WO2021091434A8 - Structure de génie génétique pour stimuler l'angiogenèse - Google Patents

Structure de génie génétique pour stimuler l'angiogenèse Download PDF

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Publication number
WO2021091434A8
WO2021091434A8 PCT/RU2021/050002 RU2021050002W WO2021091434A8 WO 2021091434 A8 WO2021091434 A8 WO 2021091434A8 RU 2021050002 W RU2021050002 W RU 2021050002W WO 2021091434 A8 WO2021091434 A8 WO 2021091434A8
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WIPO (PCT)
Prior art keywords
gene
genetic engineering
angiogenesis
blood supply
growth factor
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PCT/RU2021/050002
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English (en)
Russian (ru)
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WO2021091434A9 (fr
WO2021091434A3 (fr
WO2021091434A2 (fr
Inventor
Екатерина Александровна СЛОБОДКИНА
Максим Николаевич КАРАГЯУР
Вадим Юрьевич Балабаньян
Елена Викторовна ПАРФЕНОВА
Павел Игоревич МАКАРЕВИЧ
Жанна Алексеевна АКОПЯН
Всеволод Арсеньевич ТКАЧУК
Мария Александровна БОЛДЫРЕВА
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Общество С Ограниченной Ответственностью "Генная И Клеточная Терапия"
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Publication of WO2021091434A2 publication Critical patent/WO2021091434A2/fr
Publication of WO2021091434A8 publication Critical patent/WO2021091434A8/fr
Publication of WO2021091434A9 publication Critical patent/WO2021091434A9/fr
Publication of WO2021091434A3 publication Critical patent/WO2021091434A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/515—Angiogenesic factors; Angiogenin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/4753—Hepatocyte growth factor; Scatter factor; Tumor cytotoxic factor II
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biotechnology (AREA)
  • Public Health (AREA)
  • Toxicology (AREA)
  • Vascular Medicine (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Heart & Thoracic Surgery (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cardiology (AREA)
  • Epidemiology (AREA)
  • Wood Science & Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention se rapporte au domaine des biotechnologies, concerne plus précisément des structures de génie génétique pour stimuler l'angiogenèse, et peut être utilisée en médecine afin de traiter des maladies liées à un trouble de l'apport en sang des tissus. L'invention concerne une structure bicistronique plasmide porteuse du gène hHGF de facteur de croissance d'hépatites humain et du gène hVEGF165 de facteur de croissance endothéliale des vaisseaux avec deux promoteurs distincts pour chacun des gènes: promoteur de citomégalovirus pCMV pour le gène HGF et promoteur de gène de β-actine du poussin pCAG pour le gène VEGF165. Cette structure bicistronique peut être utilisée en qualité d'agent médicamenteux afin d'assurer la stimulation de l'angiogenèse, la croissance et le remodelage de vaisseaux, ainsi que la restauration de l'alimentation en sang dans des tissus ischémiques. Cette invention permet d'atteindre des concentrations optimales et une corrélation des facteurs angiogènes de croissance (FAC).
PCT/RU2021/050002 2019-11-06 2021-01-05 Structure de génie génétique pour stimuler l'angiogenèse WO2021091434A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
RU2019135603A RU2737487C1 (ru) 2019-11-06 2019-11-06 Генно-инженерная конструкция для стимуляции ангиогенеза
RU2019135603 2019-11-06

Publications (4)

Publication Number Publication Date
WO2021091434A2 WO2021091434A2 (fr) 2021-05-14
WO2021091434A8 true WO2021091434A8 (fr) 2021-06-10
WO2021091434A9 WO2021091434A9 (fr) 2021-07-08
WO2021091434A3 WO2021091434A3 (fr) 2021-09-23

Family

ID=73792491

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/RU2021/050002 WO2021091434A2 (fr) 2019-11-06 2021-01-05 Structure de génie génétique pour stimuler l'angiogenèse

Country Status (2)

Country Link
RU (1) RU2737487C1 (fr)
WO (1) WO2021091434A2 (fr)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001291019A1 (en) * 2000-09-15 2002-03-26 Genvec, Inc. Method of modulating neovascularization
CN1204247C (zh) * 2001-06-15 2005-06-01 中国人民解放军军事医学科学院百环生物医学研究中心 一种重组腺病毒及其在心肌缺血治疗中的应用
RU2449799C2 (ru) * 2009-10-30 2012-05-10 Федеральное Государственное Учреждение "Российский кардиологический научно-производственный комплекс Федерального агентства по высокотехнологичной медицинской помощи" Средство для осуществления терапевтического ангиогенеза и способ его осуществления
CN102242148A (zh) * 2010-10-09 2011-11-16 苏州大学 重组载体以及转基因骨髓基质细胞修饰的丝素膜及其应用

Also Published As

Publication number Publication date
WO2021091434A9 (fr) 2021-07-08
RU2737487C1 (ru) 2020-12-01
WO2021091434A3 (fr) 2021-09-23
WO2021091434A2 (fr) 2021-05-14

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