WO2021054818A1 - Composition pour éliminer une infestation par des organismes nuisibles - Google Patents

Composition pour éliminer une infestation par des organismes nuisibles Download PDF

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Publication number
WO2021054818A1
WO2021054818A1 PCT/MY2020/050086 MY2020050086W WO2021054818A1 WO 2021054818 A1 WO2021054818 A1 WO 2021054818A1 MY 2020050086 W MY2020050086 W MY 2020050086W WO 2021054818 A1 WO2021054818 A1 WO 2021054818A1
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WO
WIPO (PCT)
Prior art keywords
mycoinsecticide
composition
propagules
bagworm
submerged
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PCT/MY2020/050086
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English (en)
Inventor
Norhayu ASIB
Muhammad Nurul Yaqin SYARIF
Dzolkhifli OMAR
Siti Nursyazwina MD ZAMRI
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Universiti Putra Malaysia
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Application filed by Universiti Putra Malaysia filed Critical Universiti Putra Malaysia
Publication of WO2021054818A1 publication Critical patent/WO2021054818A1/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/79—Paecilomyces

Definitions

  • the present invention relates to a pest control composition comprising entomopathogenic fungi, and a method of using such composition for suppressing plant damaging pests in agricultural environments.
  • Pests such as insects, arachnids and nematodes are a major problem for the agriculture industry, often significantly limiting crop productivity.
  • Psychidae or commonly known as bagworms belongs to the family of Lepidoptera consisting of at least 1,350 species around the world. Bagworms have a detrimental impact on oil palm plantations of which the impact consequently manifests into poor harvest yield of palm oil and huge economic loss to the country.
  • Metisa plana Walker has become one of the important and dominant oil palm pests in Malaysia and Indonesia to the extent of being declared as the most severe leaf defoliators in oil palm plantations in Peninsular Malaysia (Norman, K., G.S. Robinson and M.W. Basri, 1994.
  • Biopesticides have been developed for use as a safer substitute, or alternatively as a supplement to chemical insecticides.
  • Biopesticides are essentially living organisms (for example, bacteria and fungi) that interfere with the life cycles of insects by maiming or killing the insects.
  • Entomopathogenic fungi are one of the biological agents used as biopesticides due to their epizootic nature to insects.
  • Examples of biopesticides include the entomopathogenic fungus Isaria fumosorosea , which has shown huge promise as a biological pesticide. Isaria fumosorosea has been reported to infect a wide range of host including diamondback moth (. Plutella xyllostella ), Russian wheat aphid (.
  • CN104642392A resembling the latter patent document discloses an Isaria fumosorosea-based wettable powder formulation having pure Isaria fumosorosea conidia powder as the active component.
  • a Chinese Patent Document No. CN103749545A discloses a wettable powder formulation comprising a combination of entomopathogenic fungus Isaria fumosorosea conidia and soil-borne Bacillus thuringiensis strains.
  • the above-mentioned patent documents present a drawback as they utilize a conventional dispersion of biopesticides using aerial conidia, in which harvest of conidia of Isaria fumosorosea in mass production is significantly complex and difficult.
  • a mycoinsecticide composition particularly for suppressing bagworm infestation in crops at oil palm plantations by utilizing entomopathogenic fungal propagules, particularly submerged Isaria fumosorosea propagules, a stable surfactant system and an agriculturally acceptable carrier. Accordingly, a method for suppressing bagworm infestation in crops using the mycoinsecticide composition thereof should be accompanied.
  • This invention provides such a composition and a method of dispersing the composition therefrom.
  • One aspect of the invention is to provide a degradable mycoinsecticide composition for suppressing bagworm infestation in a plant.
  • Another aspect of the invention is to provide a method of suppressing bagworm infestation in a plant by treating the plant with a degradable mycoinsecticide composition.
  • At least one of the preceding objects is met, in whole or in part, in which the embodiment of the invention describes a degradable mycoinsecticide composition for suppressing bagworm infestation in a plant comprising a fungal insecticide having submerged propagules derived from a strain of Isaria fumosorosea , a sulfonate surfactant and an agriculturally acceptable carrier.
  • the fungal insecticide may have an insecticidally effective amount of submerged propagules ranging from 10 6 to 10 8 cfu/mL of the mycoinsecticide composition.
  • the sulfonate surfactants are selected from sodium lignosulfonate, sodium xylenesulfonate, sodium cumenesulfonate or a combination thereof.
  • the total amount of sulfonate surfactant mixture may be ranging from 15% by weight to 30% by weight of the mycoinsecticide composition.
  • the agriculturally acceptable carrier may include a bentonite clay in an amount ranging from 50% by weight to 65% by weight of the mycoinsecticide composition.
  • the mycoinsecticide composition may be in the form of a wettable powder.
  • a preferred embodiment of the invention further describes a method of suppressing bagworm infestation in a plant comprising treating the plant with a degradable mycoinsecticide composition comprising a fungal insecticide, a sulfonate surfactant mixture and an agriculturally acceptable carrier.
  • the fungal insecticide may be derived from a strain of Isaria fumosorosea having an insecticidally effective amount of submerged propagules.
  • the sulfonate surfactants are selected from sodium lignosulfonate, sodium xylenesulfonate, sodium cumenesulfonate or a combination thereof.
  • the agriculturally acceptable carrier may preferably include a bentonite clay.
  • the method of of suppressing bagworm infestation in a plant further comprise a step of diluting the mycoinsecticide composition to water at a volume ratio from 1 :200 to 1 :500.
  • the method of suppressing bagworm infestation in a plant may further comprise a step of diluting the mycoinsecticide composition to water to achieve an insecticidally effective amount of 10 6 to 10 8 cfu/mL of submerged propagules derived from the strain of Isaria fumosorosea..
  • the bagworm species may include Metisa plana , Pteroma pendula and Mahasena corbetti.
  • FIG. 1 depicts a pseudoternary phase diagram for all surfactant system tested.
  • the invention relates to a degradable mycoinsecticide composition for suppressing bagworm infestation in a plant.
  • the composition comprises a fungal insecticide having submerged propagules derived from an entomopathogenic fungus strain of Isaria fumosorosea , a sulfonate surfactant mixture and an agriculturally acceptable carrier.
  • the mycoinsecticide composition is effective in suppressing bagworm infestation in a plant, particularly oil palm.
  • the mycoinsecticide composition also presents the benefit of selectively infecting the target pest, particularly bagworm, without harming beneficial insect pollinators.
  • the mycoinseciticide composition is formed of 10 6 to 10 8 cfu/mL of fungal insecticide.
  • the mycoinseciticide composition is comprised of at least one fungal insecticide from the genus Isaria spp., such as Isaria fumosorosea (also may be referred to in the art as Paecilomyces fumosoroseus and Spicaria fumosorosea).
  • the fungal insecticide described herein comprises submerged propagules of the strain Isaria fumosorosea.
  • the submerged propagules derived from the strain of Isaria fumosorosea comprises blastospore, scloteria and mycelia fragments.
  • the fungal insecticide is obtained via isolation of mycelia of Isaria fumosorosea emerging from mycotized dead bagworms. Subsequently, the fungal insecticide is produced from the mycelia in a liquid culture media or a solid culture media fermentation process known in the art. Fermentation processes is conducted using conventional fermentation processes including shake flask cultivation, aerobic liquid culture techniques and small- scale or large-scale fermentation in industrial or laboratory fermenters; these processes are well known in the art.
  • the submerged propagules of Isaria fumorososea is recovered using conventional techniques such as filtration, centrifugation and mechanical recovery.
  • submerged propagules of Isaria fumosorosea are cheaper and easier to harvest for mass production in comparison to conidia.
  • the mycoinsecticide composition is formed of 15% by weight to 30% by weight of surfactants.
  • the mycoinsecticide composition is comprised of one or more surfactants.
  • the surfactants described herein are water soluble anionic surfactants.
  • the mycoinsecticide composition herein comprises one or more water soluble anionic surfactants.
  • the water soluble anionic surfactants described herein are sulfonated surfactants.
  • the mycoinsecticide composition comprises a mixture of sulfonated surfactants comprising of sodium lignosulfonate, sodium xylenesulfonate and sodium cumenesulfonate ranging from 15% by weight to 30% by weight of the mycoinsecticide composition.
  • the mixture of sulfonated surfactants in such percentage ranges suitable for the mycoinsecticide composition described herein enhances dispersibility and suspensibility of the mycoinsecticide composition in aqueous solutions (for example, when the mycoinsecticide composition is diluted with water).
  • the mycoinsecticide composition will be able to be administered efficiently, through a delivery device, without causing adherence of the submerged propagules of Isaria fumosorosea to the inner walls of the delivery device or obstructing the delivery components of the delivery device.
  • the mixture of sulfonated surfactants in the mycoinsecticide composition may have minimal, if not at all any detrimental effects, on the viability and stability of the fungal insecticide.
  • the mycoinsecticide composition is formed of 50% by weight to 65% by weight of a carrier.
  • the mycoinsecticide composition is comprised at least one solid carrier.
  • the solid carrier described herein is bentonite clay.
  • the bentonite clay makes up the total amount of mycoinsecticide composition.
  • the bentonite clay allows the fungal insecticide to remain efficacious, thus allowing the fungal insecticide to be delivered to the body of the pest, particularly delivery of submerged propagules of Isaria fumosorosea onto the bag casing of bagworms, with an effective degree of lethality, enabling the mycoinsecticide composition to be re-dispersed and dispensed into a delivery container and preventing sedimentation of the submerged propagules of Isaria fumosorosea.
  • the incorporation of bentonite clay enables the mycoinsecticide composition be formulated as a powder.
  • the mycoinsecticide composition is formulated in the form of a wettable powder.
  • the invention also relates to a method for suppressing bagworm infestation in a plant comprising treating the crops with a degradable mycoinsecticide composition comprising a fungal insecticide, a sulfonate surfactant mixture and an agriculturally acceptable carrier.
  • the fungal insecticide is derived from an entomopathogenic fungus strain having an insecticidally effective amount of submerged propagules of Isaria fumosorosea. More preferably, the fungal insecticide is having an insecticidally effective amount of submerged propagules of Isaria fumosorosea ranging from 10 6 to 10 8 cfu/mL of the mycoinsecticide composition.
  • the sulfonate surfactant mixture comprises sodium lignosulfonate, sodium xylenesulfonate and sodium cumenesulfonate. More preferably, the total amount of surfactant mixture comprising sodium lignosulfonate, sodium xylenesulfonate and sodium cumenesulfonate ranges from 15% by weight to 30% by weight of the mycoinsecticide composition.
  • the agriculturally acceptable carrier is a bentonite clay. More preferably, the total amount of bentonite clay ranges from 50% by weight to 65% by weight of the mycoinsecticide composition.
  • the mycoinsecticide composition described herein may be applied at varying concentrations to perform the method for suppressing bagworm infestation in a plant by treating the crops with the mycoinsecticide composition disclosed herein.
  • the mycoinsecticide composition is diluted with water.
  • the mycoinsecticide composition is diluted with water at a volume ratio from 1:200 to 1:500.
  • the mycoinsecticide composition is diluted with water to achieve an insecticidally effective amount of 10 6 to 10 8 cfu/mL of submerged propagules derived from the strain of Isaria fumosorosea.
  • the method comprises suppressing one or more plant pests.
  • the method for suppressing pest infestation described herein is targeted at harmful Lepidoptera insects.
  • the harmful Lepidoptera insects include Psychidae family consisting bagworm species of Metisa plana , Pteroma pendula and Mahasena corbetti. Of particular, the latter bagworm species are more prominent in oil palm plantations as defoliators.
  • the method of suppressing bagworm infestation includes treating a plant with the aforementioned mycoinsecticide composition comprising contacting a plant pest with the mycoinsecticide composition.
  • the contacting step may be performed by spraying, dusting or coating.
  • the step of treating the plant is preferably conducted by spraying the diluted mycoinsecticide composition at a vicinity of a plant.
  • the mycoinsecticide composition comes into contact with the bag casing of bagworms, subsequently the submerged propagules of Isaria fumosorosea in the mycoinsecticide composition undergo sporulation thereon, followed by enzymatic penetration and fungal proliferation inside the pest’s body and finally emergence of mycelia into conidia. Consequently, destruction of the pest’s tissues and drainage of nutrients by the fungus causes lethality to the pest.
  • Example 1 Mycoinseciticide formulations
  • Isaria fumosorosea mass production was conducted at Forest Research Institute Malaysia (FRIM). Three types of Isaria fumosorosea wettable powder formulations were prepared at Universiti Putra Malaysia (UPM). Five hundred and twenty-two oil palm (Elaies guineensis) trees of five years old at Federal Land Development Authority at Lepar Hilir were randomly selected for the experiment. The experiment consists of twenty-nine oil palm trees and three replications with five treatments and control. The population oiMetisa plana was monitored from pre- and post- census at 3, 7, 15, and 30 days after treatment (DAT).
  • DAT days after treatment
  • the experiment was designed in Randomised Complete Block Design (RCBD) with three replications. Data were subjected to Analysis of variance (ANOVA) using Statistical Analysis System (SAS) version 9.4. the means were compared by the Honestly Significant Difference (HSD) at significance level of 0.05.
  • ANOVA Analysis of variance
  • SAS Statistical Analysis System
  • FIG. 1 shows a point selected from the mutual stable region for all aurfactant system tested.
  • Mutual stable region has been identified by testing every possible surfactant system from the qualified surfactants from previous experiment. Out of the surfactant system tested, the region selected to find the mutual region has the suspensibility more than 70% and good dispersibility.
  • Example 2 Mean population of bagworm following treatments and control
  • results showed that the mean population of bagworm was decreased over days of treatment, as indicated in the table below.
  • the population of bagworm was reduced to below economic threshold level (ETL), ten larvae per frond within thirty days after treatment application. Nonetheless, the mean population of bagworm was the highest in cniadin followed by flub endi amide, FI 3, FI 1 and FI 2.
  • the control plot was shown the decrease in population number from the previous census p-value of 0.0009 and Tukey’s multiple test showed that there was no significant difference between populations of bagworm in each treatment plots.
  • Example 3 Mean population of pollinating insect following treatments and control
  • the mean population of pollinating weevil in the table below shows the number of population of weevil reducing until 7 DAT and recovering on 14 DAT. At 14 DAT, the overall population of weevil was approximately close to the original number of population before the treatment. The mean population of weevil was about 200 weevils per palm exceptforthe cniadin plot, which was recorded only 173.8 weevils on average.

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Abstract

L'invention concerne une composition mycoinsecticide dégradable comprenant un insecticide fongique ayant des propagules submergées dérivées d'une souche d'Isaria fumosorosea, un mélange tensioactif sulfonate et un support acceptable en agriculture approprié pour supprimer une infestation par des organismes nuisibles.
PCT/MY2020/050086 2019-09-19 2020-09-17 Composition pour éliminer une infestation par des organismes nuisibles WO2021054818A1 (fr)

Applications Claiming Priority (2)

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MYPI2019005433A MY201142A (en) 2019-09-19 2019-09-19 Composition for suppressing pest infestation
MYPI2019005433 2019-09-19

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114480410A (zh) * 2022-03-04 2022-05-13 西南大学 一种利用袋蛾丝蛋白提高蚕丝机械性能的转基因方法及其家蚕品种

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008033751A2 (fr) * 2006-09-11 2008-03-20 The United States Of America, As Represented By The Secretary Of Agriculture Compositions d'hydrolysat de kératine et de microbes destinées à des applications de lutte contre les insectes
CN106376600A (zh) * 2016-08-30 2017-02-08 江西天人生态股份有限公司 一种玫烟色拟青霉可湿性粉剂及制备方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008033751A2 (fr) * 2006-09-11 2008-03-20 The United States Of America, As Represented By The Secretary Of Agriculture Compositions d'hydrolysat de kératine et de microbes destinées à des applications de lutte contre les insectes
CN106376600A (zh) * 2016-08-30 2017-02-08 江西天人生态股份有限公司 一种玫烟色拟青霉可湿性粉剂及制备方法

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"CALSOFT® L-60 MSDS", ISSUED BY PILOT CHEMICAL COMPANY, 3 January 2011 (2011-01-03), Retrieved from the Internet <URL:www.stobec.com/DATA/PRODUIT/1866-v-msds83399.pdf> [retrieved on 20111120] *
SYARIF, M. N.Y.: "Formulation and laboratory efficacy of lsaria fumosorosea against bagworms (Metisa plana Walker and Pteroma pendula Joannis) (Lepidoptera: Psychidae", MASTER THESIS, UNIVERSITI PUTRA MALAYSIA, 2017, XP055808279, Retrieved from the Internet <URL:http://psasir.upm.edu.my/id/eprint/76142/1/FP%202018%2066%20-%20IR.pdf> [retrieved on 20201118] *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114480410A (zh) * 2022-03-04 2022-05-13 西南大学 一种利用袋蛾丝蛋白提高蚕丝机械性能的转基因方法及其家蚕品种

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