WO2021045036A1

WO2021045036A1 - Detection device

Info

Publication number: WO2021045036A1
Application number: PCT/JP2020/033019
Authority: WO
Inventors: 新井進
Original assignee: 住友ベークライト株式会社
Priority date: 2019-09-03
Filing date: 2020-09-01
Publication date: 2021-03-11
Also published as: JPWO2021045036A1

Abstract

Provided is a detection device which has a simpler structure and in which a microstructure for detecting an object to be detected is appropriately formed. The detection device (10) comprises: a cylindrical body part (1); and a film (2) that has a microstructure (7) for detecting an object to be detected present in a liquid and is attached to the body part (1) to close a bottom section (5), having one opening (6), of the body part (1), wherein the distance (R2) between the outer peripheries of the film (2) is smaller than the distance (R51) between the outer walls of the bottom section (5) and is larger than the distance (R52) between the inner walls of the bottom section (5).

Description

Detection device

The present invention relates to a detection device for detecting an object to be detected.

A technique for detecting an object to be detected such as a biological substance in a minute space having a very small volume is known. According to Japanese Patent Application Laid-Open No. 2017-72476 (Patent Document 1), a mixed solution of a reagent and deoxyribonucleic acid (DNA) to be detected is divided into innumerable microdroplets, and a polymerase chain reaction (PCR: Polymerase Chain Reaction) is described. ) Amplifies the DNA so that signals such as fluorescence can be detected from the droplets containing the DNA, and the digital PCR technology for detecting and quantifying the DNA by counting the droplets is mentioned. There is. In order to divide the mixture into microdroplets, a detection device with microstructures (eg wells) with microvolumes is needed. Patent Document 1 discloses a microfluidic device (1000) including a flow path of a mixed solution and a microwell array in which a plurality of wells are formed as a detection device. Hereinafter, the reference reference numerals given in parentheses in the background art are those of Patent Document 1.

As illustrated in FIG. 1 of Patent Document 1, the microfluidic device (1000) includes a bottom member (1100), a lid member (1200), a flow path (1300), and one end of the flow path (1300). The flow path inlet (1210) provided in the flow path (1210), the flow path outlet (1220) provided at the other end of the flow path (1300), and the sealing member (1400) forming the side surface of the flow path (1300). It includes a microwell array (m) arranged inside the flow path (1300). A fluid (a mixed solution of a detection target and a reagent) is introduced into the flow path (1300) from the flow path inlet (1210) using a syringe or the like, and discharged from the flow path outlet (1220). It is configured to allow a fluid to flow through (1300).

Japanese Unexamined Patent Publication No. 2017-72476

The detection device (microfluidic device (1000)) disclosed in Patent Document 1 has a complicated structure. In addition, the complicated structure also affects, and it is necessary to use a syringe or the like to introduce a mixed solution of the detection target and the reagent, which complicates the inspection process. Therefore, there is a need for a detection device having a simpler structure and a microstructure for accommodating cells / microorganisms, viruses, microbeads for adsorbing these biological substances, droplets, etc., and a microstructure for flowing liquids. ..

In view of the above background, it is desired to provide a detection device having a simpler configuration and an appropriately formed fine structure for detecting an object to be detected.

As one aspect, in the detection device in view of the above, a tubular main body portion and a microstructure for detecting an object to be detected are formed, and the bottom portion having one opening of the main body portion is closed. The film attached to the main body as described above is provided, and the distance between the outer circumferences of the film is smaller than the distance between the outer walls of the bottom and larger than the distance between the inner walls of the bottom.

For example, when the main body and the film on which the fine structure is formed are integrally formed, it is conceivable to produce the entire detection device by molding or the like. However, in the case of molding, there is a limit in reducing the thickness of the portion corresponding to the film. On the other hand, when the main body and the film are separated as in this configuration, a film can be made by forming a fine structure in a thinner film material. In production, it is necessary to inspect whether the detection device has an appropriate microstructure, but if the film on which the microstructure is formed (the part corresponding to the film) is relatively thin, By transmitting light, it is easy to confirm the fine structure, and the production cost can be suppressed. Further, the film is formed so that the distance between the outer circumferences of the film is smaller than the distance between the outer walls of the bottom and larger than the distance between the inner walls of the bottom. Therefore, the bottom portion is appropriately closed, and it is also suppressed that the bottom portion protrudes from the bottom portion and easily peels off. As described above, according to this configuration, it is possible to provide a detection device in which a fine structure for detecting a detection target is appropriately formed with a simpler configuration.

Here, it is preferable that the distance between the outer circumferences of the film is at least 0.05 mm smaller than the distance between the outer walls of the bottom and at least 0.6 mm larger than the distance between the inner walls of the bottom.

It is preferable that the outer shape of the film has a size that can surely close the bottom even if the positional deviation between the main body and the film is maximized. According to this configuration, even if this misalignment is 0.05 [mm], the bottom can be reliably closed. Tolerances within 0.05 [mm] are sufficiently feasible tolerances, and the detection device can be appropriately configured. Further, it is preferable that the outer shape of the film has a sticking allowance so that the film is securely attached to the bottom portion and liquid leakage or the like does not occur. According to this configuration, even if the above-mentioned misalignment occurs, a sticking allowance of at least 0.25 [mm] is secured around the distance between the inner walls of the bottom. The sticking allowance of 0.25 [mm] is sufficient to secure the mounting strength between the main body and the film, and the detection device can be appropriately configured.

Here, as one aspect, it is preferable that the microstructure is formed on the entire surface of the film.

When the fine structure is formed on the entire surface of the film in this way, a certain number of fine structures are arranged on the detection device without being affected by the positional deviation when the film and the main body are attached, and at the same time. As many microstructures as physically possible can be placed on the detection device.

As another aspect, the film includes a non-formed region in which the microstructure is not formed on the outer peripheral side of the film, and a formed region in which the microstructure is formed inside the non-formed region. It is preferable that the forming region is set in a range larger than the distance between the inner walls of the bottom portion.

The outer peripheral side of the film is attached to the bottom, but if a microstructure is formed at this attachment location, the microstructure may reduce the contact area between the bottom and the film, resulting in lower mounting strength. By providing the non-formed region as in this configuration, it is possible to suppress a decrease in the contact area between the bottom and the film, and it is possible to secure the mounting strength between the bottom and the film. Further, since the formation region is set in a range larger than the distance between the inner walls of the bottom, a sufficient fine structure can be arranged in the detection device even if the position shift occurs between the main body and the film. ..

As another aspect, the film includes a non-formed region in which the microstructure is not formed on the outer peripheral side of the film, and a formed region in which the microstructure is formed inside the non-formed region. It is preferable that the forming region is set in a range smaller than the distance between the inner walls of the bottom portion.

As described above, if a fine structure is formed on the outer peripheral side of the film to be attached to the bottom, the contact area between the bottom and the film may be reduced due to the fine structure, and the attachment strength may be lowered. If the formation region is set to a range smaller than the distance between the inner walls of the bottom as in this configuration, the microstructure will come into contact with the bottom if there is no misalignment between the main body and the film. However, even if the position shift occurs between the main body and the film, the number of microstructures in contact with the bottom can be suppressed. Therefore, the mounting strength between the bottom and the film can be ensured.

Further, in each of the above-described aspects, the main body portion includes a leg portion that protrudes from the bottom portion along the outermost circumference of the main body portion, and the distance between the outer circumferences of the film is greater than the distance between the inner circumferences of the leg portion. It is also preferable that the size is small.

By providing the legs, it is possible to provide a clearance for the legs to protrude between the film attached to the bottom and other objects. Therefore, the possibility that the film comes into contact with other objects can be reduced, and damage to the film can be suppressed.

Further, in each of the above-described aspects, the main body portion is adjacent to the first tubular portion via the first tubular portion arranged on the side of the bottom portion and the stepped portion, and the first tubular portion is provided. It is preferable to provide a second tubular portion having a larger diameter than that of the above.

When the main body is formed without a step, the liquid charged into the main body spreads to the inner wall of the main body and rises on the inner wall, and the opening on the opposite side of the bottom of the main body is used. It may leak out. However, if the step portion is provided, even if the liquid spreads on the inner wall of the first tubular portion and rises on the inner wall, the rise stays at the position of the step portion due to surface tension. As a result, the liquid is prevented from rising up the inner wall of the second tubular portion and leaking to the outside of the main body portion from the opening on the side opposite to the bottom portion.

Further features and advantages of the detection device will be clarified from the following description of the embodiments described with reference to the drawings.

An exploded perspective view showing a configuration example of a detection device Vertical cross-sectional view showing a configuration example of a detection device Top view seen from the bottom side (film side) of the detection device Partially enlarged view of the film showing an example of a well Sectional view of a film showing an example of a well The figure which shows typically the first arrangement example of the well in a film. The figure which shows typically the example of the 2nd arrangement of wells in a film. The figure which shows typically the 3rd arrangement example of the well in a film Longitudinal section showing another configuration example of the detection device Top view seen from the bottom side (leg side) of the main body showing an example of the leg part Top view seen from the bottom side (leg side) of the main body showing another example of the leg Partial magnified view of the film showing other examples of wells Top view seen from the bottom side (film side) of the detection device of another example

Hereinafter, an embodiment of a detection device for detecting a detection object existing in a liquid will be described with reference to the drawings. Here, for example, a detection device 10 (see FIG. 1 and the like) in which a biomarker existing in a biological sample such as blood is used as a detection target will be described as an example. The detection target is, for example, cells / microorganisms, viruses, microbeads that adsorb these biological substances (biomarkers), and the like. In this embodiment, microbeads are illustrated. The microbeads are a plurality of fine beads (about 2 [μm] or less in diameter) covered with a specific antibody. The microbeads can capture the microproteins of the biomarker, and the biomarker can be detected by a fluorescent label that emits a complex of the beads and the protein. Further, in the present embodiment, a plurality of beads (for example, 10,000 to tens of thousands) are arranged on one surface, and a complex is formed with beads (beads that emit light by a fluorescent label) on which a complex is formed. Beads that are not (beads that do not emit light due to fluorescent labeling) are present on one surface. By detecting the beads emitting light on the surface by image processing, the number of biomarkers in the sample can be accurately detected as a digitized numerical value. The detection device 10 of the present embodiment is formed with a fine structure (for example, well 7 (see FIGS. 4, 5, etc.)) so that such beads can be appropriately arranged on the surface.

FIG. 1 is an exploded perspective view showing a configuration example of the detection device 10. As shown in FIG. 1, in the detection device 10, a tubular main body 1 and a microstructure (here, a well 7) for detecting a detection object existing in a liquid are formed, and the main body is formed. It includes a film 2 attached to the main body 1 so as to close the bottom 5 having one opening 6 of 1. A fine structure such as a well 7, which will be described later, is formed on one surface of the film 2. The film 2 is attached to the main body 1 with its microstructure facing the main body 1. The main body 1 includes a first opening 61 and a second opening 62, and the bottom 5 includes a second opening 62. Further, the main body portion 1 is adjacent to the first tubular portion 4 via the first tubular portion 4 arranged on the side of the bottom portion 5 (the side of the second opening 62) and the stepped portion 8, and is the first. A second tubular portion 3 having a diameter larger than that of the tubular portion 4 is provided. The main body 1 and the film 2 are joined by, for example, welding (bonding) using a laser or ultrasonic waves, or bonding with an adhesive (including an adhesive tape).

FIG. 2 is a vertical cross-sectional view showing a configuration example of the detection device 10, and FIG. 3 is a plan view seen from the bottom 5 side (film 2 side) of the detection device 10. As shown in these figures, the outer diameter R2 (distance between outer circumferences) of the film 2 is smaller than the outer diameter R51 (distance between outer walls: outer diameter of the main body 1 at the bottom 5) of the bottom 5, and the inner diameter R52 of the bottom 5 (distance between outer walls 5). It is larger than the distance between inner walls). Specifically, the outer diameter R2 of the film 2 is larger than the outer diameter R51 of the bottom 5 by the size (first clearance C1) considering the tolerance of positioning accuracy when the film 2 is attached to the main body 1. Small is preferable. For example, when the maximum tolerance (first clearance C1) is 0.05 [mm], the outer diameter R2 of the film 2 is preferably at least 0.05 [mm] smaller than the outer diameter R51 of the bottom 5. ..

Further, the outer diameter R2 of the film 2 can secure a sticking allowance when the film 2 is stuck to the main body 1, and also from the joint portion between the bottom 5 and the film 2 when the liquid is put into the main body 1. It is preferable that a width (second clearance C2) that can prevent liquid leakage from the film is secured. For example, when the width required for joining as a sticking allowance is 0.25 [mm], considering the above-mentioned first clearance C1 (0.05 [mm]), the joining width that can prevent liquid leakage from the joining portion ( The second clearance C2) is 0.3 [mm]. Therefore, it is preferable that the outer diameter R2 of the film 2 is at least 0.6 mm larger than the inner diameter R52 of the bottom portion 5. In the present embodiment, for example, the outer diameter R51 of the main body 1 at the bottom 5 is about 8 to 9 [mm], and the inner diameter R52 of the main body 1 at the bottom 5 is about 6 to 7 [mm].

As described above, the main body portion 1 is adjacent to the first tubular portion 4 via the first tubular portion 4 arranged on the side of the bottom portion 5 and the stepped portion 8, and is more than the first tubular portion 4. It is provided with a second tubular portion 3 having a large diameter. In the present embodiment, the main body 1 is a resin part formed by injection molding or the like. Therefore, in consideration of removing the molding die and charging the liquid into the detection device 10, the tubular shape has a smaller diameter in the direction from the first opening 61 to the second opening 62. Therefore, in each of the first tubular portion 4 and the second tubular portion 3, the inner diameters (R4 and R3) of each become smaller in the direction from the first opening 61 to the second opening 62. It has become. However, a step portion 8 is provided between the first tubular portion 4 and the second tubular portion 3, and the second tubular portion 3 is larger than the maximum inner diameter R4 of the first tubular portion 4. The smallest inner diameter R3 is larger.

When the main body 1 is formed without the step 8 being provided, the liquid charged into the main body 1 spreads to the inner wall 1a of the main body 1 and rises from the first opening 61. There is a possibility of leakage to the outside of the main body 1. However, by providing the stepped portion 8, even if the liquid rises on the inner wall 4a of the first tubular portion 4, the rise is retained in the stepped portion 8 due to surface tension. Therefore, it is possible to prevent the liquid from further rising from the inner wall 3a of the second tubular portion 3 and leaking from the first opening 61 to the outside of the main body portion 1.

FIG. 4 is a partially enlarged view of the film 2 showing an example of the well 7 as a microstructure, and FIG. 5 is a cross-sectional view of the film 2 showing an example of the well 7. As shown in FIG. 4, a plurality of wells 7 are regularly arranged in the film 2. As shown in FIG. 5, the well 7 is formed in a hemispherical shape recessed from the first surface 2a, which is one surface of the film 2, toward the second surface 2b, which is the other surface. In the present embodiment, the thickness 7t of the film 2 is about 50 to 300 [μm], the diameter 7w of the first surface 2a of the well 7 is about 1 to 10 [μm], and the depth 7d from the first surface 2a of the well 7. Is about 1 to 10 [μm], and the arrangement interval 7p (pitch) of the wells 7 is about 3 to 15 [μm]. Further, FIG. 5 shows an example in which the beads 20 are arranged in one well 7, but the beads 20 are arranged one by one in all the wells 7. The film 2 is joined to the main body 1 with the first surface 2a on which the wells 7 are formed facing the bottom 5.

FIG. 6 schematically shows an example of the first arrangement of the wells 7 in the film 2. In the form shown in FIG. 6, the well 7 (microstructure) is formed on the entire surface of the film 2 (the entire surface of the first surface 2a). When the wells 7 are formed on the entire surface of the first surface 2a in this way, a certain number of wells 7 can be detected by a device for detecting a certain number of wells 7 without being affected by misalignment at the time of joining the film 2 and the main body 1. In addition to being placed in 10, as many wells 7 as physically possible can be placed in the detection device 10. In this case, since the portion where the well 7 is formed on the first surface 2a of the film 2 and the bottom portion 5 of the main body portion 1 overlap when viewed in the direction orthogonal to the film surface, the bonding strength may decrease. Therefore, a form in which the well 7 does not overlap with the bottom portion 5 (for example, a second arrangement example described later) or a form in which the overlapping range of the well 7 and the bottom portion 5 is smaller than that in the first arrangement example (for example, a second arrangement described later). The second clearance C2 may be made larger than that of the arrangement example). As described above, the main body 1 and the film 2 are joined by, for example, welding (bonding) using a laser or ultrasonic waves, or bonding with an adhesive (including an adhesive tape). Since the film 2 according to the first arrangement example can be welded to the main body 1 by melting the fine structure, it is particularly preferable that the film 2 is welded by using a laser or ultrasonic waves.

FIG. 7 schematically shows a second arrangement example of the well 7 in the film 2, and FIG. 8 schematically shows a third arrangement example of the well 7 in the film 2. In these forms, the film 2 (first surface 2a) has a non-formed region E2 in which the well 7 (microstructure) is not formed on the outer peripheral side of the film 2 and a well 7 (microstructure) inside the non-formed region E2. It is provided with a formation region E1 in which a is formed. In the second arrangement example illustrated in FIG. 7, the formation region E1 is set in a range larger than the inner diameter R52 of the bottom portion 5. In the third arrangement example illustrated in FIG. 8, the formation region E1 is set in a range smaller than the inner diameter R52 of the bottom portion 5.

In the second arrangement example illustrated in FIG. 7, the formation region E1 is formed in a range larger than the inner diameter R52 of the bottom portion 5 by the third clearance C3 or more. Here, it is preferable that the third clearance C3 is equal to or higher than the first clearance C1. With this configuration, a certain number of wells 7 can be arranged in the detection device 10 even if the position shift occurs when the film 2 and the main body 1 are joined. In addition, as many wells 7 as possible can be physically arranged in the detection device 10 in the same manner as in the first arrangement example described above. Although the overlapping region is smaller than that of the first arrangement example described above, also in the second arrangement example, the portion where the well 7 is formed on the first surface 2a of the film 2 and the bottom portion 5 of the main body portion 1 May overlap when viewed in the direction orthogonal to the film surface. Therefore, the second clearance C2 may be made larger than the form in which the well 7 does not come into contact with the bottom portion 5 (for example, a third arrangement example described later). The film 2 according to the second arrangement example is joined by welding using a laser or ultrasonic waves or by bonding with an adhesive. In welding, welding can be promoted by melting the fine structure, although it is partial, as in the film 2 of the first arrangement example.

In the third arrangement example illustrated in FIG. 8, the formation region E1 is formed in a range smaller than the inner diameter R52 of the bottom 5 by the fourth clearance C4 or more. Here, it is preferable that the fourth clearance C4 is equal to or higher than the first clearance C1. With this configuration, even if a positional shift occurs when the film 2 and the main body 1 are joined, the portion where the well 7 is formed on the first surface 2a of the film 2 and the bottom 5 of the main body 1 remain. There is no overlap when viewed in the direction orthogonal to the film surface. The number of wells 7 that can be arranged in the detection device 10 is smaller than that of the first arrangement example and the second arrangement example, but the film 2 and the film 2 are smaller than the first arrangement example and the second arrangement example. The joint strength with the bottom portion 5 can be ensured. The film 2 according to the third arrangement example is joined by welding using a laser or ultrasonic waves or by bonding with an adhesive.

Such a film 2 is formed, for example, by heat imprinting or UV imprinting processing in which a plate having protrusions corresponding to the wells 7 is brought into contact with the film material to form the wells 7, or photoresist processing. Can be done. As described above with reference to FIG. 5, the thickness of the film 2 is about 200 [μm]. When a portion corresponding to the film 2 is formed by molding or the like, the thickness thereof is 500 [μm] or more. For example, the product inspection of the detection device 10 is performed by irradiating the film 2 with light to transmit it and confirming the shape and the number of the wells 7. When the thickness of the film 2 is thin, light is transmitted, but when the thickness is increased, the light transmission is impaired and inspection cannot be performed, or it is necessary to satisfy the inspection equipment or lengthen the inspection process. As a result, the cost may increase. However, if the wells 7 are formed by heat imprinting on a thin film material, the cost can be reduced.

By the way, as illustrated in FIG. 9, as another embodiment, the main body 1 may include legs 9 protruding from the bottom 5 along the outermost circumference of the main body 1. As shown in FIG. 9, the outer diameter R51 of the bottom portion 5 excluding the leg portion 9 is substantially the same as the inner diameter R9 (inner distance) of the leg portion 9, and in this case, the outer diameter R51 of the film 2 is the leg. It is preferable that the inner diameter of the portion 9 is smaller than the inner diameter R9. Therefore, with respect to the configuration based on the "outer diameter R51 (distance between outer walls) of the bottom portion 5" in the various forms described above as the form without the leg portion 9, the "leg portion" is provided in the form having the leg portion 9. The configuration can be based on the inner diameter R9 (inner distance) of 9.

The leg portion 9 is not limited to the form formed over the entire circumference of the bottom portion 5 as illustrated in FIG. 10, but is partially formed along the circumferential direction of the bottom portion 5 as illustrated in FIG. It may be in the form formed in. When the legs 9 are partially formed, it is preferable that the inner diameter R9 of the legs 9 is the inner diameter of a virtual circle passing through the inner surface of each of the partially formed legs 9 as shown in FIG. ..

By providing the legs 9 on the bottom 5 in this way, it is possible to prevent the film 2 from coming into contact with the desk or the like when the detection device 10 is placed on the desk or the like. When the detection device 10 is used, it is possible to prevent the film 2 from being damaged. Further, even in a box or the like in which the detection device 10 as a product is housed, contact with the film 2 can be suppressed, and deterioration of the product can be suppressed.

[Other Embodiments]
Hereinafter, other embodiments will be described. The configurations of the respective embodiments described below are not limited to those applied independently, and can be applied in combination with the configurations of other embodiments as long as there is no contradiction.

(1) In the above, the well 7 has been illustrated and described as a microstructure. However, the microstructure is not limited to the well 7, and may be a trench 7B as illustrated in FIG. Further, it may be a flow path to which such a trench 7B is connected.

(2) In the above, the form in which the main body 1 is formed in a cylindrical shape has been illustrated and described. However, as long as the main body 1 has a tubular shape, the cross-sectional shape is not limited to a circular shape, and may be an elliptical shape, a rectangular annular shape, or a polygonal (for example, hexagonal, octagonal, dodecagonal, etc.) annular shape. Good (see FIG. 13). When the shape of the main body 1 (bottom 5) is not circular, the outer diameter R51 and the inner diameter R52 of the bottom 5 are not uniquely determined. However, the plane shape of the bottom portion 5 and the plane diameter shape of the film 2 are similar figures including the case where the main body portion 1 has a cylindrical shape. Therefore, regarding the relationship between the outer diameter R51 of the bottom portion 5 and the outer diameter R2 of the film 2 based on the inner diameter R52 (first clearance C1 and second clearance C2), the clearance between two similar figures may be used. it can.

For example, a film 2 corresponding to a similar shape is based on a line segment (for example, a major axis in the case of an elliptical shape or a diagonal line of a polygon) connecting one point (P1) of the outer shape of the bottom portion 5 and another point (P2). Clearance can be defined on a line segment (L) (for example, major axis, diagonal line, etc.) connecting one point (P1) and another point (P2). Therefore, the above-mentioned "outer diameter (distance between outer walls, distance between outer walls)" and "inner diameter (distance between inner walls, distance between inner walls)" also include meanings such as "major diameter" and "diagonal line".

In the example shown in FIG. 13, the reference numerals “R51a” and “R51b” correspond to the “distance between outer walls” corresponding to the outer diameter R51 of the main body portion 1, and the reference numerals “R52a” and “R52b” correspond to the main body portion 1. Corresponds to the "inner wall distance" corresponding to the inner diameter R52, and the symbols "R2a" and "R2b" correspond to the "outer circumference distance" corresponding to the outer diameter R2 of the film 2. Although not shown, the same applies to the “inner distance” when the legs 9 are provided. The distance between the outer walls, the distance between the outer walls, the distance between the inner walls, and the distance between the inner walls pass through a reference point (Q) such as the center of gravity or the center of the target plane figure (shape of the main body 1 or the like in a plan view). Suitable. The same applies to the microstructure-forming region E1 such as the well 7.

(3) In the above, the main body portion 1 is adjacent to the first tubular portion 4 via the first tubular portion 4 arranged on the side of the bottom portion 5 and the stepped portion 8, and the first tubular portion 4 The embodiment including the second tubular portion 3 having a larger diameter than the above has been described as an example. However, the main body 1 may be formed in a continuous tubular shape without providing the stepped portion 8. For example, when it is assumed that the amount of liquid injected into the main body 1 is sufficiently smaller than the volume of the main body 1, the liquid flows out from the first opening 61 due to surface tension. Since the possibility is low, it is not necessary to provide the step portion 8.

1: Main body part 2: Film 3: Second tubular part 4: First tubular part 5: Bottom part 6: Opening 7: Well (microstructure)
7B: Trench (microstructure)
8: Step 9: Leg 10: Detection device E1: Formed region E2: Non-formed region R2: Outer diameter of film (distance between outer circumferences)
R3: Inner diameter of the second tubular part R4: Inner diameter of the first tubular part R51: Outer diameter of the bottom (distance between outer walls)
R52: Inner diameter of bottom (distance between inner walls)
R9: Inner diameter of the leg (distance between the inside)

Claims

Cylindrical body and
A microstructure for detecting an object to be detected is formed, and a film attached to the main body so as to close the bottom having one opening of the main body is provided.
A detection device in which the distance between the outer circumferences of the film is smaller than the distance between the outer walls of the bottom and larger than the distance between the inner walls of the bottom.
The detection device according to claim 1, wherein the distance between the outer circumferences of the film is at least 0.05 mm smaller than the distance between the outer walls of the bottom and at least 0.6 mm larger than the distance between the inner walls of the bottom. ..
The detection device according to claim 1 or 2, wherein the microstructure is formed on the entire surface of the film.
The film includes a non-formed region in which the microstructure is not formed on the outer peripheral side of the film, and a formed region in which the microstructure is formed inside the non-formed region.
The detection device according to claim 1 or 2, wherein the formation region is set in a range larger than the distance between the inner walls of the bottom portion.
The film includes a non-formed region in which the microstructure is not formed on the outer peripheral side of the film, and a formed region in which the microstructure is formed inside the non-formed region.
The detection device according to claim 1 or 2, wherein the formation region is set in a range smaller than the distance between the inner walls of the bottom portion.
Claims 1 to 5, wherein the main body includes legs protruding from the bottom along the outermost circumference of the main body, and the distance between the outer circumferences of the film is smaller than the distance between the inner sides of the legs. The detection device according to any one item.
The main body portion is adjacent to the first tubular portion via a stepped portion and a first tubular portion arranged on the side of the bottom portion, and has a second tubular shape having a diameter larger than that of the first tubular portion. The detection device according to any one of claims 1 to 6, further comprising a unit.