The sample adding and mixing device according to claim 17, further comprising:

The first sample adding driving part is used to drive the sample adding part to move along a preset trajectory;

The second sample adding driving part is used to drive the sample adding part to realize the suction or adding operation of the object to be added.

The control unit is respectively connected with the first sample adding drive unit, the second sample adding drive unit, and the transfer unit, and is used for controlling the first sample adding drive unit and the second sample adding drive unit. Control with the transfer part.
A non-transitory computer-readable storage medium, wherein the non-transitory computer-readable storage medium stores a computer program, and when the computer program is executed by a processor, the processor executes the following steps:

Place the sample placement part in a suspended position through the transplanting part;

The sample adding part adds an object to be added into the sample placing part;

Before the sample setting part reaches the detection position, a mixing operation is performed on the sample setting part through the transfer part, so that the additives in the sample setting part are mixed uniformly to obtain the test object.