WO2021032128A1 - 氟取代的2-氨基噻唑-5-芳香族甲酰胺的用途 - Google Patents

氟取代的2-氨基噻唑-5-芳香族甲酰胺的用途 Download PDF

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WO2021032128A1
WO2021032128A1 PCT/CN2020/110061 CN2020110061W WO2021032128A1 WO 2021032128 A1 WO2021032128 A1 WO 2021032128A1 CN 2020110061 W CN2020110061 W CN 2020110061W WO 2021032128 A1 WO2021032128 A1 WO 2021032128A1
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compound
disease
pharmaceutically acceptable
formula
cancer
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PCT/CN2020/110061
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English (en)
French (fr)
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皮士卿
徐燕
杨代鸿
周志刚
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湖南华纳大药厂股份有限公司
湖南新医科技发展有限公司
湖南华纳大药厂科技开发有限公司
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Priority to EP20853834.8A priority Critical patent/EP4019020A4/en
Priority to CN202080030953.6A priority patent/CN113727713A/zh
Priority to US17/636,476 priority patent/US20220249480A1/en
Priority to CA3148436A priority patent/CA3148436A1/en
Publication of WO2021032128A1 publication Critical patent/WO2021032128A1/zh

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/506—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim not condensed and containing further heterocyclic rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A61P11/06—Antiasthmatics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61P17/00—Drugs for dermatological disorders
    • A61P17/06—Antipsoriatics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A61P19/10—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease for osteoporosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/04—Anorexiants; Antiobesity agents
    • A—HUMAN NECESSITIES
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/12—Drugs for disorders of the metabolism for electrolyte homeostasis
    • A61P3/14—Drugs for disorders of the metabolism for electrolyte homeostasis for calcium homeostasis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/02—Antineoplastic agents specific for leukemia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • A—HUMAN NECESSITIES
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    • A—HUMAN NECESSITIES
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    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
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    • A—HUMAN NECESSITIES
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    • A—HUMAN NECESSITIES
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis

Definitions

  • This application belongs to the field of medicinal chemistry, and specifically relates to a fluorine-substituted 2-aminothiazole-5-aromatic carboxamide for the treatment and prevention of diseases and disorders or its pharmaceutical composition in diseases related to tyrosine kinases (such as immunity Diseases, tumors, neurological diseases and other diseases).
  • diseases related to tyrosine kinases such as immunity Diseases, tumors, neurological diseases and other diseases.
  • Protein kinases are a class of enzymes that catalyze the phosphorylation of proteins, thereby changing their substrate activity or the ability to bind to other proteins.
  • the kinase signaling pathway is the most common form of reversible post-translational modification and controls many aspects of cell function.
  • Abnormal activation of protein kinases is the main sign of malignant tumors, including changes in cell proliferation, survival, movement and metabolism, as well as diseases such as vascular proliferation and evasion of anti-tumor immune responses.
  • Bcr-Abl tyrosine kinase in the pathogenesis of chronic myeloid leukemia (CML), and Bcr-Abl inhibitors have paved the way for the development of new targeted therapy small molecule drugs.
  • Imatinib, Bosutinib, Dasatinib, Nilotinib, Radotinib, and Ponatinib have been Approved for the treatment of chronic myelogenous leukemia eosinophilia syndrome, myelodysplastic/myeloproliferative tumor, chronic myelogenous leukemia, acute lymphocytic leukemia/lymphocytic lymphoma.
  • TKIs poorly selective tyrosinase inhibitors
  • BTK tyrosine kinase
  • Bcr-Abl inhibitors for tumor treatment, and can fundamentally improve the average survival rate of patients.
  • new gene mutations and drug resistance forms will also appear in clinical practice, so further development of new inhibitors is needed.
  • Large doses of drugs can cause mutations in the target protein, leading to loss of activity.
  • the application provides a compound of formula I or a pharmaceutically acceptable salt, ester, solvate, prodrug, active metabolite, crystal, stereoisomer, tautomer or geometric isomer thereof or A pharmaceutical composition comprising the compound of formula I or a pharmaceutically acceptable salt, ester, solvate, prodrug, active metabolite, crystal, stereoisomer, tautomer or geometric isomer thereof
  • a pharmaceutical composition comprising the compound of formula I or a pharmaceutically acceptable salt, ester, solvate, prodrug, active metabolite, crystal, stereoisomer, tautomer or geometric isomer thereof
  • this application provides a compound of formula I or a pharmaceutically acceptable salt, ester, solvate, prodrug, active metabolite, crystal, stereoisomer, tautomer or geometric isomer thereof, or A pharmaceutical composition comprising the compound of formula I or a pharmaceutically acceptable salt, ester, solvate, prodrug, active metabolite, crystal, stereoisomer, tautomer or geometric isomer thereof Use in preparing drugs for inhibiting tyrosine kinase activity.
  • the present application provides a method for preventing or treating diseases related to tyrosine kinases, the method comprising administering to an individual in need a therapeutically effective amount of a compound of formula I or a pharmaceutically acceptable salt, ester, Solvates, prodrugs, active metabolites, crystals, stereoisomers, tautomers or geometric isomers or compounds comprising the formula I or pharmaceutically acceptable salts, esters, solvates, pro Pharmaceutical compositions of drugs, active metabolites, crystals, stereoisomers, tautomers or geometric isomers.
  • the present application provides a method for inhibiting the activity of tyrosine kinase, the method comprising administering a compound of formula I or a pharmaceutically acceptable salt, ester, solvate, prodrug, Active metabolites, crystals, stereoisomers, tautomers or geometric isomers, or compounds comprising the formula I or pharmaceutically acceptable salts, esters, solvates, prodrugs, active metabolites, Pharmaceutical compositions of crystals, stereoisomers, tautomers or geometric isomers.
  • the method of inhibiting tyrosine kinase activity is performed in vivo or in vitro.
  • the application provides a compound of formula I or a pharmaceutically acceptable salt, ester, solvate, prodrug, active metabolite, crystal, stereoisomer, tautomer or geometric isomer thereof or A pharmaceutical composition comprising the compound of Formula I or a pharmaceutically acceptable salt, ester, solvate, prodrug, active metabolite, crystal, stereoisomer, tautomer or geometric isomer thereof, It is used to inhibit tyrosine kinase activity.
  • this application provides a compound of formula I or a pharmaceutically acceptable salt, ester, solvate, prodrug, active metabolite, crystal, stereoisomer, tautomer or geometric isomer thereof or A pharmaceutical composition comprising the compound of Formula I or a pharmaceutically acceptable salt, ester, solvate, prodrug, active metabolite, crystal, stereoisomer, tautomer or geometric isomer thereof, It is used to prevent or treat diseases related to tyrosine kinases.
  • the pharmaceutical composition further includes a pharmaceutically acceptable carrier, excipient or adjuvant.
  • the medicament further comprises another therapeutic agent for combination therapy.
  • the another therapeutic agent is selected from one or more of the following: cyclophosphamide, ifosfamide, vincristine, daunorubicin, doxorubicin, cytarabine, Mitoxantrone, dacarbazine, Idarubicin, Tretinoin, Prednisone, Dexamethasone, Mercaptopurine, Methotrexate, Paclitaxel, Melphalan, Long-acting Interferon, Venetog, Gram Zoltinib, Erlotinib, Ositinib, Ruxotinib, Afatinib, Erlotinib, Imatinib, Lapatinib, Bevacizumab, Trastuzumab, Li Tuximab, Cetuximab, Bonatumumab, Fludarabine, Gemcitabine, Decitab
  • the diseases associated with tyrosine kinases are diseases, disorders, and conditions that benefit from the inhibition or reduction of tyrosine kinase activity.
  • the tyrosine kinase includes Bcr-Abl tyrosine kinase and BTK tyrosine kinase.
  • the disease is selected from cancer.
  • the cancer is selected from: chronic myelogenous leukemia (CML), gastrointestinal stromal tumor (GIST), small cell lung cancer (SCLC), non-small cell lung cancer (NSCLC), multiple Myeloma, solid tumor, B-cell lymphoma, chronic lymphocytic leukemia (CLL), acute lymphocytic leukemia (ALL), non-Hodgkin’s lymphoma (NHL), small lymphocytic lymphoma (SLL), sleeve Cell lymphoma (MCL), melanoma, mastocytosis, germ cell tumors, acute myeloid leukemia (AML), marginal zone/diffuse large B-cell lymphoma, sarcoma, pancreatic cancer, malignant glioma, head and neck Tumors, macroglobulinemia, follicular center lymphoma, prostate cancer, myelodysplastic syndrome, atherosclerotic myelodysp
  • the cancer is selected from cancers that are resistant to the targets BCR-ABL and c-KIT by chemotherapeutic agents, and cancers that are resistant to imatinib.
  • the diseases, disorders, and conditions are selected from: bone metastases, hypercalcemia and/or osteoporosis; lung fibrosis; cardiovascular diseases or symptoms; mast cell-mediated Inflammatory diseases; HTLV-1-related myelopathy/tropical spasmodic paralysis; complex regional pain syndrome (CRPS); weight loss or fat loss; arterial obstructive diseases; ubiquitination; diseases related to reduced sugar degradation or Symptoms; Fridreich ataxia; Parkinson's disease progression; Transplant rejection; Rheumatoid arthritis; Graft versus host disease; Autoimmune disease; Recurrent immune thrombocytopenic purpura; Pemphigus vulgaris; Systemic erythema Lupus; scleroderma pulmonary fibrosis; and spontaneous urticaria.
  • the cardiovascular disease or symptom is a cardiovascular disease caused by RASopathy, or a congenital heart disease related to Noonan or Noonan syndrome.
  • the mast cell-mediated inflammatory disease is selected from the group consisting of osteoarthritis, asthma, chronic obstructive pulmonary disease, uveitis, aspirin exacerbated respiratory disease (AERD), and Parkinson’s disease.
  • pharmaceutically acceptable salt refers to a salt that retains the biological efficacy of the free acid and free base of the specified compound, and has no adverse biological or other effects.
  • the compounds described herein can have acidic or basic groups, and therefore can react with any of a variety of inorganic or organic bases, as well as inorganic and organic acids, to form pharmaceutically acceptable salts.
  • These salts can be prepared by the following methods: prepared in situ during the final isolation and purification of the compound of the present application, or by separately reacting the free base form of the compound of the present application with a suitable organic or inorganic acid and isolating the salt formed thereby.
  • Examples of pharmaceutically acceptable salts include salts prepared by the reaction between a compound described herein and an inorganic acid or organic acid or inorganic base or organic base. These salts include acetate, acrylate, adipate, alginate, aspartate, benzoate, benzenesulfonate, bisulfate, bisulfite, bromide, butyrate , Butyne-1,4-diolate, camphorate, camphorsulfonate, caprylate, chlorobenzoate, chloride, citrate, cyclopentane propionate, caprate, Gluconate, dihydrogen phosphate, dinitrobenzoate, lauryl sulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate, glycolate, Hemisulfate, heptanoate, hexyne-1,6-dioate, hydroxybenzoate, y-hydroxybutyrate
  • acids such as oxalic acid
  • acids can be used as intermediates in the preparation of salts to obtain the compounds of the application and their pharmaceutically acceptable acid addition salts (See the examples in Berge et al., J. Pharm. Sci. 1977, 66, 1-19).
  • the compounds described herein that may include free acid groups may be reacted with a suitable base (e.g., a hydroxide, carbonate, or bicarbonate of a pharmaceutically acceptable metal cation), reacted with ammonia, or reacted with a pharmaceutical Acceptable organic primary, secondary or tertiary amine reactions.
  • a suitable base e.g., a hydroxide, carbonate, or bicarbonate of a pharmaceutically acceptable metal cation
  • Representative alkali metal or alkaline earth metal salts include lithium salt, sodium salt, potassium salt, calcium salt, magnesium salt, aluminum salt, and the like.
  • Illustrative examples of the base include sodium hydroxide, potassium hydroxide, hydroxyethyltrimethylamine hydroxide, sodium carbonate, IV'(C 1-4 alkyl) 4 and the like.
  • Representative organic amines used to form base addition salts include ethylamine, diethylamine, ethylenediamine, ethanolamine, diethanolamine, piperazine, and the like. It should be understood that the compounds described herein also include quaternary ammonium compounds of any basic nitrogen-containing groups that they may contain. Water-soluble or oil-soluble or dispersible products can be obtained by quaternization reactions. See, for example, Berge et al. above.
  • solvate refers to a solvate formed by combining the compound of the present application and solvent molecules.
  • the solvate is a monohydrate, for example, the solvent is water, and the compound of the present application forms a monohydrate with water.
  • esters of the compounds of the present invention refer to those pharmaceutically acceptable esters that are suitable for use in contact with the tissues of patients without excessive toxicity, irritation, allergic reactions, etc., within the scope of reasonable medical judgment. It should be understood that the esters of compounds of formula I containing carboxyl or hydroxyl groups include esters that are hydrolyzable in vivo, such as pharmaceutically acceptable esters that are hydrolyzed in humans or animals to form parent acids or alcohols.
  • Suitable pharmaceutically acceptable esters of the carboxyl group formed on the carboxyl group (if present) of the compound of the present invention include, for example, alkyl esters (such as C 1-6 or C 1-4 alkyl esters), cycloalkyl esters (such as C 3-12 , C 3-8 or C 3-6 cycloalkyl ester), aryl alkyl ester (such as C 6-12 or C 6-8 aryl alkyl ester) and heteroaryl alkyl ester (such as C 6-12 or C 6-8 heteroaryl alkyl ester) and the like.
  • alkyl esters such as C 1-6 or C 1-4 alkyl esters
  • cycloalkyl esters such as C 3-12 , C 3-8 or C 3-6 cycloalkyl ester
  • aryl alkyl ester such as C 6-12 or C 6-8 aryl alkyl ester
  • heteroaryl alkyl ester such as C 6-12 or C 6-8 heteroaryl alkyl este
  • the in vivo hydrolyzable esters of the compounds of the present invention containing a hydroxyl group include inorganic esters such as phosphoric acid esters, [ ⁇ ]-acyloxyalkyl ethers, and compounds related to the result of hydrolysis and decomposition of esters in vivo to obtain parent hydroxyl groups.
  • Further suitable in vivo hydrolyzable esters include those formed from amino acids. For example, an ester formed by reacting the hydroxyl group of a compound with the carboxylic acid of an amino acid.
  • Further in vivo hydrolyzable esters include phosphoramidates, and also include the compounds of the present invention in which any free hydroxyl groups independently form phosphoryl or phosphite esters.
  • the present invention includes all possible crystal forms or polymorphs of the compound of the present invention, including individual polymorphs or mixtures of multiple polymorphs in any ratio.
  • polymorph means that the compound of the present application exists in different crystal lattice forms.
  • prodrug includes acid derivatives or alcohol derivatives, etc., which are well-known to practitioners in the art.
  • acid derivatives are, for example, esters prepared by the reaction of a parent acid and a suitable alcohol, or amides, or acid anhydrides, or mixed acid anhydrides prepared by the reaction of a parent acid and a substituted or unsubstituted amine;
  • the alcohol derivatives are, for example, selected from Alcohol esters, alcohol alkoxylates, alcohol ethers, carboxylic acids, carboxylic acid esters, phosphates, and mixtures thereof.
  • Simple aliphatic or aromatic esters, amides and anhydrides, and phosphates on hydroxyl groups produced from acidic groups overhanging the compounds of the present invention are specific prodrugs.
  • active metabolite refers to a biologically active derivative of a compound that is formed when the compound is metabolized.
  • Certain compounds of this application may have asymmetric carbon atoms (stereocenters) or double bonds. Therefore, racemates, diastereomers, enantiomers, geometric isomers and individual isomers are all included within the scope of the present application.
  • the compounds of the present application may exist in specific geometric or stereoisomeric forms. This application contemplates all such compounds, including tautomers, cis and trans isomers, (-)- and (+)-enantiomers, (R)- and (S)-enantiomers , Diastereomers, (D)-isomers, (L)-isomers, and their racemic mixtures and other mixtures, such as enantiomers or diastereomer-enriched mixtures, All these are within the scope of this application. Additional asymmetric carbon atoms may be present in substituents such as alkyl groups. All these isomers and their mixtures are included in the scope of this application.
  • optically active (R)- and (S)-isomers and D and L isomers can be prepared by chiral synthesis or chiral reagents or other conventional techniques. If you want to obtain an enantiomer of a compound of this application, it can be prepared by asymmetric synthesis or derivatization with a chiral auxiliary, in which the resulting diastereomeric mixture is separated and the auxiliary group is cleaved to provide pure The desired enantiomer.
  • the molecule when the molecule contains a basic functional group (such as an amino group) or an acidic functional group (such as a carboxyl group), it forms a diastereomeric salt with a suitable optically active acid or base, and then passes through a conventional method known in the art The diastereoisomers are resolved, and then the pure enantiomers are recovered.
  • the separation of enantiomers and diastereomers is usually accomplished through the use of chromatography, which employs a chiral stationary phase and is optionally combined with chemical derivatization (for example, the formation of amino groups from amines). Formate).
  • the present application also includes compounds of the present application that are the same as those described herein, but with one or more atoms replaced by an isotope-labeled atom having an atomic weight or mass number different from those generally found in nature.
  • isotopes that can be incorporated into the compounds of the present application include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorus, sulfur, fluorine, iodine, and chlorine, such as 2 H, 3 H, 11 C, 13 C, 14 C, 13 N, 15 N, 15 O, 17 O, 18 O, 31 P, 32 P, 35 S, 18 F, 123 I, 125 I and 36 Cl, etc.
  • Certain isotope-labeled compounds of the application can be used in the analysis of compound and/or substrate tissue distribution. Tritiated (ie 3 H) and carbon-14 (ie 14 C) isotopes are especially preferred due to their ease of preparation and detectability.
  • Positron emission isotopes, such as 15 O, 13 N, 11 C, and 18 F can be used in positron emission tomography (PET) studies to determine substrate occupancy.
  • PET positron emission tomography
  • the isotope-labeled compounds of the present application can generally be prepared by the following procedures similar to those disclosed in the schemes and/or examples below, by replacing non-isotopically-labeled reagents with isotope-labeled reagents.
  • substitution with heavier isotopes can provide certain therapeutic advantages resulting from higher metabolic stability (for example, increased in vivo half-life or reduced dosage requirements), and therefore in certain situations
  • the following may be preferred, where the deuterium substitution may be partial or complete.
  • Partial deuterium substitution refers to the substitution of at least one hydrogen with at least one deuterium. All compounds in such forms are included within the scope of the present application.
  • subject refers to an individual suffering from a disease, disorder or condition, etc., including mammals and non-mammals.
  • mammals include, but are not limited to, any member of the class Mammals: humans, non-human primates (such as chimpanzees and other apes and monkeys); domestic animals such as cows, horses, sheep, goats, pigs; domestic animals , Such as rabbits, dogs and cats; laboratory animals, including rodents, such as rats, mice and guinea pigs.
  • non-human mammals include, but are not limited to, birds and fish.
  • the mammal is a human.
  • treatment means administering the compound or formulation described in this application to ameliorate or eliminate a disease or one or more symptoms related to the disease, and includes:
  • prevention means administering the compound or formulation described in this application to prevent a disease or one or more symptoms related to the disease, and includes: preventing the occurrence of a disease or disease state in an individual, especially when the individual When susceptible to the disease state, but has not been diagnosed as having the disease state.
  • an effective amount refers to the amount of at least one agent or compound that is sufficient to relieve one or more symptoms of the disease or condition being treated after administration. The result can be a reduction and/or alleviation of signs, symptoms or causes, or any other desired changes in the biological system.
  • the "effective amount” for treatment is the amount of the composition containing the compound disclosed herein that is required to provide significant disease relief clinically. Techniques such as dose escalation tests can be used to determine the effective amount suitable for any individual case.
  • acceptable refers to no long-term harmful effects on the general health of the subject being treated.
  • pharmaceutically acceptable refers to a substance (such as a carrier or diluent) that does not affect the biological activity or properties of the compound of the present application, and is relatively non-toxic, that is, the substance can be administered to an individual without causing adverse biological reactions or Interacts with any components included in the composition in an undesirable manner.
  • composition refers to a biologically active compound optionally mixed with at least one pharmaceutically acceptable chemical ingredient, the pharmaceutically acceptable chemical ingredient including but not limited to a carrier, a stabilizer , Diluent, dispersant, suspending agent, thickener and/or excipient.
  • carrier refers to a relatively non-toxic chemical compound or reagent that helps to introduce the compound into cells or tissues.
  • pharmaceutically acceptable excipients refers to those excipients that have no obvious stimulating effect on the organism and will not damage the biological activity and performance of the active compound.
  • Suitable auxiliary materials are well known to those skilled in the art, such as carbohydrates, waxes, water-soluble and/or water-swellable polymers, hydrophilic or hydrophobic materials, gelatin, oils, solvents, water and the like.
  • the pharmaceutical composition of the application can be prepared by combining the compound of the application with suitable pharmaceutically acceptable excipients, for example, can be formulated into solid, semi-solid, liquid or gaseous preparations, such as tablets, pills, capsules, powders , Granules, lozenges, ointments, syrups, emulsions, suspensions, solutions, suppositories, injections, inhalants, gels, microspheres and aerosols.
  • suitable pharmaceutically acceptable excipients for example, can be formulated into solid, semi-solid, liquid or gaseous preparations, such as tablets, pills, capsules, powders , Granules, lozenges, ointments, syrups, emulsions, suspensions, solutions, suppositories, injections, inhalants, gels, microspheres and aerosols.
  • Drug routes or routes of administration include, but are not limited to, oral, rectal, transmucosal, topical, transdermal, inhalation, parenteral, sublingual, intravaginal, intranasal, intraocular, intraperitoneal, intramuscular, subcutaneous and intravenous administration .
  • the preferred route of administration is oral administration and injection administration.
  • the compound or composition of the present invention can be formulated and used as the following dosage forms: tablets, capsules or elixirs for oral administration; suppositories for rectal administration; sterile solutions and suspensions for injection administration; Transdermal patches and subcutaneous deposits.
  • Injections can be prepared in the following conventional forms: solutions or suspensions, solid dosage forms suitable for making solutions or suspensions before injection, or emulsions. Suitable excipients are, for example, water, saline, glucose, mannitol, lactose, lecithin, albumin, sodium glutamate, cysteine hydrochloride and the like.
  • the pharmaceutical composition for injection may contain a relatively small amount of non-toxic auxiliary substances, such as wetting agents, pH buffering agents and the like. If necessary, absorption enhancers (for example, liposomes) may also be used.
  • Formulations for parenteral administration include aqueous solutions of the active compound in water-soluble form.
  • the suspension of the active compound can be prepared into a suitable oily injection suspension.
  • suitable lipophilic solvents or carriers include fatty oils such as sesame oil, or other organic oils such as soybean oil, grapefruit oil or almond oil, or synthetic fatty acid esters such as ethyl oleate or triglycerides, or liposomes .
  • Aqueous injection suspensions may contain substances that increase the viscosity of the suspension, such as sodium carboxymethyl cellulose, sorbitol, or dextran.
  • the suspension may contain a suitable stabilizer or agent that improves the solubility of the compound, so that a highly concentrated solution can be prepared.
  • the pharmaceutical composition of the present application can be prepared by methods known in the art, such as conventional mixing method, dissolution method, granulation method, sugar-coated pill method, grinding method, emulsification method and freeze-drying method.
  • the pharmaceutical composition of the application is in oral form.
  • the pharmaceutical composition can be formulated by mixing the active compound with pharmaceutically acceptable excipients or excipients well known in the art. These adjuvants or excipients can make the compound of the present application be formulated into tablets, pills, lozenges, sugar coatings, capsules, powders, granules, liquids, syrups, emulsions, gels, slurries, suspensions Etc., for oral administration to patients.
  • the solid pharmaceutical composition suitable for oral administration can be prepared by conventional mixing, filling or tableting methods.
  • an oral composition in solid form can be obtained by the following method: mixing the active compound with solid excipients or excipients, optionally grinding the resulting mixture, adding other suitable excipients or excipients if necessary, and then The mixture is processed into granules to obtain tablets or dragee cores.
  • Suitable auxiliary materials or excipients include, but are not limited to: fillers, binders, diluents, disintegrants, lubricants, glidants, sweeteners or flavoring agents, and the like.
  • a pharmaceutical preparation for oral administration can be obtained by combining the active compound with a solid excipient, optionally grinding the resulting mixture, and processing the granular mixture, if necessary, adding suitable auxiliary agents The tablet or sugar-coated core is then obtained.
  • suitable excipients are, in particular, fillers such as sugars, including lactose, sucrose, mannitol or sorbitol; cellulose preparations such as corn starch, wheat starch, rice starch, potato starch, gelatin, tragacanth, Methyl cellulose, hydroxypropyl methyl cellulose, sodium carboxymethyl cellulose, and/or polyvinylpyrrolidone (PVP).
  • disintegrating agents may be added, such as cross-linked polyvinylpyrrolidone, agar, or alginic acid or alginates such as sodium alginate.
  • the sugar coating core is suitably coated.
  • a concentrated sugar solution may be used, which may optionally contain gum arabic, talc, polyvinylpyrrolidone, carbopol gel, polyethylene glycol and/or titanium dioxide, shellac paint solution and suitable organic solvents or Solvent mixture.
  • dyes or pigments can be added to the tablets or dragee coatings. These formulations can be manufactured using methods well known in the art.
  • the daily dose may be, for example, 0.001-300 mg/kg body weight, such as 0.01-300 mg/kg body weight, or 10-200 mg/kg body weight, in a single dose or divided Dosage form administration.
  • the pharmaceutical composition of the present application can also be suitable for parenteral administration, such as a sterile solution, suspension, emulsion or lyophilized product in a suitable unit dosage form.
  • Suitable excipients such as fillers, buffers or surfactants can be used.
  • This application takes Bcr-Abl and BTK as targets and develops a kinase inhibitor for the prevention or treatment of immune diseases, tumors and neurological diseases.
  • the present application provides a compound of formula I or a pharmaceutically acceptable salt, ester, solvate, prodrug, active metabolite, crystal, stereoisomer, tautomer or geometric isomer thereof or includes
  • the pharmaceutical composition of the compound of formula I or a pharmaceutically acceptable salt, ester, solvate, prodrug, active metabolite, crystal, stereoisomer, tautomer or geometric isomer thereof is being prepared Use in drugs for preventing or treating diseases related to tyrosine kinases,
  • the application provides a compound of formula I or a pharmaceutically acceptable salt, ester, solvate, prodrug, active metabolite, crystal, stereoisomer, tautomer or geometric isomer or A pharmaceutical composition comprising the compound of formula I or a pharmaceutically acceptable salt, ester, solvate, prodrug, active metabolite, crystal, stereoisomer, tautomer or geometric isomer thereof Use in preparing drugs for inhibiting tyrosine kinase activity.
  • the present application provides a method for preventing or treating a disease or disorder related to tyrosine kinase, the method comprising administering to an individual in need a therapeutically effective amount of a compound of formula I of the present disclosure or a pharmaceutically acceptable compound thereof Salts, esters, solvates, prodrugs, active metabolites, crystals, stereoisomers, tautomers or geometric isomers or compounds comprising the formula I or pharmaceutically acceptable salts, esters, Pharmaceutical compositions of solvates, prodrugs, active metabolites, crystals, stereoisomers, tautomers or geometric isomers.
  • the present application provides a method for inhibiting the activity of tyrosine kinase, the method comprising administering the compound of formula I of the present disclosure or a pharmaceutically acceptable salt, ester, solvate, Prodrugs, active metabolites, crystals, stereoisomers, tautomers or geometric isomers or compounds comprising the formula I or pharmaceutically acceptable salts, esters, solvates, prodrugs, active Pharmaceutical compositions of metabolites, crystals, stereoisomers, tautomers or geometric isomers.
  • the method of inhibiting tyrosine kinase activity is performed in vivo or in vitro.
  • the method of inhibiting tyrosine kinase activity includes in vitro or in vivo tests.
  • the present application provides the compound of formula I of the present disclosure or a pharmaceutically acceptable salt, ester, solvate, prodrug, active metabolite, crystal, and stereoisomer of the present disclosure for inhibiting the activity of tyrosine kinase Conformer, tautomer or geometric isomer or comprising the compound of formula I or a pharmaceutically acceptable salt, ester, solvate, prodrug, active metabolite, crystal, stereoisomer, mutual Pharmaceutical compositions of mutamers or geometric isomers.
  • the present application provides a compound of formula I described in the present disclosure or a pharmaceutically acceptable salt, ester, solvate, prodrug, or active metabolite thereof for preventing or treating a disease or disorder related to tyrosine kinase , Crystals, stereoisomers, tautomers or geometric isomers, or compounds comprising the formula I or pharmaceutically acceptable salts, esters, solvates, prodrugs, active metabolites, crystals, stereo Pharmaceutical compositions of isomers, tautomers or geometric isomers.
  • the pharmaceutical composition of the present disclosure further includes a pharmaceutically acceptable carrier, excipient or adjuvant.
  • the medicament of the present disclosure further comprises another therapeutic agent for combination therapy.
  • the another therapeutic agent is selected from one or more of the following: cyclophosphamide, ifosfamide, vincristine, daunorubicin, doxorubicin, cytarabine, Mitoxantrone, dacarbazine, Idarubicin, Tretinoin, Prednisone, Dexamethasone, Mercaptopurine, Methotrexate, Paclitaxel, Melphalan, Long-acting Interferon, Venetog, Gram Zoltinib, Erlotinib, Ositinib, Ruxotinib, Afatinib, Erlotinib, Imatinib, Lapatinib, Bevacizumab, Trastuzumab, Li Tuximab, Cetuximab, Bonatumumab, Fludarabine, Gemcitabine, Decitabine,
  • the diseases associated with tyrosine kinases are diseases, disorders, and conditions that benefit from the inhibition or reduction of tyrosine kinase activity.
  • the tyrosine kinase includes Bcr-Abl tyrosine kinase and BTK tyrosine kinase.
  • the disease is selected from cancer.
  • the cancer is selected from the group consisting of: chronic myelogenous leukemia (CML), gastrointestinal stromal tumor (GIST), small cell lung cancer (SCLC), non-small cell lung cancer (NSCLC), multiple myeloma, solid Tumor, B-cell lymphoma, chronic lymphocytic leukemia (CLL), acute lymphocytic leukemia (ALL), non-Hodgkin’s lymphoma (NHL), small lymphocytic lymphoma (SLL), mantle cell lymphoma (MCL) ), melanoma, mastocytosis, germ cell tumors, acute myeloid leukemia (AML), marginal zone/diffuse large B-cell lymphoma, sarcoma, pancreatic cancer, malignant glioma, head and neck tumors, giant bulbs Proteinemia, follicular center lymphoma, prostate cancer, myelodysplastic syndrome, atherosclerotic myelodysplasia
  • CML
  • the cancer is selected from cancers that are resistant to the targets BCR-ABL and c-KIT by chemotherapeutic agents, and cancers that are resistant to imatinib.
  • the diseases, disorders, and conditions are selected from: bone metastases, hypercalcemia and/or osteoporosis; lung fibrosis; cardiovascular diseases or symptoms; mast cell-mediated inflammatory diseases; HTLV -1 related myelopathy/tropical spasmodic paralysis; complex regional pain syndrome (CRPS); weight loss or fat loss; arterial obstructive disease; ubiquitination; diseases or symptoms related to reduced sugar degradation; Fridreich co-help Disorders; Parkinson’s disease progression; Transplant rejection; Rheumatoid arthritis; Graft versus host disease; Autoimmune disease; Recurrent immune thrombocytopenic purpura; Pemphigus vulgaris; Systemic lupus erythematosus; Scleroderma Pulmonary interstitial fibrosis; and spontaneous urticaria.
  • the cardiovascular disease or symptom is a cardiovascular disease caused by RASopathy, or a congenital heart disease related to Noonan or Noonan syndrome.
  • the mast cell-mediated inflammatory disease is selected from osteoarthritis, asthma, chronic obstructive pulmonary disease, uveitis, aspirin exacerbated respiratory disease (AERD), and Parkinson's disease.
  • the compound of formula I of the present disclosure has higher kinase inhibitory activity than existing drugs such as dasatinib.
  • the inhibitor compounds of the present disclosure can inhibit the SRC kinase family ABL1, BTK and BTK (C481S) kinases. By inhibiting the effects of the above kinases, they can inhibit the proliferation of leukemia cells in CML and Ph+ALL bone marrow. However, normal red blood cells, white blood cells and platelets can continue to proliferate.
  • the inventions of the application improve the curative effect of the medicine, reduce the dosage, thereby reducing the toxic and side effects, and better provide clinical treatment for diseases such as immune diseases, tumors, neurological diseases, and rheumatoid arthritis.
  • the novel low-dose inhibitor of the present disclosure will provide broad application prospects for disease treatment.
  • the compounds of formula I were used for in vitro kinase inhibitory activity studies and human liver microsomal metabolism studies.
  • Positive control group use staurosporine (supplier is Med Chem), matrix solution is DMSO.
  • the initial concentration of the positive control group 20 ⁇ M
  • the response dose (M) is shown in the following table:
  • Buffer conditions 20mM Hepes (pH 7.5), 10mM MgCl 2 , 1mM EGTA, 0.02% Brij35, 0.02mg/ml BSA, 0.1mM Na 3 VO 4 , 2mM DTT, 1% DMSO
  • Substrate Amount of substrate in RXN supplier ABLtide 20 ⁇ M GenScript pEY 0.2mg/ml Sigma pEY 0.2mg/ml Sigma
  • the kinase activity data is expressed as the percentage of the remaining kinase activity in the test sample that reacts with the carrier (dimethyl sulfoxide).
  • the carrier dimethyl sulfoxide.
  • the compound of formula I inhibited ABL1, BTK, and BTK (C481S) activity significantly higher than dasatinib.
  • Buffer 0.05M phosphate buffer (pH 7.4)
  • Test compound verapamil, dasatinib, compound of formula I
  • liver microsomal protein, buffer, and test compound Transfer the liver microsomal protein, buffer, and test compound to a 1.5 ml incubation tube according to the volume in the experimental design table, incubate at 37°C for 5 min, and keep constant shaking.
  • Mobile phase mobile phase A is water containing 0.1% formic acid
  • mobile phase B is acetonitrile containing 0.1% formic acid.
  • the gradient is a gradient of 5-95% B every 5.1 min.
  • the amount of parent compound at each time is determined based on the peak area ratio (compound area/warfarin area).
  • V( ⁇ L/mg) Incubation volume ( ⁇ L)/Incubation protein amount (mg)

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Abstract

式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体或者包含所述式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体的药物组合物在制备预防或者治疗与酪氨酸激酶相关的疾病的药物中的用途。

Description

氟取代的2-氨基噻唑-5-芳香族甲酰胺的用途
相关申请的交叉引用
本申请要求于2019年8月20日向中国国家知识产权局提交的第201910767562.1号中国专利申请的优先权和权益，所述申请公开的内容通过引用整体并入本文中。
技术领域
本申请属于药物化学领域，具体涉及一种氟取代的2-氨基噻唑-5-芳香甲酰胺用于治疗和预防疾病和病症或其药物组合物在与酪氨酸激酶相关的疾病(例如免疫性疾病、肿瘤和神经疾病等疾病)中的预防或者治疗用途。
背景技术
蛋白激酶是一类催化蛋白质磷酸化，从而改变其底物活性或与其他蛋白结合能力的酶。激酶信号通路为最常见的可逆翻译后修饰形式，控制着细胞功能的许多方面。蛋白激酶异常活化是恶性肿瘤的主要标志，包括细胞增殖、存活、运动和代谢的改变，以及血管增生和逃避抗肿瘤免疫反应等疾病。
1.BCR-ABL
大量研究已经证实Bcr-Abl酪氨酸激酶在慢性粒细胞白血病(CML)发病机制中的作用，Bcr-Abl抑制剂为新的靶向治疗小分子药物的开发铺平了道路。伊马替尼(Imatinib)，博舒替尼(Bosutinib)，达沙替尼(Dasatinib)，尼洛替尼(Nilotinib)，拉多替尼(Radotinib)，和帕纳替尼(Ponatinib)已被批准用于治疗慢性粒细胞白血病嗜酸性粒细胞增多综合征、骨髓增生/骨髓增生性肿瘤、慢性粒细胞白血病、急性淋巴细胞白血病/淋巴细胞淋巴瘤。
尽管这些药物提高了肿瘤患者的生存率，同时这些药物也带来了严重不良反应，如严重的腹泻。这一问题尤其体现在胃和上消化道，因为口服后该部位的药物浓度较高。在该药物浓度下，选择性差的酪氨酸酶抑制剂(TKIs)能抑制胃肠道中大量表达的蛋白激酶，导致严重的常见剂量相关的毒性反应。
2.BTK
Bruton’s酪氨酸激酶(BTK)是一种非受体激酶，已经被证实为X-连锁无丙种球蛋白血症的遗传因素。BTK在致癌信号中起到了至关重要的作用，该信号对于许多B细胞恶性肿瘤的白血病细胞的增殖和生存有重要作用。因此，BTK是开发B细胞恶性肿瘤小分子抑制剂的关键靶点。
尽管目前已有批准用于肿瘤治疗的Bcr-Abl抑制剂，且能从根本上提高患者的平均生存率。但是新的基因突变和耐药形式也会出现在临床实践中，因此需要进一步开发新的抑制剂。大剂量药物可引起靶蛋白突变，导致活性丧失。仍需要寻找一种低剂量、能减少副作用和降低突变可能性的Bcr-Abl抑制剂和可逆的BTK抑制剂来满足医学需求。
发明内容
第一方面，本申请提供式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体或者包含所述式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体的药物组合物在制备预防或者治疗与酪氨酸激酶相关的疾病的药物中的用途，
Figure PCTCN2020110061-appb-000001
第二方面，本申请提供式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体或者包含所述式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体的药物组合物在制备抑制酪氨酸激酶活性的药物中的用途。
第三方面，本申请提供预防或者治疗与酪氨酸激酶相关的疾病的方法，所述方法包括向有需要的个体施用治疗有效量的式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体或者包含所述式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体的药物组合物。
第四方面，本申请提供抑制酪氨酸激酶活性的方法，所述的方法包括向个体或者其组织或细胞施用式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体或者包含所述式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体的药物组合物。在一些实施方案中，所述抑制酪氨酸激酶活性的方法在体内或体外进行。
第五方面，本申请提供式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体或者包含所述式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体的药物组合物，其用于抑制酪氨酸激酶活性的用途。
第六方面，本申请提供式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体或者包含所述式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体的药物组合物，其用于预防或者治疗与酪氨酸激酶相关的疾病的用途。
在上述任一方面的一些实施方案中，所述药物组合物还包括药学上可接受的载体、赋形剂或辅料。
在上述任一方面的一些实施方案中，所述药物进一步包含另一治疗剂用于联合治疗。在一些实施方案中，所述另一治疗剂选自以下中的一种或多种：环磷酰胺、异环磷酰胺、长春新碱、柔红霉素、阿霉素、阿糖胞苷、米托蒽醌、达卡巴嗪、伊达比星、维甲酸、强的松、地塞米松、巯嘌呤、甲氨蝶呤、紫杉醇、美法仑、长效干扰素、维奈托克、克唑替尼、埃罗替尼、奥西替尼、鲁索替尼、阿法替尼、厄洛替尼、伊马替尼、拉帕替尼、贝伐单抗、曲妥珠单抗、利妥昔单抗、西妥昔单抗、博纳吐单抗、氟达拉滨、吉西他滨、地西他滨、卡培他滨、苯达莫司汀、依维莫司、替西罗莫司、依托泊苷、阿霉素、粒细胞集落刺激因子、替莫唑胺、唑来膦酸、奥利沙铂、顺铂、卡铂和氟维司群等。
在上述任一方面的一些实施方案中，所述与酪氨酸激酶相关的疾病为受益于酪氨酸激酶活性的抑制或减少的疾病、病症和病况。
在上述任一方面的一些实施方案中，所述酪氨酸激酶包括Bcr-Abl酪氨酸激酶和BTK酪氨酸激酶。
在上述任一方面的一些实施方案中，所述疾病选自癌症。
在上述任一方面的一些实施方案中，所述癌症选自：慢性粒细胞白血病(CML)，胃肠间质瘤(GIST)，小细胞肺癌(SCLC)，非小细胞肺癌(NSCLC)，多发性骨髓瘤，实体瘤，B-细胞淋巴瘤，慢性淋巴细胞白血病(CLL)，急性淋巴细胞白血病(ALL)，非霍奇金淋巴瘤(NHL)，小淋巴细胞性淋巴瘤(SLL)，套细胞淋巴瘤(MCL)，黑色素瘤，肥大细胞增多症，生殖细胞肿瘤，急性髓细胞白血病(AML)，边缘区/弥漫性大B细胞淋巴瘤，肉瘤，胰腺癌，恶性胶质瘤，头颈部肿瘤，巨球蛋白血症，滤泡中心淋巴瘤，前列腺癌，骨髓增生异常综合征，动脉粥样硬化性骨髓增生，骨髓纤维化，嗜酸性粒细胞增多症，真性红细胞增多症，肝癌，晚期肉瘤，多形性胶质母细胞瘤，胶质肉瘤，恶性间皮瘤，黑色素瘤，鳞状细胞癌皮肤癌，神经内分泌肿瘤，胃肿瘤，B细胞急性淋巴细胞白血病，毛细胞白血病，淋巴浆细胞淋巴瘤，卵泡中心淋巴瘤，肾细胞癌，移行细胞癌，类癌肿瘤，T细胞淋巴瘤，转移性非小细胞肺癌，系统性肥大细胞增多症，转移性肾细胞癌，乳腺肿瘤，中枢神经系统肿瘤，结直肠肿瘤，转移性膀胱癌，转移性胰腺癌，转移性头颈癌，卵巢肿瘤和其组合。
在上述任一方面的一些实施方案中，所述癌症选自化学治疗剂对靶点BCR-ABL和c-KIT具有抗药性的癌症、以及对伊马替尼具有抗药性的癌症。
在上述任一方面的一些实施方案中，所述疾病、病症和病况选自：骨转移，高钙血症和/或骨质疏松；肺纤维疾病；心血管疾病或症状；肥大细胞介导的炎性疾病；HTLV-1相关性脊髓病/热带痉挛性瘫痪；复杂区域疼痛综合征(CRPS)；体重减轻或脂肪减少；动脉阻塞性疾病；泛素化；与降解糖功能降低相关的疾病或症状；Fridreich共济失调；帕金森病进展移植排斥反应；类风湿性关节炎；移植物抗宿主病；自身免疫性疾病；复发的免疫性血小板减少性紫癜；寻常型天疱疮；系统性红斑狼疮；硬皮病肺间质纤维化；和自发性荨麻疹。在一些实施方案中，所述心血管疾病或症状为RASopathy引起的心血管类疾病，或与Noonan或Noonan综合征相关的先天性心脏病。
在上述任一方面的一些实施方案中，所述肥大细胞介导的炎性疾病选自骨关节炎、哮喘、慢性阻塞性肺疾病、葡萄膜炎、阿司匹林加重呼吸道疾病(AERD)和帕金森氏病。
详细描述
定义
提供以下定义和方法用以更好地界定本申请以及在本申请实践中指导本领域普通技术人员。除非另作说明，术语按照相关领域普通技术人员的常规用法理解。本文所引用的所有专利文献、学术论文及其他公开出版物，其中的全部内容整体并入本文作为参考。
术语“任选”或“任选地”是指随后描述的事件或情况可能发生或可能不发生，该描述包括发生所述事件或情况和不发生所述事件或情况。
术语“药学上可接受的盐”是指保留了指定化合物的游离酸和游离碱的生物效力，并且在生物学或其它方面上没有不良作用的盐。本文所述的化合物可具有酸性或碱性基团，因此可与任意的多种无机碱或有机碱以及无机酸和有机酸反应，从而形成药学上可接受的盐。这些盐可以通过以下方法制备：在本申请化合物的最终分离和纯化过程中原位制备，或者通过本申请化合物的游离碱形式与适合的有机酸或无机酸单独反应，并分离由此形成的盐。药学上可接受的盐的实例包括通过本文所述化合物与无机酸或有机酸或无机碱或有机碱之间的反应而制备的盐。这些盐包括乙酸盐、丙烯酸盐、己二酸盐、海藻酸盐、天冬氨酸盐、苯甲酸盐、苯磺酸盐、硫酸氢盐、重亚硫酸盐、溴化物、丁酸盐、丁炔-1，4-二酸盐、樟脑酸盐、樟脑磺酸盐、辛酸盐、氯代苯甲酸盐、氯化物、柠檬酸盐、环戊烷丙酸盐、癸酸盐、葡萄糖酸盐、磷酸二氢盐、二硝基苯甲酸盐、十二烷基硫酸盐、乙磺酸盐、甲酸盐、延胡索酸盐、葡庚糖酸盐、甘油磷酸盐、乙醇酸盐、半硫酸盐、庚酸盐、己炔-1，6-二酸盐(hexyne-1，6-dioate)、羟基苯甲酸盐、y-羟基丁酸盐、盐酸盐、氢溴酸盐、氢碘酸盐、2-羟乙磺酸盐、碘化物、异丁酸盐、乳酸盐、马来酸盐、丙二酸盐、甲磺酸盐、扁桃酸盐、偏磷酸盐、甲氧基苯甲酸盐、甲基苯甲酸盐、磷酸一氢盐、1-萘磺酸盐、2-萘磺酸盐、烟酸盐、硝酸盐、双羟萘酸盐、果冻酸盐(pectinate)、过硫酸盐、3-苯丙酸盐、磷酸盐、苦味酸盐、特戊酸盐、丙酸盐、焦硫酸盐、焦磷酸盐、丙炔酸、邻苯二甲酸盐、苯乙酸盐、苯丁酸盐、丙磺酸盐、水杨酸盐、琥珀酸盐、硫酸盐、亚硫酸盐、辛二酸盐、癸二酸盐、磺酸盐、酒石酸盐、硫氰酸盐、对甲苯磺酸盐、十一酸盐(undeconate)和二甲苯磺酸盐。其它酸(如草酸)，尽管其本身在药学上是不可接受的，但可作为中间物在盐的制备过程中使用，以获得本申请的化合物及其在药学上可接受的酸加成盐(参见Berge et al.,J.Pharm.Sci.1977,66,1-19中的实施例)。此外，本文所述的可包括游离酸基团的化合物可与合适的碱反应(例如药学上可接受的金属阳离子的氢氧化物、碳酸盐或碳酸氢盐)，与氨反应，或与药学上可接受的有机伯胺、仲胺或叔胺反应。代表性的碱金属盐或碱土金属盐包括锂盐、钠盐、钾盐、钙盐、镁盐和铝盐等。碱的说明性实例包括氢氧化钠、氢氧化钾、氢氧化羟乙基三甲胺、碳酸钠和IV'(C 1-4烷基) 4等。用于形成碱加成盐的代表性有机胺包括乙胺、二乙胺、乙二胺、乙醇胺、二乙醇胺和哌嗪 等。应理解，本文所述化合物还包括其可能包含的任意碱性含氮基团的季铵化物。可通过季铵化反应获得水溶性或油溶性或可分散的产物。参见，例如上文Berge等人的文献。
术语“溶剂化物”是指一个组合了本申请化合物与溶剂分子所形成的一种溶剂物。在一些实施方式中，这种溶剂化物是一水合物，例如溶剂是水，本申请化合物与水形成的一水合物。
本发明化合物的酯是指那些在合理的医学判断范围内、适合用于与患者的组织接触而没有过多的毒性、刺激、过敏反应等的药学上可接受的酯。应当理解，含有羧基或羟基的式I化合物的酯包括体内可水解的酯，如在人体或动物体内被水解生成母体酸或醇的可药用酯。在本发明化合物的羧基基团(若存在)上形成的羧基的合适可药用酯包括如烷基酯(如C 1-6或C 1-4烷基酯)、环烷基酯(如C 3-12、C 3-8或C 3-6环烷基酯)、芳基烷基酯(如C 6-12或C 6-8芳基烷基酯)和杂芳基烷基酯(如C 6-12或C 6-8杂芳基烷基酯)等。
包含羟基基团的本发明化合物的体内可水解的酯包括无机酯，如磷酸酯、[α]-酰氧基烷基醚和与酯在体内水解分解得到母体羟基的结果相关的化合物。进一步合适的体内可水解的酯包括那些由氨基酸形成的酯。例如由化合物的羟基与氨基酸的羧酸反应形成的酯。进一步体内可水解酯包括磷酰胺酯，也包括其中任何游离羟基独立形成磷酰基或亚磷酸基酯的本发明化合物。
此外，本发明包括本发明化合物所有可能的晶形或多晶型物，包括其单独的多晶型物或任何比例的多种多晶型物的混合物。术语“多晶型物”是指本申请化合物存在于不同的晶格形式。
本文所用的术语“前药”包括为本领域的实践者所公知的酸衍生物或醇衍生物等。其中，酸衍生物例如通过母体酸与合适的醇的反应制备的酯、或通过母体酸与取代或未取代的胺的反应制备的酰胺、或酸酐、或混合酸酐等；醇衍生物例如选自醇酯、醇烷氧基化物、醇醚、羧酸、羧酸酯、磷酸化物以及它们的混合物等。由悬垂在本发明的化合物上酸性基团产生的简单的脂族或芳族酯、酰胺和酸酐以及羟基上的磷酸化物是具体的前药。
术语“活性代谢物”是指当化合物被代谢时形成的化合物的生物活性衍生物。
本申请的某些化合物可以具有不对称碳原子(立体中心)或双键。因此，外消旋体、非对映异构体、对映异构体、几何异构体和单个异构体都包括在本申请的范围之内。
当本申请的化合物含有烯属双键或其它几何不对称中心时，除非另有规定，它们包括E和Z几何异构体。同样地，所有的互变异构形式均包括在本申请的范围之内。
本申请的化合物可以存在特定的几何异构体或立体异构体形式。本申请设想所有的这类化合物，包括互变异构体、顺式和反式异构体、(-)-和(+)-对映体、(R)-和(S)-对映体、非对映异构体、(D)-异构体、(L)-异构体，及其外消旋混合物和其他混合物，例如对映异构体或非对映体富集的混合物，所有这些都属于本申请的范围之内。烷基等取代基中可以存在另外的不对称碳原子。所有这些异构体以及它们的混合物均包括在本申请的范围之内。
可以通过手性合成或手性试剂或者其他常规技术制备光学活性的(R)-和(S)-异构体以及D和L异构体。如果想得到本申请某化合物的一种对映体，可以通过不对称合成或者具有手性助剂的衍生作用来制备，其中将所得非对映体混合物分离，并且辅助基团裂开以提供纯的所需对映异构体。或者，当分子中含有碱性官能团(如氨基)或酸性官能团(如羧基)时，与适当的光学活性的酸或碱形成非对映异构体的盐，然后通过本领域所公知的常规方法进行非对映异构体拆分，然后回收得到纯的对映体。此外，对映异构体和非对映异构体的分离通常是通过使用色谱法完成的，所述色谱法采用手性固定相，并任选地与化学衍生法相结合(例如由胺生成氨基甲酸盐)。
本申请还包括与本文中记载的那些相同的，但一个或多个原子被原子量或质量数不同于自然中通常发现的原子量或质量数的原子置换的同位素标记的本申请化合物。可结合到本申请化合物的同位素的实例包括氢、碳、氮、氧、磷、硫、氟、碘和氯的同位素，诸如分别为 2H、 3H、 11C、 13C、 14C、 13N、 15N、 15O、 17O、 18O、 31P、 32P、 35S、 18F、 123I、 125I和 36Cl等。
某些同位素标记的本申请化合物(例如用 3H及 14C标记的那些)可用于化合物和/或底物组织分布分析中。氚化(即 3H)和碳-14(即 14C)同位素对于由于它们易于制备和可检测性是尤其优选的。正电子发射同位素，诸如 15O、 13N、 11C和 18F可用于正电子发射断层扫描(PET)研究以测定底物占有率。通常可以通过与公开于下文的方案和/或实施例中的那些类似的下列程序，通过同位素标记试剂取代未经同位素标记的试剂来制备同位素标记的本申请化合物。
此外，用较重同位素(诸如氘(即 2H))取代可以提供某些由更高的代谢稳定性产生的治疗优点(例如增加的体内半衰期或降低的剂量需求)，并且因此在某些情形下可能是优选的，其中氘取代可以是部分或完全的，部分氘取代是指至少一个氢被至少一个氘取代，所有这样的形式的化合物包含于本申请的范围内。
术语“受试者”、“患者”或“个体”是指患有疾病、病症或病况等的个体，包括哺乳动物和非哺乳动物。哺乳动物的实例包括但不限于哺乳动物纲的任何成员：人，非人的灵长类动物(例如黑猩猩和其它猿类和猴)；家畜，例如牛、马、绵羊、山羊、猪；家养动物，例如兔、狗和猫；实验室动物，包括啮齿类动物，例如大鼠、小鼠和豚鼠等。非人哺乳动物的实例包括但不限于鸟类和鱼类等。在本文提供的一个有关方法和组合物的实施方案中，所述哺乳动物为人。
术语“治疗”意为将本申请所述化合物或制剂进行给药以改善或消除疾病或与所述疾病相关的一个或多个症状，且包括：
(i)抑制疾病或疾病状态，即遏制其发展；
(ii)缓解疾病或疾病状态，即使该疾病或疾病状态消退。
术语“预防”意为将本申请所述化合物或制剂进行给药以预防疾病或与所述疾病相关的一个或多个症状，且包括：预防疾病或疾病状态在个体中出现，特别是当个体易患有该疾病状态，但尚未被诊断为已患有该疾病状态时。
术语“有效量”、“治疗有效量”或“药学有效量”是指服用后足以在某种程度上缓解所治疗的疾病或病症的一个或多个症状的至少一种药剂或化合物的量。其结果可以为迹象、症状或病因的消减和/或缓解，或生物系统的任何其它所需变化。例如，用于治疗的“有效量”是在临床上提供显著的病症缓解效果所需的包含本文公开化合物的组合物的量。可使用诸如剂量递增试验的技术测定适合于任意个体病例中的有效量。
术语“可接受的”是指对接受治疗的受试者的一般健康情况没有长期的有害影响。
术语“药学上可接受的”是指不影响本申请化合物的生物活性或性质的物质(如载体或稀释剂)，并且相对无毒，即该物质可施用于个体而不造成不良的生物反应或以不良方式与组合物中包含的任意组分相互作用。
术语“组合物”或“药物组合物”是指任选地混合有至少一种药学上可接受的化学成分的生物活性化合物，所述药学上可接受的化学成分包括但不限于载体、稳定剂、稀释剂、分散剂、悬浮剂、增稠剂和/或赋形剂。
术语“载体”是指相对无毒的化学化合物或试剂，其有助于将化合物引入到细胞或组织中。
术语“药学上可接受的辅料”是指对有机体无明显刺激作用，而且不会损害该活性化合物的生物活性及性能的那些辅料。合适的辅料是本领域技术人员熟知的，例如碳水化合物、蜡、水溶性和/或水可膨胀的聚合物、亲水性或疏水性材料、明胶、油、溶剂、水等。
词语“包括/包含(comprise)”及其英文变体例如comprises或comprising应理解为开放的、非排他性的意义，即“包括但不限于”。
本申请的药物组合物可通过将本申请的化合物与适宜的药学上可接受的辅料组合而制备，例如可配制成固态、半固态、液态或气态制剂，如片剂、丸剂、胶囊剂、粉剂、颗粒剂、锭剂、膏剂、糖浆剂、乳剂、悬浮剂、溶液剂、栓剂、注射剂、吸入剂、凝胶剂、微球及气溶胶等。
本申请化合物、其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体或者其药物组合物的典型的给药途径或施用途径包括但不限于口服、直肠、透黏膜、局部、经皮、吸入、肠胃外、舌下、阴道内、鼻内、眼内、腹膜内、肌内、皮下和静脉内给药。优选的给药途径是口服给药和注射给药。
本发明的化合物或组合物可按配方制造且作为以下剂型使用：供口服给药的片剂、胶囊或酏剂；供直肠给药的栓剂；供注射给药的无菌溶液，悬浮液；供透皮给药的贴片以及皮下沉积物等。注射剂可以下列常规形式制备：溶液或悬浮液、在注射前适合制成溶液或悬浮液的固体剂型、或乳剂。适合的赋形剂是，例如，水、盐水、葡萄糖、甘露醇、乳糖、卵磷脂、白蛋白、谷氨酸钠、半胱氨酸盐酸盐等。另外，如果需要，所述的注射用药物组合物可包含较少量的无毒性辅助物，例如湿润剂、pH缓冲剂等。如果需要，也可使用吸收增强剂(例如，脂质体)。
用于非肠道给药的制剂包括以水溶性形式存在的所述活性化合物的水溶液。另外， 所述活性化合物的悬浮液可制备成合适的油状注射悬液。适合的亲脂性溶剂或载体包括诸如芝麻油的脂肪油，或其它诸如大豆油、柚油或杏仁油的有机油，或诸如油酸乙酯或甘油三酯的合成的脂肪酸酯，或脂质体。水性注射悬液可包含增加所述悬液粘度的物质，例如羧甲基纤维素钠、山梨糖醇或葡聚糖。任意地，所述的混悬液可包含适合的稳定剂或提高所述化合物的溶解性的试剂，使得可制备高浓度的溶液。
本申请的药物组合物可以采用本领域已知的方法制备，如常规的混合法、溶解法、制粒法、制糖衣药丸法、磨细法、乳化法和冷冻干燥法等。
在一些实施方案中，本申请的药物组合物是口服形式。对于口服给药，可以通过将活性化合物与本领域熟知的药学上可接受的辅料或赋形剂混合来配制该药物组合物。这些辅料或赋形剂能使本申请的化合物被配制成片剂、丸剂、锭剂、糖衣剂、胶囊剂、粉剂、颗粒剂、液体、糖浆剂、乳剂、凝胶剂、浆剂、悬浮剂等，用于对患者的口服给药。
可以通过常规的混合、填充或压片方法来制备适于口服给药的固体药物组合物。例如，可通过下述方法获得固体形式的口服组合物：将活性化合物与固体辅料或赋形剂混合，任选地碾磨所得的混合物，如果需要则加入其它合适的辅料或赋形剂，然后将该混合物加工成颗粒，得到了片剂或糖衣剂的核心。合适的辅料或赋形剂包括但不限于：填充剂、粘合剂、稀释剂、崩解剂、润滑剂、助流剂、甜味剂或矫味剂等。例如用于口服的药物制剂可通过下述方法获得：将所述的活性化合物与固体赋形剂结合，任意地碾磨所得的混合物，以及加工该颗粒混合物，如果需要，在加入适合的辅助剂后得到了片剂或糖衣剂核。适合的赋形剂是，具体而言，诸如糖的填充剂，包括乳糖、蔗糖、甘露醇或山梨糖醇；纤维素制剂例如玉米淀粉、小麦淀粉、米淀粉、马铃薯淀粉、明胶、黄芪胶、甲基纤维素、羟丙基甲基纤维素、羧甲基纤维素钠、和\或者聚乙烯吡咯酮(PVP)。如果需要，可加入崩解剂，例如交联聚乙烯吡咯烷酮、琼脂或藻酸或诸如藻酸钠的藻酸盐。对糖衣剂核进行适合的包被。为了这个目的，可使用浓缩的糖溶液，该溶液可任意地包含阿拉伯胶、滑石、聚乙烯吡咯烷酮、聚羧乙烯凝胶、聚乙二醇和\或二氧化钛、紫胶漆溶液和适合的有机溶剂或溶剂混合物。为了识别或表示活性化合物剂量的不同组合的特征，可向片剂或糖衣剂包衣中加入染料或色素。使用本领域中公知的方法可制造这些制剂。
本文所述的化合物或组合物的所有施用方法中，每天给药的剂量可以例如为0.001-300mg/kg体重，如0.01-300mg/kg体重、或10-200mg/kg体重，以单独剂量或分开剂量的形式给药。
本申请的药物组合物还可适用于肠胃外给药，如合适的单位剂型的无菌溶液剂、混悬剂、乳剂或冻干产品。能够使用适当的赋形剂，例如填充剂、缓冲剂或表面活性剂。
本申请以Bcr-Abl和BTK为靶点，研发了一种激酶抑制剂，用于预防或治疗免疫性疾病、肿瘤和神经疾病等。
一方面，本申请提供式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体或者包含所述式I的化合物或其 药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体的药物组合物在制备预防或者治疗与酪氨酸激酶相关的疾病的药物中的用途，
Figure PCTCN2020110061-appb-000002
另一方面，本申请提供式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体或者包含所述式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体的药物组合物在制备抑制酪氨酸激酶活性的药物中的用途。
另一方面，本申请提供预防或者治疗与酪氨酸激酶相关疾病或病症的方法，所述方法包括向有需要的个体施用治疗有效量的本公开的式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体或者包含所述式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体的药物组合物。
又一方面，本申请提供抑制酪氨酸激酶活性的方法，所述的方法包括向个体或者其组织或细胞施用本公开的式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体或者包含所述式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体的药物组合物。在一些实施方案中，所述抑制酪氨酸激酶活性的方法在体内或体外进行。在一些实施方案中，所述抑制酪氨酸激酶活性的方法包括体外或者体内试验。
另一方面，本申请提供用于抑制酪氨酸激酶活性的本公开的所述式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体或者包含所述式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体的药物组合物。
另一方面，本申请提供用于预防或者治疗与酪氨酸激酶相关疾病或病症的本公开所述式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体或者包含所述式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体的药物组合物。
在一些实施方案中，本公开的所述药物组合物还包括药学上可接受的载体、赋形剂或辅料。
在一些实施方案中，本公开的所述药物进一步包含另一治疗剂用于联合治疗。在一些实施方案中，所述另一治疗剂选自以下中的一种或多种：环磷酰胺、异环磷酰胺、长春新碱、柔红霉素、阿霉素、阿糖胞苷、米托蒽醌、达卡巴嗪、伊达比星、维甲酸、强的松、地塞米松、巯嘌呤、甲氨蝶呤、紫杉醇、美法仑、长效干扰素、维奈托克、克唑替尼、埃罗替尼、奥西替尼、鲁索替尼、阿法替尼、厄洛替尼、伊马替尼、拉帕替尼、贝伐单抗、曲妥珠单抗、利妥昔单抗、西妥昔单抗、博纳吐单抗、氟达拉滨、吉西他滨、地西他滨、卡培他滨、苯达莫司汀、依维莫司、替西罗莫司、依托泊苷、粒细胞集落刺激因子、替莫唑胺、唑来膦酸、奥利沙铂、顺铂、卡铂和氟维司群等。
在一些实施方案中，所述与酪氨酸激酶相关的疾病为受益于酪氨酸激酶活性的抑制或减少的疾病、病症和病况。
在一些实施方案中，所述酪氨酸激酶包括Bcr-Abl酪氨酸激酶和BTK酪氨酸激酶。
在一些实施方案中，所述疾病选自癌症。
在一些实施方案中，所述癌症选自：慢性粒细胞白血病(CML)，胃肠间质瘤(GIST)，小细胞肺癌(SCLC)，非小细胞肺癌(NSCLC)，多发性骨髓瘤，实体瘤，B-细胞淋巴瘤，慢性淋巴细胞白血病(CLL)，急性淋巴细胞白血病(ALL)，非霍奇金淋巴瘤(NHL)，小淋巴细胞性淋巴瘤(SLL)，套细胞淋巴瘤(MCL)，黑色素瘤，肥大细胞增多症，生殖细胞肿瘤，急性髓细胞白血病(AML)，边缘区/弥漫性大B细胞淋巴瘤，肉瘤，胰腺癌，恶性胶质瘤，头颈部肿瘤，巨球蛋白血症，滤泡中心淋巴瘤，前列腺癌，骨髓增生异常综合征，动脉粥样硬化性骨髓增生，骨髓纤维化，嗜酸性粒细胞增多症，真性红细胞增多症，肝癌，晚期肉瘤，多形性胶质母细胞瘤，胶质肉瘤，恶性间皮瘤，黑色素瘤，鳞状细胞癌皮肤癌，神经内分泌肿瘤，胃肿瘤，B细胞急性淋巴细胞白血病，毛细胞白血病，淋巴浆细胞淋巴瘤，卵泡中心淋巴瘤，肾细胞癌，移行细胞癌，类癌肿瘤，T细胞淋巴瘤，转移性非小细胞肺癌，系统性肥大细胞增多症，转移性肾细胞癌，乳腺肿瘤，中枢神经系统肿瘤，结直肠肿瘤，转移性膀胱癌，转移性胰腺癌，转移性头颈癌，卵巢肿瘤和其组合。
在一些实施方案中，所述癌症选自化学治疗剂对靶点BCR-ABL和c-KIT具有抗药性的癌症、以及对伊马替尼具有抗药性的癌症。
在一些实施方案中，所述疾病、病症和病况选自：骨转移，高钙血症和/或骨质疏松；肺纤维疾病；心血管疾病或症状；肥大细胞介导的炎性疾病；HTLV-1相关性脊髓病/热带痉挛性瘫痪；复杂区域疼痛综合征(CRPS)；体重减轻或脂肪减少；动脉阻塞性疾病；泛素化；与降解糖功能降低相关的疾病或症状；Fridreich共济失调；帕金森病进展移植排斥反应；类风湿性关节炎；移植物抗宿主病；自身免疫性疾病；复发的免疫性血小板减少性紫癜；寻常型天疱疮；系统性红斑狼疮；硬皮病肺间质纤维化；和自发性荨麻疹。在一些实施方案中，所述心血管疾病或症状为RASopathy引起的心血管类疾病，或与Noonan或Noonan综合征相关的先天性心脏病。
在一些实施方案中，所述肥大细胞介导的炎性疾病选自骨关节炎、哮喘、慢性阻塞性肺疾病、葡萄膜炎、阿司匹林加重呼吸道疾病(AERD)和帕金森氏病。
本申请的各项发明提供下述一种或多种优势：
1.本公开的式I化合物具有高于现有药物如达沙替尼的激酶抑制活性。
2.体外肝微粒体试验显示：与现有药物如达沙替尼相比较，本公开的式I化合物半衰期更长，清除率更低。目前达沙替尼剂量是每天100毫克，而本公开的式I化合物比现有药物如达沙替尼的所用剂量低，给药间隔更长。
在纳摩尔浓度(nM)，本公开的抑制剂化合物能抑制SRC激酶家族ABL1，BTK和BTK(C481S)激酶，通过抑制上述激酶的作用，可抑制CML和Ph+ALL骨髓中白血病细胞的增殖，但正常红细胞、白细胞和血小板仍可继续增殖。
本申请的各项发明提高了药物疗效，降低了剂量从而降低了毒副作用，更好地为免疫疾病、肿瘤、神经疾病和类风湿关节炎等疾病提供临床治疗。本公开的新型低剂量抑制剂将为疾病治疗提供广阔的应用前景。
具体实施方式
下面结合实施例，对本发明的具体实施方式作进一步详细描述。以下实施例仅用于说明的目的，而非用来限制本申请的范围。
制备例1
式I化合物的合成:
Figure PCTCN2020110061-appb-000003
式I化合物的合成路线如下：
[5-(2-氯-4-氟-6-甲基苯基氨甲酰基)噻唑-2-基]-氨甲酸叔丁酯的合成
Figure PCTCN2020110061-appb-000004
N 2气保护下，将2-叔丁氧羰基氨基噻唑-5-羧酸24.4g(0.1mol)和0.5ml DMF(N，N-二甲基甲酰胺)加入250ml二氯甲烷中，缓慢滴加13ml草酰氯溶液(0.15mol)，反应2h，旋蒸除去溶剂，得到白色固体，将该固体溶于100ml无水二氯甲烷中，冰浴条件下，缓慢滴加至2-氯-4-氟-6-甲基苯胺17.5g(0.11mol)和N，N-二异丙基乙基胺38.8g(0.3mol)的二氯甲烷溶液中，N 2气保护下室温反应10h，减压蒸馏除去溶剂，加入25ml乙酸乙酯和25ml正己烷的混合溶剂搅拌2小时，抽滤，用乙酸乙酯淋洗，得到类白色粉末状固体35.2g，收率85％，纯度95％(ESI-MS(m/z)：[M+H] +，386。 1H NMR(DMSO-d 6)，δ：1.49(s，9H，-CH 3)，2.18(s，3H，-CH 3)，6.72(s，1H，芳香氢)，6.78(s，1H，芳香氢)， 8.14(s，1H，噻唑氢)，9.51(s，1H，-NH)，11.81(s，1H，-NH)。
2-氨基-N-(2-氯-4-氟-6-甲基苯基)-5-噻唑甲酰胺的合成
Figure PCTCN2020110061-appb-000005
将[5-(2-氯-4-氟-6-甲基苯基氨甲酰基)噻唑-2-基]-氨甲酸叔丁酯33.6g(0.087mol)加入到200ml二氯甲烷中，加入100ml三氟乙酸，室温搅拌6h，TLC检测反应完全，减压浓缩，将得到的油状物加入冰水中，用NaOH溶液调pH到10，充分搅拌至无油状物，再将pH回调至7，析出大量固体，抽滤，淋洗，干燥，得到淡黄色固体23.0g，收率92.5％。ESI-MS(m/z)：[M+H] +，286。 1H NMR(DMSO-d 6)，δ：2.21(s，3H，-CH 3)，6.71(s，1H，芳香氢)，6.76(s，1H，芳香氢)，7.63(s，2H，-NH 2)，7.88(s，1H，噻唑氢)，9.66(s，1H，-NH)。
N-(2-氯-4-氟-6-甲基苯基)-2-[(2-甲基-6-氯-4-嘧啶基)氨基]-5-噻唑甲酰胺的合成
Figure PCTCN2020110061-appb-000006
将2-氨基-N-(2-氯-4-氟-6-甲基苯基)-5-噻唑甲酰胺21.3g(0.075mol)溶于100mlDMF中，加入2-甲基-4，6-二氯嘧啶17.93g(0.11mol)和48.9g碳酸铯(0.15mol)，40℃保温反应12h，抽滤，滤液加入300ml冰水中，用稀盐酸调pH至6，搅拌析晶，抽滤，滤饼用乙酸乙酯淋洗，干燥，得到浅黄色固体29.5g，收率95.3％。ESI-MS(m/z)：[M+H] +，412。 1H NMR(DMSO-d 6)，δ：2.25(s，3H，-CH 3)，2.59(s，3H，-CH 3)，6.98(s，1H，嘧啶氢)，6.73(s，1H，芳香氢)，6.77(s，1H，芳香氢)，8.32(s，1H，噻唑氢)，10.01(s，1H，-NH)，12.21(s，1H，-NH)。
N-(2-氯-4-氟-6-甲基苯基)-2-[[6-[4-(2-羟乙基)-1-哌嗪基]-2-甲基-4-嘧啶基]氨基]-5-噻唑甲酰胺的合成
Figure PCTCN2020110061-appb-000007
称取N-(2-氯-4-氟-6-甲基苯基)-2-[(2-甲基-6-氯-4-嘧啶基)氨基]-5-噻唑甲酰胺28.2g(0.068mol)、N-羟乙基哌嗪44.3g(0.34mol)、N，N-二异丙基乙胺26.4g(0.204mol)，溶于250ml异丙醇中，升温至83℃回流反应8h，减压浓缩掉部分溶剂，冷却至室温，过滤，滤饼用乙醇和水1:1的混合溶液重结晶，得到白色粉末状固体31.36g，收率91.1％，纯度98％。ESI-MS(m/z)：[M+H] +，506。 1H NMR(DMSO-d 6)，δ：2.21(s，3H，-CH 3)， 2.41(s，3H，-CH 3)，2.43(t，2H，-CH 2)，2.48(t，4H，-CH 2)，3.52-3.54(m，4H，-CH 2)，3.55-3.56(m，2H，-CH 2)，4.48(s，1H，-OH)，6.06(s，1H，嘧啶氢)，6.73(s，1H，芳香氢)，6.77(s，1H，芳香氢)，8.22(s，1H，噻唑氢)，9.87(s，1H，-NH)，11.45(s，1H，-NH)。
实施例1
采用式I化合物进行体外激酶抑制活性研究、人肝微粒体代谢研究。
1.激酶抑制活性研究
1.1实验方法
1.1.1试验药物配制
实验组：分别用50μl DMSO溶解式I化合物与达沙替尼，最终药物浓度为10mM。
阳性对照组：采用星形孢菌素(供应商为Med Chem)，基质溶液为DMSO。
1.1.2给药剂量
实验组药物起始浓度：1μM
阳性对照组起始浓度：20μM
化合物孵育时间：15min
ATP浓度：10μM
反应时间：2h
反应剂量(M)如下表所示：
Figure PCTCN2020110061-appb-000008
1.1.3实验条件与过程
缓冲液条件：20mM Hepes(pH 7.5)，10mM MgCl 2，1mM EGTA，0.02％Brij35，0.02mg/ml BSA，0.1mM Na 3VO 4，2mM DTT，1％DMSO
备注：需要的辅酶因子单独加入到每个激酶反应中。
测定条件：
Figure PCTCN2020110061-appb-000009
底物 RXN中的底物量 供应商
ABLtide 20μM GenScript
pEY 0.2mg/ml Sigma
pEY 0.2mg/ml Sigma
反应过程：
a)在新制备的反应缓冲液中制备指示底物。
b)在上述底物溶液中添加任何必需的辅酶因子。
c)在底物溶液中加入指示激酶，轻轻混合。
d)采用Echo 550将试验药物加至激酶反应混合物中。
e)将 33P-ATP(放射性比度0.01μCi/μl)加入到反应混合物中并启动反应。
f)激酶反应在室温下孵育120分钟。
g)该反应在P81离子交换纸上显示斑点。
h)使用0.75％磷酸清洗过滤器。
i)测量滤纸上残留的放射性磷酸化底物。
1.2数据分析
激酶活性数据表示为测试样品中剩余激酶活性与载体(二甲基亚砜)反应的百分比。使用Prism4软件(GraphPad)获得IC 50值和曲线拟合。
1.3实验结果
Figure PCTCN2020110061-appb-000010
1.4实验结论
式I化合物抑制ABL1、BTK、BTK(C481S)活性明显比达沙替尼高。
2.肝微粒体代谢实验
2.1实验方法
2.1.1孵育条件
底物浓度：1.0μM
缓冲液：0.05M磷酸盐缓冲液(pH 7.4)
人肝微粒体：
Figure PCTCN2020110061-appb-000011
UltraPool TM HLM 150，20mg/mL
微粒蛋白浓度：0.25mg/mL
NADPH浓度：1.0mM
总反应体积：850μL
孵育温度：37℃
预孵育时间：5min(未添加NADPH)
取样时间：0，5，10，15，20，30min(对照组为0，15，30min)
取样体积：每个时间点取样85μL
淬灭试剂：85μL乙腈含0.25μM华法林
测试化合物：维拉帕米，达沙替尼，式I化合物
2.1.2实验设计
Figure PCTCN2020110061-appb-000012
2.1.3实验过程
a)采用乙腈：水(50：50)将10mM测试化合物的DMSO溶液稀释成100μM。
b)将微粒体蛋白用50mM磷酸盐缓冲盐稀释成0.5mg/mL并置于冰浴中(人肝微粒体浓度为20mg/ml)。
c)配制2.5mM NADPH溶液。
d)按实验设计表中的体积依次移取肝微粒体蛋白、缓冲液、测试化合物至1.5ml孵育管中，在37℃孵育5min，并保持恒定的振摇。
e)在250μL淬灭小瓶中加入85μL淬灭试剂，并置于冰浴上。
f)通过加入NADPH后开始启动反应，立即取样85μL，并在淬灭小瓶中控制孵育与淬灭，混合均匀，此为0min取样点。样品加盖，在4℃下涡旋并离心。
g)淬灭溶液置于4℃下10min用于沉淀蛋白。
h)其他取样点的操作类似0min取样操作。
i)将样品淬灭反应混溶液彻底混合均匀，并在室温下14000rpm离心10min。
j)移取(～100μl)上层清液用于LC-MS分析。
2.2 LC-MS分析方法
仪器：
Figure PCTCN2020110061-appb-000013
ACQUITY HPLC-Xevo G2-XS QTof
色谱柱：ACQUITY
Figure PCTCN2020110061-appb-000014
BEH C 18柱2.1×50mm，1.7μm
流动相：流动相A为含0.1％甲酸的水，流动相B为含0.1％甲酸的乙腈。梯度为每5.1min按5～95％B的梯度。
流速：100μl/min。
根据峰面积比(化合物面积/华法林面积)确定每个时间的母体化合物的量。
2.3计算公式
固有清除率(CL int)通过以下公式计算。
清除速率常数(k)＝-斜率(1/min)
半衰期(t 1/2)＝0.693/k
V(μL/mg)＝孵育体积(μL)/孵育蛋白量(mg)
固有清除率(CL int)(μL/min/mg)＝V×0.693/t 1/2
2.4实验结果
Figure PCTCN2020110061-appb-000015
2.5实验结论
体外肝微粒体试验显示本公开的式I化合物与达沙替尼相比较半衰期较长，清除率较低，可延长给药间隔，减少用药次数。
上文中已经用一般性说明及具体实施方案对本发明作了详尽的描述，但在本发明基础上，可以对之作一些修改或改进，这对本领域技术人员而言是显而易见的。因此，在不偏离本发明精神的基础上所做的这些修改或改进，均属于本发明要求保护的范围。

Claims (12)

  1. 式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体或者包含所述式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体的药物组合物在制备预防或者治疗与酪氨酸激酶相关的疾病的药物中的用途，
    Figure PCTCN2020110061-appb-100001
  2. 式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体或者包含所述式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体的药物组合物在制备抑制酪氨酸激酶活性的药物中的用途，
    Figure PCTCN2020110061-appb-100002
  3. 预防或者治疗与酪氨酸激酶相关的疾病的方法，所述方法包括向有需要的个体施用治疗有效量的式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体或者包含所述式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体的药物组合物，
    Figure PCTCN2020110061-appb-100003
  4. 抑制酪氨酸激酶活性的方法，所述的方法包括向个体或者其组织或细胞施用式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体或者包含所述式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、 活性代谢物、晶体、立体异构体、互变异构体或几何异构体的药物组合物；任选地，所述方法在体内或体外进行，
    Figure PCTCN2020110061-appb-100004
  5. 式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体或者包含所述式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体的药物组合物，其用于抑制酪氨酸激酶活性的用途，
    Figure PCTCN2020110061-appb-100005
  6. 式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体或者包含所述式I的化合物或其药学上可接受的盐、酯、溶剂化物、前药、活性代谢物、晶体、立体异构体、互变异构体或几何异构体的药物组合物，其用于预防或者治疗与酪氨酸激酶相关的疾病的用途，
    Figure PCTCN2020110061-appb-100006
  7. 如权利要求1或2所述的用途，或者如权利要求3或4所述的方法，或者如权利要求5或6所述的用于所述用途的化合物或组合物，其中所述药物进一步包含另一治疗剂用于联合治疗；
    优选地，所述另一治疗剂选自以下中的一种或多种：环磷酰胺、异环磷酰胺、长春新碱、柔红霉素、阿霉素、阿糖胞苷、米托蒽醌、达卡巴嗪、伊达比星、维甲酸、强的松、地塞米松、巯嘌呤、甲氨蝶呤、紫杉醇、美法仑、长效干扰素、维奈托克、克唑替尼、埃罗替尼、奥西替尼、鲁索替尼、阿法替尼、厄洛替尼、伊马替尼、拉帕替尼、贝伐单抗、曲妥珠单抗、利妥昔单抗、西妥昔单抗、博纳吐单抗、氟达拉滨、吉西他滨、地西他滨、卡培他滨、苯达 莫司汀、依维莫司、替西罗莫司、依托泊苷、粒细胞集落刺激因子、替莫唑胺、唑来膦酸、奥利沙铂、顺铂、卡铂和氟维司群。
  8. 如权利要求1或7所述的用途，或者如权利要求3或7所述的方法，或者如权利要求6或7所述的用于所述用途的化合物或组合物，其中：
    所述与酪氨酸激酶相关的疾病为受益于酪氨酸激酶活性的抑制或减少的疾病、病症和病况。
  9. 如权利要求1、2和7-8中任一项所述的用途，或者如权利要求3、4和7-8中任一项所述的方法，或者如权利要求5、6和7-8中任一项所述的用于所述用途的化合物或组合物，其中：
    所述酪氨酸激酶包括Bcr-Abl酪氨酸激酶和BTK酪氨酸激酶。
  10. 如权利要求1和7-9中任一项所述的用途，或者如权利要求3和7-9中任一项所述的方法，或者如权利要求6和7-9中任一项所述的用于所述用途的化合物或组合物，其中所述疾病选自癌症，
    优选地，所述癌症选自：慢性粒细胞白血病(CML)，胃肠间质瘤(GIST)，小细胞肺癌(SCLC)，非小细胞肺癌(NSCLC)，多发性骨髓瘤，实体瘤，B-细胞淋巴瘤，慢性淋巴细胞白血病(CLL)，急性淋巴细胞白血病(ALL)，非霍奇金淋巴瘤(NHL)，小淋巴细胞性淋巴瘤(SLL)，套细胞淋巴瘤(MCL)，黑色素瘤，肥大细胞增多症，生殖细胞肿瘤，急性髓细胞白血病(AML)，边缘区/弥漫性大B细胞淋巴瘤，肉瘤，胰腺癌，恶性胶质瘤，头颈部肿瘤，巨球蛋白血症，滤泡中心淋巴瘤，前列腺癌，骨髓增生异常综合征，动脉粥样硬化性骨髓增生，骨髓纤维化，嗜酸性粒细胞增多症，真性红细胞增多症，肝癌，晚期肉瘤，多形性胶质母细胞瘤，胶质肉瘤，恶性间皮瘤，黑色素瘤，鳞状细胞癌皮肤癌，神经内分泌肿瘤，胃肿瘤，B细胞急性淋巴细胞白血病，毛细胞白血病，淋巴浆细胞淋巴瘤，卵泡中心淋巴瘤，肾细胞癌，移行细胞癌，类癌肿瘤，T细胞淋巴瘤，转移性非小细胞肺癌，系统性肥大细胞增多症，转移性肾细胞癌，乳腺肿瘤，中枢神经系统肿瘤，结直肠肿瘤，转移性膀胱癌，转移性胰腺癌，转移性头颈癌，卵巢肿瘤和其组合。
  11. 如权利要求10所述的用途，或者如权利要求10所述的方法，或者如权利要求10所述的用于所述用途的化合物或组合物，其中所述癌症选自化学治疗剂对靶点BCR-ABL和c-KIT具有抗药性的癌症、以及对伊马替尼具有抗药性的癌症。
  12. 如权利要求8所述的用途，或者如权利要求8所述的方法，或者如权利要求8所述的用于所述用途的化合物或组合物，其中所述疾病、病症和病况选自：骨转移，高钙血症和/或骨质疏松；肺纤维疾病；心血管疾病或症状；肥大细胞介导的炎性疾病；HTLV-1相关性脊髓病/热带痉挛性瘫痪；复杂区域疼痛综合征(CRPS)；体重减轻或脂肪减少；动脉阻塞性疾病；泛 素化；与降解糖功能降低相关的疾病或症状；Fridreich共济失调；帕金森病进展移植排斥反应；类风湿性关节炎；移植物抗宿主病；自身免疫性疾病；复发的免疫性血小板减少性紫癜；寻常型天疱疮；系统性红斑狼疮；硬皮病肺间质纤维化；和自发性荨麻疹；
    优选地，所述心血管疾病或症状为RASopathy引起的心血管类疾病，或与Noonan或Noonan综合征相关的先天性心脏病；
    优选地，所述肥大细胞介导的炎性疾病选自骨关节炎、哮喘、慢性阻塞性肺疾病、葡萄膜炎、阿司匹林加重呼吸道疾病(AERD)和帕金森氏病。
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